It is well known that SiO2-based nanomaterials were widely used as support material for many chemically active species but also for compounds with biological activity such as antibodies and enzymes, due to their lack of toxicity and high surface area. The hybrid materials resulted from associating enzymes with various morphologies of SiO2 inorganic matrix, especially obtained by sol-gel method, are meant to develop a higher enzymatic activity and increased lifetime but also the recovery and reutilization advantage. The present contribution emphasized the synthesis of SiO2 nanomaterials with different morphologies and their physicochemical characteristics including biocatalytic activity of immobilized enzymes on simple SiO2. The morphology-dependent behavior of SiO2 inorganic carriers obtained by sol-gel method was also emphasized. Accordingly, SEM investigations, nitrogen sorption, electrokinetic potential, and spectroscopic measurements are presented. p-Nitrophenyl acetate (p-NPA) was used for testing the enzymatic activity of as-prepared lipase-SiO2 hybrid materials.
Part of the book: Nanomaterials