Open access peer-reviewed Edited Volume

Molecular Cloning

Selected Applications in Medicine and Biology

Edited by Gregory G. Brown

McGill University, Canada

The development of molecular cloning technology in the early 1970s created a revolution in the biological and biomedical sciences that extends to this day. The contributions in this book provide the reader with a perspective on how pervasive the applications of molecular cloning have become. The contributions are organized in sections based on application, and range from cancer biology and immunology to plant and evolutionary biology. The chapters also cover a wide range of technical approaches, such as positional cloning and cutting edge tools for recombinant protein expression. This book should appeal to many researchers, who should find its information useful for advancing their fields.

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Molecular CloningSelected Applications in Medicine and BiologyEdited by Gregory G. Brown

Published: October 12th 2011

DOI: 10.5772/898

ISBN: 978-953-307-398-9

eBook (PDF) ISBN: 978-953-51-5161-6

Copyright year: 2011

Books open for chapter submissions

64949 Total Chapter Downloads

6 Crossref Citations

16 Web of Science Citations

21 Dimensions Citations


Open access peer-reviewed

1. Screening of Bacterial Recombinants: Strategies and Preventing False Positives

By Sriram Padmanabhan, Sampali Banerjee and Naganath Mandi


Open access peer-reviewed

2. Non-Viral Vehicles: Principles, Applications, and Challenges in Gene Delivery

By Abbas Padeganeh, Mohammad Khalaj-Kondori, Babak Bakhshinejad and Majid Sadeghizadeh


Open access peer-reviewed

3. Subcloning and Expression of Functional Human Cathepsin B and K in E. coli: Characterization and Inhibition by Flavonoids

By Lisa Wen, Soe Tha, Valerie Sutton, Keegan Steel, Franklin Rahman, Matthew McConnell, Jennifer Chmielowski, Kenneth Liang, Roxana Obregon, Jessica LaFollette, Laura Berryman, Ryan Keefer, Michael Bordowitz, Alice Ye, Jessica Hunter, Jenq-Kuen Huang and Rose M. McConnell


Open access peer-reviewed

4. Molecular Cloning and Overexpression of WAP Domain of Anosmin-1 (a-WAP) in Escherichia coli

By Srinivas Jayanthi, Beatrice Kachel, Jacqueline Morris, Igor Prudovsky and Thallapuranam K. Suresh Kumar


Open access peer-reviewed

5. Effects of Two Novel Peptides from Skin of Lithobates Catesbeianus on Tumor Cell Morphology and Proliferation

By Rui-Li ZHAO, Jun-You HAN, Wen-Yu HAN, Hong-Xuan HE and Ji-Fei MA


Open access peer-reviewed

6. Molecular Cloning of Immunoglobulin Heavy Chain Gene Translocations by Long Distance Inverse PCR

By Takashi Sonoki


Open access peer-reviewed

7. Identification of Molecules Involved in the Vulture Immune Sensing of Pathogens by Molecular Cloning

By Elena Crespo, José de la Fuente and José M. Pérez de la Lastra


Open access peer-reviewed

8. Molecular Cloning, Characterization, Expression Analysis and Chromosomal Localization of the Gene Coding for the Porcine αIIb Subunit of the αIIbβ3 Integrin Platelet Receptor

By Gloria Esteso, Ángeles Jiménez-Marín, Gema Sanz, Juan José Garrido and Manuel Barbancho


Open access peer-reviewed

9. Molecular Cloning, Expression, Purification and Immunological Characterization of Proteins Encoded by Regions of Difference Genes of Mycobacterium tuberculosis

By Shumaila Nida Muhammad Hanif, Rajaa Al-Attiyah and Abu Salim Mustafa


Open access peer-reviewed

10. Molecular Toxinology – Cloning Toxin Genes for Addressing Functional Analysis and Disclosure Drug Leads

By Gandhi Rádis-Baptista


Open access peer-reviewed

11. Molecular Cloning, Expression, Function, Structure and Immunoreactivities of a Sphingomyelinase D from Loxosceles adelaida, a Brazilian Brown Spider from Karstic Areas

By Denise V. Tambourgi, Giselle Pidde-Queiroz, Rute M. Gonçalves-de-Andrade, Cinthya K. Okamoto, Tiago J. Sobreir, Paulo S. L. de Oliveira, Mário T. Murakami and Carmen W. van den Berg


Open access peer-reviewed

12. Cloning the Ribokinase of Kinetoplastidae: Leishmania Major

By Patrick Ogbunude, Joy Ikekpeazu, Joseph Ugonabo, Michael Barrett and Patrick Udeogaranya


Open access peer-reviewed

13. Genome Based Vaccines Against Parasites

By Yasser Shahein and Amira Abouelella


Open access peer-reviewed

14. Phosphagen Kinase System of the Trematode Paragonimus westermani: Cloning and Expression of a Novel Chemotherapeutic Target

By Blanca R. Jarilla and Takeshi Agatsuma


Open access peer-reviewed

15. Molecular Cloning, Expression Pattern, and Phylogenetic Analysis of the Lysyl-tRNA Synthetase Gene from the Chinese Oak Silkworm Antheraea pernyi

By Yan-Qun Liu and Li Qin


Open access peer-reviewed

16. Molecular Cloning and Characterization of Fe-Superoxide Dismutase (Fe-SOD) from the Fern Ceratopteris thalictroides

By Chen Chen and Quanxi Wang


Open access peer-reviewed

17. Cloning and Characterization of a Candidate Gene from the Medicinal Plant Catharanthus roseus Through Transient Expression in Mesophyll Protoplasts

By Patrícia Duarte, Diana Ribeiro, Gisela Henriques, Frédérique Hilliou, Ana Sofia Rocha, Francisco Lima, Isabel Amorim and Mariana Sottomayor


Open access peer-reviewed

18. Positional Cloning in Brassica napus: Strategies for Circumventing Genome Complexity in a Polyploid Plant

By Gregory G. Brown and Lydiane Gaborieau


Edited Volume and chapters are indexed in

  • Worldcat
  • OpenAIRE
  • Google Scholar
  • AZ ebsco
  • Base
  • CNKI

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