Microbiological quality of raw wastewater and characteristics of selected indicators (values in parenthesis indicate range of concentration).
\\n\\n
IntechOpen was founded by scientists, for scientists, in order to make book publishing accessible around the globe. Over the last two decades, this has driven Open Access (OA) book publishing whilst levelling the playing field for global academics. Through our innovative publishing model and the support of the research community, we have now published over 5,700 Open Access books and are visited online by over three million academics every month. These researchers are increasingly working in broad technology-based subjects, driving multidisciplinary academic endeavours into human health, environment, and technology.
\\n\\nBy listening to our community, and in order to serve these rapidly growing areas which lie at the core of IntechOpen's expertise, we are launching a portfolio of Open Science journals:
\\n\\nAll three journals will publish under an Open Access model and embrace Open Science policies to help support the changing needs of academics in these fast-moving research areas. There will be direct links to preprint servers and data repositories, allowing full reproducibility and rapid dissemination of published papers to help accelerate the pace of research. Each journal has renowned Editors in Chief who will work alongside a global Editorial Board, delivering robust single-blind peer review. Supported by our internal editorial teams, this will ensure our authors will receive a quick, user-friendly, and personalised publishing experience.
\\n\\n"By launching our journals portfolio we are introducing new, dedicated homes for interdisciplinary technology-focused researchers to publish their work, whilst embracing Open Science and creating a unique global home for academics to disseminate their work. We are taking a leap toward Open Science continuing and expanding our fundamental commitment to openly sharing scientific research across the world, making it available for the benefit of all." Dr. Sara Uhac, IntechOpen CEO
\\n\\n"Our aim is to promote and create better science for a better world by increasing access to information and the latest scientific developments to all scientists, innovators, entrepreneurs and students and give them the opportunity to learn, observe and contribute to knowledge creation. Open Science promotes a swifter path from research to innovation to produce new products and services." Alex Lazinica, IntechOpen founder
\\n\\nIn conclusion, Natalia Reinic Babic, Head of Journal Publishing and Open Science at IntechOpen adds:
\\n\\n“On behalf of the journal team I’d like to thank all our Editors in Chief, Editorial Boards, internal supporting teams, and our scientific community for their continuous support in making this portfolio a reality - we couldn’t have done it without you! With your support in place, we are confident these journals will become as impactful and successful as our book publishing program and bring us closer to a more open (science) future.”
\\n\\nWe invite you to visit the journals homepage and learn more about the journal’s Editorial Boards, scope and vision as all three journals are now open for submissions.
\\n\\nFeel free to share this news on social media and help us mark this memorable moment!
\\n\\n\\n"}]',published:!0,mainMedia:{caption:"",originalUrl:"/media/original/237"}},components:[{type:"htmlEditorComponent",content:'
After years of being acknowledged as the world's leading publisher of Open Access books, today, we are proud to announce we’ve successfully launched a portfolio of Open Science journals covering rapidly expanding areas of interdisciplinary research.
\n\n\n\nIntechOpen was founded by scientists, for scientists, in order to make book publishing accessible around the globe. Over the last two decades, this has driven Open Access (OA) book publishing whilst levelling the playing field for global academics. Through our innovative publishing model and the support of the research community, we have now published over 5,700 Open Access books and are visited online by over three million academics every month. These researchers are increasingly working in broad technology-based subjects, driving multidisciplinary academic endeavours into human health, environment, and technology.
\n\nBy listening to our community, and in order to serve these rapidly growing areas which lie at the core of IntechOpen's expertise, we are launching a portfolio of Open Science journals:
\n\nAll three journals will publish under an Open Access model and embrace Open Science policies to help support the changing needs of academics in these fast-moving research areas. There will be direct links to preprint servers and data repositories, allowing full reproducibility and rapid dissemination of published papers to help accelerate the pace of research. Each journal has renowned Editors in Chief who will work alongside a global Editorial Board, delivering robust single-blind peer review. Supported by our internal editorial teams, this will ensure our authors will receive a quick, user-friendly, and personalised publishing experience.
\n\n"By launching our journals portfolio we are introducing new, dedicated homes for interdisciplinary technology-focused researchers to publish their work, whilst embracing Open Science and creating a unique global home for academics to disseminate their work. We are taking a leap toward Open Science continuing and expanding our fundamental commitment to openly sharing scientific research across the world, making it available for the benefit of all." Dr. Sara Uhac, IntechOpen CEO
\n\n"Our aim is to promote and create better science for a better world by increasing access to information and the latest scientific developments to all scientists, innovators, entrepreneurs and students and give them the opportunity to learn, observe and contribute to knowledge creation. Open Science promotes a swifter path from research to innovation to produce new products and services." Alex Lazinica, IntechOpen founder
\n\nIn conclusion, Natalia Reinic Babic, Head of Journal Publishing and Open Science at IntechOpen adds:
\n\n“On behalf of the journal team I’d like to thank all our Editors in Chief, Editorial Boards, internal supporting teams, and our scientific community for their continuous support in making this portfolio a reality - we couldn’t have done it without you! With your support in place, we are confident these journals will become as impactful and successful as our book publishing program and bring us closer to a more open (science) future.”
\n\nWe invite you to visit the journals homepage and learn more about the journal’s Editorial Boards, scope and vision as all three journals are now open for submissions.
\n\nFeel free to share this news on social media and help us mark this memorable moment!
\n\n\n'}],latestNews:[{slug:"webinar-introduction-to-open-science-wednesday-18-may-1-pm-cest-20220518",title:"Webinar: Introduction to Open Science | Wednesday 18 May, 1 PM CEST"},{slug:"step-in-the-right-direction-intechopen-launches-a-portfolio-of-open-science-journals-20220414",title:"Step in the Right Direction: IntechOpen Launches a Portfolio of Open Science Journals"},{slug:"let-s-meet-at-london-book-fair-5-7-april-2022-olympia-london-20220321",title:"Let’s meet at London Book Fair, 5-7 April 2022, Olympia London"},{slug:"50-books-published-as-part-of-intechopen-and-knowledge-unlatched-ku-collaboration-20220316",title:"50 Books published as part of IntechOpen and Knowledge Unlatched (KU) Collaboration"},{slug:"intechopen-joins-the-united-nations-sustainable-development-goals-publishers-compact-20221702",title:"IntechOpen joins the United Nations Sustainable Development Goals Publishers Compact"},{slug:"intechopen-signs-exclusive-representation-agreement-with-lsr-libros-servicios-y-representaciones-s-a-de-c-v-20211123",title:"IntechOpen Signs Exclusive Representation Agreement with LSR Libros Servicios y Representaciones S.A. de C.V"},{slug:"intechopen-expands-partnership-with-research4life-20211110",title:"IntechOpen Expands Partnership with Research4Life"},{slug:"introducing-intechopen-book-series-a-new-publishing-format-for-oa-books-20210915",title:"Introducing IntechOpen Book Series - A New Publishing Format for OA Books"}]},book:{item:{type:"book",id:"3521",leadTitle:null,fullTitle:"Weed and Pest Control - Conventional and New Challenges",title:"Weed and Pest Control",subtitle:"Conventional and New Challenges",reviewType:"peer-reviewed",abstract:"This book covers alternative insect control strategies, such as the allelopathy phenomenon, tactics in integrated pest management of opportunistic generalist insect species, biological control of root pathogens, insect pest control by polyculture strategy, application of several integrated pest management programs, irrigation tactics and soil physical processes, and carbon stocks to manage weeds.",isbn:null,printIsbn:"978-953-51-0984-6",pdfIsbn:"978-953-51-5364-1",doi:"10.5772/50276",price:119,priceEur:129,priceUsd:155,slug:"weed-and-pest-control-conventional-and-new-challenges",numberOfPages:216,isOpenForSubmission:!1,isInWos:null,isInBkci:!1,hash:"66ed4d91a67af8b180cb863d339021c1",bookSignature:"Sonia Soloneski and Marcelo Larramendy",publishedDate:"March 14th 2013",coverURL:"https://cdn.intechopen.com/books/images_new/3521.jpg",numberOfDownloads:31545,numberOfWosCitations:99,numberOfCrossrefCitations:56,numberOfCrossrefCitationsByBook:1,numberOfDimensionsCitations:130,numberOfDimensionsCitationsByBook:3,hasAltmetrics:1,numberOfTotalCitations:285,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"May 29th 2012",dateEndSecondStepPublish:"June 19th 2012",dateEndThirdStepPublish:"September 23rd 2012",dateEndFourthStepPublish:"December 22nd 2012",dateEndFifthStepPublish:"January 21st 2013",currentStepOfPublishingProcess:5,indexedIn:"1,2,3,4,5,6",editedByType:"Edited by",kuFlag:!1,featuredMarkup:null,editors:[{id:"14863",title:"Dr.",name:"Sonia",middleName:null,surname:"Soloneski",slug:"sonia-soloneski",fullName:"Sonia Soloneski",profilePictureURL:"https://mts.intechopen.com/storage/users/14863/images/system/14863.jpg",biography:"Sonia Soloneski has a Ph.D. in Natural Sciences and is Assistant Professor of Molecular Cell Biology at the School of Natural Sciences and Museum of La Plata, National University of La Plata, Argentina. 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\r\n\tThis book is intended to serve as a guide and support to those undergraduate, postgraduate students, and researchers interested in understanding the process of dynamical evaluation of discrete and continuous systems with behavior from fixed points to chaos and vice-versa, and also to those who are interested in the most recent knowledge about fixed points evolution to chaos. Some dynamical systems are designated to have well-defined behavior but under certain not previewed conditions, the behavior of those systems could evolve into a very complex dynamical response. By the use of adequate mathematical tools, the complex behavior produced by unstable fixed points could be studied, characterized, and in most cases controlled. Moreover, it is well known that systems evaluated from fractional calculus models are capable to present stable, and unstable fixed points, and variations of them under parametric modification or variation in the integration order, which also could be controlled under different control mechanisms.
",isbn:"978-1-83768-436-6",printIsbn:"978-1-83768-435-9",pdfIsbn:"978-1-83768-437-3",doi:null,price:0,priceEur:0,priceUsd:0,slug:null,numberOfPages:0,isOpenForSubmission:!0,isSalesforceBook:!1,isNomenclature:!1,hash:"256aaeeb2cfa765c6a37c73e4fe7402f",bookSignature:"Dr. Guillermo Huerta-Cuellar",publishedDate:null,coverURL:"https://cdn.intechopen.com/books/images_new/12020.jpg",keywords:"Stability of Fixed Points, Banach Fixed Points Theorem, Fractional Calculus, Discontinuity of Fixed Points, Stability of Systems, Route to Chaos, Multistability, Perturbations Theory, Unstable Fixed Points, Adaptive Control, Control of Discrete-Time Chaotic Systems, Pseudo-Random Bit Generator",numberOfDownloads:null,numberOfWosCitations:0,numberOfCrossrefCitations:null,numberOfDimensionsCitations:null,numberOfTotalCitations:null,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"June 17th 2022",dateEndSecondStepPublish:"July 15th 2022",dateEndThirdStepPublish:"September 13th 2022",dateEndFourthStepPublish:"December 2nd 2022",dateEndFifthStepPublish:"January 31st 2023",dateConfirmationOfParticipation:null,remainingDaysToSecondStep:"18 days",secondStepPassed:!1,areRegistrationsClosed:!1,currentStepOfPublishingProcess:2,editedByType:null,kuFlag:!1,biosketch:"Dr. Guillermo Huerta Cuellar is a researcher in the area of nonlinear dynamics and chaotic systems, with experimental and theoretical results. Has been an author of more than 40 high-level papers, and academic editor in 4 journals, and 3 books.",coeditorOneBiosketch:null,coeditorTwoBiosketch:null,coeditorThreeBiosketch:null,coeditorFourBiosketch:null,coeditorFiveBiosketch:null,editors:[{id:"237167",title:"Dr.",name:"Guillermo",middleName:null,surname:"Huerta-Cuellar",slug:"guillermo-huerta-cuellar",fullName:"Guillermo Huerta-Cuellar",profilePictureURL:"https://mts.intechopen.com/storage/users/237167/images/system/237167.jpg",biography:"Guillermo Huerta Cuellar received a B.Sc. degree from Instituto de Investigación en\r\nComunicaciones Ópticas (IICO), from the Universidad Autónoma de San Luis Potosí, San Luis\r\nPotosí in 2004. After, he received a Ph.D. degrees from Centro de Investigaciones en Óptica\r\n(CIO), León Guanajuato, Mexico, in 2009. During 2010 to present he have been working at the\r\nExact Sciences and Technology Department in Centro Universitario de los Lagos, Universidad de\r\nGuadalajara. He has been Visiting Researcher in the department of Applied Mathematics at\r\nInstituto Potosino de Investigación Científica y Tecnológica, San Luis Potosí, México (2012-\r\n2014), in Department of Theory of Oscillations and Automatic Control, Faculty of Radiophysics,\r\nLobachevsky State University of Nizhny Novgorod, Russia (2016), sabbatical stay in the\r\nDepartment of Physics and Environmental Science at St. Mary's University, San Antonio, TX, US\r\n(2018-2019), and in División de Matemáticas Aplicadas, Instituto Potosino de Investigación\r\nCientífica y Tecnológica (IPICYT), in San Luis Potosí, S. L. P., México (2019-2020). He has\r\neditor of 3 books, and coauthor of 6 book chapters, international journals and conferences, and\r\nmore of 40 high impact publications. He is member in the National System for Researchers (SNI-\r\nCONACyT-México). He participates as Academic Editor in the Journal Complexity (2021),\r\nFrontiers in Applied Mathematics and Statistics (2022), and Axioms (2022). He has participations\r\nas a reviewer in high impact-factor journals. His research interests include study, characterization,\r\ndynamical behavior and design in nonlinear dynamical systems as lasers, electronics, and in\r\nnumerical models.",institutionString:"University of Guadalajara",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"2",totalChapterViews:"0",totalEditedBooks:"2",institution:{name:"University of Guadalajara",institutionURL:null,country:{name:"Mexico"}}}],coeditorOne:null,coeditorTwo:null,coeditorThree:null,coeditorFour:null,coeditorFive:null,topics:[{id:"15",title:"Mathematics",slug:"mathematics"}],chapters:null,productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"},personalPublishingAssistant:{id:"478197",firstName:"Veronika",lastName:"Radosavac",middleName:null,title:"Dr.",imageUrl:"//cdnintech.com/web/frontend/www/assets/author.svg",email:"veronika@intechopen.com",biography:null}},relatedBooks:[{type:"book",id:"1591",title:"Infrared Spectroscopy",subtitle:"Materials Science, Engineering and Technology",isOpenForSubmission:!1,hash:"99b4b7b71a8caeb693ed762b40b017f4",slug:"infrared-spectroscopy-materials-science-engineering-and-technology",bookSignature:"Theophile Theophanides",coverURL:"https://cdn.intechopen.com/books/images_new/1591.jpg",editedByType:"Edited by",editors:[{id:"37194",title:"Dr.",name:"Theophile",surname:"Theophanides",slug:"theophile-theophanides",fullName:"Theophile Theophanides"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"3161",title:"Frontiers in Guided Wave Optics and Optoelectronics",subtitle:null,isOpenForSubmission:!1,hash:"deb44e9c99f82bbce1083abea743146c",slug:"frontiers-in-guided-wave-optics-and-optoelectronics",bookSignature:"Bishnu Pal",coverURL:"https://cdn.intechopen.com/books/images_new/3161.jpg",editedByType:"Edited by",editors:[{id:"4782",title:"Prof.",name:"Bishnu",surname:"Pal",slug:"bishnu-pal",fullName:"Bishnu Pal"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"3092",title:"Anopheles mosquitoes",subtitle:"New insights into malaria vectors",isOpenForSubmission:!1,hash:"c9e622485316d5e296288bf24d2b0d64",slug:"anopheles-mosquitoes-new-insights-into-malaria-vectors",bookSignature:"Sylvie Manguin",coverURL:"https://cdn.intechopen.com/books/images_new/3092.jpg",editedByType:"Edited by",editors:[{id:"50017",title:"Prof.",name:"Sylvie",surname:"Manguin",slug:"sylvie-manguin",fullName:"Sylvie Manguin"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"371",title:"Abiotic Stress in Plants",subtitle:"Mechanisms and Adaptations",isOpenForSubmission:!1,hash:"588466f487e307619849d72389178a74",slug:"abiotic-stress-in-plants-mechanisms-and-adaptations",bookSignature:"Arun Shanker and B. 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In most countries agriculture represents by far the largest use of water and worldwide, it represents about 70% of total withdrawal and 90% of water consumption [1]. To address this problem wastewater reuse has proven to be an alternative to reduce anthropogenic impacts [2]. In addition, raw wastewater reuse in agriculture is a valuable tool available to developing countries to control pollution and tackle the challenge of increasing food production in water scarce areas. The benefits of reusing water in agriculture are many and beyond doubt: it saves considerable amounts of first-use water that may be assigned to critical uses; it provides nutrients that may substitute chemical fertilizers, increasing soil fertility and crop yield, and reducing production costs; it makes it possible to expand agricultural land in arid areas; it is a relatively cheap disposal method for raw wastewater; and it may avoid pollution of surface water. In addition, it has been demonstrated that the health risks of reusing water in agriculture are minimal as long as its biological quality meets established criteria [3, 4].
However, it should be considered that there are potential negative impacts that may arise, such as soil salinization as well as soil and groundwater pollution with metals and organic compounds. In addition, the use of raw wastewater for crop production poses health risks due to its microbial content, especially bacteria, viruses and parasites, which produce a wide range of diseases since many of them may survive on the environment for long periods of time.
The main issue associated to wastewater reuse is related to public health and infection risks, either real (produces a disease) or potential (transmits the infection but the disease does not develop). Infection rates may be high, low or minimal, depending on the type of pathogen, the infective dose, and the susceptibility of the affected person (host). According to epidemiological studies over the last 20 years, when untreated wastewater is applied to land for crop production, there exist real infection risks caused by pathogens. To reduce such risks, control actions must be implemented, such as treating wastewater to comply with regulation limits; developing fast, cheap, easy, and efficient detection techniques; breaking the disease-infection cycle with medical treatment; and developing education campaigns for the population [4-6].
Globally, wastewater reuse has become significant and this has encouraged many countries to develop local regulations to control water quality for reuse with the aim of reducing health and environmental risks. Due to different geographic, economic, and social characteristics, development of such regulations has been gradual and dissimilar among countries. Developed countries have worked on these regulations for several years and among them, the United States of America, and in particular the state of California, applied the first regulations on agricultural reuse in 1918 [7]. As a result, California has one of the most strict and complete regulatory frameworks. In addition, the United States Environmental Protection Agency (US EPA) has developed regulations and criteria for water reuse, which are used as a reference by many countries. Moreover, the creation of international organizations has led to the publication of general recommendations. The World Health Organization (WHO) published a series of four volumes that include information about agricultural irrigation and reuse in aquaculture [8]. At the same time, the Food and Agriculture Organization of the United Nations (FAO) has its own guidelines that are similar to those of the WHO [9]. WHO proposes limits for indicator bacteria (total or faecal coliforms) and helminth eggs when wastewater is reused for agriculture, considering that helminth eggs are highly resistant to treatment process and common in the environment.
In Europe, Mediterranean countries have detailed legislation about this topic, while countries with high water availability, such as Germany or the United Kingdom, do not regulate reuse as it is seldom practiced. Spain sets limits on faecal coliforms and nematodes based on their 1985 Water Law.
In the Americas, countries like Brazil, Costa Rica, Chile, and Mexico have made progress in developing regulations focused mainly on restricting water reuse based on microbial content, including indicator bacteria, helminth eggs and some metals, allowing organic matter and nutrients that are beneficial for agriculture to be used on land.
In Africa, despite the fact that several countries face a water crisis, many of them have lax or non-existent regulations and thus wastewater reuse is practiced uncontrolled. However, countries like Tunisia have detailed guidelines that include physicochemical and biological parameters, as well as heavy metals, an approach shared by some Mediterranean countries like Saudi Arabia, Israel, and Jordan [9].
In contrast, countries like Palestine, Libya, and Afghanistan, which have low water availability, have not been able to develop their own standards due to political conflicts and generally use either FAO or WHO guidelines. Finally, Indian regulation is outdated (1974) and limits just a few parameters, even though it has the world’s second largest population.
Usually, most regulations for reuse in irrigation establish limits on one or more of the following parameters: indicator bacteria (total or faecal coliforms), helminth eggs (intestinal worms), nematodes (a subgroup of helminthes), organic matter (as biochemical oxygen demand, BOD), dissolved and suspended solids, and heavy metals.
Demographic growth and economic development of emerging American countries has promoted the implementation of several agricultural reuse projects, some of which are summarized below.
In Mendoza, Argentina, an area known as Campo Espejo used to be irrigated with raw wastewater (2,000 ha) but currently, 129,600 m3/d originating from stabilization ponds are supplied for the irrigation of 1900 ha [10].
Chile has several successful reuse projects such as the Maipo and Maipocho regions where 130,000 ha are irrigated [11]; Antofagasta where about 20,000 m3/d of treated water are produced and 65 ha are irrigated; and Santiago de Chile with 110,000 ha that use reclaimed water mixed with first-use water [10].
In Mexico, the central and northern part of the country, where 80% of the population live, is considered arid or semi-arid, and cities like Ciudad Juarez irrigate 26,000 ha with approximately 400,000 m3/d of reclaimed water. Additionally, the Mezquital Valley, covering more than 90,000 ha, is one of the largest areas in the world where agricultural reuse is practiced [12] and where a large wastewater treatment plant (35 m3/s) is under construction to improve water quality for irrigation.
In South America, Peru encompasses a number of different biomes that range from Amazon rainforest (more than 50% of the country) to the west coast, an area with low precipitation where most of the population lives, and where small irrigation projects like San Agustin (535 ha) and Tacna (738 ha), have been developed [10].
The Tula Valley (also known as Mezquital Valley) is one of the largest areas irrigated with untreated wastewater in the world. The fact that it has been receiving wastewater since the late XIX century, and specifically for irrigation since the early XX century, makes this a unique site with regard to wastewater reuse. A large number of studies have described several processes and phenomena that emerge out of this practice, including incidental aquifer recharge, increase in crop yield, and treatment of the wastewater by the soil (in the same way as a soil-aquifer treatment system, SAT).
Concurrently, other studies have found an increase in health risks after using untreated wastewater in agriculture due to the presence of pathogens, heavy metals, and organic compounds in soil and even in groundwater. This section summarizes the history and current situation of the Tula Valley to share some lessons learned for more than 100 years of water reuse.
The Metropolitan Area of the Valley of Mexico (MAVM) has a population of 21.2 million and covers the Federal District (Mexico City) and 60 surrounding Municipalities (59 located in the State of Mexico and 1 corresponding to the State of Hidalgo). Water use is estimated to be 82 m3/s which includes 91% first use water and 9% reclaimed water. The rate of wastewater generation (including collected precipitation) ranges from 52 to 300 m3/s depending on the season, and it is conveyed to the Tula Valley. The reason for this practice is that Mexico City is located in an endorheic (closed) basin from which wastewater and excess precipitation needs to be transported to avoid flooding. On average, 60 m3/s are sent by gravity or pumping to the Tula Valley via four artificial exits (Figure 1): the Tajo de Nochistongo (deep cutting through Nochistongo Hill; 1607-1789); the Tequixquiac Tunnel (1900); the New Tequixquiac Tunnel (1955) and the Central Emitter (1975). Currently, a fifth exit (East Emitter) with a capacity of 150 m3/s is being constructed along 62 km and will be operating by 2016.
The Tula Valley is located 100 km north of Mexico City, with an elevation that ranges from 1,700 mamsl in the north, to 2,100 mamsl in the south. The climate is semiarid with an annual precipitation of 550 mm (national average is 790 mm) mainly between May and October [10] which contrasts with evapotranspiration that reaches 1,524 mm/y.
Originally, soils were low in organic matter and nutrients, and with such low precipitation, productivity was also low. By the end of the XIX Century, the Tula Valley started receiving raw wastewater from the MAVM. The first documented use of wastewater for irrigation in areas closed to the Salado River was reported around 1896. However, officially, reuse initiated in 1889 when wastewater was used to produce energy at the Juandhó and La Cañada hydroelectric plants [13], and in 1912 for irrigation [14].
Wastewater transport and use in the Tula Valley.
As wastewater generation gradually increased, the irrigated area grew from 10,000 ha in 1920, to 80,888 ha today (Figure 2). This means that about 1,350 Mm3/y are transported a distance of 98 km to the Tula Valley. Once in the Valley, wastewater is distributed through a complex hydraulic system that includes six storage dams (combined capacity: 347 Mm3), 323 km of main distribution canals, 264 km of lateral distribution canals, and 101 km of agricultural drains. Year-round availability of wastewater, as well as its organic matter and nutrient content which act as fertilizers, allowed the development of three important Irrigation Districts (ID 003, 100, and 112) that today cover more than 85 thousand hectares [15].
Growth of area irrigated and volume of water distributed since 1920 (adapted from [
The gradual growth of the productive area in the Tula Valley allowed the differentiation of regions with various irrigation ages (Figure 3). Irrigation was first performed in the south-central area of the Valley [14]. Subsequently, when the City expanded and had to dispose of a larger amount of combined wastewater, the irrigation area grew towards the north and the east following the Tula River. Subsequent expansions of the Irrigation Districts carried wastewater to the east and southeast (ID 100), where reuse had started by 1970 [16].
Areas under irrigation for different lengths of time (adapted from [
Recently, wastewater was taken to the far east of the Valley, close to the Ajacuba Municipality (ID 112). As a result, the quality of irrigation water exhibits spatial and temporal variations. For example, in the southern part of the Valley, approximately 10,000 ha are irrigated with raw wastewater, while about 35,000 ha in the central and eastern regions receive diluted wastewater (80% wastewater and 20% river water/precipitation from the Taxhimay and Requena dams) with a different composition.
On the other hand, 25,000 ha located in the far west region of the Valley are irrigated with partially treated wastewater after being stored in the Endho dam which acts as a large settling tank with a hydraulic retention time of up to three months. Finally, the northern areas use well water or return flows and are mainly utilized for growing vegetables and fruits [17].
The potential risk of bacterial, viral and parasitic diseases that can be transmitted through the human-water-soil-crop-human cycle constitutes the greatest problem associated with the use of wastewater in agriculture, for human consumption or for other uses. Thus the study of their removal is especially relevant in developing countries where they represent the higher risk of disease.
The presence of microorganisms can be a serious cause for concern. For example bacteriophages (viruses) are resistant in the environment, have been shown to have the capacity to penetrate and reach confined aquifers, and have low infective doses [7]. Cysts of
Wastewater from the MAVM reaches the distribution system at the Tula Valley without any treatment and thus it contains a high concentration of faecal coliforms (between 105 to 108 colony forming units, CFU/100 mL),
To evaluate temporal and spatial variability along the Valley, samples from different regions have been taken for microbial analyses during the wet and dry seasons. Zone 1 (south) uses raw wastewater for irrigation and has shown higher concentrations of biological indicators than Zones 2 or 3 which correspond to areas with some sedimentation and dilution (rainfall and groundwater). This variability was due to the nature of the wastewater but there was a large decrease in the
\n\t\t\t\t | \n\t\t\t\n\t\t\t\t | \n\t\t\t\n\t\t\t\t | \n\t\t
Faecal Coliforms, Log(CFU/100 mL) | \n\t\t\t6.53 ± 0.58 (5.15-7.84) | \n\t\t\tTraditional indicator of faecal contamination; may indicate the presence of pathogens; behaves similarly to other bacteria under different environmental conditions. | \n\t\t
\n\t\t\t\t | \n\t\t\t5.15 ± 1.0 (2.04-6.20) | \n\t\t\tIntestinal bacteria found in the faeces of all warm-blooded mammals; useful for indicating the quality of water for recreational use and also for reuse. | \n\t\t
\n\t\t\t\t | \n\t\t\t4.70 ± 0.81 (3.00-5.97) | \n\t\t\tAnaerobic bacteria that have been recently used as an indicator of faecal contamination; they form a resistant spore commonly found in faeces which is more resistant to disinfection and adverse environmental conditions that many pathogens; the presence of vegetative cells indicates recent contamination while spores imply prior contamination. | \n\t\t
Somatic bacteriophages, Log (PFU/mL) | \n\t\t\t3.63 ± 0.77 (2.41-6.41) | \n\t\t\tViruses that infect bacteria; used as indicators based on ease of detection by analytical laboratories; the coliphage group has been the best model for enteroviruses, given their similar physical structure and their greater resistance to treatment processes (such as chlorination); it is always present and relatively abundant in wastewater; easily detected over a short time period (24 h). | \n\t\t
\n\t\t\t\t | \n\t\t\t2,231 ± 231 (450-10,000) | \n\t\t\tPathogenic protozoa; single celled organisms that develop in two ways: as trophozoites, and as cysts; Infection results from the consumption of a mature cyst that is resistant to the gastric juices; the trophozoites can become cysts again in a process that is apparently aided by unideal luminal conditions, and they are then expelled in the faeces of persons with symptoms of the illness or with no apparent symptoms. Cysts can survive and remain active for weeks, months or even for periods of up to 7 years; capable of forming cysts (resistant structures) under adverse conditions, this organism represents a serious health risk. | \n\t\t
Helminth eggs, eggs/L | \n\t\t\t13.1±6.2 (1.8-23.0) | \n\t\t\tGroup of parasitic and free-living worms of various sizes and shapes; they cause mechanical deterioration, tissue damage, toxic effects, and blood loss; intestinal parasites cause anaemic malnutrition and/or delayed growth; they present high resistance to chemical and physical conditions and thus are considered the most resistant form of parasites; they have the ability to survive long periods of time in biosolids and soil (up to 6 years after their initial application). | \n\t\t
Microbiological quality of raw wastewater and characteristics of selected indicators (values in parenthesis indicate range of concentration).
Although it was expected that concentrations of microorganisms would be lower in the wet season due to rainfall dilution, this happens intermittently as the wet season is characterized by irregular intense storms. An additional issue is that the wastewater is mixed with stored rainwater and wastewater depending on the amount of water required by farmers and thus its quality exhibits some variability.
Concentration of selected microorganisms in different zones of the Tula Valley (Adapted from [
Emerging pollutants are defined as those unregulated pollutants that may be controlled by future regulations depending on their potential effects on health and ecosystems [24]. Advances in analytical techniques for their detection and an increased understanding of their effects on public health and the environment, has increased the need to establish discharge limits worldwide. During the last two decades, the study of emerging pollutants (such as pharmaceuticals, personal care products, and endocrine disruptors included as additives of gasoline, plastics, or detergents) in the environment has gained scientific attention. Many of these studies have been performed in aquatic ecosystems in the United States of America and Europe [24]. However, for most of the emerging pollutants there are still insufficient data to indicate their risk and ecotoxicity and thus their effects on human health or aquatic organisms remains unknown. Many of these compounds reach the environment through wastewater and they may be removed in wastewater facilities, however, some of them remain in treated effluents and enter the environment, eventually reaching groundwater [25, 26].
Initial reports on pharmaceuticals in wastewater were published in the United States in the 1970’s [27], however, even though their presence has been related to fish toxicity, little attention has been given to them [28, 29]. Considering that developed countries have detected emerging pollutants in water and wastewater, even after treatment, their presence in wastewater from the Metropolitan Area of the Valley of Mexico was clearly expected. Furthermore, the fact that many prescription drugs in developed countries are considered over-the-counter medications in Mexico, there is a higher probability of finding their active ingredients in wastewater.
On the other hand, agricultural irrigation favors the transport of pollutants, such as pesticides, from soil to groundwater. Several factors influence their incidence in groundwater, including soil permeability, the depth of the unsaturated zone, geological composition, as well as the solubility, partition (Ko/w), and dissociation (pKa) values of the pollutant. These conditions may promote their adsorption to soil particles or their leaching to groundwater. It has been reported that wastewater irrigation, treated or untreated, increases the concentration of pesticides in groundwater [30].
As mentioned previously, the presence of emerging pollutants was expected in wastewater from the MAVM, and thus at the Tula Valley, and was confirmed by different studies. Some of them [31] analyzed wastewater samples and found a variety of compounds with the exception of clofibric acid. Concentrations found were similar to those reported for wastewater samples taken at treatment plants (Table 2), but higher than those reported for their effluents [32, 33]. The concentrations of naproxen, a widely use pharmaceutical in Mexico, as well as salicylic acid were higher than those reported in other countries, while ibuprofen, diclofenac, are variable in comparison. Other compounds like 4-nonylphenol and diethylhexyl phthalate were the predominant endocrine disruptors in wastewater. Levels of Triclosan, bisphenol-A, butylbencylphtalate, estrone, and 17β-estradiol were similar to those reported elsewhere [33].
It was observed that dilution occurred during the rainy season when reported concentrations of endocrine disruptors were about half of those during the dry season. It should be noted that wastewater at the Tula Valley includes precipitation from the MAVM. Nonetheless, acid pharmaceuticals remain similar in both seasons which may suggest another phenomenon.
Siemens
\n\t\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t | \n\t\t
Ibuprofen | \n\t\t\t3,400 | \n\t\t\t- | \n\t\t\t13,100 | \n\t\t\t3,300 | \n\t\t\t2,500 | \n\t\t
Diclofenac | \n\t\t\t2,000 | \n\t\t\t2,600–5,700 | \n\t\t\t- | \n\t\t\t800 | \n\t\t\t1,607 | \n\t\t
Ketoprofen | \n\t\t\t300 | \n\t\t\t- | \n\t\t\t2,000 | \n\t\t\t500 | \n\t\t\t447 | \n\t\t
Salicylic acid | \n\t\t\t340-5,400 | \n\t\t\t- | \n\t\t\t- | \n\t\t\t- | \n\t\t\t29,867 | \n\t\t
Naproxen | \n\t\t\t440 | \n\t\t\t1,800–4,600 | \n\t\t\t4,900 | \n\t\t\t600 | \n\t\t\t13,620 | \n\t\t
In the Tula Valley, known as the largest case of indirect wastewater reuse for human consumption in the world, natural soil aquifer treatment (SAT) has been taken place for more than 100 years, recharging the aquifer and acting as a barrier to prevent contaminants from entering it. The local aquifer is being recharged at a rate of 25 m3/s due to the infiltration of untreated wastewater from unlined irrigation channels, storage dams, and excess water used for irrigation (flood irrigation practice; [12]). During percolation, natural soil infiltration occurs through unsaturated soil; as the effluent moves through the soil and the aquifer, it can undergo significant quality improvements through physical, chemical and biological processes.
Non-intentional natural SAT has treatment benefits in the unsaturated zone in the Tula Valley. It acts as a natural filter, and produces groundwater of acceptable quality due to the characteristics of the soil-aquifer structure, the residence time, and the history of the complex geohydrological system. Table 3 showed the historical data of wastewater and aquifer water quality variability obtained from studies performed at the site. It indicates that parameters related with salinity behavior are present in groundwater, in addition to parameters such as nitrate, and solids in dissolved phase. In contrast, the percentage removal of pathogenic organisms and emerging pollutants through unsaturated soil is greater than 50%.
There is evidence that during infiltration the vast majority of microorganisms are retained in the first few centimeters of soil [40], however their potential fate is influenced by size ranges of microorganisms (20-80 μm for helminth eggs, 1 μm for bacteria, 4-12 μm for protozoa), type of soil, and even soil organic matter content for bacteriophages (20 to 200 nm), and therefore for viruses; thus the migration of microorganisms through soil does not always allow complete attenuation.
\n\t\t\t\t | \n\t\t\t\n\t\t\t\t | \n\t\t\t\n\t\t\t\t | \n\t\t\t\n\t\t\t\t | \n\t\t\t\n\t\t\t\t | \n\t\t
\n\t\t\t\t | \n\t\t||||
Total Dissolved Solids, TDS | \n\t\t\tmg/L | \n\t\t\t409-1123 | \n\t\t\t546 -1586 | \n\t\t\t-55.5 | \n\t\t
Alkalinity | \n\t\t\tmg/L | \n\t\t\t324-600 | \n\t\t\t256-748 | \n\t\t\t-7.8 | \n\t\t
Nitrate (as N) | \n\t\t\tmg/L | \n\t\t\t0.02-0.47 | \n\t\t\t0.0-32 | \n\t\t\t-8014 | \n\t\t
\n\t\t\t\t | \n\t\t||||
Electrical conductivity | \n\t\t\tμS/cm | \n\t\t\t1734-3000 | \n\t\t\t1755 -4187 | \n\t\t\t-18.0 | \n\t\t
Redox potential | \n\t\t\tmV | \n\t\t\t-51 - -37 | \n\t\t\t-79.1 - -11.0 | \n\t\t\t-38.7 | \n\t\t
Sodium | \n\t\t\tmg/L | \n\t\t\t56-215 | \n\t\t\t103 -361 | \n\t\t\t-38.3 | \n\t\t
Potassium | \n\t\t\tmg/L | \n\t\t\t19-13190 | \n\t\t\t10.5 -107 | \n\t\t\t98.0 | \n\t\t
Hardness Ca | \n\t\t\tmgCaCO3/L | \n\t\t\t90-131 | \n\t\t\t70-787 | \n\t\t\t-218.4 | \n\t\t
Hardness Mg | \n\t\t\tmgCaCO3/L | \n\t\t\t12-51 | \n\t\t\t17 -301 | \n\t\t\t-65.7 | \n\t\t
Total hardness | \n\t\t\t\n\t\t\t | 122-172 | \n\t\t\t114-1006 | \n\t\t\t-181.8 | \n\t\t
Bicarbonate | \n\t\t\tmg/L | \n\t\t\t270-504 | \n\t\t\t212-652 | \n\t\t\t-12.8 | \n\t\t
Sulfate | \n\t\t\tmg/L | \n\t\t\t53-2492 | \n\t\t\t190 -3025 | \n\t\t\t-15.0 | \n\t\t
\n\t\t\t\t | \n\t\t||||
Total Bacteria count (37 ºC) | \n\t\t\tLog CFU/100 mL | \n\t\t\t6.4-7.7 | \n\t\t\t1.3-4.2 | \n\t\t\t57.6 | \n\t\t
Faecal coliforms | \n\t\t\tLog CFU/100 mL | \n\t\t\t5.6-7.5 | \n\t\t\t0.0-2.9 | \n\t\t\t70.8 | \n\t\t
Enterococci | \n\t\t\tLog CFU/100 mL | \n\t\t\t2.3-5.9 | \n\t\t\t0.0-1.9 | \n\t\t\t79.3 | \n\t\t
Clostridium spores | \n\t\t\tLog CFU/100 mL | \n\t\t\t3.2-5.6 | \n\t\t\t0.0-2.6 | \n\t\t\t61.1 | \n\t\t
Somatic Bacteriophages | \n\t\t\tLog PFU/100 mL | \n\t\t\t2.5-4.9 | \n\t\t\t0.0-2.8 | \n\t\t\t52.6 | \n\t\t
\n\t\t\t\t | \n\t\t\tCysts/L | \n\t\t\t70.3-3233 | \n\t\t\t0.0-600 | \n\t\t\t89.4 | \n\t\t
Helminth egg | \n\t\t\tOva/L | \n\t\t\t1.9-21.9 | \n\t\t\t0.0-2.9 | \n\t\t\t96.0 | \n\t\t
\n\t\t\t\t | \n\t\t||||
Clofibric acid | \n\t\t\tng/L | \n\t\t\t< LOD | \n\t\t\t< LOD-0.39 | \n\t\t\t-- | \n\t\t
Ibuprofen | \n\t\t\tng/L | \n\t\t\t1325-4700 | \n\t\t\t0.05-1.46 | \n\t\t\t99.99 | \n\t\t
Salicylic acid | \n\t\t\tng/L | \n\t\t\t13580-72979 | \n\t\t\t0.02-27.7 | \n\t\t\t99.98 | \n\t\t
2,4-D | \n\t\t\tng/L | \n\t\t\t295-2641 | \n\t\t\t< LOD-0.48 | \n\t\t\t99.99 | \n\t\t
Gemfibrozil | \n\t\t\tng/L | \n\t\t\t640-750 | \n\t\t\t< LOD-0.1 | \n\t\t\t99.99 | \n\t\t
Naproxen | \n\t\t\tng/L | \n\t\t\t5861-16336 | \n\t\t\t0.04-3.266 | \n\t\t\t100.00 | \n\t\t
Ketoprofen | \n\t\t\tng/L | \n\t\t\t82-500 | \n\t\t\t0.02-0.83 | \n\t\t\t99.89 | \n\t\t
Diclofenac | \n\t\t\tng/L | \n\t\t\t1240-3424 | \n\t\t\t< LOD-3.75 | \n\t\t\t99.98 | \n\t\t
4-nonylphenol | \n\t\t\tng/L | \n\t\t\t6970-38130 | \n\t\t\t0.81-67.6 | \n\t\t\t99.93 | \n\t\t
Pentachlorophenol | \n\t\t\tng/L | \n\t\t\t40-110 | \n\t\t\t< LOD-0.33 | \n\t\t\t99.81 | \n\t\t
Triclosan | \n\t\t\tng/L | \n\t\t\t360-2880 | \n\t\t\t< LOD-22.6 | \n\t\t\t99.83 | \n\t\t
Bisphenol-A | \n\t\t\tng/L | \n\t\t\t700-6230 | \n\t\t\t0.02-153 | \n\t\t\t99.54 | \n\t\t
Butil-bencilphthalate | \n\t\t\tng/L | \n\t\t\t125-2959 | \n\t\t\t0.03-308 | \n\t\t\t97.78 | \n\t\t
Bis-2 ethyl(hexyl)phthalate | \n\t\t\tng/L | \n\t\t\t4664-70200 | \n\t\t\t3.07-933 | \n\t\t\t99.97 | \n\t\t
Estrone | \n\t\t\tng/L | \n\t\t\t14-100 | \n\t\t\t< LOD-0.24 | \n\t\t\t99.92 | \n\t\t
17β-estradiol | \n\t\t\tng/L | \n\t\t\t6.8-22 | \n\t\t\t< LOD-0.06 | \n\t\t\t99.90 | \n\t\t
17α-etinilestradiol | \n\t\t\tng/L | \n\t\t\t<LOD | \n\t\t\t< LOD-0.05 | \n\t\t\t-- | \n\t\t
Carbamazepine | \n\t\t\tng/L | \n\t\t\t200-275 | \n\t\t\t0.14-193 | \n\t\t\t85.89 | \n\t\t
Water quality variability for wastewater and the Tula Valley aquifer and natural removal percentages (SAT)
Adapted from [23, 31, and 39] (ND: Not detected LOD: Lower limit of detection).
Data recorded over many years regarding the attenuation of emerging pollutants through soil show a mean reduction of 86 percent of the chemical load in the wastewater used for irrigation (Table 3). The main attenuation processes such as adsorption and biodegradation have been confirmed in experimental studies with local soil samples, and enriched samples [23]. The most important result of this research is the observation that large amounts of suspended and dissolved organic matter in soils [41], and even in raw wastewater, improve the adsorption of emerging pollutants in soils, and therefore their final low content in groundwater; this verifies previously published results with regard to the role of natural SAT in removing pollutants [26, 42]. In fact, the sorption behavior of three pharmaceuticals (naproxen, carbamazepine, and triclosan), a plasticizer Bis-2 ethyl(hexyl)phthalate, and the surfactant metabolite 4-nonylphenol in wastewater irrigated soil, was analyzed for different soil depths sampled from the Tula Valley, showing that the potential migration of these compounds to the aquifer depends on the physical and chemical characteristics of soil, such as organic matter content, and clay/sand percentages [43, 44].
Other studies have shown that heavy metals have accumulated in soil in the Tula Valley (for example cadmium, nickel, and lead) and their retention is associated with the length of time that wastewater has been used for irrigation [45].
It should be mentioned that a large wastewater treatment plant is currently under construction to treat the MAVM’s wastewater and reduce pollutant load to the Tula Valley. This plant, Atotonilco, is designed to treat 35 m3/s with a combined process (23 m3/s with a biological process and 12 m3/s with a physicochemical process) that may partially remove organic matter and the bulk of pathogens. However, the effect on soil attenuation must be evaluated as Gibson
Reuse in the Tula Valley has functioned as a discharge route for the closed basin of the MAVM at a relatively low cost. The characteristics of the water, in terms of organic matter and nutrients have significantly increased crop productivity in the Valley and have allowed farmers to harvest up to five crops per year of alfalfa, fodder oats, tomato, barley, and maize. Resulting yields in the Tula Valley are 71 to 150% higher than those obtained with rain-fed agriculture [19]. Thus, the contribution of nutrients represents estimated annual savings of $180 to $200 million USD. To exemplify this, agricultural production for 2011-2012 in the Irrigation Districts of the Tula Valley reached $418 million USD [46].
Since 81% of the main distribution canals as well as 52% of lateral distribution canals are unlined, 80.2 Mm3/y of conveyed wastewater infiltrates to the aquifer [47]. It is with this infiltration that wastewater quality is improved before reaching the groundwater through processes like adsorption or biodegradation, which depend on contact time and filtering distance. As an example, travel times from the irrigated fields to the springs are estimated to be 3 to 5 days with groundwater velocities of 0.02 to 6.0 m/d [16].
This infiltration has also incidentally recharged the Tula Valley aquifer for more than 100 years, at a recharge rate estimated at 25 m3/s and equivalent to 13 times the natural recharge [16]. As a consequence, the water table has risen from a depth of 60 meters in the 1950’s to 4 meters in the southern part of the Valley and several springs with flows between 40 and 600 L/s have appeared [16]. The amount of available water with a relatively good quality allows some areas to be used to grow vegetables like tomato, lettuce, cabbage, beetroot, cilantro, radish, carrot, spinach, and parsley [16]. At the same time, surface and groundwater produced by wastewater infiltration provide drinking water to approximately 500,000 people after only treatment with chlorine for disinfection [12].
In contrast, wastewater reuse in the Tula Valley has some negative impacts on the local population and the environment. Due to the pathogens and parasites contained in wastewater, the incidence of gastrointestinal diseases has increased by more than 16 times in children living in the irrigation area, compared to children unexposed to wastewater [48]. In addition, microbial and organic pollutants have been detected in the soil matrix of the irrigation areas at various depths [41, 44]. Heavy metals have accumulated in the upper soil layer [49], and Siebe [45] suggests that eventually the retention capacity of the soil might be exceeded with the risk of groundwater pollution. Moreover, the gradual salinization of local soils has caused the loss of more than 2,000 ha of cropland [17].
Even though the soil filters a large amount of pollutants from wastewater with removals above 90% [12], some studies have demonstrated that in some areas the filtered water still contains bacteria (coliforms and enterococci) and viruses [40, 50]. Filtered water presents a high concentration of salts (indicated by sodium, sulfates, carbonates, bicarbonates, chloride, calcium hardness, electrical conductivity, and potassium), and furthermore, total dissolved solids and nitrates exceed the limits set by the Mexican regulatory authorities (1,000 and 10 mg/L, respectively).
At the same time, several studies [23, 31, 40, 41, 43, 44] report the presence of emerging pollutants in springs and wells, which poses a health risk to local population (approximately 500,000 inhabitants) that use the water for human consumption (Figure 5). These pollutants exhibit low removal by conventional treatment processes and thus alternative technologies, such as membrane filtration (nanofiltration or reverse osmosis), have been proposed [51]. Different studies have demonstrated that nanofiltration removes emerging pollutants, including those with low molecular weight, in particular pesticides [52], pharmaceuticals [51], and endocrine disruptors [52].
As a result, to improve the quality of spring water in the Tula Valley, a pilot plant with a nanofiltration membrane was installed in the Cerro Colorado Spring (Figure 5) [53]; The pilot plant (11.4 m3/d) operated for 800 hours and included a pumping system, a prefilter, a softening unit for reducing potential scaling, and a nanofiltration module (Figure 6). The nanofiltration (NF) membrane was selected after laboratory trials on a pilot cell. The pilot plant had the instrumentation required to measure flow, pH, TDS, as well as pressure along the system. Recovery (permeate) was maintained at more than 66 % of the influent.
Cerro Colorado Spring at the Tula Valley.
Nanofiltration demonstrated its effectiveness for removing organic matter (92% of total organic carbon), salinity (60% of total dissolved solids and 75% of electrical conductivity) and 100% for selected microorganisms (faecal coliforms, faecal streptococci,
Flow diagram of the nanofiltration pilot plant at the Cerro Colorado Spring, Tula Valley.
Based on such studies, filtered water from the Tula Valley is being considered as a potential source for supplying drinking water for Mexico City and studies have determined that 6.5 m3/s may be sourced from local groundwater [54].
Findings confirmed by 15 years of research into the complex Tula Valley hydrological and hydraulic system, are:
Wastewater reuse for irrigation has proven to be an alternative in the water-scarce region of Tula Valley.
Agricultural irrigation in the Tula Valley using raw wastewater from the Metropolitan Area of the Valley of Mexico has been performed for more than 100 years and several studies have demonstrated that complex phenomena occur.
One of these is the natural SAT in the saturated soil zone, which even occurs today after a century of irrigation, mainly in terms of the retention of typical pathogenic organisms and emerging contaminants in wastewater of urban origin.
The observed level of natural removal through filtration by soil exceeds the expected removal levels from conventional wastewater treatment, not only for emerging contaminants, but also for microorganisms that are difficult to remove by chlorine disinfection.
Non-intentional aquifer recharge with water purification by natural infiltration, achieved through agricultural reuse of untreated wastewater, has provided new water sources for the local population with the potential to supply water for human consumption to the large city from which comes the reused wastewater originates.
Advanced treatment studies conducted by our research group have shown that it is possible to treat water from aquifer for safe water supply.
There is no doubt about the benefits of wastewater reuse for irrigation, but some potential impacts should be considered:
Even though the soil matrix retains pollutants, the limit of the soil retention capacity remains uncertain, largely for metals and emerging compounds.
The content of organic matter in soil has been a key factor in the natural attenuation of water pollution, however, the impact of future changes in the quality of water for irrigation purposes in Tula Valley, may affect removal capacity.
Soon, the Atotonilco WWTP will supply wastewater to the irrigation canals with a lower content of organic matter and nutrients. These are factors which have previously favored the natural process of the SAT and the development of agricultural activity in the area. These reductions may affect complex physicochemical processes in the future, and may have impacts on soil and groundwater quality that cannot be predicted.
The use of treated wastewater for irrigation, will allow the growing of crops normally eaten raw, which up till now has been forbidden by national legislation. Other benefits will be the reduction of direct exposure of farmers to pollutants in untreated water.
Finally, with regard to national and international legislation for irrigation, it is evident that local studies are required for establishing permissible limits. This is because existing guidelines require wastewater treatment levels which are economically onerous, mainly for developing countries, and moreover, do not include limits for other pollutants present in urban wastewater (pharmaceuticals and personal care use products). Wastewater will become more and more attractive for agricultural reuse, given the current and future problems of water scarcity.
Corn (
Corn is one of the staple foods and is used as an industrial by-product. The corn grain comes from the independent fruit called caryopsis that is inserted in the cylindrical rachis “ear”, each grain or seed is limited by the number of grains per row and rows per ear. The pericarp (wall of the ovary) and testa (seed coat) join to form the wall of the ear. The corn kernel is made up of 3 main parts: “embryo”, “endosperm” and “wall of the fruit”. The amount of kernels produced on each ear and the number of ears that grow is generally confirmed when pollinating [3, 4]. In addition to the different derivatives of corn such as corn oil, gluten, syrup, dextrose, ethanol among others. Corn starch provides ideal characteristics for various industrial applications in the textile, food, pharmaceutical, construction fields, among others. It is composed mainly of amylose/amylopectin in different proportions and polymeric organizations. These two components of starch represent approximately ≥99% of starch by dry weight. Commonly the conformation is 75% amylopectin and 25% amylose. The polymeric structure of amylopectin provides the morphology of the granule. Amylopectin is made up of -D-glucopyranosyl chains, which are highly branched and the chains are connected to each other by 1,4 bonds, with almost 6% of 1,6 bonds forming branch points. Amylose is found in small amounts compared to amylopectin. It is an unbranched unit with 1,4-linked glucopyranosyl units, although it does not have branches, but there are some molecules that are slightly branched with 1,6-linkages. Amylopectin is the main molecule that causes the various changes on the physicochemical properties of the starch granule, changing the rheological, hygroscopic, retrogradation and leaching properties of amylose, generating new structural conformations, glass transition and maximum viscosity. On the other hand, amylose (leaching) also has a directly proportional effect on the changes that amylopectin presents, due to the response factors applied to starch [5].
Starch in the native state (without amylose/amylopectin structural modification) is available as a reserve carbohydrate in many parts of plants, including roots, tubers, cereals, and seeds. Figure 1 presents a proposed scheme on the biosynthesis of corn starch. In general, the main enzymes for starch biosynthesis include mainly ADP-glucose pyrophosphorylases (AGPases), granule-bound starch synthases (GBSS), soluble starch synthases (SS), starch branching enzymes (BE) and starch debranching enzymes (DBE). AGPase catalyzes the first step reaction of starch biosynthesis by converting glucose 1-phosphate (Glc-1-P) and ATP to ADP-Glc and inorganic pyrophosphate (PPi) in amyloplasts. GBSS and SSS are responsible for synthesizing amylose and amylopectin, respectively. SBE introduces a branched structure by cleaving the internal chains of α-1,4-glucan and transferring the chain segment of six or more glucose units to the C6 position of a glucosyl residue of another glucan chain. DBEs, through their α-1,6-hydrolytic activity, act on highly branched pre-amylopectin, generating polymodal distributed end chains of amylopectin. However, recent research has shown that by modifying the plant gene, variations in the content, distribution, size and polymeric organization of amylose and amylopectin are obtained [6, 7, 8]. AGPase catalyzes the first key regulatory step in the starch biosynthetic pathways present in all higher plants that produce ADP-Glc and pyrophosphate (PPi) from Glc-1-P and ATP. Plant AGPases exist as a heterotetramer (α2β2) composed of two large (LSU) and two small (SSU) subunits with slightly different molecular masses [9, 10]. SSUs are responsible for the catalytic activity of the enzyme complex, while LSU is believed to modulate enzymatic regulatory properties that increase the allosteric response of SSU to 3-phosphoglyceric acid (3-PGA) and inorganic phosphate (Pi) [9, 11]. AGPase activity is localized to both plastids and the endosperm cytosol of cereals, in contrast to other plant species where it has been reported to occur only in plastids [12]. In a previously reported subcellular fractionation experiment using corn endosperm, the highest AGPase activity was detected in the cytosol [13]. Furthermore, the genes responsible for the shrunk2 and brittle2 starch-deficient maize kernel phenotypes encode the endosperm-specific cytosolic LSU and SSU isoforms, respectively [14, 15]. This information is an indicator that plastid AGPase, by itself, is not sufficient to support normal starch biosynthesis processes in cereal endosperm, therefore, some researchers suggest that it is possible that plastid starch phosphorylase (Pho1) play an important role in the formation of primers to complete starch biosynthesis in the endosperm. Recent advances still trying to understand the functions of individual enzyme isoforms have provided new insights into how linear polymer chains (amylose) and branched bonds (amylopectin) are synthesized in cereals. Let us remember that both polysaccharides are made up of D-glucose chains linked by α (1–4) bonds. Amylose is essentially linear with α (1–4) bonds, while amylopectin is highly branched through α (1–6) bonds. Amylopectin forms type A and B polymorphic crystals that influence the arrangement of its double helices. Type A crystals produce relatively compact helices with a lower proportion of water, while type B crystals give rise to a more open structure containing a hydrated helical nucleus. X-ray diffraction studies allow us to know this type of crystal arrangements [16]. These conformations will always be different depending on the type of botanical source (TFB), as well as the enzymes involved in the formation of amylose and amylopectin.
Corn starch biosynthesis scheme.
The functional and physicochemical properties of corn starch are influenced by the amylose/amylopectin ratio, its chain length distribution and the presence of complexes in lower proportions with lipids/proteins. In its native form, corn starch has limited applications due to its low resistance to extreme processing conditions, shear, insolubility to water at room temperature, hygroscopicity among others, which are frequently found in the industry. Therefore, at present various modification techniques have been implemented that can be achieved by enzymatic, genetic, chemical, physical methods or a combination of some of these methods, which will allow a modification, mainly on the structure of amylopectin to obtain a functional starch that can overcome deficiencies and increase its usefulness for various industrial applications [17].
The use of different techniques to modify the polymeric structure of starch unfortunately have disadvantages, it can have high costs due to the use of reagents, some processes take long periods of time, low yields can generate residues that could affect the environment. Therefore, the proposals to use starch blends that promote new physicochemical characteristics that can replace conventional methods, trends that are diversifying unique properties by combining starches from different botanical sources [18]. Physical treatments are considered ecological friendly to the environment due to the absence of chemical agents and/or concentrated alkaline/acid solutions. It’s essential to know the physicochemical properties of each starch to obtain the best combination in order to focus on the application, innovation or continuous improvement of some industrial type product.
The blends of starches with different granule size and amylose content contribute to new molecular interactions between the amylose/amylopectin contents presenting significant changes in the physicochemical properties (e.g. rheological, swelling power, gel, solubility, viscosity among others), which are attributed to the content of amylose, chain length and retrogradation [18, 19, 20].
The biofilms that are formed from starch are a very promising option to avoid the excessive use of plastic (polyethylene, vinyl chloride, polystyrene and urea formaldehyde) and low and/or no deterioration on the environment. Unfortunately, with the population and industrial increase, the use of plastics continues to increase year after year, which generates millions of tons of waste after use. Polyethylene degradation is highly influenced by the biotic and abiotic environment, thus limiting effective degradation. Currently, through various investigations, the development of plastics for packaging from biodegradable materials is being promoted, using starch as the main raw material [21, 22]. The wide variety of research on the use of starches from various botanical sources (e.g. potato, corn, wheat, tapioca, rice and others) and its low cost together with comparable characteristics for film formation have shown that it is an efficient packaging raw material and a possible substitute for polyethylene, however, there are still physicochemical properties that are still under experimentation, taking into account the permeability to water vapor, mechanical properties, glass transition and hydrophobicity. The film formation process from starch granules has been described by different authors, the quality of the film is greatly affected by the amylose/amylopectin/plasticizer (glycerol) ratio, the latter being the most widely used. The content of amylose/amylopectin, a variation in the intrinsic properties of the film can be observed due to the deviation in the content of phosphorus, molar mass of starch and the biochemistry of amyloplast and chloroplast [23]. Corn is a predominant source of starch (65%) produces a film with a higher percentage of elongation, better oxygen barrier properties and a high elastic modulus, in addition [24]. The formation of biofilms from blends of cassava/corn starch with cellulose was shown to have a hydrophobic effect by reducing the affinity of starch films with water and considerably reduced the rate of permeability to water vapor, thus improving their properties. In addition, the amylose content promotes the production of a more hydrophobic film, due to the strong interactions of intermolecular bonds with glycerol [25]. New techniques for incorporating particles onto others, as reported by Farrag, et al. [26], I present reported that they prepared starch microparticles with donut-shaped morphology from two different botanical origins (type A and C for corn and pea starch granules, respectively) by means of a simple hydroalcoholic heat treatment. The donut-shaped microparticles were loaded onto films of the same botanical origin, generating greater thermal stability of the films produced. In addition, adjusting the percentage of microparticles in the thermoplastic films allowed to supply the desired amount of oxygen and water vapor to the packaged food. This is very important to keep packaged foods fresh and healthy for as long as possible. Emulsions are systems that are characterized by presenting small dispersed droplets of an immiscible liquid phase in another liquid phase, based on many applications in different industries (eg food, nutraceutical, cosmetic, hygiene, detergents, pharmaceutical and medical), however, to stabilize these emulsifying systems, synthetic surfactants (Tweens 20/60/80) or emulsifiers of animal origin (albumin/casein) are used [27]. However, they have disadvantages in their formulation because they generate foam retention due to the trapped oxygen and interactions with other suspended molecules and even the surfactant that is not compatible, an emulsion being key for the production of various food and pharmaceutical products, it is necessary to produce emulsions with solid particles of vegetable origin to stabilize the emulsions, which is called Pickering emulsions [28]. Compared to surfactant stabilized emulsions, Pickering emulsions tend to be more stable against Ostwald ripening and coalescence. Currently, starch and cellulose are used to create Pickering emulsions. Starch for its different physicochemical properties (generally recognized as safe, non-allergenic, abundant and cheap). Unfortunately, starches are still being modified to make Pickering-type emulsions, the most widely used is succinate with octenyl succinic anhydride (OSA) reagent for emulsion production. So far, the information on Pickering starch emulsions is very scattered. The links between the manufacture of Pickering emulsions and their applications are largely absent. The lack of such links seriously undermines our research efforts to better utilize emulsions for practical applications [29]. Native starch granules are not suitable for creating stable Pickering emulsions. The starch is modified to be suitable for making a Pickering emulsion. However, future trends suggest using starch blends of granule size ≤10 μm that can be compatible with starch concentration, oil and water volume, pH, ionic strength, storage conditions, processing and presence of other components for obtain a drop size (1–100 μm) [29]. Amaranth starch has shown to have sizes ≤2 μ, which is a potential candidate to produce emulsifying systems without modifying the molecular structure by chemical agents, however, physical modifications will have to be used (e.g. temperature, pH, pressure, radiation, homogenization at high revolutions among others) to obtain favorable and applicable results [30].
Grinding on native starch granules to reduce the particle size, proved to be favorable to elaborate a Pickering emulsion system [31]. The high pressure treated starch chips and ground starch particles are partially gelatinized. They can represent a mixture of rigid particle and flexible polymer model systems in emulsions. The deformability of the starch particles can be modified in situ in Pickering emulsion systems. Heating Pickering emulsions can gelatinize starch granules to different degrees. Heating can make the boundaries between adjacent particles indistinct. During heating, some amylose and amylopectin molecules leak out of the swelling granules, causing a stabilizing layer of starch granules, becoming a layer of swollen granules interpenetrated with leached starch (amylose) polymers. In this type of emulsion systems with partially gelatinized starch granules, rigid particles (granules) and flexible polymers (leached starch molecules) coexist [32, 33]. In general, the droplet size of Pickering emulsions can be adjusted using compatible starch blends, different sizes, use of physical methods, suitable starch concentrations, and processing methods.
Extrusion is a continuous processing method, it involves high pressure and temperature in a short time. Its main function is to mix various components. The extrusion process allows the gelatinization of the starch, the denaturation of the proteins and even the molecular degradation due to the effects of high shear depending on the screw to be used, which in turn affects the physicochemical properties of the extrudates. Many studies have been conducted to explore the relationships between extrusion processing parameters and non-food starch characteristics in order to improve processing control [34]. However, little scientific evidence has attempted to understand the relationships between the physicochemical properties of extrudates and the molecular characteristics of starch after the extrusion process using starch blends, because using starches in the native state affects the bostwick flow (viscosity and/or consistency) on the extruder barrel causing a process obstruction, therefore, previous parameters for an excellent extrusion must be considered, such as the amount of starch moisture, the temperatures of each zone of the extruder, as well as the time and type shear stress, which is crucial to achieving product quality and developing novel products [35]. The use of corn starch/cassava blends provides different degrees of gelatinization and some existing air microcells in the extrudates, attributing this effect to the extension of the puff and the fine structures of the extrudates when they were exposed to different temperatures, residence times. in the extruder and the amount of moisture present in the starch sample [36].
The microencapsulation process by spray drying method allows the use of a large number of wall materials. It is essential to know the type of starch to use, it must present characteristics such as high solubility, low swelling power and viscosity, thus allowing effective encapsulation, since it can influence the properties of the emulsion, the retention of active principle, flavor and the end product shelf life. Currently there are no reports of works in which a mixture of two or more starches is implemented to be used as wall material due to the increase in viscosity, however, new trends such as using blends of porous starches, which are naturally derived from starch native by physical, chemical or biological methods. There are pores, holes and/or openings with diameters less than one micron in the structural lattices, through which molecules with smaller particle size can enter the polymeric structure and become encapsulated. The use of these possible starch blends will be an option and research topic to avoid conventional modifications on the structure of the starch, being an environmentally friendly option and without remnants of solutions with chemical reagents [37].
Corn starch is a natural biopolymer, it has multiple physicochemical properties, an option to replace most of the synthetic polymers in the future to reduce pollution and preserve the environment. The use of blends with other starches from different botanical sources and even other biopolymers of different molecular structure (e.g. pectin, cellulose, chitosan, gelatin, alginate, hydroxyapatite, protein) are tendencies to avoid the use and generation of chemical residues that affect the environment and increase production prices. These blends are promising that demonstrate in this chapter a biocompatibility and diversity of physical properties friendly with the environment without affecting third parties. The challenge to be overcome is to completely replace the most widely used synthetic polymers around the world, through the development of biofilms based on corn starch, and how to improve the mechanical, hydrophobic and permeable properties is still being investigated. In addition, cornstarch can be used to improve filtration materials, absorbents, transport, diffusibility, hydrogels, paper, adhesives, biofuels. Therefore, the future of cornstarch blends will continue to be an encouraging proposition to generate high-value products for novel applications in various areas.
The authors declare that they have no conflict of interest.
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His studies in robotics lead him not only to a PhD degree but also inspired him to co-found and build the International Journal of Advanced Robotic Systems - world's first Open Access journal in the field of robotics.",institutionString:null,institution:{name:"TU Wien",country:{name:"Austria"}}},{id:"441",title:"Ph.D.",name:"Jaekyu",middleName:null,surname:"Park",slug:"jaekyu-park",fullName:"Jaekyu Park",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/441/images/1881_n.jpg",biography:null,institutionString:null,institution:{name:"LG Corporation (South Korea)",country:{name:"Korea, South"}}},{id:"465",title:"Dr",name:"Christian",middleName:null,surname:"Martens",slug:"christian-martens",fullName:"Christian Martens",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:null},{id:"479",title:"Dr.",name:"Valentina",middleName:null,surname:"Colla",slug:"valentina-colla",fullName:"Valentina Colla",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/479/images/358_n.jpg",biography:null,institutionString:null,institution:{name:"Sant'Anna School of Advanced Studies",country:{name:"Italy"}}},{id:"494",title:"PhD",name:"Loris",middleName:null,surname:"Nanni",slug:"loris-nanni",fullName:"Loris Nanni",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/494/images/system/494.jpg",biography:"Loris Nanni received his Master Degree cum laude on June-2002 from the University of Bologna, and the April 26th 2006 he received his Ph.D. in Computer Engineering at DEIS, University of Bologna. On September, 29th 2006 he has won a post PhD fellowship from the university of Bologna (from October 2006 to October 2008), at the competitive examination he was ranked first in the industrial engineering area. He extensively served as referee for several international journals. He is author/coauthor of more than 100 research papers. He has been involved in some projects supported by MURST and European Community. His research interests include pattern recognition, bioinformatics, and biometric systems (fingerprint classification and recognition, signature verification, face recognition).",institutionString:null,institution:null},{id:"496",title:"Dr.",name:"Carlos",middleName:null,surname:"Leon",slug:"carlos-leon",fullName:"Carlos Leon",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"University of Seville",country:{name:"Spain"}}},{id:"512",title:"Dr.",name:"Dayang",middleName:null,surname:"Jawawi",slug:"dayang-jawawi",fullName:"Dayang Jawawi",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"University of Technology Malaysia",country:{name:"Malaysia"}}},{id:"528",title:"Dr.",name:"Kresimir",middleName:null,surname:"Delac",slug:"kresimir-delac",fullName:"Kresimir Delac",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/528/images/system/528.jpg",biography:"K. Delac received his B.Sc.E.E. degree in 2003 and is currentlypursuing a Ph.D. degree at the University of Zagreb, Faculty of Electrical Engineering andComputing. His current research interests are digital image analysis, pattern recognition andbiometrics.",institutionString:null,institution:{name:"University of Zagreb",country:{name:"Croatia"}}},{id:"557",title:"Dr.",name:"Andon",middleName:"Venelinov",surname:"Topalov",slug:"andon-topalov",fullName:"Andon Topalov",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/557/images/1927_n.jpg",biography:"Dr. Andon V. Topalov received the MSc degree in Control Engineering from the Faculty of Information Systems, Technologies, and Automation at Moscow State University of Civil Engineering (MGGU) in 1979. He then received his PhD degree in Control Engineering from the Department of Automation and Remote Control at Moscow State Mining University (MGSU), Moscow, in 1984. From 1985 to 1986, he was a Research Fellow in the Research Institute for Electronic Equipment, ZZU AD, Plovdiv, Bulgaria. In 1986, he joined the Department of Control Systems, Technical University of Sofia at the Plovdiv campus, where he is presently a Full Professor. He has held long-term visiting Professor/Scholar positions at various institutions in South Korea, Turkey, Mexico, Greece, Belgium, UK, and Germany. And he has coauthored one book and authored or coauthored more than 80 research papers in conference proceedings and journals. His current research interests are in the fields of intelligent control and robotics.",institutionString:null,institution:{name:"Technical University of Sofia",country:{name:"Bulgaria"}}},{id:"585",title:"Prof.",name:"Munir",middleName:null,surname:"Merdan",slug:"munir-merdan",fullName:"Munir Merdan",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/585/images/system/585.jpg",biography:"Munir Merdan received the M.Sc. degree in mechanical engineering from the Technical University of Sarajevo, Bosnia and Herzegovina, in 2001, and the Ph.D. degree in electrical engineering from the Vienna University of Technology, Vienna, Austria, in 2009.Since 2005, he has been at the Automation and Control Institute, Vienna University of Technology, where he is currently a Senior Researcher. His research interests include the application of agent technology for achieving agile control in the manufacturing environment.",institutionString:null,institution:null},{id:"605",title:"Prof",name:"Dil",middleName:null,surname:"Hussain",slug:"dil-hussain",fullName:"Dil Hussain",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/605/images/system/605.jpg",biography:"Dr. Dil Muhammad Akbar Hussain is a professor of Electronics Engineering & Computer Science at the Department of Energy Technology, Aalborg University Denmark. Professor Akbar has a Master degree in Digital Electronics from Govt. College University, Lahore Pakistan and a P-hD degree in Control Engineering from the School of Engineering and Applied Sciences, University of Sussex United Kingdom. Aalborg University has Two Satellite Campuses, one in Copenhagen (Aalborg University Copenhagen) and the other in Esbjerg (Aalborg University Esbjerg).\n· He is a member of prestigious IEEE (Institute of Electrical and Electronics Engineers), and IAENG (International Association of Engineers) organizations. \n· He is the chief Editor of the Journal of Software Engineering.\n· He is the member of the Editorial Board of International Journal of Computer Science and Software Technology (IJCSST) and International Journal of Computer Engineering and Information Technology. \n· He is also the Editor of Communication in Computer and Information Science CCIS-20 by Springer.\n· Reviewer For Many Conferences\nHe is the lead person in making collaboration agreements between Aalborg University and many universities of Pakistan, for which the MOU’s (Memorandum of Understanding) have been signed.\nProfessor Akbar is working in Academia since 1990, he started his career as a Lab demonstrator/TA at the University of Sussex. After finishing his P. hD degree in 1992, he served in the Industry as a Scientific Officer and continued his academic career as a visiting scholar for a number of educational institutions. In 1996 he joined National University of Science & Technology Pakistan (NUST) as an Associate Professor; NUST is one of the top few universities in Pakistan. In 1999 he joined an International Company Lineo Inc, Canada as Manager Compiler Group, where he headed the group for developing Compiler Tool Chain and Porting of Operating Systems for the BLACKfin processor. The processor development was a joint venture by Intel and Analog Devices. In 2002 Lineo Inc., was taken over by another company, so he joined Aalborg University Denmark as an Assistant Professor.\nProfessor Akbar has truly a multi-disciplined career and he continued his legacy and making progress in many areas of his interests both in teaching and research. 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This will pose serious problems with food, water and energy supply, particularly in less-developed countries. Considering that the human pressure over natural resources has already reached critical levels, international agencies such as the World Bank and UN Food and Agriculture Organization (FAO) are soliciting scientific research in order to identify innovative solutions to support the primary sector. Nanotechnology is a rapidly evolving field with the potential to take forward the agriculture and food industry with new tools which promise to increase food production in a sustainable manner and to protect crops from pests. Such expectations are coupled with some uncertainties about the fate of nanomaterials in the agro-environment. However, the field application of engineered nanomaterials (ENMs) has not been properly investigated yet, and many aspects have only been considered theoretically or with models, which make it difficult to properly assess the usefulness of ENMs for plant fertilization and protection.",book:{id:"6763",slug:"new-visions-in-plant-science",title:"New Visions in Plant Science",fullTitle:"New Visions in Plant Science"},signatures:"Luca Marchiol",authors:[{id:"163884",title:"Prof.",name:"Luca",middleName:null,surname:"Marchiol",slug:"luca-marchiol",fullName:"Luca Marchiol"}]},{id:"67311",doi:"10.5772/intechopen.86341",title:"Wheat Production in India: Trends and Prospects",slug:"wheat-production-in-india-trends-and-prospects",totalDownloads:2364,totalCrossrefCites:27,totalDimensionsCites:39,abstract:"Trends in Indian wheat production before and after the inception of the All India Coordinated Research Project (AICRP) on wheat have been analyzed to show its significant progress over the years. 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Miscellaneous neglected and underutilized grain legumes (MNUGLs) are crops primarily characterized by inherent features and capabilities to withstand the effects of abiotic stress and climate change, significantly replenish the soil, as well as boost food and protein security. This chapter provides insight into the benefits of MNUGLs as food and nutritional security climate smart crops, capable of growing on marginal lands. Exploring and improving MNUGLs depend on a number of factors among which are concerted research efforts, cultivation and production, as well as utilization awareness across global populace geared toward reawakening the interest on the abandoned legumes. The emergence of the clustered regularly interspaced short palindromic repeat (CRISPR/cas9) technology combined with marker-assisted selection (MAS) offers great opportunities to improve MNUGLs for sustainable utilization. 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As such, crops which are the main food source must be produced at a higher pace in order to cater in tandem with the food demand. In the past, traditional plant breeders practice classical breeding techniques to propagate plants with desirable traits. However, traditional breeding technique lies in that only individuals of the same or closely related species can be crossbred. Moreover, traditional breeders will not be able to obtain traits which are not inherent within the gene pool of their target plants through classical breeding. With recent advancements in the field of genetic engineering, it is now possible to insert beneficial genes from a completely different species or even kingdom into a target plant, yielding transgenic plants with multiple ideal traits. To develop a transgenic plant, parameters such as vector constructions, transformation methods, transgene integration, and inheritance of transgene need to be carefully considered to ensure the success of the transformation event. Hence, this chapter aimed to provide an overview of transgenic plants’ development, its advantages and disadvantages, as well as its application for the betterment of mankind.",book:{id:"6763",slug:"new-visions-in-plant-science",title:"New Visions in Plant Science",fullTitle:"New Visions in Plant Science"},signatures:"Lee-Yoon Low, Shun-Kai Yang, De-Xian Andrew Kok, Janna Ong-\nAbdullah, Ngai-Paing Tan and Kok-Song Lai",authors:[{id:"195386",title:"BSc.",name:"Shun Kai",middleName:null,surname:"Yang",slug:"shun-kai-yang",fullName:"Shun Kai Yang"},{id:"221544",title:"Dr.",name:"Kok-Song",middleName:null,surname:"Lai",slug:"kok-song-lai",fullName:"Kok-Song Lai"},{id:"240035",title:"Ms.",name:"Lee Yoon",middleName:null,surname:"Low",slug:"lee-yoon-low",fullName:"Lee Yoon Low"},{id:"240036",title:"Mr.",name:"Kok",middleName:null,surname:"Andrew-De-Xian",slug:"kok-andrew-de-xian",fullName:"Kok Andrew-De-Xian"},{id:"257891",title:"Dr.",name:"Janna Ong",middleName:null,surname:"Abdullah",slug:"janna-ong-abdullah",fullName:"Janna Ong Abdullah"},{id:"257892",title:"Dr.",name:"Ngai Paing",middleName:null,surname:"Tan",slug:"ngai-paing-tan",fullName:"Ngai Paing Tan"}]},{id:"67311",title:"Wheat Production in India: Trends and Prospects",slug:"wheat-production-in-india-trends-and-prospects",totalDownloads:2364,totalCrossrefCites:27,totalDimensionsCites:39,abstract:"Trends in Indian wheat production before and after the inception of the All India Coordinated Research Project (AICRP) on wheat have been analyzed to show its significant progress over the years. A brief intercountry comparison of productivity, production and area coupled with regional comparison within India has been attempted to give an idea about the contribution of country and regions, respectively, for global and national food security. The milestones in Indian wheat programme and research outcomes were highlighted post-AICRP along with the vision and strategies set for 2050 against diverse production challenges. Regional disparities, zone-wise production constraints and research programmes for achieving the set production target were briefed. The chapter concludes with possible interventions in strengthening the complete wheat value chain for ensuring food security for the future generation.",book:{id:"8168",slug:"recent-advances-in-grain-crops-research",title:"Recent Advances in Grain Crops Research",fullTitle:"Recent Advances in Grain Crops Research"},signatures:"Sendhil Ramadas, T.M. 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Therefore, a full understanding of plant-NP interaction and phytotoxicological mechanism is required for accurate risk assessment to ensure the safe use of nanoparticle. A range of analytical techniques have been developed to detect and characterize the uptake, translocation, cellular internalization and intracellular biotransformation of nanoparticles in plants. Imaging methodologies, including various electron microscopy, spectrometry-based techniques, together with ICP-based techniques such as ICP-OES, ICP-MS and SP-ICP-MS, have been widely used to obtain information about NPs size, morphology, size distribution, cellular localization, elemental speciation, mass concentration and so on. Due to the complexity of biological samples to be analyzed, these techniques are often combined accordingly to provide complementary information regarding plant-NP interaction. This review provides an introduction to the most widely used techniques in the study of interactions between plants and nanoparticles. 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