Dr. Nirmal Mazumder is an Assistant Professor at Department of Biophysics, Manipal School of Life Sciences, Manipal Academy of Higher Education (MAHE), Manipal, India. He obtained his Ph.D. degree in 2013 with work on development of multimodal nonlinear optical microscopy for cell to tissue characterization from National Yang-Ming University, Taipei, Taiwan. After that, he worked in the University of Virginia, USA and Italian Institute of Technology, Genoa, Italy as a postdoctoral fellow during 2013 to 2016. He has been working in the area of development of nonlinear optical microscopes including two photon fluorescence, second harmonic generation (SHG), coherent anti-Stokes Raman scattering (CARS) for biomedical applications. He has demonstrated that using CARS microscopy, the expression of domain 2 of the HCV core protein (D2) fused to a GFP is sufficient to induce an accumulation of larger lipid droplets in the perinuclear region. Additionally, fluorescence lifetime imaging of endogenous reduced nicotinamide adenine dinucleotides [NAD(P)H], are performed, a key coenzyme in cellular metabolic processes, to monitor changes in the cofactor’s abundance and conformational state in D2-GFP transfected cells. He developed a four-channel photon counting based Stokes-polarimeter for spatial characterization of polarization effects in SHG signal and report on measurements and characterization of polarization properties of SH signals. The technique is used as contrast mechanism to characterize the polarization properties from two potassium dihydrogen phosphate (KDP) micro-crystals, collagen type-I and starch granules in SHG microscopy. This technique can be applied to investigate the molecular orientation and the degree of organization in fibrotic collagen, human dermis, keloid, cornea, microtubules as well as myosin of the skeletal muscle. Currently, he is working on the development of Stokes-Mueller based polarization microscope for tissue characterization integrated with machine learning. He is also developing a two photon fluorescence lifetime imaging system for brain imaging in freely moving mouse at hypoxic condition. He has more than 10 years of teaching experience in biophysics, biophotonics, photonics, physics and bioinformatics. His research work generated more than 35 publications in peer reviewed journals and also in conference proceedings, books, book chapters and several poster/oral/invited presentations in national and international conferences. He is also the Principle Investigator in several national and international collaborative research projects funded by Government of India. He is also working as the reviewer/guest editor/associate editor/book editor in several prestigious journals and publishers. He is a member of several national and international scientific societies and organizations including the Optical Society (OSA), SPIE—the International Society for Optical Engineering, Society of Biological Chemists (I) (Life Member) and the Environmental Mutagen Society of India (Life Member).