Main sources of scatter in mechanical fatigue.
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These books synthesize perspectives of renowned scientists from the world’s most prestigious institutions - from Fukushima Renewable Energy Institute in Japan to Stanford University in the United States, including Columbia University (US), University of Sidney (AU), University of Miami (USA), Cardiff University (UK), and many others.
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IntechOpen and Knowledge Unlatched formed a partnership to support researchers working in engineering sciences by enabling an easier approach to publishing Open Access content. Using the Knowledge Unlatched crowdfunding model to raise the publishing costs through libraries around the world, Open Access Publishing Fee (OAPF) was not required from the authors.
\n\nInitially, the partnership supported engineering research, but it soon grew to include physical and life sciences, attracting more researchers to the advantages of Open Access publishing.
\n\n\n\nThese books synthesize perspectives of renowned scientists from the world’s most prestigious institutions - from Fukushima Renewable Energy Institute in Japan to Stanford University in the United States, including Columbia University (US), University of Sidney (AU), University of Miami (USA), Cardiff University (UK), and many others.
\n\nThis collaboration embodied the true essence of Open Access by simplifying the approach to OA publishing for Academic editors and authors who contributed their research and allowed the new research to be made available free and open to anyone anywhere in the world.
\n\nTo celebrate the 50 books published, we have gathered them at one location - just one click away, so that you can easily browse the subjects of your interest, download the content directly, share it or read online.
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Shishkovsky",coverURL:"https://cdn.intechopen.com/books/images_new/6306.jpg",licenceType:"CC BY 3.0",editedByType:"Edited by",editors:[{id:"178616",title:"Prof.",name:"Igor",middleName:"V.",surname:"Shishkovsky",slug:"igor-shishkovsky",fullName:"Igor Shishkovsky"}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"}}},ofsBook:{item:{type:"book",id:"11285",leadTitle:null,title:"Spinal Cord Injury - Current Trends in Acute Management, Function Preservation and Rehabilitation Protocols",subtitle:null,reviewType:"peer-reviewed",abstract:"
\r\n\tSpinal cord injury represents a relatively frequent clinical scenario that emergency doctors, neuroradiologists, and spine surgeons have to deal with in their daily practice.
\r\n\r\n\tAlthough there are many publications on this topic, a consensus on the preferred management has not been reached yet. In fact, other than clearly surgical or non-surgical patients, there is a non-negligible number of cases where an interdisciplinary discussion is strictly needed, eventually determining a case-by-case treatment selection.
\r\n\r\n\tEmergency decompression surgery, often associated with fusion, represents an effective treatment for critical compressions of the spinal cord, while its role in subacute cases is still debated. Different medical managements have been proposed for the acute, subacute, and chronic phases, respectively. Since function preservation is the primary outcome to be pursued, the multidisciplinary case discussion is a fundamental step in the decision-making process. However, a practical guide on the state of the art on spinal cord injury management may result as useful to a large audience of practitioners.
",isbn:"978-1-80355-877-6",printIsbn:"978-1-80355-876-9",pdfIsbn:"978-1-80355-878-3",doi:null,price:0,priceEur:0,priceUsd:0,slug:null,numberOfPages:0,isOpenForSubmission:!1,isSalesforceBook:!1,hash:"fc1ece21c6d20adecf2b9fe16489a07d",bookSignature:"Dr. Luca Ricciardi, Dr. Giorgio Lofrese, Dr. Andrea Perna and Ph.D. Sokol Trungu",publishedDate:null,coverURL:"https://cdn.intechopen.com/books/images_new/11285.jpg",keywords:"Concussion, Medullary Edema, Paraplegia, Neurotrauma, Functional Impairment, Neurorehabilitation, Physiotherapy, Steroids, Rhiluzole, Arthrodesis, Fusion, Instrumentation",numberOfDownloads:16,numberOfWosCitations:0,numberOfCrossrefCitations:0,numberOfDimensionsCitations:0,numberOfTotalCitations:0,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"November 4th 2021",dateEndSecondStepPublish:"February 24th 2022",dateEndThirdStepPublish:"April 25th 2022",dateEndFourthStepPublish:"July 14th 2022",dateEndFifthStepPublish:"September 12th 2022",remainingDaysToSecondStep:"3 months",secondStepPassed:!0,currentStepOfPublishingProcess:4,editedByType:null,kuFlag:!1,biosketch:"The clinical researcher focused on spine and spinal cord disorders. Dr. Ricciardi serves as a guest editor, editorial board member, and reviewer of many indexed journals such as the Journal of Neurosurgical Sciences and Frontiers in Neurooncology, Life, and Cell. He is a co-chairman for SPINE20, the World Congress on Spine Disorders at the G20 conference in Rome, Italy, and an individual delegate at the European Association of Neurosurgical Societies.",coeditorOneBiosketch:"A neurosurgeon specialized in craniocervical junction diseases and minimally invasive spine surgery. Dr. Lofrese was awarded the European Young Researcher Award (AOSpine) and the Young Neurosurgeon Award (WFNS). He is a member of EUROSPINE, SPINE20, and the European Association of Neurosurgical Societies.",coeditorTwoBiosketch:"Dr. Perna's main fields of study are the pathologies of the spine, with particular attention to spinal deformities, traumatological surgery, and infectious pathologies such as spondylodiscitis. His publications give particular attention to lateral surgery, while his other fields of interest are hand surgery and biomechanics applied to orthopedics.",coeditorThreeBiosketch:"Dr. Trungu completed his Ph.D. in Neuroscience and Neurosurgery at Sapienza University of Rome, Italy. His areas of special interest are Minimally Invasive Spine Surgery (MISS), complex spine surgery, spine trauma focusing on acute spinal cord injury, primary and secondary spinal tumors, and Neuro-oncology.",coeditorFourBiosketch:null,coeditorFiveBiosketch:null,editors:[{id:"421212",title:"Dr.",name:"Luca",middleName:null,surname:"Ricciardi",slug:"luca-ricciardi",fullName:"Luca Ricciardi",profilePictureURL:"https://mts.intechopen.com/storage/users/421212/images/system/421212.jpg",biography:"Dr. Ricciardi graduated in Medicine and Surgery in 2013 and finished his residency in Neurosurgery in 2019.\nIn 2017, he completed a fellowship in spinal deformities at the Catholic University of Rome - Italy, and in 2018 he completed a research fellowship at the Mayo Clinic, Jacksonville, Florida, US. He also completed the four-year training course of the European Association of Neurosurgical Societies and completed the European Board Exam part-1 for FEBNS.\nDr. Ricciardi has authored more than 60 papers published in peer-reviewed international journals. He has been serving as a reviewer for more than 15 scientific journals. He has been awarded as Publons Academy Mentor for training in peer-review, and he has conducted more than 80 certified peer reviews by the date. ( https://publons.com/researcher/1705851/luca-ricciardi/ ) \nIn 2021, Dr. Ricciardi was invited as Guest Editor for Systematic Reviews and Meta-analyses on the Journal of Neurosurgical Sciences, and as Invited Editor on Frontiers in Neurooncology, Life, and Cell. \nIn 2021, Dr. Ricciardi has been nominated co-Chairman and President of the Scientific Committee at SPINE20, the World Congress on Spine Disorders at the G20 conference in Rome, Italy.",institutionString:"Sapienza University of Rome",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"0",totalChapterViews:"0",totalEditedBooks:"0",institution:{name:"Sapienza University of Rome",institutionURL:null,country:{name:"Italy"}}}],coeditorOne:{id:"436982",title:"Dr.",name:"Giorgio",middleName:null,surname:"Lofrese",slug:"giorgio-lofrese",fullName:"Giorgio Lofrese",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y00003FVMDgQAP/Profile_Picture_1633074659823",biography:"Dr. Lofrese is a neurosurgeon specialized in cranio-cervical junction diseases and minimally invasive spine surgery. As a former resident of the Catholic University in Rome, he completed his training in Milan, Bologna, and New York, concluding it in Curitiba with an AOSpine clinical fellowship. He is currently a permanent neurosurgeon at the Bufalini Hospital in Cesena, and a consultant neurosurgeon at the State Hospital of the Republic of San Marino. He was awarded with the European Young Researcher Award (AOSpine) and the Young Neurosurgeon Award (WFNS). 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He also has numerous scientific publications relating to spinal deformity correction surgery and minimally invasive surgery, with particular attention to lateral surgery. His other fields of interest are hand surgery and biomechanics applied to orthopedics.",institutionString:"Gemelli Hospital",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"0",totalChapterViews:"0",totalEditedBooks:"0",institution:null},coeditorThree:{id:"440065",title:"Ph.D.",name:"Sokol",middleName:null,surname:"Trungu",slug:"sokol-trungu",fullName:"Sokol Trungu",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y00003FYl9aQAD/Profile_Picture_1633512850348",biography:"Dr. Trungu graduated with honors in Medicine and Surgery in 2010 and finished his residency in Neurosurgery in 2017. In 2021, he completed his PhD in Neuroscience and Neurosurgery at the Sapienza University of Rome, Italy. Currently, he is working as a consultant neurosurgeon at Cardinale G. Panico Hospital, Tricase, Italy. His areas of special interest are Minimally Invasive Spine Surgery (MISS), complex spine surgery, spine trauma focusing in acute spinal cord injury, primary and secondary spinal tumors, Neurovascular and Neuro-oncology. 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From chapter submission and review, to approval and revision, copyediting and design, until final publication, I work closely with authors and editors to ensure a simple and easy publishing process. I maintain constant and effective communication with authors, editors and reviewers, which allows for a level of personal support that enables contributors to fully commit and concentrate on the chapters they are writing, editing, or reviewing. I assist authors in the preparation of their full chapter submissions and track important deadlines and ensure they are met. I help to coordinate internal processes such as linguistic review, and monitor the technical aspects of the process. As an ASM I am also involved in the acquisition of editors. 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Venkateswarlu",coverURL:"https://cdn.intechopen.com/books/images_new/371.jpg",editedByType:"Edited by",editors:[{id:"58592",title:"Dr.",name:"Arun",surname:"Shanker",slug:"arun-shanker",fullName:"Arun Shanker"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"72",title:"Ionic Liquids",subtitle:"Theory, Properties, New Approaches",isOpenForSubmission:!1,hash:"d94ffa3cfa10505e3b1d676d46fcd3f5",slug:"ionic-liquids-theory-properties-new-approaches",bookSignature:"Alexander Kokorin",coverURL:"https://cdn.intechopen.com/books/images_new/72.jpg",editedByType:"Edited by",editors:[{id:"19816",title:"Prof.",name:"Alexander",surname:"Kokorin",slug:"alexander-kokorin",fullName:"Alexander Kokorin"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"314",title:"Regenerative Medicine and Tissue Engineering",subtitle:"Cells and Biomaterials",isOpenForSubmission:!1,hash:"bb67e80e480c86bb8315458012d65686",slug:"regenerative-medicine-and-tissue-engineering-cells-and-biomaterials",bookSignature:"Daniel Eberli",coverURL:"https://cdn.intechopen.com/books/images_new/314.jpg",editedByType:"Edited by",editors:[{id:"6495",title:"Dr.",name:"Daniel",surname:"Eberli",slug:"daniel-eberli",fullName:"Daniel Eberli"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"57",title:"Physics and Applications of Graphene",subtitle:"Experiments",isOpenForSubmission:!1,hash:"0e6622a71cf4f02f45bfdd5691e1189a",slug:"physics-and-applications-of-graphene-experiments",bookSignature:"Sergey Mikhailov",coverURL:"https://cdn.intechopen.com/books/images_new/57.jpg",editedByType:"Edited by",editors:[{id:"16042",title:"Dr.",name:"Sergey",surname:"Mikhailov",slug:"sergey-mikhailov",fullName:"Sergey Mikhailov"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"1373",title:"Ionic Liquids",subtitle:"Applications and Perspectives",isOpenForSubmission:!1,hash:"5e9ae5ae9167cde4b344e499a792c41c",slug:"ionic-liquids-applications-and-perspectives",bookSignature:"Alexander Kokorin",coverURL:"https://cdn.intechopen.com/books/images_new/1373.jpg",editedByType:"Edited by",editors:[{id:"19816",title:"Prof.",name:"Alexander",surname:"Kokorin",slug:"alexander-kokorin",fullName:"Alexander Kokorin"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}}]},chapter:{item:{type:"chapter",id:"72074",title:"The Chemistry Behind Plant DNA Isolation Protocols",doi:"10.5772/intechopen.92206",slug:"the-chemistry-behind-plant-dna-isolation-protocols",body:'The isolation of good-quality DNA is the prerequisite for molecular research. Maintaining yield and quality of DNA during plant DNA extraction is one of the difficult tasks compared to that of animals, because of its rigid cell wall, which is made up of cellulose along with other variable levels of chemical components such as polysaccharides, polyphenols, proteins, and lipids that act as a contaminant during DNA extraction. The amount of these components varies according to plant species, plant part used, environmental condition, and growth stage and it is very problematic when isolating DNA. For example, cereals are rich in carbohydrates whereas medicinal plants are rich in the polyphenols wherein stressed plants have higher polyphenols. These contaminants can be removed during extraction by standardizing basic DNA extraction protocol [1, 2, 3].
Generally fresh leaves aged 15–20 days are preferred for plant tissues (fresh, freeze-dried, or frozen in liquid nitrogen) and usually ruptured by mechanical force in pestle and motor or TissueLyser. If liquid nitrogen is unavailable, CTAB buffer can be used directly or prewarmed for grinding. The main objective of various DNA isolation methods is development of relatively quick, inexpensive, and consistent protocol to extract high-quality DNA with better yield. Generally, leaf samples contain large quantities of polyphenols, tannins, and polysaccharides. The basic principle of DNA isolation is disruption of the cell wall, cell membrane, and nuclear membrane to release the highly intact DNA into solution followed by precipitation of DNA and removal of the contaminating biomolecules such as the proteins, polysaccharides, lipids, phenols, and other secondary metabolites by enzymatic or chemical methods [4].
The plant DNA is extracted by either CTAB-based [5, 6] or sodium dodecyl sulfate (SDS)-based methods [7]. The majority of the protocols developed for DNA extraction are modified versions of hexadecyltrimethylammonium bromide (CTAB) extraction [8]. The role of various chemicals involved in CTAB extraction method has been described in the present communication.
The CTAB buffer mainly includes CTAB, sodium chloride (NaCl), and ethylenediaminetetraacetic acid (EDTA) Tris2-amino-2-hydroxymethyl-1,3-propanediol (TRIS), polyvinylpyrrolidone (PVP), and β mercaptoethanol.
The plant cells enclose themselves in complex polysaccharide cell wall, of which cellulose is a major constituent [9], which is crystalline in nature, due to chain-like structure and intermolecular hydrogen bonding. This can be weakened to open the cell wall, by applying mechanical force exerted during grinding along with CTAB buffer or liquid nitrogen.
Cell membrane lies next to the cell wall and cellulose and is composed of a diverse set of phospholipid molecules and proteins. It dissolves in surfactant, detergents, which are amphipathic (hydrophobic tail and hydrophilic head) in nature, very much similar to phospholipid membranes. Surfactants are characterized based on their hydrophilic group, that is, ionic, nonionic, and zwitterionic. Ionic surfactant has been always better in denaturing protein molecules, and thus in dissolving the membranes [10].
CTAB, a cationic detergent, constitutes a long hydrophobic hydrocarbon chain and a hydrophilic head. It forms micelle in water because of the amphipathic nature. During DNA extraction, under aqueous condition, CTAB comes in contact with the biological membrane, captures the lipids (Figure 1), and results in the release of nucleus, which is devoid of membrane [11]. Plant tissue, which is rich in complex polysaccharides and secondary metabolites, interfere and co-precipitate with DNA; CTAB along with some other chemicals like PVP is used to minimize the effect of these metabolites.
CTAB’s role in removing membrane [
CTAB works differently based on the ionic strength of the solution. At a low ionic strength, it precipitates nucleic acid and acidic polysaccharides (pectin, xylan, and carrageenan), while protein and neutral polysaccharides (dextran, gum locust bean, starch, and inulin) remain in the solution [12]. However, at high ionic concentration, it gets bound to the polysaccharides and forms complexes that are removed during subsequent chloroform extraction. It also denatures or inhibits the activity of proteins and/or enzymes [13].
NaCl helps to remove proteins that are bound to the DNA. It also helps to keep the proteins dissolved in the aqueous layer so they do not precipitate in the alcohol along with the DNA by neutralizing the negative charges on the DNA so that the molecules can come together.
Osmosis occurs when cell is subjected to hypo or hypertonic solution. If the cells are kept in hypotonic solution, water enters inside the cell that leads to swelling, rising internal pressure and eventually bursting. On the other hand, in a hypertonic solution, water tends to ooze out from the cell and eventually plant cell shrinks and crumples, which leads to plasmolysis. Therefore, salt concentration plays a significant role in cell lysis.
The salt concentration of more than 0.5 M provides the ionic strength needed for CTAB to precipitate polysaccharides [8, 14]. In several protocols, 1.4 M concentration of NaCl has been suggested; however, in the protocols developed for getting rid of polysaccharides, higher concentration of the NaCl and/or CTAB has been recommended.
Tris is a (hydroxymethyl) aminomethane with the molecular formula (HOCH2)3CNH2, which has three primary alcohols and an amine group with a pKa of 8.1, is an effective buffer between pH 7 and 9. When the pH is adjusted to 8, with HCl, it contains a mixture of weak base and its conjugate weak acid (Figure 2), which can act as a buffer and further increases the permeability of the cell wall. When the cell wall and membranes are broken during tissue grinding, compartmentalization ends, cytoplasmic material is released, because of which the pH gets altered, and consequently the stability of biomolecules like nucleic acid is disturbed. The buffer plays a major role under such situations, and the Tris buffer maintains the pH of the solution.
Tris buffer after titration of Tris base solution [
EDTA (C10H16N2O8) chelates divalent cations, such as Mg2+ and Ca2+ (Figure 3), which is present in the enzymes and reduces the enzyme activity of DNase and RNase. Divalent cations are the cofactors for many enzymes that increase the activity of the enzyme. For example, DNase enzyme requires Mg2+ ions as a cofactor for its activity. Chelating Mg2+ ions with EDTA makes enzyme DNase nonfunctional, and thereby protects the DNA. The Mg2+ ions are also required for aggregation of nucleic acid with protein; whereas Ca2+ ions are required for cementing of cell wall’s middle layer and membrane stability. Thus, harnessing them by EDTA results in destabilization of the enzyme’s integrity.
EDTA chelates divalent cations like magnesium and calcium [
Plants are rich in phenolics compounds and to get a quality DNA these should be removed. β-Mercaptoethanol (HOCH2CH2SH) is added most of the time in extraction buffers and is a strong reducing agent to clean tannins and other polyphenols present in the crude plant extract.
Globular proteins get dissolved in water. To make them insoluble, their denaturation is one of the alternatives that can be done at tertiary and quaternary structure level of protein by reducing intermolecular disulfide linkages. β-Mercaptoethanol reduces disulfide bonds of the protein (Figure 4) and thus the proteins are denatured.
β-Mercaptoethanol reduces disulfide linkage of protein, thus denaturing it [
PVP is added to remove phenolic compounds from plant DNA extracts. Polyphenol is a major component in medicinal plants, woody plants, and mature plant parts. It is present in the vacuole, while its oxidizing enzyme, polyphenol oxidase (PPO) is located in plastid [15]. During grinding of the tissue, compartmentalization breaks and PPO convert polyphenols into quinone, which gives brown coloration. Polyphenols bind DNA and make downstream processing difficult as they get co-precipitated with the nucleic acid. PVP removes polyphenolic contamination by binding it through hydrogen bond [16, 17]. Thus, it prevents polyphenol oxidation, and thereby browning of DNA samples [18]. When the extract is centrifuged with chloroform, PVP complexes get accumulated at the interphase.
Cell lysate mixture with CTAB buffer should be kept in the water bath at 65°C, which irreversibly inhibits enzyme DNase. After removing the sample from water bath, it should be allowed to cool at room temperature, then chloroform:isoamyl alcohol (24:1) or phenol:chloroform:isoamyl alcohol (25:24:1) shall be added. Chloroform:octanol (24:1) can also be used instead of chloroform:isoamyl alcohol (24:1).
Phenol is an organic solvent, so it is not miscible with water and is used along with chloroform and isoamyl alcohol for purification of the DNA to remove proteins and polysaccharide contaminants. When phenol is shaken with cell extract, the nonpolar components of the cell will be fractionated in phenol, leaving polar ones in water. DNA is insoluble in phenol because phenol is a nonpolar solution. On the other side, protein has both polar and nonpolar groups present in it because of the long chain of different amino acids. Different amino acids have different groups present on their side chain. Also, the folding of the protein into the secondary, tertiary, and quaternary structure depends on the polarity of the amino acids. The bonds between amino acids are broken by the addition of phenol and protein gets denatured and ultimately the protein becomes unfolded.
Centrifugation after phenol:chloroform:isoamyl alcohol in 25:24:1 ratio steps gives three layers, that is aqueous, interphase, and at bottom organic phase. At neutral to alkaline pH, the nucleic acids are negatively charged and polar. Therefore, it is hydrophilic and remains in an aqueous phase. In aqueous solution, hydrophobic amino acid forms a protective core. However, after denaturation, nonpolar cores (hydrophobic) get exposed, causing precipitation of protein as well as some polysaccharides at interphase.
The phenol-chloroform combination reduces the partitioning of poly (A) and mRNA into the organic phase and reduces the formation of insoluble RNA protein complexes at the interphase. Phenol retains about 10–15% of the aqueous phase, which results in a similar loss of RNA; chloroform prevents this retention of water and thus improves yields.
Only neutral phenol should be used, as acidic phenol dissolves DNA within, or phenol turns into quinones by oxidation and it forms free radical, degrading nucleic acid. Simple observation of phenol’s pink color will state its acidic nature. The centrifugation after chloroform:isoamyl alcohol step should be done under room temperature, because below 15°C, CTAB/nucleic acid forms irreversible aggregates and may precipitate. During this step, the DNA shall be in aqueous phase [19].
Chloroform (CHCl3) or trichloromethane is a nonpolar (hydrophobic) solvent, in which nonpolar proteins and lipids get dissolved to promote the partitioning of lipids and cellular debris into the organic phase, leaving isolated DNA protected in the aqueous phase. Chloroform ensures phase separation of the two liquids because it has a higher density (1.47 g/cm3) and forces a sharper separation of the organic and aqueous phases, thereby assisting in the removal of the aqueous phase with minimal cross contamination from the organic phase. As chloroform is volatile in nature, it does not hinder the downstream process.
Chloroform comes in contact with the air and forms gas phosgene (COCl2, carbonyl chloride), which is harmful. If we simply use chloroform only, the gas entrapment causes foaming or frothing, it foams up between interphase during extraction process and makes it difficult to properly purify the DNA, which is prevented when chloroform is used along with isoamyl alcohol or isopentanol {(CH3)2CHCH2CH2OH} or octanol {CH3(CH2)7OH} by preventing the emulsification of a solution. Isoamyl alcohol or isopentanol is not miscible in the aqueous solution because it is a long-chain aliphatic compound, containing five carbon atoms and stabilizes the interphase between organic and aqueous layer. The aqueous phase contains DNA and the organic phase contains lipid, proteins, and other impurities. Isoamyl alcohol helps to inhibit RNase activity and to help prevent the solubilization in the phenol phase of long RNA molecules with long poly (A) portions. This will increase the purity of DNA.
Genomic DNA should be treated with Ribonuclease A (RNase A) to remove the contamination of RNA for DNA purification. RNase A is an endoribonuclease that catalyzes the hydrolysis of the 3′,5′-phosphodiester linkage of RNA at the 5′-ester bond in a two-step reaction. The first step is a transphosphorylation to give an oligonucleotide terminating in a pyrimidine 2′,3′-cyclic phosphate. The second is the hydrolysis of the cyclic phosphate to give a terminal 3′-phosphate. Numerous chemical studies have suggested that histidine 12, histidine 119, and lysine 41 are involved in the active site of the enzyme and the DNA is devoid of 2′OH group (deoxy), it remains secure (Figures 5 and 6) [20].
(A) The hydrolysis reaction catalyzed by RNase A. An RNA molecule is a chain of nucleotides linked by the phosphodiester bond, which may be cleaved by RNase [
The catalytic mechanism of RNase A, which contains two critical residues: His-12 and His-119 [
Alcohol is used to precipitate the DNA out of the extraction solution, so we can wash all those salts and chemicals away and then dissolve it in our final solvent—usually water or some variant of Tris-EDTA solution. DNA remains dissolved in aqueous solution because DNA has phosphodiester backbone, which is hydrophilic in nature. Water molecule forms hydration shell around DNA by forming hydrogen bonds. Isopropanol/ethanol is used in precipitation of DNA, which breaks the hydration shell. Isopropanol is a good choice for precipitation of DNA. The amount of isopropanol requirement is less (0.6–0.7 volume of supernatant), as isopropanol has a higher capacity to reduce the dielectric constant of water than the ethanol (2–3 volume) and also requires a fair amount of salt to work. RNA which has extra 2′OH remains hydrogen bounded with water more strongly than DNA tends to stay soluble in it, thus selective precipitation of DNA can be done. Isopropanol also dissolves nonpolar solvents such as chloroform, thus the impurities form previous step can also be removed.
Using ice-cold isopropanol is generally practiced, but many researchers say that it should be used at room temperature, otherwise it will precipitate polysaccharides also [21]. Though the yield of DNA will be increased at low temperature, it may increase impurities [22].
The role of the salt in the extraction protocol is to neutralize the charges on the sugar phosphate backbone of the DNA. Sodium acetate with pH 5.2 is commonly used for precipitation of nucleic acid along with ethanol [23]. In solution, sodium acetate dissociates into Na+ and [CH3COO]−. The positively charged sodium ions neutralize the negative charge on the PO3− groups on the sugar phosphate backbone of nucleic acids reducing repulsion between DNA molecules, making the DNA molecule far less hydrophilic, and therefore much less soluble in water. The electrostatic attraction between the Na+ ions in solution and the PO3− ions on the nucleic acid are dictated by Coulomb’s Law, which is affected by the dielectric constant of the solution. Water has a high dielectric constant, which makes it fairly difficult for the Na+ and PO3− to come together. This is useful in aggregation and formation of tangled mass. It is also called as salting out. Nevertheless, it is not seen when salt alone is used. It requires the solution with low dielectric constant, which allows this interaction. This is affected by either ethanol or isopropanol, which has a much lower dielectric constant, making it much easier for Na+ to interact with the PO3−, shield its charge, and make the nucleic acid less hydrophilic, causing the DNA to drop out of solution (Figure 7).
Role of salt in DNA precipitation [
DNA precipitate is washed again with 70% ethanol to rinse excess salt that might come along with the extraction buffers from the pellet [24], centrifuged, and ethanol is discarded, leaving DNA in the precipitate. Precipitate is air-dried or vacuum-dried. Over drying should be avoided as DNA converts B form to D form, which is difficult to dissolve later [25].
In older times in DNA isolation methods, DNA used to be stored dry and diluted when required. Nowadays, for long-term storage, it is prudent to store DNA in a buffer that maintains its pH and keeps it from getting degraded. TE buffer contains Tris (10 mM) and EDTA (1 mM), where Tris is the buffering component and EDTA the chelating component. For DNA isolation, the pH is usually set to 7.5–8.5, the slight alkalinity of TE buffer also prevents chances of acid hydrolysis that may further disrupt the stability of DNA stored in water. Tris amino constituent of TE buffer has the ability to protect DNA strands from radiation damage, in both solid state and fluid solution. As radiation produces free radicals, it may break DNA strands. Thus, in the fluid solution at ambient temperature Tris acts by scavenging hydroxyl radicals [26]. The purpose of EDTA is to chelate Mg2+ ions in solution necessary for DNase or RNase action, thus protecting the DNA from DNases or RNase.
Sterile water can be utilized for short-duration storage of DNA. If TE buffer is used for storage of DNA, it should be diluted further with sterile water to dilute EDTA concentration for making magnesium ions available for polymerase activity during PCR because if DNA has to be sent for sequencing afterward, the buffer components in TE hinders the process. The same EDTA that chelates ions to degrade magnesium also hinders the action of DNA polymerases during PCR, which can be overcome by adding more magnesium to the master mix, or perhaps diluting the DNA sample so that the already low concentrations of EDTA do not actually disrupt PCR. In fact, in a large number of cases, they do not.
To release new or optimized component, it is mandatory to evaluate it under fatigue load conditions to prevent any kind of unexpected failure on the product life. To reduce the time of the development process, accelerated tests can be performed to obtain the mechanical strength feedback to improve its fatigue performance, thereby reducing the excessive material or reinforcing critical areas as stress concentrators. Nowadays, this information directly influences the component with physical optimization or analyzes it in a virtual way, in order to reduce the number of physical prototypes. The importance of implementing accelerated tests in the early stages of design is to evaluate components developed in the concept stage or to modify it, for changes in design during its production life act as facelift. Figure 1 shows the time development reduction when accelerated tests are implemented. Most improvements have to be made prior to mass production and in the early manufacturing process of the tooling [1].
Development time reduction using accelerated tests.
To perform this kind of test, it is necessary to evaluate the critical failures on the component related with the major probability of occurrence. All the load cases are evaluated; however, the target of this kind of a test is to evaluate the component in an easier way, with uniaxial test, where it is possible.
In other cases, the test is developed depending on the part or the process that has to be evaluated, for example, a new stamped part, or the weld cordon or the sequence of the welding. In those cases, a localized damage is developed with a correlation to its use in normal load conditions, but the important thing is to find the direction and load amplitude generated by use conditions, to get a correlation between the number of load repetitions and how many kilometers or time of use represent it.
The way to develop this kind of test starts with the instrumentation of a car with displacement transducers, accelerometers, force-moment transducers and strain gauges where it is necessary. The instrumented car is measured on different roads, used by different drivers in all the markets and under different weather conditions to acquire loads to measure the changes on the responses of the wheels.
These responses are acquired as signals, which are analyzed to synthetize it in one signal representing all of these driving and use conditions. The new signal used for the durability test is known as spectrum. The reproduction of this spectrum in labs reaches the same damage on the component as in the roads, but the target of this kind of test is to reduce the time of evaluation in a controlled manner to detect the location of failure, the moment of occurrence and its propagation. To accelerate the test, the spectrum is extrapolated and proving grounds are developed. These are faster than duration cars on the roads, but it is possible to reduce the evaluation time developing accelerated test on test benches through extrapolating the loads; to perform this, the component can be mounted as assembly, subassembly or component as itself. Test reduction is reached due to the loads that represent more damage by their amplitude and severity, than that applied on the component in normal-use conditions.
Test responses and desired signals have to be evaluated through statistical analysis. The correlation of results in lab and on roads is essential as the main target of accelerated tests is to reproduce the same failures as on the road so as to take steps to prevent them. The component´s load spectrum is made more aggressive by including all the variables in load conditions as in the case of drivers on roads, and the spectrum is also modified to build test requirements to include the safety factor.
In this chapter, a review of durability test has been performed, describing the process to develop a fatigue test and also the development of accelerated test. A general overview is done on the product evolution process (PEP) to define where the evaluation of the component is applied and how it affects the development process, the general process of the fatigue life evaluation of the component, a description of the finite element analysis and its application on fatigue life prediction to evaluate an automotive component and develop stiffness devices necessary for the test that are used with the modified loads to reproduce the failures.
It is important to evaluate components in experimental tests because fatigue strength has its inherent scatter due to four main factors: the loading, design, manufacturing and material (Figure 2). Experimental results under variable loads differ from analytical predictions owing to the effect of sequence loads [2, 3], Jimenez et al. [4] proposed a modification in Linear Damage Rule to include the effect of sequence in fatigue life prediction.
Parameters influencing the structural durability of components.
While manufacturing generally determines the strength and scatter, the geometry can modify the effect of mechanical properties [5, 6] due to the material that has variations on its properties. Loads have the major variability due to the diversity of drivers and factors such as number of passengers, weight on the car and its distribution, weather and its effects on the interchange of the loads between the non-suspended mass and the pave and the loads generated by bumpy ways and maneuvers.
Fatigue strength at the endurance limit is affected by the type of load and the size, reliability and surface roughness of the component [7]. The surface roughness can be improved with processes such as shot peening, which is important because fatigue cracks usually initiate at the surface in homogeneous materials [8, 9].
In durability tests, the aim is to minimize the likelihood of failure applied for the more aggressive driver using the weakest component. Figure 3 shows a strength-load interference model [10], which helps to manage the likelihood of failure of a component. As described in Figure 2, the component has different sources of scatter and its structural strength is determined on a bell-shaped curve. On the other side is evaluated the scatter for the loads applied to the component. The safety factor is defined by the difference between the central value of applied loads and its difference with the central value of the component\'s structural strength.
Failure likelihood in components subjected to cyclic loads.
Jimenez et al. [7] reported that the advantage of component testing is that the effects of the material, manufacturing process and geometry are inherently accounted for. Although with controlled process as in test laboratory, fatigue test results have scatter, the main sources of scatter are summarized in Table 1 [11].
Variable | Fatigue in components | |
---|---|---|
In laboratory | In service | |
Production and materials. | Production samples and different lots | Material from different lots and suppliers processed in different facilities. |
Quality on the specimen Surface. | Quality on Surface in critical areas as in notches. | |
Loads including environment | Type of load (CA, VA)* | Loads in service from different users |
Accuracy of test equipment | Residual fatigue life | |
Environment | Temperature, humidity in laboratory | Temperature, snow, rain. |
Human | Skills and expertise of lab staff to perform and evaluate the test. | Different users and styles of use, overloads not expected, abuse loads. Responses changed for the environment. |
Main sources of scatter in mechanical fatigue.
CA-Constant amplitude; VA-Variable Amplitude.
Fatigue evaluation is not simple to predict by analytical methods, and to perform durability assessment and to predict the component´s life, it is necessary to measure the most precise information, and to do this, the loads in service are acquired and analyzed, to reproduce them as shown in Figure 4.
Parameters influencing the structural durability of components.
To build a track to perform a durability test, it is necessary to get information from the customers through a data acquisition with strain gauges, accelerometers and displacement transducers; then this information is analyzed. The output of this analysis is to get the desired signal that is known as spectrum. The importance of getting the spectrum is to compare the loads with the S-N curve in order to predict the component life through damage accumulated rule. Every step of the development process is evaluated to improve its mechanical response, and after the design is released, tests are performed to monitor the quality of the product to prevent failures in its service life.
To reduce the time required for testing on public roads, accelerated tests are performed on proving grounds. This simulates road damage for different maneuvers, different vertical loads of frames and different longitudinal dynamics for accelerating and braking, lateral dynamics and vibrations [12], combining all the events (normal roads, rough roads, emergency braking, high speed, city and country roads). In addition, the tests can be performed in the laboratory [5]. It is possible to increase the number of repetitions at high or medium loads, avoiding inadmissible stresses that satisfy the test results. Although some proposals [13] have included the omission of low loads, they depend on the type of material and the application (Figure 5). The main objective is to develop an accelerated spectrum to get a test track.
Schematic S-N curve: (a) linear-linear, (b) semi-log, and (c) log–log.
The loads acquired are compared with S-N curves. The S-N curves are often expressed on semi-log, normal and log–log coordinates. Figure 5 shows a schematic curve on different coordinates, linear, semi-log and log–log. The most common representation is log-log since it becomes linear (Figure 5c).
The S-N curve represents the material or component fatigue strength, and is split into regions depending on its cycles. Extremely low cycle fatigue (ELCF) is defined from 0 until 100 cycles, between this limit and until 1000 cycles is low cycle fatigue (LCF), and between 1000 cycles and until 1 × 106 for steel and 5 × 107 for nodular cast iron is defined as high cycle fatigue (HCF). Anything beyond this point is defined as very high cycle fatigue (VHCF) [7].
To compare the S-N curve with loads, the time history is analyzed. Figure 6 shows a schematic waveform. The main characteristic is the stress amplitude Sa. If it has constant amplitude, the stress range SR is constant and is defined by the difference of the maximum stress (Smax) and minimum (Smin) in a cycle (Eqs. (1)–(3)).
Signal characteristics.
The mean stress Sm is defined as
A fully alternating stress Sm = Sa.
The stress ratio
The fatigue damage of a component is influenced in high cycle region by the mean stress expressed by its stress ratio. In normal
Stress ratio.
The amplitude ratio is the ratio of the stress amplitude to mean stress as show in Eq. (5).
The loads are monitored with cycle counting that is used to summarize variable amplitude time histories, providing the repetitions of the load during the time history. There are different cycle counting methods such as the Rainflow used to extract cycles from random histories in the time domain [13, 14], based on the analogy of raindrops falling on a roof. Figure 8 shows Rainflow counting process.
Rainflow counting process: (a) initial counting, (b) continue counting, and (c) residue.
The cycle counting is represented in a matrix based on Figure 5. The signal has 2 cycles from 5 to 3, 1 cycle from 6 to 3, 1 cycle from 1 to 5, 1 cycle from 2 to 4 (Figure 5a), 2 cycles from 1 to 6 (Figure 5b), and it has residue. In Figure 5c, these cycles are tabulated on a matrix, which depending on its counting can be represented by colors.
It is possible to evaluate time histories with other types of cycle counting methods, such as the level crossing method [15] where the amplitudes of the loads are split into a number of levels based on ranges, and the load is counted when it has peak at a different level, changing its slope from positive to negative or negative to positive; the cycle counting is shown in Figure 9.
Level crossing counting process: (a) time history, (b) quantity per class, (c) histogram, and (d) absolute cumulative frequency.
In the range pair counting method, the magnitude of loads is split into a number of levels. The result of the extracted number of reversals is shown tabulated in Figure 10b. Table 2 summarizes the events counted in Figure 10.
Range pair counting process, (a) time signal and (b) events.
Range (Units) | Cycle counts | Events |
---|---|---|
8 | 1.0 | G-H,H-I |
7 | 0.5 | C-D |
6 | 1.5 | F-G, I-J,K-L |
5 | 1.0 | D-E,L-M |
4 | 1.0 | B-C,J-K |
3 | 1.0 | A-B,E-F |
2 | 0 | |
1 | 0 |
Range pair counting.
The signals can be seen in time domain and frequency domain. A transfer function can be used in the frequency domain to relate the power spectral density (PSD) of the input desired load to the PSD of the output stress (Eq. (6)) [16]:
here the squaring process is required to get the transfer function in the correct units of PSD stress [17]. In this equation, σPSD (w) is the PSD of the stress at frequency
The time histories for constant amplitude test spectrum is linear (Figure 11a), and for variable amplitude, it is a curve (Figure 11b) generated by the cycle counting. Although a theoretical fatigue limit has been proposed, with the introduction of new test equipment for high frequency, the prediction has been improved at low load levels. Although this stress couldn’t damage the components by itself, the accumulated damage induced by high loads can be propagated by such small loads. The correction factors for the slope depend on the material [18].
Schematic spectrums versus components S-N curves: (a) constant amplitude, (b) variable amplitude.
Component life testing is commonly designed to validate fatigue strength of a component based on a target customer usage and is based on loads acting on components. The measurement period is usually not long enough to be used directly in a test. The main target for extrapolated signals is based on time measurement restrictions and problems such as synchronicity, spikes, drifts observed on measurement devices. For this reason, the most representative road or proving ground is determined and that is extrapolated to reach the kilometers of the total life.
The advantages of finite element simulation are mainly in the early stages of design where the prototypes are not yet available, and also to improve its design without physical components. But also in this case, the loads for variable or spectrum as well as constant amplitude are developed to correlate with the accelerated tests. All the factors are evaluated in physical tests, and the results are analyzed through statistical results.
The load measures are extrapolated to the requirement. These spectrums are evaluated to include all the behaviors, as is shown in Figure 12. Spectrum test is developed using different loads from the users and different responses are taken into account.
Spectrum development target.
Schematic spectrum development.
The repetitions of the cycles are found using the linear damage rule of Miner (Eq. (7)), and damage is evaluated using the ratio of the loads
The damage could be reached when the summation is 1 and there is an effect of sequence load. Depending on sequence effect loads, the damage can be reached too with values above or below 1 [4]. The failure is observed when there is a physical crack.
Figure 2 shows that the first point to acquire information is to install measurement devices on the component or in its vicinity, to obtain the responses of the components that induce stress. To obtain the most important information we install the measurement devices at the main stress points. To do this, it is necessary to perform a finite element simulation in order to get the point and the direction of the stresses. Figure 14 shows the typical process used to perform this kind of simulation.
General procedure for simulation.
The components evaluated can be from different materials and built with different manufacturing process. In the next figure are shown instrumented components with a point selection from finite element evaluation [7]. Then to find the point and direction of the main stresses, components are instrumented with strain gauges. Its nominal resistance is 120 or 350 ohms. Higher resistance can be used for base material with low heat conductivity and higher voltage excitation than 10 Volts can be mainly used in environments with high electrical noise [18]. Figure 15 shows chassis components instrumented, Figure 15a the rear subframe for a rigid axle, Figure 15b a front axle steering knuckle and Figure 15c a frontal axle track control arm.
Instrumented components with strain gauges, (a) rear rigid axle subframe, (b) steering knuckle, and (c) track control arm.
The accelerated tests are developed to reduce the time and complexity of the test in order to have faster results. Figure 16 shows a test stand to evaluate a frontal axle track control arm.
Durability test stand for frontal track control arm.
The information collected from the strain gauges can be used to evaluate the component, perform a correlation with virtual or analytical tools and build a spectrum. In not all the cases, can we directly measure the microstrain to validate the virtual simulation. The acceleration can be used to validate the finite element model with experimental acceleration results. With this validation, the stresses are found in a virtual way and can be used to perform the real-life prediction [4].
In the next part, the process to develop an accelerated test is shown. The time history in Figure 17 shows the raw data time history to evaluate a track control arm, its main characteristics is a range of 42,354 N, maximum value of 21,473.6 N and minimum value of −20,880.8 N and the time length is 249.9 s.
Raw data time history for uniaxial test of track control arm.
The information acquired is then analyzed to eliminate unnecessary information such a noise. To do this, we apply filters, and perform statistical analyses. For a structural analysis it is necessary to have a low pass filter of 100 Hz [19]. To evaluate the changes after applying the filter, it is necessary to perform the statistical analysis using cycle counting tools and evaluate the pseudodamage using the linear damage rule [4]. The results of this evaluation are shown in Figure 18.
Statistical analysis of the raw data compared with the filtered signal (a) cycle counting, (b) cumulative cycle count, and (c)PSD.
After applying the filter, the time history obtained has the next characteristics: range of 41,715.3 N, maximum value of 21,283.5 N and minimum of −20,433.6 N; the time length keeps its length of 249.9 s. The pseudodamage was reduced from 4.55 to 4.41, it means a reduction of 3.07% of damage, taking as a reference the raw signal.
There are many ways to accelerate the test. One of them is to eliminate the loads amplitude that do not apply a high amount of damage. To do this, in the time history, we eliminate the amplitude below 5000 N. Figure 19 shows the process to show the selected areas and the final time history.
Cutting load damage areas from the raw data time history filtered, (a) 0–249.9 s, (b) zoom between 221.7 and 233.6 s.
The cut signal and the raw data and the filtered raw data were compared using statistical analysis as it is shown in Figure 20.
Statistical analysis after cutting loads below 5000 N, (a) cycle counting, (b) cumulative cycle count, and (c) PSD.
After eliminating the amplitudes below 5kN, the new time history has the next characteristics: range of 41,717 N, maximum value of 21,283.5 N and minimum value of −20,433.6 N and the time length is 208.4 s. The pseudodamage has not been modified, while the time has been compressed from 249.9 to 208.4 s (16.6%). This is our target signal to generate the test spectrum, in order to develop the durability test.
To accelerate the test, we can increase the number of repetitions of loads with amplitude high and medium. Figure 21 shows the statistical analysis increasing the medium loads. The time history obtained has the next characteristics: range of 42,340.9 N, maximum value of 21,906.9 N and minimum value of −20,433.9 N; time length of 135.1 s. The damage was increased from 4.55 to 8.52, which means that it was increased by a factor of 1.87, reducing the time by 45.93% with respect to the raw data.
Statistical analysis increasing medium loads, (a) cycle counting, (b) cumulative cycle count, and (c) PSD.
Figure 22 shows the statistical analysis increasing the amplitude of high loads and the number of repetitions of high and medium loads. The time history obtained has the next characteristics: range of 59,124.4 N, maximum amplitude of 30,670.6 N and minimum of −28,543.8 N; time length of 167.7 s. The damage was increased from 4.55 to 39.6. This means that it was increased by a factor of 8.7, reducing the time by 32.89% with respect to the raw data.
Statistical analysis increasing high loads: (a) cycle counting, (b) cumulative cycle count, and (c) PSD.
Figure 23a summarizes the spectrums of the all strategies extrapolated to the time histories, the raw filtered data could be cut at below load levels to reduce the time; the medium and high loads in the signal can be increased, reducing the original time and increasing the damage. Figure 23b shows the schematic techniques to accelerate the test.
Spectrum of the time histories, (a) summary of time test reduction and (b) schematic accelerated test.
An alternative option to represent the spectrum instead of time history is with Matrix Rainflow. Figure 24 shows the four analyzed signals: original (Figure 24a), filtered (Figure 24b), increasing the medium loads (Figure 24c), and increasing the number of reversals in high loads inclusive of above the maximum loads of the raw data (Figure 24d). The major differences are shown in Figure 24c and d for medium and high loads, respectively, and the ranges for medium-to-high loads and its number of repetitions have been increased.
Rainflow matrix (a) raw data, (b) cut filtered signal, (c) medium loads increased, and (d) high loads increased.
For variable amplitude loads, this statistical analysis is used to monitor and guarantee that the loads have been applied correctly. Because it is necessary to build a drive used for the actuators to test the applied loads, the feedback through loads are measured and compared with the desired spectrum, and the drive to control the test actuators is developed through an iteration process [20]. Another way to perform an accelerated test based on the spectrum is using two load levels with a constant amplitude load for each load level. Then these results are plotted in an S-N component curve, and the specimen results are evaluated to predict the fatigue strength and different load levels [21]. Figure 25 shows the experimental results of uniaxial constant amplitude loads of steering knuckle.
Test results in steering knuckle analysis.
In an S-N curve, the percent replication
This value represents the portion of specimens that may be used in the variability to replicate the tests. The recommended values by Lee et al. [18] are as follows:
17–33 for preliminary and exploratory tests,
33–50 for research and development tests,
50–75 for design allowable data tests and
75–88 for reliability tests.
Steering knuckle results shown in Figure 25 have 7 level of loads, and 90 Specimens using Eq. (4) get a percent replication of 92.2. These high values obtained from these results are evaluated to analyze a proposal to estimate an S-N curve. For a component test, recommended samples used depend on the target, for research and development tests 6–12, and for reliability tests 12–24 samples. The minimum samples for two load levels are three specimens for each load level.
The median is the central value of results at each load level, and the tendency is considered at 50% of reliability and is necessary to evaluate it to know the scatter of the factors described in Figure 1 (Eq. (8)).
To evaluate the scatter of the components based on its fatigue results, the standard deviation is evaluated using Eq. (10). To take into consideration, its results have to be between 0.05 and 0.15; for samples without notches, the range is between 0.1 and 0.2, for uniaxial tests the range is between 0.2 and 0.3, while in complex tests, it can reach values between 0.3 and 0.6 [22].
Results of the slope found in tests are compared with the requirement, and changes on the slope affect the behavior at low or high load levels. Figure 26 shows the evaluation of the results with constant amplitude loads.
Evaluation results (a) slope test ktest = slope requirement kreq, (b) ktest< kreq, (c) ktest> kreq.
Accelerated tests are used to reduce cost and time in the development process. It can also be used to monitor the quality of the components during its manufacturing life. Experimental evaluation is mandatory prior to final release and start of production to analyze the scatter of the manufacturing process and prevent failures in service life. The importance of performing variable amplitude loads tests is because the prediction of fatigue life under the complex spectrum loads is not possible by any damage hypothesis. The spectrum to evaluate the components in the tests is developed with the loads from different customers and markets and use conditions. Experimental results show discrepancies even within the same batch of production, and the statistical value to evaluate the reliability of the lot under test is the standard deviation that shows the influence of the factors described in Figure 1. Although the tests are performed under controlled conditions in a laboratory, in specimens with notches, the batch of production is released if the standard deviation of its fatigue results has a maximum value of 0.2. For samples without notches in uniaxial tests, the maximum scatter allowed is 0.3 and 0.6 for complex test [22]. To evaluate the fatigue strength as well as the scatter, it is necessary to perform durability tests, to prevent failures on the service life.
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El-Esawi"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"5482",title:"Soybean",subtitle:"The Basis of Yield, Biomass and Productivity",isOpenForSubmission:!1,hash:"2b6f5b827869f467dda14e78f1c45570",slug:"soybean-the-basis-of-yield-biomass-and-productivity",bookSignature:"Minobu Kasai",coverURL:"https://cdn.intechopen.com/books/images_new/5482.jpg",editedByType:"Edited by",editors:[{id:"29226",title:"Dr.",name:"Minobu",middleName:null,surname:"Kasai",slug:"minobu-kasai",fullName:"Minobu Kasai"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"5463",title:"Advances in International Rice Research",subtitle:null,isOpenForSubmission:!1,hash:"92ccc84a75f33d3dac5e3cd4b6a00474",slug:"advances-in-international-rice-research",bookSignature:"Jinquan Li",coverURL:"https://cdn.intechopen.com/books/images_new/5463.jpg",editedByType:"Edited by",editors:[{id:"96434",title:"Dr.",name:"Jin Quan",middleName:null,surname:"Li",slug:"jin-quan-li",fullName:"Jin Quan Li"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}}],booksByTopicTotal:3,seriesByTopicCollection:[],seriesByTopicTotal:0,mostCitedChapters:[{id:"53518",doi:"10.5772/66744",title:"Application and Conversion of Soybean Hulls",slug:"application-and-conversion-of-soybean-hulls",totalDownloads:2221,totalCrossrefCites:10,totalDimensionsCites:24,abstract:"Soybean is one of the most cultivated crops in the world, with a global production of approximately 240 million tons, generating about 18–20 million tons of hulls, the major by-product of soy industry. The chemical composition of soybean hulls depends on the efficiency of the dehulling process, and so, the soybean hulls may contain variable amounts of cellulose (29–51%), hemicelluloses (10–25%), lignin (1–4%), pectins (4–8%), proteins (11–15%), and minor extractives. This chapter provides a review on the composition and structure of soybean hulls, especially in regard to the application and conversion of the compositions. Current applications of soybean hulls are utilizations to animal feed, treatment of wastewater, dietary fiber, and herbal medicine. The conversion of soybean hulls is concerned with ethanol production, bio-oil, polysaccharides, microfibrils, peroxidase, and oligopeptides. On the basis of the relevant findings, we recommend the use of soybean hulls as important source on environment, energy, animal breeding, materials, chemicals, medicine, and food.",book:{id:"5482",slug:"soybean-the-basis-of-yield-biomass-and-productivity",title:"Soybean",fullTitle:"Soybean - The Basis of Yield, Biomass and Productivity"},signatures:"Hua-Min Liu and Hao-Yang Li",authors:[{id:"190617",title:"Dr.",name:"Hua-Min",middleName:null,surname:"Liu",slug:"hua-min-liu",fullName:"Hua-Min Liu"}]},{id:"54259",doi:"10.5772/67361",title:"Genetics and Genomics of Bacterial Blight Resistance in Rice",slug:"genetics-and-genomics-of-bacterial-blight-resistance-in-rice",totalDownloads:2473,totalCrossrefCites:13,totalDimensionsCites:23,abstract:"Rice is an important food crop for half the world’s population and has been in cultivation for over 10,000 years. During the last few decades, rice has evolved intricate relationships with associated pathogens and pests, bacterial blight (BB) being one of the most important among them. Utilization of resistant varieties with agricultural management practices is a more effective way to control BB. Of the 42 different resistance (R) genes identified to confer BB resistance, 9 have been isolated and cloned, whereas a few of the avirulence genes and a large number of candidate pathogenicity genes have been isolated from Xanthomonas oryzae pv. oryzae. The complete genome sequences of two different rice subspecies japonica and indica and three different races of BB pathogen are available. Therefore, the interaction between rice-Xoo could be deciphered and pave a way to study the molecular aspects of bacterial pathogenesis and host counter measures like innate immunity and R gene–mediated immunity. Although several of the type III effectors of Xoo have been characterized and the host targets of a few of them identified, a relatively large number of candidate effectors remain to be studied and their functional analysis may provide key for developing broad spectrum and durable resistance to BB.",book:{id:"5463",slug:"advances-in-international-rice-research",title:"Advances in International Rice Research",fullTitle:"Advances in International Rice Research"},signatures:"Yogesh Vikal and Dharminder Bhatia",authors:[{id:"189992",title:"Dr.",name:"Yogesh",middleName:null,surname:"Vikal",slug:"yogesh-vikal",fullName:"Yogesh Vikal"},{id:"195667",title:"Dr.",name:"Dharminder",middleName:null,surname:"Bhatia",slug:"dharminder-bhatia",fullName:"Dharminder Bhatia"}]},{id:"53538",doi:"10.5772/66743",title:"Role of Nitrogen on Growth and Seed Yield of Soybean and a New Fertilization Technique to Promote Nitrogen Fixation and Seed Yield",slug:"role-of-nitrogen-on-growth-and-seed-yield-of-soybean-and-a-new-fertilization-technique-to-promote-ni",totalDownloads:3295,totalCrossrefCites:10,totalDimensionsCites:19,abstract:"Soybean is an important crop for human food and feed for livestock. World soybean production is increasing especially in North and South America. Soybean seeds contain a high percentage of protein about 35–40%, and they require a large amount of nitrogen compared with other crops. Soybean plants make root nodules with rhizobia, and rhizobia can fix atmospheric N2 and give the fixed N to the host soybean plants. Also, soybean can absorb nitrogen usually nitrate from soil or fertilizers. The amount of total assimilated nitrogen in shoot is proportional to the soybean seed yield either from nitrogen fixation or from nitrogen absorption, and the nitrogen availability is very important for soybean cultivation. Maintenance of a high and long-term nitrogen fixation activity is very important for a high production of soybean. However, application of chemical nitrogen fertilizers usually depresses nodule formation and nitrogen fixation. Nitrate in direct contact with a nodulated part of roots causes severe inhibition of nodule growth and nitrogen fixation, although a distant part of nodules from nitrate application gives no or little effect. Deep placement of slow-release nitrogen fertilizers, coated urea, or lime nitrogen promoted the growth and seed yield and quality of soybean without depressing nitrogen fixation.",book:{id:"5482",slug:"soybean-the-basis-of-yield-biomass-and-productivity",title:"Soybean",fullTitle:"Soybean - The Basis of Yield, Biomass and Productivity"},signatures:"Takuji Ohyama, Kaushal Tewari, Shinji Ishikawa, Kazuya Tanaka,\nSatoshi Kamiyama, Yuki Ono, Soshi Hatano, Norikuni Ohtake, Kuni\nSueyoshi, Hideo Hasegawa, Takashi Sato, Sayuri Tanabata,\nYoshifumi Nagumo, Yoichi Fujita and Yoshihiko Takahashi",authors:[{id:"30061",title:"Prof.",name:"Takuji",middleName:null,surname:"Ohyama",slug:"takuji-ohyama",fullName:"Takuji Ohyama"},{id:"41349",title:"Dr.",name:"Norikuni",middleName:null,surname:"Ohtake",slug:"norikuni-ohtake",fullName:"Norikuni Ohtake"},{id:"41350",title:"Dr.",name:"Kuni",middleName:null,surname:"Sueyoshi",slug:"kuni-sueyoshi",fullName:"Kuni Sueyoshi"},{id:"41351",title:"Dr.",name:"Yoshihiko",middleName:null,surname:"Takahashi",slug:"yoshihiko-takahashi",fullName:"Yoshihiko Takahashi"},{id:"169171",title:"Dr.",name:"Sayuri",middleName:null,surname:"Tanabata",slug:"sayuri-tanabata",fullName:"Sayuri Tanabata"},{id:"195270",title:"Dr.",name:"Kaushal",middleName:null,surname:"Tewari",slug:"kaushal-tewari",fullName:"Kaushal Tewari"},{id:"195271",title:"Dr.",name:"Shinji",middleName:null,surname:"Ishikawa",slug:"shinji-ishikawa",fullName:"Shinji Ishikawa"},{id:"195272",title:"MSc.",name:"Kazuya",middleName:null,surname:"Tanaka",slug:"kazuya-tanaka",fullName:"Kazuya Tanaka"},{id:"195274",title:"MSc.",name:"Satoshi",middleName:null,surname:"Kamiyama",slug:"satoshi-kamiyama",fullName:"Satoshi Kamiyama"},{id:"195275",title:"BSc.",name:"Yuki",middleName:null,surname:"Ono",slug:"yuki-ono",fullName:"Yuki Ono"},{id:"195276",title:"M.Sc.",name:"Soshi",middleName:null,surname:"Hatano",slug:"soshi-hatano",fullName:"Soshi Hatano"},{id:"195277",title:"Prof.",name:"Hideo",middleName:null,surname:"Hasegawa",slug:"hideo-hasegawa",fullName:"Hideo Hasegawa"},{id:"195278",title:"Prof.",name:"Takashi",middleName:null,surname:"Sato",slug:"takashi-sato",fullName:"Takashi Sato"},{id:"195279",title:"Dr.",name:"Yoshifumi",middleName:null,surname:"Nagumo",slug:"yoshifumi-nagumo",fullName:"Yoshifumi Nagumo"},{id:"195280",title:"MSc.",name:"Yoichi",middleName:null,surname:"Fujita",slug:"yoichi-fujita",fullName:"Yoichi Fujita"}]},{id:"53774",doi:"10.5772/67098",title:"Salt Stress Tolerance in Rice: Emerging Role of Exogenous Phytoprotectants",slug:"salt-stress-tolerance-in-rice-emerging-role-of-exogenous-phytoprotectants",totalDownloads:3281,totalCrossrefCites:8,totalDimensionsCites:19,abstract:"Excess salinity in soil is one of the major environmental factors that limit plant growth and yield of a wide variety of crops including rice. On the basis of tolerance ability toward salinity, rice is considered as salt-sensitive crop, and growth and yield of rice are greatly affected by salinity. In general, rice can tolerate a small amount of saltwater without compromising the growth and yield. However, it greatly depends on the types and species of rice and their growth stage. Salinity-induced ionic and osmotic stresses reduce rate of photosynthesis and consequently cause oxidative stress, which is also responsible for growth reduction. The negative effects of salt stress that mentioned ultimately reduced yield of most crops including rice, except some halophytes. In recent decades, researchers have developed various approaches toward making salt-tolerant rice varieties. Using phytoprotectants is found to be effective in conferring salt tolerance to rice plants. In this chapter, we reviewed the recent reports on different aspects on salt stress tolerance strategies in light of using phytoprotectants.",book:{id:"5463",slug:"advances-in-international-rice-research",title:"Advances in International Rice Research",fullTitle:"Advances in International Rice Research"},signatures:"Anisur Rahman, Kamrun Nahar, Jubayer Al Mahmud, Mirza\nHasanuzzaman, Md. Shahadat Hossain and Masayuki Fujita",authors:[{id:"47687",title:"Prof.",name:"Masayuki",middleName:null,surname:"Fujita",slug:"masayuki-fujita",fullName:"Masayuki Fujita"},{id:"76477",title:"Prof.",name:"Mirza",middleName:null,surname:"Hasanuzzaman",slug:"mirza-hasanuzzaman",fullName:"Mirza Hasanuzzaman"},{id:"166818",title:"MSc.",name:"Kamrun",middleName:null,surname:"Nahar",slug:"kamrun-nahar",fullName:"Kamrun Nahar"},{id:"176201",title:"MSc.",name:"Jubayer-Al-",middleName:null,surname:"Mahmud",slug:"jubayer-al-mahmud",fullName:"Jubayer-Al- Mahmud"},{id:"189983",title:"Dr.",name:"Anisur",middleName:null,surname:"Rahman",slug:"anisur-rahman",fullName:"Anisur Rahman"},{id:"189984",title:"Mr.",name:"Md. Shahadat",middleName:null,surname:"Hossain",slug:"md.-shahadat-hossain",fullName:"Md. Shahadat Hossain"}]},{id:"53124",doi:"10.5772/66450",title:"The Use of Rice in Brewing",slug:"the-use-of-rice-in-brewing",totalDownloads:4530,totalCrossrefCites:11,totalDimensionsCites:17,abstract:"Rice could be a useful raw material for the production of a gluten-free beer-like beverage. In today’s beer brewing industry, rice is primarily used as an adjunct in combination with barley malt. But, recently, there is some information about rice malt for brewing an all-rice malt beer. The use of rice as an adjunct in brewing is described highlighting the quality attributes of the final beer. The rice grain quality attributes of different samples are reported in order to evaluate their attitude to malting and brewing and also considering their enzymatic activity. Then, the different brewing processes to produce all-rice malt beers will be described and the final gluten-free rice beers is evaluated and compared to a barley malt beer. Finally, the levels of major aroma-active components of an all-rice malt beer and the results of the sensory analysis assessing the beer-like character of the rice beverage are reported. The obtained beer samples show a content of volatile compounds comparable with a barley malt beer. The sensory profile of the rice malt beer is similar to a barley malt beer in aroma, taste and mouthfeel.",book:{id:"5463",slug:"advances-in-international-rice-research",title:"Advances in International Rice Research",fullTitle:"Advances in International Rice Research"},signatures:"Ombretta Marconi, Valeria Sileoni, Dayana Ceccaroni and Giuseppe\nPerretti",authors:[{id:"189703",title:"Ph.D.",name:"Ombretta",middleName:null,surname:"Marconi",slug:"ombretta-marconi",fullName:"Ombretta Marconi"},{id:"189706",title:"Dr.",name:"Valeria",middleName:null,surname:"Sileoni",slug:"valeria-sileoni",fullName:"Valeria Sileoni"},{id:"189707",title:"Prof.",name:"Giuseppe",middleName:null,surname:"Perretti",slug:"giuseppe-perretti",fullName:"Giuseppe Perretti"},{id:"190973",title:"Dr.",name:"Dayana",middleName:null,surname:"Ceccaroni",slug:"dayana-ceccaroni",fullName:"Dayana Ceccaroni"}]}],mostDownloadedChaptersLast30Days:[{id:"66478",title:"Mungbean (Vigna radiata L. Wilczek): Retrospect and Prospects",slug:"mungbean-em-vigna-radiata-em-l-wilczek-retrospect-and-prospects",totalDownloads:1195,totalCrossrefCites:1,totalDimensionsCites:3,abstract:"Mungbean (Vigna radiata L. Wilczek) is economically most important crop of Vigna group. It is also known as green gram, golden gram, moong, Chickasaw, Oregon pea, and chop suey bean and this legumes have a strategic position in Southeast Asian countries for nutritional security and sustainable crop production. Being rich in quality protein, minerals and vitamins, they are inseparable ingredients in the diets of a vast majority of Indian population. When supplemented with cereals, they provide a perfect mix of essential amino acids with high biological value. These crops have the ability to fix atmospheric nitrogen (58–109 kg per ha in kg per ha mungbean) in symbiotic association with Rhizobium bacteria, which enables them to meet their own nitrogen requirement and also benefit the succeeding crops. This crop has also been reported to smother weed flora appreciably (20–45%) when intercropped with tall cereals or pigeonpea and consequently, minimize the cost incurred on weed control. On account of short duration and photo-thermo insensitivity, they are considered excellent crops for crop intensification and diversification. A seed of mungbean is highly nutritious containing 24–28% protein, 1.0–1.5% fat, 3.5–4.5% fibre, 4.5–5.5% ash and 59–65% carbohydrates on dry weight basis and provide 334–344 kcal energy. Mungbean protein is considered to be easily digestible. Mungbean are tropical grain legumes widely grown in the sub-tropical countries of South and Southeast Asia. Nevertheless, these crops are cultivated over a wide range of latitudes in the regions where average diurnal temperatures during the growing season are warmer than about 20°C.",book:{id:"7000",slug:"legume-crops-characterization-and-breeding-for-improved-food-security",title:"Legume Crops",fullTitle:"Legume Crops - Characterization and Breeding for Improved Food Security"},signatures:"Suhel Mehandi, Syed Mohd. Quatadah, Sudhakar Prasad Mishra, Indra Prakash Singh, Nagmi Praveen and Namrata Dwivedi",authors:[{id:"275243",title:"Dr.",name:"Suhel",middleName:null,surname:"Mehandi",slug:"suhel-mehandi",fullName:"Suhel Mehandi"},{id:"275245",title:"Dr.",name:"Indra Prakash",middleName:null,surname:"Singh",slug:"indra-prakash-singh",fullName:"Indra Prakash Singh"},{id:"275246",title:"Prof.",name:"Sudhakar",middleName:null,surname:"Prasad Mishra",slug:"sudhakar-prasad-mishra",fullName:"Sudhakar Prasad Mishra"},{id:"290295",title:"Dr.",name:"Syed",middleName:null,surname:"Mohd. Quatadah",slug:"syed-mohd.-quatadah",fullName:"Syed Mohd. Quatadah"},{id:"290728",title:"MSc.",name:"Nagmi",middleName:null,surname:"Praveen",slug:"nagmi-praveen",fullName:"Nagmi Praveen"},{id:"290731",title:"Dr.",name:"Namrata",middleName:null,surname:"Dwivedi",slug:"namrata-dwivedi",fullName:"Namrata Dwivedi"}]},{id:"53518",title:"Application and Conversion of Soybean Hulls",slug:"application-and-conversion-of-soybean-hulls",totalDownloads:2227,totalCrossrefCites:10,totalDimensionsCites:24,abstract:"Soybean is one of the most cultivated crops in the world, with a global production of approximately 240 million tons, generating about 18–20 million tons of hulls, the major by-product of soy industry. The chemical composition of soybean hulls depends on the efficiency of the dehulling process, and so, the soybean hulls may contain variable amounts of cellulose (29–51%), hemicelluloses (10–25%), lignin (1–4%), pectins (4–8%), proteins (11–15%), and minor extractives. This chapter provides a review on the composition and structure of soybean hulls, especially in regard to the application and conversion of the compositions. Current applications of soybean hulls are utilizations to animal feed, treatment of wastewater, dietary fiber, and herbal medicine. The conversion of soybean hulls is concerned with ethanol production, bio-oil, polysaccharides, microfibrils, peroxidase, and oligopeptides. On the basis of the relevant findings, we recommend the use of soybean hulls as important source on environment, energy, animal breeding, materials, chemicals, medicine, and food.",book:{id:"5482",slug:"soybean-the-basis-of-yield-biomass-and-productivity",title:"Soybean",fullTitle:"Soybean - The Basis of Yield, Biomass and Productivity"},signatures:"Hua-Min Liu and Hao-Yang Li",authors:[{id:"190617",title:"Dr.",name:"Hua-Min",middleName:null,surname:"Liu",slug:"hua-min-liu",fullName:"Hua-Min Liu"}]},{id:"54205",title:"The Application of Genomic Approaches in Studying a Bacterial Blight-Resistant Mutant in Rice",slug:"the-application-of-genomic-approaches-in-studying-a-bacterial-blight-resistant-mutant-in-rice",totalDownloads:4065,totalCrossrefCites:2,totalDimensionsCites:3,abstract:"Rice bacterial blight disease (BBD), caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the serious diseases in most rice production regions. In this report, we screened for resistance mutants from the mutation pool of TNG67 variety derived by sodium azide (SA) mutagenesis with phenotype investigation and assisted with fluorescent detection. SA0423 is a mutant of broad range resistance against Xoo for many years; the resistance was studied following the concept of central dogma. The inheritance of resistance was characterized, and three QTLs were mapped onto the genome of SA0423 using simple sequence repeat (SSR) markers and R/qtl by genomic approach. In transcriptomic approach, only one differential expression QTLs (eQTLs) were identified; two differentially expressed proteins (pQTLs) were identified and genetically characterized by proteomics after Xoo challenged in SA0423 mutant. To improve the bacterial blight resistance, makers are developed from QTLs, eQTLs and pQTLs to pyramid the resistance genes through marker-assisted breeding in our rice breeding programs.",book:{id:"5463",slug:"advances-in-international-rice-research",title:"Advances in International Rice Research",fullTitle:"Advances in International Rice Research"},signatures:"Chang-Sheng Wang and Da-Gin Lin",authors:[{id:"189870",title:"Prof.",name:"Chang-Sheng",middleName:null,surname:"Wang",slug:"chang-sheng-wang",fullName:"Chang-Sheng Wang"},{id:"194983",title:"Dr.",name:"Da-Gin",middleName:null,surname:"Lin",slug:"da-gin-lin",fullName:"Da-Gin Lin"}]},{id:"53124",title:"The Use of Rice in Brewing",slug:"the-use-of-rice-in-brewing",totalDownloads:4533,totalCrossrefCites:11,totalDimensionsCites:17,abstract:"Rice could be a useful raw material for the production of a gluten-free beer-like beverage. In today’s beer brewing industry, rice is primarily used as an adjunct in combination with barley malt. But, recently, there is some information about rice malt for brewing an all-rice malt beer. The use of rice as an adjunct in brewing is described highlighting the quality attributes of the final beer. The rice grain quality attributes of different samples are reported in order to evaluate their attitude to malting and brewing and also considering their enzymatic activity. Then, the different brewing processes to produce all-rice malt beers will be described and the final gluten-free rice beers is evaluated and compared to a barley malt beer. Finally, the levels of major aroma-active components of an all-rice malt beer and the results of the sensory analysis assessing the beer-like character of the rice beverage are reported. The obtained beer samples show a content of volatile compounds comparable with a barley malt beer. The sensory profile of the rice malt beer is similar to a barley malt beer in aroma, taste and mouthfeel.",book:{id:"5463",slug:"advances-in-international-rice-research",title:"Advances in International Rice Research",fullTitle:"Advances in International Rice Research"},signatures:"Ombretta Marconi, Valeria Sileoni, Dayana Ceccaroni and Giuseppe\nPerretti",authors:[{id:"189703",title:"Ph.D.",name:"Ombretta",middleName:null,surname:"Marconi",slug:"ombretta-marconi",fullName:"Ombretta Marconi"},{id:"189706",title:"Dr.",name:"Valeria",middleName:null,surname:"Sileoni",slug:"valeria-sileoni",fullName:"Valeria Sileoni"},{id:"189707",title:"Prof.",name:"Giuseppe",middleName:null,surname:"Perretti",slug:"giuseppe-perretti",fullName:"Giuseppe Perretti"},{id:"190973",title:"Dr.",name:"Dayana",middleName:null,surname:"Ceccaroni",slug:"dayana-ceccaroni",fullName:"Dayana Ceccaroni"}]},{id:"53218",title:"Evaluation of Palatability of Cooked Rice",slug:"evaluation-of-palatability-of-cooked-rice",totalDownloads:2265,totalCrossrefCites:4,totalDimensionsCites:7,abstract:"Quality evaluations of rice in Japan are performed by sensory testing and physicochemical measurements. The former is a basic method that requires large amounts of samples and several panelists. The latter is an indirect method that estimates the eating quality based on the chemical composition, cooking quality, gelatinization properties, and physical properties of cooked rice. Satake Co Ltd. developed a taste analyzer in the 1980s that is equipped with a palatability estimation formula that was based on the combination of near‐infrared spectroscopy (NIR) and physicochemical measurements related with sensory test. A novel method to evaluate the quality of the cooked rice is necessary to breed high‐quality rice cultivars and to select the suitable rice for each consumer and each purpose. We try to develop the novel method to evaluate the rice quality using various kinds of apparatus, such as Tensipresser, RVA, NIR, and spectrophotometer. Simple, rapid, and accurate method to evaluate the quality of rice grains is very valuable. We evaluated 16 Japanese and Chinese rice cultivars in terms of their physicochemical properties. Based on these quality evaluations, we concluded that Chinese rice cultivars are characterized by a high protein and that the grain texture after cooking has higher hardness and lower stickiness than Japanese ones reflecting the difference in consumers’ preference. The relationship between the palatability of rice and agronomical condition to preserve the bio‐diversity for Crested Ibis was investigated. Furthermore, the quality of rice grown in Sado Island, Japan, was assayed using rice grains grown in mountainous areas and in the field areas as samples.",book:{id:"5463",slug:"advances-in-international-rice-research",title:"Advances in International Rice Research",fullTitle:"Advances in International Rice Research"},signatures:"Ken'ichi Ohtsubo and Sumiko Nakamura",authors:[{id:"190638",title:"Prof.",name:"Ken\\'Ichi",middleName:null,surname:"Ohtsubo",slug:"ken'ichi-ohtsubo",fullName:"Ken\\'Ichi Ohtsubo"}]}],onlineFirstChaptersFilter:{topicId:"350",limit:6,offset:0},onlineFirstChaptersCollection:[],onlineFirstChaptersTotal:0},preDownload:{success:null,errors:{}},subscriptionForm:{success:null,errors:{}},aboutIntechopen:{},privacyPolicy:{},peerReviewing:{},howOpenAccessPublishingWithIntechopenWorks:{},sponsorshipBooks:{sponsorshipBooks:[],offset:8,limit:8,total:0},allSeries:{pteSeriesList:[{id:"14",title:"Artificial Intelligence",numberOfPublishedBooks:8,numberOfPublishedChapters:87,numberOfOpenTopics:6,numberOfUpcomingTopics:0,issn:"2633-1403",doi:"10.5772/intechopen.79920",isOpenForSubmission:!0},{id:"7",title:"Biomedical Engineering",numberOfPublishedBooks:12,numberOfPublishedChapters:98,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2631-5343",doi:"10.5772/intechopen.71985",isOpenForSubmission:!0}],lsSeriesList:[{id:"11",title:"Biochemistry",numberOfPublishedBooks:27,numberOfPublishedChapters:286,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2632-0983",doi:"10.5772/intechopen.72877",isOpenForSubmission:!0},{id:"25",title:"Environmental Sciences",numberOfPublishedBooks:1,numberOfPublishedChapters:9,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2754-6713",doi:"10.5772/intechopen.100362",isOpenForSubmission:!0},{id:"10",title:"Physiology",numberOfPublishedBooks:11,numberOfPublishedChapters:139,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2631-8261",doi:"10.5772/intechopen.72796",isOpenForSubmission:!0}],hsSeriesList:[{id:"3",title:"Dentistry",numberOfPublishedBooks:8,numberOfPublishedChapters:129,numberOfOpenTopics:0,numberOfUpcomingTopics:2,issn:"2631-6218",doi:"10.5772/intechopen.71199",isOpenForSubmission:!1},{id:"6",title:"Infectious Diseases",numberOfPublishedBooks:13,numberOfPublishedChapters:105,numberOfOpenTopics:3,numberOfUpcomingTopics:1,issn:"2631-6188",doi:"10.5772/intechopen.71852",isOpenForSubmission:!0},{id:"13",title:"Veterinary Medicine and Science",numberOfPublishedBooks:9,numberOfPublishedChapters:101,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2632-0517",doi:"10.5772/intechopen.73681",isOpenForSubmission:!0}],sshSeriesList:[{id:"22",title:"Business, Management and Economics",numberOfPublishedBooks:1,numberOfPublishedChapters:11,numberOfOpenTopics:2,numberOfUpcomingTopics:1,issn:null,doi:"10.5772/intechopen.100359",isOpenForSubmission:!0},{id:"23",title:"Education and Human Development",numberOfPublishedBooks:0,numberOfPublishedChapters:0,numberOfOpenTopics:2,numberOfUpcomingTopics:0,issn:null,doi:"10.5772/intechopen.100360",isOpenForSubmission:!1},{id:"24",title:"Sustainable Development",numberOfPublishedBooks:0,numberOfPublishedChapters:9,numberOfOpenTopics:4,numberOfUpcomingTopics:1,issn:null,doi:"10.5772/intechopen.100361",isOpenForSubmission:!0}],testimonialsList:[{id:"6",text:"It is great to work with the IntechOpen to produce a worthwhile collection of research that also becomes a great educational resource and guide for future research endeavors.",author:{id:"259298",name:"Edward",surname:"Narayan",institutionString:null,profilePictureURL:"https://mts.intechopen.com/storage/users/259298/images/system/259298.jpeg",slug:"edward-narayan",institution:{id:"3",name:"University of Queensland",country:{id:null,name:"Australia"}}}},{id:"13",text:"The collaboration with and support of the technical staff of IntechOpen is fantastic. 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",coverUrl:"https://cdn.intechopen.com/series/covers/22.jpg",latestPublicationDate:"May 11th, 2022",hasOnlineFirst:!1,numberOfPublishedBooks:1,editor:{id:"356540",title:"Prof.",name:"Taufiq",middleName:null,surname:"Choudhry",slug:"taufiq-choudhry",fullName:"Taufiq Choudhry",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y000036X2hvQAC/Profile_Picture_2022-03-14T08:58:03.jpg",biography:"Prof. Choudhry holds a BSc degree in Economics from the University of Iowa, as well as a Masters and Ph.D. in Applied Economics from Clemson University, USA. In January 2006, he became a Professor of Finance at the University of Southampton Business School. He was previously a Professor of Finance at the University of Bradford Management School. He has over 80 articles published in international finance and economics journals. His research interests and specialties include financial econometrics, financial economics, international economics and finance, housing markets, financial markets, among others.",institutionString:null,institution:{name:"University of Southampton",institutionURL:null,country:{name:"United Kingdom"}}},editorTwo:null,editorThree:null},subseries:{paginationCount:3,paginationItems:[{id:"86",title:"Business and Management",coverUrl:"https://cdn.intechopen.com/series_topics/covers/86.jpg",isOpenForSubmission:!0,annualVolume:11970,editor:{id:"128342",title:"Prof.",name:"Vito",middleName:null,surname:"Bobek",slug:"vito-bobek",fullName:"Vito Bobek",profilePictureURL:"https://mts.intechopen.com/storage/users/128342/images/system/128342.jpg",biography:"Dr. Vito Bobek works as an international management professor at the University of Applied Sciences FH Joanneum, Graz, Austria. He has published more than 400 works in his academic career and visited twenty-two universities worldwide as a visiting professor. Dr. Bobek is a member of the editorial boards of six international journals and a member of the Strategic Council of the Minister of Foreign Affairs of the Republic of Slovenia. He has a long history in academia, consulting, and entrepreneurship. His own consulting firm, Palemid, has managed twenty significant projects, such as Cooperation Program Interreg V-A (Slovenia-Austria) and Capacity Building for the Serbian Chamber of Enforcement Agents. He has also participated in many international projects in Italy, Germany, Great Britain, the United States, Spain, Turkey, France, Romania, Croatia, Montenegro, Malaysia, and China. Dr. Bobek is also a co-founder of the Academy of Regional Management in Slovenia.",institutionString:"Universities of Applied Sciences FH Joanneum, Austria",institution:null},editorTwo:{id:"293992",title:"Dr.",name:"Tatjana",middleName:null,surname:"Horvat",slug:"tatjana-horvat",fullName:"Tatjana Horvat",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002hXb0hQAC/Profile_Picture_1642419002203",biography:"Tatjana Horvat works as a professor for accountant and auditing at the University of Primorska, Slovenia. She is a Certified State Internal Auditor (licensed by Ministry of Finance RS) and Certified Internal Auditor for Business Sector and Certified accountant (licensed by Slovenian Institute of Auditors). At the Ministry of Justice of Slovenia, she is a member of examination boards for court expert candidates and judicial appraisers in the following areas: economy/finance, valuation of companies, banking, and forensic investigation of economic operations/accounting. At the leading business newspaper Finance in Slovenia (Swedish ownership), she is the editor and head of the area for business, finance, tax-related articles, and educational programs.",institutionString:null,institution:{name:"University of Primorska",institutionURL:null,country:{name:"Slovenia"}}},editorThree:null},{id:"87",title:"Economics",coverUrl:"https://cdn.intechopen.com/series_topics/covers/87.jpg",isOpenForSubmission:!0,annualVolume:11971,editor:{id:"327730",title:"Prof.",name:"Jaime",middleName:null,surname:"Ortiz",slug:"jaime-ortiz",fullName:"Jaime Ortiz",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y00002zaOKZQA2/Profile_Picture_1642145584421",biography:"Dr. Jaime Ortiz holds degrees from Chile, the Netherlands, and the United States. He has held tenured faculty, distinguished professorship, and executive leadership appointments in several universities around the world. Dr. Ortiz has previously worked for international organizations and non-government entities in economic and business matters, and he has university-wide globalization engagement in more than thirty-six countries. He has advised, among others, the United Nations Development Program, Inter-American Development Bank, Organization of American States, Pre-investment Organization of Latin America and the Caribbean, Technical Cooperation of the Suisse Government, and the World Bank. Dr. Ortiz is the author, co-author, or editor of books, book chapters, textbooks, research monographs and technical reports, and refereed journal articles. He is listed in Who’s Who in the World, Who’s Who in America, Who’s Who in Finance and Business, Who’s Who in Business Higher Education, Who’s Who in American Education, and Who’s Who Directory of Economists. Dr. Ortiz has been a Fulbright Scholar and an MSI Leadership Fellow with the W.K. Kellogg Foundation. His teaching interests revolve around global economies and markets while his research focuses on topics related to development and growth, global business decisions, and the economics of technical innovation.",institutionString:null,institution:{name:"University of Houston",institutionURL:null,country:{name:"United States of America"}}},editorTwo:null,editorThree:null},{id:"88",title:"Marketing",coverUrl:"https://cdn.intechopen.com/series_topics/covers/88.jpg",isOpenForSubmission:!1,annualVolume:null,editor:null,editorTwo:null,editorThree:null}]},overviewPageOFChapters:{paginationCount:48,paginationItems:[{id:"81799",title:"Cross Talk of Purinergic and Immune Signaling: Implication in Inflammatory and Pathogenic Diseases",doi:"10.5772/intechopen.104978",signatures:"Richa Rai",slug:"cross-talk-of-purinergic-and-immune-signaling-implication-in-inflammatory-and-pathogenic-diseases",totalDownloads:6,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Purinergic System",coverURL:"https://cdn.intechopen.com/books/images_new/10801.jpg",subseries:{id:"17",title:"Metabolism"}}},{id:"81764",title:"Involvement of the Purinergic System in Cell Death in Models of Retinopathies",doi:"10.5772/intechopen.103935",signatures:"Douglas Penaforte Cruz, Marinna Garcia Repossi and Lucianne Fragel Madeira",slug:"involvement-of-the-purinergic-system-in-cell-death-in-models-of-retinopathies",totalDownloads:4,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Purinergic System",coverURL:"https://cdn.intechopen.com/books/images_new/10801.jpg",subseries:{id:"17",title:"Metabolism"}}},{id:"81756",title:"Alteration of Cytokines Level and Oxidative Stress Parameters in COVID-19",doi:"10.5772/intechopen.104950",signatures:"Marija Petrusevska, Emilija Atanasovska, Dragica Zendelovska, Aleksandar Eftimov and Katerina Spasovska",slug:"alteration-of-cytokines-level-and-oxidative-stress-parameters-in-covid-19",totalDownloads:5,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Chemokines Updates",coverURL:"https://cdn.intechopen.com/books/images_new/11672.jpg",subseries:{id:"18",title:"Proteomics"}}},{id:"81681",title:"Immunomodulatory Effects of a M2-Conditioned Medium (PRS® CK STORM): Theory on the Possible Complex Mechanism of Action through Anti-Inflammatory Modulation of the TLR System and the Purinergic System",doi:"10.5772/intechopen.104486",signatures:"Juan Pedro Lapuente",slug:"immunomodulatory-effects-of-a-m2-conditioned-medium-prs-ck-storm-theory-on-the-possible-complex-mech",totalDownloads:5,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Purinergic System",coverURL:"https://cdn.intechopen.com/books/images_new/10801.jpg",subseries:{id:"17",title:"Metabolism"}}}]},overviewPagePublishedBooks:{paginationCount:27,paginationItems:[{type:"book",id:"7006",title:"Biochemistry and Health Benefits of Fatty Acids",subtitle:null,coverURL:"https://cdn.intechopen.com/books/images_new/7006.jpg",slug:"biochemistry-and-health-benefits-of-fatty-acids",publishedDate:"December 19th 2018",editedByType:"Edited by",bookSignature:"Viduranga Waisundara",hash:"c93a00abd68b5eba67e5e719f67fd20b",volumeInSeries:1,fullTitle:"Biochemistry and Health Benefits of Fatty Acids",editors:[{id:"194281",title:"Dr.",name:"Viduranga Y.",middleName:null,surname:"Waisundara",slug:"viduranga-y.-waisundara",fullName:"Viduranga Y. Waisundara",profilePictureURL:"https://mts.intechopen.com/storage/users/194281/images/system/194281.jpg",biography:"Dr. Viduranga Waisundara obtained her Ph.D. in Food Science and Technology from the Department of Chemistry, National University of Singapore, in 2010. She was a lecturer at Temasek Polytechnic, Singapore from July 2009 to March 2013. She relocated to her motherland of Sri Lanka and spearheaded the Functional Food Product Development Project at the National Institute of Fundamental Studies from April 2013 to October 2016. She was a senior lecturer on a temporary basis at the Department of Food Technology, Faculty of Technology, Rajarata University of Sri Lanka. She is currently Deputy Principal of the Australian College of Business and Technology – Kandy Campus, Sri Lanka. She is also the Global Harmonization Initiative (GHI) Ambassador to Sri Lanka.",institutionString:"Australian College of Business & Technology",institution:null}]},{type:"book",id:"6820",title:"Keratin",subtitle:null,coverURL:"https://cdn.intechopen.com/books/images_new/6820.jpg",slug:"keratin",publishedDate:"December 19th 2018",editedByType:"Edited by",bookSignature:"Miroslav Blumenberg",hash:"6def75cd4b6b5324a02b6dc0359896d0",volumeInSeries:2,fullTitle:"Keratin",editors:[{id:"31610",title:"Dr.",name:"Miroslav",middleName:null,surname:"Blumenberg",slug:"miroslav-blumenberg",fullName:"Miroslav Blumenberg",profilePictureURL:"https://mts.intechopen.com/storage/users/31610/images/system/31610.jpg",biography:"Miroslav Blumenberg, Ph.D., was born in Subotica and received his BSc in Belgrade, Yugoslavia. He completed his Ph.D. at MIT in Organic Chemistry; he followed up his Ph.D. with two postdoctoral study periods at Stanford University. Since 1983, he has been a faculty member of the RO Perelman Department of Dermatology, NYU School of Medicine, where he is codirector of a training grant in cutaneous biology. Dr. Blumenberg’s research is focused on the epidermis, expression of keratin genes, transcription profiling, keratinocyte differentiation, inflammatory diseases and cancers, and most recently the effects of the microbiome on the skin. 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