Relative numbers and approximate biomass of the soil microbiota in a fertile soil [8].
\r\n\t
",isbn:"978-1-83969-591-9",printIsbn:"978-1-83969-590-2",pdfIsbn:"978-1-83969-592-6",doi:null,price:0,priceEur:0,priceUsd:0,slug:null,numberOfPages:0,isOpenForSubmission:!0,hash:"e39a567d9b6d2a45d0a1d927362c9005",bookSignature:"Dr. Umar Zakir Abdul Hamid and Associate Prof. Ahmad 'Athif Mohd Faudzi",publishedDate:null,coverURL:"https://cdn.intechopen.com/books/images_new/10778.jpg",keywords:"Model-Based Control, Optimal Control, Industrial Automation, Linear Actuator, Nonlinear Actuator, System Identification, Soft Robotics, Service Robots, Unmanned Aerial Vehicle, Autonomous Vehicle, Process Engineering, Chemical Engineering",numberOfDownloads:null,numberOfWosCitations:0,numberOfCrossrefCitations:null,numberOfDimensionsCitations:null,numberOfTotalCitations:null,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"February 25th 2021",dateEndSecondStepPublish:"March 25th 2021",dateEndThirdStepPublish:"May 24th 2021",dateEndFourthStepPublish:"August 12th 2021",dateEndFifthStepPublish:"October 11th 2021",remainingDaysToSecondStep:"19 days",secondStepPassed:!1,currentStepOfPublishingProcess:2,editedByType:null,kuFlag:!1,biosketch:"Umar Zakir Abdul Hamid, Ph.D. is an autonomous vehicle expert, and with more than 30 scientific publications under his belt, Umar actively participates in global automotive standardization efforts and is a Secretary for a Society of Automotive Engineers (SAE) Committee.",coeditorOneBiosketch:"Associate Professor Dr. Ahmad 'Athif Mohd Faudzi has more than 100 scientific publications as of 2021 and is currently leading a team of 18 researchers in UTM doing research works on control, automation, and actuators.",coeditorTwoBiosketch:null,coeditorThreeBiosketch:null,coeditorFourBiosketch:null,coeditorFiveBiosketch:null,editors:[{id:"268173",title:"Dr.",name:"Umar Zakir Abdul",middleName:null,surname:"Hamid",slug:"umar-zakir-abdul-hamid",fullName:"Umar Zakir Abdul Hamid",profilePictureURL:"https://mts.intechopen.com/storage/users/268173/images/system/268173.jpg",biography:"Umar Zakir Abdul Hamid, PhD has been working in the autonomous vehicle field since 2014 with various teams in different countries (Malaysia, Singapore, Japan, Finland). He is now leading a team of 12 engineers working in the Autonomous Vehicle Software Product Development with Sensible 4, Finland. Umar is one of the recipients for the Finnish Engineering Award 2020 for his contributions to the development of all-weather autonomous driving solutions with the said firm. He is an aspiring automotive thought leader and often invited as a guest and keynote speaker to industrial and technical events. With more than 30 scientific and technical publications as author and editor under his belt, Umar actively participates in global automotive standardization efforts where he is a Secretary for a Society Automotive Engineers (SAE) Committee.",institutionString:"Sensible 4 Oy",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"0",totalChapterViews:"0",totalEditedBooks:"1",institution:null}],coeditorOne:{id:"204176",title:"Associate Prof.",name:"Ahmad 'Athif Mohd",middleName:null,surname:"Faudzi",slug:"ahmad-'athif-mohd-faudzi",fullName:"Ahmad 'Athif Mohd Faudzi",profilePictureURL:"https://mts.intechopen.com/storage/users/204176/images/system/204176.png",biography:"Assoc. Prof. Ir. Dr. Ahmad `Athif Bin Mohd Faudzi received the B. Eng. in Computer Engineering, the M. Eng. in Mechatronics and Automatic Control from Universiti Teknologi Malaysia, Malaysia and the Dr. Eng. in System Integration from Okayama University, Japan in 2004, 2006, and 2010 respectively. He was a Visiting Research Fellow at the Tokyo Institute of Technology from 2015 to 2017. From March 2019 to date, he is the Director of the Centre for Artificial Intelligence and Robotics (CAIRO), Universiti Teknologi Malaysia, Malaysia. He is mainly engaged in the research fields of actuators (pneumatic, soft mechanism, hydraulic, and motorized actuators) concentrate his work in field robotics, bioinspired robotics and biomedical applications. He is a Professional Engineer (PEng), a Charted Engineer (CEng), a member of the IEEE Robotics and Automation Society (RAS) and a member of two Akademi Sains Malaysia Special Interest Group (ASM SIG) of Biodiversity and Robotics. He is also the recipient of Top Research Scientist Malaysia (TRSM) 2020 in the area of Robotics. 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Venkateswarlu",coverURL:"https://cdn.intechopen.com/books/images_new/371.jpg",editedByType:"Edited by",editors:[{id:"58592",title:"Dr.",name:"Arun",surname:"Shanker",slug:"arun-shanker",fullName:"Arun Shanker"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"878",title:"Phytochemicals",subtitle:"A Global Perspective of Their Role in Nutrition and Health",isOpenForSubmission:!1,hash:"ec77671f63975ef2d16192897deb6835",slug:"phytochemicals-a-global-perspective-of-their-role-in-nutrition-and-health",bookSignature:"Venketeshwer Rao",coverURL:"https://cdn.intechopen.com/books/images_new/878.jpg",editedByType:"Edited by",editors:[{id:"82663",title:"Dr.",name:"Venketeshwer",surname:"Rao",slug:"venketeshwer-rao",fullName:"Venketeshwer Rao"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"4816",title:"Face Recognition",subtitle:null,isOpenForSubmission:!1,hash:"146063b5359146b7718ea86bad47c8eb",slug:"face_recognition",bookSignature:"Kresimir Delac and Mislav Grgic",coverURL:"https://cdn.intechopen.com/books/images_new/4816.jpg",editedByType:"Edited by",editors:[{id:"528",title:"Dr.",name:"Kresimir",surname:"Delac",slug:"kresimir-delac",fullName:"Kresimir Delac"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"3621",title:"Silver Nanoparticles",subtitle:null,isOpenForSubmission:!1,hash:null,slug:"silver-nanoparticles",bookSignature:"David Pozo Perez",coverURL:"https://cdn.intechopen.com/books/images_new/3621.jpg",editedByType:"Edited by",editors:[{id:"6667",title:"Dr.",name:"David",surname:"Pozo",slug:"david-pozo",fullName:"David Pozo"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}}]},chapter:{item:{type:"chapter",id:"46266",title:"The Effect of Industrial Heavy Metal Pollution on Microbial Abundance and Diversity in Soils — A Review",doi:"10.5772/57406",slug:"the-effect-of-industrial-heavy-metal-pollution-on-microbial-abundance-and-diversity-in-soils-a-revie",body:'Metals are essential components of the ecosystem, whose biologically available concentrations depend mainly on geological and biological processes [1]. There are several definitions of heavy metals, and some of them are based on the mass density of these elements. Authors of numerous publications use different limits to define the threshold density for a “heavy metal”, ranging from 3.5 to 7 g×cm-3, however, the majority of authors suggests that the mass density of heavy metals should be greater than 4.5 g×cm-3 [2]. Within the group of heavy metals one can distinguish both elements that are essential for living organisms (microelements) and the elements whose physiological role is unknown and thus they are “inactive” towards organisms. The metals that serve as microelements in living organisms usually occur in trace amounts, precisely defined for each species and both their deficiency and excess badly affect living organisms [3]. The term “heavy metal” is linked in many people’s minds to metals that are toxic. However, this is not always the truth. The effect of any substance on a living system is always dependent on its available concentration to cells. Also, several heavy metal ions are crucial in metabolic processes at low concentrations but are toxic at high concentrations [2]. Nevertheless, locally elevated levels of these elements can create significant environmental and health problems when the release of metals through various biological, geological and anthropogenic processes far exceeds its natural content resulting from processes of metal cycling. Heavy metal pollution of terrestrial environments is of great concern, due to the persistence of metals in the ecosystem and their threat to all living organisms [4].
Given the importance of the subject of soil heavy metal pollution and its effect on soil microorganisms, this chapter gives an overview of the severity of the problem when it comes to the reaction of soil microbial community to the environmental pollution. The first part of this chapter deals with the abundance of microorganisms in soils and their role in this environment. The next part concerns major sources of heavy metals in soils with particular emphasis on the most important source of soil pollution, i.e. human activity (and more precisely – industry and mining). The following part discusses the effects that toxic levels of heavy metals may have on the microbial population in soils. The last two parts of this chapter describe the ways of dealing with heavy metal pollution – one introduces the term of phytoremediation (soil remediation with the use of plants) and the other one focuses on the use of microorganisms resistant to heavy metals in the process of soil remediation.
Except for occasional insects or earthworms, once visible traces of plant biomass are removed, soil appears as a lifeless mass, that is composed of mineral particles and organic residues. However, even desert soils are abundant source of living microorganisms. This seemingly lifeless matter contains complex microbial community, including bacteria, fungi, protozoa and viruses. The integrity of the aboveground and belowground ecosystems depends on the stability, resilience and function of the soil microbial community [5].
Soil is an interesting medium for growing microorganisms, as it contains various nutrients that the microbes need for their metabolism. Unfortunately, nutrients are not always readily available [6]. However, it is one of the richest reservoirs of microorganisms, i.e. 1 gram of agricultural soil may contain even several billion colony forming units (CFUs) of microorganisms belonging to thousands of different species [7], and even though microorganisms constitute less than 0.5% of the soil mass, they have a major impact on soil properties and processes [5]. Table 1 presents the average numbers of soil microorganisms in a “typical” temperate soil. Destruction of the soil microbiota through mismanagement or environmental pollution causes decline or even death of the aboveground plant and animal populations.
\n Organisms\n | \n\n Numbers\n | \n\n Biomass [wet kg×ha-1]\n | \n|
\n Per m2\n | \n\n Per g\n | \n||
Bacteria | \n1013-1014 | \n108-109 | \n300-3000 | \n
Actinomycetes | \n1012-1013 | \n107-108 | \n300-3000 | \n
Fungi | \n1010-1011 | \n105-106 | \n500-5000 | \n
Microalgae | \n109-1010 | \n103-106 | \n10-1500 | \n
Relative numbers and approximate biomass of the soil microbiota in a fertile soil [8].
The most characteristic feature of microbial habitats is the great micro-spatial variability of environmental parameters, like temperature or nutrient availability. Many basic requirements of heterogeneous microorganisms are satisfied by various soil microhabitats. This is the reason why, in ecological terms, a number of varying microbial niches can be described. Therefore, the microbial community is composed of diverse taxa with different nutritional demands within small microenvironments [9]. Analysis of the spatial distribution of bacteria at microhabitat levels showed that in soils subjected to different fertilization treatments, the majority of bacteria were located in micropores of stable soil micro-aggregates (2 – 20 µm), as they contained over 80% of cells [10]. Such microhabitats offer the most favorable conditions for microbial growth in terms of water and substrate availability, gas diffusion and protection against predation. The microhabitat-adapted groups of microorganisms form so-called consortia which are held together by mutually facilitating metabolic processes. The consortia are characterized by more or less sharp boundaries, and variable level of interaction with each other and with other parts of the soil biota. Numerous investigations emphasize the impact of soil structure and spatial isolation on microbial diversity and community structure [11]. Some studies indicate that the soil particle size affects the diversity of microorganisms and community structure to a greater extent than other factors such as bulk pH and the type or amount of available organic compounds [12]. Other investigations show that the type and amount of available organic substrates strongly affect the abundance of microbial groups and their functional diversity in soils [13]. Fierer and Jackson [14] claim that the structure of soil bacterial communities is not random also at continental scale and that the diversity and composition of soil bacterial communities at large spatial scales can be predicted to a large extent by a single variable, that is soil pH. The diversity of soil microorganisms comprises different levels of biological organization. It includes genetic variability among taxa (species), number (richness), relative abundance (evenness) of taxa and functional groups within communities [11]. The overall biodiversity of soil microflora comprises bacteria, fungi, actinomycetes and photosynthetic microorganisms [6].
Bacteria constitute the most numerous group of soil microbes – a teaspoon of productive soil contains between 100 million and 1 billion bacterial cells. As soil environment changes rather drastically, spore-forming bacteria tend to be the most common. When environmental conditions become too difficult for normal growth, the bacteria form spores and remain dormant until the environment returns to proper conditions [6]. They facilitate various processes in soils, e.g. those related to water dynamics, nutrient cycling or disease suppression [15]. Soil-dwelling bacteria may be divided into different groups based on:
Shapes: rods (also called bacilli), sphere (also called cocci) and spiral (also called spirilla)
Their reaction to oxygen: aerobic (bacteria that need oxygen for their survival) and anaerobic (the ones that do not require oxygen and in most cases cannot bear oxygen that is deadly for them)
Result of Gram staining: Gram negative (stain pink and have thinner cell walls, they are the smallest ones and tend to be more sensitive to water stress) or Gram positive (stain violet, have thicker cell walls, are larger in size and tend to resist water stress)
Source of carbon they use: autotrophs (obtain carbon from carbon dioxide – some autotrophic bacteria directly use sunlight in order to produce sugar from carbon dioxide, while others depend on various chemical reactions) or heterotrophs (they obtain carbohydrates from their environment)
Classification based on phyla: based on morphology, barcode DNA sequences, physiological requirements and biochemical characteristics, bacteria have been classified into 12 phyla. Each phylum corresponds to a number of bacterial species and genera [15].
Tate [5] lists the most commonly encountered soil bacterial genera as: Acinetobacter, Agrobacterium, Alcaligenes, Arthrobacter, Bacillus, Brevibacterium, Caulobacter, Cellulomonas, Clostridium, Corynebacterium, Flavobacterium, Hyphomicrobium, Metallogenium, Micrococcus, Mycobacterium, Pseudomonas, Sarcina, Streptococcus and Xanthomonas. These are the heterotrophic bacteria that are augmented in soil by autotrophic and mixotrophic representatives, including nitrifiers, Thiobacillus species and iron bacteria.
Bacteria facilitate a number of physical and biochemical alterations or reactions in soils and thereby directly or indirectly support the development of higher plants. Their performance is vital for a variety of processes that include: decomposition of cellulose or other carbohydrates (e.g. Bacillus, Achromobacter, Cellulomonas, Clostridium, Methanococcus), ammonification (Bacillus, Pseudomonas), nitrification (Nitrosomonas, Nitrobacter), denitrification (Achromobacter, Pseudomonas, Bacillus, Micrococcus) and nitrogen fixation (symbiotic Rhizobium, Bradyrhizobium etc., non-symbiotic Azotobacter, Beijerinckia) [16].
On the other hand, soil fungi form three functional groups: decomposers, mutualists and pathogens. Fungi, along with bacteria, are important decomposers of hard to digest organic matter and they increase nutrient uptake of phosphorus. Mycorrhizal fungi support plants by promoting root branching and increasing nitrogen, phosphorus and water uptake. They improve plant resilience to pests, diseases or drought and improve soil structure, as fungal hyphae binds soil particles together to create water-stable aggregates. They in turn create the pore spaces in the soil that enhance water retention and drainage [17]. The most common fungi found in soil belong to the Penicillium and Aspergillus genera together with the representatives of the Zygomycetes and the mycorrhizae-associated Ascomycetes and Basidiomycetes [5].
Actinomycetes are a large group of microorganisms, systematically identified as bacteria, that grow as hyphae. They decompose a wide range of substances, but they are particularly important in degrading recalcitrant (difficult to degrade) compounds such as chitin, lignin, keratin and cellulose. Moreover, they produce a number of secondary metabolites such as antibiotics i.e. streptomycin [18] or geosmine which is responsible for “earthy” smell after soil plowing [15]. Actinomycetes are important in forming stable humus, which enhances soil structure, improves soil nutrient storage and increases water retention in soils. According to Tate [5], the most commonly encountered soil actinomycetes belong to Nocardia and Streptomyces genera.
Algae are the most common among photosynthetic microorganisms found in soil. They are found only near soil surface, where light is readily available [6]. The most common genera of green algae found in soil are: Chlorella, Chlamydomonas, Chlorococcum, Protosiphon etc. and that of diatoms are Navicula, Pinnularia. Synedra, Frangilaria. Their functions include the maintenance of soil fertility, increasing water retention capacity of soil, prevention of soil erosion due to the fact that they act as cementing agents in binding soil particles. They add organic matter to soil after the cell death and thus increase the amount of organic carbon, while their photosynthetic activity release large quantity of oxygen that facilitate the aeration in submerged soils or oxygenate the soil environment. They also take part in weathering rocks, thus building up the soil structure [19]
Although biomass of all microorganisms living in soil constitutes only several percent of organic matter content, they play an important role in the functioning of entire ecosystems [20]. They take part in soil formation, mineralize organic substances, provide plants with bioavailable compounds, cooperate with plants or may be used as a source of insecticidal substances [21]. One of the most important and most widely studied microbial groups in terms of beneficial effects to soil and plants is the group of Plant Growth Promoting Rhizobacteria (PGPR) [22]. This group includes bacterial species from genera such as Azotobacter, Azospirillum, Bacillus, Burkholderia, Enterobacter, Erwinia, Flavobacterium, Pseudomonas and Rhizobium [23]. Activity of these bacteria significantly increases plant growth and yield due to a variety of mechanisms, such as phytohormone production, symbiotic and asymbiotic N2 fixation, production of siderophores, activity against phytopathogenic microorganisms, synthesis of antibiotics, enzymes and/or fungicidal compounds, as well as solubilization of mineral phosphates and other nutrients [24]. PGPR may improve plant growth, salinity and metal toxicity stress tolerance, as well as they are able to produce phytohormones such as indole-3-acetic acid (IAA) [25]. Some PGPR produce the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase, which hydrolyses ACC, the immediate precursor of ethylene in plants. By decreasing its concentration in seedlings and thus its inhibitory effect, these PGPR stimulate seedlings’ root length [26]. Figure 1. shows the ways how Plant Growth Promoting Rhizobacteria can stimulate plants. Bacteria from the genus Rhizobium form symbiotic associations with roots of leguminous plants like clovers, peas or alfalfa. These Gram-negative, rod-shaped bacteria infect growing root hairs, forming visible nodules. In this form of symbiosis, plants supply simple carbohydrates to bacteria while bacteria convert nitrogen (N2) from air into the forms (NO3- or NH4+) that plant can use. When leaves or roots from the plant decompose, nitrogen content increases in soil [15]. Some microbial species are capable of detergent decomposition, taking part in self-purification process of soils. Decomposers are particularly important in immobilizing or retaining nutrients in their cells, thus preventing the loss of nutrients, such as nitrogen, from the rooting zone.
Summary of mechanisms employed by Plant Growth Promoting Rhizobacteria to stimulate plant development.
Despite beneficial effects of numerous soil microbes on plant growth or development, soil structure and functioning, some soil-dwelling microorganisms may cause plant, animal and human diseases. Similarly to the beneficial soil microflora, soil pathogens include bacteria, fungi and viruses. One of the example of the most important or best known plant pathogens include Agrobacterium tumefaciens (whose updated scientific name is now Rhizobium radiobacter) [27] which is the causal agent of crown gall disease of walnuts, grape vines, stone fruits and many others. These bacteria infect plant roots and induce cells to divide (due to overproduction of auxin and cytokinin), causing a tumor-like swellings that contain infected cells [28]. Erwinia carotovora (or now called Pectobacterium carotovorum) and Erwinia amylovora, the Gram-negative plant pathogens with a diverse host range cause infections of numerous agriculturally and scientifically important plant species, such as potato, apple, pear and some members of the family Rosaceae [29]. Soil is also an abundant source of fungal pathogens. Among them we may distinguish Rhizoctonia solani, a plant pathogenic fungus with a wide host range and worldwide distribution. It causes collar rot, crown rot, root rot, damping off and wire stem [30]. It mainly attacks plant seeds below the soil surface, but may also infect leaves and stems. Due to a variety of hosts that this pathogen attacks, it is of great importance and is detrimental to a variety of crops. The Armillaria root rot, caused by several species of basidoimycete genus Armillaria – the honey fungus is, on the other hand, one of the greatest threat for woody plants [31]. Another example of soil-borne plant pathogens is an important genus of fungi – Fusarium, which contains a number of, worldwide distributed, phytopathogenic species [32]. Moreover, Fusarium has also been more recently reported as an emerging human pathogen for immunocompromised patients [33]. Clostridium tetani is an example of one of the most dangerous soil-borne human pathogens. It is a tetanus-causing Gram-positive bacterium, whose transmission occurs through the contamination of wounds with soil carrying its spores [34]. Generally, soil is a typical carrier of human bacterial and fungal pathogens. Another example of them is Bacillus anthracis, the causative agent of anthrax, which is found worldwide in a variety of soil environments. Inhalation of B. anthracis spores can be fatal. Nevertheless, the incidence of both of these fatal diseases has been largely controlled in developed countries due to the development of vaccines [35].
Undoubtedly, soil is an inexhaustible reservoir of microorganisms, both beneficial and pathogenic ones. Causing the imbalance between groups of soil macro- and microorganisms may be irreversible and result in a variety of effects, sometimes unpredictable. Such imbalance may be caused by soil pollution resulting from developing industry, therefore understanding the sources and effects of industrial soil pollution is an important element in preventing the environmental degradation.
Chemical compounds, entering the ecosystem as a result of different human activities, may accumulate in soil and water environments. Therefore, soil may be regarded as a long-term reservoir of pollutants, from which these compounds may be introduced to food chains or groundwater [36]. Inappropriate and careless disposal of industrial waste often results in environmental pollution. The pollution includes point sources such as emission, effluents and solid discharge from industry, vehicle exhaustion and metal smelting or mining, as well as nonpoint sources (e.g. the use of pesticides or excessive use of fertilizers) [37]. Each of the sources have their own damaging effects on plant, animal and human health, but those that add heavy metals to soils are of serious concern due to the persistence of these elements in the environment. They cannot be destroyed, but are only transformed from one state to another [38].
Soil pollution may be defined as presence of xenobiotics (e.g. chemical compounds, radioactive elements) that alters the soil properties – both chemical, physical and biological. Soil pollution, including heavy metals, may be of natural origin, like volcanic eruptions, animal excrements or ore leaching. Nevertheless, human activity and mostly chemical industry, mining and metallurgy, as well as municipal management and traffic emissions are the main source of environmental pollution. Some authors also mention that waste disposal, waste incineration, fertilizer application and long-term application of wastewater in agricultural lands may result in heavy metal pollution of soils [39].
Heavy metals occur naturally in soils due to pedogenetic processes of weathering parent materials, however concentrations of these metals are regarded as trace (<1000 mg×kg-1) and rarely toxic [40]. Due to the disturbance and acceleration of the natural slow geochemical cycles of metals by man, most soils of rural and urban environments accumulate one or more heavy metals above the defined background levels, high enough to cause risks to ecosystems [41]. Nevertheless, heavy metals occurring in soils from anthropogenic sources tend to be more mobile, therefore more bioavailable than pedogenic or lithogenic ones [42].
Communication routes, such as roads, railways etc., are an important source of soil pollution, especially in the case of lead and zinc. Despite restricted use of leaded gasoline adopted in most countries, lead remains one of the most serious automotive-originating metal pollutant. The areas located nearby roads, particularly in urban sites, are the most vulnerable to automotive pollution. Apart from lead and zinc, chromium, cadmium, nickel and platinum are among the pollutants emitted by combustion engine-powered vehicles [43]. Heavy metals enter the environment as a result of tire wear and damage of vehicle parts. Moreover, grease used in vehicles may also be the source of cadmium pollution along roads [44]. Nickel emission results from this metal being added in gasoline and atmospheric abrasion of nickel-containing parts of automobiles [45]. The changes in the concentrations of lead, nickel, cadmium, copper and zinc in roadside soils are frequently attributed to traffic density [46].
Standard agricultural practices are also a significant source of heavy metals in soils, as application of fertilizers and pesticides has contributed to a continuous accumulation of these elements. Heavy metals can accumulate in soils due to the application of liquid and solid manure, as well as inorganic fertilizers [47]. The application of numerous biosolids, such as livestock manures, composts and municipal sewage sludge on agricultural soils leads to the accumulation of various heavy metals, such as, Cd, Cr, Cu, Hg, Mo, Ni, and Zn [48]. Lime and superphosphate fertilizers contain not only major elements necessary for plant nutrition and growth but also trace metal impurities such as cadmium. The presence of high concentrations of Cd in some fertilizers (particularly in phosphatic fertilizers) is of most concern due to the toxicity of this metal and its ability to accumulate in soils as well as due to its bioaccumulation in plant and consequently in animal tissues [49, 50]. Additionally, copper-containing compounds have been widely used in agricultural practice as pesticides. Copper oxychloride is annually applied on vineyards as a fungicide to control a significant number of plant diseases. Inevitably, this Cu ends up in the agricultural soil and adjacent pristine natural vegetation [51]. Lead arsenate was used in fruit orchards for many years to control some of the parasitic insects. Arsenic-containing compounds were also extensively used to control pests in banana plantations in new Zealand and Australia [52]. High fertilizer applications and acid atmospheric deposition, combined with insufficient liming, may also cause a decrease in pH and thus increase heavy metal bioavailability, aggravating the problem of deteriorating food quality, metal leaching and impact on soil organisms [53]. The application of municipal wastewater or industrial waste as fertilizers and liming agents in agriculture is a separate issue. Application of this type of waste requires constant monitoring of the amount and proportion of harmful factors, including heavy metals. The high risk of soil pollution with Cd, Zn, Ni and Pb as a result of industrial waste application as fertilizers was also evidenced [50].
Airborne sources of heavy metals include stack emissions or fugitive emissions such as dust from storage areas or waste heaps. Stack emissions can be distributed over a wide area by natural air currents, while fugitive emissions are often distributed over much smaller areas. In general, concentrations of pollutants are much lower in fugitive emissions compared to stack emissions. The type and concentration of metals emitted from both types of sources depend on site-specific conditions. All solid particles in smoke from fires and other emissions from factory chimneys are deposited on land or sea. Most forms of fossil fuels contain some heavy metals and this form of environmental pollution has been increasing since the industrial revolution began. For instance, very high concentrations of Cd, Pb and Zn have been found in plants and soils adjacent to smelting plants. Another major source of soil pollution is the aerial emission of lead from combustion of petrol containing tetraethyl lead; this contributes substantially to the content of Pb in soils in urban areas and in those adjacent to major roads [52].
Another, and one of the most significant sources of heavy metal pollution of soils, includes heavy industry, e.g. mining and metallurgy. Industrial airborne heavy metal contamination of the nonferrous smelters surrounding landscapes is a well-known and widely occurring phenomenon. Emissions of metallurgical dust are spread according to the wind direction and particle size while soil is the main receiver of heavy metals in dry land. Dust emissions from smelters using sulfide copper-nickel ores are similar, regardless of their location, owing to the fact that the same raw materials are used in metallurgical processes. The following major metal-containing compounds are deposited onto the landscape in the form of dust emissions from smelters: pentlandite (Ni,Fe)9S8, pyrrotite Fe7Sg(Nix), chalcopyrite CuFeS2, chalcosite Cu2S, covellite CuS, cuprite Cu20, tenorite CuO, and metal copper and nickel [54]. Surface soil layers in the mining or metallurgy areas are often heavily polluted with copper. In the vicinities of steel plants the concentration of this element exceeds several thousand ppm and the pollution remains for a long time, even after the operation of mines or steel plants had been stopped [50]. The fine fractions of dust are enriched with lead, arsenic, and zinc. The quantity and composition of dust derived from different sources (metallurgical processes) varies according to the raw materials and the condition of the gas cleaning systems [54]. The cause for the frequently widely dispersed metal pollution in habitats of mining areas was found in the formation of acid mine drainage (AMD). The runoff from mining heaps of active and abandoned mines can be extremely acidic, with pH values reaching as low as pH 2 [9]. Chemical and biological oxidation of the abundant mineral pyrite (FeS2) occurs after the unearthing of pyrite-containing rock formations and results in an acidification of the dump material [55]. Under acidic conditions, the majority of heavy metals is leached from the waste dump and they are transported as AMD in streamwaters [9]. Galvanization industry may cause soil pollution with silver as well as other industrial facilities that use silver salts. Additionally, the increased amount of silver may by introduced to soils with municipal sewage. Municipal sewage contains also large amounts of highly soluble forms of zinc, which may then easily contaminate soil environment [50]. Zinc is also extensively used in metallurgical industry, as an anti-corrosion agent in alloys and in galvanization. It is frequently used in paint industry [50]. The concentration of cadmium highly increases in soils polluted with emissions from nonferrous metal plants, which constitute over 60% of all anthropogenic sources of this element in soils. Municipal sewage contains on average 10 – 40 ppm of cadmium, while industrial sewage may contain over 1000 ppm. This is also a case of large amounts of lead that may be introduced into soils from municipal sewage and waste, as they contain mobile forms of this element. This may result in large increase in the concentration of lead in soils that may exceed several times the admissible limits. Additionally, dust emissions from landfills of nonferrous metal plants may become dangerous sources of lead in soils [50]. Table 2 shortly summarizes the major sources of different heavy metals in soil.
\n Heavy metals\n | \n\n Sources\n | \n
As | \nSemiconductors, petroleum refining, wood preservatives, animal feed additives, coal power plants, herbicides, volcanoes, mining and smelting | \n
Cu | \nElectroplating industry, smelting and refining, mining, biosolids | \n
Cd | \nGeogenic sources, anthropogenic activities, metal smelting and refining, fossil fuel burning, application of phosphate fertilizers, sewage sludge | \n
Cr | \nElectroplating industry, sludge, solid waste, tanneries | \n
Pb | \nMining and smelting of metalliferous ores, burning of leaded gasoline, municipal sewage, industrial wastes enriched in Pb, paints | \n
Hg | \nVolcano eruptions, forest fire, emissions from industries producing caustic soda, coal, peat and wood burning | \n
Se | \nCoal mining, oil refining, combustion of fossil fuels, glass manufacturing industry, chemical synthesis (e.g., varnish, pigment formulation) | \n
Ni | \nVolcanic eruptions, land fill, forest fire, bubble bursting and gas exchange in ocean, weathering of soils and geological materials | \n
Zn | \nElectroplating industry, smelting and refining, mining, biosolids | \n
Different sources of heavy metals in soils [56]
Metals without biological function are generally tolerated only in minute concentrations, whereas essential metals with biological functions, are usually tolerated in higher concentrations [9]. They have either metabolic functions as constituents of enzymes or meet structural demands, e.g. by supporting the cell envelope. Frequently the concentration and the speciation of metal determine whether it is useful or harmful to microbial cells [9].
Microorganisms are the first biota that undergoes direct and indirect impacts of heavy metals. Some metals (e.g. Fe, Zn, Cu, Ni, Co) are of vital importance for many microbial activities when occur at low concentrations. These metals are often involved in the metabolism and redox processes. Metals facilitate secondary metabolism in bacteria, actinomycetes and fungi [9; 57]. E.g. chromium is known to have stimulatory effect on both actinorhodin production and growth yield of the model actinomycete S. coelicolor [58]. However, high concentrations of heavy metals may have inhibitory or even toxic effects on living organisms [59]. Adverse effects of metals on soil microbes result in decreased decomposition of organic matter, reduced soil respiration, decreased diversity and declined activity of several soil enzymes [60]. Some of the general changes in morphology, the disruption of the life cycle and the increase or decrease of pigmentation are easy to observe and evaluate [9]. Rajapaksha et al. [61] compared the reactions of bacteria and fungi to toxic metals in soils (Zn and Cu). They concluded, that bacterial community is more sensitive to increased concentrations of heavy metals in soils than the fungal community. The relative fungal/bacterial ratio increased with increasing metal levels. Those authors also noticed the varying effect of soil pH on the microbial reaction to soil pollution, i.e. that lower pH in contaminated soils enhanced the negative effect on bacteria, but not on fungi.
The toxic concentration of heavy metals may cause enzyme damage and consequently their inactivation, as the enzymes-associated metals can be displaced by toxic metals with similar structure [59]. Moreover, heavy metals alter the conformational structures of nucleic acids and proteins, and consequently form complexes with protein molecules which render them inactive. Those effects result in disruption of microbial cell membrane integrity or destruction of entire cell [62]. Heavy metals also form precipitates or chelates with essential metabolites [63].
Various metals may affect different microbial populations and the resulting impact may vary depending on the metal whose limit concentrations in soils were exceeded. For instance, the pollution of soils with copper affects microorganisms that take part in nitrification and mineralization of protein compounds [50]. Silver is one of the most toxic metals to heterotrophic bacteria. This effect is used for the production of antiseptic preparations. However, there are some silver-resistant bacteria, both in clinical and natural conditions. Some strains of Thiobacillus ferrooxidans are able to accumulate particularly large amounts of silver [50]. About 100 ppm of zinc in soils may inhibit nitrification processes and about 1000 ppm inhibits the majority of microbiological processes in soils [64]. Microorganisms play vital role in circulation and transformation of mercury compounds in the environment. Numerous bacteria and fungi show high tolerance (also acquired) to increased concentrations of mercury in soils. However, some microorganisms are sensitive to excess mercury, e.g. the concentration of <10 ppm Hg may have toxic effects on nitrifiers in soils [50]. Increased concentrations of lead in surface soil layers negatively affect soil microflora. Processes of organic matter decomposition, particularly cellulose, are inhibited as a result of decreased enzymatic activity of microorganisms. This results in soil degradation. Biosorption of lead by soil microorganisms reaches on average 0.2% of this metal, but in some cases it may reach even 40% of biomass and may be used for biological remediation [50]. Some studies indicate that long-term contamination of soils with heavy metals has adverse effects on soil microbial activity. For instance, Juwarkar et al. [65] while researching the remediation strategies for cadmium and lead contaminated soils, compared the numbers of the selected groups of microorganisms in natural and heavy metal spiked soils. The results that they obtained indicated that the examined microbial groups were much less abundant in contaminated soils than in natural ones [Table 3]. On the other hand, Lenart and Wolny-Koładka [66] recorded significantly variable numbers of the selected microbial groups while analyzing the uncontaminated and heavy metal contaminated soils of ArcelorMittal steelworks in Cracow. Similarly, their results indicated that except for fungi, the soil-dwelling microorganisms were much less abundant in heavy metal polluted soils than in uncontaminated soils (Table 4). Heavy metal contamination results in reduction of microbial biomass and even if they do not cause the reduction in their number – they reduce biodiversity or disturb the community structure [64].
\n Analyzed microorganisms [CFU×g-1]\n | \n\n Natural soil\n | \n\n Heavy metal contaminated soil\n | \n
Total number of bacteria | \n17×105 | \n58×104 | \n
Total number of fungi | \n26×103 | \n42×102 | \n
Actinomycetes | \n43×103 | \n18×101 | \n
Azotobacter spp. | \n23×103 | \n17×101 | \n
Rhizobium spp. | \n21×103 | \n16×102 | \n
Microbiological characteristics of natural and heavy metal spiked spoil samples in Nagpur (India) [65]
\n Analyzed microorganisms [CFU×g-1]\n | \n\n Uncontaminated soil\n | \n\n Heavy metal contaminated soil\n | \n
Total number of mesophilic bacteria | \n22.50×102 – 10.44×106 | \n0 – 13.15×105 | \n
Total number of fungi | \n84.00×101 – 21.03×103 | \n0 – 57.90×103 | \n
Actinomycetes | \n62 – 99.50×103 | \n0 – 20.26×103 | \n
Azotobacter spp. | \n0 – 28.90×102 | \n0 – 57.00×101 | \n
Ranges of the selected microbial groups in heavy metal contaminated and uncontaminated soils of ArcelorMittal steelworks in Cracow, Poland [66].
However, one of the reasons of decreasing biodiversity of microorganisms in heavy metal polluted soils is the selection for tolerant species or strains. Metal exposure may lead to the establishment of tolerant microbial populations, that are often represented by several Gram-positive genera such as Bacillus, Arthrobacter and Corynebacterium or Gram-negatives, e.g. Pseudomonas, Alcaligenes, Ralstonia or Burkholderia [68]. It was shown that the impact of heavy metals on the bacterial metabolism depends on the growth form. The resistance towards metals seems higher in consortia than in pure cultures [69]. A great number of heavy metal-resistant bacteria, such as e.g. Cupriavidus metallidurans possess efflux transporters that excrete toxic or overconcentrated metals outside the cell [70]. Efflux transporters have high substrate affinity and can therefore maintain low cytosolic concentration of metals [9]. Alternatively, microbial cells may prevent the intoxication by the release of metal-binding compounds into the extracellular surroundings. In that case, metals are chelated outside the cell and thus blocked from entering the cell through the membrane transporters that otherwise facilitate the influx [9]. Some fungal and bacterial species are able to keep metals outside their cells by the extracellularly active melanin [71]. It is a secondary metabolite that has strong cation chelating properties through the anionic function such as carboxyl and deprotonated hydroxyl groups [9]. A substantial number of soil microorganisms, such as widespread fungus Aspergillus niger, solubilize metals by the release of organic acids or by the immobilization of metals through excretion of different compounds, such as oxalates [72]. Some microorganisms possess the abilities to protect their cells by a cytosolic sequestration mechanisms. These mechanisms are activated once the metal enters the cell and cannot be excreted. In this case internal inclusion bodies, e.g. polyphosphate granules (volutin) bind large amounts of metal cations [73]. Investigation and understanding of microbial resistance mechanisms towards heavy metals are crucial for the potential application of microorganisms for remediation of polluted soils.
The overall objective of any soil remediation approach is to create a final solution that is protective both for human health and the environment [74]. For heavy metal-polluted soils, the physical and chemical form of the heavy metal contaminant in soil strongly influences the selection of the appropriate remediation treatment approach. Details on the physical characteristics of polluted soils, type and level of the pollution at the site must be known to enable accurate assessment of the problem severity and adjustment of remedial measures [52].
Remediation of heavy metal-polluted sites is very expensive and difficult, therefore the best method to protect the environment from contamination is to prevent it. Nevertheless, it is not always possible and once metals are introduced and pollute the soil, they will remain there. Unlike carbon-based organic pollutants, heavy metals cannot be degraded or eliminated completely, therefore the traditional treatments for heavy metal pollution of soils are complicated and cost-intensive.
There are several technologies for remediation of heavy metal-polluted soils. One of the classifications divides the methods into in situ and ex situ treatment technologies. In situ (in place) means that the polluted soil is treated in its original location, i.e. it remains at the site or in the subsurface. Such technologies remove the pollutant from soil without excavation or removal of the soil. In this case fixing agents are applied on the unexcavated soil. This technique’s advantages may include low invasiveness, simplicity and rapidity. Moreover, it is fairly inexpensive and generates relatively low amount of waste. However, it is only a temporary solution. This is due to the fact that when physicochemical properties of soil change, the pollutants may again become active. Moreover, the reclamation process is applied only to the surface layer of soil [75]. Ex situ means that the treated soil is removed or excavated from the site [52]. It is applied in areas where heavily polluted soil must be removed from its place of origin and its storage is associated with high ecological risk. Fast and easy applicability, relatively low costs of investment and operation are the advantages of this method. On the other hand, it is highly invasive to the environment, generates a significant amount of solid wastes, and it is necessary to control the stored waste permanently. Evanko and Dzombak [76] divide in situ remediation strategies into solidification/stabilization, vitrification, soil flushing, electrokinetic extraction and biological treatment. Ex situ treatment technologies are divided by these authors into: solidification/stabilization, soil washing, vitrification and pyrometallurgical separation. Another classification of remedial strategies divides the technologies under five categories of general approaches to remediation: isolation, immobilization, toxicity reduction, physical separation, and extraction. There are several physicochemical techniques that include excavation and burial of soil at a hazardous waste site, chemical processing of soil to immobilize metals, leaching by using acid solutions or appropriate leachants to desorb metals from soil followed by the return of clean soil to the site [77], precipitation or flocculation followed by sedimentation, ion exchange, reverse osmosis and microfiltration [78]. Nevertheless, physicochemical techniques for heavy metal remediation are generally costly and have side effects [37]. Therefore, continuous efforts have been made to develop techniques that are easy to use, sustainable and economically feasible.
Phytoremediation is one of the best techniques for treatment of heavy metal-polluted sites. It is an in situ strategy that uses vegetation and associated microbiota together with agronomic practices to aid in metal remediation [79]. It is based on the use of special type of plants to decontaminate soil by inactivating metals in the rhizosphere or translocating them in the aerial parts [56]. Some plants developed mechanisms to remove ions selectively from the soil to regulate the uptake and distribution of metals. Potentially useful phytoremediation technologies for heavy metal-polluted sites include phytoextraction, phytostabilization and rhizofiltration [75].
Phytoextraction uses hyperaccumulating plants to remove metals from soil by absorption into the roots and shoots of the plant. The aboveground shoots can be then harvested to remove metals from the site and subsequently stored as hazardous waste or employed for the recovery of metals. The ideal plant for phytoextraction should grow rapidly, produce high amount of biomass and be able to tolerate and accumulate high metal concentrations in shoots [80]. Hyperaccumulating plants belong to the families of Brassicaceae, Fabaceae, Euphorbiaceae, Asterraceae, Lamiaceae, and Scrophulariaceae [77]. Studies indicate that many Brassica species, such as B. juncea, B. napus or B. rapa exhibit enhanced accumulation of Zn and Cd [81] In comparison to conventional methods like e.g. soil excavation (ex situ remediation), phytoextraction is time consuming, but on the other hand it is cost-effective and less labor-intensive [9].
Phytostabilization is based on the use of plants to limit the mobility and bioavailability of metals in soil. Plants used in this method are characterized by high tolerance of metals in surrounding soils together with their low accumulation. Phytostabilization can be carried out through the process of sorption, precipitation, complexation, or metal valence reduction. This technique is useful for the removal of Pb, As, Cd, Cr, Cu, and Zn [82]. This process is advantageous because in this case disposal of hazardous material/biomass is not required, and it is very effective when rapid immobilization is needed to preserve soils or ground and surface waters [82].
Rhizofiltration (or phytofiltration) removes metals from contaminated soil via absorption, concentration and precipitation by plant roots. This technique is used to remove pollutants from groundwater and aqueous-waste streams rather than for the remediation of polluted soils [76]. Apart from the above described phytoremediation methods, some authors [83] include also phytovolatization and phytodegradation.
Phytovolatization involves the use of plants to volatilize pollutants from their foliage such as Se and Hg, while phytodegradation uses plants and associated microorganisms to degrade organic pollutants. Even though phytoremediation strategies are inexpensive, effective, environmentally friendly and can be implemented in situ, a substantial proportion of metal pollutants are unavailable for root uptake by field grown plants [84]. Therefore, methods of increasing phytoavailability of heavy metal pollutants in soil and their transport to plant roots are vital to the success of in situ phytoremediation. In this case it is useful to apply microbial populations that are able to affect trace metal mobility and availability to plants, through the release of chelators, acidification and redox changes [85]. It was proved that the presence of rhizosphere bacteria increases the available concentrations of various heavy metals to hyperaccumulative plants [80]. Microbial populations may be used not only for increasing metal bioavailability to plants, but also for the promotion of hyperaccumulative plant growth through N2 fixation, production of phytohormones and siderophores, and transformation of nutrients [26]. Figure 2 summarizes the mechanisms of plant-mediated remediation of contaminated soils.
Mechanisms of phytoremediation involved in purifying contaminated soils and physiological processes that occur in plants during phytoremediation.
Another approach for biological remediation of heavy metal-polluted soils includes the use of microorganisms to detoxify metals by valence transformation, extracellular chemical precipitation or volatilization etc. [56]. Bioleaching is the method that uses microorganisms to solubilize heavy metal pollutants either by direct bacterial processes, or as a result of interactions with metabolic products, or both [76]. It can be used in situ or ex situ to help to remove the pollutants from soils. This process is based on mobilization of metal cations from insoluble ores by biological oxidation and complexation. This process was adapted from mining industry for the use in soil remediation and a general term covering both bioleaching and biooxidation techniques could be “biomining”. This technique is mainly employed for copper, cobalt, nickel, zinc and uranium, which are extracted either from insoluble sulfides or (in the case of uranium) from oxides [86]. The classical bioleaching bacteria belong to the genus Acidithiobacillus (A. thiooxidans and A. ferrooxidans), Acidiphilium, Acidimicrobium, Ferromicrobium or Sulfobacillus [86].
Another solution for soil bioremediation using microorganisms is to apply microbially-mediated biochemical processes, such as oxidation/reduction or methylation reactions [87]. Often, biostimulation and bioaugmentation are the components of bioremediation strategies. Biostimulation is a form of in situ bioremediation which uses growth rate stimulation nutrients, electron donors or acceptors to encourage the growth of site-specific indigenous microorganisms capable of degrading environmental pollutants. Common electron donors and acceptors used in biostimulation include: acetate, sulfate, nitrate and ethanol [88]. Bioaugmentation is the introduction of specific competent microorganisms to the local microbial population in order to increase the metabolic capacities needed for remediation [89]. Biosorption is a physicochemical process that occurs naturally and allows to passively concentrate and bind contaminants onto the microbial cell structure [90]. Metal biosorption by living organisms is a complicated process that consists of two steps. In the first step, metal ions are adsorbed on the cell surface by interactions between metals and cell surface functional groups. Biosorption of metal ions occurs primarily on the outer surface of microbial cells and is the first step in the interactions between metals and microbial cell walls [4]. The cell wall consists of a variety of polysaccharides and proteins, and hence offers a number of active sites capable of binding metal ions [91]. Differences in the cell wall composition among various microbial groups, i.e. algae, bacteria, cyanobacteria and fungi, cause significant differences in the type and amount of metal ions binding to them [91]. Physical adsorption via electrostatic or van der Waals forces allow to retain metal ions on the outer surfaces of bacterial cells. In addition to physical adsorption, ion exchange and complexation are believed to be the dominant mechanisms involved in metal biosorption [4]. The first step, passive biosorption, is metabolism-independent and proceeds rapidly by any one or a combination of metal binding mechanisms. In the second step, due to active biosorption, metal ions penetrate the cell membrane and enter into the cells. This is, however, a slowly occurring process. Active mode is metabolism-dependent and related to metal transport and deposition [91]. There are several microbial genera and species capable of metal biosorption. Fungi were found to be efficient biosorbent organisms, as their cells are characterized by a high percentage of cell wall material, which shows excellent metal binding properties [92]. Aureobasidium pullulans, Cladosporium resinae, Aspergillus niger, Aspergillus versicolor or Rhizopus nigricans are the fungal species proved to be effective in heavy metal biosorption [91]. Numerous studies also identified several species of bacteria as efficient metal accumulating microorganisms. For instance, Bacillus spp. has been reported to have a high potential of metal sequestration and has been used in commercial biosorbent preparation [91]. Other bacterial species capable of metal transformation include, among others: Escherichia coli, Pseudomonas maltophilia, Shewanella putrefaciens, Pseudomonas aeruginosa, Enterobacter cloacae [4].
Mechanisms involved in biochemical interactions between bacteria and metal ions involve specific enzymes that catalyze the oxidation, reduction, methylation, dealkylation and precipitation reactions. Microorganisms transform a substantial number of metals and metalloids by reducing or oxidizing them directly to a lower or higher redox state. Additionally, indirect oxidation or reduction is an alternative for immobilization of toxic metals in the environment. Methylation is an important process involved in geochemical cycling of metals and the removal of metal pollutants from soils. Methylation processes derive the methyl group from methylocarbolamine (CH3B12) which is implicated in the methylation of multiple metals and metalloids, such as Pb, Sn, Pd, Pt, Au, Ti, As, Se and Te [93]. Methylation of Hg, Sn and Pb can be mediated by a range of microbes, including Clostridium spp., methanogens and sulfate-reducing bacteria under anaerobic conditions and principally by fungi (e.g. Penicillium spp. and Alternaria spp.) under aerobic conditions. Methyl groups are enzymatically transferred to metals and a given species may transform a number of different metals [94]. Methyl-metal compounds are generally highly volatile and available to plants [50]. Another mechanism that has the potential for the application in heavy metal-polluted sites is the production of siderophores by different microbial genera. Siderophores are the largest class of compounds that can bind and transport Fe. They are highly specific Fe(III) ligands and are excreted by a wide variety of fungi and bacteria to aid Fe assimilation [94].
Microorganisms play an important role in the environmental biogeochemical cycle of metals and their properties are of significant interest in the remediation of contaminated sites. The microbial ability to absorb and transform metals is a promising aspect in respect of solving the pollution problems [4]. The potential of numerous microbial metal transformations in treatment of environmental pollution may be employed and some processes are already in commercial operation. However, many processes are still at the laboratory scale and yet to be tested in a rigorous applied and/or commercial context [94]. Another interesting aspect of the microbial community is their ability to multiply even under undesirable environmental conditions. These microorganisms sometimes affect soil environment more quickly than abiotic processes can. Therefore, the structure of soil microbial populations may be useful as a highly sensitive bioindicator of soil disturbance and progress of remediation [95].
Facing the increasing heavy metal pollution severity accompanied by rising land prices the communities around the world need to struggle for available investment grounds. This is mostly the problem of big cities, especially those with limited opportunities for development due to geographical barriers such as seashores, mountain ranges or desert areas. In such situations the polluted industrial areas cannot be left unused for long time to recover naturally. This creates a need for the development of various remedial procedures adjusted to changing contamination level, environmental conditions, available time and funding. Thus, remedial measures need to be almost always modified in order to meet those criteria. This makes that the continuous effort should be made to increase the effectiveness, flexibility and decrease the cost and side effects of the procedures available today. Although a number of measures was developed to remove the even toxic level of contamination, there are many degenerated areas that still cannot be successfully treated now. Those cases involve sites where remediation would be too expensive, time consuming or even technically disputable with currently available treatment procedures.
Heavy metals pose a significant threat towards the soil environment and the rapid industrialization will result in increasing problems of environmental pollution. Therefore, it is necessary to carry out the continuous monitoring of both industrial areas and their vicinities for possible transgressions of the limits given by the authorities. When necessary, the remedial measures should be applied as soon as possible by all available means. On the other hand, research should be promoted to understand the mechanisms of microbial response to heavy metal pollution and to enable screening for possible resistant microorganisms that could be used for both remediation and restoration of soil environment fertility.
Erythrocytes are the major cellular component of the circulating blood. Roughly, erythrocytes in circulation average about 5 million cells per cubic millimetres of blood. With an average life span of about 100–120 days, erythrocyte production and senescence is maintained in constant equilibrium. Any imbalances affecting production or destruction of red cells result in red cell disorder. In essence, red cells are maintained at a constant volume in the body, depending on several factors. Physiologic factors such as age, sex, altitude, smoking status or pregnancy account for slight inter-individual and intra-individual variations. Typically, there are different measures of red cell counts and they include red cell mass, red cell volume, red cell count, haematocrit and haemoglobin concentration. Red cell volume or mass is expected to fall within an interval of mean ± 2 SD within a specified population for a person’s age, sex and race.
\nBeyond count anomalies (quantitative abnormalities), morphologic aberrations (qualitative abnormalities) are highly relevant in clinical evaluation of red cell diseases. Normally, a red cell has a round form, shaped like a disc, well-haemoglobinised cytoplasmic rim with a central pallor covering inner third of the red cell. Deviations in morphology (size, shape, colour, contents/inclusion or distribution) may be associated or perhaps diagnostic of disease entities. For instance, a blood picture with paucity of red cells, numerous red cell fragments, increased polychromatic red cells suggests a micro-angiopathy or fragmentation syndrome.
\nThis chapter aims to discuss principles of red cell morphology, as well as describe red cells in terms of morphology, identify morphologic abnormalities associated with different disease conditions.
\nCirculating red cells are formed from bone marrow stem cells. Stem cells are pluripotent; they self-replicate and differentiate to specialized cells in circulation through different lineages. Red cells are formed from the myeloid stem cell lineage (colony forming unit—granulocytes, erythroid, myeloid and megakaryocytes). The earliest recognizable red cell precursor in the bone marrow is the pronormoblast. The pronormoblast undergoes series of maturation to become the orthochromatic normoblast. Upon extrusion of its nucleus, the late normoblast becomes the shift reticulocytes, which is released into the circulation. Finally, DNA remnants and other chromatin materials in the reticulocytes is removed by the pitting action of the spleen, hence the mature red cells.
\nErythrocytes cannot be seen with the naked eyes. Typically, morphology of red cells is performed on peripheral blood smears, once there is an indication. Erythrocyte morphology is either indicated by a clinical request or laboratory flags. Examples of clinical indications for peripheral blood film/erythrocyte morphology are listed in Table 1.
\nA clinical request for a PBF may be prompted by the following indications: | \n
\n
| \n
\n
| \n
\n
| \n
\n
| \n
\n
| \n
\n
| \n
\n
| \n
\n
| \n
\n
| \n
\n
| \n
\n
| \n
\n
| \n
\n
| \n
Clinical indications for peripheral blood film.
Erythrocyte morphology may also be indicated when significant deviations from the normal are seen in the laboratory during blood work (full blood count) irrespective of a clinical request. For instance, a significantly reduced haemoglobin level with low MCV and raised RDW may suggest iron deficiency anaemia. This is an indication for red cell morphology and other ancillary investigation for iron deficiency.
\nBlood for peripheral blood film is collected through venipuncture. Anticoagulant of choice is the potassium EDTA. Specimens should be analysed as soon as possible, preferably within 2 hours of blood collection. Samples not analysed immediately should be stored at 2–6°C in a refrigerator, or the blood smear should be made, dried and fixed, for subsequent staining with Romanowsky dyes.
\nAsides automated slide makers, the commonest method for preparation of peripheral blood film is the slide ‘wedge’ or push technique. This technique typically requires microscope slides, pipette/blood dropper, spreader slide and the blood specimen to be analysed. Standard precautions must be observed to prevent transmission of infectious pathogens such as human immunodeficiency virus and hepatitis viruses.
\nQuality control measures will include ensuring proper anticoagulant: blood ratio, sample processing/analysis within sample viability period and adequate mixing of the blood before smearing. Each slide must be labelled with at least two patient identifiers such as name and laboratory, and date of procedure. Once the smear is air-dried in about 5–10 minutes, fixation of the blood tissue is another very important step. Fixation helps to preserve the architecture of the cells, which ensures good morphology. A dried slide should be fixed within 4 hours of preparation, preferably in the first hour.
\nFor routine morphology, the glass slides are stained with Romanowsky dyes. Romanowsky dyes are differential stains composed of both acidic and basic components. The acidic component is eosin and the basic part is azure B or polychrome methylene blue. Examples of Romanowsky stains include Leishman stain, Jenner, Wright stain, May-Grunwald-Giemsa stain and Giemsa stain. Generally, the eosin part of the dye binds to the basic component of the cell such as the haemoglobin molecules in the red cell and stains it pink. The basophilic part of the dye binds to the acidic component of the cells such as the nucleus and stains it blue. Other components of the cells appear in different colour shades that contrasts and compares with the dye. The term, azurophilic is used to describe a neutral to sky-blue colour shade. For instance, the cytoplasm of a neutrophil is described as azurophilic in colour. Furthermore, the characteristic staining quality of different red cell components is presented in Table 2.
\nCell component | \nColour | \n
---|---|
Chromatin (including H-J bodies) | \nPurple | \n
Cytoplasm with RNA and nuclear Remnants (e.g. polychromasia, basophilic stippling’s) | \nIn polychromatic red cells, RNA produces a blue colour, which offsets the pink colour imparting a purple tinge Basophilic stippling: appears as blue granules dispersed within the cytoplasm in a punctate appearance | \n
Mature red cells | \nPink | \n
Romanowsky staining characteristics of red cell components.
Staining procedure and the stain contact time depends on the type of dye in use. Staining protocols are contained in standard laboratory texts and reagent manuals. Red cell morphology should be examined at the monolayer region of the film which is 2–4 × 10 fields from the feathered edge. In this place red cells are randomly distributed with most lying singly and only a few overlapping. If area is too thin, the RBCs will appear flat with no central pallor. If too thick, false rouleaux may be reported and morphology may be difficult to evaluate because red cells are packed.
\nThe haemato-morphologist reviews the red cell morphology under the compound microscope and notes any significant abnormalities for reporting/diagnosis in light of patient clinical context. Red cell morphology is evaluated in terms of size, shape, colour, distribution and intra cytoplasmic inclusions. In general, red cells have a fairly uniform variation in size, with a red cell distribution width of 11–15% in normal individuals. Abnormal variations in sizes and shape are termed anisocytosis and poikilocytosis, respectively [1].
\nNormal red cells (normocytes) are about 7–8 μm in diameter [2]. Reduced size is termed microcytosis. Increase in red cell diameter above normal is called macrocytosis. Red cell sizes form the basis for morphologic or cytometric classification of anaemia. In terms of red cell size, anaemia could be described as microcytic, normocytic or macrocytic. Typically, the normal red cell size is adjudged by comparison with the nucleus of a small lymphocyte. The reference interval for mean red cell volume (MCV) is 80–95 fl [3, 4]. MCV >95 fl is termed macrocytic. While, red cell size <6 μm and/or MCV <80 fl is termed microcytic [5]. Differentials of microcytic anaemias include iron deficiency, thalassemias, sideroblastic anaemia and anaemia of chronic inflammation (20% of cases). Further test such as serum ferritin, total iron binding capacity (TIBC), haemoglobin electrophoresis with quantification helps to differentiate microcytic anaemia [4, 6]. For instance, low serum ferritin, raised TIBC and raised RDW is expected in iron deficiency. A normal or elevated red cell counts with little red cell size variation (RDW) in the presence of microcytosis is suggestive of a thalassaemia.
\nNormocytic anaemia occurs in acute blood loss, marrow aplasia, anaemia of chronic disease (80% of cases) and anaemias of endocrine origin. Macrocytosis may be oval or round, with specific casual relationships. Oval macrocytes are seen in megaloblastic anaemias (folate/cobalamin deficiencies), myelodysplastic syndrome and drug therapies such as hydroxyurea [7]. Round macrocytes are seen in liver disease and excess alcohol use. MCV may appear falsely normal with the haematology analyser in combined substrate deficiency states. However, the blood picture will reveal marked anisopoikilocytosis. The red cell distribution width (RDW) is a calculated parameter and it measures the individual size variability (heterogeneity) of the red cells. RDW is the percentage coefficient of variation of the individual red cell volumes enumerated by the particle counter [8]. RDW normally ranges between 11.5 and 15.5%. For interpretation purposes, raised RDW is seen in iron deficiency anaemia, megaloblastic anaemia (folate and cobalamin deficiency), haemolytic anaemia, recent blood transfusion, hereditary spherocytosis and sickle cell syndromes [8, 9]. RDW is useful in interpreting apparently normal MCV since it will be quite high in combined micronutrient deficiency state.
\nShape abnormalities, otherwise called poikilocytes are useful pointers to specific diagnosis. It is important to note that poikilocytosis may also occur in vitro (artefactual causes). It is therefore necessary to ensure adequate precautions in reducing pre-analytic and intra-analytic errors that affects morphology. As a reminder, the following quality control measures apply in blood film morphology:
Blood specimens for PBF are best collected in EDTA bottles through venipuncture.
Optimal blood: anticoagulant ratio should be observed.
Samples should be dispatched immediately to the haematology laboratory. Prolonged delay in analysis allows for cellular degeneration, pseudo-thrombocytopenia and artefactual changes [10].
Blood specimens for morphology are best analysed within 2 hours of collection.
Poikilocytes are categorized as either spiculated or non-spiculated. Spiculated red cells have at least one pointed projection from the cell surface. Examples of spiculated poikilocytes are burr cells, schistocytes (red cell fragments), irreversibly sickled red cells (drepanocytes), acanthocytes and tear drop red cells (dacrocytes). Non-spiculated poikilocytes include target cells, ovalocytes and stomatocytes. Various mechanical, biochemical and molecular mechanisms underlie pathologic changes in red cell shape. Some occur as a result of disturbances in the haematopoietic system. Target cells have an area of central haemoglobinization (termed hyperchromic bull eyes) surrounded by a halo of pallor. Increased red cell surface area to volume ratio in target cells is due to its redundant membrane, which gives rise to the targetoid shape. Target cells (Figure 1) are seen in sickle haemoglobinopathies, thalassemias, iron deficiency and post splenectomy state. Tear drop red cells (Figure 2) results from abnormal spleen or bone marrow pathology such as primary myelofibrosis when the red cells stretch out in order to navigate its way into the periphery or as a result of stretching from the pitting action of the spleen, when red cells with inclusions such as Heinz bodies navigates the splenic cords into the sinuses [5].
\n(1) Nucleated red cell, (2) target cell, and (3) irreversibly sickled red cell.
(4) Tear drop red cell.
Stomatocytes have a fish mouth appearance (slit-like central pallor). They are mostly due to increased red cell permeability, resulting in increased volume. Stomatocytes may be inherited or acquired. Hereditary stomatocytosis is seen in Rh null phenotype. Acquired stomatocytosis is mostly seen with recent excessive alcohol and typically resolves within 2 weeks of alcohol withdrawal. When artefactual, stomatocytes are usually <10% of the red cell population. As the name implies, irreversibly sickled red cells (Figure 1) are seen in sickle syndromes. The primary event is intra-erythrocytic haemoglobin precipitation (gelation), with resultant formation of tactoids, which deforms the discoid red cell to sickle or crescent morphology [11]. Burr cells are seen in renal failure and may be artefactual. Artefactual red cells may be caused by poor fixation and high humidity in the laboratory ambience. Artefactual tear drop cells should be suspected if the tails line up in the same direction. Table 3 itemizes common poikilocytes and its differentials [1, 5, 12, 13, 14, 15].
\nRed cell shapes | \nDifferential diagnosis | \n
---|---|
Irreversibly sickled red cells (drepanocytes) | \nSickle cell syndromes (SS, SC, S-β-thalassemia) | \n
Target red cells (codocytes) Target cells (codocytes, Mexican hat cells) | \nSickle cell disease, haemoglobin C trait, haemoglobin CC disease, thalassemia’s, iron deficiency, liver disease (cholestasis), asplenia | \n
Fragmented red cells (schistocytes, helmet cells, keratocytes) | \nThrombotic micro-angiopathic haemolytic anaemias such as disseminated intravascular coagulopathy (DIC), thrombotic thrombocytopenic purpura, haemolytic uraemic syndrome. | \n
Pencil cells | \nIron deficiency | \n
Stomatocytes | \nArtefact (due to slow drying in humid environment), liver disease, alcoholism, Rh-null disease, obstructive lung disease | \n
Elliptocytes | \nHereditary elliptocytosis (>25%) | \n
Bite cells (degmacytes) | \nG6PD deficiency, oxidative stress, unstable haemoglobin’s, congenital Heinz body anaemia | \n
Basket cells (half ghost cells/blister cells) | \nOxidant damage, G6PD deficiency, unstable haemoglobin’s | \n
Spherocytes | \nHereditary spherocytosis, ABO incompatibility, autoimmune haemolytic anaemia (warm antibody type), severe burns | \n
Teardrop red cell (dacrocytes, lacrymocytes) | \nIdiopathic myelofibrosis, myelophthisic anaemia, thalassemia’s | \n
Red cell shape anomalies and associated diseases.
Anisochromia depicts increased or decreased haemoglobinization of the red cells. In hypochromic red cells, the central pallor exceeds one third of the diameter. Hypochromia usually follows microcytosis, as seen in iron deficiency states. Hyperchromia (increased haemoglobinisation) is associated with shape abnormalities such as (micro)-spherocytes and sickled red cells. Increased haemoglobinization obliterates central pallor. Occasionally, severe hypochromia is associated with macrocytic red cells, termed leptocytes. Leptocytes are seen in severe iron deficiency, thalassemia and liver diseases [14]. Polychromasia on PBF suggests in-vivo reticulocytosis. Literally, polychromasia means ‘many colours’, i.e. the red cells bear another shade of colour than pink (eosinophilic). Polychromatic red cells are macrocytic (young red cells) and have a bluish tinge. The blue tinge denotes the presence of rRNA which eventually undergo the pitting action of the spleen to become mature circulating red cells [1]. Normally, polychromatic red cells are not obvious on PBF—adult reticulocyte population is about 0.5–2.5% [3]. However, polychromatic red cells in excess of 1–2% in the periphery should be considered significant since normal daily rate of red cell turnover is about 1–2% [16]. In situations of acute haemorrhage, haemolysis, and high altitude, hypoxia induces increased erythroid activity, hence polychromasia. Polychromasia is also seen in extramedullary haemopoiesis due to myeloid metaplasia in reticulo-endothelial tissue. Following haematinic therapy, polychromatic red cells are seen as a response to treatment of micronutrient deficiency [1].
\nSimilarly, in severe situations causing marrow stress, nucleated red cells (erythroblastosis) exit the bone marrow prematurely in order to compensate. Notable causes of erythroblastosis (or normoblastemia) include severe anaemia, asplenic/hyposplenic state as in sickle cell disease, severe hypoxia, marrow replacements or infiltrations and extramedullary haemopoiesis [17, 18]. In neonates, nucleated red cells are normally seen in the periphery [15].
\nOther morphologic abnormalities include presence of inclusion bodies and pathologic distribution of red cells on the smear. A mature erythrocyte lacks inclusion bodies. Red cell inclusion bodies include nuclear products RNA/DNA, haemoglobin or iron pigments. Some, such as haemoglobin H inclusions and Heinz bodies can only be appreciated with supravital staining. Red cell inclusions result from oxidant stress, severe infections and dyserythropoiesis (maturation defects). Basophilic stipplings or punctuate basophilia are denatured RNA fragments dispersed within the cytoplasm. Basophilic stipplings may be fine, blue stipplings or coarse granules. They are non-specific and are generally related to disorders in haem biosynthetic pathways [1, 19]. Differentials include haemoglobinopathies (thalassemias), lead or arsenic poisoning, unstable haemoglobins, severe infections, sideroblastic anaemia, megaloblastic anaemia and a rare inherited condition, pyrimidine 5′ nucleotidase deficiency [1, 10, 20].
\nClinically insignificant, fine basophilic stippling may be associated with polychromasia/accelerated erythropoiesis/reticulocytosis. Coarse stipplings are clinically significant and indicates impaired haemoglobin synthesis as seen in megaloblastic anaemia, thalassemias, sideroblastic anaemias and lead poisoning [1, 19]. Unlike other basophilic inclusions such as Howell jolly bodies and Pappenheimer bodies which tend to be displaced to the periphery, basophilic stipplings are diffusely dispersed throughout the red cell cytoplasm. Howell jolly bodies (Figure 3) are DNA remnants seen in post-splenectomy patients, anatomical or functional asplenia. Siderotic granules or Pappenheimer bodies appear purple on Romanowsky stain, blue on Perl’s stain and are seen in disorders of iron utilization like sideroblastic anaemias.
\n(5) Howell Jolly body (in a 36-year-old lady with sickle cell disease).
Parasites such as Plasmodium spp. or Babesia spp. may also be seen on peripheral blood smear [21]. Both parasites invade the red cells. Their identification requires some level of knowledge and experience. Several species of Plasmodium spp. exist. Plasmodium spp. may exist in different forms such as ring forms (trophozoites), gametocytes and schizonts. Babesia spp. appear in small ring forms (like Plasmodium falciparum) but schizonts and gametocytes are not formed [1, 21]. Unlike Plasmodium spp., Babesia spp. do not produce pigments. However, Babesia spp. may appear in groups outside the erythrocyte. Clinical history and travel history is also helpful in differentiating the two parasites. Other red cell inclusions such as Heinz bodies and haemoglobin H inclusions can only be appreciated with supravital staining (reticulocyte preparations). Heinz bodies are denatured haemoglobin (seen in oxidant injury, G6PD deficiency). Haemoglobin H inclusions are seen in alpha-thalassemias giving rise to the characteristic ‘golf ball’ appearance of the erythrocytes [1, 11, 12].
\nRouleaux formation refers to stacking of red cells like coins in a single file. Rouleaux is seen in hyperproteinaemias. Elevated plasma fibrinogen or globulins reduces the zeta potential (repulsive force) between circulating red cells, facilitating their stacking effect. Rouleaux is associated with myeloma/paraproteinaemias, other plasma cell disorders as well as B cell lymphomas. On the other hand, agglutination refers to clumping or aggregation of red cells into clusters or masses and is usually antibody mediated [1]. Agglutination of red cells may be seen in cold haemagglutinin disease and Waldenstrom’s macroglobulinaemia [1, 11]. Agglutination is associated with falsely reduced red cell count and high MCV. Pre warming the specimen with heating block helps to disperse the red cells prior to making of a blood smear and automated cell counts.
\nRed cell morphology is crucial in evaluating anaemias and several blood disorders. Good quality smear, with proper Romanowsky/special staining, coupled with the expertise of an haemato-morphologists (haematologists/haematology pathologists) remains highly valuable in patient care.
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",metaTitle:"Waiver Policy",metaDescription:"We feel that financial barriers should never prevent researchers from publishing their research. With the need to make scientific research more publically available and support the benefits of Open Access, more institutions and funders have dedicated funds to assist their faculty members and researchers cover the APCs associated with publishing in Open Access. Below we have outlined several options available to secure financing for your Open Access publication.",metaKeywords:null,canonicalURL:"/page/waiver-policy",contentRaw:'[{"type":"htmlEditorComponent","content":"At IntechOpen, the majority of OAPFs are paid by an Author’s institution or funding agency - Institutions (73%) vs. Authors (23%).
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\\n"}]'},components:[{type:"htmlEditorComponent",content:'At IntechOpen, the majority of OAPFs are paid by an Author’s institution or funding agency - Institutions (73%) vs. Authors (23%).
\n\nThe first step in obtaining funds for your Open Access publication begins with your institution or library. IntechOpen’s publishing standards align with most institutional funding programs. Our advice is to petition your institution for help in financing your Open Access publication.
\n\nHowever, as Open Access becomes a more commonly used publishing option for the dissemination of scientific and scholarly content, in addition to institutions, there are a growing number of funders who allow the use of grants for covering OA publication costs, or have established separate funds for the same purpose.
\n\nPlease consult our Open Access Funding page to explore some of these funding opportunities and learn more about how you could finance your IntechOpen publication. Keep in mind that this list is not definitive, and while we are constantly updating and informing our Authors of new funding opportunities, we recommend that you always check with your institution first.
\n\nFor Authors who are unable to obtain funding from their institution or research funding bodies and still need help in covering publication costs, IntechOpen offers the possibility of applying for a Waiver.
\n\nOur mission is to support Authors in publishing their research and making an impact within the scientific community. Currently, 14% of Authors receive full waivers and 6% receive partial waivers.
\n\nWhile providing support and advice to all our international Authors, waiver priority will be given to those Authors who reside in countries that are classified by the World Bank as low-income economies. In this way, we can help ensure that the scientific work being carried out can make an impact within the worldwide scientific community, no matter where an Author might live.
\n\nThe application process is open after your submitted manuscript has been accepted for publication. To apply, please fill out a Waiver Request Form and send it to your Author Service Manager. If you have an official letter from your university or institution showing that funds for your OA publication are unavailable, please attach that as well. The Waiver Request will normally be addressed within one week from the application date. All chapters that receive waivers or partial waivers will be designated as such online.
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I am also a member of the team in charge for the supervision of Ph.D. students in the fields of development of silicon based planar waveguide sensor devices, study of inelastic electron tunnelling in planar tunnelling nanostructures for sensing applications and development of organotellurium(IV) compounds for semiconductor applications. I am a specialist in data analysis techniques and nanosurface structure. 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After obtaining a Master's degree in Mechanical Engineering, he continued his PhD studies in Robotics at the Vienna University of Technology. Here he worked as a robotic researcher with the university's Intelligent Manufacturing Systems Group as well as a guest researcher at various European universities, including the Swiss Federal Institute of Technology Lausanne (EPFL). During this time he published more than 20 scientific papers, gave presentations, served as a reviewer for major robotic journals and conferences and most importantly he co-founded and built the International Journal of Advanced Robotic Systems- world's first Open Access journal in the field of robotics. Starting this journal was a pivotal point in his career, since it was a pathway to founding IntechOpen - Open Access publisher focused on addressing academic researchers needs. Alex is a personification of IntechOpen key values being trusted, open and entrepreneurial. Today his focus is on defining the growth and development strategy for the company.",institutionString:null,institution:{name:"TU Wien",country:{name:"Austria"}}},{id:"19816",title:"Prof.",name:"Alexander",middleName:null,surname:"Kokorin",slug:"alexander-kokorin",fullName:"Alexander Kokorin",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/19816/images/1607_n.jpg",biography:"Alexander I. Kokorin: born: 1947, Moscow; DSc., PhD; Principal Research Fellow (Research Professor) of Department of Kinetics and Catalysis, N. Semenov Institute of Chemical Physics, Russian Academy of Sciences, Moscow.\r\nArea of research interests: physical chemistry of complex-organized molecular and nanosized systems, including polymer-metal complexes; the surface of doped oxide semiconductors. He is an expert in structural, absorptive, catalytic and photocatalytic properties, in structural organization and dynamic features of ionic liquids, in magnetic interactions between paramagnetic centers. The author or co-author of 3 books, over 200 articles and reviews in scientific journals and books. He is an actual member of the International EPR/ESR Society, European Society on Quantum Solar Energy Conversion, Moscow House of Scientists, of the Board of Moscow Physical Society.",institutionString:null,institution:{name:"Semenov Institute of Chemical Physics",country:{name:"Russia"}}},{id:"62389",title:"PhD.",name:"Ali Demir",middleName:null,surname:"Sezer",slug:"ali-demir-sezer",fullName:"Ali Demir Sezer",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/62389/images/3413_n.jpg",biography:"Dr. Ali Demir Sezer has a Ph.D. from Pharmaceutical Biotechnology at the Faculty of Pharmacy, University of Marmara (Turkey). 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Focus of his research activity is drug delivery, physico-chemical characterization and biological evaluation of biopolymers micro and nanoparticles as modified drug delivery system, and colloidal drug carriers (liposomes, nanoparticles etc.).",institutionString:null,institution:{name:"Marmara University",country:{name:"Turkey"}}},{id:"61051",title:"Prof.",name:"Andrea",middleName:null,surname:"Natale",slug:"andrea-natale",fullName:"Andrea Natale",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:null},{id:"100762",title:"Prof.",name:"Andrea",middleName:null,surname:"Natale",slug:"andrea-natale",fullName:"Andrea Natale",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"St David's Medical Center",country:{name:"United States of America"}}},{id:"107416",title:"Dr.",name:"Andrea",middleName:null,surname:"Natale",slug:"andrea-natale",fullName:"Andrea Natale",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Texas Cardiac Arrhythmia",country:{name:"United States of America"}}},{id:"64434",title:"Dr.",name:"Angkoon",middleName:null,surname:"Phinyomark",slug:"angkoon-phinyomark",fullName:"Angkoon Phinyomark",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/64434/images/2619_n.jpg",biography:"My name is Angkoon Phinyomark. I received a B.Eng. degree in Computer Engineering with First Class Honors in 2008 from Prince of Songkla University, Songkhla, Thailand, where I received a Ph.D. degree in Electrical Engineering. My research interests are primarily in the area of biomedical signal processing and classification notably EMG (electromyography signal), EOG (electrooculography signal), and EEG (electroencephalography signal), image analysis notably breast cancer analysis and optical coherence tomography, and rehabilitation engineering. I became a student member of IEEE in 2008. During October 2011-March 2012, I had worked at School of Computer Science and Electronic Engineering, University of Essex, Colchester, Essex, United Kingdom. In addition, during a B.Eng. I had been a visiting research student at Faculty of Computer Science, University of Murcia, Murcia, Spain for three months.\n\nI have published over 40 papers during 5 years in refereed journals, books, and conference proceedings in the areas of electro-physiological signals processing and classification, notably EMG and EOG signals, fractal analysis, wavelet analysis, texture analysis, feature extraction and machine learning algorithms, and assistive and rehabilitative devices. I have several computer programming language certificates, i.e. Sun Certified Programmer for the Java 2 Platform 1.4 (SCJP), Microsoft Certified Professional Developer, Web Developer (MCPD), Microsoft Certified Technology Specialist, .NET Framework 2.0 Web (MCTS). 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