About the book
Nowadays, methods of fluorescence analysis play a leading role in the investigation of biological objects. Actually, they are the only non-destructive methods for investigation of living cells and microorganisms in vivo. Using intrinsic (or self) fluorescence as well as artificial fluorescence these methods allows us deep insight into mechanisms underlying the physiological and biochemical processes. In the last years a large variety of novel research techniques were elaborated basing on the intrinsic and artificial fluorescence. The book Fluorescent Methods for Investigation of Living Cells and Microorganisms covers a wide range of modern experimental techniques such as: fluorescent microscopy, fluorescent spectroscopy, confocal laser scanning microscopy (FRAP, FRET, microscopic spectroscopy, etc.), flow cytofluorometry, delayed fluorescence excitation spectroscopy, pulse-amplitude-modulation fluorometry, fluorescent dyes staining methods, gene analysis. This book aims to give an overview of practical and theoretical aspects of fluorescent methods and their successfull application for investigation of static and dynamic processes in living cells and microorganisms in vivo and in situ.