Open access peer-reviewed Edited Volume

Methodological Advances in the Culture, Manipulation and Utilization of Embryonic Stem Cells for Basic and Practical Applications

Edited by Craig Atwood

University of Wisconsin–Madison, United States of America

Pluripotent stem cells have the potential to revolutionise medicine, providing treatment options for a wide range of diseases and conditions that currently lack therapies or cures. This book describes methodological advances in the culture and manipulation of embryonic stem cells that will serve to bring this promise to practice.

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Methodological Advances in the Culture, Manipulation and Utilization of Embryonic Stem Cel...Edited by Craig Atwood

Published: April 26th 2011

DOI: 10.5772/2149

ISBN: 978-953-307-197-8

eBook (PDF) ISBN: 978-953-51-6432-6

Copyright year: 2011

Books open for chapter submissions

59060 Total Chapter Downloads

13 Crossref Citations

26 Web of Science Citations

32 Dimensions Citations


Open access peer-reviewed

1. Embryonic Stem Cells: from Blastocyst to in vitro Differentiation

By Genesia Manganelli, Annalisa Fico and Stefania Filosa


Open access peer-reviewed

2. Mouse Embryonic Stem Cells Basics from a Fertilized Zygote to These Promising Pluripotent Stem Cells

By Carol Fagundez, Mónica Loresi, Stella Delcourt and Pablo Argibay


Open access peer-reviewed

3. Effective Derivation and Manipulation of Mouse Embryonic Stem Cells

By Peng Zhang, Xinglong Wu, Pengbo Wang and Xiangyun Li


Open access peer-reviewed

4. Artificial Acellular Feeder Layer: An Advanced Engineered Extracellular Matrix for Stem Cell Culture

By Amranul Haque, Masato Nagaoka, Xiao-Shan Yue, Stephen A. Duncan and Toshihiro Akaike


Open access peer-reviewed

5. Biomaterials for In Vitro Expansion of Embryonic Stem Cells

By Makoto Sakuragi and Yoshihiro Ito


Open access peer-reviewed

6. Surface Engineering to Control Embryonic Stem Cell Fate

By Shohreh Mashayekhan and Jun-ichi Miyazaki


Open access peer-reviewed

7. Preservation of Embryonic Stem Cells

By Xiaoming He


Open access peer-reviewed

8. Topographically and Chemically Modified Surfaces for Expansion or Differentiation of Embryonic Stem Cells

By Lotte D’Andrea Markert, Jette Lovmand, Mogens Duch and Finn Skou Pedersen


Open access peer-reviewed

9. Directed Differentation of Human Embryonic Stem Cells in Combination of Biomaterials

By Huihui Wang, Xiaojing Xu, Rong Ye and Huanxiang Zhang


Open access peer-reviewed

10. Non-invasive Imaging of Human Embryonic Stem Cells Derived Endothelial Cells Using Reporter Genes

By Yizhou Zheng, Deling Kong and Zongjin Li


Open access peer-reviewed

11. Fourier Transform Infrared Microspectroscopy as a Tool for Embryonic Stem Cell Studies

By Diletta Ami, Paolo Mereghetti, Antonino Natalello and Silvia M. Doglia


Open access peer-reviewed

12. Reprogrammed Parthenogenetic ES Cells - New Choice for Regenerative Medicine

By Takuro Horii and Izuho Hatada


Open access peer-reviewed

13. Reprogramming Somatic Cells by Fusion with Embryonic Stem Cells: Present Status and Prospects in Regenerative Medicine

By Jinnuo Han and Kuldip S. Sidhu


Open access peer-reviewed

14. Use of Transposon-Transposase Systems for Stable Genetic Modification of Embryonic Stem Cells

By Tamás I. Orbán, Ágota Apáti, Zsuzsanna Izsvák, Zoltán Ivics and Balázs Sarkadi


Open access peer-reviewed

15. Gene Transduction Approaches in Human Embryonic Stem Cells

By David Brafman and Karl Willert


Open access peer-reviewed

16. Pluripotent Stem Cells Induced from Testicular Tissue of a Man with Klinefelter Syndrome (47, XXY) by Four Transcription Factors (OCT4, SOX2, KLF4, and C-MYC)

By Hideyuki Kobayashi


Open access peer-reviewed

17. Application of Magnet-based Nanofection in Embryonic Stem Cell Research

By Ssang-Goo Cho, Sachin Honguntikar and Hyun Joo Lee


Open access peer-reviewed

18. Chromosome Engineering in Mouse Embryonic Stem Cells: Addition and Elimination of Targeted Chromosomes

By Masako Tada


Open access peer-reviewed

19. Microarray Analysis of Undifferentiated and Differentiated Human Pluripotent Stem Cells

By Jane Synnergren and Peter Sartipy


Open access peer-reviewed

20. Tracking the Structure of Protein Interaction Network via Multiple Genetic Perturbations on Mouse Embryonic Stem Cells — Implementation of the Entropy Maximization Principle

By Lei Mao, Rossella De Cegli, Mario Lauria, Grit Nebrich, Jean Maurice Delabar, Yann Herault, Gilda Cobellis and Joachim Klose


Open access peer-reviewed

21. Establishment of Embryonic Stem Cells and Generation of Genetically Modified Rats

By Masaki Kawamata and Takahiro Ochiya


Open access peer-reviewed

22. Rat Embryonic Stem Cells: Establishment and Their Use for Transgenesis

By Masumi Hirabayashi and Shinichi Hochi


Open access peer-reviewed

23. Assessment of Embryotoxicity and Teratogenicity by the Embryonic Stem Cell Test

By Kazuaki Nakamura, Shinji Kusakawa and Akito Tanoue


Open access peer-reviewed

24. Application of Embryonic Stem Cells as a Novel Tool in Drug Screening

By Gi Jin Kim


Open access peer-reviewed

25. Human Pluripotent Stem Cell-Derived Neuronal Networks:Their Electrical Functionality and Usability for Modelling and Toxicology

By Riikka Äänismaa, Laura Ylä-Outinen, Jarno E. Mikkonen and Susanna Narkilahti


Open access peer-reviewed

26. A Novel Strategy for Drug Discovery and Development by Analyzing the Behavior of ES Cells Cultured on TOSHI (Tissue/Organ Sections for Histopathology)–Substrata

By Toshiaki Takezawa


Edited Volume and chapters are indexed in

  • Worldcat
  • OpenAIRE
  • Google Scholar
  • AZ ebsco
  • Base
  • CNKI

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