Open access peer-reviewed Edited Volume

Fluorescent Methods for Investigation of Living Cells and Microorganisms

Natalia Grigoryeva

Saint-Petersburg Scientific Research Centre for Ecological Safety of Russian Academy of Sciences


Fluorescent Microscopy Fluorescent Spectroscopy Delayed Fluorescence PAM Fluorimetry Flow Cytofluorometry Natural Fluorescence Artificial Fluorescence Remote Sensing Photosynthetic Apparatus Luminescence Staining Methods Fluorescent Dyes

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About the book

Nowadays, methods of fluorescence analysis play a leading role in the investigation of biological objects. Actually, they are the only non-destructive methods for investigation of living cells and microorganisms in vivo. Using intrinsic (or self) fluorescence as well as artificial fluorescence these methods allows us deep insight into mechanisms underlying the physiological and biochemical processes. In the last years a large variety of novel research techniques were elaborated basing on the intrinsic and artificial fluorescence. The book Fluorescent Methods for Investigation of Living Cells and Microorganisms covers a wide range of modern experimental techniques such as: fluorescent microscopy, fluorescent spectroscopy, confocal laser scanning microscopy (FRAP, FRET, microscopic spectroscopy, etc.), flow cytofluorometry, delayed fluorescence excitation spectroscopy, pulse-amplitude-modulation fluorometry, fluorescent dyes staining methods, gene analysis. This book aims to give an overview of practical and theoretical aspects of fluorescent methods and their successfull application for investigation of static and dynamic processes in living cells and microorganisms in vivo and in situ.

Publishing process

Book initiated and editor appointed

Date completed: November 11th 2019

Applications to edit the book are assessed and a suitable editor is selected, at which point the process begins.

Chapter proposals submitted and reviewed

Deadline Extended: Open for Submissions

Potential authors submit chapter proposals ready for review by the academic editor and our publishing review team.

Approved chapters written in full and submitted

Deadline for full chapters: January 31st 2020

Once approved by the academic editor and publishing review team, chapters are written and submitted according to pre-agreed parameters

Full chapters peer reviewed

Review results due: April 20th 2020

Full chapter manuscripts are screened for plagiarism and undergo a Main Editor Peer Review. Results are sent to authors within 30 days of submission, with suggestions for rounds of revisions.

Book compiled, published and promoted

Expected publication date: June 19th 2020

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About the editor

Natalia Grigoryeva

Saint-Petersburg Scientific Research Centre for Ecological Safety of Russian Academy of Sciences

Dr. Grigoryeva Natalia (Ph.D. in Radiophysics) is presently working as a senior research fellow in Saint-Petersburg Scientific Research Centre for Ecological Safety of Russian Academy of Sciences, Russia. Besides, she is an official scientific consultant of the Department of Nano- and Microelectronics and the Department of Software Engineering and Computer Applications in Saint-Petersburg Electrotechnical University. She is also a reviewer for several scientific journals. She published about 100 conference papers, peer-reviewed journal articles, book chapters and monographs. She has delivered talks as an invited speaker at national and international conferences. In the last five years, she is involved in the research of cyanobacteria and the development of fluorescence methods of their investigation. She worked out several in vivo fluorescent techniques for investigation of the influence of weak external actions on a physiological state of cyanobacteria. Besides, she recently elaborated an original method of classification of cyanobacteria using single-cell self-fluorescence spectra based on neural networks application. Her research interests include both environmental and biotechnological aspects of cyanobacterial applications.

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Book chapters authored 3

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