João Paulo De Carvalho

By Anielle Christine Almeida Silva, Jerusa Maria de Oliveira, Kelen Talita Romão da Silva, Francisco Rubens Alves dos Santos, João Paulo Santos de Carvalho, Rose Kethelyn Souza Avelino, Eurípedes Alves da Silva Filho, Marcelo Duzzioni, Edigar Henrique Vaz Dias, Fábio de Oliveira, Juliana Rodrigues Machado, Malu Mateus Santos, Marcos Vinícius da Silva, Carlo José Freire de Oliveira, Virmondes Rodrigues Junior, Lucas Anhezini and Noelio Oliveira Dantas

This book chapter will comment on fluorescent reporter proteins and nanocrystals’ applicability as fluorescent markers. Fluorescent reporter proteins in the Drosophila model system offer a degree of specificity that allows monitoring cellular and biochemical phenomena in vivo, such as autophagy, mitophagy, and changes in the redox state of cells. Titanium dioxide (TiO2) nanocrystals (NCs) have several biological applications and emit in the ultraviolet, with doping of europium ions can be visualized in the red luminescence. Therefore, it is possible to monitor nanocrystals in biological systems using different emission channels. CdSe/CdS magic-sized quantum dots (MSQDs) show high luminescence stability in biological systems and can be bioconjugated with biological molecules. Therefore, this chapter will show exciting results of the group using fluorescent proteins and nanocrystals in biological systems.

Part of the book: Bioluminescence