Photosynthesis is a crucial process for plants on earth that changes light energy to chemical energy. Virus infection can cause dramatic photosynthesis changes: respiration and the translocation of carbohydrates and other substances around the host plant. Chlorosis in virus-infected leaves like Barley Yellow Dwarf Virus (BYDV- PAV).infection can result from damage to chloroplasts resulting from inhibition of photosynthetic activity. Our present study combines TEM and chlorophyll-level content in the presence of Gold nanoparticles (AuNPS) to explore the repair mechanism for the yellowing leaf symptom development caused by infection with BYDV- PAV by illustrating TEM micrographs; showing fragmentized grana, deformation of the myelin like bodies (MLB), many vesicles; osmiophilic lipid granules/plastoglobulus, starch body, and plasmolysis in the chloroplast, distribution of AuNPs & VLPs near and inside the chloroplast. Mitochondria, Double-membrane-bound organelle, Distorted mitochondrion, Amorphous inclusion bodies.
- Barley Yellow Dwarf Virus (BYDV-PAV)
- Gold nanoparticles (AuNPs)
- Starch granules (S)
- osmiophilic lipid granules/plastoglobulus (OG)
- Myelin like bodies (MLB)
- Portentous content
- Fragmentized grana. Mitochondria
- Double-membrane-bound organelle
- Distorted mitochondrion
- Amorphous inclusion bodies
BYDV-PAV causes cytological alterations, physiological and biochemical, including the restriction of photosynthate transportation, phloem degeneration, and creating a nutritionally , and the formation of specific cytological inclusions . In the susceptible wheat and after BYDV-GAV infection, drop expressions of chlorophyll biosynthesis and chloroplast was noticed due to related genes and altered expression of the ABA mentioned above. Chloroplast in the healthy cells has normal densities of thylakoid in chloroplast (Figure 1(A)–(D)). The lower chlorophyll content, fragmentized chloroplasts, ROS accumulation, and slower growth could be engaged to ET signaling and ROS related genes, consequently resulting in leaf yellowing and plant dwarfing (Figure 2).
Studies by several laboratories of TMV-infected tobacco plants suggest that the uptake of viral CP causes chlorosis in these plants by thylakoid. R. Beachy’s lab observed that the CP aggregates in the thylakoid membrane. Resulting the intense free radical damage to the organelle refer to pull apart photosystem II (the water-splitting reaction of photosynthesis). In vitro experiments, M. Zaitlin’s laboratory noticed that uptake of the CP did not accomplish the host’s normal pathways for protein import using isolated chloroplasts. Instead, a feature of the CP, when it was assembled into multimeric disks (an intermediate structure in TMV virion assembly), let it be taken up randomly into the chloroplasts and the thylakoid membrane eventually. The single amino acid in the CP determines its uptake efficiency into chloroplasts, and K. Lehto has detected the relative severity of chlorosis caused by different TMV strains. Changes in photosynthesis, respiration, and carbohydrate translocation generally cause localized aggregation and depletion of starch, the primary stock carbohydrate of most plants . Common cytopathological effects of BYDV-PAV infection are restricted to the phloem of host plants cleared , where they are seen via EM in the cytoplasm, nuclei, and vacuoles of infected sieve elements, companion and parenchyma cells. Vesicles containing filaments and inclusions containing virus particles the infection and subsequent death of phloem cells inhibits translocation as Figure 2(A)–(C), slows plant growth, and induces loss of chlorophyll typical symptoms. Plants convert only 2–4% of the available energy in radiation into new plant growth .
2. Chemical energy production of nanoparticles
Metal nanoparticles can induce the efficiency of chemical energy production in photosynthetic systems, Figure 5(C). Nano-anatase TiO2 has a photocatalyzed characteristic, improves the light absorbance, the transformation from light energy to electrical, chemical energy, and induces carbon dioxide assimilation. TiO2NPs protect chloroplast from aging for long time illumination [6, 7]. Nano-anatase TiO2 enhances the photosynthetic carbon assimilation with potentially activate Rubisco (a complex of Rubisco , which promotes Rubisco carboxylation, thereby increasing the growth of plants [8, 9] studied the impact of nano-anatase on the molecular mechanism of carbon reaction and postulated the marker gene’s induction for Rubisco activase (RCA) mRNA by nano-anatase. Enhancing protein levels and activities of Rubisco activated the improvement of the Rubisco carboxylation and the high rate of photosynthetic carbon reaction. The exogenous application of TiO2NPs improves the net photosynthetic rate, conductance to water, and transpiration rate in plants . Nano-anatase promoted heartily whole chain electron transport , photoreduction activity of photosystem II, O2-evolving, and photophosphorylation activity of chlorophyll under both visible and ultraviolet light. The AuNPs and Ag nanocrystals bind to the chlorophyll in the photosynthetic reaction center , forming a novel hybrid system, which may build ten times more excited electrons plasmon resonance fast electron–hole separation. The enhancement mechanisms may help the design of artificial light-harvesting systems. Nanomaterials can generate ROS, affect lipid peroxidation, as reported . The significant biochemical and molecular effect on membrane permeability and fluidity, which due to previous nanomaterials impact making cells more susceptible to osmotic stress and failure to nutrient uptake. A series of metabolic activities such as soil and water which perceived the stress through the growth matrix [14, 15, 16] triggered to alleviate the metal stressors . To deal with the situation, in the first step, to prohibit metal entry through the expense of energy, plants modulating their action actively. In the second step, modulating transporters in the plasma membrane prevent further entry of the metal into the cytosol, so that the intracellular build-up of metal ions does not exceed the threshold concentration, Figure 3(A,B, and D). The plant system has developed several well-synchronized systems to Elementary Flux Mode (EFM) to prevent metal ion build-up from the cellular milieu .
AuNPs significantly increased vegetative growth and seed production in both noncrops (
The changes in root system function such as root length or biomass caused by BYDV infection is unknown, although root tips are far from the photosynthate source but suffered from reduced translocation. Potential reductions in root system function of BYDV-infected plants could play a crucial role in grain yield loss because root systems supply shoot organs with fundamental mineral nutrients  due to BYDV infection, and a susceptible wheat cultivar showed a 72% reduction in photosynthetic capacity. On the other hand, a moderately tolerant wheat cultivar exhibited only a 60% reduction in photosynthesis . Photosynthesis was reduced by 25% per gram of fresh tissue weight in BYDV-infected plants . Many studies have shown that virus infection can trigger severe chlorophyll breakdown within the host. An imbalance between biosynthesis and catabolic turnover of green pigments in plant tissues indicates profound inhibition of the photosynthesis process [27, 28, 29, 30]. Phytohormone levels are also altered following BYDV infection.  undertook a detailed study of abscisic acid, jasmonic acid, methyl jasmonate, methyl salicylate (MS), and salicylic acid (SA) following BYDV infection. They followed the phytohormones over time and with different watering conditions than undamaged controls and seedlings infested with non-viruliferous aphids. Total hormone concentrations in BYDV-infected plants were more significant than those in sham-treated and control plants. SA was higher in infected plants, but MS was low.
Turnip yellow mosaic virus (TYMV) replication machinery interacts with the outer membrane of infected cell chloroplasts , like in our study in Figure 5(A,B,D, and E). In TYMV-infected cells, the chloroplasts adopt a cup-shaped form aggregate and swell. Two TYMV proteins are targeted to the chloroplast membrane and are involved in these processes, the 66 and 140 kDa proteins. The 140 kDa protein induces chloroplast aggregation and induces the invagination of the outer chloroplast membrane to form small peripheral vesicles, which are the sites of TYMV replication. Invaginations of endoplasmic and the plastid membrane were precise in our micrographs; Figures 2(A) and (B) and 4(A).
Mitochondria and plastids in infected cells became degenerate in a way similar to those in uninfected maturing sieve elements. Negative staining preparation for Electron Microscopy is used for staining virus particles and the morphological and cytological side of healthy (Figure 6) and treated leaves . Some alterations were detected in mitochondria of fully infected phloem cells, Figure 7(A) and (B). Furthermore, virus-specific vesicles with the limiting membrane were developed in the cytoplasm.
Barley Yellow Dwarf Virus
Barley Yellow Dwarf Virus
Reactive Oxygen Species
Tobacco mosaic virus
Transmission electron microscope
Messenger Ribonucleic Acid
Turnip yellow mosaic virus
Myelin like bodies
Irregular starch granules
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