Cellular ultrastructure micrographs revealed striking changes resulting from the Barley Yellow Dwarf Virus (BYDV-PAV) infection in Electron microscopy. In the cytoplasm, the Gold nanoparticles (AuNPs) may bind with different cytoplasmic organelles and interfere with the treated site’s metabolic processes. The micrographs of the treated plant leave with AuNPs showing; Endosomes, amorphous bodies, slender filaments fibers, myelin bodies with a high concentration of virus particles, and Gold Nanoparticles distributed in a circulated shape in the cytoplasm with virus particles.
- Cytoplasmic matrix
- Barley Yellow Dwarf Virus (BYDV-PAV)
- Gold nanoparticles (AuNPs)
- Amorphous bodies
- Slender filaments fibers
- Myelin bodies
In subsequent stages, significant accumulations of virus particles as inFigures 1(E) and 2(D) and filaments were visible outside the nucleus throughout the cytoplasm, as in Figure 1(D). When the virus was common throughout the cytoplasm, vesicles with fibrils were often still clustered around vacuole like areas in the cytoplasm, Figure 1(A)–(C).
In addition to that, amorphous cellular inclusion bodies, was shown in Figures 1(B) and 2(A)–(C), numerous filamentous shapes appeared in Figure 1(E). Endosomes as in Figure 2(A)–(D), deformed invagination of the cell wall, Figure 2(A) virus localization, and cytopathic alterations of barley
2. Viroplasms and gold nanoparticles
Inclusions of virus-derived material in virus-infected cells termed viroplasms. It may be quasicrystalline, amorphous, or crystalline, in appearance the accumulation of nascent virions in the host cell from some inclusions aggregates of viral proteins, Figures 1(D) and (E) and 2(A)–(C). Large aggregates of convoluted, branched, tubular bodies and myelin bodies as in Figures 1(B) and 2(A),(C), and (D), assumed to originate from the ER., were also observed in some parts of the cytoplasm. A densely staining amorphous material occurred both within and outside the tubules. Vesicles containing densely staining amorphous material or without any apparent contents were occasionally seen within these aggregates but were more common around the periphery, Figure 1(B). These tubular aggregates persisted through the remaining stages of the infection, but the vesicles with fibrils decreased markedly in number as the infection progressed, Figure 2(A) and (B). Remnant vesicles usually occurred singly, and many were still enclosed in the second membrane.
Inductively coupled plasma mass spectrometry
I. Amai, M. Kojima, Cytopathic alterations of barley root tissues infected with barley yellow dwarf virus, Bulletin of the Faculty of Agriculture, Niigata University (42) (1990) 25-33.
G. Zhai, KS. Walters, D.W. Peate, P.J. Alvarez, J.L. Schnoor, Transport of gold nanoparticles through plasmodesmata and precipitation of gold ions in woody poplar, Environmental science & technology letters 1(2) (2014) 146-151.