\r\n\tTo viable rural development has a vital role for rural communities. In the design of policies to be successful that affect them rural people have to decide and implement. According to this, it is a critical point to involve the poor and disadvantaged, along with related stakeholders, agricultural and rural development. Hence, for the sustainable development by international initiatives and all other institutions were searched and to be present the agricultural and related research results. To help support the effort, various governmental and non-governmental agencies established fundings for sustainable rural development research and fostered the development of human well-being goals in rural areas via national and international initiatives. In this context, most efforts resulted in successful cases. This book will intend to provide the reader with a comprehensive overview of the theory, approaches, strategies, and cases, and key elements and challenges of sustainable development, and Bioeconomy, Green and Circular economy for sustainability, and UN SDGs-Agenda 2030 and EU Green Deal.
\r\n
\r\n\tI believe that this work will be fundamental in the field of SDG, and it will be a guiding, idea-generating key for researchers, practitioners, rural community, and policy decision-makers, and I hope that together we will establish sustainable rural life and development around the world. \r\n\t
",isbn:"978-1-80355-421-1",printIsbn:"978-1-80355-420-4",pdfIsbn:"978-1-80355-422-8",doi:null,price:0,priceEur:0,priceUsd:0,slug:null,numberOfPages:0,isOpenForSubmission:!1,isSalesforceBook:!1,hash:"759ff88d0677241044b6c8037b924618",bookSignature:"Prof. Dr. Orhan Özçatalbaş",publishedDate:null,coverURL:"https://cdn.intechopen.com/books/images_new/11253.jpg",keywords:"Theory, Approaches, Social Economic, Environment, Bioeconomy, Green Economy, Human Well-Being, Peace, Green Deal, Transformative Policies, Agriculture, Farmers",numberOfDownloads:474,numberOfWosCitations:0,numberOfCrossrefCitations:0,numberOfDimensionsCitations:0,numberOfTotalCitations:0,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"September 9th 2021",dateEndSecondStepPublish:"October 7th 2021",dateEndThirdStepPublish:"December 6th 2021",dateEndFourthStepPublish:"February 24th 2022",dateEndFifthStepPublish:"April 25th 2022",remainingDaysToSecondStep:"7 months",secondStepPassed:!0,currentStepOfPublishingProcess:5,editedByType:null,kuFlag:!1,biosketch:"Dr. Ozcatalbas studies rural development and extension, ICT, and energy policy. He has been a visiting scientist for Postdoc, at Leibniz Hannover University, Institute of Horticultural Economics. He is a member of the Turkish Agricultural Economics Association, and Association for International Agricultural and Extension Education, Society of Agricultural Economics, Scientific Committee Member of the Turkish Foundation for Combating Soil Erosion.",coeditorOneBiosketch:null,coeditorTwoBiosketch:null,coeditorThreeBiosketch:null,coeditorFourBiosketch:null,coeditorFiveBiosketch:null,editors:[{id:"170206",title:"Prof.",name:"Dr. Orhan",middleName:null,surname:"Özçatalbaş",slug:"dr.-orhan-ozcatalbas",fullName:"Dr. Orhan Özçatalbaş",profilePictureURL:"https://mts.intechopen.com/storage/users/170206/images/system/170206.png",biography:"Dr. Orhan Özçatalbaş graduated from Çukurova University Agricultural Faculty at Adana, Turkey in 1986 and completed his PhD in Agricultural Economics in the same institution in 1994. He joined to the Akdeniz University at Antalya in 1998 as an assistant professor of agricultural economics and promoted to professorship in 2011. Dr. Özçatalbas concentrated his work in the field of rural extension and development starting with his MSc and PhD studies, and ICT in agriculture, and rural tourism and development. He has been as a visitor scientist for Postdoc, in Leibniz Hannover University, Institute of Horticultural Economics (Institut für Gartenbau ökonomie), 1999-2000. Dr. Özçatalbaş’s research was focused on the information systems and rural development, and rural energy policy. Dr. Özçatalbaş is a member of the Turkish Agricultural Economics Association, and Association for International Agricultural and Extension Education, Society of Agricultural Economics, Scientific Committe Member of the Turkish Foundation for Combating Soil Erosion (TEMA). Dr Özçatalbaş is also an editor of the International Journal of Rural Tourism and Development (IRTAD, http://www.turizmvekalkinma.org/ ). He has around 100 papers in national and international journals, as well as 6 book chapters and 2 books. Within the scope of his professional project experience; such as project writing and management, project consultancy, project coordinator for rural development, agricultural policy, extension and organic marketing in national and international projects (TÜBİTAK, EU, FAO etc.).",institutionString:null,position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"3",totalChapterViews:"0",totalEditedBooks:"0",institution:{name:"Akdeniz University",institutionURL:null,country:{name:"Turkey"}}}],coeditorOne:null,coeditorTwo:null,coeditorThree:null,coeditorFour:null,coeditorFive:null,topics:[{id:"7",title:"Business, Management and Economics",slug:"business-management-and-economics"}],chapters:[{id:"81546",title:"A Framework for Facilitating Holistic Interventions for Building Community Resilience to Climate Change for Sustainable Community Development",slug:"a-framework-for-facilitating-holistic-interventions-for-building-community-resilience-to-climate-cha",totalDownloads:18,totalCrossrefCites:0,authors:[null]},{id:"81496",title:"Blending Climate Action and Rural Development in Africa’s Sahel",slug:"blending-climate-action-and-rural-development-in-africa-s-sahel",totalDownloads:16,totalCrossrefCites:0,authors:[null]},{id:"81770",title:"Role of Microcredit in Sustainable Rural Development",slug:"role-of-microcredit-in-sustainable-rural-development",totalDownloads:1,totalCrossrefCites:0,authors:[null]},{id:"80307",title:"The Linear and Nonlinear Relationship between Infrastructure and FDI in India",slug:"the-linear-and-nonlinear-relationship-between-infrastructure-and-fdi-in-india",totalDownloads:59,totalCrossrefCites:0,authors:[null]},{id:"80868",title:"Non-Timber Forest Products as an Alternative to Reduce Income Uncertainty in Rural Households",slug:"non-timber-forest-products-as-an-alternative-to-reduce-income-uncertainty-in-rural-households",totalDownloads:29,totalCrossrefCites:0,authors:[null]},{id:"80101",title:"Building New Rural Areas in Vietnam",slug:"building-new-rural-areas-in-vietnam",totalDownloads:68,totalCrossrefCites:0,authors:[null]},{id:"79989",title:"No Sustainability Without Planning for It: Scope and Dimensions for Sustainable Rural Planning in Zimbabwe",slug:"no-sustainability-without-planning-for-it-scope-and-dimensions-for-sustainable-rural-planning-in-zim",totalDownloads:75,totalCrossrefCites:0,authors:[null]},{id:"79911",title:"Biogas Generation from Co-Digestion Waste Systems: The Role of Water Hyacinth",slug:"biogas-generation-from-co-digestion-waste-systems-the-role-of-water-hyacinth",totalDownloads:112,totalCrossrefCites:0,authors:[null]},{id:"79897",title:"The Hambach Forest in the German Debate on Climate Protection: Is There a Symbolic Value beyond the Environmental Value?",slug:"the-hambach-forest-in-the-german-debate-on-climate-protection-is-there-a-symbolic-value-beyond-the-e",totalDownloads:98,totalCrossrefCites:0,authors:[null]}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"},personalPublishingAssistant:{id:"418641",firstName:"Iva",lastName:"Ribic",middleName:null,title:"M.Sc.",imageUrl:"https://mts.intechopen.com/storage/users/418641/images/16830_n.png",email:"iva.r@intechopen.com",biography:"As an Author Service Manager my responsibilities include monitoring and facilitating all publishing activities for authors and editors. 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1. Introduction
Let us consider the interface S between two media having different electrophysical properties. On each of its side the magnetic-field and magnetic-inductance vectors as well as the electric-field and electric-displacement vectors are finite and continuous; however, at the surface S they can experience a discontinuity of the first kind. Furthermore, at the interface there arise induced surface charges σ and surface currents i (whose vectors lie in the plane tangential to the surface S) under the action of an external electric field.
The existence of a surface charge at the interface S between the two media having different electrophysical properties is clearly demonstrated by the following example. We will consider the traverse of a direct current through a flat capacitor filled with two dielectric materials having relative permittivities ε1 and ε2 and electrical conductivities λ1 and λ2. A direct-current voltage U is applied to the capacitor plates; the total resistance of the capacitor is R (Fig. 1). It is necessary to calculate the surface electric charge induced by the electric current.
From the electric-charge conservation law follows the constancy of flow in a circuit; therefore, the following equation is fulfilled:
λ1En1=λ2En2=U/(RS)E1
where En1and En2 are the normal components of the electric-field vector.
At the interface between the dielectrics, the normal components of the electric-inductance vector change spasmodically under the action of the electric field by a value equal to the value of the induced surface charge σ:
ε0ε1En1−ε0ε2En2=σE2
Figure 1.
Dielectric media inside a flat capacitor
Solving the system of Eqs. (see Equations 1 and 2), we obtain the expression for σ
σ=(U/RS)ε0[(ε1/λ1)−(ε2/λ2)]E3
It follows from (see Equation 3) that the charge σ is determined by the current and the multiplier accounting for the properties of the medium. If
(ε1/λ1)−(ε2/λ2)=0E4
a surface charge σ is not formed. What is more, recent trends are toward increased use of micromachines and engines made from plastic materials, where the appearance of a surface charge is undesirable. For oiling of elements of such machines, it is best to use an oil with a permittivity εoilsatisfying the relation
ε1<εoil<ε2E5
This oil makes it possible to decrease the electrization of the moving machine parts made from dielectric materials. In addition to the charge σ, a contact potential difference arises always independently of the current.
An electric field interacting with a material is investigated with the use of the Maxwell equation (1857)
jtotal=∇×H,∇⋅D=ρE6
−∂B∂t=∇×E,∇⋅B=0E7
wherejtotal=λE+∂D∂t;B=μμ0H;D=εε0E. In this case, at the interface S the above system of equations is supplemented with the boundary conditions (Monzon, I.; Yonte,T.; Sanchez-Soto, L., 2003; Eremin,Y. & Wriedt,T., 2002)
Dn1−Dn2=σE8
Eτ1−Eτ2=0E9
Bn1−Bn2=0E10
Hτ1−Hτ2=[i→τ⋅n→]E11
The indices (subscripts) n and τ denote the normal and tangential components of the vectors to the surface S, and the indices 1 and 2 denote the adjacent media with different electrophysical properties. The index τ denotes any direction tangential to the discontinuity surface. At the same time, a closing relation is absent for the induced surface charge σ, which generates a need for the introduction of an impedance matrix (Wei Hu & Hong Guo, 2002; Danae, D. et al., 2002; Larruquert, J. I., 2001; Koludzija, B. M., 1999; Ehlers, R. A. & Metaxas, A. C., 2003) that is determined experimentally or, in some cases, theoretically from quantum representations (Barta, O.; Pistora, I.; Vesec, I. et al., 2001; Broe, I. & Keller, O., 2002; Keller, 1995; Keller, O., 1995; Keller, O., 1997).
The problem of determination of surface charge and surface current on metal-electrolyte boundaries becomes even more complicated in investigating and modeling nonstationary electrochemical processes, e.g., pulse electrolysis, when lumped parameters L, C, and R cannot be used in principle.
We will show that σ can be calculated using the Maxwell phenomenological macroscopic electromagnetic equations and the electric-charge conservation law accounting for the special features of the interface between the adjacent media.
Separate consideration will be given to ion conductors. In constructing a physicomathematical model, we take into account that E→ and H→ are not independent functions; therefore, the wave equation for E→ or H→ is more preferable than the system of equations (see Equations 6 and 7).
2. Electron conductors. New closing relations on the boundaries of adjacent media
2.1. Generalized wave equation for E→ and conditions on the boundaries in the presence of strong discontinuities of the electromagnetic field
2.1.1. Physicomathematical model
We will formulate a physicomathematical model of propagation of an electromagnetic field in a heterogeneous medium. Let us multiply the left and right sides of the equation for the total current (see Equation 6) by μμ0 and differentiate it with respect to time. Acting by the operator rot on the left and right sides of the first equation of Eq. (see Equation 7) on condition that μ=const we obtain
∂jtotal∂t=1μμ0∇2E−1μμ0grad(divE)E12
In Cartesian coordinates, Eq. (see Equation 12) will take the form
Let us write conditions (see Equations 8–11) in the Cartesian coordinate system:
Dx1−Dx2=σE17
Ey1−Ey2=0E18
Ez1−Ez2=0E19
Bx1−Bx2=0E20
Hy1−Hy2=izE21
Hz1−Hz2=iyE22
where iτ = iyj + izk is the surface-current density, and the coordinate x is directed along the normal to the interface. The densities iy and iz of the surface currents represent the electric charge carried in unit time by a segment of unit length positioned on the surface drawing the current perpendicularly to its direction.
The order of the system of differential equations (see Equations 13–15) is equal to 18. Therefore, at the interface S, it is necessary to set, by and large, nine boundary conditions. Moreover, the three additional conditions (see Equation 17, 21, and 22) containing (prior to the solution) unknown quantities should be fulfilled at this interface. Consequently, the total number of conjugation conditions at the boundary S should be equal to 12 for a correct solution of the problem.
Differentiating expression (see Equation 17) with respect to time and using relation (see Equation 16), we obtain the following condition for the normal components of the total current at the medium-medium interface:
diviτ+jtotalx1=jtotalx2E23
that allows one to disregard the surface charge σ. Let us introduce the arbitrary function f:[f]|x=ξ=f1|x=ξ+0−f2|x=ξ−0. In this case, expression (see Equation 23) will take the form
[diviτ+jtotalx1]|x=ξ=0E24
It is assumed that, at the medium-medium interface, Ex is a continuous function of y and z. Then, differentiating Eq. (see Equation 23) with respect to y and z, we obtain
[∂∂yjtotalx1]|x=ξ=−∂(diviτ)∂yE25
[∂∂zjtotalx1]|x=ξ=−∂(diviτ)∂zE26
Let us differentiate conditions (see Equations 20–22) for the magnetic induction and the magnetic-field strength with respect to time. On condition that B=μμ0H
Using Eq. (see Equation 7) and expressing (see Equation 27) in terms of projections of the electric-field rotor, we obtain
[rotxE]|x=ξ=0and
[∂Ez∂y−∂Ey∂z]|x=ξ=0E28
(28)[1μμ0rotyE]|x=ξ=∂iz∂tor
[1μμ0(∂Ex∂z−∂Ez∂x)]|x=ξ=∂iz∂tE29
(29)[1μμ0rotzE]|x=ξ=∂iz∂tor
[1μμ0(∂Ey∂x−∂Ex∂y)]|x=ξ=∂iz∂tE30
(30)
Here, Eq. (see Equation 28) is the normal projection of the electric-field rotor, Eq. (see Equation 29) is the tangential projection of the rotor on y, and Eq. (see Equation 30) is the rotor projection on z.
Assuming that Ey and Ez are continuous differentiable functions of the coordinates y and z, from conditions (see Equations 18 and 19) we find
In accordance with the condition that the tangential projections of the electric field on z and y are equal and in accordance with conditions (see Equations 18 and 19), the expressions for the densities of the surface currents iz and iy take the form
iz=λ¯Ez|x=ξ,iy=λ¯Ey|x=ξE32
where
λ¯=12(λ1+λ2)|x=ξE33
is the average value of the electrical conductivity at the interface between the adjacent media in accordance with the Dirichlet theorem for a piecewise-smooth, piecewise-differentiable function.
Consequently, formulas (see Equations 31–33) yield
[diviτ→]|x=ξ=0E34
Relation (see Equation 34) and hence the equality of the normal components of the total current were obtained (in a different manner) by G.A. Grinberg and V.A. Fok (Grinberg, G.A. & Fok, V.A., 1948). In this work, it has been shown that condition (34) leads to the equality of the derivatives of the electric field strength along the normal to the surface
[∂Ex∂x]|x=ξ=0E35
With allowance for the foregoing we have twelve conditions at the interface between the adjacent media that are necessary for solving the complete system of equations (see Equations 13–15):
the functions Ey and Ez are determined from Eqs. (see Equations 18 and 19);
Ex is determined from condition (see Equation 24);
the values of ∂Ex⁄∂y, ∂Ex⁄∂z, and ∂Ex⁄∂x are determined from relations (see Equations 25 and 26) with the use of the condition of continuity of the total-current normal component at the interface (see Equation 24) and the continuity of the derivative of the total current with respect to the coordinate x;
the values of ∂Ey⁄∂y, ∂Ey⁄∂z, and ∂Ez⁄∂z are determined from conditions (see Equations 31 and 32) in consequence of the continuity of the tangential components of the electric field along y and z;
the derivatives ∂Ey⁄∂x and ∂Ez⁄∂x are determined from conditions (see Equations 29 and 30) as a consequence of the equality of the tangential components of the electric-field rotor along y and z.
Note that condition (see Equation 23) was used by us in (Grinchik, N. N. & Dostanko, A. P., 2005) in the numerical simulation of the pulsed electrochemical processes in the one-dimensional case. Condition (see Equation 28) for the normal component of the electric-field rotor represents a linear combination of conditions (see Equations 31 and 32); therefore, rotxE = 0 and there is no need to use it in the subsequent discussion. The specificity of the expression for the general law of electric-charge conservation at the interface is that the components ∂Ey⁄∂y and ∂Ez⁄∂z are determined from conditions (see Equations 31 and 32) that follow from the equality and continuity of the tangential components Ey and Ez at the boundary S.
Thus, at the interface between the adjacent media the following conditions are fulfilled: the equality of the total-current normal components; the equality of the tangential projections of the electric-field rotor; the electric-charge conservation law; the equality of the electric-field tangential components and their derivatives in the tangential direction; the equality of the derivatives of the total-current normal components in the direction tangential to the interface between the adjacent media, determined with account for the surface currents and without explicit introduction of a surface charge. They are true at each cross section of the sample being investigated.
2.1.2. Features of calculation of the propagation of electromagnetic waves in layered media
The electromagnetic effects arising at the interface between different media under the action of plane electromagnetic waves have a profound impact on the equipment because all real devices are bounded by the surfaces and are inhomogeneous in space. At the same time, the study of the propagation of waves in layered conducting media and, according to (Born, 1970), in thin films is reduced to the calculation of the reflection and transmission coefficients; the function E(x) is not determined in the thickness of a film, i.e., the geometrical-optics approximation is used.
Since conditions (see Equations 23-32) are true at each cross section of a layered medium, we will use schemes of through counting without an explicit definition of the interface between the media. In this case, it is proposed to calculate Ex at the interface in the following way.
In accordance with Eq. (see Equation 17), Ex1≠Ex2, i.e., Ex(x) experiences a discontinuity of the first kind. Let us determine the strength of the electric field at the discontinuity point x = ξ on condition that Ex(x) is a piecewise-smooth, piecewise-differentiable function having finite one-sided derivatives E′x+(x) andE′x−(x). At the discontinuity points xi,
E′x+(xi)=limΔxi→+0E(xi+Δxi)−E(xi+0)ΔxiE36
E′x−(xi)=limΔxi→−0E(xi+Δxi)−E(xi−0)ΔxiE37
In this case, in accordance with the Dirichlet theorem (Kudryavtsev, 1970), the Fourier series of the function E(x) at each point x, including the discontinuity point ξ, converges and its sum is equal to
Ex=ξ=12[E(ξ−0)+E(ξ+0)]E38
The Dirichlet condition (see Equation 38) also has a physical meaning. In the case of contact of two solid conductors, e.g., dielectrics or electrolytes in different combinations (metal-electrolyte, dielectric-electrolyte, metal-vacuum, and so on), at the interface between the adjacent media there always arises an electric double layer (EDL) with an unknown (as a rule) structure that, however, substantially influences the electrokinetic effects, the rate of the electrochemical processes, and so on. It is significant that, in reality, the electrophysical characteristics λ, ε, and E(x) change uninterruptedly in the electric double layer; therefore, (see Equation 38) is true for the case where the thickness of the electric double layer, i.e., the thickness of the interphase boundary, is much smaller than the characteristic size of a homogeneous medium. In a composite, e.g., in a metal with embedments of dielectric balls, where the concentration of both components is fairly large and their characteristic sizes are small, the interphase boundaries can overlap and condition (see Equation 38) can break down.
If the thickness of the electric double layer is much smaller than the characteristic size L of an object, (see Equation 38) also follows from the condition that E(x) changes linearly in the EDL region. In reality, the thickness of the electric double layer depends on the kind of contacting materials and can comprise several tens of angstroms (Frumkin, 1987). In accordance with the modern views, the outer coat of the electric double layer consists of two parts, the first of which is formed by the ions immediately attracted to the surface of the metal (a "dense" or a "Helmholtz" layer of thickness h), and the second is formed by the ions separated by distances larger than the ion radius from the surface of the layer, and the number of these ions decreases as the distance between them and the interface (the "diffusion layer") increases. The distribution of the potential in the dense and diffusion parts of the electric double layer is exponential in actual practice (Frumkin, 1987), i.e., the condition that E(x) changes linearly breaks down; in this case, the sum of the charges of the dense and diffusion parts of the outer coat of the electric double layer is equal to the charge of its inner coat (the metal surface). However, if the thickness of the electric double layer h is much smaller than the characteristic size of an object, the expansion of E(x) into a power series is valid and one can restrict oneself to the consideration of a linear approximation. In accordance with the more general Dirichlet theorem (1829), a knowledge of this function in the EDL region is not necessary to substantiate Eq. (see Equation 38). Nonetheless, the above-indicated physical features of the electric double layer lend support to the validity of condition (see Equation 37).
The condition at interfaces, analogous to Eq. (see Equation 37), has been obtain earlier (Tikhonov, A. N. & Samarskii, A. A., 1977) for the potential field (where rot E = 0) on the basis of introduction of the surface potential, the use of the Green formula, and the consideration of the discontinuity of the potential of the double layer. In (Tikhonov, A. N. & Samarskii, A. A., 1977), it is also noted that the consideration of the thickness of the double layer and the change in its potential at h/L≪1 makes no sense in general; therefore, it is advantageous to consider, instead of the volume potential, the surface potential of any density. Condition (see Equation 38) can be obtained, as was shown in (Kudryavtsev, 1970), from the more general Dirichlet theorem for a nonpotential vorticity field (Tikhonov, A. N. & Samarskii, A. A., 1977).
Thus, the foregoing and the validity of conditions (see Equations 17-19 and 25-.32) at each cross section of a layered medium show that, for numerical solution of the problem being considered it is advantageous to use schemes of through counting and make the discretization of the medium in such a way that the boundaries of the layers have common points.
The medium was divided into finite elements so that the nodes of a finite-element grid, lying on the separation surface between the media with different electrophysical properties, were shared by these media at a time. In this case, the total currents or the current flows at the interface should be equal if the Dirichlet condition (see Equation 38) is fulfilled.
2.1.3. Results of numerical simulation of the propagation of electromagnetic waves in layered media
Let us analyze the propagation of an electromagnetic wave through a layered medium that consists of several layers with different electrophysical properties in the case where an electromagnetic-radiation source is positioned on the upper plane of the medium. It is assumed that the normal component of the electric-field vector Ex = 0 and its tangential component Ey = a sin (ωt), where a is the electromagnetic-wave amplitude (Fig. 2).
In this example, for the purpose of correct specification of the conditions at the lower boundary of the medium, an additional layer is introduced downstream of layer 6; this layer has a larger conductivity and, therefore, the electromagnetic wave is damped out rapidly in it. In this case, the condition Ey = Ez = 0 can be set at the lower boundary of the medium. The above manipulations were made to limit the size of the medium being considered because, in the general case, the electromagnetic wave is attenuated completely at an infinite distance from the electromagnetic-radiation source.
Numerical calculations of the propagation of an electromagnetic wave in the layered medium with electrophysical parameters ε1 = ε2 = 1, λ1 = 100, λ2 = 1000, and μ1 = μ2 = 1 were carried out. Two values of the cyclic frequency ω = 2π/T were used: in the first case, the electromagnetic-wave frequency was assumed to be equal to ω = 1014 Hz (infrared radiation), and, in the second case, the cyclic frequency was taken to be ω = 109 Hz (radiofrequency radiation).
Figure 2.
Scheme of a layered medium: layers 1, 3, and 5 are characterized by the electrophysical parameters ε1, λ1, and μ1, and layers 2, 4, and 6 — by ε2, λ2, μ2.
As a result of the numerical solution of the system of equations (see Equations 13–15) with the use of conditions S (see Equations 24-34) at the interfaces, we obtained the time dependences of the electric-field strength at different distances from the surface of the layered medium (Fig. 3).
Figure 3.
Time change in the tangential component of the electric-field strength at a distance of 1 μm (1), 5 μm (2), and 10 μm (3) from the surface of the medium at λ 1 = 100, λ 2 = 1000, ε1 = ε 2 = 1, μ1 = μ2 = 1, and ω = 1014 Hz. t, sec.
The results of our simulation (Fig. 4) have shown that a high-frequency electromagnetic wave propagating in a layered medium is damped out rapidly, whereas a low-frequency electromagnetic wave penetrates into such a medium to a greater depth. The model developed was also used for calculating the propagation of a modulated signal of frequency 20 kHz in a layered medium. As a result of our simulation (Fig. 5), we obtained changes in the electric-field strength at different depths of the layered medium, which points to the fact that the model proposed can be used to advantage for calculating the propagation of polyharmonic waves in layered media; such a calculation cannot be performed on the basis of the Helmholtz equation.
Figure 4.
Distribution of the amplitude of the electric-field-strength at the cross section of the layered medium: ω = 1014 (1) and 109 Hz (2). y, μm.
Figure 5.
Time change in the electric-field strength at a distance of 1 (1), 5 (2), and 10 μm (3) from the surface of the medium. t, sec.
The physicomathematical model developed can also be used to advantage for simulation of the propagation of electromagnetic waves in media with complex geometric parameters and large discontinuities of the electromagnetic field (Fig. 6).
Figure 6.
Distribution of the amplitude of the electric-field strength in the two-dimensional medium and in depth at ε1 = 15, ε2 = 20, λ1 = 10-6, λ2 = 10, μ1 = μ2 = 1, and ω= 109 Hz (the dark background denotes medium 1, and the light background – medium 2). x, y, mm; E, V/m.
Figure 6a shows the cross-sectional view of a cellular structure representing a set of parallelepipeds with different cross sections in the form of squares. The parametersof the materials in the large parallelepiped are denoted by index 1, and the parameters of the materials in the small parallelepipeds (the squares in the figure) are denoted byindex 2.
An electromagnetic wave propagates in the parallelepipeds (channels) in the transverse direction. It is seen from Fig. 6b that, in the cellular structure there are "silence regions," where the amplitude of the electromagnetic-wave strength is close to zero, as well as inner regions where the signal has a marked value downstream of the "silence" zone formed as a result of the interference.
2.1.4. Results of numerical simulation of the scattering of electromagnetic waves in angular structures
It is radiolocation and radio-communication problems that are among the main challenges in the set of problems solved using radio-engineering devices.
Knowledge of the space-time characteristics of diffraction fields of electromagnetic waves scattered by an object of location into the environment is necessary for solving successfully any radiolocation problem. Irradiated object have a very intricate architecture and geometric shape of the surface consisting of smooth portions and numerous wedge-shaped for formations of different type-angular joints of smooth portions, surface fractures, sharp edges, etc. – with rounded radii much smaller than the probing-signal wavelength. Therefore, solution of radiolocation problems requires that the methods of calculation of the diffraction fields of electromagnetic waves excited and scattered by different surface portions of the objects, in particular, by wedge-shaped formations, be known, since the latter are among the main sources of scattered waves.
For another topical problem, i.e., radio communication effected between objects, the most difficult are the issues of designing of antennas arranged on an object, since their operating efficiency is closely related to the geometric and radiophysical properties of its surface.
The issues of diffraction of an electromagnetic wave in wedge-shaped regions are the focus of numerous of the problems for a perfectly conducting and impedance wedge for monochromatic waves is representation of the diffraction field in an angular region in the form of a Sommerfeld integral (Kryachko, A.F. et al., 2009).
Substitution of Sommerfeld integrals into the system of boundary conditions gives a system of recurrence functional equations for unknown analytical integrands. The system’s coefficients are Fresnel coefficients defining the reflection of plane media or their refraction into the opposite medium. From the system of functional equations, one determines, in a recurrence manner, sequences of integrand poles and residues in these poles.
The edge diffraction field in both media is determined using a pair of Fredholm-type singular integral equations of the second kind which are obtained from the above-indicated systems of functional equations with subsequent computation of Sommerfeld integrals by the saddle-point approximation. The branching points of the integrands condition the presence of creeping waves excited by the edge of the dielectric wedge.
The proposed method is only true of monochromatic waves and of the approximate Leontovich boundary conditions, when the field of the electromagnetic wave slowly varies from point to point on a wavelength scale (Leontovich, 1948).
We note that the existing approximate Leontovich conditions have a number of other constants and should be used with caution (Leontovich, 1948).
In actual fact, the proposed calculation method does not work in the presence of, e.g., two wedges, when the sharp angles are pointed at each other, i.e., an optical knife, or in diffraction of the electromagnetic wave on a system of parallel lobes, when the gap between the lobes is in the region of microns, and the electromagnetic field is strongly “cut” throughout the space with a step much than the wavelength.
A) Optical Knife
Figure 7 shows the field of an electromagnetic wave in its diffraction on the optical knife. The parameters of the wave at entry and at exit are Ex=104sin(1010t), Ey=104cos (1010t).
The electrophysical characteristics are as follows: the wedge is manufactured from aluminum: ε=1; μ=1; σ=3.774 107 S/m; the ambient medium is air.
The dimensions of the computational domain are 0.1×0.05 m. The calculation time 10-9 sec, and the time step is 10-11 sec.
Numerical solution of the system of equations (13)-(15) yields the dependences of the distribution Ex (x,y) и Ey (x,y) on the optical knife. The calculations results are in good agreement with the existing experimental data and experiments specially conducted at the Department of the Physics and Chemistry of Nonequilibrium Media of the A.V. Luikov Heat and Mass Transfer Institute of the National Academy of Sciences by A.I. Bereznyak.
The experiments were carried out with an optical-range laser and were tentative in character but the obtained experimental photographs of diffraction fields and the calculated results turned out to be in good qualitative agreement. The authors express their thanks to A.I. Bereznyak for the conducting of the experiments.
B) Diffraction Grating
The parameters of the wave and the interfacial conditions are the same, as those for the case “optical knife”. The electrophysical characteristics are as follows: 2D lobes, ε=12; and σ=100 S/m; the ambient medium is air; the characteristics of the prism and the square are identical to those of the lobes.
Figure 8 corresponds to a calculation time of 10-10 sec; the time step is 10-12 sec. Figure 9 corresponds to a calculation time of 10-9 sec; the time step is 10-11 sec.
It is seen from the modeling results that the proposed “comb” cab be used as a filter of a high-frequency signal. Furthermore, we carried out numerical calculations of a modulated signal at a frequency of 20 kHz. The results of the modulated-signal calculations are not given. To analyze the difference scheme for stability was analyzed by the initial data. When the time and space steps are large there appear oscillations of the grid solution and of its “derivatives” (“ripple”) which strongly decrease the accuracy of the scheme. Undoubtedly, this issue calls for separate consideration. The proposed algorithm of solution of Maxwell equations allows circuitry-engineering modeling of high-frequency radio-engineering devices and investigation of the propagation of electromagnetic waves in media of intricate geometry in the presence of strong discontinuities of electromagnetic field.
The result of Para 2.1 were published in part (Grinchik, N.N et al., 2009).
Figure 7.
Mesh, amplitude Ex and isolines, amplitude Ey and isolines of the electromagnetic field strength
Figure 8.
Amplitude Ex and isolines, amplitude Ey and isolines of the electromagnetic field strength
Figure 9.
Amplitude Ex and isolines, amplitude Ey and isolines of the electromagnetic field strength
2.1.5. Conclusions
We were the first to construct a consistent physicomathematical model of propagation of electromagnetic waves in layered media without recourse to the matrices of the induced-surface-charge impedances. This model is based on the Maxwell equations, the electric-charge conservation law, the total-current continuity, and the Dirichlet theorem. Our numerical investigations have shown that the physical and mathematical model proposed can be used to advantage for simulation of the propagation of a high-frequency electromagnetic wave in a medium consisting of layers having different electrophysical properties.
2.2. Wave equation for H→and conditions on the boundaries in the presence of strong discontinuities of the electromagnetic field. numerical modeling of electrodynamic processes in the surface layer
2.2.1. Introduction
During the interaction of an external magnetic field and magnetic abrasive particles, the particles are magnetized, and magnetic dipoles with the moment oriented predominantly along the field are formed. "Chains" along the force lines of the field (Shul’man, Z. P. & Kordonskii,V. I., 1982; Khomich, 2006) appear that periodically act on the processable surface with a frequency ω=l/v. A fixed elemental area of the material periodically experiences the effect of the magnetic field of one direction. Actually, the frequency and duration of the pulse will be still higher because of the rotation of the magnetic abrasive particle due to the presence of the moment of forces on contact and of the friction of the particle against the processable part. In what follows, we will not take into account the effect of rotation.
We assume that the particle velocity on the polisher is v. If the particle radius is r, then the angular frequency is ω=2πv/r, and precisely this frequency determines the frequency of the effect of the variable magnetic field component due to the fact that for a ferromagnetic μ>1. The magnetic permeability μ of ferromagnetics, which are usually used in magnetic abrasive polishing, is measured by thousands of units in weak fields. However, in polishing, the constant external magnetic field is strong and amounts to 105-106 A/m, and in this case the value of μ for compounds of iron and nickel and for Heusler alloy decreases substantially.
Because of the presence of a strong external magnetic field H0 the "small" absolute value of μ of an abrasive particle leads to a periodic "increase" and "decrease" in the normal component of the magnetic induction near the processable surface. In the present work we used neodymium magnets (neodymium-iron-boron) with H0>485,000 A/m. The magnetic permeability of a magnetic abrasive particle based on carbonyl iron was assumed in this case to be equal to μ1=100.
Due to the continuity of the normal magnetic induction component Bn1=Bn2, where Bn1=μ1μ0H1; Bn2=μ2μ0H2. For example, in glasses (μ2=1; therefore at the boundary of contact of the glass with the magnetic abrasive particle an additional variable magnetic field of strength H1 > H0 appears.
In (Levin, M. N. et al., 2003; Orlov, A. M. et al., 2001; Makara, V. A. et al., 2001; Rakomsin, 2000), magnetic field-induced effects in silicon are considered: a nonmonotonic change in the crystal lattice parameters in the surface layer of silicon, the gettering of defects on the surface, the change in the sorption properties of the silicon surface, and the change in the mobility of the edge dislocations and in the microhardness of silicon.
In (Golovin, Yu. I. et al.; Makara,V. A. et al., 2008; Orlov, A. M. et al., 2003), the influence of an electromagnetic field on the domain boundaries, plasticity, strengthening, and on the reduction of metals and alloys was established.
In view of the foregoing, it is of interest to find the relationship between the discrete-impulse actions of a magnetic field of one direction on the surface layer of the processable material that contains domains. According to (Shul’man, Z. P. & Kordonskii,V. I., 1982), the size of domains is as follows: 0.05 μm in iron, 1.5 μm in barium ferrite; 8 μm in the MnBi compound, and 0.5-1 μm in the acicular gamma ferric oxide. According to (Akulov, 1961), the size of a domain may reach 10-6 cm3 (obtained by the method of magnetic metallography).
As a rule, an abrasive exhibits a distinct shape anisotropy, whereas the frequency of the effect is determined by the concentration of abrasive particles in a hydrophobic solution and by the velocity of its motion. We assume that on the surface of a processable crystal the magnetic field strength H(t)=H1 sin4(ωt) + H0.
It is required to find the value of the magnetic field strength in the surface layer that has the characteristics λ1, ε1, and μ1 and contains domains with electrophysical properties λ2, ε2, and μ2. The domains may have the form of a triangular prism, a bar, a cylinder, etc.
2.2.2. Physicomathematical model. wave equation for H→
We will formulate a physicomathematical model of propagation of electromagnetic waves in a heterogeneous medium. The media in contact are considered homogeneous. We operate with the operator rot on the left- and right-hand sides of the first equation for the total current (see Equation 6) and multiply by μ0μ; then we differentiate the second equation in Eq. (see Equation 7) with respect to time. Taking into consideration the solenoidality of the magnetic field (see Equation 7) and the rule of repeated application of the operator ∇ to the vector H, we obtain
μ0εε0∂2H∂t2+λμ0∂H∂t=1μ∇2HE39
In the Cartesian coordinates Eq. (see Equation 39) will have the form
One fundamental electromagnetic field equation is the equationdivB→=0. The use of the Dirichlet theorem for approximation of the value of the magnetic field strength on the boundaries between adjacent media analogously to that of the electric field strength does not necessarily guarantees the observance of the condition of solenoidality of the magnetic field; furthermore, the magnetic properties of heterogeneous media were assumed constant in deriving generalized wave equations. The experience of numerical calculations has shown that when it is necessary to model nonstationary magnetic phenomena it is better in many cases to use a generalized wave equation forE→, accordingly expressing H→(t,r→) by E→(t,r→) and, if need be, to perform backward recalculation toH→(t,r→). This approach is difficult to apply to modeling of heterogeneous media with different magnetic properties, when the magnetic permeability µ is dependent on coordinates.
In media with a weak heterogeneity where μ (x, y, z) is a piecewise continuous quantity, the application of the proposed method of through counting is quite justified. Indeed, the system of equations (see Equations 13-15, 40) yields that the function’s discontinuity on the boundaries between adjacent media is determined by the complexes which will be called the generalized permeability ε*≡εμε0μ0 and the generalized conductivityλ*≡λμμ0. Using the Direchlet theorem for ε* andλ*, we obtain their values on the boundaries between adjacent media and the values for the electric field strength at the discontinuity point (see Equation 38); here, we note that the value of the electric field strength is obtained without solving Maxwell equations. In fact, at the discontinuity point, we use linear interpolation of the function to obtain the values ofε*, λ*, andEx=ξ=12[E(ξ−0)+E(ξ+0)]. Consequently, for piecewise continuous quantity μ (x, y, z), the application of the proposed method of through counting is justified. We note that the equality of the derivatives of the electric field strength along the normal to the surface at the discontinuity point according to Eq. (see Equation 35) holds. When the wave equation for H→ is used for media with different magnetic permeabilities the condition of equality of the derivatives fails, i.e.,
∂Hx∂x|x=ξ−0≠∂Hx∂x|x=ξ+0E41
which is a consequence of Eq. (see Equation 10); therefore, the use of through-counting schemes for the wave equation for H→ is difficult.
The generalized wave equation for E→ contains the term graddivE→ which directly allows for the influence of induced surface charges on the propagation of waves. We note that the proposed method of calculation can be used on condition that there are no built-in space charges and extraneous electromotive forces (Grinberg, G.A. & Fok, V.A., 1948).
By virtue of what has been stated above, for modeling of the propagation of electromagnetic waves in glasses having roughness and defects, we used system (see Equations 13-15) with boundary conditions (see Equations 24-34)
2.2.3. Results of numerical simulation
The physicomathematical model developed can also efficiently be used in modeling the propagation of electromagnetic waves in media with complex geometries and strong electromagnetic field discontinuities.
The transverse cut of a cellular structure represents a set of parallelepipeds and triangular prisms of various cross sections, as depicted in Fig. 10a and 11a. An electromagnetic wave propagates across the direction of parallelepipeds and triangular prisms (channels) along the coordinate x.
Figure 10.
Amplitude Hx and isolines of the magnetic field strength
The size of the investigated two-dimensional object is 14×20∙10-6 m, and the sizes of the domains are 2–4 μm. The frequency of the influence of the magnetic field is ω= 2π∙106, and the strength of the field is
Hx=21⋅105sin4(2π⋅106t)A/mE42
(40)
Figure 11.
Amplitude Hy and isolines of the magnetic field strength
The electrophysical properties are as follows: of the large parallelepiped, μ= 1, ε= 8, σ = 10-9 Ω∙m; of domains, μ= 1, ε= 6, σ = 10-8 Ω∙m. They correspond to the electrophysical properties of glasses.
It was assumed that in a layer of thickness 15-20 μm an electromagnetic wave propagates without attenuation; therefore, on all the faces of the large parallelepiped the fulfillment of condition (see Equation 42) was considered valid. On the faces of the parallelepiped that are parallel to the OX axis condition (see Equation 42) corresponded to the "transverse" tangential component of the wave; on the faces parallel to OY condition (see Equation 40) corresponded to the normal component of the field.
The calculations were carried out with a time step of 10-13 sec up to a time instant of 10-10 sec.
Figures 10a and 11a present the amplitude values of the magnetic field strength along Hx and Hy with a comparison scale, whereas Figs. 11b and 11b present the corresponding isolines. An analysis of these figures shows that at the places of discontinuity, on the wedges, force lines of the electromagnetic field concentrate. According to (Akulov, N. S., 1939), precisely wedges are often the sources and sinks of the vacancies that determine, for example, the hardness and plasticity of a solid body.
Also, we modeled the propagation of waves in media, when domains possess magnetic properties. We assumed, in the calculations, that μ=100; the remaining parameters correspond to the previous example of solution (Fig. 12)
Figure 12.
Amplitude Hx and Hy of the magnetic field strength
Of interest is the interaction of the electromagnetic wave with the rough surface shown in Fig. 13 and 14. As in the previous examples, we observe the concentration of electromagnetic energy on angular structures.
Figure 13.
Amplitude Hx and isolines of the magnetic field strength
Figure 14.
Amplitude Hy and isolines of the magnetic field strength
From Fig. 13 and 14, it is seen that electromagnetic heating of tapered structures may occur in addition to mechanical heating in magnetic abrasive machining.
As we have mentioned above, for investigation of the propagation of electromagnetic waves in nonmagnetic materials, it is more expedient to use the generalized equation forE→. For the purpose of illustration we give an example of numerical calculation of an optical knife with the wave equation for H→ (Fig. 15).
From Fig. 15, it is seen that the actual problem of diffraction on the optical knife remains to be solved, i.e., there is no “glow” on the optical-knife section, which is inconsistent with experimental data.
Figure 15.
Amplitude Hx and Hy of the magnetic field strength
As is known (Bazarov, 1991), in thermodynamically equilibrium systems the temperature T and the electrical φ and chemical μc potentials are constant along the entire system:
gradT=0,gradφ=0,gradμc=0E43
If these conditions are not fulfilled (grad T≠0, grad φ≠0, grad μc≠0), irreversible processes of the transfer of mass, energy, electrical charge, etc. appear in the system.
The chemical potential of the j-th component is determined, for example, as a change of the free energy with a change in the number of moles:
μcj=(∂F/∂nj)T,VE44
Where
dF=−SdT−PdV+HdBE45
The last term in Eq. (see Equation 45) takes into account the change in the free energy of a dielectric due to the change in the magnetic induction. The free energy of a unit volume of the dielectric in the magnetic field in this case has the form
F(T,D)=F0+μμ0H22E46
We assume that changes in the temperature and volume of the dielectric are small. Then the mass flux is determined by a quantity proportional to the gradient of the chemical potential or, according to Eq. (see Equation 45), we obtain
qi=−Dμcgrad(HdB)=−DμcgradWE47
where W=μμ0H22 is the density of the magnetic field in the unit volume of the dielectric.
In magnetic abrasive polishing on the sharp protrusions of domains the gradients of magnetic energy are great, which can lead to the origination of vacancy flows.
An analysis of the results shows that the nonstationary component of the full electromagnetic energy is also concentrated in the region of fractures and wedges, i.e., at the sharp angles of domains, which may lead to the improvement of the structure of the sublayer of the treated surface due to the "micromagnetoplastic" effect. Maximum values of the nonstationary part of the total electromagnetic energy Wmax in the sublayer correspond to a maximum value of the function sin (2π∙106\n\t\t\t\t\t\tt) and occur for the time instants t = (n/4)10-6 sec, where n is the integer, with the value of Wmax for a neodymium magnet and a magnetoabrasive particle on the basis of carboxyl iron amounting to a value of the order of (see Equations 5-6)∙106 J/m3. Having multiplied Wmax by the volume of a domain, vacancy, or atom, we may approximately obtain the corresponding energy. The density of the electromagnetic energy in all of the cases is much smaller than the bonding energy of atoms, 10-18-10-19 J. However, a periodic change in the magnetic field in one direction leads to a ponderomotive force that may influence the motion of various defects and dislocations to create a stable and equilibrium structure of atoms and molecules in magnetic abrasive polishing and, in the long run, in obtaining a surface with improved characteristics due to the "micromagnetoplastic" effect. The result of Para 2.2 were published in part (Grinchik, N.N. et al., 2010).
3. Interaction of nonstationary electric and thermal fields with allowance for relaxation processes
We investigate electric and thermal fields created by macroscopic charges and currents in continuous media. Of practical interest is modeling of local heat releases in media on exposure to a high-frequency electromagnetic field. We should take into account the influence of the energy absorption on the propagation of an electromagnetic wave, since the transfer processes are interrelated.
In an oscillatory circuit with continuously distributed parameters, the energy dissipation is linked (Kolesnikov, 2001) to the dielectric loss due to the dependence of the relative permittivity ε(ω) on frequency. In the general case ε is also complex, and the relationship between the electric displacement and electric field vectors has the form D=ε(ω)E, where ε(ω)= ε\'(ω) – iε\'\'(ω); here, ε\' and ε\'\' are determined experimentally. As of now, the problems of dielectric heating of a continuous medium are reduced in many cases to consideration of an equivalent circuit based on lumped parameters, such as capacitance, inductance, loss angle, and relative-dielectric-loss factor (Skanavi, 1949; Perre P.; Turner I. W., 1996), that are established experimentally.
With this approach, there arise substantial difficulties in determining the temperature field of equivalent circuits. Also, we have polarization and the occurrence of an electric double layer of a prescribed electric moment in contact of media with different properties. Equivalent circuits in lamellar media additionally involve empirical lumped parameters: surface capacitance and surface resistance (Jaeger, 1977). The total current can always be separated into a dissipative, or conduction current which is in phase with the applied voltage and a displacement current shifted in time relative to the voltage. The exact physical meaning of these components of the current is largely dependent on selection of an equivalent electric circuit. A unique equivalent circuit – series or parallel connection of the capacitor, the resistor, and the inductor – does not exist; it is determined by a more or less adequate agreement with experimental data.
In the case of electrolytic capacitors, the role of one plate is played by the electric double layer with a specific resistance much higher than the resistance of metallic plates. Therefore, decrease in the capacitance with frequency is observed, for such capacitors, even in the acoustic-frequency range (Jaeger, 1977). Circuits equivalent to an electrolytic capacitor are very bulky: up to 12 R, L, and C elements can be counted in them; therefore, it is difficult to obtain a true value of, e.g., the electrolyte capacitance. In (Jaeger, 1977) experimental methods of measurement of the dielectric properties of electrolyte solutions at different frequencies are given and ε\' and ε" are determined. The frequency dependence of dispersion and absorption are essentially different consequences of one phenomenon: “dielectric-polarization inertia” (Jaeger, 1977). In actual fact, the dependence ε(ω) is attributable to the presence of the resistance of the electric double layer and to the electrochemical cell in the electrolytic capacitor being a system with continuously distributed parameters, in which the signal velocity is a finite quantity.
Actually, ε\' and ε" are certain integral characteristics of a material at a prescribed constant temperature, which are determined by the geometry of the sample and the properties of the electric double layer. It is common knowledge that in the case of a field arbitrarily dependent on time any reliable calculation of the absorbed energy in terms of ε(ω) turns out to be impossible (Landau, L.D. & Lifshits, E.M., 1982). This can only be done for a specific dependence of the field E on time. For a quasimonochromatic field, we have (Landau, L.D. & Lifshits, E.M., 1982)
where the derivatives with respect to frequency are taken at the carrier frequency ω. We note that for an arbitrary function Е(t), it is difficult to represent it in the form
E(t)=a(t)cosφ(t)E51
since we cannot unambiguously indicate the amplitude а(t) and the phase φ (t). The manner in which Е(t) is decomposed into factors а and cosφ is not clear. Even greater difficulties appear in the case of going to the complex representation W(t)=U(t)+iV(t) when the real oscillation Е(t) is supplemented with the imaginary part V(t). The arising problems have been considered in (Vakman, D.E. & Vanshtein, L.A., 1977) in detail. In the indicated work, it has been emphasized that certain methods using a complex representation and claiming higher-than-average accuracy become trivial without an unambiguous determination of the amplitude, phase, and frequency.
Summing up the aforesaid, we can state that calculation of the dielectric loss is mainly empirical in character. Construction of the equivalent circuit and allowance for the influence of the electric double layer and for the dependence of electrophysical properties on the field’s frequency are only true of the conditions under which they have been modeled; therefore, these are fundamental difficulties in modeling the propagation and absorption of electromagnetic energy.
As we believe, the release of heat in media on exposure to nonstationary electric fields can be calculated on the basis of allowance for the interaction of electromagnetic and thermal fields as a system with continuously distributed parameters from the field equation and the energy equation which take account of the distinctive features of the boundary between adjacent media. When the electric field interacting with a material medium is considered we use Maxwell equations (see Equations 6–7). We assume that space charges are absent from the continuous medium at the initial instant of time and they do not appear throughout the process. The energy equation will be represented in the form
ρCpdTdt=div[k(T)grad(T)]+QE52
where Q is the dissipation of electromagnetic energy.
According to (Choo, 1962), the electromagnetic energy converted to heat is determined by the expression
Q=ρ[Eddt(Dρ)+Hddt(Bρ)]+JqEE53
In deriving this formula, we used the nonrelativistic approximation of Minkowski’s theory. If ε, μ, and ρ = const, there is no heat release; therefore, the intrinsic dielectric loss is linked to the introduction of ε\'(ω) and ε"(ω). The quantity Q is affected by the change in the density of the substance ρ(T).
A characteristic feature of high frequencies is the lag of the polarization field behind the charge in the electric field in time. Therefore, the electric-polarization vector is expediently determined by solution of the equation P(t+τe)=(ε-1)ε0Е(t) with allowance for the time of electric relaxation of dipoles τe. Restricting ourselves to the first term of the expansion P(t+τe) in a Taylor series, from this equation, we obtain
P(t)+τedP(t)dt=(ε−1)ε0E(t)E54
The solution (see Equation 54), on condition that Р=0 at the initial instant of time, will take the form
P=(ε−1)ε0τe∫t0tE(τ)e−(t−τ)/τedτE55
It is noteworthy that Eq. (see Equation 54) is based on the classical Debay model. According to this model, particles of a substance possess a constant electric dipole moment. The indicated polarization mechanism involves partial arrangement of dipoles along the electric field, which is opposed by the process of disorientation of dipoles because of thermal collisions. The restoring “force”, in accordance with Eq. (see Equation 54), does not lead to oscillations of electric polarization. It acts as if constant electric dipoles possessed strong damping.
Molecules of many liquids and solids possess the Debay relaxation polarizability. Initially polarization aggregates of Debay oscillators turn back to the equilibrium state P(t)=Р(0)ехр(-t/τe).
A dielectric is characterized, as a rule, by a large set of relaxation times with a characteristic distribution function, since the potential barrier limiting the motion of weakly coupled ions may have different values (Skanavi, 1949); therefore, the mean relaxation time of the ensemble of interacting dipoles should be meant by τe in Eq. (see Equation 54).
To eliminate the influence of initial conditions and transient processes we set t0 = -∞, Е(∞)=0, Н(∞)=0, as it is usually done. If the boundary regime acts for a fairly long time, the influence of initial data becomes weaker with time owing to the friction inherent in every real physical system. Thus, we naturally arrive at the problem without the initial conditions:
P=(ε−1)ε0τe∫−∞tE(τ)e−(t−τ)/τedτE56
Let us consider the case of the harmonic field Е = Е0sinωt; then, using Eq. (see Equation 56) we have, for the electric induction vector
The electric induction vector is essentially the sum of two absolutely different physical quantities: the field strength and the polarization of a unit volume of the medium.
If the change in the density of the substance is small, we obtain, from formula (see Equation 53), for the local instantaneous heat release
when we write the mean value of Q over the total period Т:
Q=12E02(ε−1)ε01+ω2τe2ω2τe+λE2/2E59
For high frequencies (ω→∞), heat release ceases to be dependent on frequency, which is consistent with formula (see Equation 59) and experiment (Skanavi, 1949).
When the relaxation equation for the electric field is used we must also take account of the delay of the magnetic field, when the magnetic polarization lags behind the change in the strength of the external magnetic field:
I(t)+τidI(t)dt=μμ0H(t)E60
Formula (see Equation 59) is well known in the literature; it has been obtained by us without introducing complex parameters. In the case of “strong” heating of a material where the electrophysical properties of the material are dependent on temperature expression (see Equation 54) will have a more complicated form and the expression for Q can only be computed by numerical methods. Furthermore, in the presence of strong field discontinuities, we cannot in principle obtain the expression for Q because of the absence of closing relations for the induced surface charge and the surface current on the boundaries of adjacent media; therefore, the issue of energy relations in macroscopic electrodynamics is difficult, particularly, with allowance for absorption.
Energy relations in a dispersive medium have repeatedly been considered; nonetheless, in the presence of absorption, the issue seems not clearly understood (or at least not sufficiently known), particularly in the determination of the expression of released heat on the boundaries of adjacent media.
Indeed, it is known from the thermodynamics of dielectrics that the differential of the free energy F has the form
dF=−SdT−pdV+EdDE61
If the relative permittivity and the temperature and volume of the dielectric are constant quantities, from Eq. (see Equation 61) we have
F(T,D)=F0+D2/2E62
where F0 is the free energy of the dielectric in the absence of the field.
The change of the internal energy of the dielectric during its polarization at constant temperature and volume can be found from the Gibbs-Helmholtz equation, in which the external parameter D is the electric displacement. Disregarding F0 which is independent of the field strength, we can obtain
U(T,D)=F(T,D)−T(dF/dT)DE63
If the relative dielectric constant is dependent on temperature (ε(T)), we obtain
U(T,D)=ε0E2/2(ε+T(dε/dT)D)E64
Expression (see Equation 64) determines the change in the internal energy of the dielectric in its isothermal polarization but with allowance for the energy transfer to a thermostat, if the polarization causes the dielectric temperature to change. A more detailed substantiation of Eq. (see Equation 64) will be given in the book. In the works on microwave heating, that we know, expression (see Equation 64) is not used.
A characteristic feature of high frequencies is that the polarization field lags behind the change in the external field in time; therefore, the polarization vector is expediently determined by solution of the equation
P(t+τe)=(ε−1+T(dε/dT)D)ε0E(t)E65
With allowance for the relaxation time, i.e., restricting ourselves to the first term of the expansion P(t+τe) in a Taylor series, we obtain
P(t)+τedP(t)/dT=(ε−1+T(dε/dT)D)ε0E(t)E66
In the existing works on microwave heating with the use of complex parameters, they disregard the dependence ε"(T). In (Antonets, I.V.; Kotov, L.N.; Shavrov, V.G. & Shcheglov, V.I., 2009), consideration has been given to the incidence of a one-dimensional wave from a medium with arbitrary complex parameters on one or two boundaries of media whose parameters are also arbitrary. The amplitudes of waves reflected from and transmitted by each boundary have been found. The refection, transmission, and absorption coefficients have been obtained from the wave amplitudes. The well-known proposition that a traditional selection of determinations of the reflection, transmission, and absorption coefficients from energies (reflectivity, transmissivity, and absorptivity) in the case of complex parameters of media comes into conflict with the law of conservation of energy has been confirmed and exemplified. The necessity of allowing for ε"(T) still further complicates the problem of computation of the dissipation of electromagnetic energy in propagation of waves through the boundaries of media with complex parameters.
The proposed method of computation of local heat release is free of the indicated drawbacks and makes it possible, for the first time, to construct a consistent model of propagation of nonmonochromatic waves in a heterogeneous medium with allowance for frequency dispersion without introducing complex parameters.
In closing, we note that a monochromatic wave is infinite in space and time, has infinitesimal energy absorption in a material medium, and transfers infinitesimal energy, which is the idealization of real processes. However with these stringent constraints, too, the problem of propagation of waves through the boundary is open and far from being resolved even when the complex parameters of the medium are introduced and used. In reality, the boundary between adjacent media is not infinitely thin and has finite dimensions of the electric double layers; therefore, approaches based on through-counting schemes for a hyperbolic equation without explicit separation of the boundary between adjacent media are promising.
6. Conclusion
The consistent physicomathematical model of propagation of an electromagnetic wave in a heterogeneous medium has been constructed using the generalized wave equation and the Dirichlet theorem. Twelve conditions at the interfaces of adjacent media were obtained and justified without using a surface charge and surface current in explicit form. The conditions are fulfilled automatically in each section of the heterogeneous medium and are conjugate, which made it possible to use through-counting schemes for calculations. For the first time the effect of concentration of "medium-frequency" waves with a length of the order of hundreds of meters at the fractures and wedges of domains of size 1-3 μm has been established. Numerical calculations of the total electromagnetic energy on the wedges of domains were obtained. It is shown that the energy density in the region of wedges is maximum and in some cases may exert an influence on the motion, sinks, and the source of dislocations and vacancies and, in the final run, improve the near-surface layer of glass due to the "micromagnetoplastic" effect.
The results of these calculations are of special importance for medicine, in particular, when microwaves are used in the therapy of various diseases. For a small, on the average, permissible level of electromagnetic irradiation, the concentration of electromagnetic energy in internal angular structures of a human body (cells, membranes, neurons, interlacements of vessels, etc) is possible.
Acknowledgments
The authors express their gratitude to Corresponding Member of the National Academy of Sciences of Belarus N.V. Pavlyukevich, Corresponding Member of the National Academy of Sciences of Belarus Prof. V.I. Korzyuk and Dr. R. Wojnar for a useful discussion of the work.This work war carried out with financial support from the Belarusian Republic Foundation for Basic Research (grant T10P-122) and from the Science Support Foundation of Poland “Kassa im. Myanowski” (2005).
\n',keywords:null,chapterPDFUrl:"https://cdn.intechopen.com/pdfs/16303.pdf",chapterXML:"https://mts.intechopen.com/source/xml/16303.xml",downloadPdfUrl:"/chapter/pdf-download/16303",previewPdfUrl:"/chapter/pdf-preview/16303",totalDownloads:2024,totalViews:204,totalCrossrefCites:2,totalDimensionsCites:3,totalAltmetricsMentions:0,impactScore:1,impactScorePercentile:62,impactScoreQuartile:3,hasAltmetrics:0,dateSubmitted:"October 16th 2010",dateReviewed:"March 3rd 2011",datePrePublished:null,datePublished:"July 5th 2011",dateFinished:null,readingETA:"0",abstract:null,reviewType:"peer-reviewed",bibtexUrl:"/chapter/bibtex/16303",risUrl:"/chapter/ris/16303",book:{id:"414",slug:"electromagnetic-waves-propagation-in-complex-matter"},signatures:"N.N. Grinchik, O.P. Korogoda, M.S. Khomich, S.V. Ivanova, V.I. Terechov and Yu.N. Grinchik",authors:[{id:"26727",title:"Prof.",name:"Nikolay",middleName:null,surname:"Grinchik",fullName:"Nikolay Grinchik",slug:"nikolay-grinchik",email:"nngrin@yandex.ru",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:{name:"A.V. Luikov Heat and Mass Transfer Institute",institutionURL:null,country:{name:"Belarus"}}},{id:"65740",title:"Mr.",name:"Oleg",middleName:null,surname:"Korogoda",fullName:"Oleg Korogoda",slug:"oleg-korogoda",email:"Karahoda@rambler.ru",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:null},{id:"66136",title:"Prof.",name:"Mikalai",middleName:null,surname:"Khomich",fullName:"Mikalai Khomich",slug:"mikalai-khomich",email:"polimag@mail.ru",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:null},{id:"71024",title:"Dr.",name:"Svetlana",middleName:"Vladimirovna",surname:"Ivanova",fullName:"Svetlana Ivanova",slug:"svetlana-ivanova",email:"sv_ivanova@mail.ru",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:{name:"Moscow Engineering Physics Institute",institutionURL:null,country:{name:"Russia"}}},{id:"81560",title:"Dr",name:"V.I.",middleName:null,surname:"Terechov",fullName:"V.I. Terechov",slug:"v.i.-terechov",email:"terekhov@itp.nsc.ru",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:null},{id:"81561",title:"Dr",name:"Yu.N.",middleName:null,surname:"Grinchik",fullName:"Yu.N. Grinchik",slug:"yu.n.-grinchik",email:"mgrinchik@yandex.ru",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:null},{id:"128374",title:"Prof.",name:"Yu.N.",middleName:null,surname:"Grinchik",fullName:"Yu.N. Grinchik",slug:"yu.n.-grinchik",email:"yun_grinchik@intechweb.com",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:{name:"Belarusian State University",institutionURL:null,country:{name:"Belarus"}}},{id:"128375",title:"Prof.",name:"Oleg",middleName:null,surname:"Korogoda",fullName:"Oleg Korogoda",slug:"oleg-korogoda",email:"oleg_korogoda@intechweb.com",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:null},{id:"128376",title:"Prof.",name:"Nikolay",middleName:null,surname:"Grinchik",fullName:"Nikolay Grinchik",slug:"nikolay-grinchik",email:"nikolay_grinchik@intechweb.com",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:{name:"A.V. Luikov Heat and Mass Transfer Institute",institutionURL:null,country:{name:"Belarus"}}}],sections:[{id:"sec_1",title:"1. Introduction",level:"1"},{id:"sec_2",title:"2. Electron conductors. New closing relations on the boundaries of adjacent media",level:"1"},{id:"sec_2_2",title:"2.1. Generalized wave equation for E→ and conditions on the boundaries in the presence of strong discontinuities of the electromagnetic field",level:"2"},{id:"sec_2_3",title:"2.1.1. Physicomathematical model",level:"3"},{id:"sec_3_3",title:"2.1.2. Features of calculation of the propagation of electromagnetic waves in layered media",level:"3"},{id:"sec_4_3",title:"2.1.3. Results of numerical simulation of the propagation of electromagnetic waves in layered media",level:"3"},{id:"sec_5_3",title:"2.1.4. Results of numerical simulation of the scattering of electromagnetic waves in angular structures",level:"3"},{id:"sec_6_3",title:"2.1.5. Conclusions",level:"3"},{id:"sec_8_2",title:"2.2. Wave equation for H→and conditions on the boundaries in the presence of strong discontinuities of the electromagnetic field. numerical modeling of electrodynamic processes in the surface layer",level:"2"},{id:"sec_8_3",title:"2.2.1. Introduction",level:"3"},{id:"sec_9_3",title:"2.2.2. Physicomathematical model. wave equation for H→",level:"3"},{id:"sec_10_3",title:"2.2.3. Results of numerical simulation",level:"3"},{id:"sec_13",title:"3. Interaction of nonstationary electric and thermal fields with allowance for relaxation processes",level:"1"},{id:"sec_14",title:"6. Conclusion",level:"1"},{id:"sec_15",title:"Acknowledgments",level:"1"}],chapterReferences:[{id:"B1",body:'AkulovN. S.\n\t\t\t\t\t1961\n\t\t\t\t\tDislocations and Plasticity [in Russian]. Minsk: Izd. AN BSSR.'},{id:"B2",body:'AkulovN. S.\n\t\t\t\t\t1939\n\t\t\t\t\tFerromagnetism [in Russian]. Moscow-Leningrad: ONTI.'},{id:"B3",body:'AntonetsI. V.KotovL. N.ShavrovV. G.ShcheglovV. 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A.V. Luikov Heat and Mass Transfer Institute of the National Academy of Science, Minsk, Republic of Belarus
A.V. Luikov Heat and Mass Transfer Institute of the National Academy of Science, Minsk, Republic of Belarus
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1. Introduction
The protein content in cereal grain can vary greatly, from less than 6% to more than 20% in dry matter (DM), and this content depends on several factors such as the type of cereal, variety, agrotechnical conditions and others. These factors can be divided into two major groups, genotype and environment. Today, producers manipulate these factors to obtain grain of good quality and high protein content. For example, it has been found that the rate of maize yield gain is significantly higher after application of 220 kg N ha−1 (0.12 Mg ha−1 year−1) than without fertilization (0.05 Mg ha−1 year−1) [1].
In terms of their functions, there are three types of cereal grain proteins: structural proteins (as membrane proteins), metabolic proteins (as enzymes and enzyme inhibitors) and storage proteins, the largest fraction occurring primarily in starchy endosperm. Storage proteins account for 70–80% of the total protein content in grain and have a unique structure. The primary function of storage proteins is to supply grain embryo with nitrogen and amino acids during germination. However, these proteins are also a valuable food and feed source in human and animal nutrition.
The major storage proteins in cereal grains are called prolamins; the name is derived from their high content of the amino acids proline (Pro) and glutamine (Gln). However, the exact name of prolamins differs in different cereals – in maize prolamins are called zeins, in wheat gliadins, in oat avenins, in barley hordeins, in rye secalins, and in sorghum kafirins.
In human and animal nutrition, cereal crops are an important diet ingredient, and maize is the most commonly used cereal crop. Maize grain is palatable, highly digestible both by humans and animals, and it is an excellent source of metabolizable energy (ME). Although maize grain is low in protein content, the amount used in livestock production makes it an important source of protein in animal diets [2].
2. Maize storage proteins - zeins
Several types of storage proteins are classified in maize depending on their extraction in different solvents. About 70% of maize grain proteins are storage proteins, of which more than 60% are zein (prolamin) proteins. Except zeins, other types of storage proteins include albumins, globulins, and glutelins [3].
First reports describing zeins date back to 1821 when J. Gorham named proteins isolated from maize ‘zeine’. T.B. Osborne, the founder of seed storage protein research, classified zeins as prolamins and developed first extraction methods based on their hydrophobic nature at the beginning of 20 century [4].
Maize zein proteins are located in endosperm within protein aggregates called protein bodies (PBs) consisting of four distinctive zein proteins: alpha (α), beta (β), delta (δ) and gamma (γ) zein (Figure 1). The PBs are part of starch-protein matrix, where starch granules are surrounded with abundant zein proteins in PBs embedded in a matrix of glutelin proteins [7].
Figure 1.
Shematic presentation of zein proteins location within protein bodies. Adapted data from the [5, 6].
As shown in Figure 1, the location of each distinctive zein differs within PB. Alpha zeins are the most abundant group of zein proteins located in the central part of PB together with delta zeins. They account for up to 70% of total zeins. Based on apparent migration rates on SDS-page, two distinctive alpha zeins are defined, namely relative molecular mass (Mr) 19-kDa and Mr 22-kDa alpha zeins [8]. Gamma zeins, the second most abundant zeins (up to 20% in the total zein fraction), are considered to be the most important zeins. This zein class is located on the surface of PB and in small spots within where they stabilize alpha zein core together with beta zein. Gamma zeins are responsible for number and features of PBs and consist of three distinctive proteins: 16-kDa gamma zein, 27-kDa gamma zein and 50-kDa gamma zein [8, 9]. The 27-kDa gamma zein is the most abundant gamma zein, followed by 16-kDa gamma zein and 50-kDa gamma zein, with the latter being in low abundance and for long misidentified as a dimer of the 27-kDa [10]. Based on the apparent migration rates on SDS-page, two distinctive delta zeins (10-kDa and 18-kDa) and one beta zein (15-kDa) are defined [8, 9].
Beta zein and delta zeins are expressed in much lower amounts in maize endosperm. Transcripts encoding alpha zeins account for about 30%, for gamma zeins about 10% whereas for beta and delta zeins 5% and 1.5% of total transcripts, respectively. In the same study, zein specific sequences accounted for almost 50% of the total cDNAs obtained from development of the maize endosperm [10] demonstrating the importance of zein proteins during nitrogen accumulation in maize grain.
2.1 Zein genes
Zein proteins are encoded by a large superfamily of genes. Gamma, beta and delta zeins are encoded by single-copy genes (3 for gamma, 1 for beta, 2 for delta). With some exceptions, two members of delta zeins originate from diploidization, and in quality protein maize (QPM) the genes encoding for 27-kDa gamma zein are duplicated [5]. Alpha zeins are different from the other zein proteins; this group of zein proteins are encoded by four multimember gene families, three for 19-kDa alpha zeins and one for 22-kDa alpha zeins [5]. Studies show large gene clusters that are sometimes disrupted by transposons or other genes [11, 12]. Both 19-kDa and 22-kDa alpha zeins share a common ancestor [12] which underwent amplification and chromosome translocation during evolution [13]. Alpha zeins gene families are located at seven chromosomal sites, but the exact location, and number and organization of genes varies greatly between different inbred lines. Furthermore, not all alpha zein genes are expressed, but only selected members of each alpha zein family [5]. For example, a detailed expression analysis of inbred line of B73 maize showed that only 18 of the 41 alpha zein genes were expressed [14].
2.2 Zein amino acid structure
Zein proteins, and prolamin proteins in general, are rich in amino acids Pro and Gln. However, zeins are devoided of essential amino acids lysine (Lys) and trypthophane (Trp). Thus, a great deal of research has been done to increase the amount of essential amino acids in zein proteins. Besides, the nutritional properties, solubility and chemical structure of zeins are influenced by amino acid characteristics. Zein proteins contain only a few charged amino acids and as a consequence have a hydrophobic nature and thus are insoluble in water. Solubility and chemical structure are important because they define and influence processing and manufacturing of food and feed [15].
Alpha zeins are encoded by large multigene families, and besides Pro and Gln, contain a high proportion of hydrophobic amino acids alanine (Ala) and leucine (Leu). Their structure is largely defined by a series of tandemly repeated peptides of 20 amino acids with nine repeats in the 19-kDa and ten in the 22-kDa alpha zeins. Each repeat is flanked by clusters of Gln residues [6]. Due to their structure, alpha zeins can be extracted with aqueous alcohol [4].
In addition to Pro and Gln, gamma zeins are rich in the cysteine (Cys) which has strong disulfide bonds and thus influencing stability and extractability of zeins [16]. Six highly conserved Cys-rich domains are found in gamma zein proteins [6]. All zein proteins, with the exception of alpha zein proteins, have a high content of sulfur-rich amino acids, which can vary in expression levels among maize cultivars. As mentioned above, gamma zeins are rich in Cys, delta zeins in methionine (Met), and beta zein in both Cys and Met [3].
The amino acid compositions in the most abundant zein proteins are shown in Figure 2.
Figure 2.
Amino acid composition in most abundant zein proteins. Adapted data from the [6].
2.3 The formation of protein bodies
Zein proteins form PBs, insoluble protein aggregates located in the starch-protein matrix. The expression of distinctive zein proteins controls the initiation and development of PBs. Immunogold staining showed that PBs start to aggregate approximately 9 days after pollination as small accretions mainly of gamma and beta zeins. During the PBs growth, alpha zeins and delta zeins enter the PB core and are responsible for the growth and the expansion of the PBs [6]. Each zein protein has a proposed distinctive role in initiation, formation and growth of PBs. The RNA interference (RNAi) technique was used to reduce the expression of a specific zein gene, and it showed that reduction of 22-kDa alpha zein led to PBs with an unusual budding structure [16]. RNAi suppression of both 19-kDa and 22-kDa alpha zein resulted in smaller PBs and with their typical number [17], indicating that 22-kDa can function in PBs morphology and 19-kDa alpha zein can function in PBs growth [5]. RNAi suppression of 27-kDa gamma zein resulted in fewer PBs, while suppression of 16-kDa and 50-kDa gamma zein resulted in smaller PBs but with their normal numbers. Results indicate a significant role of 27-kDa gamma zein in PBs initiation whereas 16-kDa and 50-kDa gamma zeins have a function in PBs expansion [17]. As the inbred line A654, deprived of both delta zeins, has PBs similar to other inbred lines with normal delta zein quantities [16], Li and Song assumed that delta zein has no essential role in the formation of PBs [5]. A study using a yeast two-hybrid system showed strong protein–protein interactions between all gamma zeins and beta zein, weak within alpha zeins, although they both interact strongly with the 10-kDa delta zein, 16-kDa gamma zein and beta zein whereas interacting poorly 27-kDa and 50-kDa gamma zeins [18]. The affinities shown here are consistent with the proposed location and role of each zein protein: 27-kDa gamma zein is responsible for the initiation and is located at the surface of PBs and 16-kDa gamma and beta zein are located towards the inner parts and stabilize alpha/delta zeins of PBs core (Figure 1).
2.4 Analysis of zein proteins
Biologically, zein proteins make a mixture of proteins varying in molecular size, solubility and charge [4]. Solubility of most zeins is good in aqueous-alcohol solutions, 60% isopropanol, 70% ethanol, 95% methanol. However, due to the high disulphide bonds in gamma zeins, this type of zein protein is only extractable when a strong reducing agent, such as 2-mercaptoethanol, is added [19].
When analyzing zein proteins, different extraction procedures are applied: wide range of aqueous ethanol solutions [20, 21, 22] or aqueous isopropanol solutions [23, 24], highly concentrated alkali solutions (pH 11 or above) [25], highly concentrated aqueous urea solutions (8 M) [26], or anionic detergent-containing solutions [20, 26, 27]. The extractions are performed on wide range of extraction temperatures, ranging from 25 to 130°C [20, 21, 26], with or without addition of reducing agents as 2% 2-mercaptoethanol [27] or 10 mM DTT in 25 mM ammonium hydroxide [22]. Solubility-enhancing ingredients, such as 0.0125 M sodium borate [27], 0.5% sodium hydroxide [28], 0.5% sodium bisulfite [24] are also often added.
As zein proteins are a divergent group of proteins, extraction procedures vary significantly. Hence, analysis of zein extracts is complex and include the application of various techniques often used in protein separations. Combination of electrophoresis methods, such as sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), which only provides the molecular weights (MW) [22, 27, 29, 30], or the two-dimensional gel electrophoresis (2DE), which provides insight into MW and charge [22], with or without the mass spectrometry (MS) for protein identification [22, 31], are used in zein analysis. Capillary electrophoresis (CE), the electrophoretic method also used for zein separation, has enabled the identification of more fractions by MS [32]. Other methods of zein separation include chromatographic methods such as reversed-phase HPLC method on C18 column with a gradient of 45 to 75% acetonitrile-ultrapure water, both containing 0.01% trifluoroacetic acid [33] or with acetonitrile-trifuoroacetic acid gradient [34]. Liquid chromatography-mass spectrometry (LC–MS), a method widely used in proteome analysis due to its high sensitivity, was for the first time adopted for zein evaluation in 2020; the authors coupled multi-enzyme digestion with nano-LC–MS/MS [35]. Separation techniques mentioned here are used for the analysis of protein compositions in biological samples of high complexity [22]. Regardless of numerous approaches in zein analysis, the most suitable method will be selected based on desired trait of maize zein proteins.
3. Conditions influencing maize zein content
Factors affecting zein proteins in maize grain can be divided into two main groups – genotype and environment. The genotype is regarded as a primary factor influencing zein properties, while environment (nitrogen fertilization, irrigation, high temperatures, etc.) affect to a lesser extent causing small variability within the same variety.
3.1 Genotype
Maize is usually subdefined according to the kernel characteristic determined by grain vitreousness. Grain vitreousness is an important agronomic trait that influences hardness and post-harvest resistance to insects and fungi, rate of starch digestibility, and semolina yield for food production [36]. It is defined as a ratio of vitreous to floury endosperm and is strongly affected by the type and quantity of zeins within the starch-protein matrix in maize endosperm. The endosperm of flint maize consists mainly of vitreous while floury maize contains almost exclusively floury endosperm [37]. Dent maize hybrids, which are derivatives of flint-floury classes, differ in their ratio of vitreous to floury endosperm.
Zein proteins surround starch granules in the starch-protein matrix in both types of endosperm but with different interactions. In vitreous endosperm, smaller starch granules are tightly packed in multiple and well developed PBs. In contrast, in the floury endosperm, starch granules are larger, and the protein layer is thinner with lesser PBs and with numerous air-filled spaces (Figure 3). The texture differences between vitreous and floury endosperm lead to differences in physical properties of maize samples varying in vitreousness [38]. Kljak and coauthors showed, in a study of 22 maize samples varying in kernel vitreousness (50.23% – 76.41%) and zein content (53.86–86.37 g/kg endosperm DM), that zein proteins, acting through starch-protein interactions, have the most important influence on the maize virtuousness in comparison to the other characteristics of endosperm as amylose content and starch granule size and shape [39].
Figure 3.
Scanning electron micrographs of ground samples of maize hybrids varying in endosperm texture. Larger starch granules with thinner protein layer in the floury endosperm (a) opposite to smaller starch granules with well-developed protein layer in the vitreous endosperm (b). The maize grain endosperm morphological features (starch-protein interactions) were examined visually on 1 mm ground samples using a scanning electron microscope (SEM)(FE-SEM/Mira, Tescan, Brno, Czech Republic) with magnification 5000x.
Zein proteins, as the largest protein fraction in grain, define the quality of maize protein. As mentioned above, zein proteins are devoided of essential amino acids Lys and Trp, and to some extent Met due to the low Met content in alpha zeins. Therefore, despite high yields and sufficient production quantities, amino acid composition limits the usage of maize in nutrition of both humans and monogastric animals. Maize alone cannot provide a balanced meal, and it must be supplemented with sources of essential amino acids, as environmentally questionable soya or other amino acid supplements in the diet of monogastric animals. As a result, maize deficiency in amino acids is increasing costs of food and feed supply worldwide. In order to counteract this, researchers in the last century have focused on regulating the expression and accumulation of zeins in maize.
High Lys and Trp maize mutants have been developed. In the opaque2 (o2) mutant regulating zein O2 transcription factor (TF), alpha zeins have been reduced with compensatory higher amount of non-zein maize proteins. However, those mutants have undesirably soft, opaque and brittle endosperm and their use is limited due to the poor agronomic performance [40]. On the other hand, later developed quality protein maize (QPM) with higher Lys and Trp regained vitreous endosperm and agronomic performances of normal maize [41]. The QPM has zein content different from wild-type or other maize mutants. This maize has a low content of alpha zeins and accumulates a high amount of 27-kDa gamma zeins, which confirms that gamma zeins are essential in PB functionality and preserved hardness in QPM [29]. Liu and coauthors, in their study using genome-wild association study analysis, linkage mapping analysis, and map-based cloning, showed that duplication of gene encoding 27-kDa gamma zein resulted in overexpression of this class of gamma zein protein [30].
The expression of most zein proteins is regulated by one or more TFs, however, maize mutations involving zein proteins may include mutations that can alter the accumulation of zeins in PBs, resulting in abnormal PBs and opaque endosperms or mutations in genes involved in amino acid biosynthesis [5]. For example, affecting myosin XI proteins in opaque1 (O1) leads to an increased number of misshapen PBs [42]. The floury1 (Fl1) mutation leads to a disrupted accumulation of 22-kDa alpha zein in the outer gamma zein region and 19-kDa alpha zein core rather than in the proposed discrete ring at the outer edge of PBs core. However, without changing the PBs size, shape or abundance [43]. Mutant O10 with mutation in opaque10 leads to misshapen PBs, probably caused by disruption of the discrete ring-shaped outer core of PBs containing 22-kDa alpha and 16-kDa gamma zeins [44]. Mutations that lead to altered retention of zeins in PBs include fl2, DeB30, fl4, mucronate1 (mc1) mutation affecting 22-kDa alpha zein, 19-kDa alpha zein, 19-kDa alpha zein and 16-kDa gamma zein, respectively [5].
Mutations affecting amino acid biosynthesis include the Pro1 mutant with inhibited Pro biosynthesis resulting in a lower amount of Pro while the mto140 mutant inhibits the Tyr and Phe biosynthesis. However, both mutations lead to a general reduction of the accumulation of zein proteins and not to a specific zein reduction. The characterization of this type of maize opaque endosperm mutants suggests that amino acid limitations repress zein protein biosynthesis [5]. Other mutations affecting the expression and accumulation of zein proteins on the translation level are opaque 7 (o7) mutant with defective Acyl-CoA synthetase [45] and zmocd1 mutant affecting oxalyl-CoA decarboxylase [46]. Both o7 and zmocd1 have a major impact on amino acid biosynthesis by affecting α-ketoglutaric acid and oxaloacetic acid leading to altered endosperm metabolome and opaque endosperm with reduced zein content [5].
3.2 Environment
Duvick compared the yield of American hybrids grown from 1934 to 2004 and showed that the average annual increase of maize grain yield is 115 kg/ha. The comparison also showed that genotype contributes 50–60% to variability of hybrids (hybrids more resistant to abiotic and biotic stresses) while remaining 40–50% is affected by agricultural technology (fertilizers application, control of diseases and pests, etc.), which demonstrates the importance of not only genetic maize characteristics but also agronomic technology improvements [47].
Agronomic improvements in maize yield have been extensively evaluated; major factors are water management (irrigation) and the application of nitrogen fertilizer depending on environmental conditions. In the United States, for example, the importance of irrigation practices and plant population in the low rainfall Western region of the U.S. corn-belt has been emphasized. In contrast, nitrogen fertilizer use and plant population have been emphasized in the high rainfall Central and Eastern regions [48]. Nitrogen fertilizers are important in modern maize production; the rate of maize yield gain is much higher when 220 kgN ha−1 is applied compared to when no fertilizer is applied (0.12 vs. 0.05 Mg ha−1 year−1, respectively) [1].
Nitrogen fertilizers are required to maintain maize production and soil fertility, however, negative effects of their use on the environment are a global concern, and thus, nitrogen use efficiency (NUE) is an extremely important issue. Nitrogen use efficiency is defined as the amount of grain produced per unit N accumulated above what is provided by soil N mineralization [1]. In modern maize hybrids, the increase in grain yield is often accompanied by a reduction in grain protein concentration [49]. However, Mueller and coauthors in their analyses of NUE in maize hybrids showed that grain yields increased faster than grain N concentration decreased. The same authors concluded that although previous research indicated that maize grain yields and NUE gains over time were primarily due to greater total N accumulation and dilution of grain N accumulation, changes in N accumulation within the plant itself are important to achieve efficient N conversion into grain yield. Key plant factors are increased stem N remobilization and retention of leaf N during reproductive growth [1].
Precipitations and temperature are two key environmental factors affecting maize grain yields and N accumulation. The warm climate will accelerate the phenological development (e.g. leaf appearance), however, drought and high temperatures at pollination as well as during the grain-filling period will reduce yields [50]. In controlled environmental studies when temperature exceeds normal temperatures by only 3°C, maize grain yields were reduced by half [51]. In field studies, rise in 6°C during grain filling period resulted in 13˗88% reduction of maize grain yields and yield loss was much larger under fertilization (authors compared application of 200, 100 and 0 kg N ha−1) [52]. Some studies show that protein concentration is positively correlated with high temperature during cereal grain growth [53]. However, Monjardino and coauthors concluded that heat stress during early stages of endosperm development reduces zein accumulation at synthesis level while later in development had no significant effect on zein quantity. Later during kernel development, the reduction in zeins was mainly result of protein degradation, which appears to be a part of the natural progression of kernel development [54].
4. Zein proteins influence on maize nutritional value
In maize, starch and proteins account for approximately 70% and 10% of grain DM, respectively. Depending the cultivar, starch has the potential for complete digestion in livestock digestive tract [2], and thus, it is the most important energy component in animal diets. Opposite to high energy potential of starch, zein proteins are devoided of essential amino acids Lys and Trp, with the exception for 50-kDa gamma zein which contains 2.7% of Lys. The lack of essential amino acids, Lys, Trp, and to some extent of Met is important characteristic of zein proteins which limits the use of maize protein in food and feed industries. As mentioned earlier, new maize mutants, with different amino acids ratios and protein quality have been developed by maize breeders’ worldwide. The change in amino acid ratios is mostly due to the change of zein proteins compared to other grain proteins [5]. Of maize mutants, QPM, shows excellent characteristics due to the higher Lys and Trp contents and agronomic performances of normal corn. With the use of QPM or high-lysine maize, the need for Lys supplements (soybean meal, synthetic amino acid supplements) is reduced. Although QPM was primarily intended for human nutrition in developing countries, it is nowadays implemented in swine and poultry nutrition as well [2, 41].
In standard diet of monogastric animal, maize grain and soybean meal complement each other. Zein proteins have adequate quantities of sulfur amino acids, Cys and Met (depending on the quantities of alpha zeins) and low Lys and Trp whereas soya has adequate quantities of Lys and Trp and is a relatively poor source of sulfur amino acids [2]. However, it should be noted that higher protein content in maize is often connected with higher zein content, resulting in even lower quantities of Lys and Trp compared to normal maize. Thus, when using maize in monogastric animal nutrition, the amount of essential amino acids relative to energy and total feed intake is more important than the quantity of total protein [55].
The second important aspect of zein influence on maize nutritional value is related to starch rather than to the proteins. As starch granules are embedded in protein layer with PBs, zein poses a physical barrier that can limit the starch availability to digestive enzymes and rumen microorganisms [56]. As a result, starch digestibility in maize varieties with higher zein content will be slower. Maize grain with higher vitreousness has lower digestibility than grains of lower vitreousness containing less zein [7, 56, 57, 58]. Kljak and co-authors in their study on eight yellow high-yield maize hybrids varying in zein content (from 70.3 to 88.7 g kg−1 of total starch) showed that fractional starch digestion rate (kd) in in vitro poultry digestibility experiment correlated negatively to zein content (−0.36, P < 0.05). The authors concluded that when starch granules are embedded in a complex protein matrix, zein limits their accessibility to enzymes and affects the starch digestibility rate to a greater extent than starch properties [59].
Furthermore, when maize grain is subjected to different processing methods as silage production [33, 60] or steam flanking [61], reduction in zein content and destruction of the starch-protein matrix will occur [33]. As a result, starch granules will become easily accessible to digestive enzymes and amylolytic bacteria [61], starch digestibility increases, and starch will be digested in a higher rate [62, 63, 64, 65].
The influence of zein proteins on starch digestibility is becoming a key factor when determining starch efficiency in poultry and swine. Since starch is almost completely digested in their digestive tract, the rate of starch digestibility is the key determinant of animal performance. Weurding showed the importance of evaluation of starch digestion rate for poultry [66]. Higher content of slowly digestible starch appears to support faster, more efficient poultry growth, and the feeds with starch digestibility rates closer to 1.26 h−1 seem to be the most efficient [59]. The results of studies showed that lower digestibility rates are related to a higher amount of zein proteins, and thus, maize hybrids with a higher content of slowly digestible starch are desired. Consequently, when selecting the appropriate maize hybrid for production, in particular for use in animal nutrition, it would not be efficient to know only the quantity of starch but also the quantity of the zein proteins surrounding the starch, since the zein proteins determine the availability of starch and the efficiency of starch digestion.
5. Concluding remarks
Zein proteins, maize storage proteins located in starch-protein matrix of endosperm are paramount for maize nutritional value. They not only define amino acid characteristics of maize grain but are a primary factor affecting starch availability and digestibility. Therefore, genotype and environment effects on zein protein composition and content in grain present a basis to regulate maize nutritional value.
Conflict of interest
The authors declare that there is no conflict of interest.
\n',keywords:"maize grain, zein proteins, starch, amino acid quality, starch digestibility, maize nutritional value, animal nutrition",chapterPDFUrl:"https://cdn.intechopen.com/pdfs/74575.pdf",chapterXML:"https://mts.intechopen.com/source/xml/74575.xml",downloadPdfUrl:"/chapter/pdf-download/74575",previewPdfUrl:"/chapter/pdf-preview/74575",totalDownloads:407,totalViews:0,totalCrossrefCites:0,dateSubmitted:"September 17th 2020",dateReviewed:"December 4th 2020",datePrePublished:"January 15th 2021",datePublished:"September 29th 2021",dateFinished:"December 24th 2020",readingETA:"0",abstract:"Crop grains accumulate significant amounts of nitrogen in the form of storage proteins. Grain storage proteins are not only important in the aspects of germination but also, storage proteins are a valuable food source in human and animal nutrition. This chapter will give insight into genotype and growing conditions influencing the quantity and quality of storage proteins, primarily maize storage proteins the leading cereal by world production. Main storage proteins in cereals are prolamins, and in maize prolamins are called zeins located within the endosperm in protein agglomerations called protein bodies. Four main classes of zein proteins are: alpha, beta, gamma and delta zein. Each of four zein classes has a distinctive position and role within protein bodies. Prolamin proteins define nutritional value of maize grain not only via amino acid quality but also via starch availability. Starch, the most important energy component of maize grain, is located within starch-protein matrix. Within this matrix, starch granules are surrounded by protein bodies that limit starch availability. In this chapter, we will describe how zein proteins influence characteristics of maize grain and nutritional value of maize.",reviewType:"peer-reviewed",bibtexUrl:"/chapter/bibtex/74575",risUrl:"/chapter/ris/74575",signatures:"Marija Duvnjak, Kristina Kljak and Darko Grbeša",book:{id:"10360",type:"book",title:"Nitrogen in Agriculture",subtitle:"Physiological, Agricultural and Ecological Aspects",fullTitle:"Nitrogen in Agriculture - Physiological, Agricultural and Ecological Aspects",slug:"nitrogen-in-agriculture-physiological-agricultural-and-ecological-aspects",publishedDate:"September 29th 2021",bookSignature:"Takuji Ohyama and Kazuyuki Inubushi",coverURL:"https://cdn.intechopen.com/books/images_new/10360.jpg",licenceType:"CC BY 3.0",editedByType:"Edited by",isbn:"978-1-83968-492-0",printIsbn:"978-1-83968-488-3",pdfIsbn:"978-1-83968-493-7",isAvailableForWebshopOrdering:!0,editors:[{id:"30061",title:"Prof.",name:"Takuji",middleName:null,surname:"Ohyama",slug:"takuji-ohyama",fullName:"Takuji Ohyama"}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"}},authors:[{id:"331561",title:"Assistant Prof.",name:"Marija",middleName:null,surname:"Duvnjak",fullName:"Marija Duvnjak",slug:"marija-duvnjak",email:"mduvnjak@agr.hr",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:null},{id:"331834",title:"Prof.",name:"Darko",middleName:null,surname:"Grbeša",fullName:"Darko Grbeša",slug:"darko-grbesa",email:"dgrbesa@agr.hr",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:{name:"University of Zagreb",institutionURL:null,country:{name:"Croatia"}}},{id:"331835",title:"Dr.",name:"Kristina",middleName:null,surname:"Kljak",fullName:"Kristina Kljak",slug:"kristina-kljak",email:"kkljak@agr.hr",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:{name:"University of Zagreb",institutionURL:null,country:{name:"Croatia"}}}],sections:[{id:"sec_1",title:"1. Introduction",level:"1"},{id:"sec_2",title:"2. Maize storage proteins - zeins",level:"1"},{id:"sec_2_2",title:"2.1 Zein genes",level:"2"},{id:"sec_3_2",title:"2.2 Zein amino acid structure",level:"2"},{id:"sec_4_2",title:"2.3 The formation of protein bodies",level:"2"},{id:"sec_5_2",title:"2.4 Analysis of zein proteins",level:"2"},{id:"sec_7",title:"3. Conditions influencing maize zein content",level:"1"},{id:"sec_7_2",title:"3.1 Genotype",level:"2"},{id:"sec_8_2",title:"3.2 Environment",level:"2"},{id:"sec_10",title:"4. Zein proteins influence on maize nutritional value",level:"1"},{id:"sec_11",title:"5. Concluding remarks",level:"1"},{id:"sec_15",title:"Conflict of interest",level:"1"}],chapterReferences:[{id:"B1",body:'Mueller SM, Messina CD, Vyn TJ. Simultaneous gains in grain yield and nitrogen efficiency over 70 years of maize genetic improvement. Scientific Reports. 2018;9(1):1-8. DOI: 10.1038/s41598-019-45485-5'},{id:"B2",body:'Loy DD, Lundy EL. 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Department of Animal Nutrition, University of Zagreb Faculty of Agriculture, Svetošimunska cesta, Zagreb, Croatia
Department of Animal Nutrition, University of Zagreb Faculty of Agriculture, Svetošimunska cesta, Zagreb, Croatia
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In addition to this direct damage, they cause indirect damage to their hosts by transmitting various plant pathogens. There are some 4,500 species of parasitic plants known; out of them, nearly 60% are root parasites and the rest of them parasitise on the shoot parts. Orobanchaceae and Convolvulaceae are the two mostly studied families of parasitic plants; and the parasitic plants are the chief mode for transmission of the phytoplasmas. The parasitic plants have various modes of obtaining nutrition; however, the information about the mechanism(s) involved in the pathogen transmission by the parasitic plants is limited. The latest biotechnolgical advances, such as metagenomics and high througput sequencing, carry immense promise in understanding the host-parasitic plant-pathogen association in deeper details; and initiatives have indeed been taken. Nevertheless, compared to the other pests hindering crop productivity, parasitic plants have not yet been able to gain the needed attention of the plant scientists. 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Open Access publishing helps remove barriers and allows everyone to access valuable information, but article and book processing charges also exclude talented authors and editors who can’t afford to pay. The goal of our Women in Science program is to charge zero APCs, so none of our authors or editors have to pay for publication.
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We are currently in the process of collecting sponsorship. If you have any ideas or would like to help sponsor this ambitious program, we’d love to hear from you. Contact us at info@intechopen.com.
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He is also an editor and editor in chief for various international journals.",institutionString:"Kuwait University",institution:{name:"Kuwait University",country:{name:"Kuwait"}}},{id:"32650",title:"Prof.",name:"Lukas",middleName:"Willem",surname:"Snyman",slug:"lukas-snyman",fullName:"Lukas Snyman",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/32650/images/4136_n.jpg",biography:"Lukas Willem Snyman received his basic education at primary and high schools in South Africa, Eastern Cape. He enrolled at today's Nelson Metropolitan University and graduated from this university with a BSc in Physics and Mathematics, B.Sc Honors in Physics, MSc in Semiconductor Physics, and a Ph.D. in Semiconductor Physics in 1987. After his studies, he chose an academic career and devoted his energy to the teaching of physics to first, second, and third-year students. After positions as a lecturer at the University of Port Elizabeth, he accepted a position as Associate Professor at the University of Pretoria, South Africa.\r\n\r\nIn 1992, he motivates the concept of 'television and computer-based education” as means to reach large student numbers with only the best of teaching expertise and publishes an article on the concept in the SA Journal of Higher Education of 1993 (and later in 2003). The University of Pretoria subsequently approved a series of test projects on the concept with outreach to Mamelodi and Eerste Rust in 1993. In 1994, the University established a 'Unit for Telematic Education ' as a support section for multiple faculties at the University of Pretoria. In subsequent years, the concept of 'telematic education” subsequently becomes well established in academic circles in South Africa, grew in popularity, and is adopted by many universities and colleges throughout South Africa as a medium of enhancing education and training, as a method to reaching out to far out communities, and as a means to enhance study from the home environment.\r\n\r\nProfessor Snyman in subsequent years pursued research in semiconductor physics, semiconductor devices, microelectronics, and optoelectronics.\r\n\r\nIn 2000 he joined the TUT as a full professor. Here served for a period as head of the Department of Electronic Engineering. Here he makes contributions to solar energy development, microwave and optoelectronic device development, silicon photonics, as well as contributions to new mobile telecommunication systems and network planning in SA.\r\n\r\nCurrently, he teaches electronics and telecommunications at the TUT to audiences ranging from first-year students to Ph.D. level.\r\n\r\nFor his research in the field of 'Silicon Photonics” since 1990, he has published (as author and co-author) about thirty internationally reviewed articles in scientific journals, contributed to more than forty international conferences, about 25 South African provisional patents (as inventor and co-inventor), 8 PCT international patent applications until now. Of these, two USA patents applications, two European Patents, two Korean patents, and ten SA patents have been granted. A further 4 USA patents, 5 European patents, 3 Korean patents, 3 Chinese patents, and 3 Japanese patents are currently under consideration.\r\n\r\nRecently he has also published an extensive scholarly chapter in an internet open access book on 'Integrating Microphotonic Systems and MOEMS into standard Silicon CMOS Integrated circuitry”.\r\n\r\nFurthermore, Professor Snyman recently steered a new initiative at the TUT by introducing a 'Laboratory for Innovative Electronic Systems ' at the Department of Electrical Engineering. The model of this laboratory or center is to primarily combine outputs as achieved by high-level research with lower-level system development and entrepreneurship in a technical university environment. Students are allocated to projects at different levels with PhDs and Master students allocated to the generation of new knowledge and new technologies, while students at the diploma and Baccalaureus level are allocated to electronic systems development with a direct and a near application for application in industry or the commercial and public sectors in South Africa.\r\n\r\nProfessor Snyman received the WIRSAM Award of 1983 and the WIRSAM Award in 1985 in South Africa for best research papers by a young scientist at two international conferences on electron microscopy in South Africa. He subsequently received the SA Microelectronics Award for the best dissertation emanating from studies executed at a South African university in the field of Physics and Microelectronics in South Africa in 1987. In October of 2011, Professor Snyman received the prestigious Institutional Award for 'Innovator of the Year” for 2010 at the Tshwane University of Technology, South Africa. This award was based on the number of patents recognized and granted by local and international institutions as well as for his contributions concerning innovation at the TUT.",institutionString:null,institution:{name:"University of South Africa",country:{name:"South Africa"}}},{id:"317279",title:"Mr.",name:"Ali",middleName:"Usama",surname:"Syed",slug:"ali-syed",fullName:"Ali Syed",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/317279/images/16024_n.png",biography:"A creative, talented, and innovative young professional who is dedicated, well organized, and capable research fellow with two years of experience in graduate-level research, published in engineering journals and book, with related expertise in Bio-robotics, equally passionate about the aesthetics of the mechanical and electronic system, obtained expertise in the use of MS Office, MATLAB, SolidWorks, LabVIEW, Proteus, Fusion 360, having a grasp on python, C++ and assembly language, possess proven ability in acquiring research grants, previous appointments with social and educational societies with experience in administration, current affiliations with IEEE and Web of Science, a confident presenter at conferences and teacher in classrooms, able to explain complex information to audiences of all levels.",institutionString:null,institution:{name:"Air University",country:{name:"Pakistan"}}},{id:"75526",title:"Ph.D.",name:"Zihni Onur",middleName:null,surname:"Uygun",slug:"zihni-onur-uygun",fullName:"Zihni Onur Uygun",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/75526/images/12_n.jpg",biography:"My undergraduate education and my Master of Science educations at Ege University and at Çanakkale Onsekiz Mart University have given me a firm foundation in Biochemistry, Analytical Chemistry, Biosensors, Bioelectronics, Physical Chemistry and Medicine. After obtaining my degree as a MSc in analytical chemistry, I started working as a research assistant in Ege University Medical Faculty in 2014. In parallel, I enrolled to the MSc program at the Department of Medical Biochemistry at Ege University to gain deeper knowledge on medical and biochemical sciences as well as clinical chemistry in 2014. In my PhD I deeply researched on biosensors and bioelectronics and finished in 2020. Now I have eleven SCI-Expanded Index published papers, 6 international book chapters, referee assignments for different SCIE journals, one international patent pending, several international awards, projects and bursaries. In parallel to my research assistant position at Ege University Medical Faculty, Department of Medical Biochemistry, in April 2016, I also founded a Start-Up Company (Denosens Biotechnology LTD) by the support of The Scientific and Technological Research Council of Turkey. Currently, I am also working as a CEO in Denosens Biotechnology. The main purposes of the company, which carries out R&D as a research center, are to develop new generation biosensors and sensors for both point-of-care diagnostics; such as glucose, lactate, cholesterol and cancer biomarker detections. My specific experimental and instrumental skills are Biochemistry, Biosensor, Analytical Chemistry, Electrochemistry, Mobile phone based point-of-care diagnostic device, POCTs and Patient interface designs, HPLC, Tandem Mass Spectrometry, Spectrophotometry, ELISA.",institutionString:null,institution:{name:"Ege University",country:{name:"Turkey"}}},{id:"246502",title:"Dr.",name:"Jaya T.",middleName:"T",surname:"Varkey",slug:"jaya-t.-varkey",fullName:"Jaya T. Varkey",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/246502/images/11160_n.jpg",biography:"Jaya T. Varkey, PhD, graduated with a degree in Chemistry from Cochin University of Science and Technology, Kerala, India. She obtained a PhD in Chemistry from the School of Chemical Sciences, Mahatma Gandhi University, Kerala, India, and completed a post-doctoral fellowship at the University of Minnesota, USA. She is a research guide at Mahatma Gandhi University and Associate Professor in Chemistry, St. Teresa’s College, Kochi, Kerala, India.\nDr. Varkey received a National Young Scientist award from the Indian Science Congress (1995), a UGC Research award (2016–2018), an Indian National Science Academy (INSA) Visiting Scientist award (2018–2019), and a Best Innovative Faculty award from the All India Association for Christian Higher Education (AIACHE) (2019). She Hashas received the Sr. Mary Cecil prize for best research paper three times. She was also awarded a start-up to develop a tea bag water filter. \nDr. Varkey has published two international books and twenty-seven international journal publications. She is an editorial board member for five international journals.",institutionString:"St. Teresa’s College",institution:null},{id:"250668",title:"Dr.",name:"Ali",middleName:null,surname:"Nabipour Chakoli",slug:"ali-nabipour-chakoli",fullName:"Ali Nabipour Chakoli",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/250668/images/system/250668.jpg",biography:"Academic Qualification:\r\n•\tPhD in Materials Physics and Chemistry, From: Sep. 2006, to: Sep. 2010, School of Materials Science and Engineering, Harbin Institute of Technology, Thesis: Structure and Shape Memory Effect of Functionalized MWCNTs/poly (L-lactide-co-ε-caprolactone) Nanocomposites. Supervisor: Prof. Wei Cai,\r\n•\tM.Sc in Applied Physics, From: 1996, to: 1998, Faculty of Physics & Nuclear Science, Amirkabir Uni. of Technology, Tehran, Iran, Thesis: Determination of Boron in Micro alloy Steels with solid state nuclear track detectors by neutron induced auto radiography, Supervisors: Dr. M. Hosseini Ashrafi and Dr. A. Hosseini.\r\n•\tB.Sc. in Applied Physics, From: 1991, to: 1996, Faculty of Physics & Nuclear Science, Amirkabir Uni. of Technology, Tehran, Iran, Thesis: Design of shielding for Am-Be neutron sources for In Vivo neutron activation analysis, Supervisor: Dr. M. Hosseini Ashrafi.\r\n\r\nResearch Experiences:\r\n1.\tNanomaterials, Carbon Nanotubes, Graphene: Synthesis, Functionalization and Characterization,\r\n2.\tMWCNTs/Polymer Composites: Fabrication and Characterization, \r\n3.\tShape Memory Polymers, Biodegradable Polymers, ORC, Collagen,\r\n4.\tMaterials Analysis and Characterizations: TEM, SEM, XPS, FT-IR, Raman, DSC, DMA, TGA, XRD, GPC, Fluoroscopy, \r\n5.\tInteraction of Radiation with Mater, Nuclear Safety and Security, NDT(RT),\r\n6.\tRadiation Detectors, Calibration (SSDL),\r\n7.\tCompleted IAEA e-learning Courses:\r\nNuclear Security (15 Modules),\r\nNuclear Safety:\r\nTSA 2: Regulatory Protection in Occupational Exposure,\r\nTips & Tricks: Radiation Protection in Radiography,\r\nSafety and Quality in Radiotherapy,\r\nCourse on Sealed Radioactive Sources,\r\nCourse on Fundamentals of Environmental Remediation,\r\nCourse on Planning for Environmental Remediation,\r\nKnowledge Management Orientation Course,\r\nFood Irradiation - Technology, Applications and Good Practices,\r\nEmployment:\r\nFrom 2010 to now: Academic staff, Nuclear Science and Technology Research Institute, Kargar Shomali, Tehran, Iran, P.O. Box: 14395-836.\r\nFrom 1997 to 2006: Expert of Materials Analysis and Characterization. Research Center of Agriculture and Medicine. Rajaeeshahr, Karaj, Iran, P. O. Box: 31585-498.",institutionString:"Atomic Energy Organization of Iran",institution:{name:"Atomic Energy Organization of Iran",country:{name:"Iran"}}},{id:"248279",title:"Dr.",name:"Monika",middleName:"Elzbieta",surname:"Machoy",slug:"monika-machoy",fullName:"Monika Machoy",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/248279/images/system/248279.jpeg",biography:"Monika Elżbieta Machoy, MD, graduated with distinction from the Faculty of Medicine and Dentistry at the Pomeranian Medical University in 2009, defended her PhD thesis with summa cum laude in 2016 and is currently employed as a researcher at the Department of Orthodontics of the Pomeranian Medical University. She expanded her professional knowledge during a one-year scholarship program at the Ernst Moritz Arndt University in Greifswald, Germany and during a three-year internship at the Technical University in Dresden, Germany. She has been a speaker at numerous orthodontic conferences, among others, American Association of Orthodontics, European Orthodontic Symposium and numerous conferences of the Polish Orthodontic Society. She conducts research focusing on the effect of orthodontic treatment on dental and periodontal tissues and the causes of pain in orthodontic patients.",institutionString:"Pomeranian Medical University",institution:{name:"Pomeranian Medical University",country:{name:"Poland"}}},{id:"252743",title:"Prof.",name:"Aswini",middleName:"Kumar",surname:"Kar",slug:"aswini-kar",fullName:"Aswini Kar",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/252743/images/10381_n.jpg",biography:"uploaded in cv",institutionString:null,institution:{name:"KIIT University",country:{name:"India"}}},{id:"204256",title:"Dr.",name:"Anil",middleName:"Kumar",surname:"Kumar Sahu",slug:"anil-kumar-sahu",fullName:"Anil Kumar Sahu",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/204256/images/14201_n.jpg",biography:"I have nearly 11 years of research and teaching experience. I have done my master degree from University Institute of Pharmacy, Pt. Ravi Shankar Shukla University, Raipur, Chhattisgarh India. I have published 16 review and research articles in international and national journals and published 4 chapters in IntechOpen, the world’s leading publisher of Open access books. I have presented many papers at national and international conferences. I have received research award from Indian Drug Manufacturers Association in year 2015. My research interest extends from novel lymphatic drug delivery systems, oral delivery system for herbal bioactive to formulation optimization.",institutionString:null,institution:{name:"Chhattisgarh Swami Vivekanand Technical University",country:{name:"India"}}},{id:"253468",title:"Dr.",name:"Mariusz",middleName:null,surname:"Marzec",slug:"mariusz-marzec",fullName:"Mariusz Marzec",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/253468/images/system/253468.png",biography:"An assistant professor at Department of Biomedical Computer Systems, at Institute of Computer Science, Silesian University in Katowice. Scientific interests: computer analysis and processing of images, biomedical images, databases and programming languages. He is an author and co-author of scientific publications covering analysis and processing of biomedical images and development of database systems.",institutionString:"University of Silesia",institution:null},{id:"212432",title:"Prof.",name:"Hadi",middleName:null,surname:"Mohammadi",slug:"hadi-mohammadi",fullName:"Hadi Mohammadi",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/212432/images/system/212432.jpeg",biography:"Dr. Hadi Mohammadi is a biomedical engineer with hands-on experience in the design and development of many engineering structures and medical devices through various projects that he has been involved in over the past twenty years. Dr. Mohammadi received his BSc. and MSc. degrees in Mechanical Engineering from Sharif University of Technology, Tehran, Iran, and his PhD. degree in Biomedical Engineering (biomaterials) from the University of Western Ontario. He was a postdoctoral trainee for almost four years at University of Calgary and Harvard Medical School. He is an industry innovator having created the technology to produce lifelike synthetic platforms that can be used for the simulation of almost all cardiovascular reconstructive surgeries. He’s been heavily involved in the design and development of cardiovascular devices and technology for the past 10 years. He is currently an Assistant Professor with the University of British Colombia, Canada.",institutionString:"University of British Columbia",institution:{name:"University of British Columbia",country:{name:"Canada"}}},{id:"254463",title:"Prof.",name:"Haisheng",middleName:null,surname:"Yang",slug:"haisheng-yang",fullName:"Haisheng Yang",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/254463/images/system/254463.jpeg",biography:"Haisheng Yang, Ph.D., Professor and Director of the Department of Biomedical Engineering, College of Life Science and Bioengineering, Beijing University of Technology. He received his Ph.D. degree in Mechanics/Biomechanics from Harbin Institute of Technology (jointly with University of California, Berkeley). Afterwards, he worked as a Postdoctoral Research Associate in the Purdue Musculoskeletal Biology and Mechanics Lab at the Department of Basic Medical Sciences, Purdue University, USA. He also conducted research in the Research Centre of Shriners Hospitals for Children-Canada at McGill University, Canada. Dr. Yang has over 10 years research experience in orthopaedic biomechanics and mechanobiology of bone adaptation and regeneration. He earned an award from Beijing Overseas Talents Aggregation program in 2017 and serves as Beijing Distinguished Professor.",institutionString:"Beijing University of Technology",institution:null},{id:"255757",title:"Dr.",name:"Igor",middleName:"Victorovich",surname:"Lakhno",slug:"igor-lakhno",fullName:"Igor Lakhno",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/255757/images/system/255757.jpg",biography:"Lakhno Igor Victorovich was born in 1971 in Kharkiv (Ukraine). \nMD – 1994, Kharkiv National Medical Univesity.\nOb&Gyn; – 1997, master courses in Kharkiv Medical Academy of Postgraduate Education.\nPhD – 1999, Kharkiv National Medical Univesity.\nDSc – 2019, PL Shupik National Academy of Postgraduate Education \nLakhno Igor has been graduated from an international training courses on reproductive medicine and family planning held in Debrecen University (Hungary) in 1997. Since 1998 Lakhno Igor has worked as an associate professor of the department of obstetrics and gynecology of VN Karazin National University and an associate professor of the perinatology, obstetrics and gynecology department of Kharkiv Medical Academy of Postgraduate Education. Since June 2019 he’s a professor of the department of obstetrics and gynecology of VN Karazin National University and a professor of the perinatology, obstetrics and gynecology department of Kharkiv Medical Academy of Postgraduate Education . He’s an author of about 200 printed works and there are 17 of them in Scopus or Web of Science databases. Lakhno Igor is a rewiever of Journal of Obstetrics and Gynaecology (Taylor and Francis), Informatics in Medicine Unlocked (Elsevier), The Journal of Obstetrics and Gynecology Research (Wiley), Endocrine, Metabolic & Immune Disorders-Drug Targets (Bentham Open), The Open Biomedical Engineering Journal (Bentham Open), etc. He’s defended a dissertation for DSc degree \\'Pre-eclampsia: prediction, prevention and treatment”. Lakhno Igor has participated as a speaker in several international conferences and congresses (International Conference on Biological Oscillations April 10th-14th 2016, Lancaster, UK, The 9th conference of the European Study Group on Cardiovascular Oscillations). His main scientific interests: obstetrics, women’s health, fetal medicine, cardiovascular medicine.",institutionString:"V.N. Karazin Kharkiv National University",institution:{name:"Kharkiv Medical Academy of Postgraduate Education",country:{name:"Ukraine"}}},{id:"89721",title:"Dr.",name:"Mehmet",middleName:"Cuneyt",surname:"Ozmen",slug:"mehmet-ozmen",fullName:"Mehmet Ozmen",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/89721/images/7289_n.jpg",biography:null,institutionString:null,institution:{name:"Gazi University",country:{name:"Turkey"}}},{id:"243698",title:"M.D.",name:"Xiaogang",middleName:null,surname:"Wang",slug:"xiaogang-wang",fullName:"Xiaogang Wang",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/243698/images/system/243698.png",biography:"Dr. Xiaogang Wang, a faculty member of Shanxi Eye Hospital specializing in the treatment of cataract and retinal disease and a tutor for postgraduate students of Shanxi Medical University, worked in the COOL Lab as an international visiting scholar under the supervision of Dr. David Huang and Yali Jia from October 2012 through November 2013. Dr. Wang earned an MD from Shanxi Medical University and a Ph.D. from Shanghai Jiao Tong University. Dr. Wang was awarded two research project grants focused on multimodal optical coherence tomography imaging and deep learning in cataract and retinal disease, from the National Natural Science Foundation of China. He has published around 30 peer-reviewed journal papers and four book chapters and co-edited one book.",institutionString:"Shanxi Eye Hospital",institution:{name:"Shanxi Eye Hospital",country:{name:"China"}}},{id:"242893",title:"Ph.D. Student",name:"Joaquim",middleName:null,surname:"De Moura",slug:"joaquim-de-moura",fullName:"Joaquim De Moura",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/242893/images/7133_n.jpg",biography:"Joaquim de Moura received his degree in Computer Engineering in 2014 from the University of A Coruña (Spain). In 2016, he received his M.Sc degree in Computer Engineering from the same university. He is currently pursuing his Ph.D degree in Computer Science in a collaborative project between ophthalmology centers in Galicia and the University of A Coruña. His research interests include computer vision, machine learning algorithms and analysis and medical imaging processing of various kinds.",institutionString:null,institution:{name:"University of A Coruña",country:{name:"Spain"}}},{id:"267434",title:"Dr.",name:"Rohit",middleName:null,surname:"Raja",slug:"rohit-raja",fullName:"Rohit Raja",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRZkkQAG/Profile_Picture_2022-05-09T12:55:18.jpg",biography:null,institutionString:null,institution:null},{id:"294334",title:"B.Sc.",name:"Marc",middleName:null,surname:"Bruggeman",slug:"marc-bruggeman",fullName:"Marc Bruggeman",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/294334/images/8242_n.jpg",biography:"Chemical engineer graduate, with a passion for material science and specific interest in polymers - their near infinite applications intrigue me. \n\nI plan to continue my scientific career in the field of polymeric biomaterials as I am fascinated by intelligent, bioactive and biomimetic materials for use in both consumer and medical applications.",institutionString:null,institution:null},{id:"244950",title:"Dr.",name:"Salvatore",middleName:null,surname:"Di Lauro",slug:"salvatore-di-lauro",fullName:"Salvatore Di Lauro",position:null,profilePictureURL:"https://intech-files.s3.amazonaws.com/0030O00002bSF1HQAW/ProfilePicture%202021-12-20%2014%3A54%3A14.482",biography:"Name:\n\tSALVATORE DI LAURO\nAddress:\n\tHospital Clínico Universitario Valladolid\nAvda Ramón y Cajal 3\n47005, Valladolid\nSpain\nPhone number: \nFax\nE-mail:\n\t+34 983420000 ext 292\n+34 983420084\nsadilauro@live.it\nDate and place of Birth:\nID Number\nMedical Licence \nLanguages\t09-05-1985. Villaricca (Italy)\n\nY1281863H\n474707061\nItalian (native language)\nSpanish (read, written, spoken)\nEnglish (read, written, spoken)\nPortuguese (read, spoken)\nFrench (read)\n\t\t\nCurrent position (title and company)\tDate (Year)\nVitreo-Retinal consultant in ophthalmology. Hospital Clinico Universitario Valladolid. Sacyl. National Health System.\nVitreo-Retinal consultant in ophthalmology. Instituto Oftalmologico Recoletas. Red Hospitalaria Recoletas. Private practise.\t2017-today\n\n2019-today\n\t\n\t\nEducation (High school, university and postgraduate training > 3 months)\tDate (Year)\nDegree in Medicine and Surgery. University of Neaples 'Federico II”\nResident in Opthalmology. Hospital Clinico Universitario Valladolid\nMaster in Vitreo-Retina. IOBA. University of Valladolid\nFellow of the European Board of Ophthalmology. Paris\nMaster in Research in Ophthalmology. University of Valladolid\t2003-2009\n2012-2016\n2016-2017\n2016\n2012-2013\n\t\nEmployments (company and positions)\tDate (Year)\nResident in Ophthalmology. Hospital Clinico Universitario Valladolid. Sacyl.\nFellow in Vitreo-Retina. IOBA. University of Valladolid\nVitreo-Retinal consultant in ophthalmology. Hospital Clinico Universitario Valladolid. Sacyl. National Health System.\nVitreo-Retinal consultant in ophthalmology. Instituto Oftalmologico Recoletas. Red Hospitalaria Recoletas. \n\t2012-2016\n2016-2017\n2017-today\n\n2019-Today\n\n\n\t\nClinical Research Experience (tasks and role)\tDate (Year)\nAssociated investigator\n\n' FIS PI20/00740: DESARROLLO DE UNA CALCULADORA DE RIESGO DE\nAPARICION DE RETINOPATIA DIABETICA BASADA EN TECNICAS DE IMAGEN MULTIMODAL EN PACIENTES DIABETICOS TIPO 1. Grant by: Ministerio de Ciencia e Innovacion \n\n' (BIO/VA23/14) Estudio clínico multicéntrico y prospectivo para validar dos\nbiomarcadores ubicados en los genes p53 y MDM2 en la predicción de los resultados funcionales de la cirugía del desprendimiento de retina regmatógeno. Grant by: Gerencia Regional de Salud de la Junta de Castilla y León.\n' Estudio multicéntrico, aleatorizado, con enmascaramiento doble, en 2 grupos\nparalelos y de 52 semanas de duración para comparar la eficacia, seguridad e inmunogenicidad de SOK583A1 respecto a Eylea® en pacientes con degeneración macular neovascular asociada a la edad' (CSOK583A12301; N.EUDRA: 2019-004838-41; FASE III). Grant by Hexal AG\n\n' Estudio de fase III, aleatorizado, doble ciego, con grupos paralelos, multicéntrico para comparar la eficacia y la seguridad de QL1205 frente a Lucentis® en pacientes con degeneración macular neovascular asociada a la edad. (EUDRACT: 2018-004486-13). Grant by Qilu Pharmaceutical Co\n\n' Estudio NEUTON: Ensayo clinico en fase IV para evaluar la eficacia de aflibercept en pacientes Naive con Edema MacUlar secundario a Oclusion de Vena CenTral de la Retina (OVCR) en regimen de tratamientO iNdividualizado Treat and Extend (TAE)”, (2014-000975-21). Grant by Fundacion Retinaplus\n\n' Evaluación de la seguridad y bioactividad de anillos de tensión capsular en conejo. Proyecto Procusens. Grant by AJL, S.A.\n\n'Estudio epidemiológico, prospectivo, multicéntrico y abierto\\npara valorar la frecuencia de la conjuntivitis adenovírica diagnosticada mediante el test AdenoPlus®\\nTest en pacientes enfermos de conjuntivitis aguda”\\n. National, multicenter study. Grant by: NICOX.\n\nEuropean multicentric trial: 'Evaluation of clinical outcomes following the use of Systane Hydration in patients with dry eye”. Study Phase 4. Grant by: Alcon Labs'\n\nVLPs Injection and Activation in a Rabbit Model of Uveal Melanoma. Grant by Aura Bioscience\n\nUpdating and characterization of a rabbit model of uveal melanoma. Grant by Aura Bioscience\n\nEnsayo clínico en fase IV para evaluar las variantes genéticas de la vía del VEGF como biomarcadores de eficacia del tratamiento con aflibercept en pacientes con degeneración macular asociada a la edad (DMAE) neovascular. Estudio BIOIMAGE. IMO-AFLI-2013-01\n\nEstudio In-Eye:Ensayo clínico en fase IV, abierto, aleatorizado, de 2 brazos,\nmulticçentrico y de 12 meses de duración, para evaluar la eficacia y seguridad de un régimen de PRN flexible individualizado de 'esperar y extender' versus un régimen PRN según criterios de estabilización mediante evaluaciones mensuales de inyecciones intravítreas de ranibizumab 0,5 mg en pacientes naive con neovascularización coriodea secunaria a la degeneración macular relacionada con la edad. CP: CRFB002AES03T\n\nTREND: Estudio Fase IIIb multicéntrico, randomizado, de 12 meses de\nseguimiento con evaluador de la agudeza visual enmascarado, para evaluar la eficacia y la seguridad de ranibizumab 0.5mg en un régimen de tratar y extender comparado con un régimen mensual, en pacientes con degeneración macular neovascular asociada a la edad. CP: CRFB002A2411 Código Eudra CT:\n2013-002626-23\n\n\n\nPublications\t\n\n2021\n\n\n\n\n2015\n\n\n\n\n2021\n\n\n\n\n\n2021\n\n\n\n\n2015\n\n\n\n\n2015\n\n\n2014\n\n\n\n\n2015-16\n\n\n\n2015\n\n\n2014\n\n\n2014\n\n\n\n\n2014\n\n\n\n\n\n\n\n2014\n\nJose Carlos Pastor; Jimena Rojas; Salvador Pastor-Idoate; Salvatore Di Lauro; Lucia Gonzalez-Buendia; Santiago Delgado-Tirado. Proliferative vitreoretinopathy: A new concept of disease pathogenesis and practical\nconsequences. Progress in Retinal and Eye Research. 51, pp. 125 - 155. 03/2016. DOI: 10.1016/j.preteyeres.2015.07.005\n\n\nLabrador-Velandia S; Alonso-Alonso ML; Di Lauro S; García-Gutierrez MT; Srivastava GK; Pastor JC; Fernandez-Bueno I. Mesenchymal stem cells provide paracrine neuroprotective resources that delay degeneration of co-cultured organotypic neuroretinal cultures.Experimental Eye Research. 185, 17/05/2019. DOI: 10.1016/j.exer.2019.05.011\n\nSalvatore Di Lauro; Maria Teresa Garcia Gutierrez; Ivan Fernandez Bueno. Quantification of pigment epithelium-derived factor (PEDF) in an ex vivo coculture of retinal pigment epithelium cells and neuroretina.\nJournal of Allbiosolution. 2019. ISSN 2605-3535\n\nSonia Labrador Velandia; Salvatore Di Lauro; Alonso-Alonso ML; Tabera Bartolomé S; Srivastava GK; Pastor JC; Fernandez-Bueno I. Biocompatibility of intravitreal injection of human mesenchymal stem cells in immunocompetent rabbits. Graefe's archive for clinical and experimental ophthalmology. 256 - 1, pp. 125 - 134. 01/2018. DOI: 10.1007/s00417-017-3842-3\n\n\nSalvatore Di Lauro, David Rodriguez-Crespo, Manuel J Gayoso, Maria T Garcia-Gutierrez, J Carlos Pastor, Girish K Srivastava, Ivan Fernandez-Bueno. A novel coculture model of porcine central neuroretina explants and retinal pigment epithelium cells. Molecular Vision. 2016 - 22, pp. 243 - 253. 01/2016.\n\nSalvatore Di Lauro. Classifications for Proliferative Vitreoretinopathy ({PVR}): An Analysis of Their Use in Publications over the Last 15 Years. Journal of Ophthalmology. 2016, pp. 1 - 6. 01/2016. DOI: 10.1155/2016/7807596\n\nSalvatore Di Lauro; Rosa Maria Coco; Rosa Maria Sanabria; Enrique Rodriguez de la Rua; Jose Carlos Pastor. Loss of Visual Acuity after Successful Surgery for Macula-On Rhegmatogenous Retinal Detachment in a Prospective Multicentre Study. Journal of Ophthalmology. 2015:821864, 2015. DOI: 10.1155/2015/821864\n\nIvan Fernandez-Bueno; Salvatore Di Lauro; Ivan Alvarez; Jose Carlos Lopez; Maria Teresa Garcia-Gutierrez; Itziar Fernandez; Eva Larra; Jose Carlos Pastor. Safety and Biocompatibility of a New High-Density Polyethylene-Based\nSpherical Integrated Porous Orbital Implant: An Experimental Study in Rabbits. Journal of Ophthalmology. 2015:904096, 2015. DOI: 10.1155/2015/904096\n\nPastor JC; Pastor-Idoate S; Rodríguez-Hernandez I; Rojas J; Fernandez I; Gonzalez-Buendia L; Di Lauro S; Gonzalez-Sarmiento R. Genetics of PVR and RD. Ophthalmologica. 232 - Suppl 1, pp. 28 - 29. 2014\n\nRodriguez-Crespo D; Di Lauro S; Singh AK; Garcia-Gutierrez MT; Garrosa M; Pastor JC; Fernandez-Bueno I; Srivastava GK. Triple-layered mixed co-culture model of RPE cells with neuroretina for evaluating the neuroprotective effects of adipose-MSCs. Cell Tissue Res. 358 - 3, pp. 705 - 716. 2014.\nDOI: 10.1007/s00441-014-1987-5\n\nCarlo De Werra; Salvatore Condurro; Salvatore Tramontano; Mario Perone; Ivana Donzelli; Salvatore Di Lauro; Massimo Di Giuseppe; Rosa Di Micco; Annalisa Pascariello; Antonio Pastore; Giorgio Diamantis; Giuseppe Galloro. Hydatid disease of the liver: thirty years of surgical experience.Chirurgia italiana. 59 - 5, pp. 611 - 636.\n(Italia): 2007. ISSN 0009-4773\n\nChapters in books\n\t\n' Salvador Pastor Idoate; Salvatore Di Lauro; Jose Carlos Pastor Jimeno. PVR: Pathogenesis, Histopathology and Classification. Proliferative Vitreoretinopathy with Small Gauge Vitrectomy. Springer, 2018. ISBN 978-3-319-78445-8\nDOI: 10.1007/978-3-319-78446-5_2. \n\n' Salvatore Di Lauro; Maria Isabel Lopez Galvez. Quistes vítreos en una mujer joven. Problemas diagnósticos en patología retinocoroidea. Sociedad Española de Retina-Vitreo. 2018.\n\n' Salvatore Di Lauro; Salvador Pastor Idoate; Jose Carlos Pastor Jimeno. iOCT in PVR management. OCT Applications in Opthalmology. pp. 1 - 8. INTECH, 2018. DOI: 10.5772/intechopen.78774.\n\n' Rosa Coco Martin; Salvatore Di Lauro; Salvador Pastor Idoate; Jose Carlos Pastor. amponadores, manipuladores y tinciones en la cirugía del traumatismo ocular.Trauma Ocular. Ponencia de la SEO 2018..\n\n' LOPEZ GALVEZ; DI LAURO; CRESPO. OCT angiografia y complicaciones retinianas de la diabetes. PONENCIA SEO 2021, CAPITULO 20. (España): 2021.\n\n' Múltiples desprendimientos neurosensoriales bilaterales en paciente joven. Enfermedades Degenerativas De Retina Y Coroides. SERV 04/2016. \n' González-Buendía L; Di Lauro S; Pastor-Idoate S; Pastor Jimeno JC. Vitreorretinopatía proliferante (VRP) e inflamación: LA INFLAMACIÓN in «INMUNOMODULADORES Y ANTIINFLAMATORIOS: MÁS ALLÁ DE LOS CORTICOIDES. 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David Pan",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bSEI9QAO/Profile_Picture_1623656213532",institutionString:null,institution:{name:"University of Alabama in Huntsville",institutionURL:null,country:{name:"United States of America"}}},{id:"72920",title:"Prof.",name:"Yves",middleName:"Philippe",surname:"Rybarczyk",fullName:"Yves Rybarczyk",profilePictureURL:"https://mts.intechopen.com/storage/users/72920/images/system/72920.jpeg",institutionString:"Dalarna University, Faculty of Data and Information Sciences",institution:{name:"Dalarna University",institutionURL:null,country:{name:"Sweden"}}}]},{id:"27",title:"Multi-Agent Systems",keywords:"Collaborative Intelligence, Learning, Distributed Control System, Swarm Robotics, Decision Science, Software Engineering",scope:"Multi-agent systems are recognised as a state of the art field in Artificial Intelligence studies, which is popular due to the usefulness in facilitation capabilities to handle real-world problem-solving in a distributed fashion. The area covers many techniques that offer solutions to emerging problems in robotics and enterprise-level software systems. Collaborative intelligence is highly and effectively achieved with multi-agent systems. Areas of application include swarms of robots, flocks of UAVs, collaborative software management. Given the level of technological enhancements, the popularity of machine learning in use has opened a new chapter in multi-agent studies alongside the practical challenges and long-lasting collaboration issues in the field. It has increased the urgency and the need for further studies in this field. We welcome chapters presenting research on the many applications of multi-agent studies including, but not limited to, the following key areas: machine learning for multi-agent systems; modeling swarms robots and flocks of UAVs with multi-agent systems; decision science and multi-agent systems; software engineering for and with multi-agent systems; tools and technologies of multi-agent systems.",annualVolume:11423,isOpenForSubmission:!0,coverUrl:"https://cdn.intechopen.com/series_topics/covers/27.jpg",editor:{id:"148497",title:"Dr.",name:"Mehmet",middleName:"Emin",surname:"Aydin",fullName:"Mehmet Aydin",profilePictureURL:"https://mts.intechopen.com/storage/users/148497/images/system/148497.jpg",institutionString:null,institution:{name:"University of the West of England",institutionURL:null,country:{name:"United Kingdom"}}},editorTwo:null,editorThree:null,editorialBoard:[{id:"275140",title:"Dr.",name:"Dinh Hoa",middleName:null,surname:"Nguyen",fullName:"Dinh Hoa Nguyen",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRbnKQAS/Profile_Picture_1622204093453",institutionString:null,institution:{name:"Kyushu University",institutionURL:null,country:{name:"Japan"}}},{id:"20259",title:"Dr.",name:"Hongbin",middleName:null,surname:"Ma",fullName:"Hongbin Ma",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRhDJQA0/Profile_Picture_2022-05-02T08:25:21.jpg",institutionString:null,institution:{name:"Beijing Institute of Technology",institutionURL:null,country:{name:"China"}}},{id:"28640",title:"Prof.",name:"Yasushi",middleName:null,surname:"Kambayashi",fullName:"Yasushi Kambayashi",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002aYOQxQAO/Profile_Picture_1625660525470",institutionString:null,institution:{name:"Nippon Institute of Technology",institutionURL:null,country:{name:"Japan"}}}]}]}},libraryRecommendation:{success:null,errors:{},institutions:[]},route:{name:"profile.detail",path:"/profiles/429419",hash:"",query:{},params:{id:"429419"},fullPath:"/profiles/429419",meta:{},from:{name:null,path:"/",hash:"",query:{},params:{},fullPath:"/",meta:{}}}},function(){var e;(e=document.currentScript||document.scripts[document.scripts.length-1]).parentNode.removeChild(e)}()