Open access peer-reviewed Edited Volume

Fluorescence Methods for Investigation of Living Cells and Microorganisms

Edited by Natalia Grigoryeva

St. Petersburg Federal Research Center of Russian Academy of Sciences, Scientific-Research Centre for Ecological Safety

Fluorescence methods play a leading role in the investigation of biological objects. They are the only non-destructive methods for investigating living cells and microorganisms in vivo. Using intrinsic and artificial fluorescence methods provides deep insight into mechanisms underlying physiological and biochemical processes. This book covers a wide range of modern methods involved in experimental biology. It illustrates the use of fluorescence microscopy and spectroscopy, confocal laser scanning microscopy, flow cytometry, delayed fluorescence, pulse-amplitude-modulation fluorometry, and fluorescent dye staining protocols. This book provides an overview of practical and theoretical aspects of fluorescence methods and their successful application in the investigation of static and dynamic processes in living cells and microorganisms.

Read more >
Knowledge Unlatched

This book has been funded by Knowledge Unlatched. More information on this initiative can be found on

Order hardcopy
Fluorescence Methods for Investigation of Living Cells and MicroorganismsEdited by Natalia Grigoryeva

Published: September 30th 2020

DOI: 10.5772/intechopen.83296

ISBN: 978-1-83968-040-3

Print ISBN: 978-1-83968-039-7

eBook (PDF) ISBN: 978-1-83968-041-0

Copyright year: 2020

Books open for chapter submissions

7505 Total Chapter Downloads

1 Crossref Citations

6 Dimensions Citations


Open access peer-reviewed

1. Studying Cyanobacteria by Means of Fluorescence Methods: A Review

By Natalia Yu. Grigoryeva


Open access peer-reviewed

2. Far-Red Light Absorbing Photosynthetic Pigments in Cyanobacteria: Steady-State Fluorescence Detection, Time-Resolved Fluorescence Spectroscopy, and Confocal Laser Scanning Microscopy

By Svetlana Averina, Ekaterina Senatskaya and Alexander Pinevich


Open access peer-reviewed

3. Influence of the Wavelength of Excitation and Fluorescence Emission Detection on the Estimation of Fluorescence-Based Physiological Parameters in Different Classes of Photosynthetic Organisms

By Stefano Santabarbara, William Remelli, Anastasia A. Petrova and Anna Paola Casazza


Open access peer-reviewed

4. The Use of Fluorescence Microscopy to Assess the Suppression of the Development of Cyanobacteria under the Influence of Allelochemicals of Aquatic Macrophytes

By Evgeny Kurashov, Larisa Kapustina, Julia Krylova and Galina Mitrukova


Open access peer-reviewed

5. Fluorescence Behavior of Phytoplankton Blooms by Time-Correlated Single-Photon Counting (TCSPC)

By Helena C. Vasconcelos, Joao A. Lopes, Maria João Pereira and Afonso Silva Pinto


Open access peer-reviewed

6. Investigation on the Chlorophyll-a Content of Phytoplankton in the Sea of Azov and the Don River by the Fluorescence Method

By Pavel Plyaka, Galina Glushchenko, Viktoriya Gerasyuk, Alexey Kleshchenkov, Klim Grigorenko, Margarita Shevchenko, Yuriy Yurasov, Georgy Valov, Igor Tron, Gayane Popovyan and Sergey Berdnikov


Open access peer-reviewed

7. Fluorescence Fluctuation Techniques for the Investigation of Structure-Function Relationships of G-Protein-Coupled Receptors

By Robert T. Youker and Danielle Voet


Open access peer-reviewed

8. Study of the Fluorescence Intensity of Histones HP1gamma, H3K9me3, H3K27me3, and Histone Deacetylases SIRT1 and SIRT6 in Dermal Fibroblasts of Patients with Breast Cancer and Patients at Risk of Cancer

By Mirya Kuranova, Aleksandra Nozdracheva, Nadezhda Pleskach, Daria Pasekova and Aleksey Manikhas


Open access peer-reviewed

9. Mammalian Cell Viability Methods in 3D Scaffolds for Tissue Engineering

By Benjamin Gantenbein, Andreas S. Croft and Marie Larraillet


Open access peer-reviewed

10. Detection of Apoptosis in Cancer Cells Using Heat Shock Protein 70 and p53 Antibody Conjugated Quantum Dot Nanoparticles

By Lev B. Matyushkin, Olga A. Aleksandrova, Anna O. Drobintseva, Igor M. Kvetnoy, Yuliya S. Krylova, Yaroslav Y. Marchenko, Dmitriy S. Mazing, Vyacheslav A. Moshnikov, Sergey F. Мusikhin, Boris P. Nikolaev, Victoriya O. Polyakova, Maxim A. Shevtsov and Ludmila Y. Yakovleva


Open access peer-reviewed

11. Fluorescence Methods for the Analysis of Microtubule/Microfilament Involvement in the Regulation of Endothelial Barrier Function

By Anton S. Shakhov and Irina B. Alieva


Open access peer-reviewed

12. Fluorescent Nanoagents for Biomedical Applications

By Dmitry Korolev, Maria Istomina, Anton Belorus, Artem Brovko, Dmitry Sonin, Galina Shulmeyster, Natalya Evreinova and Vyacheslav Moshnikov


Open access peer-reviewed

13. Getting Charged for the Mitochondrial Uptake

By Ivo Crnolatac


Open access peer-reviewed

14. Confocal Laser Scanning Microscopy of Living Cells

By Alexey Moshkov


Open access peer-reviewed

15. A Protocol for Minimal Single Protein Labeling with CyDye Fluors for Live Cell Internalization Assays

By Antonio J. Castro, Alfonso Clemente and Juan de Dios Alché


Open access peer-reviewed

16. Fluorogenic Polyfunctional Coumarin-Based Chemosensors for Multianalyte Detection

By Alexander Dubonosov and Vladimir Bren


Open access peer-reviewed

17. Fluorescent Nanomaterials for Cellular Imaging

By Kalyan Sundar Ghosh and Anchal Sharma


Open access peer-reviewed

18. Diffusion Theory for Cell Membrane Fluorescence Microscopy

By Minchul Kang


Open access peer-reviewed

19. Special Cuvettes for Spectrofluorimeters

By Nikolai Vekshin


Open access peer-reviewed

20. Pore-Forming Proteins: Fluorescent Dyes to Study the Channel Functionality and Biophysical Properties

By Guilherme Teixeira and Robson Faria


Edited Volume and chapters are indexed in

  • Worldcat
  • OpenAIRE
  • Google Scholar
  • AZ ebsco
  • Base
  • CNKI

Order a hardcopy of the Edited Volume

Free shipping with DHL Express

Hardcover (ex. VAT)£139

Order now

Residents of European Union countries need to add a Book Value-Added Tax Rate based on their country of residence. Institutions and companies, registered as VAT taxable entities in their own EU member state, will not pay VAT by providing IntechOpen with their VAT registration number. This is made possible by the EU reverse charge method.

Special discount for IntechOpen contributors

All IntechOpen contributors are offered special discounts starting at 40% OFF available through your personal dashboard

Login and purchase