Open access peer-reviewed Edited Volume

Cell-Free Protein Synthesis

Edited by Manish Biyani

Tokyo University of Science, Japan

The Nobel Prize in Medicine 1968 for interpretation of the genetic code and its function in protein synthesis and in Chemistry 2009 for studies of the structure and function of the ribosome highlighted the ground-breaking experiment performed on May 15, 1961 by Nirenberg and Matthaei and their principal breakthrough on the creation of "cell-free protein synthesis (CFPS) system". Since then the continuous technical advances have revitalized CFPS system as a simple and powerful technology platform for industrial and high-throughput protein production. CFPS yields exceed grams protein per liter reaction volume and offer several advantages including the ability to easily manipulate the reaction components and conditions to favor protein synthesis, decreased sensitivity to product toxicity, batch reactions last for multiple hours, costs have been reduced orders of magnitude, and suitability for miniaturization and high-throughput applications. With these advantages, there is continuous increasing interest in CFPS system among biotechnologists, molecular biologists and medical or pharmacologists.

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Cell-Free Protein SynthesisEdited by Manish Biyani

Published: October 10th 2012

DOI: 10.5772/2955

ISBN: 978-953-51-0803-0

eBook (PDF) ISBN: 978-953-51-5328-3

Copyright year: 2012

Books open for chapter submissions

13307 Total Chapter Downloads

9 Crossref Citations

15 Web of Science Citations

22 Dimensions Citations


Open access peer-reviewed

1. Ribosomes from Trypanosomatids: Unique Structural and Functional Properties

By Maximiliano Juri Ayub, Walter J. Lapadula, Johan Hoebeke and Cristian R. Smulski


Open access peer-reviewed

2. On the Emergence and Evolution of the Eukaryotic Translation Apparatus

By Greco Hernández


Open access peer-reviewed

3. Evolutionary Molecular Engineering to Efficiently Direct in vitro Protein Synthesis

By Manish Biyani, Madhu Biyani, Naoto Nemoto and Yuzuru Husimi


Open access peer-reviewed

4. Protein Synthesis in vitro: Cell-Free Systems Derived from Human Cells

By Kodai Machida, Mamiko Masutan and Hiroaki Imataka


Open access peer-reviewed

5. Solid-Phase Cell-Free Protein Synthesis to Improve Protein Foldability

By Manish Biyani and Takanori Ichiki


Open access peer-reviewed

6. Cumulative Specificity: A Universal Mechanism for the Initiation of Protein Synthesis

By Tokumasa Nakamoto and Ferenc J. Kezdy


Open access peer-reviewed

7. Protein Synthesis and the Stress Response

By Assaf Katz and Omar Orellana


Edited Volume and chapters are indexed in

  • Worldcat
  • OpenAIRE
  • Google Scholar
  • AZ ebsco
  • Base
  • CNKI

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