Chapters authored
Application of Genomic Data for PCR Screening of Bet v 1 Conserved Sequence in Clinically Relevant Plant Species
Bet v 1 is a highly immunogenic protein, which is the main cause of sensitivity to birch pollen and is described as the main birch allergen. Despite the structural similarity, Bet v 1 homologs show different properties and immunoreactivity. Here, the bioinformatic algorithms were applied for known Bet v 1 homologous nucleic acids sequences to find homology and conserved regions. Genomic sequences of PR proteins of two different fruit species, which allergens belong to PR proteins of the same type as Bet v 1, were selected to design degenerate primers. Subsequently, screening of the presence of Bet v 1 conserved genomic sequence was performed in 45 clinically relevant plant species.
Part of the book: Systems Biology
MicroRNA-Based Markers in Plant Genome Response to Abiotic Stress and Their Application in Plant Genotyping
The high conservation of miRNA sequences provided an opportunity to develop an effective type of markers that is useful not only for genetic diversity study but also as potential biomarkers in plant stress responses. The fundamental potential of miRNA-based markers relies on the primer design based on the sequences of mature miRNAs, which are part of the step-loop structures. The advantages of this marker system include high polymorphism, reproducibility, and transferability across species. The abundance of mature miRNAs, which is linked to the expression of MIRNA genes, varies greatly among miRNAs, tissue types, or developmental stages, indicating the spatially and temporally regulated expression patterns of plant miRNAs. The results confirm the significance, reliability, and the position of miRNA-based markers as stress-sensitive biomarkers, indicating their potential in a wide range of applications of agricultural research.
Part of the book: Non-Coding RNAs