Open access peer-reviewed Edited Volume

Patch Clamp Technique

Edited by Kaneez Fatima Shad

University of Technology Sydney

This book is a stimulating and interesting addition to the collected works on Patch clamp technique. Patch Clamping is an electrophysiological technique, which measures the electric current generated by a living cell, due to the movement of ions through the protein channels present in the cell membrane. The technique was developed by two German scientists, Erwin Neher and Bert Sakmann, who received the Nobel Prize in 1991 in Physiology for this innovative work. Patch clamp technique is used for measuring drug effect against a series of diseases and to find out the mechanism of diseases in animals and plants. It is also most useful in finding out the structure function activities of compounds and drugs, and most leading pharmaceutical companies used this technique for their drugs before bringing them for clinical trial. This book deals with the understanding of endogenous mechanisms of cells and their receptors as well as advantages of using this technique. It covers the basic principles and preparation types and also deals with the latest developments in the traditional patch clamp technique. Some chapters in this book take the technique to a next level of modulation and novel approach. This book will be of good value for students of physiology, neuroscience, cell biology and biophysics.

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Patch Clamp TechniqueEdited by Kaneez Fatima Shad

Published: March 23rd 2012

DOI: 10.5772/2086

ISBN: 978-953-51-0406-3

eBook (PDF) ISBN: 978-953-51-5255-2

Copyright year: 2012

Books open for chapter submissions

43222 Total Chapter Downloads

16 Crossref Citations

27 Web of Science Citations

32 Dimensions Citations


Open access peer-reviewed

1. Patch ClampTechnique for Looking at Serotonin Receptors in B103 Cell Lines: A Black Box Test

By K. Fatima-Shad and K. Bradley


Open access peer-reviewed

2. Intracellular Signaling Pathways Integrating the Pore Associated with P2X7R Receptor with Other Large Pores

By L.G.B. Ferreira, R.A.M. Reis, L.A. Alves and R.X. Faria


Open access peer-reviewed

3. Patch Clamp Study of Neurotransmission at Single Mammalian CNS Synapses

By Norio Akaike


Open access peer-reviewed

4. Single-Channel Properties and Pharmacological Characteristics of KATP Channels in Primary Afferent Neurons

By Takashi Kawano


Open access peer-reviewed

5. Regulation of Renal Potassium Channels by Protein Kinases and Phosphatases

By Manabu Kubokawa


Open access peer-reviewed

6. BK Channels – Focus on Polyamines, Ethanol/Acetaldehyde and Hydrogen Sulfide (H2S)

By Anton Hermann, Guzel F. Sitdikova and Thomas M. Weiger


Open access peer-reviewed

7. From Action Potential-Clamp to "Onion-Peeling" Technique – Recording of Ionic Currents Under Physiological Conditions

By Ye Chen-Izu, Leighton T. Izu, Peter P. Nanasi and Tamas Banyasz


Open access peer-reviewed

8. Electrophysiological Techniques for Mitochondrial Channels

By Rainer Schindl and Julian Weghuber


Open access peer-reviewed

9. Patch-Clamp Analysis of Membrane Transport in Erythrocytes

By Guillaume Bouyer, Serge Thomas and Stéphane Egée


Open access peer-reviewed

10. Electrical Membrane Properties in the Model Leishmania-Macrophage

By Marcela Camacho


Open access peer-reviewed

11. Cardiac Channelopathies: Disease at the Tip of a Patch Electrode

By Brian P. Delisle


Open access peer-reviewed

12. Gating Charge Movement in Native Cells: Another Application of the Patch Clamp Technique

By Oscar Vivas, Isabel Arenas and David E. García


Open access peer-reviewed

13. Use of Patch Clamp Electrophysiology to Identify Off-Target Effects of Clinically Used Drugs

By Lioubov I. Brueggemann and Kenneth L. Byron


Open access peer-reviewed

14. Role of Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) in Modulating Vascular Smooth Muscle Cells by Activating Large-Conductance Potassium Ion Channels

By Donald K. Martin, Christopher G. Schyvens, Kenneth R. Wyse, Jane A. Bursill, Robert A. Owe-Young, Peter S. Macdonald and Terence J. Campbell


Open access peer-reviewed

15. Perforated Patch Clamp in Non-Neuronal Cells, the Model of Mammalian Sperm Cells

By Jorge Parodi and Ataúlfo Martínez-Torres


Open access peer-reviewed

16. Drug Screening and Drug Safety Evaluation by Patch Clamp Technique

By Yan Long and Zhiyuan Li


Open access peer-reviewed

17. Enhanced Patch-Clamp Technique to Study Antimicrobial Peptides and Viroporins, Inserted in a Cell Plasma Membrane with Fully Inactivated Endogenous Conductances

By Marco Aquila, Mascia Benedusi, Alberto Milani and Giorgio Rispoli


Edited Volume and chapters are indexed in

  • Worldcat
  • OpenAIRE
  • Google Scholar
  • AZ ebsco
  • Base
  • CNKI

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