This book chapter will comment on fluorescent reporter proteins and nanocrystals’ applicability as fluorescent markers. Fluorescent reporter proteins in the Drosophila model system offer a degree of specificity that allows monitoring cellular and biochemical phenomena in vivo, such as autophagy, mitophagy, and changes in the redox state of cells. Titanium dioxide (TiO2) nanocrystals (NCs) have several biological applications and emit in the ultraviolet, with doping of europium ions can be visualized in the red luminescence. Therefore, it is possible to monitor nanocrystals in biological systems using different emission channels. CdSe/CdS magic-sized quantum dots (MSQDs) show high luminescence stability in biological systems and can be bioconjugated with biological molecules. Therefore, this chapter will show exciting results of the group using fluorescent proteins and nanocrystals in biological systems.
Part of the book: Bioluminescence
Mass testing for COVID-19 is essential to defining patient management strategies, choosing the best clinical management, and dimensioning strategies for controlling viral dissemination and immunization strategies. Thus, it is of utmost importance to search for devices that allow a quick and reliable diagnosis of low cost that can be transposed from the bench to the bedside, such as biosensors. These devices can help choose the correct clinical management to minimize factors that lead to infected patients developing more severe diseases. The use of nanomaterials to modify biosensors’ surfaces to increase these devices’ sensitivity and their biofunctionality enables high-quality nanotechnological platforms. In addition to the diagnostic benefits, nanotechnological platforms that facilitate the monitoring of anti-SARS-CoV-2 antibodies may be the key to determining loss of protective immune response after an episode of COVID-19, which leads to a possible chance of reinfection, as well as how they can be used to assess and monitor the success of immunization strategies, which are beginning to be administered on a large scale and that the extent and duration of their protection will need to be determined. Therefore, in this chapter, we will cover nanomaterials’ use and their functionalities in the surface design of sensors, thus generating nanotechnological platforms in the various facets of the diagnosis of COVID-19.
Part of the book: Biotechnology to Combat COVID-19
This chapter comments on the advantages of nanobiosensors using nanocrystals in improving electrochemical sensors’ response and their use as theragnostic tools in biomedical applications. The use of nanomaterials to modify electrochemical sensors’ surfaces to increase these devices’ sensitivity and their bio-functionality enables high-quality nanotechnological platforms. Thus, graphene nanostructures and CdSe/CdS magic-sized quantum dots (MSQDs) were shown to improve biosensor’s sensitivity. In addition, the use of CdSe/CdS MSQDs and cobalt ferrite nanocrystals (NCs) as potential tools for drug delivery systems and biocompatible titanium dioxide NCs in osseointegration processes and their bio-location are also demonstrated. So, this chapter shows some impressive results on which the group has been working regarding the applications of nanocrystals to electrochemical sensors and theranostic applications.
Part of the book: Biosignal Processing