These books synthesize perspectives of renowned scientists from the world’s most prestigious institutions - from Fukushima Renewable Energy Institute in Japan to Stanford University in the United States, including Columbia University (US), University of Sidney (AU), University of Miami (USA), Cardiff University (UK), and many others.
\\n\\n
This collaboration embodied the true essence of Open Access by simplifying the approach to OA publishing for Academic editors and authors who contributed their research and allowed the new research to be made available free and open to anyone anywhere in the world.
\\n\\n
To celebrate the 50 books published, we have gathered them at one location - just one click away, so that you can easily browse the subjects of your interest, download the content directly, share it or read online.
IntechOpen and Knowledge Unlatched formed a partnership to support researchers working in engineering sciences by enabling an easier approach to publishing Open Access content. Using the Knowledge Unlatched crowdfunding model to raise the publishing costs through libraries around the world, Open Access Publishing Fee (OAPF) was not required from the authors.
\n\n
Initially, the partnership supported engineering research, but it soon grew to include physical and life sciences, attracting more researchers to the advantages of Open Access publishing.
\n\n\n\n
These books synthesize perspectives of renowned scientists from the world’s most prestigious institutions - from Fukushima Renewable Energy Institute in Japan to Stanford University in the United States, including Columbia University (US), University of Sidney (AU), University of Miami (USA), Cardiff University (UK), and many others.
\n\n
This collaboration embodied the true essence of Open Access by simplifying the approach to OA publishing for Academic editors and authors who contributed their research and allowed the new research to be made available free and open to anyone anywhere in the world.
\n\n
To celebrate the 50 books published, we have gathered them at one location - just one click away, so that you can easily browse the subjects of your interest, download the content directly, share it or read online.
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1. Introduction
Urinary tract infection (UTI) is a topical problem of the contemporary pediatrics, pediatric nephrology, and urology, which takes the second or third place in the structure of children’s morbidity [1, 6, 9, 12]. Risk factors of UTI in children include neonatal period, in particular premature birth, family medical history, abnormalities of urinary tract development, disruptions of urodynamics, including vesicoureteric reflux, obstructive uropathy, neurogenic bladder malfunction, urolithiasis, constipation, fecal and perianal colonization, immunocompromising conditions, including AIDS, immunosuppression therapy, frequent bladder catheterization, diabetes, sexual activity in teenagers [1, 14, 15, 18]. Newborns and infants are under high risk of UTI development, among other factors, since their immune system is not yet sufficiently developed [7]. Among the many factors, which affect development and forecast of UTI, biological properties of microorganisms, which inhabit kidney tissue, are of no small importance [10, 22, 35, 43].
Etiological structure of UTI in children has changed recently. Many studies show the growing etiological significance of Enterococcus faecalis [1, 6, 8, 20, 37]. Clinical significance of enterococci, which were earlier considered in different saprophytes, is being reassessed currently [4, 10].
Increase in etiological significance of enterococci is caused by many reasons, including development of antibiotic resistance, in particular, resistance to cephalosporin, widely used for treating UTIs in children [8]. Enterococci can initiate the infectious process due to their genes coding many pathogenicity factors involved in adhesion and invasion processes, development of biofilms, and hystodamaging effect (Esp, Asa1, EfaA, CylA, CylM, GelE, FsrB, etc.) [6, 13, 16, 30, 35, 42]. It is known that virulence of microorganisms depends upon the quantity of these genes [17]. Scientists from Europe, England, America, India, and Japan are doing research in this area [21, 33, 38, 39, 41]. No research related to characteristics of intraspecific genetic variability, including pathogenicity factors, in uropathogenic E. faecalis isolates from children having UTI, has been done in Russia until now. All the above has been determined in the topic of the research, which was undertaken in the present paper.
The aim of this research was to identify genetic variability and phenotypic features of biologic properties in uropathogenic E. faecalis isolates from children having urinary tract infections to assess its virulent potential and clinical significance.
2. Materials and methods
At the first stage, bacterial tests of urine samples (n = 6438) isolated from patients in cases of UTI at the multispecialty regional children’s hospital from 2008 to 2016 were analyzed. Patients were aged from 3 days to 17 years.
At the second stage, biological properties and genetic variability of enterococci were studied. E. faecalis (n = 71) were isolated from urine of children with urinary tract infections and age from 3 days to 17 years in the diagnostic titer from 104 CFU/ml and higher during from 2013 to 2016. E. faecalis NCTC 12697 was used as the typical culture. All strains of uropathogenic E. faecalis were previously investigated using classical microbiological methods [22, 43]. Antimicrobial susceptibility was performed using disk diffusion method on the Muller-Hinton agar according to EUCAST.
Bacterial DNA of E. faecalis was isolated using DNA-express kit (Lytech, Russia). The testing of enterococci (n = 31) pathogenicity genes was made by polymerase chain reaction (PCR), using previously developed primer sets (Table 1) [5, 11, 32, 34], synthesized by Eurogen (Russia), on TProfessional 96 (Biometra, Germany). The amplification products were analyzed in a 1% agarose gel containing 1 μg/ml ethidium bromide in ultraviolet light using BioDocAnalyze (Biometra, Germany).
The obtained data were processed using the parametric analysis method. From the indicators of descriptive statistics, the relative values (P, %), their errors (mp, %) were calculated. To evaluate the degree of interrelation, the Pearson correlation analysis (R) was performed with calculation of correlation coefficient (r) and reliability of correlation (p). At statistical processing of the received materials, the software package Statistica 10.0 is used in the operating environment Windows 2010.
The research was approved by Interdisciplinary Committee for Ethics of the Federal State Budgetary Educational Institution of Higher Education “Pacific State Medical University” of the Ministry of Healthcare of the Russian Federation (protocol no. 4, 26.12.2016).
3. Results
The most common uropathogen in children with UTI, who were treated at the multispecialty regional children’s hospital, is Escherichia coli, for which specific gravity equals from 33.92 ± 1.7 to 62.96 ± 1.2%. Most frequently, it was pointed out in outpatients (in 62.96 ± 1.2% cases); less frequently, it was found at departments for newborns (33.92 ± 1.7%). The second significant etiological factor of UTI in children was E. faecalis, for which specific gravity ranged from 16.14 to 32.5% [22]. At the same time, E. faecalis was the most important factor in development of UTIs at departments for newborns: it was found in 57.2% of cases (Figure 1). Frequency of identifying E. faecalis in newborns diagnosed with UTI ranged from 30.8 to 74.5% of cases over 9 years.
Figure 1.
The etiological structure of UTI in newborn.
The present study analyzed the features of phenotypic manifestations of biological properties, including antibiotic resistance of E. faecalis (n = 71) isolated from urine of children with UTI. All analyzed uropathogenic E. faecalis had typical properties—morphology (cocci or oval Gram-positive bacteria), biochemical activity against mannitol, methylene blue, its absence in rhamnose fermentation, variability in glucose, lactose, sucrose and 2,3,5-triphenyltetrazolium chloride (TTC), and lack of mobility and catalase (Table 2).
Most E. faecalis isolated from the urine of children with UTI had in vitro enzymatic activity associated with pathogenicity: hemolytic, proteolytic, lipolytic, and lecithinase. A capsule was found in 45.6 ± 6.6% of uropathogenic enterococci. An inverse relationship was established between the presence of a capsule in E. faecalis with α-type hemolysis (r = 0.3, p = 0.0001), fermentation of milk (r = 0.31, p = 0.00), and a positive correlation with the microorganism titer in the urine (r = 0.33, p = 0.0332).
A direct correlation was established among the lecithinase and DNAase activity of E. faecalis (r = 0.31, p = 0.0438), the manifestation of hemolytic activity (α- or β-type) in uropathogenic enterococci, and the presence of the gene gelE (r = 0.49; p˂0.05).
The capsule (р1–2˂0.001; р1–3˂0.01), milk fermentation (р1–2˂0.001; р1–3˂0.01), gelatinous (р1–2˂0.001, р1–3˂0.05), and lipolytic activity with respect to the tween 60 (р1–2˂0.01, р1–3˂0.01) was determined more often in E. faecalis, isolated from the urine of newborn children (group 1) with UTI, than in other age groups.
E. faecalis, isolated from children under 1 year (group 2), have hemolytic activity more often than other age groups (р2–1˂0.05, р2–3˂0.05), but expressed less gelatinous activity (р1–2˂0.001; р2–3˂0.001). In this group, lipolytic activity against Tween 60 was less pronounced, but the differences were significant only between groups 1 and 2 (p˂0.01).
E. faecalis isolated from patients older than 1 year (group 3) showed more lipolytic activity against Tween 80, compared to other age groups (p1–3˂0.001; p2–3˂0.01), gelatin’s fermentation was determined more often than in enterococci of the group 2 (p˂0.001). The hemolytic activity of enterococci in the group 3 differed little compared to the group of newborn children. The other biochemical properties associated with pathogenicity factors of E. faecalis strains of this age group were similar to enterococci isolated from children under 1 year of age (Table 2).
Assessment of antibiotics resistance revealed that all studied cultures of uropathogenic enterococci (n = 71) are sensitive to vancomycin and nitrofurantoin. We found that enterococci are highly resistant to erythromycin (77.1 ± 5.02%), tetracycline (73.2 ± 5.3%), fluoroquinolones of the II and the III generations (ciprofloxacin (55.1 ± 5.9%), norfloxacin (48.6 ± 9.9%), and levofloxacin (46.5 ± 5.9%)). Were identified E. faecalis cultures, which are resistant (20.1 ± 4.9%) and mid-resistant (8.9 ± 3.5%) to the reserve drug linezolid. More than half (59.2 ± 5.8%) of the studied cultures of uropathogenic enterococci were resistant to several antibiotics (four or more antimicrobial agents).
It was found that enterococci, which are sensitive to penicillins, were characterized with lipolytic, lecithinase, and hemolytic (β-type) activity in vitro. Enterococci cultures which are resistant to fluoroquinolones, fermented sucrose, had proteolytic activity, and did not break down lactose. E. faecalis, which are resistant to gentamicin and erythromycin, had a capsule. Furthermore, E. faecalis, which are resistant to linezolid and chloramphenicol, had gelatinase, lecithinase, and lipolytic activities, as compared to other cultures (these data were not published).
In that way, analysis of biological properties of E. faecalis, isolated from urine of children with UTI in Primorsky region, showed that overwhelming majority of cultures had typical properties. The mentioned variability of biochemical and fermentation activities of pathogenicity factors and resistance to antibiotics suggested a certain phenotypic heterogeneity of E. faecalis.
3.1. Molecular genetics typing of E. faecalis
E. faecalis was tested for genes, coding various pathogenicity factors, using PCR method. It was found that clinical strains (n = 30) of enterococci isolated from urine of children with UTI contained two and more studied pathogenicity genes. In this context, 27 out of 30 uropathogenic E. faecalis had four and more of the studied genes (Table 3).
Biological properties
Enzymatic activity of E. faecalis
Newborns (n = 21)
Children from 29 days to 1 year (n = 18)
Children over 1 year old (n = 21)
Number of examined cultures, n
Abs. (Р ± mр, %)
Number of examined cultures, n
Abs. (Р ± mр, %)
Number of examined cultures, n
Abs. (Р ± mр, %)
Reduction of TTC *■
19
18 (94.7 ± 5.1)
14
11 (78.6 ± 11.0)
21
20 (95.2 ± 4.7)
Reduction of methylene blue
19
19 (100)
14
14 (100)
21
21 (100)
Biochemical activity in relation to:
Mannitol
20
20 (100)
13
13 (100)
19
19 (100)
Glucose ▲
20
19 (95.0 ± 4.9)
13
12 (92.3 ± 7.4)
19
19 (100)
Lactose ▲
20
19 (95.0 ± 4.9)
13
12 (92.3 ± 7.4)
19
16 (84.2 ± 8.4)
Rhamnose
21
0
13
0
19
0
Sucrose
20
17 (85.0 ± 8.0)
13
11 (84.6 ± 10.0)
19
16 (84.2 ± 8.4)
Presence of a capsule *▲
21
12 (57.1 ± 10.8)
17
6 (35.3 ± 11.6)
19
8 (42.1 ± 11.3)
Proteolytic activity in relation to:
Milk *▲
21
18 (85.7 ± 7.6)
18
9 (50.0 ± 11.8)
21
10 (47.6 ± 10.9)
Gelatin *▲■
20
9 (45.0 ± 11.1)
17
2 (11.8 ± 7.8)
21
7 (33.3 ± 10.3)
Lecithinase activity
19
5 (26.3 ± 10.1)
15
4 (26.7 ± 11.4)
21
6 (28.6 ± 9.9)
Lipolytic activity in relation to:
Tween 20
17
14 (82.4 ± 9.2)
12
11 (91.7 ± 7.9)
18
17 (94.4 ± 5.4)
Tween 60 *▲
8
4 (50.0 ± 17.2)
6
1 (16.7 ± 15.2)
11
3 (27.3 ± 13.4)
Tween 80 ▲■
21
14 (66.7 ± 10.3)
17
12 (70.6 ± 11.0)
19
16 (84.2 ± 8.4)
Table 2.
Peculiarities of phenotypic manifestations biological properties of E. faecalis depending on the patient’s age.
Note: *, p < 0.05 between 1 and 2 groups; ▲, p < 0.05 between 1 and 3 groups; ■, p < 0.05 between groups 2 and 3; Abs., absolute.
Id
ST
Isolate
Genotype
Total number of genes
aggA
esp
cylA
efaA
eep
gelE
1787
ST116
PR042
+
+
+
+
+
+
6
1789
ST179
PRV 052
+
+
+
+
+
+
6
1791
ST179
PRV100
+
+
+
+
+
+
6
1790
ST179
PRV105
+
+
+
+
+
+
6
nd
PRV086
+
+
+
+
+
+
6
nd
PR 230
+
+
+
+
+
+
6
1788
ST179
PRU047
+
—
+
+
+
+
5
nd
PR 181
+
+
+
+
+
—
5
1781
ST16
PR050
+
+
+
+
+
—
5
1786
ST41
PRV049
+
+
—
+
+
+
5
nd
PRL079
+
+
—
+
+
+
5
nd
PRV080
+
+
—
+
+
+
5
1793
ST774
PRAs81
+
+
—
+
+
+
5
nd
PR 198
+
—
—
+
+
+
4
nd
PR 158
+
—
—
+
+
+
4
1780
ST6
PRV054
+
—
—
+
+
+
4
1779
ST6
PRN030
+
—
—
+
+
+
4
1795
ST774
PRA029
+
—
—
+
+
+
4
1792
ST774
PR51
+
—
—
+
+
+
4
1784
ST40
PR055
—
+
—
+
+
+
4
1785
ST40
PRA038
—
+
—
+
+
+
4
nd
PR 228
+
—
+
+
+
—
4
1782
ST16
PRV092
+
—
+
+
+
—
4
nd
PR 215
+
+
—
+
+
—
4
nd
PR 223
+
+
—
+
+
—
4
1794
ST774
PR040
+
+
—
+
+
—
4
nd
NCTC 12697
—
—
+
+
+
+
4
nd
PR 97
—
—
+
+
+
+
4
nd
PR 161
—
—
—
+
+
+
3
nd
PR 206
—
—
—
+
+
+
3
1783
ST21
PRV082
—
—
—
+
+
—
2
Table 3.
Genotypes of uropathogenic E. faecalis in Primorsky region.
Note: nd, not determined.
Eleven variants of genes combinations, which code pathogenicity factors of E. faecalis, were identified. The most common variants are (aggA-esp-cylA-efaA-eep-gelE) and (aggA, efaA, eep, gelE).
Multilocus sequence typing (MLST) divided 17 E. faecalis strains into eight (ST6, ST16, ST21, ST40, ST41, ST116, ST179, and ST774) sequence types (The results have not been published.) It was noticed that uropathogenic E. faecalis strains attributed to ST6, ST40, ST179, ST774, and ST116 are resistant to four and more groups of antimicrobial agents (Table 4).
Id
Isolate
ST
Phenotype of antibiotic resistance
Genotype
1785
PRA038
40
ТЕТ-ERT-AmG-CHL-LZD
esp-efaA-eep-gelE
1784
PR055
ТЕТ-ERT-AmG-CHL
1780
PRV054
6
ТЕТ-ERT-AmG-CHL-FLQ
aggA-efaA-eep-gelE
1779
PRN030
ТЕТ-ERT-AmG-CHL-FLQ
1788
PRU047
179
ТЕТ-ERT-AmG-LZD
aggA-cylA-efaA-eep-gelE
1789
PRV052
ТЕТ-ERT-AmG
aggA-esp-cylA-efaA-eep-gelE
1791
PRV100
ТЕТ-ERT-AmG-CHL
1790
PRV105
ERT-AmG-CHL-FLQ
1787
PR042
116
ТЕТ-ERT-AmG-CHL-LZD
1781
PR050
16
ТЕТ-ERT
aggA-esp-cylA-efaA-eep
1782
PRV092
ТЕТ-ERT-AmG
aggA-cylA-efaA-eep
1794
PR040
774
AmG-FX
aggA-esp-efaA-eep
1793
PRAs081
ТЕТ-ERT-PEN-AmG-FLQ
aggA-esp-efaA-eep-gelE
1795
PRA029
ТЕТ-ERT-PEN-FLQ
aggA-efaA-eep-gelE
1792
PR051
ТЕТ-ERT-AmG-FLQ
1786
PRV049
41
ТЕТ
aggA-esp-efaA-eep-gelE
1783
PRV082
21
FLQ
efaA-eep
Table 4.
Antibiotic resistance of uropathogenic E. faecalis depending on the sequence type.
The results demonstrate broad variability of the range of genes, which code pathogenicity factors and reveal sequence types with multiple resistances to antimicrobial agents among uropathogenic E. faecalis isolated from children with UTI in Primorsky region.
4. Discussion
Urinary tract infection is an inflammatory process in the organs of the urinary system without specifying the level of damage or growth of microorganisms in the urinary tract with possible development of local inflammatory changes. UTI refers to the factors that initiate the development of chronic kidney disease and depends on the age of the children [27]. Etiological multifactority is peculiarity of these infections. For a long time, commonly recognized pathogens of uroinfections are Gram-negative enterobacteria, among which Escherichia coli is prevalent [25, 26, 29, 31, 40, 44].
Interest to studying enterococci as participants of infectious diseases has increased in recent years. Our research has shown that E. faecalis are a common pathogen, which causes UTI in children, most often in newborns (from 30.8 up to 75% of cases). Perhaps this is not accidental, as according to some authors, this microorganism is detected in children from the first days of life and its amount exceeds the content of E. coli in the newborn period [19]. The reason for this microbial composition of urine appears to be functional immunodeficiency in this category of patients.
However, until now, the true etiological significance of these microorganisms in the development of the infectious process and unfavorable outcomes remains uncertain due to the ever-changing properties of E. faecalis. It is known that the ability of bacteria to affect the kidneys and urinary tract is determined not by one but by a complex of properties necessary for this process, that at the different stages of the infectious process in the urinary system organs from the microorganism, priority expression of certain pathogenetically significant traits and/or their combinations is required [6].
All analyzed uropathogenic E. faecalis had typical properties—morphology, biochemical activity against mannitol, methylene blue, its absence in rhamnose fermentation, variability in glucose, lactose, sucrose and 2,3,5-TTC, and lack of mobility and catalase.
At the same time, weak and delayed acid formation from lactose was in three cultures (only on the third day), and in 12 isolates during fermentation of sucrose (by day 10). The results obtained with respect to a number of carbohydrates differ from the literature data, as it is known that E. faecalis ferment lactose and sucrose, and in relation to other sugars can be variable (Berdzhi). Possibly, this is associated with the spread of certain E. faecalis biovars in the territory of Primorsky Krai or within a single multispecialty hospital.
According to results of research conducted in recent years, it was determined that enterococci produce many virulence factors, which conduce to development of the infectious process (hemolysin, gelatinase, enterococcal surface protein, aggregation substance, serine protease, capsule, etc.). The greatest number of virulence factors was found in E. faecalis isolated from urine. High proteolytic activity of E. faecalis (hydrolysis of gelatinase, casein, and collagen) causes toxic damage to tissues and conduce to cicatricial changes in kidney [6, 19, 43].
Moreover, the change in the properties of the microorganisms, causing the urinary tract infection, such as the development of resistance factors to antimicrobial drugs and the biofilm formation, makes it difficult to manage patients, especially with chronic persistent and often recurrent infection.
Our research confirmed high virulence properties of E. faecalis isolated from urine of patients with and their manifestations depending on the patient’s age. For example, there is a negative correlation between the proteolytic activity of E. faecalis and the age of the children (r = 0.28, р = 0.002). Most E. faecalis isolated from the urine of children with UTI had in vitro enzymatic activity associated with pathogenicity: hemolytic, proteolytic, lipolytic, and lecithinase.
Currently, lipase is referred to understudied factors of enterococcal persistence, although it is known that lipase may be a potential virulence factor of E. faecalis [12]. Among the studied enterococci isolated from children with UTI, lipolytic activity was determined in 85.0 ± 8.2% of the cultures (more often with respect to Tween 20 and Tween 80). Enterococcus cultures showed heterogeneity in proteolytic and hemolytic activity. A reliable direct correlation between the phenotypic manifestation of β-type hemolytic activity with hydrolysis of gelatin (r = 0.58, p = 0.0001) and lecithinase activity (r = 0.52, p = 0.0004) of this uropathogen has been established. This confirms the combined effect of these pathogenic factors at a certain stage of the inflammatory process. A relationship was established between the phenotypic manifestation of pathogenicity factors and the age of patients.
The most common properties of E. faecalis isolated from urine of newborn children were a capsule, proteolytic, and lipolytic (in relation to Tween 60) activity. Enterococci isolated from 1-year-old children with UTI most frequently were characterized with hemolytic activity. Lipolytic (in relation to Tween 80) activity was most frequently found in cultures isolated from patients older than 1 year. The prevalence of these virulence factors suggests that they are associated with virulence of this species in UTI. Such features of the manifestation of biological properties in vitro indicate the selection of etiologically significant E. faecalis at the level of the macroorganism.
Furthermore, connection between sensitivity to antimicrobial drugs of E. faecalis and its biological properties was identified. It was found that uropathogenic enterococci characterized with proteolytic activity are resistant to antibacterial agents with different action mechanisms. In the work, it was found that E. faecalis, resistant to linezolid and chloramphenicol drugs that suppress protein synthesis at the level of the 50S subunit of the bacterial ribosome, possess a high pathogenic potential. These results require further research in this direction.
Interesting data were obtained with regard to the sensitivity of uropathogenic E. faecalis to the reserve drug linezolid, recommended for treatment of infections caused by strains, which are resistant to vancomycin, aminoglycosides, and betalactams. In Primorsky Krai, E. faecalis cultures were found resistant and intermediate sensitivity to linezolid. However, in Russia in the period from 2005 to 2013, there were isolated single enterococcal strains with reduced sensitivity to linezolid.
This way, the mentioned variability of biochemical and fermentation activity of factors related to pathogenicity demonstrated phenotypic heterogeneity of enterococci and might have a certain diagnostic significance.
Pathogenicity factors of bacteria are genetically determined by properties which are localized in genome of microorganisms in the form of “pathogenicity islands” [17]. These genetic elements can contain various sets of virulence genes, which are important for the development of the enterococcal infectious process, including genes of antibiotics resistance [17, 24]. At the present stage, the association of antibiotic resistance of E. faecalis with pathogenic factors is actively studied. It is known that strains of enterococci resistant to ampicillin, ciprofloxacin, and gentamicin, but sensitive to vancomycin and nitrofurantoin have more pathogenicity factors (hemolysin, gelatinase, hyaluronidase, form biofilms) than vancomycin resistant [2, 28]. E. faecalis having the asa1 gene are more resistant to fluoroquinolones (norfloxacin, ciprofloxacin, and levofloxacin) than isolates lacking this gene. Resistance to ciprofloxacin is significantly higher in E. faecalis having the genes cylL and cylS than in strains with their absence [23, 28]. esp gene-positive E. faecalis are more resistant to doxycycline than esp gene-negative cultures [3]. Among the strains with multidrug resistance, a high prevalence of genes asa1 and esp was observed [23, 36].
The research implemented using PCR method enabled to characterize in greater detail the structure of E. faecalis population isolated from children with UTI from Primorsky region. In our research, significant variability in occurrence frequency of the studied genes was found. Two of them—efaA (coding the surface antigen A (EfaA), which initiates the infectious process) and eep (coding Eep protein, which conduces to formation of a biofilm, making it resistant to various biological stress factors) were found in all studied uropathogenic E. faecalis, which proves their involvement in certain stages of the infectious process.
MLST analysis conducted earlier revealed eight sequence types, five of which were characterized by multidrug resistant.
This way, clinically significant E. faecalis strains have a complex of virulent properties, which allow the bacteria to materialize their pathogenic potential on all stages of the inflammation process in urinary system. This makes further research of the listed factors in clinical E. faecalis necessary to estimate objectively the contribution of these properties of the agent into pathophysiologic mechanism of infectious and inflammatory diseases.
5. Conclusions
Important role in the etiology of UTI is played not only by Gram-negative bacteria of the Enterobacteriaceae family, but also by gram-positive E. faecalis, which are of paramount importance in the development of UTI in newborns.
E. faecalis, isolated from the urine of children with UTI, have a complex of pathogenicity factors necessary for the development of the inflammatory process and their prolonged persistence in the urinary tract. The relationship between E. faecalis pathogenicity factors and the age of patients was determined.
Uropathogenic E. faecalis possess a polyantibiotic resistance, which is associated with its biological properties and belonging to a particular sequence type.
A set of phenotypic manifestations of the biological properties of E. faecalis (the presence/absence of hemolytic, gelatinase, lecithinase, lipolytic activities) established in the study may determine its clinical significance and serve as an in vitro diagnostic marker of resistance of the studied uropathogen to certain groups of antibacterial drugs.
Acknowledgments
The research was supported with an internal grant from the university (registration No. AAAA-A18-118031390014-9).
Conflict of interest
The authors declare that there is no conflict of interests.
\n',keywords:"urinary tract infections, Enterococcus faecalis, virulent properties, phenotypes, genotypes, newborns",chapterPDFUrl:"https://cdn.intechopen.com/pdfs/63199.pdf",chapterXML:"https://mts.intechopen.com/source/xml/63199.xml",downloadPdfUrl:"/chapter/pdf-download/63199",previewPdfUrl:"/chapter/pdf-preview/63199",totalDownloads:572,totalViews:218,totalCrossrefCites:1,totalDimensionsCites:1,totalAltmetricsMentions:0,impactScore:1,impactScorePercentile:68,impactScoreQuartile:3,hasAltmetrics:0,dateSubmitted:"May 3rd 2018",dateReviewed:"July 25th 2018",datePrePublished:"November 5th 2018",datePublished:"February 13th 2019",dateFinished:"August 24th 2018",readingETA:"0",abstract:"Urinary tract infection (UTI) is a topical problem of the contemporary pediatrics, pediatric nephrology, and urology. E. faecalis isolated from urine of children was the most important factor in development of UTIs at departments for newborns in Primorsky region. The variability of biochemical and fermentation activities of pathogenicity factors and resistance to antibiotics suggested a phenotypic heterogeneity of E. faecalis. It was found that uropathogenic enterococci characterized with proteolytic activity are resistant to antibacterial agents with different action mechanisms. Clinical isolates of E. faecalis contained two and more studied pathogenicity genes. Eleven variants of genes combinations, which code pathogenicity factors of E. faecalis, were identified. Uropathogenic E. faecalis strains attributed to ST6, ST40, ST179, ST774, and ST116 are resistant to four and more groups of antimicrobial agents. Our research confirmed high virulence properties of E. faecalis isolated from urine of patients with and their manifestations depending on the patient’s age. Clinically significant E. faecalis strains have a complex of virulent properties, which allow the bacteria to materialize their pathogenic potential on all stages of the inflammation process in urinary system.",reviewType:"peer-reviewed",bibtexUrl:"/chapter/bibtex/63199",risUrl:"/chapter/ris/63199",book:{id:"7452",slug:"microbiology-of-urinary-tract-infections-microbial-agents-and-predisposing-factors"},signatures:"Zaitseva Elena Aleksandrovna, Komenkova Tatiana Sergeevna,\nMelnikova Elena Aleksandrovna, Shadrin Andrey Mikhailovich and\nLuchaninova Valentina Nikolaevna",authors:null,sections:[{id:"sec_1",title:"1. Introduction",level:"1"},{id:"sec_2",title:"2. Materials and methods",level:"1"},{id:"sec_3",title:"3. Results",level:"1"},{id:"sec_3_2",title:"3.1. Molecular genetics typing of E. faecalis",level:"2"},{id:"sec_5",title:"4. Discussion",level:"1"},{id:"sec_6",title:"5. Conclusions",level:"1"},{id:"sec_7",title:"Acknowledgments",level:"1"},{id:"sec_7",title:"Conflict of interest",level:"1"}],chapterReferences:[{id:"B1",body:'Alberici I, Bayazit AK, Drozdz D, et al. Pathogens causing urinary tract infections in infants: A European overview by the ESCAPE study group. European Journal of Pediatrics. 2015;174(6):783-790. DOI: 10.1007/s00431-014-2459-3'},{id:"B2",body:'Banerjee T, Anupurba S. Prevalence of virulence factors and drug resistance in clinical isolates of Enterococci: A study from North India. Journal of Pathogens. 2015:7. ID 692612. DOI: 10.1155/2015/692612'},{id:"B3",body:'Baylan O, Nazik H, Bektöre B, Citil BE. The relationship between antibiotic resistance and virulence factors in urinary Enterococcus isolates. Mikrobiyoloji Bülteni. 2011;45(3):430-445'},{id:"B4",body:'Beganovic M, Luther MK, Rice LB, Arias CA, Rybak MJ, et al. 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Correlation between biofilm formation and gelE, esp, and agg genes in Enterococcus spp. clinical isolates. Virulence. 2014;5(5):634-637. DOI: 10.4161/viru.28998'},{id:"B35",body:'Shankar N, Lockatell CV, Arto SB, Drachenberg C, Gilmore MS, Johnson DE. Role of Enterococcus faecalis surface protein Esp in the pathogenesis of ascending urinary tract infection. Infection and Immunity. 2001;69(7):4366-4372. DOI: 10.1128/IAI.69.7.4366-4372.2001'},{id:"B36",body:'Shahraki S, Nezhad Mousavi MR. Determination of virulence factors in clinical multidrug resistance Enterococci isolates at Southeast of Iran. Jundishapur Journal of Microbiology. 2017. DOI: 10.58.12/jim.45514'},{id:"B37",body:'Skepyan EN, Vasilevski IV, Toptun PD. Analysis of the spectrum of infections of the urinary tract and the characteristic their sensitivity to antimicrobial drugs the children’s outpatient. Medical Panorama. 2013;8:35-38'},{id:"B38",body:'Stereva T, Atanasova D, Savov E, Petrova G, Mitov I. Incidence of virulence determinants in clinical Enterococcus faecalis and Enterococcus faecium isolates collected in Bulgaria. The Brazilian Journal of Infectious Diseases. 2016;20(2):127-133. DOI: 10.1016/j.bjid.2015.11.011'},{id:"B39",body:'Strezelecki Y, Hryniewicz W, Sadowy E. Gelatinase-associated phenotypes and genotypes among clinical isolates of Enterococcus faecalis in Poland. Polish Journal of Microbiology. 2011;60(4):287-292'},{id:"B40",body:'Vasilyeva LI, Kogan MI, Gudima IA, Naboka YL. Etiological structure and antibiotic susceptibility of pathogens of acute non-obstructive pyelonephritis in children. Attending Physician. 2009;8:8-11'},{id:"B41",body:'Upadhyaya PM, Ravikumar KL, Umapathy BL. Review of virulence factors of enterococci: An emerging nosocomial pathogen. Indian Journal of Medical Microbiology. 2009;27(4):301-305. DOI: 10.4103/0255-0857.55437'},{id:"B42",body:'Upadhyaya PM, Umapathy BL, Ravikumar KL. Comparative study for the presence of enterococcal virulence factors gelatinase, hemolysin and biofilm among clinical and commensal isolates of Enterococcus faecalis. Journal of Laboratory Physicians. 2010;2(2):100-104. DOI: 10.4103/0974-2727.72159'},{id:"B43",body:'Zaitseva EA, Krukovich EV, Melnikova EA, Luchaninova VN, Komenkova TS, Vaysero NS. The role of pathogenicity factors of Enterococcus faecalis in the development of pyelonephritis in children. Pacific Medical Journal. 2017;2(68):58-61'},{id:"B44",body:'Zorc JJ, Kidoo DA, Shaw KN. Diagnosis and menagment of pediatric urinary tract infections. Clinical Microbiology Reviews. 2005;18:417-422'}],footnotes:[],contributors:[{corresp:"yes",contributorFullName:"Zaitseva Elena Aleksandrovna",address:"elza200707@mail.ru",affiliation:'
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1. Introduction
“To move precisely in nanoworld, you donot succeed by perfecting proven techniques”.- Handelsblat. [1] . As stated, the nano research requires newer methodologies and techniques to be worked out to succeed. The microtechnology to nanotechnology needs a factor of thousand for size reduction. Different methodologies exist to club cooperation between macro, micro and nano robots and analytical based FEM for static, modal, harmonic and transient analysis of structures. Clubbed with multiparametric optimization and neural networks, FEM had developed as an optimal solution to all complicated problems of engineering, science, technology, medicine and research. The “bottom up” technology of late twentieth century promises the use of robotics for micro/nano manipulation processing [1]. The revolution of computers had led to development of closed form solutions which are extremely difficult to be obtained for any engineering problems [2]. This urge leads to adopting any one of the numerical techniques. FEA had been one of the options of researchers and choice of the method depends on the familiarity of the users. FEM exploits the research methodologies using direct approach, variational approach, direct approach, energy approach, weighted residual approach, Isoparametric formulation, static condensation and nonlinear analysis [2]. Numerical approximations can be reached by differential equations and PDE for various mathematical and nano technology problems of applied physics. FEM explores checking the validity of analytical studies of nanotechnology. Moreover the unaffordable experimental setup of nanoresearch could be replaced with FEA software as observed by engineers and scientists working on the field [2].
2. Applications of FEM in nano research
2.1 Electrospun nanofibrous Mats under biaxial tension
The nanofibrous mats can be used as freestanding electrodes in energy storage devices. These mats have non uniform material properties [3] with each and every single fiber having nonlinear characteristics. The research came up with two macroscopic continuum models with uniform or oriented nanofibre distribution to exactly replicate the nanofibrous mats under biaxial tension. The mechanical response of electrospun SF/PCL nanofibrous mats was explored. The model simulated by FEM exposed the deformation of nanofibrous mats and the gradual damage mechanism along with the microstructure.
2.2 Carbon nanotube reinforced composite’s stress transfer
The improvement [4] in mechanical properties of hybrid composite system strongly depends on interfacial mechanical properties (interfacial stress transfer efficiency). The interfaces are zones of structural, compositional and property gradients. The width varies from single atom to micrometers. The properties of the composite depend on the surface of the single fiber and the resin used for bonding. The stress transfer of SWNT reinforced composite was studied using hybrid FEM approach. Three dimensional REV (representative elementary volume method) had been used along with Molecular Dynamics (MD) to exploit the stress transfer mechanism of CNT reinforced composite. The [4] effects of fiber volume fraction, interfacial stiffness and elastic modulus on the stress of the matrix were explored. The analytical models are difficult along with experiments in nanoscale as they are too expensive. Hence FEM simulated models were used to predict the stress transfer and found to be accurate on validation.
2.3 Formulation of 3D finite element implementation for adhesive contact at nanoscale
A research was carried out [5] for three dimensional nanoscale contact problems with strong adhesion. The contact description was based on Lennard Jonnes [5] description suitable to explain Vanderwaals attraction between interacting bodies. By incorporating the potential into nonlinear continuum mechanics, formulations had been arrived for surface force and body force. Based on these formulations, overall contact algorithm had been arrived using FEM. This model has an application in biomechanics as denoted by adhesion of gecko spatula [5]. Efficient FE formulation was arrived based on surface traction. The behavior of contact model described by SF formulation was more efficient than BF formulation for strong adhesive existence.
2.4 Finite element simulation of micromachining of nanosized silicon: carbide particle reinforced composite
The nanosized silicon – carbide – particle (SICp) reinforced aluminum matrix composite’s micromachining process had been studied [6] using finite element method. The parameters of cohesive zone model had been found from stress – displacement curves of the molecular dynamics (MD) simulation. The model represented exactly the random properties of silicon – carbide particle distribution and the interfacial bonding between SICp and matrix [6]. The mechanism of machining was analyzed as per chip morphology, stress variation, temperature and cutting force. The FE simulation projected the fact that SICp caused non uniform interaction between the tool and reinforcement. The deformation mechanics [6] led to inhomogeneous stress variation and irregular cutting force.
2.5 FE modeling of double walled CNT based sensor
The Carbon Nanotubes (CNTs) are widely used for designing nano sensors, nano resonators and actuators [7]. The mass sensing characteristics of defective Double Walled Carbon nanotubes (DWNTs) were studied using FEM. Various finite element simulations covering chiral, zigzag and arm chair nanotubes with cantilever and bridged conditions using molecular [7] structural dynamics approach. The defects have been subdivided in to 6 missing atoms (A type) and 24 (B type) missing atoms on the outer wall of DWNT. COMBIN 14 element had been used for simulation of defective DWNTs with weak Vanderwaals force. The study revealed the fact that the frequency of defective DWNT reduced with increase in chiral angle. Also the frequency reduced with increase in pinhole defects.
2.6 Perspective on nanotips
Numerous methods have been fabricated for developing ultra-sharp tips for scanning probe microscopy and [8] electron microscopy. It has been observed that the sharp end terminates with very single atom in field ion microscope (FIM). The last atom had been intended to form atomic channel of electrons in field emission mode which would self-collimate a coherent electron beam with an outstanding brightness. Hence nanotips are found to behave as a source of self-collimated electron or ion beam. In this research [8] the distribution of electric field in the vicinity of nanotip apex that holds the topmost single atom had been studied analytically and numerically. The tip base was found to dominate nano protrusions which enhance electric field. The study revealed that nanotips with broad bases produce even less field than modest tips at the same voltage. This pronounced the fact that the tip base accounts for high voltages needed at imaging threshold field.
2.7 Axial vibration of embedded love – Bishop nanorods
In this research, nonlocal free vibration of axial rods embedded in elastic medium had been studied using Love – Bishop rod theory with FEM. Constitutive modeling for rod formulation using kinematic relations and dynamic equilibrium had been analyzed. Equation [9] of motion and boundary conditions were obtained by varying total potential of nano rod and were solved using separation of variation. Frequency equations of 4 types of nanorods were obtained. Size dependent FEM formulation was synthesized based on weighted residual method. The four parameters mode number, non-local parameter, rod length, and slenderness ratio were used to study the frequency parameters of nanorods. The free vibration frequencies of the simple axial rods had been found to be higher than that of Love bishop rods. These were evaluated by frequencies of two rod theories in higher modes.
2.8 Bending analysis of embedded nano plates using FEM
Eringen’s nonlocal elasticity theory is capable to capture small length scale effect. Hence it is widely used to explore the mechanical behavior of nanostructures [10]. Instead of using differential form, the integral form should be used to avoid inconsistency in results. Arbitrary kernel functions are used for general form. The first order [10] shear deformation theory is used to model the nanoplates. The study evaluates the first order shear deformable embedded nanoplates for bending using Erignen’s nonlocal theory. Using FEM approach the maximum deflection of the structure was evaluated. The results pronounced that the clamped or simply supported boundary conditions provided same trend for the effects of non-local parameter on the bending analysis of nano plate for Erignen’s integral and differential formulation. Also the results proved that the elastic foundation increased the stiffness of the structure and decreased the influence of nonlocal parameter.
2.9 Stresses at bone: particle reinforced nano composite interface
The biomaterial should satisfy its bio functionality and biocompatibility. The tissue – implant interface plays a huge role in both the parameters. Nanobioceramics is a newer technology that had widened [11] range of biomedical and dental applications including increased bioactivity for tissue regeneration and engineering, drug and gene delivery, treatment of viral infections and implantable surface modified medical devices for [11] better hard and soft tissue attachments. FEA had been accepted for simulations in biomechanics for analyzing stresses and strains in dental implants and surrounding bone structures. The tissue engineering needs combining 3D scaffolds with living cells to deliver the much needed cells to damage sites in the human body. This scaffold should be capable of making cells to attach and multiply. Hence the design of scaffold is a challenging task which could be narrated by the finite element analysis. Also the nanotechnology had revolutionized nanobiomaterials, tissue engineering nano scaffold, nano – drug delivery and dental nanocomposites [11].
The development of micro/nanotechnology [12] had led to characterization of the mechanical properties at micro- and nano- scales. The nano indentation test has a diamond indenter to produce indentation load and the penetration depth from which load – penetration curve (P-h) is obtained. The P-h curve can be used to define mechanical properties including hardness, elastic modulus and toughness [12]. Only few studies had been reported on elastic–plastic property of brittle bulk ceramics. There are two methods to derive material properties from loading and unloading indentation curves. One of the curves involves the use of unloading curves and classical elastic solution of infinite half space [12]. This method is suitable for calculating the hardness and elastic modulus of materials. Another methodology involves producing loading and unloading response curves for various parameters through finite element modeling. Stress strain relations can be produced by using the nanoindentation experiment. The ultra-fine – grained Si2 N2O – Si3N4 had been produced by hot press sintering of amorphous nano – sized silicon nitride powders at 1600, 1650 and 1700 Deg Celsius with nanosized additives. After evaluating by nano identation through finite element formulation, the elastic modulus and P-h curve are obtained. A newer theoretical methodology for evaluating stress strain relation of brittle ceramic materials had been identified. Numerous coefficients in theoretical calculation formula had been found using calculation and simulation results.
2.11 Torsional statics and dynamics of circular nanostructures
Newer technologies for developing advanced materials [13] and structures are advancing towards a minute length scale (i.1., micro – or nano – scale). This is the root of nanotechnology. By reducing the size of the materials, the materials exhibit specific and interesting non classical mechanical, chemical and electrical properties. The classical continuum theories fail to replicate the minute length scale [13]. Hence explicitly new continuum mechanics /atomic dynamic simulation are required. Erignen developed Non local elasticity theory as one of the continuum models. In this research, the torsional static and dynamic nonlocal effects for circular nanostructures for concentrated and distributed torques were investigated based on nonlocal elasticity stress theory [13]. Variational energy principle is obtained to derive governing differential equation and strain energy and kinetic energy components are obtained. A new nonlocal finite element method (NL-FEM) had been developed to solve integral nonlocal equation. The statics and dynamics of nonlocal nanoshafts, nanorods, and nanotubes with various loads and boundary conditions revealed possible numerical solutions which were compared with analytical solutions.
2.12 Elastic properties of coiled CNT reinforced Nano composite
With the invention of Carbon nanotube with advanced material properties, various nano composites had been developed [14]. A new algorithmic representative volume element (RVE) and finite element method had been formulated to find the elastic properties of coiled Carbon NanoTubes (CCNT). The elastic properties had been explored with the respect to interphase, fiber volume fraction, orientation, number of coils, tube diameter, coil diameter and helix angle using FEM. The elastic moduli of the nanocomposites were found to decrease with increase in the number of coils. Also it had been found that SWNT offers better reinforcement when compared with CCNT reinforcement.
2.13 Elastic and fracture characteristics of graphene – silicon nanosheet composites
Graphene and its composites find application in various fields of aerospace, bio-electric sensors, bio engineering, electronics, energy technology, and lithium batteries due to appreciable electrical, mechanical and thermal properties. The single layer graphene sheets (SLG) needs an appropriate substrate which should not alter the properties of graphene. In this research, an efficient method was developed for evaluating nonlinear stress strain behavior and fracture strength of graphene – silicon nanosheet composites. Nonlinear finite element model [15] had been evolved to obtain constitutive model of the problem which are computed using molecular dynamics (MD) simulations. Graphene is modeled as multilinear elastic and silicon is simulated as isotropic material. Using this model nonlinear behavior of graphene, silicon and their stress strain curve including inflection point leading to failure had been arrived. The results of stress strain curves and elastic modulus and the critical stress of single layer graphene (SLG), silicon nanosheet and their composites with different thickness of silicon nanosheet agrees with that of the molecular dynamics [15].
2.14 Thermo electric bulk and nanostructured materials
Numerous analytical solutions had been formulated [16] for coupled nonlinear behavior of thermoelectric device in one dimension. These devices are used in refrigeration and energy harvesting. In this research, a nonlinear model of thermoelectricity was developed using finite element method. The simulated model takes in to account Seebeck, Peltier and Thomson effects [16]. The FEM is represented in potential variables i.e., voltage and temperature and solved using Newton method by formulating stiffness and Jacobian matrices. The results were verified with simulated one dimensional model. The FEM is then implemented to estimate energy conversion of nanostructured thermoelectric materials. Thus the advantages of nanostructured materials lie in the increased performance and miniaturization.
2.15 FEM of nano: indentation to characterize thin film coatings
Thin film coatings [17] are used in tribological, corrosion resistance of mechanical components, tooling, biomedical implants, electronics, microsystem packaging, cutting tool coatings and magnetic devices. The film coatings are explicitly used for reducing wear and tear. It is a current need to investigate the thin film coatings for critical loads that lead to ultimate fracture. Since nanoindentation is a nondestructive one, it is preferred and imperatively it could be simulated by finite element method (FEM). Using FEM, the hardness, elastic modulus, endurance loads, optimal thickness, optimal critical load, stress distribution and contact pressure between substrate and layer could be found.
2.16 Electric field gradients and bipolar electrochemistry effects
The effects of electric field in alternating (AC) and direct (DC)voltages had been explored in vivo and vitro with [18] electrodes in connection with tissues and implanted cells. The electro simulation through noncontact wireless settings by dipoles by bipolar chemistry is highly possible. FEM studies with same configuration that of experimental studies had proved that the voltage profiles are in qualitative agreement with known bipolar effects. There exist [18] a clear mapping of charge gradients at the material surface leading to growth of neurons. The insulating materials distort the electric space distribution while the dipole at the border of implanted conducting material extends along the material surface and much smoother in intercalation materials.
2.17 Elastic stability of curved nanobeam by finite element approach
The elastic stability of curved nanobeam had been investigated using Eringen’s strain driven model [19] coupled with higher order shear deformation theory. The influence of different structural theories and analyses of nanobeam is taken into account while deducing the model. The governing differential equation is solved by finite element method using 3 –noded curved beam. The model had been validated using analytical/numerical solutions. The parameters such as thickness ratio, beam length, rise of curved beam, boundary conditions and size dependent [19] or nonlocal are analyzed based on buckling behavior of curved nanobeams. The results prove that the type of buckling mode corresponding to lowest critical value would be varying based on geometrical and internal material length scale parameter and boundary conditions [19].
2.18 Tensile modulus of CNT reinforced polypropylene composite
The reinforcing efficiency of carbon nanotubes (CNTs) in polymers had been found using finite element modeling. The probability distribution functions [20] of CNT diameter, orientation, dispersion and waviness had been incorporated in the finite element model to derive how the CNT characteristics affect the tensile modulus of CNT reinforced polypropylene composite. The scanning electron microscopy images of CNT/PP composites made by melt mixing and injection molding had been used by image analysis approach [20]. The predicted model had been found to be experimentally correct as per ASTM D638.
3. Conclusion
The FE methods had been used to study thermo-electrical–mechanical coupled model. The integrity of lumped element, distributed element and system level element for design, modeling and simulation of nano/micro mechanical systems (N/MEMS) had been achieved by FEM. The nanostructures, nanocomposites and CNTs and their composites had been modeled using FEA. Further FEM had been applied in nanomaterials and systems used in medicine, dental science, biotechnology and electric field in the form of electrospinning.
The investigation of material properties with 1 – 100 nm dimensions had been achieved by nanoscience and technology. Thanks to nanotechnology and FEM, one of the dimensional materials such as CNTs, silica carbide nanotube, nanowire, nanorod and nanobeam had been dream of innovation. The field effective transistors, gas sensors, nanoactuators, nanocantilevers are the live examples. It has been found that these structures have varied applications in nano/micro – electro – mechanical systems (NEMS /MEMS). Ultra capacitors had been found application in hybrid cars. This chapter elaborated the applications of finite element method in varied applications of nanotechnology including CNTs, nano beams, nanorods, nanobiomaterials, graphene coated materials, nanosensors, nanotips and curved nanobeams. Apart from these applications the nanotechnology extends hands to day to day applications such as self-cleansing walls, wall claddings, reinforcement to cement matrix. The FEM could be extended to these materials which had not been extensively covered. A correct mechanical model simulated by finite element modeling would replicate the exact experimental setup and provide solutions to constitutive modeling and all engineering problems. The best example is the usage of CNTs as reinforcements in composites and cementitious materials. The CNTs are costlier that almost 85% of the CNT reinforcement is studies using FEA software by the researchers instead of experimental studies. The last few decades had been dedicated to CNTs, sensors, diagnostic probes and multifunctional materials based on CNTs, electronic devices and energy storage devices. The sensors could be used to monitor all kind of structures including cracks in bridges and structural collapses of civil engineering structures. The replacement of silicon based sensors and transistors with CNT based printed transistors are future challenge and already researches are on using FEM and FEA software. With advent of computers and FEA software such as ANSYS, ABAQUS, NASTRAN the unaffordable experimental analysis of nanoscience had been replaced with analytical studies using FEM.
Acknowledgments
I thank AAA College of Engineering and Technology, Sivakasi management and faculty who directly or indirectly contributed to this work. I thank my PhD guide Dr. S. Prabavathy, Mepco Schlenk Engineering College Sivakasi who is my inspiration for whatever I do.
\n',keywords:"nanotechnology, FEM, FEA, research, nanoscience",chapterPDFUrl:"https://cdn.intechopen.com/pdfs/73935.pdf",chapterXML:"https://mts.intechopen.com/source/xml/73935.xml",downloadPdfUrl:"/chapter/pdf-download/73935",previewPdfUrl:"/chapter/pdf-preview/73935",totalDownloads:326,totalViews:0,totalCrossrefCites:0,dateSubmitted:"June 15th 2020",dateReviewed:"October 22nd 2020",datePrePublished:"November 5th 2020",datePublished:"November 17th 2021",dateFinished:"November 5th 2020",readingETA:"0",abstract:"The Finite Element Analysis in the field of Nanotechnology is continually contributing to the areas ranging from electronics, micro computing, material science, quantum science, engineering, biotechnology, medicine, aerospace, and environment and in computational nanotechnology. The finite element method (FEM) is widely used for solving problems of traditional fields of engineering and Nano research where experimental analysis is unaffordable. This numerical technique can provide accurate solution to complex engineering problems. Over decades this method has become the noted research area for the mathematicians. The popularity of FEM is due to the advent of computer FEA software such as NASTRAN, ANSYS, ABAQUS, Matlab, OPEN Foam, Simscale and the like. With the development of nanoscience, the researchers found difficulties in spending funds for nano related projects. 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Conclusion",level:"1"},{id:"sec_22",title:"Acknowledgments",level:"1"}],chapterReferences:[{id:"B1",body:'Sarhan, M. (n.d.). Computational Finite Element Methods in Nanotechnology. CRC Press, 2013 630p'},{id:"B2",body:'Y.M. Desai, T.I. Eldo, A.H.Shah, Finite Element Method with Applications in Engineering, Pearson , 2011, 470p'},{id:"B3",body:'Yin, Y., & Xiong, J. Finite element analysis of electrospun nanofibrous mats under biaxial tension. Nanomaterials, 2018 8(5), 1-19. https://doi.org/10.3390/nano8050348'},{id:"B4",body:'Spanos, K. N., Georgantzinos, S. K., & Anifantis, N. K. . Investigation of stress transfer in carbon nanotube reinforced composites using a multi-scale finite element approach. Composites Part B: Engineering, 2014, 63, 85-93. https://doi.org/10.1016/j.compositesb.2014.03.020'},{id:"B5",body:'Sauer, R. A., & Wriggers, P. Formulation and analysis of a three-dimensional finite element implementation for adhesive contact at the nanoscale. Computer Methods in Applied Mechanics and Engineering, 2009, 198(49-52), 3871-3883. https://doi.org/10.1016/j.cma.2009.08.019'},{id:"B6",body:'Pen, H., Guo, J., Cao, Z., Wang, X., & Wang, Z. Finite element simulation of the micromachining of nanosized-silicon-carbide-particle reinforced composite materials based on the cohesive zone model. Nami Jishu Yu Jingmi Gongcheng/Nanotechnology and Precision Engineering, 2018, 1(4), 242-247. https://doi.org/10.1016/j.npe.2018.12.003'},{id:"B7",body:'Patel, A. M., & Joshi, A. Y. Atomistic Finite Element Modeling and Analysis of pinholes in Double Walled Carbon Nanotube based mass sensor. Materials Today: Proceedings, 2016, 3(6) 1438-1443. doi:10.1016/j.matpr.2016.04.026'},{id:"B8",body:'Rezeq, M. Finite element simulation and analytical analysis for nano field emission sources that terminate with a single atom: A new perspective on nanotips. Applied Surface Science, 2011, 258(5), 1750-1755. https://doi.org/10.1016/j.apsusc.2011.10.034'},{id:"B9",body:'Civalek, Ö., & Numanoğlu, H. M. Nonlocal finite element analysis for axial vibration of embedded love–bishop nanorods. International Journal of Mechanical Sciences, 2020, 188. https://doi.org/10.1016/j.ijmecsci.2020.105939'},{id:"B10",body:'Ansari, R., Torabi, J., & Norouzzadeh, A. Bending analysis of embedded nanoplates based on the integral formulation of Eringen’s nonlocal theory using the finite element method. Physica B: Condensed Matter, 2018, 534(January), 90-97. https://doi.org/10.1016/j.physb.2018.01.025'},{id:"B11",body:'Choi, A. H. Stress induced at the bone-particle-reinforced nanocomposite interface. In Interfaces in Particle and Fibre Reinforced Composites. Elsevier Ltd. 2020 https://doi.org/10.1016/b978-0-08-102665-6.00019-4'},{id:"B12",body:'Luo, J., Zhao, Z., Shen, J., & Zhang, C. Elastic-plastic analysis of ultrafine-grained Si2N 2O-Si3N4 composites by nanoindentation and finite element simulation. Ceramics International, 2014, 40(5), 7073-7080. https://doi.org/10.1016/j.ceramint.2013.12.039'},{id:"B13",body:'Lim, C. W., Islam, M. Z., & Zhang, G, A nonlocal finite element method for torsional statics and dynamics of circular nanostructures. International Journal of Mechanical Sciences, 2015, 94-95, 232-243. https://doi.org/10.1016/j.ijmecsci.2015.03.002'},{id:"B14",body:'Khani, N., Yildiz, M., & Koc, B., Elastic properties of coiled carbon nanotube reinforced nanocomposite: A finite element study. Materials and Design, 2016, 109, 123-132. https://doi.org/10.1016/j.matdes.2016.06.126'},{id:"B15",body:'Gangele, A., & Pandey, A. K., Elastic and fracture characteristics of graphene-silicon nanosheet composites using nonlinear finite element method. International Journal of Mechanical Sciences, 2018, 142-143(May), 491-501. https://doi.org/10.1016/j.ijmecsci.2018.05.012'},{id:"B16",body:'Potirniche, G. P., & Barannyk, L. L. A nonlinear finite element model for the performance of thermoelectric bulk and nanostructured materials., Energy, 2019, 185, 262-273. https://doi.org/10.1016/j.energy.2019.07.040'},{id:"B17",body:'Alaboodi, A. S., & Hussain, Z. Finite element modeling of nano-indentation technique to characterize thin film coatings. Journal of King Saud University - Engineering Sciences, 2019, 31(1), 61-69. https://doi.org/10.1016/j.jksues.2017.02.001'},{id:"B18",body:'Abad, L., Rajnicek, A. M., & Casañ-Pastor, N. Electric field gradients and bipolar electrochemistry effects on neural growth: A finite element study on immersed electroactive conducting electrode materials. Electrochimica Acta, 2019, 317, 102-111. https://doi.org/10.1016/j.electacta.2019.05.149'},{id:"B19",body:'Polit, O., Merzouki, T., & Ganapathi, M. Elastic stability of curved nanobeam based on higher-order shear deformation theory and nonlocal analysis by finite element approach. Finite Elements in Analysis and Design, 2018 146(April), 1-15. https://doi.org/10.1016/j.finel.2018.04.002'},{id:"B20",body:'Bhuiyan, M. A., Pucha, R. V., Worthy, J., Karevan, M., & Kalaitzidou, K. Understanding the effect of CNT characteristics on the tensile modulus of CNT reinforced polypropylene using finite element analysis. Computational Materials Science, 2013, 79, 368-376. https://doi.org/10.1016/j.commatsci.2013.06.046'}],footnotes:[],contributors:[{corresp:"yes",contributorFullName:"RameshBabu Chandran",address:"rameshbabu_1979@rediffmail.com",affiliation:'
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However, its function is not only fundamental in protection from infectious disease but also provides aberrant response in allergens such as with asthma and allergic rhinitis. Allergic diseases like asthma and allergic rhinitis are characterized by a distinct type of inflammatory response, driven by immunoglobulin E (IgE)-dependent mechanisms. In asthma and allergic rhinitis, the inflammatory response is mediated by interaction of several immune cells (monocytes, lymphocytes, and polymorphonuclear cells) and cellular chemical mediators. In particular, atopic allergic response leads to destruction of multiple target cells such as epithelial, parenchymal and vascular and connective tissue of the airways. In addition, in inflammatory response in asthma and allergic rhinitis, sensory nerves are sensitized, leading to clinical manifestations. Sneezing and coughing are hypersensitivity responses of sensory nerves in allergic rhinitis and asthma, respectively. Similarly, nasal congestion and discharge in allergic rhinitis are due to vasodilatation that leads to plasma exudates as well as mucous secretion. The allergic inflammatory response is regulated by several transcription factors, particularly nuclear factor-κb (NF-κB), GATA-3 protein 3, and GATA binding protein.",book:{id:"7062",slug:"rhinosinusitis",title:"Rhinosinusitis",fullTitle:"Rhinosinusitis"},signatures:"Andrew Kiboneka and Dan Kibuule",authors:[{id:"202850",title:"Mr.",name:"Dan",middleName:null,surname:"Kibuule",slug:"dan-kibuule",fullName:"Dan Kibuule"},{id:"280538",title:"Dr.",name:"Andrew",middleName:null,surname:"Kiboneka",slug:"andrew-kiboneka",fullName:"Andrew Kiboneka"}]},{id:"55472",doi:"10.5772/intechopen.69089",title:"Paranasal Sinus Anatomy: What the Surgeon Needs to Know",slug:"paranasal-sinus-anatomy-what-the-surgeon-needs-to-know",totalDownloads:5573,totalCrossrefCites:3,totalDimensionsCites:4,abstract:"Performing a smooth and clean sinus surgery goes hand in hand with a perfect understanding of the nasal and paranasal anatomy. Within this chapter, the paranasal and related structures surgical anatomy will be extensively reviewed, with emphasis on the anatomical landmarks and the normal anatomical variations, which have a significant impact on the function, pathology, and surgical procedures of the paranasal sinuses.",book:{id:"5911",slug:"paranasal-sinuses",title:"Paranasal Sinuses",fullTitle:"Paranasal Sinuses"},signatures:"Abdulmalik S. Alsaied",authors:[{id:"199716",title:"Dr.",name:"Abdulmalik",middleName:"Saad",surname:"Alsaied",slug:"abdulmalik-alsaied",fullName:"Abdulmalik Alsaied"}]},{id:"67186",doi:"10.5772/intechopen.86212",title:"Local Allergic Rhinitis: An Old Story but a New Entity",slug:"local-allergic-rhinitis-an-old-story-but-a-new-entity",totalDownloads:795,totalCrossrefCites:4,totalDimensionsCites:4,abstract:"Local allergic rhinitis (LAR) is a novel concept defining clinical allergic rhinitis with no evidence of systemic sensitization to aeroallergens. In this unique condition, the allergic response is confined to the nasal mucosa and can be demonstrated using different methods such as the immunoglobulin-E (IgE) level in the nasal secretions, nasal provocation test (NPT), or basophil activation test (BAT) with specific allergens or more sophisticated molecular diagnostic techniques. Furthermore, local allergic rhinitis can be relieved by interventions used to treat systemic allergic conditions such as antihistamines or anti-IgE monoclonal antibodies. Last but not least, several small studies demonstrated the efficacy of allergen immunotherapy for ameliorating LAR symptoms. In this chapter we reviewed old data and new concepts regarding clinical manifestation, plausible mechanisms, and treatments of LAR. The long-standing question whether LAR is an integral part of the “atopic spectrum” or it is a single-organ immune-mediated disease, is yet to be determined.",book:{id:"7062",slug:"rhinosinusitis",title:"Rhinosinusitis",fullTitle:"Rhinosinusitis"},signatures:"Ramit Maoz-Segal, Diti Machnes-Maayan, Irena Veksler-Offengenden, Shirly Frizinsky, Soad Hajyahia and Nancy Agmon-Levin",authors:[{id:"293794",title:"Dr.",name:"Ramit",middleName:null,surname:"Maoz-Segal",slug:"ramit-maoz-segal",fullName:"Ramit Maoz-Segal"},{id:"293823",title:"Dr.",name:"Diti",middleName:null,surname:"Machnes-Maayan",slug:"diti-machnes-maayan",fullName:"Diti Machnes-Maayan"},{id:"299506",title:"Dr.",name:"Irena",middleName:null,surname:"Veksler-Offengenden",slug:"irena-veksler-offengenden",fullName:"Irena Veksler-Offengenden"},{id:"299508",title:"Dr.",name:"Soad",middleName:null,surname:"Hajyahia",slug:"soad-hajyahia",fullName:"Soad Hajyahia"},{id:"299510",title:"Dr.",name:"Shirly",middleName:null,surname:"Frizinsky",slug:"shirly-frizinsky",fullName:"Shirly Frizinsky"},{id:"299511",title:"Prof.",name:"Nancy",middleName:null,surname:"Agmon-Levin",slug:"nancy-agmon-levin",fullName:"Nancy Agmon-Levin"}]}],mostDownloadedChaptersLast30Days:[{id:"63699",title:"Management of the Complications of Maxillary Sinus Augmentation",slug:"management-of-the-complications-of-maxillary-sinus-augmentation",totalDownloads:7747,totalCrossrefCites:1,totalDimensionsCites:3,abstract:"Dental implant rehabilitation of the posterior maxillary region has always been a challenging issue due to both alveolar ridge atrophy and sinus pneumatization. Maxillary sinus augmentation is a well-known and predictable procedure in vertical deficiencies of the posterior maxilla. To date, various techniques have been described based on the physiology of intrasinus bone repair to obtain better outcomes. Nevertheless, these procedures could also be associated with several intra- and postoperative complications such as perforation of the sinus membrane, hemorrhage, infection, graft resorption, and loss of the graft or implants. The aim of this chapter is to review the contemporary methods for maxillary sinus augmentation and to present both recommendations for prevention and management of the associated complications.",book:{id:"7245",slug:"challenging-issues-on-paranasal-sinuses",title:"Challenging Issues on Paranasal Sinuses",fullTitle:"Challenging Issues on Paranasal Sinuses"},signatures:"Alper Sindel, Mehmet Mustafa Özarslan and Öznur Özalp",authors:[{id:"244837",title:"Dr.",name:"Alper",middleName:null,surname:"Sindel",slug:"alper-sindel",fullName:"Alper Sindel"},{id:"244918",title:"Dr.",name:"Mehmet Mustafa",middleName:null,surname:"Özarslan",slug:"mehmet-mustafa-ozarslan",fullName:"Mehmet Mustafa Özarslan"},{id:"244919",title:"Ms.",name:"Öznur",middleName:null,surname:"Özalp",slug:"oznur-ozalp",fullName:"Öznur Özalp"}]},{id:"55472",title:"Paranasal Sinus Anatomy: What the Surgeon Needs to Know",slug:"paranasal-sinus-anatomy-what-the-surgeon-needs-to-know",totalDownloads:5569,totalCrossrefCites:3,totalDimensionsCites:4,abstract:"Performing a smooth and clean sinus surgery goes hand in hand with a perfect understanding of the nasal and paranasal anatomy. Within this chapter, the paranasal and related structures surgical anatomy will be extensively reviewed, with emphasis on the anatomical landmarks and the normal anatomical variations, which have a significant impact on the function, pathology, and surgical procedures of the paranasal sinuses.",book:{id:"5911",slug:"paranasal-sinuses",title:"Paranasal Sinuses",fullTitle:"Paranasal Sinuses"},signatures:"Abdulmalik S. Alsaied",authors:[{id:"199716",title:"Dr.",name:"Abdulmalik",middleName:"Saad",surname:"Alsaied",slug:"abdulmalik-alsaied",fullName:"Abdulmalik Alsaied"}]},{id:"69430",title:"Concurrent Rhinoplasty and Endoscopic Sinus Surgery",slug:"concurrent-rhinoplasty-and-endoscopic-sinus-surgery",totalDownloads:1150,totalCrossrefCites:0,totalDimensionsCites:0,abstract:"Combining rhinoplasty and endoscopic sinus surgery (ESS) was first reported in 1991 by Sheman and Matarasso. Since then, many authors have documented a large series showing the overall efficacy of combining the two procedures. The focus of this manuscript is to document the author’s recent experience with combining rhinoplasty and endoscopic sinus surgery and highlight the changes that have occurred during the author’s 2-years experience. A retrospective data review was performed on 53 (31 females and 22 men, age range 16–55 years) patients who underwent combined rhinoplasty and ESS between January 2016 and December 2018 at Pantai Hospital Kuala Lumpur by the same surgeon. The mean age was 31.8 years. All patients had severe nasal obstruction with chronic rhinosinusitis and were followed up for a minimum of 6 months post-surgery and underwent ENT workup, which included history, office rigid endoscopy, CT scans of paranasal sinuses and preoperative photography. Initially, the ESS was performed followed by the open rhinoplasty with or without osteotomy. The ESS consisted of middle turbinate reduction [15/53 (28.3%)], maxillary antrostomy [36/53 (67.9%)], ethmoidectomy [38/53 (71.6%)], frontal sinusotomy [7/53 (13.2%)], and sphenoidotomy [9/53 (16.9%)]. Most of the sinus symptoms resolved postoperatively with 47 (88.6%) of 53 patients describing their improvement as significant. Fifty (94.3%) of 53 patients stated that they would recommend the concurrent procedure. The benefits of these advances are illustrated by a review of the literature with good results (functional and cosmetic) and minimal complications.",book:{id:"7062",slug:"rhinosinusitis",title:"Rhinosinusitis",fullTitle:"Rhinosinusitis"},signatures:"Balwant Singh Gendeh",authors:[{id:"67669",title:null,name:"Balwant Singh",middleName:null,surname:"Gendeh",slug:"balwant-singh-gendeh",fullName:"Balwant Singh Gendeh"}]},{id:"62774",title:"Orbital Cellulitis",slug:"orbital-cellulitis-1",totalDownloads:1004,totalCrossrefCites:1,totalDimensionsCites:2,abstract:"Infection in the soft tissues of the orbit, posterior to the orbital septum results in orbital cellulitis. This is a very serious condition, which may occur as a complication of sinusitis by contiguous spread or may result from haematogenous spread or from trauma. Orbital cellulitis presents with periorbital swelling, proptosis, conjunctival chemosis and injection, extraocular motility deficits and visual loss. It requires comanagement by the ophthalmologist and otorhinolaryngologist when secondary to sinusitis. It is important that this condition is recognized early, and immediate management is done to prevent impending visual loss and further complications of periosteal abscesses, meningitis, cavernous sinus thrombosis and death. This chapter reviews the epidemiology of orbital cellulitis, pathogenesis, causative organisms, investigations (including imaging of the sinuses) and treatment. Prognostic factors and conditions that complicate this such as diabetes will also be discussed.",book:{id:"7245",slug:"challenging-issues-on-paranasal-sinuses",title:"Challenging Issues on Paranasal Sinuses",fullTitle:"Challenging Issues on Paranasal Sinuses"},signatures:"Lizette Mowatt",authors:[{id:"31774",title:"Dr.",name:"Lizette",middleName:null,surname:"Mowatt",slug:"lizette-mowatt",fullName:"Lizette Mowatt"}]},{id:"55606",title:"Maxillary Sinus Augmentation for Dental Implants",slug:"maxillary-sinus-augmentation-for-dental-implants",totalDownloads:2107,totalCrossrefCites:1,totalDimensionsCites:1,abstract:"Pneumatization of the maxillary sinus secondary to posterior maxillary tooth loss is an extremely common finding. Significant atrophy of the maxilla prevents dental implant placement in this region. Grafting the floor of the maxillary sinus has emerged as the most common surgical modality for correcting this inadequacy. Graft material is introduced into the space created inferior to the sinus membrane. Various grafting materials and techniques might be used in this procedure. The aim of this article is to review the essentials of maxillary sinus augmentation, clarify this procedure for otolaryngologists, explain its function, and describe the augmentation materials, techniques, and complications.",book:{id:"5911",slug:"paranasal-sinuses",title:"Paranasal Sinuses",fullTitle:"Paranasal Sinuses"},signatures:"Gökhan Göçmen and Yasar Özkan",authors:[{id:"200960",title:"Ph.D.",name:"Gokhan",middleName:null,surname:"Gocmen",slug:"gokhan-gocmen",fullName:"Gokhan Gocmen"},{id:"206912",title:"Prof.",name:"Yaşar",middleName:null,surname:"Özkan",slug:"yasar-ozkan",fullName:"Yaşar Özkan"}]}],onlineFirstChaptersFilter:{topicId:"1100",limit:6,offset:0},onlineFirstChaptersCollection:[],onlineFirstChaptersTotal:0},preDownload:{success:null,errors:{}},subscriptionForm:{success:null,errors:{}},aboutIntechopen:{},privacyPolicy:{},peerReviewing:{},howOpenAccessPublishingWithIntechopenWorks:{},sponsorshipBooks:{sponsorshipBooks:[],offset:0,limit:8,total:null},allSeries:{pteSeriesList:[],lsSeriesList:[],hsSeriesList:[],sshSeriesList:[],testimonialsList:[]},series:{item:{id:"7",title:"Biomedical Engineering",doi:"10.5772/intechopen.71985",issn:"2631-5343",scope:"Biomedical Engineering is one of the fastest-growing interdisciplinary branches of science and industry. The combination of electronics and computer science with biology and medicine has improved patient diagnosis, reduced rehabilitation time, and helped to facilitate a better quality of life. Nowadays, all medical imaging devices, medical instruments, or new laboratory techniques result from the cooperation of specialists in various fields. The series of Biomedical Engineering books covers such areas of knowledge as chemistry, physics, electronics, medicine, and biology. 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Dr. Koprowski has authored more than a hundred research papers with dozens in impact factor (IF) journals and has authored or co-authored six books. Additionally, he is the author of several national and international patents in the field of biomedical devices and imaging. 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Saxena",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRET3QAO/Profile_Picture_2022-05-10T10:10:26.jpeg",institutionString:"King George's Medical University",institution:{name:"King George's Medical University",institutionURL:null,country:{name:"India"}}}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null}]},subseriesFiltersForPublishedBooks:[{group:"subseries",caption:"Bacterial Infectious Diseases",value:3,count:2},{group:"subseries",caption:"Parasitic Infectious Diseases",value:5,count:4},{group:"subseries",caption:"Viral Infectious Diseases",value:6,count:7}],publicationYearFilters:[{group:"publicationYear",caption:"2022",value:2022,count:2},{group:"publicationYear",caption:"2021",value:2021,count:4},{group:"publicationYear",caption:"2020",value:2020,count:3},{group:"publicationYear",caption:"2019",value:2019,count:3},{group:"publicationYear",caption:"2018",value:2018,count:1}],authors:{paginationCount:249,paginationItems:[{id:"274452",title:"Dr.",name:"Yousif",middleName:"Mohamed",surname:"Abdallah",slug:"yousif-abdallah",fullName:"Yousif Abdallah",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/274452/images/8324_n.jpg",biography:"I certainly enjoyed my experience in Radiotherapy and Nuclear Medicine, particularly it has been in different institutions and hospitals with different Medical Cultures and allocated resources. Radiotherapy and Nuclear Medicine Technology has always been my aspiration and my life. As years passed I accumulated a tremendous amount of skills and knowledge in Radiotherapy and Nuclear Medicine, Conventional Radiology, Radiation Protection, Bioinformatics Technology, PACS, Image processing, clinically and lecturing that will enable me to provide a valuable service to the community as a Researcher and Consultant in this field. My method of translating this into day to day in clinical practice is non-exhaustible and my habit of exchanging knowledge and expertise with others in those fields is the code and secret of success.",institutionString:null,institution:{name:"Majmaah University",country:{name:"Saudi Arabia"}}},{id:"313277",title:"Dr.",name:"Bartłomiej",middleName:null,surname:"Płaczek",slug:"bartlomiej-placzek",fullName:"Bartłomiej Płaczek",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/313277/images/system/313277.jpg",biography:"Bartłomiej Płaczek, MSc (2002), Ph.D. (2005), Habilitation (2016), is a professor at the University of Silesia, Institute of Computer Science, Poland, and an expert from the National Centre for Research and Development. His research interests include sensor networks, smart sensors, intelligent systems, and image processing with applications in healthcare and medicine. He is the author or co-author of more than seventy papers in peer-reviewed journals and conferences as well as the co-author of several books. He serves as a reviewer for many scientific journals, international conferences, and research foundations. Since 2010, Dr. Placzek has been a reviewer of grants and projects (including EU projects) in the field of information technologies.",institutionString:"University of Silesia",institution:{name:"University of Silesia",country:{name:"Poland"}}},{id:"35000",title:"Prof.",name:"Ulrich H.P",middleName:"H.P.",surname:"Fischer",slug:"ulrich-h.p-fischer",fullName:"Ulrich H.P Fischer",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/35000/images/3052_n.jpg",biography:"Academic and Professional Background\nUlrich H. P. has Diploma and PhD degrees in Physics from the Free University Berlin, Germany. He has been working on research positions in the Heinrich-Hertz-Institute in Germany. Several international research projects has been performed with European partners from France, Netherlands, Norway and the UK. He is currently Professor of Communications Systems at the Harz University of Applied Sciences, Germany.\n\nPublications and Publishing\nHe has edited one book, a special interest book about ‘Optoelectronic Packaging’ (VDE, Berlin, Germany), and has published over 100 papers and is owner of several international patents for WDM over POF key elements.\n\nKey Research and Consulting Interests\nUlrich’s research activity has always been related to Spectroscopy and Optical Communications Technology. Specific current interests include the validation of complex instruments, and the application of VR technology to the development and testing of measurement systems. He has been reviewer for several publications of the Optical Society of America\\'s including Photonics Technology Letters and Applied Optics.\n\nPersonal Interests\nThese include motor cycling in a very relaxed manner and performing martial arts.",institutionString:null,institution:{name:"Charité",country:{name:"Germany"}}},{id:"341622",title:"Ph.D.",name:"Eduardo",middleName:null,surname:"Rojas Alvarez",slug:"eduardo-rojas-alvarez",fullName:"Eduardo Rojas Alvarez",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/341622/images/15892_n.jpg",biography:null,institutionString:null,institution:{name:"University of Cuenca",country:{name:"Ecuador"}}},{id:"215610",title:"Prof.",name:"Muhammad",middleName:null,surname:"Sarfraz",slug:"muhammad-sarfraz",fullName:"Muhammad Sarfraz",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/215610/images/system/215610.jpeg",biography:"Muhammad Sarfraz is a professor in the Department of Information Science, Kuwait University, Kuwait. His research interests include optimization, computer graphics, computer vision, image processing, machine learning, pattern recognition, soft computing, data science, and intelligent systems. Prof. Sarfraz has been a keynote/invited speaker at various platforms around the globe. He has advised/supervised more than 110 students for their MSc and Ph.D. theses. He has published more than 400 publications as books, journal articles, and conference papers. He has authored and/or edited around seventy books. Prof. Sarfraz is a member of various professional societies. He is a chair and member of international advisory committees and organizing committees of numerous international conferences. He is also an editor and editor in chief for various international journals.",institutionString:"Kuwait University",institution:{name:"Kuwait University",country:{name:"Kuwait"}}},{id:"32650",title:"Prof.",name:"Lukas",middleName:"Willem",surname:"Snyman",slug:"lukas-snyman",fullName:"Lukas Snyman",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/32650/images/4136_n.jpg",biography:"Lukas Willem Snyman received his basic education at primary and high schools in South Africa, Eastern Cape. He enrolled at today's Nelson Metropolitan University and graduated from this university with a BSc in Physics and Mathematics, B.Sc Honors in Physics, MSc in Semiconductor Physics, and a Ph.D. in Semiconductor Physics in 1987. After his studies, he chose an academic career and devoted his energy to the teaching of physics to first, second, and third-year students. After positions as a lecturer at the University of Port Elizabeth, he accepted a position as Associate Professor at the University of Pretoria, South Africa.\r\n\r\nIn 1992, he motivates the concept of 'television and computer-based education” as means to reach large student numbers with only the best of teaching expertise and publishes an article on the concept in the SA Journal of Higher Education of 1993 (and later in 2003). The University of Pretoria subsequently approved a series of test projects on the concept with outreach to Mamelodi and Eerste Rust in 1993. In 1994, the University established a 'Unit for Telematic Education ' as a support section for multiple faculties at the University of Pretoria. In subsequent years, the concept of 'telematic education” subsequently becomes well established in academic circles in South Africa, grew in popularity, and is adopted by many universities and colleges throughout South Africa as a medium of enhancing education and training, as a method to reaching out to far out communities, and as a means to enhance study from the home environment.\r\n\r\nProfessor Snyman in subsequent years pursued research in semiconductor physics, semiconductor devices, microelectronics, and optoelectronics.\r\n\r\nIn 2000 he joined the TUT as a full professor. Here served for a period as head of the Department of Electronic Engineering. Here he makes contributions to solar energy development, microwave and optoelectronic device development, silicon photonics, as well as contributions to new mobile telecommunication systems and network planning in SA.\r\n\r\nCurrently, he teaches electronics and telecommunications at the TUT to audiences ranging from first-year students to Ph.D. level.\r\n\r\nFor his research in the field of 'Silicon Photonics” since 1990, he has published (as author and co-author) about thirty internationally reviewed articles in scientific journals, contributed to more than forty international conferences, about 25 South African provisional patents (as inventor and co-inventor), 8 PCT international patent applications until now. Of these, two USA patents applications, two European Patents, two Korean patents, and ten SA patents have been granted. A further 4 USA patents, 5 European patents, 3 Korean patents, 3 Chinese patents, and 3 Japanese patents are currently under consideration.\r\n\r\nRecently he has also published an extensive scholarly chapter in an internet open access book on 'Integrating Microphotonic Systems and MOEMS into standard Silicon CMOS Integrated circuitry”.\r\n\r\nFurthermore, Professor Snyman recently steered a new initiative at the TUT by introducing a 'Laboratory for Innovative Electronic Systems ' at the Department of Electrical Engineering. The model of this laboratory or center is to primarily combine outputs as achieved by high-level research with lower-level system development and entrepreneurship in a technical university environment. Students are allocated to projects at different levels with PhDs and Master students allocated to the generation of new knowledge and new technologies, while students at the diploma and Baccalaureus level are allocated to electronic systems development with a direct and a near application for application in industry or the commercial and public sectors in South Africa.\r\n\r\nProfessor Snyman received the WIRSAM Award of 1983 and the WIRSAM Award in 1985 in South Africa for best research papers by a young scientist at two international conferences on electron microscopy in South Africa. He subsequently received the SA Microelectronics Award for the best dissertation emanating from studies executed at a South African university in the field of Physics and Microelectronics in South Africa in 1987. In October of 2011, Professor Snyman received the prestigious Institutional Award for 'Innovator of the Year” for 2010 at the Tshwane University of Technology, South Africa. This award was based on the number of patents recognized and granted by local and international institutions as well as for his contributions concerning innovation at the TUT.",institutionString:null,institution:{name:"University of South Africa",country:{name:"South Africa"}}},{id:"317279",title:"Mr.",name:"Ali",middleName:"Usama",surname:"Syed",slug:"ali-syed",fullName:"Ali Syed",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/317279/images/16024_n.png",biography:"A creative, talented, and innovative young professional who is dedicated, well organized, and capable research fellow with two years of experience in graduate-level research, published in engineering journals and book, with related expertise in Bio-robotics, equally passionate about the aesthetics of the mechanical and electronic system, obtained expertise in the use of MS Office, MATLAB, SolidWorks, LabVIEW, Proteus, Fusion 360, having a grasp on python, C++ and assembly language, possess proven ability in acquiring research grants, previous appointments with social and educational societies with experience in administration, current affiliations with IEEE and Web of Science, a confident presenter at conferences and teacher in classrooms, able to explain complex information to audiences of all levels.",institutionString:null,institution:{name:"Air University",country:{name:"Pakistan"}}},{id:"75526",title:"Ph.D.",name:"Zihni Onur",middleName:null,surname:"Uygun",slug:"zihni-onur-uygun",fullName:"Zihni Onur Uygun",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/75526/images/12_n.jpg",biography:"My undergraduate education and my Master of Science educations at Ege University and at Çanakkale Onsekiz Mart University have given me a firm foundation in Biochemistry, Analytical Chemistry, Biosensors, Bioelectronics, Physical Chemistry and Medicine. After obtaining my degree as a MSc in analytical chemistry, I started working as a research assistant in Ege University Medical Faculty in 2014. In parallel, I enrolled to the MSc program at the Department of Medical Biochemistry at Ege University to gain deeper knowledge on medical and biochemical sciences as well as clinical chemistry in 2014. In my PhD I deeply researched on biosensors and bioelectronics and finished in 2020. Now I have eleven SCI-Expanded Index published papers, 6 international book chapters, referee assignments for different SCIE journals, one international patent pending, several international awards, projects and bursaries. In parallel to my research assistant position at Ege University Medical Faculty, Department of Medical Biochemistry, in April 2016, I also founded a Start-Up Company (Denosens Biotechnology LTD) by the support of The Scientific and Technological Research Council of Turkey. Currently, I am also working as a CEO in Denosens Biotechnology. The main purposes of the company, which carries out R&D as a research center, are to develop new generation biosensors and sensors for both point-of-care diagnostics; such as glucose, lactate, cholesterol and cancer biomarker detections. My specific experimental and instrumental skills are Biochemistry, Biosensor, Analytical Chemistry, Electrochemistry, Mobile phone based point-of-care diagnostic device, POCTs and Patient interface designs, HPLC, Tandem Mass Spectrometry, Spectrophotometry, ELISA.",institutionString:null,institution:{name:"Ege University",country:{name:"Turkey"}}},{id:"246502",title:"Dr.",name:"Jaya T.",middleName:"T",surname:"Varkey",slug:"jaya-t.-varkey",fullName:"Jaya T. Varkey",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/246502/images/11160_n.jpg",biography:"Jaya T. Varkey, PhD, graduated with a degree in Chemistry from Cochin University of Science and Technology, Kerala, India. She obtained a PhD in Chemistry from the School of Chemical Sciences, Mahatma Gandhi University, Kerala, India, and completed a post-doctoral fellowship at the University of Minnesota, USA. She is a research guide at Mahatma Gandhi University and Associate Professor in Chemistry, St. Teresa’s College, Kochi, Kerala, India.\nDr. Varkey received a National Young Scientist award from the Indian Science Congress (1995), a UGC Research award (2016–2018), an Indian National Science Academy (INSA) Visiting Scientist award (2018–2019), and a Best Innovative Faculty award from the All India Association for Christian Higher Education (AIACHE) (2019). She Hashas received the Sr. Mary Cecil prize for best research paper three times. She was also awarded a start-up to develop a tea bag water filter. \nDr. Varkey has published two international books and twenty-seven international journal publications. She is an editorial board member for five international journals.",institutionString:"St. Teresa’s College",institution:null},{id:"250668",title:"Dr.",name:"Ali",middleName:null,surname:"Nabipour Chakoli",slug:"ali-nabipour-chakoli",fullName:"Ali Nabipour Chakoli",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/250668/images/system/250668.jpg",biography:"Academic Qualification:\r\n•\tPhD in Materials Physics and Chemistry, From: Sep. 2006, to: Sep. 2010, School of Materials Science and Engineering, Harbin Institute of Technology, Thesis: Structure and Shape Memory Effect of Functionalized MWCNTs/poly (L-lactide-co-ε-caprolactone) Nanocomposites. Supervisor: Prof. Wei Cai,\r\n•\tM.Sc in Applied Physics, From: 1996, to: 1998, Faculty of Physics & Nuclear Science, Amirkabir Uni. of Technology, Tehran, Iran, Thesis: Determination of Boron in Micro alloy Steels with solid state nuclear track detectors by neutron induced auto radiography, Supervisors: Dr. M. Hosseini Ashrafi and Dr. A. Hosseini.\r\n•\tB.Sc. in Applied Physics, From: 1991, to: 1996, Faculty of Physics & Nuclear Science, Amirkabir Uni. of Technology, Tehran, Iran, Thesis: Design of shielding for Am-Be neutron sources for In Vivo neutron activation analysis, Supervisor: Dr. M. Hosseini Ashrafi.\r\n\r\nResearch Experiences:\r\n1.\tNanomaterials, Carbon Nanotubes, Graphene: Synthesis, Functionalization and Characterization,\r\n2.\tMWCNTs/Polymer Composites: Fabrication and Characterization, \r\n3.\tShape Memory Polymers, Biodegradable Polymers, ORC, Collagen,\r\n4.\tMaterials Analysis and Characterizations: TEM, SEM, XPS, FT-IR, Raman, DSC, DMA, TGA, XRD, GPC, Fluoroscopy, \r\n5.\tInteraction of Radiation with Mater, Nuclear Safety and Security, NDT(RT),\r\n6.\tRadiation Detectors, Calibration (SSDL),\r\n7.\tCompleted IAEA e-learning Courses:\r\nNuclear Security (15 Modules),\r\nNuclear Safety:\r\nTSA 2: Regulatory Protection in Occupational Exposure,\r\nTips & Tricks: Radiation Protection in Radiography,\r\nSafety and Quality in Radiotherapy,\r\nCourse on Sealed Radioactive Sources,\r\nCourse on Fundamentals of Environmental Remediation,\r\nCourse on Planning for Environmental Remediation,\r\nKnowledge Management Orientation Course,\r\nFood Irradiation - Technology, Applications and Good Practices,\r\nEmployment:\r\nFrom 2010 to now: Academic staff, Nuclear Science and Technology Research Institute, Kargar Shomali, Tehran, Iran, P.O. Box: 14395-836.\r\nFrom 1997 to 2006: Expert of Materials Analysis and Characterization. Research Center of Agriculture and Medicine. Rajaeeshahr, Karaj, Iran, P. O. Box: 31585-498.",institutionString:"Atomic Energy Organization of Iran",institution:{name:"Atomic Energy Organization of Iran",country:{name:"Iran"}}},{id:"248279",title:"Dr.",name:"Monika",middleName:"Elzbieta",surname:"Machoy",slug:"monika-machoy",fullName:"Monika Machoy",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/248279/images/system/248279.jpeg",biography:"Monika Elżbieta Machoy, MD, graduated with distinction from the Faculty of Medicine and Dentistry at the Pomeranian Medical University in 2009, defended her PhD thesis with summa cum laude in 2016 and is currently employed as a researcher at the Department of Orthodontics of the Pomeranian Medical University. She expanded her professional knowledge during a one-year scholarship program at the Ernst Moritz Arndt University in Greifswald, Germany and during a three-year internship at the Technical University in Dresden, Germany. She has been a speaker at numerous orthodontic conferences, among others, American Association of Orthodontics, European Orthodontic Symposium and numerous conferences of the Polish Orthodontic Society. She conducts research focusing on the effect of orthodontic treatment on dental and periodontal tissues and the causes of pain in orthodontic patients.",institutionString:"Pomeranian Medical University",institution:{name:"Pomeranian Medical University",country:{name:"Poland"}}},{id:"252743",title:"Prof.",name:"Aswini",middleName:"Kumar",surname:"Kar",slug:"aswini-kar",fullName:"Aswini Kar",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/252743/images/10381_n.jpg",biography:"uploaded in cv",institutionString:null,institution:{name:"KIIT University",country:{name:"India"}}},{id:"204256",title:"Dr.",name:"Anil",middleName:"Kumar",surname:"Kumar Sahu",slug:"anil-kumar-sahu",fullName:"Anil Kumar Sahu",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/204256/images/14201_n.jpg",biography:"I have nearly 11 years of research and teaching experience. I have done my master degree from University Institute of Pharmacy, Pt. Ravi Shankar Shukla University, Raipur, Chhattisgarh India. I have published 16 review and research articles in international and national journals and published 4 chapters in IntechOpen, the world’s leading publisher of Open access books. I have presented many papers at national and international conferences. I have received research award from Indian Drug Manufacturers Association in year 2015. My research interest extends from novel lymphatic drug delivery systems, oral delivery system for herbal bioactive to formulation optimization.",institutionString:null,institution:{name:"Chhattisgarh Swami Vivekanand Technical University",country:{name:"India"}}},{id:"253468",title:"Dr.",name:"Mariusz",middleName:null,surname:"Marzec",slug:"mariusz-marzec",fullName:"Mariusz Marzec",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/253468/images/system/253468.png",biography:"An assistant professor at Department of Biomedical Computer Systems, at Institute of Computer Science, Silesian University in Katowice. Scientific interests: computer analysis and processing of images, biomedical images, databases and programming languages. He is an author and co-author of scientific publications covering analysis and processing of biomedical images and development of database systems.",institutionString:"University of Silesia",institution:null},{id:"212432",title:"Prof.",name:"Hadi",middleName:null,surname:"Mohammadi",slug:"hadi-mohammadi",fullName:"Hadi Mohammadi",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/212432/images/system/212432.jpeg",biography:"Dr. Hadi Mohammadi is a biomedical engineer with hands-on experience in the design and development of many engineering structures and medical devices through various projects that he has been involved in over the past twenty years. Dr. Mohammadi received his BSc. and MSc. degrees in Mechanical Engineering from Sharif University of Technology, Tehran, Iran, and his PhD. degree in Biomedical Engineering (biomaterials) from the University of Western Ontario. He was a postdoctoral trainee for almost four years at University of Calgary and Harvard Medical School. He is an industry innovator having created the technology to produce lifelike synthetic platforms that can be used for the simulation of almost all cardiovascular reconstructive surgeries. He’s been heavily involved in the design and development of cardiovascular devices and technology for the past 10 years. He is currently an Assistant Professor with the University of British Colombia, Canada.",institutionString:"University of British Columbia",institution:{name:"University of British Columbia",country:{name:"Canada"}}},{id:"254463",title:"Prof.",name:"Haisheng",middleName:null,surname:"Yang",slug:"haisheng-yang",fullName:"Haisheng Yang",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/254463/images/system/254463.jpeg",biography:"Haisheng Yang, Ph.D., Professor and Director of the Department of Biomedical Engineering, College of Life Science and Bioengineering, Beijing University of Technology. He received his Ph.D. degree in Mechanics/Biomechanics from Harbin Institute of Technology (jointly with University of California, Berkeley). Afterwards, he worked as a Postdoctoral Research Associate in the Purdue Musculoskeletal Biology and Mechanics Lab at the Department of Basic Medical Sciences, Purdue University, USA. He also conducted research in the Research Centre of Shriners Hospitals for Children-Canada at McGill University, Canada. Dr. Yang has over 10 years research experience in orthopaedic biomechanics and mechanobiology of bone adaptation and regeneration. He earned an award from Beijing Overseas Talents Aggregation program in 2017 and serves as Beijing Distinguished Professor.",institutionString:"Beijing University of Technology",institution:null},{id:"255757",title:"Dr.",name:"Igor",middleName:"Victorovich",surname:"Lakhno",slug:"igor-lakhno",fullName:"Igor Lakhno",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/255757/images/system/255757.jpg",biography:"Lakhno Igor Victorovich was born in 1971 in Kharkiv (Ukraine). \nMD – 1994, Kharkiv National Medical Univesity.\nOb&Gyn; – 1997, master courses in Kharkiv Medical Academy of Postgraduate Education.\nPhD – 1999, Kharkiv National Medical Univesity.\nDSc – 2019, PL Shupik National Academy of Postgraduate Education \nLakhno Igor has been graduated from an international training courses on reproductive medicine and family planning held in Debrecen University (Hungary) in 1997. Since 1998 Lakhno Igor has worked as an associate professor of the department of obstetrics and gynecology of VN Karazin National University and an associate professor of the perinatology, obstetrics and gynecology department of Kharkiv Medical Academy of Postgraduate Education. Since June 2019 he’s a professor of the department of obstetrics and gynecology of VN Karazin National University and a professor of the perinatology, obstetrics and gynecology department of Kharkiv Medical Academy of Postgraduate Education . He’s an author of about 200 printed works and there are 17 of them in Scopus or Web of Science databases. Lakhno Igor is a rewiever of Journal of Obstetrics and Gynaecology (Taylor and Francis), Informatics in Medicine Unlocked (Elsevier), The Journal of Obstetrics and Gynecology Research (Wiley), Endocrine, Metabolic & Immune Disorders-Drug Targets (Bentham Open), The Open Biomedical Engineering Journal (Bentham Open), etc. He’s defended a dissertation for DSc degree \\'Pre-eclampsia: prediction, prevention and treatment”. Lakhno Igor has participated as a speaker in several international conferences and congresses (International Conference on Biological Oscillations April 10th-14th 2016, Lancaster, UK, The 9th conference of the European Study Group on Cardiovascular Oscillations). His main scientific interests: obstetrics, women’s health, fetal medicine, cardiovascular medicine.",institutionString:"V.N. Karazin Kharkiv National University",institution:{name:"Kharkiv Medical Academy of Postgraduate Education",country:{name:"Ukraine"}}},{id:"89721",title:"Dr.",name:"Mehmet",middleName:"Cuneyt",surname:"Ozmen",slug:"mehmet-ozmen",fullName:"Mehmet Ozmen",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/89721/images/7289_n.jpg",biography:null,institutionString:null,institution:{name:"Gazi University",country:{name:"Turkey"}}},{id:"243698",title:"M.D.",name:"Xiaogang",middleName:null,surname:"Wang",slug:"xiaogang-wang",fullName:"Xiaogang Wang",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/243698/images/system/243698.png",biography:"Dr. Xiaogang Wang, a faculty member of Shanxi Eye Hospital specializing in the treatment of cataract and retinal disease and a tutor for postgraduate students of Shanxi Medical University, worked in the COOL Lab as an international visiting scholar under the supervision of Dr. David Huang and Yali Jia from October 2012 through November 2013. Dr. Wang earned an MD from Shanxi Medical University and a Ph.D. from Shanghai Jiao Tong University. Dr. Wang was awarded two research project grants focused on multimodal optical coherence tomography imaging and deep learning in cataract and retinal disease, from the National Natural Science Foundation of China. He has published around 30 peer-reviewed journal papers and four book chapters and co-edited one book.",institutionString:"Shanxi Eye Hospital",institution:{name:"Shanxi Eye Hospital",country:{name:"China"}}},{id:"242893",title:"Ph.D. Student",name:"Joaquim",middleName:null,surname:"De Moura",slug:"joaquim-de-moura",fullName:"Joaquim De Moura",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/242893/images/7133_n.jpg",biography:"Joaquim de Moura received his degree in Computer Engineering in 2014 from the University of A Coruña (Spain). In 2016, he received his M.Sc degree in Computer Engineering from the same university. He is currently pursuing his Ph.D degree in Computer Science in a collaborative project between ophthalmology centers in Galicia and the University of A Coruña. His research interests include computer vision, machine learning algorithms and analysis and medical imaging processing of various kinds.",institutionString:null,institution:{name:"University of A Coruña",country:{name:"Spain"}}},{id:"267434",title:"Dr.",name:"Rohit",middleName:null,surname:"Raja",slug:"rohit-raja",fullName:"Rohit Raja",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRZkkQAG/Profile_Picture_2022-05-09T12:55:18.jpg",biography:null,institutionString:null,institution:null},{id:"294334",title:"B.Sc.",name:"Marc",middleName:null,surname:"Bruggeman",slug:"marc-bruggeman",fullName:"Marc Bruggeman",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/294334/images/8242_n.jpg",biography:"Chemical engineer graduate, with a passion for material science and specific interest in polymers - their near infinite applications intrigue me. \n\nI plan to continue my scientific career in the field of polymeric biomaterials as I am fascinated by intelligent, bioactive and biomimetic materials for use in both consumer and medical applications.",institutionString:null,institution:null},{id:"244950",title:"Dr.",name:"Salvatore",middleName:null,surname:"Di Lauro",slug:"salvatore-di-lauro",fullName:"Salvatore Di Lauro",position:null,profilePictureURL:"https://intech-files.s3.amazonaws.com/0030O00002bSF1HQAW/ProfilePicture%202021-12-20%2014%3A54%3A14.482",biography:"Name:\n\tSALVATORE DI LAURO\nAddress:\n\tHospital Clínico Universitario Valladolid\nAvda Ramón y Cajal 3\n47005, Valladolid\nSpain\nPhone number: \nFax\nE-mail:\n\t+34 983420000 ext 292\n+34 983420084\nsadilauro@live.it\nDate and place of Birth:\nID Number\nMedical Licence \nLanguages\t09-05-1985. Villaricca (Italy)\n\nY1281863H\n474707061\nItalian (native language)\nSpanish (read, written, spoken)\nEnglish (read, written, spoken)\nPortuguese (read, spoken)\nFrench (read)\n\t\t\nCurrent position (title and company)\tDate (Year)\nVitreo-Retinal consultant in ophthalmology. Hospital Clinico Universitario Valladolid. Sacyl. National Health System.\nVitreo-Retinal consultant in ophthalmology. Instituto Oftalmologico Recoletas. Red Hospitalaria Recoletas. Private practise.\t2017-today\n\n2019-today\n\t\n\t\nEducation (High school, university and postgraduate training > 3 months)\tDate (Year)\nDegree in Medicine and Surgery. University of Neaples 'Federico II”\nResident in Opthalmology. Hospital Clinico Universitario Valladolid\nMaster in Vitreo-Retina. IOBA. University of Valladolid\nFellow of the European Board of Ophthalmology. Paris\nMaster in Research in Ophthalmology. University of Valladolid\t2003-2009\n2012-2016\n2016-2017\n2016\n2012-2013\n\t\nEmployments (company and positions)\tDate (Year)\nResident in Ophthalmology. Hospital Clinico Universitario Valladolid. Sacyl.\nFellow in Vitreo-Retina. IOBA. University of Valladolid\nVitreo-Retinal consultant in ophthalmology. Hospital Clinico Universitario Valladolid. Sacyl. National Health System.\nVitreo-Retinal consultant in ophthalmology. Instituto Oftalmologico Recoletas. Red Hospitalaria Recoletas. \n\t2012-2016\n2016-2017\n2017-today\n\n2019-Today\n\n\n\t\nClinical Research Experience (tasks and role)\tDate (Year)\nAssociated investigator\n\n' FIS PI20/00740: DESARROLLO DE UNA CALCULADORA DE RIESGO DE\nAPARICION DE RETINOPATIA DIABETICA BASADA EN TECNICAS DE IMAGEN MULTIMODAL EN PACIENTES DIABETICOS TIPO 1. Grant by: Ministerio de Ciencia e Innovacion \n\n' (BIO/VA23/14) Estudio clínico multicéntrico y prospectivo para validar dos\nbiomarcadores ubicados en los genes p53 y MDM2 en la predicción de los resultados funcionales de la cirugía del desprendimiento de retina regmatógeno. Grant by: Gerencia Regional de Salud de la Junta de Castilla y León.\n' Estudio multicéntrico, aleatorizado, con enmascaramiento doble, en 2 grupos\nparalelos y de 52 semanas de duración para comparar la eficacia, seguridad e inmunogenicidad de SOK583A1 respecto a Eylea® en pacientes con degeneración macular neovascular asociada a la edad' (CSOK583A12301; N.EUDRA: 2019-004838-41; FASE III). Grant by Hexal AG\n\n' Estudio de fase III, aleatorizado, doble ciego, con grupos paralelos, multicéntrico para comparar la eficacia y la seguridad de QL1205 frente a Lucentis® en pacientes con degeneración macular neovascular asociada a la edad. (EUDRACT: 2018-004486-13). Grant by Qilu Pharmaceutical Co\n\n' Estudio NEUTON: Ensayo clinico en fase IV para evaluar la eficacia de aflibercept en pacientes Naive con Edema MacUlar secundario a Oclusion de Vena CenTral de la Retina (OVCR) en regimen de tratamientO iNdividualizado Treat and Extend (TAE)”, (2014-000975-21). Grant by Fundacion Retinaplus\n\n' Evaluación de la seguridad y bioactividad de anillos de tensión capsular en conejo. Proyecto Procusens. Grant by AJL, S.A.\n\n'Estudio epidemiológico, prospectivo, multicéntrico y abierto\\npara valorar la frecuencia de la conjuntivitis adenovírica diagnosticada mediante el test AdenoPlus®\\nTest en pacientes enfermos de conjuntivitis aguda”\\n. National, multicenter study. Grant by: NICOX.\n\nEuropean multicentric trial: 'Evaluation of clinical outcomes following the use of Systane Hydration in patients with dry eye”. Study Phase 4. Grant by: Alcon Labs'\n\nVLPs Injection and Activation in a Rabbit Model of Uveal Melanoma. Grant by Aura Bioscience\n\nUpdating and characterization of a rabbit model of uveal melanoma. Grant by Aura Bioscience\n\nEnsayo clínico en fase IV para evaluar las variantes genéticas de la vía del VEGF como biomarcadores de eficacia del tratamiento con aflibercept en pacientes con degeneración macular asociada a la edad (DMAE) neovascular. Estudio BIOIMAGE. IMO-AFLI-2013-01\n\nEstudio In-Eye:Ensayo clínico en fase IV, abierto, aleatorizado, de 2 brazos,\nmulticçentrico y de 12 meses de duración, para evaluar la eficacia y seguridad de un régimen de PRN flexible individualizado de 'esperar y extender' versus un régimen PRN según criterios de estabilización mediante evaluaciones mensuales de inyecciones intravítreas de ranibizumab 0,5 mg en pacientes naive con neovascularización coriodea secunaria a la degeneración macular relacionada con la edad. CP: CRFB002AES03T\n\nTREND: Estudio Fase IIIb multicéntrico, randomizado, de 12 meses de\nseguimiento con evaluador de la agudeza visual enmascarado, para evaluar la eficacia y la seguridad de ranibizumab 0.5mg en un régimen de tratar y extender comparado con un régimen mensual, en pacientes con degeneración macular neovascular asociada a la edad. CP: CRFB002A2411 Código Eudra CT:\n2013-002626-23\n\n\n\nPublications\t\n\n2021\n\n\n\n\n2015\n\n\n\n\n2021\n\n\n\n\n\n2021\n\n\n\n\n2015\n\n\n\n\n2015\n\n\n2014\n\n\n\n\n2015-16\n\n\n\n2015\n\n\n2014\n\n\n2014\n\n\n\n\n2014\n\n\n\n\n\n\n\n2014\n\nJose Carlos Pastor; Jimena Rojas; Salvador Pastor-Idoate; Salvatore Di Lauro; Lucia Gonzalez-Buendia; Santiago Delgado-Tirado. Proliferative vitreoretinopathy: A new concept of disease pathogenesis and practical\nconsequences. Progress in Retinal and Eye Research. 51, pp. 125 - 155. 03/2016. DOI: 10.1016/j.preteyeres.2015.07.005\n\n\nLabrador-Velandia S; Alonso-Alonso ML; Di Lauro S; García-Gutierrez MT; Srivastava GK; Pastor JC; Fernandez-Bueno I. Mesenchymal stem cells provide paracrine neuroprotective resources that delay degeneration of co-cultured organotypic neuroretinal cultures.Experimental Eye Research. 185, 17/05/2019. DOI: 10.1016/j.exer.2019.05.011\n\nSalvatore Di Lauro; Maria Teresa Garcia Gutierrez; Ivan Fernandez Bueno. Quantification of pigment epithelium-derived factor (PEDF) in an ex vivo coculture of retinal pigment epithelium cells and neuroretina.\nJournal of Allbiosolution. 2019. ISSN 2605-3535\n\nSonia Labrador Velandia; Salvatore Di Lauro; Alonso-Alonso ML; Tabera Bartolomé S; Srivastava GK; Pastor JC; Fernandez-Bueno I. Biocompatibility of intravitreal injection of human mesenchymal stem cells in immunocompetent rabbits. Graefe's archive for clinical and experimental ophthalmology. 256 - 1, pp. 125 - 134. 01/2018. DOI: 10.1007/s00417-017-3842-3\n\n\nSalvatore Di Lauro, David Rodriguez-Crespo, Manuel J Gayoso, Maria T Garcia-Gutierrez, J Carlos Pastor, Girish K Srivastava, Ivan Fernandez-Bueno. A novel coculture model of porcine central neuroretina explants and retinal pigment epithelium cells. Molecular Vision. 2016 - 22, pp. 243 - 253. 01/2016.\n\nSalvatore Di Lauro. Classifications for Proliferative Vitreoretinopathy ({PVR}): An Analysis of Their Use in Publications over the Last 15 Years. Journal of Ophthalmology. 2016, pp. 1 - 6. 01/2016. DOI: 10.1155/2016/7807596\n\nSalvatore Di Lauro; Rosa Maria Coco; Rosa Maria Sanabria; Enrique Rodriguez de la Rua; Jose Carlos Pastor. Loss of Visual Acuity after Successful Surgery for Macula-On Rhegmatogenous Retinal Detachment in a Prospective Multicentre Study. Journal of Ophthalmology. 2015:821864, 2015. DOI: 10.1155/2015/821864\n\nIvan Fernandez-Bueno; Salvatore Di Lauro; Ivan Alvarez; Jose Carlos Lopez; Maria Teresa Garcia-Gutierrez; Itziar Fernandez; Eva Larra; Jose Carlos Pastor. Safety and Biocompatibility of a New High-Density Polyethylene-Based\nSpherical Integrated Porous Orbital Implant: An Experimental Study in Rabbits. Journal of Ophthalmology. 2015:904096, 2015. DOI: 10.1155/2015/904096\n\nPastor JC; Pastor-Idoate S; Rodríguez-Hernandez I; Rojas J; Fernandez I; Gonzalez-Buendia L; Di Lauro S; Gonzalez-Sarmiento R. Genetics of PVR and RD. Ophthalmologica. 232 - Suppl 1, pp. 28 - 29. 2014\n\nRodriguez-Crespo D; Di Lauro S; Singh AK; Garcia-Gutierrez MT; Garrosa M; Pastor JC; Fernandez-Bueno I; Srivastava GK. Triple-layered mixed co-culture model of RPE cells with neuroretina for evaluating the neuroprotective effects of adipose-MSCs. Cell Tissue Res. 358 - 3, pp. 705 - 716. 2014.\nDOI: 10.1007/s00441-014-1987-5\n\nCarlo De Werra; Salvatore Condurro; Salvatore Tramontano; Mario Perone; Ivana Donzelli; Salvatore Di Lauro; Massimo Di Giuseppe; Rosa Di Micco; Annalisa Pascariello; Antonio Pastore; Giorgio Diamantis; Giuseppe Galloro. Hydatid disease of the liver: thirty years of surgical experience.Chirurgia italiana. 59 - 5, pp. 611 - 636.\n(Italia): 2007. ISSN 0009-4773\n\nChapters in books\n\t\n' Salvador Pastor Idoate; Salvatore Di Lauro; Jose Carlos Pastor Jimeno. PVR: Pathogenesis, Histopathology and Classification. Proliferative Vitreoretinopathy with Small Gauge Vitrectomy. Springer, 2018. ISBN 978-3-319-78445-8\nDOI: 10.1007/978-3-319-78446-5_2. \n\n' Salvatore Di Lauro; Maria Isabel Lopez Galvez. Quistes vítreos en una mujer joven. Problemas diagnósticos en patología retinocoroidea. Sociedad Española de Retina-Vitreo. 2018.\n\n' Salvatore Di Lauro; Salvador Pastor Idoate; Jose Carlos Pastor Jimeno. iOCT in PVR management. OCT Applications in Opthalmology. pp. 1 - 8. INTECH, 2018. DOI: 10.5772/intechopen.78774.\n\n' Rosa Coco Martin; Salvatore Di Lauro; Salvador Pastor Idoate; Jose Carlos Pastor. amponadores, manipuladores y tinciones en la cirugía del traumatismo ocular.Trauma Ocular. Ponencia de la SEO 2018..\n\n' LOPEZ GALVEZ; DI LAURO; CRESPO. OCT angiografia y complicaciones retinianas de la diabetes. PONENCIA SEO 2021, CAPITULO 20. (España): 2021.\n\n' Múltiples desprendimientos neurosensoriales bilaterales en paciente joven. Enfermedades Degenerativas De Retina Y Coroides. SERV 04/2016. \n' González-Buendía L; Di Lauro S; Pastor-Idoate S; Pastor Jimeno JC. Vitreorretinopatía proliferante (VRP) e inflamación: LA INFLAMACIÓN in «INMUNOMODULADORES Y ANTIINFLAMATORIOS: MÁS ALLÁ DE LOS CORTICOIDES. 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