Lactic acid bacteria (LAB) species grouped according to their morphology and fermentative pathway, isolated worldwide from grapes, musts, and wines (adapted from [11, 12, 13, 14, 15, 16]).
\r\n\tAssisted Reproductive Technologies (ART) is a key technology for treating infertility, which occurs in 10-15% of the general population in reproductive age. This has been one of the most tumultuously developing interdisciplinary technologies in medicine in recent decades. Thanks to in vitro methods, more than 5 million children were born in the whole world. For 40 years, the success rates of this treatment have increased many times, respectively in the first years from less than 10% to more than 50% in present days (in some groups of patients). The reason for this rapid increase is the introduction of new drugs and stimulation protocols, improvement of embryo culture media, and the use of new types of laboratory equipment that improve the conditions for embryo development. Last but not least, the introduction of modern genetic methods, as well as new gamete and tissue freezing techniques, has improved the methods' diagnostic and therapeutic capabilities.
",isbn:"978-1-80356-720-4",printIsbn:"978-1-80356-719-8",pdfIsbn:"978-1-80356-721-1",doi:null,price:0,priceEur:0,priceUsd:0,slug:null,numberOfPages:0,isOpenForSubmission:!0,isSalesforceBook:!1,hash:"4a171468ca00ae2c47f7f5cd0f3b90a4",bookSignature:"Dr. Iavor K. Vladimirov",publishedDate:null,coverURL:"https://cdn.intechopen.com/books/images_new/11839.jpg",keywords:"Ovarian Reserve, Indication of ART Treatment, Stimulation Protocols, Monitoring of Ovarian Stimulation, Oocytes and Sperm Preparation, Manipulation Techniques, Surrogacy, Cross Border IVF Treatment, Preimplantation Genetic Testing, Endometrial Receptivity, Ethical aspects of ART, Ovarian Rejuvenation",numberOfDownloads:null,numberOfWosCitations:0,numberOfCrossrefCitations:null,numberOfDimensionsCitations:null,numberOfTotalCitations:null,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"March 24th 2022",dateEndSecondStepPublish:"June 7th 2022",dateEndThirdStepPublish:"August 6th 2022",dateEndFourthStepPublish:"October 25th 2022",dateEndFifthStepPublish:"December 24th 2022",remainingDaysToSecondStep:"20 days",secondStepPassed:!1,currentStepOfPublishingProcess:2,editedByType:null,kuFlag:!1,biosketch:'Dr. Vladimirov is the founder and medical director of the Sofia IVF Clinic. He is also one of the authors of the book "Theory about the Embryo Cryo-Treatment", the first theory that provides a scientific explanation for the high success rate in the use of frozen embryos.\r\nDr. Vladimirov introduces for the first time in Bulgaria the methods for Ovarian Reserve Assessment, In Vitro Maturation method,Determination of “implantation window” in the endometrium, using\r\nEndometrial Receptivity Analysis (ERA) test.',coeditorOneBiosketch:null,coeditorTwoBiosketch:null,coeditorThreeBiosketch:null,coeditorFourBiosketch:null,coeditorFiveBiosketch:null,editors:[{id:"253947",title:"Dr.",name:"Iavor K.",middleName:null,surname:"Vladimirov",slug:"iavor-k.-vladimirov",fullName:"Iavor K. Vladimirov",profilePictureURL:"https://mts.intechopen.com/storage/users/253947/images/system/253947.jpg",biography:"Dr. Iavor K. Vladimirov graduated in Medicine at the Sofia Medical University in 1990. In 1997 he became an Obstetrics and Gynaecology specialist. In 2003, he wrote a doctoral thesis on 'Diagnostic Testing to Assess the Ovarian Reserve of Infertile Women”, and obtained a Ph.D.\nDr. Vladimirov was, in the period between 2008 and 2010, a secretary of the Bulgarian Association of Sterility and Reproductive Health. \nHe hаѕ оvеr 25 уеаrѕ’ of ехреrіеnсе іn the аѕѕіѕtеd rерrоduсtіоn and іntrоduсеd the method of 'іn vіtrо mаturаtіоn” tо Вulgаrіа. Dr. Iavor Vladimirov іѕ thе fіrѕt Вulgаrіаn rерrоduсtіvе mеdісіnе ѕресіаlіѕt whо wоn оnе оf thе mоѕt рrеѕtіgіоuѕ аwаrd оf 'Тhе Society for Reproductive Endocrinology and Infertility” оf Тhе Аmеrісаn Ѕосіеtу fоr Rерrоduсtіvе Меdісіnе (АЅRМ) fоr уеаr 2016 and won the prestigious \\'Daniela Seizova - In the Name of Life\\' award in the category \\'Physician of 2016.\\' \nSince 2004 he is the Medical Director of the IVF unit in SBALAGRM – Sofia, Bulgaria.\nSince 2008 he is a Lector of the Faculty of Biology, Sofia University 'St. Kliment Ohridski”, Sofia, Bulgaria.",institutionString:"Sofia IVF clinic",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"1",totalChapterViews:"0",totalEditedBooks:"0",institution:null}],coeditorOne:null,coeditorTwo:null,coeditorThree:null,coeditorFour:null,coeditorFive:null,topics:[{id:"16",title:"Medicine",slug:"medicine"}],chapters:null,productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"},personalPublishingAssistant:{id:"453622",firstName:"Tea",lastName:"Jurcic",middleName:null,title:"Ms.",imageUrl:"//cdnintech.com/web/frontend/www/assets/author.svg",email:"tea@intechopen.com",biography:null}},relatedBooks:[{type:"book",id:"6550",title:"Cohort Studies in Health Sciences",subtitle:null,isOpenForSubmission:!1,hash:"01df5aba4fff1a84b37a2fdafa809660",slug:"cohort-studies-in-health-sciences",bookSignature:"R. 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Venkateswarlu",coverURL:"https://cdn.intechopen.com/books/images_new/371.jpg",editedByType:"Edited by",editors:[{id:"58592",title:"Dr.",name:"Arun",surname:"Shanker",slug:"arun-shanker",fullName:"Arun Shanker"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"72",title:"Ionic Liquids",subtitle:"Theory, Properties, New Approaches",isOpenForSubmission:!1,hash:"d94ffa3cfa10505e3b1d676d46fcd3f5",slug:"ionic-liquids-theory-properties-new-approaches",bookSignature:"Alexander Kokorin",coverURL:"https://cdn.intechopen.com/books/images_new/72.jpg",editedByType:"Edited by",editors:[{id:"19816",title:"Prof.",name:"Alexander",surname:"Kokorin",slug:"alexander-kokorin",fullName:"Alexander Kokorin"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"314",title:"Regenerative Medicine and Tissue Engineering",subtitle:"Cells and Biomaterials",isOpenForSubmission:!1,hash:"bb67e80e480c86bb8315458012d65686",slug:"regenerative-medicine-and-tissue-engineering-cells-and-biomaterials",bookSignature:"Daniel Eberli",coverURL:"https://cdn.intechopen.com/books/images_new/314.jpg",editedByType:"Edited by",editors:[{id:"6495",title:"Dr.",name:"Daniel",surname:"Eberli",slug:"daniel-eberli",fullName:"Daniel Eberli"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"57",title:"Physics and Applications of Graphene",subtitle:"Experiments",isOpenForSubmission:!1,hash:"0e6622a71cf4f02f45bfdd5691e1189a",slug:"physics-and-applications-of-graphene-experiments",bookSignature:"Sergey Mikhailov",coverURL:"https://cdn.intechopen.com/books/images_new/57.jpg",editedByType:"Edited by",editors:[{id:"16042",title:"Dr.",name:"Sergey",surname:"Mikhailov",slug:"sergey-mikhailov",fullName:"Sergey Mikhailov"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"1373",title:"Ionic Liquids",subtitle:"Applications and Perspectives",isOpenForSubmission:!1,hash:"5e9ae5ae9167cde4b344e499a792c41c",slug:"ionic-liquids-applications-and-perspectives",bookSignature:"Alexander Kokorin",coverURL:"https://cdn.intechopen.com/books/images_new/1373.jpg",editedByType:"Edited by",editors:[{id:"19816",title:"Prof.",name:"Alexander",surname:"Kokorin",slug:"alexander-kokorin",fullName:"Alexander Kokorin"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}}]},chapter:{item:{type:"chapter",id:"63846",title:"Lactic Acid Bacteria Contribution to Wine Quality and Safety",doi:"10.5772/intechopen.81168",slug:"lactic-acid-bacteria-contribution-to-wine-quality-and-safety",body:'\nThis review focuses on the current knowledge about the impact of lactic acid bacteria (LAB) in wine composition and flavor. In wine, LAB perform a second fermentation consisting of decarboxylating L-malic acid to L-lactic acid, designated by malolactic fermentation (MLF). This fermentation follows the alcoholic fermentation conducted by yeast (
Under favorable conditions, MLF occurs spontaneously after alcoholic fermentation by the growth of indigenous LAB population in wine. However, selected strains of LAB can be inoculated into wine to induce MLF. According to the types of wines produced, this biological deacidification may be considered beneficial or detrimental to wine quality. However, it should be highlighted that some LAB species in particular homofermentative pediococci and heterofermentative lactobacilli are responsible for wine spoilage [1].
\nLAB metabolic activity that may have a very significant impact on wine flavor includes the metabolism of citric acid and amino acids, the hydrolysis of grape glycosides, and the synthesis and hydrolysis of esters. Yet, other reactions can lead to the production of biogenic amines and ethyl carbamate by some LAB strains with negative consequences to wine safety.
\nAlthough it is not possible to have a clear definition of lactic acid bacteria (LAB), this group of bacteria is mainly characterized by the production of lactic acid as a major catabolic end product from glucose [2]. The other main characteristics of LAB are gram-positive cocci or bacilli, non-sporing, generally nonmotile, catalase negative, aerotolerant, acid tolerant, chemoorganotrophic, and strictly fermentative organisms. In some conditions, such as media containing hematin or related compounds, some strains may produce catalase or even cytochromes [3]. Though aerotolerant, they are a group of bacteria typical of non-aerobic habitats, very demanding from a nutritional point of view and tolerate very low pH values, with acidity tolerance being a variable trait among strains. LAB are present in very diverse environments (e.g., fermented foods and beverages, plants, fruits, soil, wastewater) and are also part of the microflora of the respiratory, intestinal, and genital tracts of man and animals [4, 5].
\nLactic acid bacteria (LAB) are naturally part of the microbiota of grapes, musts, and wines. In musts and wines, the LAB species that may be present and isolated are (i) heterofermentative cocci belonging to
Morphology | \nFermentation type | \nSpecies | \n
---|---|---|
Bacilli | \nFacultative heterofermentative | \n|
Heterofermentative | \n||
Homofermentative | \n||
Cocci | \nHomofermentative | \n|
Heterofermentative | \n
Either complexity or multiplicity of LAB metabolic activities in wine demonstrates that MLF is more than a simple decarboxylation of L-malic acid into L-lactic acid, and thus this very special and important fermentation may affect positively and/or negatively the quality of wine [17].
\nBesides the immediate effect of decrease in acidity by the transformation of a dicarboxylic acid (L-malic acid) into a monocarboxylic acid (L-lactic acid), MLF also improves sensorial characteristics and increases wine microbiological stability [18, 19]. Modifications in wine aroma induced by LAB are due to L-lactic acid, less aggressive to palate, and a huge number of other compounds such as diacetyl, acetoin, 2,3-butanediol, ethyl lactate and diethyl succinate esters, and some higher alcohols and aromatic aglycones that become free by the action of LAB β-glucosidases [20, 21, 22, 23]. Although produced in lower concentrations, sulfur compounds, particularly 3-methylsulfanyl-propionic acid with chocolate and toasted odors, may contribute to aromatic complexity of wines [24]. Also the activity of taninoacil hydrolase enzyme, commonly termed tannase, reducing wine astringency and turbidity may increase the quality and result in a better and pleasant sensorial perception for consumers [25].
\nAlthough not well understood at that time, the knowledge of the negative role of LAB on wine quality comes from the first studies of Pasteur at the beginning of the twentieth century. Some wine defects due to microorganism development were accurately described and LAB were shown to be responsible for wine “diseases” such as “tourne,” the degradation of tartaric acid; “bitterness,” the degradation of glycerol; and “ropiness,” the unacceptable increase in wine viscosity [26]. Although less frequent nowadays, due to better hygienic conditions in wineries and knowledge of microorganisms, these wine “diseases” together with others such as butter aroma due to excessive production of diacetyl, flocculent growth, mannitol taint, and the geranium odor, presented in Table 2, still may occur. Also, the formation of volatile phenols (4-ethylguaiacol and 4-ethylphenol) and mousy off-odor by acetamide production of tetrahydropyridines can be produced by some strains of LAB species responsible for malolactic fermentation (MLF). Other compounds, such as ethyl carbamate formed by the degradation of arginine and biogenic amines (histamine, tyramine, and putrescine) from the degradation of amino acids, contribute negatively to wine quality and may affect the consumer’s health [18, 29, 30].
\nDeterioration | \nCompounds | \nSensory descriptor | \nResponsible microorganisms | \nAroma threshold | \n
---|---|---|---|---|
Acrolein | \nBitterness | \nPediococcus | \n\n | |
Butter aroma due to excessive diacetyl production | \n2,3-Butanedione (diacetyl) | \nButtery, nutty, caramel | \n0, 1–2 mgl−1 | \n|
Flocculent growth | \n\n | \n | \n | |
“Mannitol taint” manitic fermentation reduction of fructose to mannitol | \nMannitol | \nViscous, sweet, irritating finish | \n\n | |
Formation of volatile phenols (4-ethylguaiacol and 4-ethylphenol) by degradation of phenolic acids mainly ferulic acid and | \n4-Ethylguaiacol and 4-ethylphenol | \n\n | Lactobacillus spp. | \n\n |
Formation of glyoxal and methylglyoxal | \n\n | \n | \n | |
Ropiness production of extracellular polysaccharides that increase the viscosity of wine | \nβ-D-glucan (exopolysaccharide) | \nRopy, oily, thick viscous, slimy, texture | \n\n | |
“Geranium odor” reduction of sorbic acid to 2,4-hexadienol which esterifies with ethanol to give 2-ethoxyhexa-3,5-diene, responsible for geranium odor | \n2-Ethoxy-3,5-hexadiene | \nCrushed geranium leaves | \n0.1 μgl−1, | \n|
Mousiness production of tetrahydropyridines | \n2-Acetyl-tetrahydropyridine (ACTPY), 2-ethyltetrahydropyridine (ETPY), 2-acetyl-1-pyrroline (ACPY) | \nCaged mouse | \nLactobacillus | \n4–5 μgl−1, 2–18 μgl−1; 7–8 μgl−1 | \n
Production of biogenic amines (histamine, tyramine, putrescine) by decarboxylation of amino acids | \nHistamine, tyramine, putrescine | \n\n | \n | |
Production of ethyl carbamate precursors | \nEthyl carbamate | \n\n | \n | |
“Lactic peak” lactic fermentation of sugars, production of D-lactic acid and excessive production of acetic acid involved in the fermentation | \nAcetic acid | \n\n | 0.2 gl−1 | \n|
“Tourne disease” tartaric acid degradation | \nAcetic acid | \n\n | \n |
The main effect of malolactic fermentation is the decarboxylation of L-malic acid into L-lactic acid, catalyzed by the malolactic enzyme. However, lactic acid bacteria produce several volatile compounds that can significantly influence wine aroma. Acetic acid and acetoinic compounds (C4 compounds) are the major products of citric acid metabolism by LAB. Acetoinic compounds comprise diacetyl, acetoin, and 2,3-butanediol. The biosynthesis of these compounds depends on citric acid metabolism (Figure 1).
\nCitric acid metabolism by
Diacetyl (2,3-butanedione), one of the most important flavor compounds produced by LAB, imparts a distinct buttery or butterscotch aroma to wine. Diacetyl is formed as an intermediate metabolite of the reductive decarboxylation of pyruvate to 2,3-butanediol, associated with citrate metabolism by LAB. The precursor of diacetyl in this pathway is α-acetolactate, which is also an intermediate in the biosynthesis of the amino acids valine and leucine in prototrophic LAB. Pyruvic acid results from the metabolism of sugars and citric acid. To be capable to utilize citrate, LAB must possess the genes encoding permeases for citrate transport and citrate lyase for citrate metabolism [31].
\nYeasts are also able to synthesize diacetyl in the course of alcoholic fermentation. However, most of this diacetyl is reduced by yeasts to acetoin and 2,3-butanediol, and only low concentrations of diacetyl remain at the completion of fermentation. Diacetyl reduction is further encouraged by the presence of yeasts or LAB after the conclusion of malolactic fermentation [32].
\nSalo [33] determined a sensory odor threshold level of 0.0025 mg/L for diacetyl in 9.4% (w/w) ethanolic solution. Yet, Guth [34] calculated 0.1 mg/L for diacetyl odor threshold in water/ethanol (90 + 10, w/w). Moreover, Martineau et al. [35] showed that the diacetyl flavor threshold depends on the wine type. They found that the flavor detection threshold was 0.2 mg/L in a lightly aromatic Chardonnay wine, 0.9 mg/L in a low tannic aromatic Pinot noir wine, and 2.7 mg/L in a full-flavored, full-bodied Cabernet Sauvignon wine. These wines were made without oak contact.
\nReports of diacetyl concentration in wine vary from 0.2 to 4.1 mg/L [36]. The final concentration of diacetyl in wine depends on the concentration of sulfur dioxide. Sulfur dioxide combines reversibly with diacetyl in wine, suppressing the buttery note of wine flavor [37].
\nWine esters are important contributors to wine aroma. They comprise ethyl esters of organic acids (e.g., ethyl lactate), fatty acids (e.g., ethyl hexanoate, ethyl octanoate, ethyl decanoate), and acetates of higher alcohols (e.g., ethyl acetate, isoamyl acetate). These compounds are not only produced by yeasts during alcoholic fermentation and LAB during MLF but can also be formed by slow chemical esterification between alcohol and acids during wine aging [38]. LAB of the genera
Most volatile compounds that make the varietal aroma of wines are present in grapes in the form of glycoconjugated nonvolatile odorless molecules. These glycosides are β-D-glucose and diglycoside conjugates, with the latter consisting of glucose and a second sugar unit of α-L-arabinofuranose, α-L-rhamnopyranose, β-D-xylopyranose, or β-D-apiofuranose [42]. The aglycon moiety of these compounds belongs to different classes of volatiles, including monoterpenes and C13-norisoprenoids. The glycoconjugates can be slowly transformed into free volatile aroma compounds through acidic hydrolysis during wine aging. Yet, a faster enzymatic hydrolysis of these glycosides by wine microorganisms can also occur. The hydrolysis of the disaccharide glucosides requires the action of two enzymes in sequence: first the disaccharide (1 → 6) linkage is cleaved by the appropriate
Yeasts, mainly non-
The β-glucosidase activity of different strains of
Glycosidic activity is widespread in
LAB isolated from wine (including strains of
When sorbic acid ((E,E)-2,4-hexadienoic acid) as potassium sorbate is added as an yeast inhibitor to wines containing residual sugar, LAB can degrade this compound in 2-ethoxyhexa-3,5-diene (2-ethoxy-3,5-hexadiene). 2-Ethoxyhexa-3,5-diene has an offensive crushed geranium leaves odor with a detection threshold of less than 1 ng/L [49, 50]. Sorbic acid inhibits yeast growth, but it does not inhibit LAB growth at the levels allowed in wines for this compound, demonstrating the need for maintaining adequate levels of sulfur dioxide (an effective inhibitor of LAB) in such wines. When used together, sorbate and sulfur dioxide can prevent secondary fermentations and control the growth of LAB in sweet table wines, with a pH of 3.3–3.9, at levels as low as 80 mg/L sorbate and 30 mg/L sulfur dioxide [51].
\nVarious LAB isolated from wine showed the ability to synthesize 4-vinylphenol, by decarboxylation of
LAB use amino acids both as a strategy of survival particularly in nutrient limiting media and evidently in response to acid stress and as a source of energy. However, this may have implications for the quality and food safety of fermented products [53, 54].
\nThe metabolism of amino acids such as arginine and histidine does not affect taste but creates a problem at consumer’s health level by increasing the concentrations of biogenic amine and ethyl carbamate precursors in the wine, which are toxic compounds, thus contributing negatively to wine safety [55].
\nArginine is one of the amino acids present in higher concentrations in grape musts and wines. LAB may use this amino acid by arginine deaminase pathway. This pathway involves three enzymes: arginine deiminase (ADI, EC 3.5.3.6), ornithine transcarbamylase (OTC, EC 2.1.3.3), and carbamate kinase (CK; EC 2.7.2.2) [56]. The presence of the three enzymes of the ADI pathway appears to occur in most heterofermentative lactobacilli, leuconostocs, and oenococci, although they have already been detected in homofermentative species of LAB isolated from wine. However, the arginine pathway presence in all species seems to be a strain-dependent phenotype [57]. By this pathway, 1 mole of L-arginine is converted into 1 mole of ornithine and 1 mole of carbon dioxide and 2 moles of NH3. The intermediate products of this pathway, citrulline and carbamoyl phosphate, are precursors of the ethyl carbamate, a potentially carcinogenic compound. This compound is formed from a spontaneous chemical reaction involving ethanol and precursors including urea, citrulline, carbamoyl phosphate, N-carbamyl, α- and β-amino acids, and allantoin [58]. According to Ough et al. and Kodama et al. [59, 60], the ethanolysis reaction of citrulline and urea for ethyl carbamate formation may occur at normal or elevated storage temperatures. Even though ethyl carbamate is produced in small quantities, its concentration in wine is subjected to international regulation and therefore must be carefully controlled. Maximum level in the European Union and Canada for table wines is 30 μg/L (100 μg/L for fortified wines in Canada), while in the USA the values are more restrictive, being 15 μg/L for table wines and 60 μg/L for dessert wines [55, 60, 61].
\nSome controversial information about the contribution of LAB for ethyl carbamate production is found in the scientific literature [8]. Tegmo-Larsson et al. [62] reported that malolactic fermentation did not affect the concentrations of ethyl carbamate in wine. However, more recent information suggests that some lactic acid bacteria, specifically
Biogenic amines are low molecular weight organic bases, which can be formed and degraded during the normal metabolic activity of animals, plants, and microorganisms [29]. In the human body, these substances may play an important metabolic role, related to growth (polyamines) or to functions of the nervous and circulatory systems (histamine and tyramine). But when ingested in excess, they may be the cause of hypotension, hypertension, heart palpitations (vasoactive amines), headaches (psychoactive amines), and various allergic reactions [30, 66]. Biogenic amines are fundamentally formed from the decarboxylation of the precursor amino acids by the action of substrate specific enzymes [6, 67, 68]. Thus, the amines histamine, tyramine, tryptamine, serotonin, 2-phenylethylamine, agmatine, and cadaverine are formed from the amino acids histidine, tyrosine, tryptophan, hydroxytryptophan, phenylalanine, arginine, and lysine, respectively [69, 70, 71]. Putrescine can be formed from ornithine or agmatine, and spermidine and spermine are formed from putrescine by the binding of aminopropyl groups catalyzed by spermidine synthase and spermine synthase [72]. During the fermentative processes of many raw materials (milk, meat, vegetables, barley, and grapes) to obtain food and beverages, such as cheese, sausages, fermented vegetables, beer, and wine, the formation of biogenic amines by LAB may occur. Many bacteria present decarboxylase activities, which favor their growth and survival in acidic environments, by the increase of pH, as previously mentioned. In wine, several amino acids can be decarboxylated, and consequently, biogenic amines can be found, predominating histamine, tyramine, putrescine, isopentylamine, cadaverine, and α-phenylethylamine [29, 30, 73, 74, 75, 76, 77, 78, 79, 80, 81]. However, their content in wine is much lower than that found in other foods [82], although ethanol may potentiate the toxic effect of histamine by inhibiting amino oxidases. Like ethyl carbamate, there are recommendations for the maximum histamine levels allowed in wine. EU countries and Canada recommend histamine levels not exceeding 10 mg/L, except Germany where the limit is 2 mg/L. Some biogenic amines, for example, putrescine and cadaverine, when in high concentrations, besides their toxicity, can confer sensory detectable unpleasant alterations, such as a fruit and rotten flesh odor, respectively. In wine, although biogenic amines may have other sources such as grapes, the metabolic activity of
So, the existing content of biogenic amines in wine will depend on the presence of precursor amino acids, LAB strains with decarboxylase activity, and environmental factors that affect the growth of these strains as well as some oenological practices [30, 88, 89]. In general, low pH and high concentrations of SO2 and ethanol limit the growth of these strains and consequently the production of biogenic amines. On the other hand, factors favoring microbial growth such as high temperatures, availability of nutrients in must and wine (sugars, amino acids, organic acids), and inappropriate hygienic practices increase the probability of high amine concentrations [29]. As referred for the formation of ethyl carbamate, wines stored in prolonged contact with lees show higher levels of biogenic amines, attributed to viable but non cultivable LAB cells [30]. Generally, higher biogenic amine contents are found in red wines comparing to rosé, white, and fortified wines [86, 90, 91].
\nThe contribution of LAB to wine flavor and composition has been described in this review. The difficulties in controlling and anticipating the effects of malolactic fermentation (MLF) on wine quality, given ample species and strain-dependent behavior, remark the importance of strain selection to explore the genomic diversity of LAB.
\nSelection of starter cultures for MLF should target good adaptation to the harsh wine conditions and potential for the production of flavor compounds, emphasizing in particular glycosidase and esterase activities. Also, the absence of arginine deaminase pathway and amino acid decarboxylases and ability to detoxify mycotoxins such as ochratoxin [92] and biogenic amine degradation [93, 94] should be considered as criteria for LAB strain selection for using as starter cultures.
\nThe authors gratefully acknowledge support from project INNOVINE & WINE—Innovation Platform of Vine and Wine—NORTE-01-0145-FEDER-000038.
\nWith the development and complication of the structure of the energy facilities, the main problem of electric power industry is to ensure stable operation of power plants and transport of energy to the consumers. In this regard, the role of protection from external influences, especially from lightning, is growing steadily. The numerous experts’ statements noted that the ground wires of high voltage line (HVL) supports of stations and cable ways on power plants do not provide the required reliability of the electric grids for the highest voltage classes. Statistics of accidents and breakdowns in the operation of equipment energy indicates a high rate of lightning outages. They ranged from 20 to 50% of the total number of disconnections. The lightning storm outages have negative effect on the station operation, lines, and substation equipment, reducing the switch operational life and causing overvoltage switching on the equipment. Lightning also causes interference in chains of secondary commutation and devices of microprocessor technology, by means of induced electromagnetic fields. Because a lightning refers under the category of natural phenomena which is difficult to study, there is a great interest in full scale modeling effects of lightning. Full simulation of the effects of lightning in some cases is the only source of reliable information on the grounds that it affects on multiple related objects HVL and power plants, as well as to cause nonlinear processes in soils and ground wires when spreading the lightning currents. Understanding of the mechanisms and effects of lightning basic parameters for modeling were taken from work [1].
In the first part of the chapter presents the results of a full scale simulation of lightning currents, affecting directly the grounding devices designed to ensure the safe operation of power facilities. For these purposes have been designed, manufactured, and tested the mobile test complex (MTC EMG). A feature of this complex was the use of a helical explosive magnetic generator (EMG) as a pulse current source. EMG can convert the energy of the explosive in the electromagnetic energy that issues the current pulse in the load. Physical principles of work of explosive magnetic generators are reflected in a lot of classical works. For example, [2] gives the base of physical effects and the methods of generation high level electromagnetic fields. In [3], much attention is paid to EMG with a metal armature, the processes of energy conversion of detonation products into electrical energy pulses, and their efficiency. In [4], various schemes used to connect EMG to loads are discussed. References [5, 6] present the experimental and theoretical work of the Sarov’s nuclear center in the direction of lightning simulation using EMG on large grounded objects. EMG using in the experiments to examine spark soil processes [7], allowed us to represent the level of energy and pulse values of generated currents. The ways of transition from theory to practical schemes that use EMG in mobile testing facility were reported by authors in [8] firstly. It demands the reduction of explosive weight that allows realization of EMG, as a consumable item of MTC EMG. The theory of energy transfer from the EMG to high impedance load [9] was used for efficiently transfer energy from the EMG with using transformer circuits. Mathematical modeling of the dynamics of the EMG in a circuit with concentrated parameters allowed choosing the law of inductance output in the operation of EMG to provide a given front of the current pulse in the load without the use of switches. The theoretical background and mathematical modeling of such scheme are described in [10, 11]. In [10], the good agreement of the results of mathematical modeling and experimental measurements is illustrated. This chapter provides more complete results of field tests of MTC EMG. The active inductive load of two types is considered. In the first case, this is the ground loop. In the second case, it is a special model load. The ground loop forms an active load resistance of 2–4 Ω. In the case of the model load, the resistivity is increased to 10 Ω. The results of field tests showed stable reproduction of pulse values of currents at reliability, safety, and mobility of the complex.
The second part of this chapter presents the results of modeling the effects of high power electromagnetic impulse (EMI), disturbing the stability of energy systems. Such impact is not only on the result of a natural disaster—a thunderstorm, but also occurs at the use of electromagnetic pulse weapons and nuclear explosions. The pulse effect of lightning is accompanied by a change in the induction of the magnetic field in time. In this work, the near field of EMI impact was experimentally modeled by means of a source made on the basis of an explosive magnetic generator. The objects of influence were electronic measuring devices used in the experiment and made in a protected design. The front of the pulse current of EMI source was sharpened by a fast key on the basis of electro-explosive conductors (EEС) placed in the load circuit. The regime of rapid explosion was provided by the intense short-term action of the pulse current on the EEC. Reports collected in the chapter [12] are the basis for understanding the physics of the phenomenon of explosion conductors. The theory of explosion of EEC together with similarity criterion reflecting the relationship of mode of energy release in a conductor during the explosion with its physical properties was used in the selection of parameters of EEC [13, 14, 15, 16, 17, 18, 19, 20]. As shown in [14, 15], input in EEC energy sufficient for sublimation of conductors was the main requirement. Criterion for the optimal explosion of conductors in the case of capacitive storage as a pulsed energy source was considered in [17]. We used this approach when we started to simulate the explosion of conductors with a pulse source according to the Marx-Arkadyev scheme. The results were reported at the previous conferences on lightning protection [20]. The use of EMG is reported in this chapter. Two experiments were conducted for the same sources. The first experiment was prepared on the basis of preliminary calculations and was a trial one for the second. The measuring and recording equipment includes: control and measuring equipment, including Rogowski coil, voltage divider, oscilloscopes, and high speed camera HX3 of Japanese production for registration of the rapid process of explosion of the EEC. Evidence of a powerful EMI impact on electronic equipment was the fact that none of the devices could not record the data due to the interference and failure except for a specially designed device. This device was designed for other tasks as a recorder of lightning current. In spite of the use of standard means of protection against electromagnetic interference (metal housing grounded at a common point, isolated power supplies, and shielding), the level of exposure was high.
The results of both experimental and theoretical studies of the models presented were presented at conferences on Interaction of Intense Energy Fluxes with Matter, Elbrus, Kabardino-Balkaria, Russia “Elbrus” and Lightning Protection, St. Petersburg, Russia from 2012 to 2018. One of the presentations of the authors can be found on the conference website [20].
The third part of this chapter is devoted to the importance of full scale lightning simulation for lightning protection systems of power plants in terms of technical and economic indicators. The effectiveness assessments are based on the theory set out in the report at the lightning protection conference held in St. Petersburg in April 2018, a reference to the report is in [21].
During the development of the MTC EMG, several technical solutions were tested. The necessary requirements for the complex are: stable reproduction of pulse values of currents, while in operation for high voltage source, mobility, reliability, and safety. In the circuitry of MTC EMG explosive current breaker in the EMG circuit and closer in the load circuit were excluded, as they have essentially nonlinear characteristics and do not allow reproducing pulses of similar energy. The absence of untriggered discharger in the load circuit relieved the voltage jump when the transformer was in no load operation. In the new circuit, the load is always connected to the secondary winding of the pulse transformer (PT), and the EMG is directly connected to the primary winding of the PT. It gives possible to control the voltage on the load, setting the desired mode of operation of the EMG.
The formation of the front of the current pulse was provided by the design of the EMG. Special attention was paid to the geometry of the final section of the generator. The use of conical geometry in comparison with cylindrical geometry leads to an increase in the liner sliding speed. It is important that in these generators, the increase in the speed of the liner sliding along the spiral is achieved not by using a more powerful explosive, but due to the optimal angle of the cone spiral. For matching the EMG and the load, losses in the primary circuit of the transformer and the inductive-ohmic nature of the load were taken into account. Estimates made with the help of an equivalent circuit of substitution [10, 11] made it possible to obtain a condition for minimizing losses in the primary circuit, depending on the parameters of the circuit. At the same time, the efficiency of the energy transfer of the EMG to the inductive-ohmic load began to depend not only on the coupling coefficient of the pulse transformer windings and the ratio of the load inductance and the inductance of the secondary transformer winding, but also on the ratio of the active resistances of the primary and second transformer windings. In such a scheme, shown in Figure 1, the energy is transferred into the load both during the operation of the EMG and after completion, and thus, the pulse transformer is actually the storage of electromagnetic energy. In this case, the dissipation of a part of the energy in the primary circuit after the termination of the work of the EMG occurs. Estimates showed that when the condition for minimizing losses in the primary circuit is fulfilled, the efficiency of the transfer of energy into the load may exceed 50%.
Schematic diagram of the generator of lightning currents with the connected load.
MTC EMG is located on two KAMAZ vehicles. The lightning current generator, where EMG is the main element and refers to the spiral type, is mounted on the first four-axle base vehicle. The control panel of the test complex is located on the second three-axle base vehicle. The main elements of the lightning current generator are shown in Figure 2.
The main elements of the lightning current generator: 1—EMG; 2—explosion protection chamber; 3—pulse transformer; 4—container body; 5—bushing insulator.
The most important elements of the lightning current generator are the EMG and the pulse transformer (PT). EMG is placed in an explosive protection chamber, which excludes the destruction of the equipment of the complex during the operation. The explosion chamber is capable of withstanding explosions, which correspond to 5 kg in TNT equivalent. Thus, the EMG is the only consumable element of the MTC EMG. To match the EMG with the load, a pulse transformer with a low inductance primary winding is used. The transformer provides a maximum voltage on the primary/secondary windings of 100/1500 kV and a maximum current in the primary/secondary windings of 5000/100 kA. The transformer ratio is 55. The winding coupling coefficient is not less than 0.95. The design takes into account the requirements for limiting weight and increase in strength. The total weight of the equipment does not exceed 80% of the carrying capacity of the vehicle to ensure cross-country capability. The primary and secondary windings are made on one cylinder. High voltage output is carried out through a bushing insulator with a height of about 3 m. The commands between the control panel and the lightning current generator are transferred via fiber-optic communication lines. The measuring complex consists of self-contained recorder oscillograph. Recorders have autonomous power supply and are in a shielded enclosure. The recorder can be placed under any potential.
Most technical solutions are patented. In this scheme, a capacitive storage of electric energy is used as an initial energy source for creating an initial magnetic field in the EMG with an energy of up to 100 kJ. The initialization system is designed to initiate an explosive charge in the generator. The system does not contain electric detonators, which significantly increases the safety of work with explosives.
Field tests for an active load of up to 4 Ω were carried out in the east of the Moscow region. The test stand was located on the territory of approximately 70 m × 70 m. The photo of the placement of the MIK VMG and the experimental diagram are shown in Figure 3. The load was the ground between two ground loops. Ground loops were made of an aluminum wire with a diameter of 10–12 mm, located at a depth of 0.5 m. The external ground loop was 55 m × 55 m square. The internal ground loop in the first experiment was 15 m × 15 m, which corresponded to a load resistance of 2 Ω, in the second—4 m × 4 m (load resistance—4 Ω). The current was transmitted from the MTC EMG insulator mast to the pulse input rod into the internal circuit via the current transmission line. The total inductance, taking into account the air transmission line, did not exceed 80 μH.
Photo of field tests (a) and experimental diagram (b). 1—lightning current generator; 2—control machine; 3—impulse current input rod; 4—internal input circuit (dashed for the 2nd experiment); 5—ground loop; 6–9—voltage dividers; 10 and 11—Rogowski coils.
During the tests, the following parameters were recorded: initial current (powering current) of the EMG, current of the EMG (current of the primary winding of the PT), current in the load (current in the secondary winding of the PT), voltage at the MTC EMG output, that is, on inductive-active load, and voltage on the active load.
Rogowski coils were designed to record the derivative of current. They were used without integrators. The current was calculated by software integration of the data, taking into account the sensitivity of each coil. Voltage dividers were used to measure the voltage. The first voltage divider was connected to the high voltage output of the MTC EMG. The second voltage divider was connected to the load. To improve the transient characteristics, low inductance carbon resistors with a total resistance of 20 kΩ were used, which were located in a fiberglass pipe filled with transformer oil. To reduce the effect of parasitic capacitance of the resistor to the ground, when measuring, a pulsed current transformer on a ferrite core with a transformation ratio of 1:55 was used in a divider. This gave a galvanic isolation between the secondary circuit with the oscillograph and the primary circuit with the measured signal. Output voltage ratio was 1:220,000 (~1 MV:5 V).
Measurement of the resistance of the load (ground) was carried out before the beginning of the experiments. It is assumed that the potential of the outer loop due to its low resistance is zero. This assumption is satisfied (with an accuracy of 0.5%) for the experiment with an internal contour of 4 m × 4 m (experiment no. 2) and about 7% for an internal contour of 15 m × 15 m (experiment no. 1). The main results of the two tests (experiments) are presented in the graphs of Figures 4 and 5 and in Table 1.
The measured values of the currents in the load for the 1st (dotted line) and 2nd experiments.
The measured values of the voltage on the load for the 1st (pale color) and the 2nd experiments.
Parameters/experiment no. | No. 1 | No. 2 |
---|---|---|
Initial resistance between current input circuits (active load), Ω | 2 | 4 |
Load inductance, μH | 75 | 86 |
Initial energy of EMG, kJ | 23 | 43 |
Maximum amplitude of the current in the load, kA | 50 | 63 |
Energy generated by EMG, kJ | 900 | 1500 |
The maximum amplitude of the output voltage of MTC EMG, kV | 220 | 450 |
Maximum amplitude of the active load voltage, kV | 100 | 250 |
Rise time of the current pulse, μs | 30 | 25 |
Half-amplitude duration of the current pulse, μs | 120 | 80 |
Energy dissipated in the active load, kJ | 500 | 800 |
The increase in energy (energy in the active load with respect to the initial energy of the EMG) | 21 | 20 |
Parameters registered in the experiments.
Tests of the MTC EMG on the ground loop in the configuration, when the current flowed in the ground between the outer and inner contours, showed a linear increase in the voltage on the load with a decrease in the dimensions of the inner contour. A further increase in the load resistance in the tests became possible when the experimental scheme was changed and the use of model resistance.
MTC EMG field testing on the terminal parameter of the load specified in the development was carried out on a model load with an active resistance of 10 Ω and an inductance of about 150 μH. The tests were carried out on the territory of JIHT RAS, Shatura, Moscow region. Scheme and photo of the experiment are presented in Figure 6.
Scheme (a) and the photo (b) of the experiment for model load. SIE—source of initial energy with a capacitive storage; PT—pulse transformer; RL—resistive (active) load; LL—inductive load; RC 1… RC 3—Rogowski coils; HVD 1, HVD2—high voltage resistive dividers (1000 kV).
Figure 6a shows a diagram of the connection of the model load to the lightning current generator via a pulse transformer, as well as the location of the Rogowski coils for measuring currents and voltage dividers for measuring the voltage in the circuit. A model load has been developed and manufactured specifically for testing. The active load was modeled by a 10-Ω noninduction resistance.
In the foreground of Figure 6b, there is a lightning current generator on the first vehicle, on the left—an active load (a noninduction resistor) and two voltage dividers: at the MTC EMG output and on the load. In the background, there is the second vehicle with the control panel.
Resistance was installed vertically. The inductive load was modeled by wires connecting the resistive load. To reduce the size, the high voltage connection of the wire for the active load was made in the form of a spiral.
The main results of tests for the model load are presented in Table 2 and in the graphs of Figures 7–9. In these experiments, the signals were recorded on single-channel autonomous oscilloscopes; recording was performed at a given level of the input signal, which explains the time shift of the graphs relative to zero.
Parameter | Value |
---|---|
Active load resistance, Ω | 10 |
Load inductance, μH | 150 |
Voltage of the source of initial energy, kV | 62 |
Amplitude value of the powering current, kA | 115 |
The initial energy of the EMG, kJ | 73 |
The maximum derivative of the EMG current, kA/μs | 310 |
The maximum amplitude of the EMG current, kA | 6010 |
Energy generated by EMG, kJ | 900 |
The maximum amplitude of the voltage at the MTC EMG output, kV | 810 |
Maximum amplitude of the voltage on the active load, kV | 495 |
Maximum amplitude of the current in the load, kA | 44 |
The energy produced by the MTC EMG, kJ | 1640 |
Energy dissipated in the active load, kJ | 550 |
The increase in energy (energy in the active load with respect to the initial energy of the EMG) | 7 |
Rise time of the current pulse, μs | 37 |
Half-amplitude duration of the current pulse, μs | 50 |
Basic parameters in the tests for model load.
Voltage at the MTC EMG output from the voltage divider no. 1 and on the active load from the voltage divider no. 2.
Load current.
Schematic diagram of an experimental model with a power source based on the EMG. СOL and LOL—are the capacitance and inductance of overhead line; RC1—Rogowsi coil in the EMG circuit; RC2—Rogowsi coil in the load circuit; CT—the current transformer as part of voltage divider.
As can be seen from Tables 1 and 2, the increase in the active load resistance leads to a decrease in the energy transfer efficiency of the load from 55 to 33%, which corresponds to the theory [10, 11]. Thus, the use of MIC HMG for high resistance loads is less effective.
The pulse effect of lightning is accompanied by a change in the induction of the magnetic field in electrically conductive objects in time. The induction effect of the lightning channel is significant at distances commensurate with the length of the lightning channel. Induced voltages arise as a result of such influence on various technical means, for example, overhead transmission lines, grounding devices, etc. Induced voltage leads to the appearance of parasitic currents in the secondary circuits and external power cables due to the conductive coupling. The experimental model based on the EMG is a laboratory setup producing EMI impact on a limited space of 2 m × 2 m × 4 m. The schematic diagram of the electrical control and measuring equipment is shown in Figure 9.
The source of the pulse current is a helical-type explosive generator—EMG also. Output of current to the load is carried out through a pulse transformer (PT) and overhead transmission line (OL). The direct and reverse branches of the overhead lines are located on the insulator supports and are common for all experiments. The electrical explosion conductors (EEC) are at the end of the overhead line. General view of an experimental model is represented in Figure 10, and the view of EMG assembly is represented in Figure 11.
General view of an experimental model with EMG.
EMG assembly, before installation in the explosion protection chamber.
EEC in the EMI models is the key element. The regime of fast explosion is provided by the intense short-term action of the pulse current generated by the source. The rapid increase in resistance in exploding conductors leads to arising of a strong electric field. Together with the emerging magnetic field, an electromagnetic disturbance is formed by the EMI effect. The EEC in our models was made from the copper. Copper has a relatively low boiling point and low heat of vaporization. The current reaches the maximum by the end of evaporation if sufficient energy is supplied to the conductor [11]. The selection of EEC parameters, as the number, cross section, and length of the EEC, was carried out taking into account the similarity criteria reflecting the relationship of the character of the energy release in the explosion in the conductor with its physical properties. At the initial stage, the estimates were performed according to the criterion for capacitive energy sources set out in [16], but due to the difference in the pulse current fronts, they were overestimated in terms of the energy required for the explosion of conductors. The main criterion is the ratio of the transmitted EEC energy to the mass of the explosive conductor, which characterizes the initial stage of the explosion. From the balance of the inputted energy and the minimum specific energy required for the explosion, it is possible to estimate the limit mass of the conductors m = (W0/
We use the initial energy source for powering the EMG, it was about 15 kJ (a capacitor bank of 90 μF was charged to 18 kV). An overhead line inductance was 5.5 μH.
In the first experiment, 25 conductors of 120 μm diameter were taken. The full cross-sectional area of the conductors is 28⋅10−8 m2. The total mass of all conductors amounted to about 2.5 g. The number of conductors was determined by the requirement to obtain an explosion of conductors at the stage of current growth in the load. The specific energy obtained by the conductors is 12.5/2.4–5.2 kJ/g; it is roughly equal to the energy of the sublimation of copper. The experiment showed, see Figure 12, that the current was cut off (and the voltage increased abruptly) at the initial phase of the current rise. The current pause mode does not occur, and the further increase in the current is associated with the transition of the EEС into an electrically conductive plasma state. To achieve higher voltage amplitudes, we need to input more energy. This meant that we need to climb higher along the current curve to the time of the explosion of the EEC. To do this, we need to take more conductors, for input more energy, with a smaller diameter of each to increase the speed of energy input.
Impulse current and voltage values obtained with the explosion of conductors in the 1st experiment.
In accordance with the described reasoning, in the second experiment, 96 copper conductors with a diameter of 80 μm were taken. The total area of the cross sections of the conductors is 48⋅10−8 m2. The total mass of all conductors was about 4.3 g. The initial charging voltage was chosen as in the previous experiment, ~18 kV, respectively, the initial energy was about 15 kJ, as in the previous experiment. The explosion of the EEC occurred at 92 μs, which corresponds to the current drop and voltage peak, as illustrated in Figure 13. The maximum value of the current in the explosion was about 70 kA. In the current distribution, see Figure 13, we see again a nonpause mode of EEC transition into an electrically conductive plasma state. Further, the current increases again after several periods of high frequency oscillations to a maximum value of 127 kA, Figure 13. Then the plasma conductivity decreases due to the expansion of the plasma column, and the current begins to fall. Voltage amplitude at the moment of explosion was about 550 kV, and the electric field strength was about 550 kV/m. Voltage peak occurred approximately 0.5 μs after the explosion of conductors. The front of the voltage pulse was estimated to be approximately 100 ns. Estimates of the current density in the explosion gave a value of about 3⋅1011 A/m2. The resistance growth rate was about 16 Ω/μs. The energy inputted in the EVP during the explosion is about 60 kJ. Specific energy was 60/4.3–14 kJ/g, approximately 2.7 times more of sublimation energy of a copper.
Experimentally obtained impulse current and voltage at the load (EEC).
Characteristics of high speed camera Memrecam HX-3 (16 Gb, exposition time—200 ns, frame rate of 750,000 frame per second at working special resolution 320 × 24) allowed to fix the time of the explosion. Three frames, shot one after another, include: the first frame—before the explosion, the middle frame clearly illustrates the locally glowing area of the exploded conductor, and the next frame—a glowing plasma column formed by the products of the explosion of the EEC. The column has a radius much exceeding the initial radius of the wire, Figure 14. The split of the image is connected with the re-reflection from the neighboring conductors. Displayed glow significant attenuated on one-hundredth the frame that corresponds to time over 130 μs after the explosion (~220 μs). At this time, the current almost disappears.
Average frame illustrates of EEC explosion. The top frame is before the explosion and the bottom after the explosion.
The change of magnetic induction occurring around the EEP at the moment of break was estimated from the Ampere law in the approximation of a single turn solenoid, (N = 1),
Photo of the device for registration of current derivative from the source of powerful EMI action in the near field based on the 4th generation lightning current recorder.
There is not a ready-made model of techno economic analysis of the creation project of commercial samples of testing complexes for lightning protection tasks. We took as a basis of the economic expediency of the introduction of system of lightning protection from the point of view of minimizing outages caused by lightning. The analysis is based on the idea of calculating the internal rate of return (IRR) of the protection method, as well as the data on lightning outage presented in the report [21], University of S. Paulo, Brazil. The main numerical indicators for economic calculations and the list of lightning protection services are close to the Russian market. The methodology is based on an analysis of the cost of lightning protection and the benefits of lightning protection. The benefit was regarded as an opportunity to save on the damage caused by the emergency situation both at the power plant itself and the damage caused by the delay in the supply of electricity to consumers, including the payment of fines for regulated direct and indirect losses. It was taken into account what can be represented in monetary terms, excluding social and moral aspects. The simplified calculation did not take into account such aspects as: loss of communication, information, Internet access, reduced mobility of the population, damage to frozen products, disruption of banks, state institutions, etc. The main costs are the measures used to increase the lightning resistance of power plants. These include the improvement of insulation resistance, the use of earthed shielded cables, the installation of arresters in distribution networks, as well as the installation of grounding devices that carry out the removal of lightning currents into the ground. The volume of storm shutdowns was considered as a total number of cases and also with respect to areas with different population densities. Different protection systems with different degrees of complexity were considered. The average costs and benefits for each system were reduced to a unit of time. The obtained input data allowed estimating the values of cash flows and calculating the IRR for each protection system. This rate is equal to the ratio of profit (the amount of losses that can be avoided) to the amount of costs (investments). The following conclusions are made:
The lightning protection project is effective and appropriate if the final IRR rate is higher than the minimum rate of return for long-term financing, and for Russia, it is about 7% per annum. That is, the energy distribution company should have a positive return on investment.
Developing countries, where the cost of capital is high, face great difficulties in investing in protection systems, especially in regions with low population density and low income.
If we continue the study of technical and economic analysis in accordance with the proposed methodology in relation to the effectiveness of the implementation of the development of testing systems for our project, we can add the following conclusions:
We get the amount of benefits for the development of integrated solutions. Each benefit from the selected protection measure can be tested separately. And most importantly, it is possible to get the best comprehensive solution in the application of several protection measures. In theory, such solutions are difficult to obtain because the overall protection solution is not simply the sum of individual measures. For example, for grounding devices that perform the removal of lightning currents, to minimize the resistance of multipath grounding, it must take into account the specific resistance of the soil.
Developed complex solutions based on the tests can be standardized according to the adopted standards of protection and reproduced in the form of ready-made solutions.
By using testing complexes in the construction of power plants or scheduled safety inspections, it is possible to reduce the safe level of overvoltage and, accordingly, the number of lightning outages.
The creation and use of such test complexes are of high importance for developed countries, which have a high level of requirements for the operation of power plants, namely, to the quality and continuity of the energy supplied by them.
The cost of MIC EMG, without the option of EMI impact analysis, is approximately $ 2.3 million per 1 MJ of energy, which is transferred to the load. The evaluation was performed in 2017. As a result of R & D, a sample of testing complex, laboratory test stands, and a set of design and technological documentation necessary for the transition to commercial samples were obtained.
It should be noted that the test complexes based on EMG are a means for testing the existing protection of power stations from lightning. Despite the fact that the basis of EMG work is the physic-chemical law of conversion of chemical energy explosives into electrical energy with an efficiency of not more than 10%, and this tool is able to generate pulsed currents of the mega-ampere level and the electric field intensity of MV/m per microsecond, not achievable by other methods. In the transition from R & D to commercial facility, it requires a detailed calculation, first of all, of the technical and economic effect of the use of testing complex. The powerful impact of lightning is a probabilistic process for the power plant, but the level of destruction is much higher than any other losses. To calculate this effect, it is necessary to take specific parameters to improve lightning protection, applying to the technique described in work [22, 23], and calculate energy and exergy efficiencies. The return on investment in such complexes is likely to be correctly compared with the level of return on capital from long-term investments. There is a direct connection with the economic development of the country, which can afford high tech equipment.
Field tests of MIC EMG demonstrated stable reproduction of pulse values of currents during operation: its mobility, reliability, and safety. For the range of loads with active resistance from 1 to 10 Ω and inductance of 70–150 μH, the amplitude values of the currents ranged from 70 to 40 kA. Thus, the efficiency of application of MIC EMG for modeling lightning currents at low resistance loads such as grounding at electrical substations and power plants has been confirmed. The practical application of MIC EMG will allow increasing the level of lightning protection of strategically important energy facilities both at the stage of their building construction and during preventive inspections.
Laboratory testing of the generator of powerful EMI impacts on the basis of the EMG and electro explosive conductors in the load circuit confidently registered voltage pulses with the front of about 100 ns and the amplitude of the electric field strength of about 500 kV/m. The growth rate of the magnetic field intensity in the near field reached 0.2 T/μs. In practice, the use of such a facility will allow to solve the problem of optimizing the location of the elements of grounding devices in order to equalize the potentials in the grounding systems and to identify parasitic connections in electrical circuits, including those induced in the secondary circuits of underground utilities.
The tests of the described system samples showed the readiness of the development for the transition to commercial development. This chapter of the techno economic analysis of the development provides an approach to assess the profitability of the complex depending on the location of the power plant and the required level of lightning protection.
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The combination of electronics and computer science with biology and medicine has improved patient diagnosis, reduced rehabilitation time, and helped to facilitate a better quality of life. Nowadays, all medical imaging devices, medical instruments, or new laboratory techniques result from the cooperation of specialists in various fields. The series of Biomedical Engineering books covers such areas of knowledge as chemistry, physics, electronics, medicine, and biology. This series is intended for doctors, engineers, and scientists involved in biomedical engineering or those wanting to start working in this field.",coverUrl:"https://cdn.intechopen.com/series/covers/7.jpg",latestPublicationDate:"May 7th, 2022",hasOnlineFirst:!0,numberOfOpenTopics:3,numberOfPublishedChapters:96,numberOfPublishedBooks:12,editor:{id:"50150",title:"Prof.",name:"Robert",middleName:null,surname:"Koprowski",fullName:"Robert Koprowski",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002aYTYNQA4/Profile_Picture_1630478535317",biography:"Robert Koprowski, MD (1997), PhD (2003), Habilitation (2015), is an employee of the University of Silesia, Poland, Institute of Computer Science, Department of Biomedical Computer Systems. For 20 years, he has studied the analysis and processing of biomedical images, emphasizing the full automation of measurement for a large inter-individual variability of patients. Dr. Koprowski has authored more than a hundred research papers with dozens in impact factor (IF) journals and has authored or co-authored six books. Additionally, he is the author of several national and international patents in the field of biomedical devices and imaging. Since 2011, he has been a reviewer of grants and projects (including EU projects) in biomedical engineering.",institutionString:null,institution:{name:"University of Silesia",institutionURL:null,country:{name:"Poland"}}},subseries:[{id:"7",title:"Bioinformatics and Medical Informatics",keywords:"Biomedical Data, Drug Discovery, Clinical Diagnostics, Decoding Human Genome, AI in Personalized Medicine, Disease-prevention Strategies, Big Data Analysis in Medicine",scope:"Bioinformatics aims to help understand the functioning of the mechanisms of living organisms through the construction and use of quantitative tools. The applications of this research cover many related fields, such as biotechnology and medicine, where, for example, Bioinformatics contributes to faster drug design, DNA analysis in forensics, and DNA sequence analysis in the field of personalized medicine. Personalized medicine is a type of medical care in which treatment is customized individually for each patient. Personalized medicine enables more effective therapy, reduces the costs of therapy and clinical trials, and also minimizes the risk of side effects. Nevertheless, advances in personalized medicine would not have been possible without bioinformatics, which can analyze the human genome and other vast amounts of biomedical data, especially in genetics. The rapid growth of information technology enabled the development of new tools to decode human genomes, large-scale studies of genetic variations and medical informatics. The considerable development of technology, including the computing power of computers, is also conducive to the development of bioinformatics, including personalized medicine. In an era of rapidly growing data volumes and ever lower costs of generating, storing and computing data, personalized medicine holds great promises. Modern computational methods used as bioinformatics tools can integrate multi-scale, multi-modal and longitudinal patient data to create even more effective and safer therapy and disease prevention methods. Main aspects of the topic are: Applying bioinformatics in drug discovery and development; Bioinformatics in clinical diagnostics (genetic variants that act as markers for a condition or a disease); Blockchain and Artificial Intelligence/Machine Learning in personalized medicine; Customize disease-prevention strategies in personalized medicine; Big data analysis in personalized medicine; Translating stratification algorithms into clinical practice of personalized medicine.",annualVolume:11403,isOpenForSubmission:!0,coverUrl:"https://cdn.intechopen.com/series_topics/covers/7.jpg",editor:{id:"351533",title:"Dr.",name:"Slawomir",middleName:null,surname:"Wilczynski",fullName:"Slawomir Wilczynski",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y000035U1loQAC/Profile_Picture_1630074514792",institutionString:null,institution:{name:"Medical University of Silesia",institutionURL:null,country:{name:"Poland"}}},editorTwo:null,editorThree:null,editorialBoard:[{id:"5886",title:"Dr.",name:"Alexandros",middleName:"T.",surname:"Tzallas",fullName:"Alexandros Tzallas",profilePictureURL:"https://mts.intechopen.com/storage/users/5886/images/system/5886.png",institutionString:"University of Ioannina, Greece & Imperial College London",institution:{name:"University of Ioannina",institutionURL:null,country:{name:"Greece"}}},{id:"257388",title:"Distinguished Prof.",name:"Lulu",middleName:null,surname:"Wang",fullName:"Lulu Wang",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRX6kQAG/Profile_Picture_1630329584194",institutionString:null,institution:{name:"Shenzhen Technology University",institutionURL:null,country:{name:"China"}}},{id:"225387",title:"Prof.",name:"Reda",middleName:"R.",surname:"Gharieb",fullName:"Reda Gharieb",profilePictureURL:"https://mts.intechopen.com/storage/users/225387/images/system/225387.jpg",institutionString:"Assiut University",institution:{name:"Assiut University",institutionURL:null,country:{name:"Egypt"}}}]},{id:"8",title:"Bioinspired Technology and Biomechanics",keywords:"Bioinspired Systems, Biomechanics, Assistive Technology, Rehabilitation",scope:'Bioinspired technologies take advantage of understanding the actual biological system to provide solutions to problems in several areas. Recently, bioinspired systems have been successfully employing biomechanics to develop and improve assistive technology and rehabilitation devices. The research topic "Bioinspired Technology and Biomechanics" welcomes studies reporting recent advances in bioinspired technologies that contribute to individuals\' health, inclusion, and rehabilitation. Possible contributions can address (but are not limited to) the following research topics: Bioinspired design and control of exoskeletons, orthoses, and prostheses; Experimental evaluation of the effect of assistive devices (e.g., influence on gait, balance, and neuromuscular system); Bioinspired technologies for rehabilitation, including clinical studies reporting evaluations; Application of neuromuscular and biomechanical models to the development of bioinspired technology.',annualVolume:11404,isOpenForSubmission:!0,coverUrl:"https://cdn.intechopen.com/series_topics/covers/8.jpg",editor:{id:"144937",title:"Prof.",name:"Adriano",middleName:"De Oliveira",surname:"Andrade",fullName:"Adriano Andrade",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRC8QQAW/Profile_Picture_1625219101815",institutionString:null,institution:{name:"Federal University of Uberlândia",institutionURL:null,country:{name:"Brazil"}}},editorTwo:null,editorThree:null,editorialBoard:[{id:"49517",title:"Prof.",name:"Hitoshi",middleName:null,surname:"Tsunashima",fullName:"Hitoshi Tsunashima",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002aYTP4QAO/Profile_Picture_1625819726528",institutionString:null,institution:{name:"Nihon University",institutionURL:null,country:{name:"Japan"}}},{id:"425354",title:"Dr.",name:"Marcus",middleName:"Fraga",surname:"Vieira",fullName:"Marcus Vieira",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y00003BJSgIQAX/Profile_Picture_1627904687309",institutionString:null,institution:{name:"Universidade Federal de Goiás",institutionURL:null,country:{name:"Brazil"}}},{id:"196746",title:"Dr.",name:"Ramana",middleName:null,surname:"Vinjamuri",fullName:"Ramana Vinjamuri",profilePictureURL:"https://mts.intechopen.com/storage/users/196746/images/system/196746.jpeg",institutionString:"University of Maryland, Baltimore County",institution:{name:"University of Maryland, Baltimore County",institutionURL:null,country:{name:"United States of America"}}}]},{id:"9",title:"Biotechnology - Biosensors, Biomaterials and Tissue Engineering",keywords:"Biotechnology, Biosensors, Biomaterials, Tissue Engineering",scope:"The Biotechnology - Biosensors, Biomaterials and Tissue Engineering topic within the Biomedical Engineering Series aims to rapidly publish contributions on all aspects of biotechnology, biosensors, biomaterial and tissue engineering. We encourage the submission of manuscripts that provide novel and mechanistic insights that report significant advances in the fields. Topics can include but are not limited to: Biotechnology such as biotechnological products and process engineering; Biotechnologically relevant enzymes and proteins; Bioenergy and biofuels; Applied genetics and molecular biotechnology; Genomics, transcriptomics, proteomics; Applied microbial and cell physiology; Environmental biotechnology; Methods and protocols. Moreover, topics in biosensor technology, like sensors that incorporate enzymes, antibodies, nucleic acids, whole cells, tissues and organelles, and other biological or biologically inspired components will be considered, and topics exploring transducers, including those based on electrochemical and optical piezoelectric, thermal, magnetic, and micromechanical elements. Chapters exploring biomaterial approaches such as polymer synthesis and characterization, drug and gene vector design, biocompatibility, immunology and toxicology, and self-assembly at the nanoscale, are welcome. Finally, the tissue engineering subcategory will support topics such as the fundamentals of stem cells and progenitor cells and their proliferation, differentiation, bioreactors for three-dimensional culture and studies of phenotypic changes, stem and progenitor cells, both short and long term, ex vivo and in vivo implantation both in preclinical models and also in clinical trials.",annualVolume:11405,isOpenForSubmission:!0,coverUrl:"https://cdn.intechopen.com/series_topics/covers/9.jpg",editor:{id:"126286",title:"Dr.",name:"Luis",middleName:"Jesús",surname:"Villarreal-Gómez",fullName:"Luis Villarreal-Gómez",profilePictureURL:"https://mts.intechopen.com/storage/users/126286/images/system/126286.jpg",institutionString:null,institution:{name:"Autonomous University of Baja California",institutionURL:null,country:{name:"Mexico"}}},editorTwo:null,editorThree:null,editorialBoard:[{id:"35539",title:"Dr.",name:"Cecilia",middleName:null,surname:"Cristea",fullName:"Cecilia Cristea",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002aYQ65QAG/Profile_Picture_1621007741527",institutionString:null,institution:{name:"Iuliu Hațieganu University of Medicine and Pharmacy",institutionURL:null,country:{name:"Romania"}}},{id:"40735",title:"Dr.",name:"Gil",middleName:"Alberto Batista",surname:"Gonçalves",fullName:"Gil Gonçalves",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002aYRLGQA4/Profile_Picture_1628492612759",institutionString:null,institution:{name:"University of Aveiro",institutionURL:null,country:{name:"Portugal"}}},{id:"211725",title:"Associate Prof.",name:"Johann F.",middleName:null,surname:"Osma",fullName:"Johann F. Osma",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bSDv7QAG/Profile_Picture_1626602531691",institutionString:null,institution:{name:"Universidad de Los Andes",institutionURL:null,country:{name:"Colombia"}}},{id:"69697",title:"Dr.",name:"Mani T.",middleName:null,surname:"Valarmathi",fullName:"Mani T. Valarmathi",profilePictureURL:"https://mts.intechopen.com/storage/users/69697/images/system/69697.jpg",institutionString:"Religen Inc. | A Life Science Company, United States of America",institution:null},{id:"205081",title:"Dr.",name:"Marco",middleName:"Vinícius",surname:"Chaud",fullName:"Marco Chaud",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bSDGeQAO/Profile_Picture_1622624307737",institutionString:null,institution:{name:"Universidade de Sorocaba",institutionURL:null,country:{name:"Brazil"}}}]}]}},libraryRecommendation:{success:null,errors:{},institutions:[]},route:{name:"profile.detail",path:"/profiles/332102",hash:"",query:{},params:{id:"332102"},fullPath:"/profiles/332102",meta:{},from:{name:null,path:"/",hash:"",query:{},params:{},fullPath:"/",meta:{}}}},function(){var e;(e=document.currentScript||document.scripts[document.scripts.length-1]).parentNode.removeChild(e)}()