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Open access peer-reviewed Edited Volume

Cell Culture

Edited by Radwa Ali Mehanna

Alexandria University

Cell culture is one of the major tools used in cellular and molecular biology, delivering an excellent model for studying the normal physiology and biochemistry of cells. This book covers some advanced aspects in cell culture methodologies. The book has four sections discussing different types of cell culture models, including 3D cell culture techniques, their advantages, and limitations in comparison to traditional 2D culturing; cell viability, autophagy, in vitro toxicity tests and live cell imaging; stem cell culture for cell-based therapeutics; and specific applications and methodologies for hybrid cell lines and cancer models. This book provides a comprehensive overview of some of the advanced cell culture methodologies and applications. It serves as a valuable source for scientists, researchers, clinicians and students.

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Cell CultureEdited by Radwa Ali Mehanna

Published: January 23rd 2019

DOI: 10.5772/intechopen.73709

ISBN: 978-1-78984-867-0

Print ISBN: 978-1-78984-866-3

eBook (PDF) ISBN: 978-1-83881-748-0

Copyright year: 2019

Books open for chapter submissions

14788 Total Chapter Downloads

10 Crossref Citations

20 Web of Science Citations

42 Dimensions Citations


Open access peer-reviewed

1. 2D vs. 3D Cell Culture Models for In Vitro Topical (Dermatological) Medication Testing

By Arezou Teimouri, Pollen Yeung and Remigius Agu


Open access peer-reviewed

2. Two-Dimensional (2D) and Three-Dimensional (3D) Cell Culturing in Drug Discovery

By Jitcy Saji Joseph, Sibusiso Tebogo Malindisa and Monde Ntwasa


Open access peer-reviewed

3. Time-Lapse Microscopy

By John L. Collins, Bart van Knippenberg, Kai Ding and Alexander V. Kofman


Open access peer-reviewed

4. Cell-Based Assays for Evaluation of Autophagy in Cancers

By Siew-Wai Pang, Noel Jacques Awi, Hooi-Yeen Yap and Sin-Yeang Teow


Open access peer-reviewed

5. In Vitro Toxicity Testing of Nanomaterials

By Ansie Martin and Angshuman Sarkar


Open access peer-reviewed

6. Culturing Adult Stem Cells for Cell-Based Therapeutics: Neuroimmune Applications

By Victoria Moreno-Manzano and Elisa Oltra García


Open access peer-reviewed

7. Morphological Comparison of Stem Cells Using Two- Dimensional Culture and Spheroid Culture

By Sae Kyung Min, Hyunjin Lee, Minji Kim and Jun-Beom Park


Open access peer-reviewed

8. Authenticating Hybrid Cell Lines

By Raymond W. Nims, Amanda Capes-Davis, Christopher Korch and Yvonne A. Reid


Open access peer-reviewed

9. Air Pouch Model: An Alternative Method for Cancer Drug Discovery

By Moisés Armides Franco-Molina, Silvia Elena Santana-Krímskaya and Cristina Rodríguez-Padilla


Open access peer-reviewed

10. Optimization of the Self-Assembly Method for the Production of Psoriatic Skin Substitutes

By Alexe Grenier, Isabelle Gendreau and Roxane Pouliot


Open access peer-reviewed

11. Monolayers of Carbohydrate-Containing Lipids at the Water- Air Interface

By Bishal Nepal and Keith J. Stine


Open access peer-reviewed

12. Erythropoiesis and Megakaryopoiesis in a Dish

By Eszter Varga, Marten Hansen, Emile van den Akker and Marieke von Lindern


Edited Volume and chapters are indexed in

  • Worldcat
  • OpenAIRE
  • Google Scholar
  • AZ ebsco
  • Base
  • CNKI

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