\r\n\tThis book intends to provide the reader with a comprehensive overview of the current state-of-the-art novel imaging techniques by focusing on the most important evidence-based developments in this area.
",isbn:null,printIsbn:null,pdfIsbn:null,doi:null,price:0,priceEur:0,priceUsd:0,slug:null,numberOfPages:0,isOpenForSubmission:!0,isSalesforceBook:!1,isNomenclature:!1,hash:"d9159ce31733bf78cc2a79b18c225994",bookSignature:"Dr. Gabriel Cismaru",publishedDate:null,coverURL:"https://cdn.intechopen.com/books/images_new/11867.jpg",keywords:"Hypertrophic Cardiomyopathy, Dilated Cardiomyopathy, Restrictive Cardiomyopathy, Transesophageal Echocardiography, Intracardiac Echocardiography, 3-Dimensional Echocardiography, Adult Congenital Heart Disease, Tetralogy of Fallot, Transposition of the Great Vessels, Coronary Artery Disease, Risk Stratification, Revascularization",numberOfDownloads:null,numberOfWosCitations:0,numberOfCrossrefCitations:null,numberOfDimensionsCitations:null,numberOfTotalCitations:null,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"April 21st 2022",dateEndSecondStepPublish:"May 19th 2022",dateEndThirdStepPublish:"July 18th 2022",dateEndFourthStepPublish:"October 6th 2022",dateEndFifthStepPublish:"December 5th 2022",dateConfirmationOfParticipation:null,remainingDaysToSecondStep:"3 months",secondStepPassed:!0,areRegistrationsClosed:!0,currentStepOfPublishingProcess:4,editedByType:null,kuFlag:!1,biosketch:"Dr. Cismaru Gabriel is an Assistant Professor at the University of Medicine and Pharmacy Cluj-Napoca, certified in Cardiology. After completing his certification in cardiology, Dr. Cismaru began his electrophysiology fellowship at the Institut Lorrain du Coeur et des Vaisseaux Louis Mathieu. He has authored or co-authored peer-reviewed articles and book chapters in the field of cardiac pacing, defibrillation, electrophysiological study, and catheter ablation.",coeditorOneBiosketch:"Raluca Tomoaia is an MD, Ph.D. in novel techniques in Echocardiography at the University of Medicine and Pharmacy in Cluj-Napoca, Romania., assistant professor, and a researcher in echocardiography and cardiovascular imaging.",coeditorTwoBiosketch:null,coeditorThreeBiosketch:null,coeditorFourBiosketch:null,coeditorFiveBiosketch:null,editors:[{id:"191888",title:"Dr.",name:"Gabriel",middleName:null,surname:"Cismaru",slug:"gabriel-cismaru",fullName:"Gabriel Cismaru",profilePictureURL:"https://mts.intechopen.com/storage/users/191888/images/system/191888.png",biography:"Dr. Cismaru Gabriel is an assistant professor at the Cluj-Napoca University of Medicine and Pharmacy, Romania, where he has been qualified in cardiology since 2011. He obtained his Ph.D. in medicine with a research thesis on electrophysiology and pro-arrhythmic drugs in 2016. Dr. Cismaru began his electrophysiology fellowship at the Institut Lorrain du Coeur et des Vaisseaux Louis Mathieu, France, after finishing his cardiology certification with stages in Clermont-Ferrand and Dinan, France. He began working at the Rehabilitation Hospital\\'s Electrophysiology Laboratory in Cluj-Napoca in 2011. He is an experienced operator who can implant pacemakers, CRTs, and ICDs, as well as perform catheter ablation of supraventricular and ventricular arrhythmias such as ventricular tachycardia and ventricular fibrillation. He has been qualified in pediatric cardiology since 2022, and he regularly performs device implantation and catheter ablation in children. Dr. Cismaru has authored or co-authored peer-reviewed publications and book chapters on cardiac pacing, defibrillation, electrophysiological studies, and catheter ablation.",institutionString:"Iuliu Hațieganu University of Medicine and Pharmacy",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"7",totalChapterViews:"0",totalEditedBooks:"2",institution:{name:"Iuliu Hațieganu University of Medicine and Pharmacy",institutionURL:null,country:{name:"Romania"}}}],coeditorOne:null,coeditorTwo:null,coeditorThree:null,coeditorFour:null,coeditorFive:null,topics:[{id:"16",title:"Medicine",slug:"medicine"}],chapters:null,productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"},personalPublishingAssistant:null},relatedBooks:[{type:"book",id:"5970",title:"Bedside Procedures",subtitle:null,isOpenForSubmission:!1,hash:"ba56d3036ac823a7155f40e4a02c030d",slug:"bedside-procedures",bookSignature:"Gabriel Cismaru",coverURL:"https://cdn.intechopen.com/books/images_new/5970.jpg",editedByType:"Edited by",editors:[{id:"191888",title:"Dr.",name:"Gabriel",surname:"Cismaru",slug:"gabriel-cismaru",fullName:"Gabriel Cismaru"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"9064",title:"Epidemiology and Treatment of Atrial Fibrillation",subtitle:null,isOpenForSubmission:!1,hash:"1cd6bf2b3181eb82446347fbe478a2bc",slug:"epidemiology-and-treatment-of-atrial-fibrillation",bookSignature:"Gabriel Cismaru and Keith Andrew Chan",coverURL:"https://cdn.intechopen.com/books/images_new/9064.jpg",editedByType:"Edited by",editors:[{id:"191888",title:"Dr.",name:"Gabriel",surname:"Cismaru",slug:"gabriel-cismaru",fullName:"Gabriel Cismaru"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"6550",title:"Cohort Studies in Health Sciences",subtitle:null,isOpenForSubmission:!1,hash:"01df5aba4fff1a84b37a2fdafa809660",slug:"cohort-studies-in-health-sciences",bookSignature:"R. 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Venkateswarlu",coverURL:"https://cdn.intechopen.com/books/images_new/371.jpg",editedByType:"Edited by",editors:[{id:"58592",title:"Dr.",name:"Arun",surname:"Shanker",slug:"arun-shanker",fullName:"Arun Shanker"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"3092",title:"Anopheles mosquitoes",subtitle:"New insights into malaria vectors",isOpenForSubmission:!1,hash:"c9e622485316d5e296288bf24d2b0d64",slug:"anopheles-mosquitoes-new-insights-into-malaria-vectors",bookSignature:"Sylvie Manguin",coverURL:"https://cdn.intechopen.com/books/images_new/3092.jpg",editedByType:"Edited by",editors:[{id:"50017",title:"Prof.",name:"Sylvie",surname:"Manguin",slug:"sylvie-manguin",fullName:"Sylvie Manguin"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"72",title:"Ionic Liquids",subtitle:"Theory, Properties, New Approaches",isOpenForSubmission:!1,hash:"d94ffa3cfa10505e3b1d676d46fcd3f5",slug:"ionic-liquids-theory-properties-new-approaches",bookSignature:"Alexander Kokorin",coverURL:"https://cdn.intechopen.com/books/images_new/72.jpg",editedByType:"Edited by",editors:[{id:"19816",title:"Prof.",name:"Alexander",surname:"Kokorin",slug:"alexander-kokorin",fullName:"Alexander Kokorin"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"2270",title:"Fourier Transform",subtitle:"Materials Analysis",isOpenForSubmission:!1,hash:"5e094b066da527193e878e160b4772af",slug:"fourier-transform-materials-analysis",bookSignature:"Salih Mohammed Salih",coverURL:"https://cdn.intechopen.com/books/images_new/2270.jpg",editedByType:"Edited by",editors:[{id:"111691",title:"Dr.Ing.",name:"Salih",surname:"Salih",slug:"salih-salih",fullName:"Salih Salih"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}}]},chapter:{item:{type:"chapter",id:"45095",title:"Biodegradable Polymers",doi:"10.5772/56230",slug:"biodegradable-polymers",body:'\nIn developing countries, environmental pollution by synthetic polymers has assumed dangerous proportions. Petroleum-derived plastics are not readily biodegradable and because of their resistance to microbial degradation, they accumulate in the environment. In addition in recent times oil prices have increased markedly. These facts have helped to stimulate interest in biodegradable polymers. Biodegradable plastics and polymers were first introduced in 1980s. Polymers from renewable resources have attracted an increasing amount of attention over the last two decades, predominantly due to two major reasons: firstly environmental concerns, and secondly the realization that our petroleum resources are finite. There are many sources of biodegradable plastics, from synthetic to natural polymers. Natural polymers are available in large quantities from renewable sources, while synthetic polymers are produced from non-renewable petroleum resources. Biodegradation of polymeric biomaterials involves cleavage of hydrolytically or enzymatically sensitive bonds in the polymer leading to polymer erosion. A vast number of biodegradable polymers have been synthesized recently and some microorganisms and enzymes capable of degrading them have been identified.
\nThe objective of this chapter is to classification of biodegradable polymers. The chemical structure, sources, production and synthesis methods, physical properties (mechanical, barrier and thermal properties) and applications of most important biodegradable polymers would be discussed.
\nThe biodegradable polymers can be classified according to their chemical composition, origin and synthesis method, processing method, economic importance, application, etc. In the present chapter, biodegradable polymers classified according to their origin into two groups: natural polymers which obtained from natural resources and synthetic polymers which produced from oil. An overview of these categories is given in Fig. 1.
\nBiopolymers are polymers formed in nature during the growth cycles of all organisms; hence, they are also referred to as natural polymers. Their synthesis generally involves enzyme-catalyzed, chain growth polymerization reactions of activated monomers, which are typically formed within cells by complex metabolic processes.
\nSchematic presentation of biobased polymers based on their origin and method of production
For materials applications, the principal polysaccharides of interest are cellulose and starch, but increasing attention is being given to the more complex carbohydrate polymers produced by bacteria and fungi, especially to polysaccharides such as xanthan, curdlan, pullulan and hyaluronic acid. These latter polymers generally contain more than one type of carbohydrate unit, and in many cases these polymers have regularly arranged branched structures. Because of this difference, enzymes that catalyze hydrolysis reactions during the biodegradation of each kind of polysaccharides are different and are not interchangeable.
\nStarch is the major polysaccharide reserve material of photosynthetic tissues and of many types of plant storage organs such as seeds and swollen stems. The principal crops used for its production include potatoes, corn and rice. In all of these plants, starch is produced in the form of granules, which vary in size and somewhat in composition from plant to plant (Chandra and Rustgi, 1998). The starch granule is essentially composed of two main polysaccharides, amylose and amylopectin with some minor components such as lipids and proteins. Amylose is essentially a linear molecule of (1→4)-linked α-D-glucopyranosyl units with some slight branches by (1→6)-α-linkages (Fig. 2). Typically, amylose molecules have molecular weights ranging from 105 to 106 gmol-1 (Buleon et al., 1998). Amylopectin is a highly branched molecule composed of chains of α-D- glucopyranosyl residues linked together mainly by (1→4)-linkages but with (1→6) linkages at the branch points. Amylopectin consist of hundreds of short chains of (1→4)-linked α-D-glucopyranosyl interlinked by (1→6)-α-linkages (Fig. 2). It is an extremely large and highly branched molecule (molecular weights ranging from 106 to 108 gmol-1).
\nThere are three types of crystallinity in starch. They are the \'A\' type mainly cereal starches such as maize, wheat, and rice; \'B\' type such as tuber starches (potato, sago); and finally the \'C\' type crystallinity which is the intermediate between A and B type crystallinity, normally found in bean and other root starches (Blanshard, 1987). Another type of crystallinity is the Vh-type, which is the characteristic of amylose complexed with fatty acids and monoglycerides.
\nStarch granules contain alternating 120-400 nm amorphous and semi-crystalline layers or growth rings (Buleon et al., 1998). The semi-crystalline growth rings are composed of alternating amorphous and crystalline lamellae. The sum of one amorphous and one crystalline lamella is around 9-10 nm in size. Amylopectin is often presumed to support the framework of the semi- crystalline layers in the starch granule. The short chains with polymerization degrees ranging between 15 and 18 form a double helical conformation (Buleon et al., 1998) and associating into clusters. These clusters pack together to produce a structure of alternating crystalline and amorphous lamellar composition. The side chains clusters which are predominantly linear and form double helices are responsible for the crystalline lamellae while the branching regions of the amylopectin molecule are responsible for the amorphous lamellae.
\nChemical structure of amylose and amylopectin (
Thermoplastic starch is plasticized starch that has been processed (typically using heat and pressure) to completely destroy the crystalline structure of starch to form an amorphous thermoplastic starch. Thermoplastic starch processing typically involves an irreversible order- disorder transition termed gelatinization. Starch gelatinization is the disruption of molecular organization within the starch granules and this process is affected by starch-water interactions. Fig. 3 highlights the gelatinization process diagrammatically (Lai and Kokini, 1991). This figure shows raw starch granules made up of amylose (linear) and amylopectin (branched) molecules (step (a)). Then the addition of water breaks up crystallinity and disrupts helices (step (b)). Addition of heat and more water causes granules to swell and amylose diffuses out of the granule (step (c)). Granules, mostly containing amylopectin are collapsed and held in a matrix of amylose (step (d)).
\nThermoplastic starch is produced using dry native starch with a swelling or plastifying agent in compound extruders without adding water. In extrusion, starch is converted by application of both thermal and mechanical energy, and basically three phenomena occur at different structural levels: fragmentation of starch granules; hydrogen bond cleavage between starch molecules, leading to loss of crystallinity; and partial depolymerization of the starch polymers (Fang and Fowler, 2003). Furthermore, the extrusion process ensures the very intimate mixing of the polymers and any additives. By introduction of mechanical energy and heat in a temperature range of 120-220 ˚C, crystal starch, is homogenized and melted in an extrusion process with a plasticizer which lowers the melting point of the starch. With this process, a permanent conversion of the molecular structure to thermoplastic starch is performed (Lorcks, 1998). The thermoplastic starch is free of crystalline fractions. Molecules such as polyglycols (e.g. glycerol, sorbitol, etc), amides and amines serve as non-volatile plasticizers for starch (Wiedersheim and Strobel, 1991).
\nStarch gelatinization process (
Depending on the starch source and processing conditions, a thermoplastic material may be obtained with different properties suitable for various applications. Starch has been widely used as a raw material in film production because of increasing prices and decreasing availability of conventional film-forming resins (Chandra and Rustgi, 1998). Potential applications of starch films include production of disposable food serviceware, food packaging, purchase bags, composting bags and loose fill products (Xiong et al., 2008). Starch is also used in hygiene and cosmetics. Moreover, starch has been used for many years as an additive to plastic for various purposes. Starch was added as filler to various resin systems to make films that were impermeable to water but permeable to water vapour (Pedroso and Rosa, 2005). Starch as biodegradable filler in LDPE was reported (Nakamura et al., 2005). A starch-filled polyethylene film was prepared which becomes porous after the extraction of the starch. This porous film can be readily invaded by microorganisms and rapidly saturated with oxygen, thereby increasing polymer degradation by biological and oxidative pathways. Starch is also useful for making agricultural mulch films because it degrades into harmless products when placed in contact with soil microorganisms. Starch is also used in medical applications. For example, starch-based thermoplastic hydrogels for use as bone cements or drug-delivery carriers have been developed through blending starch with cellulose acetate (Pereira et al., 1998; Espigares et al., 2002).
\nImportant properties of thermoplastic starch based materials include (Lorcks, 1998):
\ncompostable in accordance with DIN 54900
high water vapour permeability
good oxygen barrier
not electrostatically chargeable
low thermal stability
In general, the low resistance to water and the variations in mechanical properties under humid conditions affect the use of starch for various applications. As water has a plasticizing power, the material behavior changes according to the relative humidity of the air (Averous, 2002). Strong hydrophilic character (water sensitivity) and poor mechanical properties compared to conventional synthetic polymers are the most important disadvantages of starch which make it unsatisfactory for some applications such as packaging purposes. Generally, many approaches are suggested to mitigate these shortcomings. One approach is the modification of starch. Cross-linking can be produce low water sensitive and high strength materials (Ghanbarzadeh et al., 2010). According to Takore et al., (2001), the esterification of starch allows the increase of its thermoplastic characteristics, as well as its thermal stability. Other approach to improve the functional properties of the starch films is to blend starch with other polymers. Mao et al. (2000) examined the extrusion of thermoplastic cornstarch- glycerol-polyvinyl alcohol (PVOH) blends and noted the effect of PVOH to improve mechanical properties and slow biodegradation. Development of the polymer nanocomposites is one of the latest revolutionary steps of the polymer technology. In terms of nanocomposite reinforcement of thermoplastic starch polymers there has been much exciting new developments. Dufresne and Cavaille (1998) and Angles and Dufresne (2000) highlight work on the use of microcrystalline whiskers of starch and cellulose as reinforcement in thermoplastic starch polymer and synthetic polymer nanocomposites. They find excellent enhancement of properties, probably due to transcrystallisation processes at the matrix/fibre interface. Almasi et al. (2010) examined the use of nanoscale montmorillonite into starch/carboxymethyl cellulose blends and finds excellent improvements in film impermeability and tensile properties.
\nAt present, cellulose is the most abundant polymer available worldwide with an estimated annual natural production of 1.5 × 1012 tons and considered as an almost inexhaustible source of raw materials (Cao et al., 2009). Cellulose is composed of polymer chains consisting of unbranched β (1→4) linked D- glucopyranosyl units (anhydroglucose unit) (Fig. 4). The length of these β (1→4) glucan chains depends on the source of cellulose. As the main component of cell wall, cellulose is predominantly located in the secondary wall. In the primary cell wall, cellulose consists of roughly 6000 glucose units. Three hydroxyl groups, placed at the positions C2 and C3 (secondary hydroxyl groups) and C6 (primary hydroxyl groups) can form intra- and intermolecular hydrogen bonds (Abdul Khalil et al., 2012). Because of the strong tendency for intra- or intermolecular hydrogen bonding, bundles of cellulose molecules aggregate to microfibrils, which form either highly ordered (crystalline) or less ordered (amorphous) regions (Hamad, 2006). Microfibrils are further aggregated to fibrils and finally to cellulose fibers.
\nCellulose is the main constituent of cell wall in lignocellulosic plant, and its content depends on the plant species, growing environment, position, growth, and maturity. Generally, cellulose content in lignocellulosic plant is 23–53% on a dry-weight basis, less than that in cotton, which is almost made of pure fibrous cellulose. In most straw species, approximately 35–45% of the dry substance is cellulose (Knauf and Moniruzzaman, 2004).
\nChemical structure of cellulose
In the lignocellulosic materials, cellulose is embedded in a gel matrix composed of hemicelluloses, lignins, and other carbohydrate polymers. Cellulose was isolated for the first time some 150 years ago (Smith, 2005). The combination of the chemical and the mechanical treatments is necessary for the dissolution of lignins, hemicelluloses, and other noncellulosic substances. A protocol based on acidified sodium chlorite is frequently applied to delignify woody materials as an initial step in the isolation of cellulose. Alkali extraction to dissolve hemicelluloses before or after delignification is the common method. The presence of high amounts of lignin in isolated cellulose fibers after delignification affects the structure and properties of the cellulose fibers. Fibers with high amounts of lignin are coarse and stiff, and have a brownish color. Therefore, it is challenging to obtain fibers that are relatively free of bound lignin. To achieve this aim, chemical bleaching, which is used to obtain fibers with higher cellulose content from delignified and unbleached fibers, is usually considered as a continuation of delignification process to isolate cellulose from woody raw materials (Brendel et al., 2000). Nowadays, there are various procedures for extraction of cellulose microfibrils (e.g. pulping methods, acid hydrolysis, steam explosion, atc.)(Abdul Khalil et al., 2012).
\nMany useful properties stem from unique functional characteristics related to the chemical structure of cellulose. These structural properties include an extended, planar chain conformation and oriented, parallel-chain packing in the crystalline state. The absence of branches in this 100% linear polymer contributes to efficient chain packing in the native crystalline state, resulting in stiff, dimensionally stable fibers (Smith, 2005). Cellulose fibers thus exhibit a high degree of crystallinity (upwards of 70%) when isolated and purified. However, cellulose fibers present in native woody biomass exhibit approximately 35% crystallinity, due to the presence of other lignocellulosic components (Abdul Khalil et al., 2012). The crystal nature (monoclinic sphenodic) of naturally occurring cellulose is known as cellulose I. Cellulose is resistant to strong alkali (17.5 wt%) but is easily hydrolyzed by acid to water-soluble sugars. Cellulose is relatively resistant to oxidizing agents (John and Thomas, 2008). The tight fiber structure created by hydrogen bonds results in the typical material properties of cellulose, such as high tensile strength and insolubility in most solvents. There are significant differences between the properties of straw cellulose, wood cellulose, and cotton cellulose. The cellulose crystallites are longer in straw pulps than in wood pulps, but they are not as long as in cotton cellulose. In addition, the degree of crystallinity of straw pulps appears to be less than that of wood cellulose. Low crystallinity can be useful when a cellulose derivative is to be manufactured (Sun and Tomkinson, 2000).
\nCellulose has received more attention than any other polymer since it is attacked by a wide variety of microorganisms. The biodegradation of cellulose is complicated, because cellulose exists together with lignin however, it is fortunate that pure cellulose does decompose readily (Chandra and Rustgi, 1998). Fermentation of cellulose has been suggested as a source of chemicals such as ethanol and acetic acid, but this has not achieved any commercial importance to date.
\nThe most significant cellulosic applications are in the paper, wood product, textile, film, and fiber industries but recently it has also attracted significant interest as a source of biofuel production (Mantia and Morreale, 2011). The natural cellulosic carbon skeleton can be utilized in two major applications on an industrial scale. The first is as regenerated or mercerized cellulose (cellulose II, Rayon), which is not moldable and is used only for film and fiber production. The second represents a broader class of applications, which employs chemically modified celluloses, principally the cellulose esters (Chandra and Rustgi, 1998).
\nAs mentioned before, in all forms, cellulose is a very highly crystalline, high molecular weight polymer, which is infusible and insoluble in all but the most aggressive, hydrogen bond-breaking solvents such as
ethers, e.g. methylcellulose, hydroxypropyl methyl cellulose and hydroxylethyl cellulose;
esters, e.g. cellulose acetate, carboxymethyl cellulose and cellulose xanthate, which is used as a soluble intermediate for processing cellulose into either fibre or film forms, during which the cellulose is regenerated by controlled hydrolysis;
acetals, especially the cyclic acetal formed between the C2 and C3 hydroxyl groups and butyraldehyde.
These modified forms of cellulose can be tailored to exhibit particular physical and chemical properties by varying the pattern and degrees of substitution within the cellulose backbone. Industrial applications are numerous and widespread for cellulose derivatives owing to rigidity, moisture vapor permeability, grease resistance, clarity, and appearance (Edgar et al., 2001). Esterification of the cellulose backbone provides structural changes that allow for a greatly expanded range of applications, not available to the parent polysaccharide. Commercially available forms of cellulose acetate have degrees of substitution between 1.7 and 3.0 and are currently used in high volume applications ranging from fibers, to films, to injection moulding thermoplastics, to low solids solvent-borne coatings for metal and automobile industries (Mohanty et al., 2000). Methylcellulose exhibits thermal gelation and has excellent film-forming properties. It has been widely used to prepare edible films (Debeaufort and Voilley, 1997; Peressini et al., 2003). Carboxymethyl cellulose is also widely used in the pharmaceutical and food industries. It has good film forming properties (\n Ghanbarzadeh et al., 2011\n\n ). Carboxymethyl cellulose based film is a very efficient oxygen, carbon dioxide, and lipid barrier. However, it has poor resistance to water vapor transmission (\n Ghanbarzadeh and Almasi, 2011\n ).
\nThe chemically modified celluloses are degradable only under certain circumstances, as more recalcitrant, hydrophobic ester groups replace the native glucopyranosyl hydroxyls (to varying degrees) in the esterification procedure. Structurally, the degrees of substitution and C-2 hydroxyl substitution patterns are important criteria in predicting biodegradation patterns for these polymers (Amass et al., 1998). Biodegradation rates of cellulose esters generally increase with decreasing degrees of acetate substitution.
\nPlant fibers include bast (or stem or soft sclerenchyma) fibers, leaf or hard fibers, seed, fruit, wood, cereal straw, and other grass fibers. All these plant based natural fibers are lignocellulosic in nature and are composed of cellulose, hemicelluloses, lignin, pectin and waxy substances (Kabir et al., 2012). Lignocellulosic biomass comprises approximately 50% of the global biomass and is by far the most abundant renewable organic resource on earth. This woody material is comprised of 30-50% cellulose, 20-50% hemicellulose, and 15-35% lignin, dependent upon the plant species and environmental (growing) conditions (Galbe and Zacchi, 2002). Fig. 5 presents the model of the structural organization of the three major structural constituents of the fiber cell wall (Madsen, 2004). Hemicellulose molecules are hydrogen bonded with cellulose fibrils and they form cementing materials for the fiber structure. Lignin and pectin are coupled with the cellulose–hemicellulose network and provides an adhesive quality to hold the molecules together. This adhesive quality is the cause for the strength and stiffness properties of the fiber.
\nHemicellulose is not a form of cellulose and the name is a misnomer. They comprise a group of polysaccharides composed of a combination of 5- and 6-carbon ring sugars (Fig. 6a). Hemicellulose differs from cellulose in three aspects. Firstly, they contain several different sugar units whereas cellulose contains only 1,4–β-D-glucopyranose units. Secondly, they exhibit a considerable degree of chain branching containing pendant side groups giving rise to its non crystalline nature, whereas cellulose is a linear polymer. Thirdly, the degree of polymerization of native cellulose is 10–100 times higher than that of hemicelluloses (John and Thomas, 2008). The degree of polymerization (DP) of hemicellulose is around 50–300. Hemicellulose is very hydrophilic, soluble in alkali and easily hydrolyzed in acids.
\nLignin is a complex hydrocarbon polymer with both aliphatic and aromatic constituents (Fig. 6b). They are totally insoluble in most solvents and cannot be broken down to monomeric units. Lignin is totally amorphous and hydrophobic in nature. It is the compound that gives rigidity to the plants. It is thought to be a complex, three-dimensional copolymer of aliphatic and aromatic constituents with very high molecular weight. Hydroxyl, methoxyl and carbonyl groups have been identified. Lignin has been found to contain five hydroxyl and five methoxyl groups per building unit. It is believed that the structural units of lignin molecule are derivatives of 4-hydroxy-3-methoxy phenylpropane (John and Thomas, 2008). The main difficulty in lignin chemistry is that no method has been established by which it is possible to isolate lignin in its native state from the fiber. Lignin is considered to be a thermoplastic polymer exhibiting a glass transition temperature of around 90 ˚C and melting temperature of around 170 ˚C (Olesen and Plackett, 1999). It is not hydrolyzed by acids, but soluble in hot alkali, readily oxidized, and easily condensable with phenol.
\nPectins are a collective name for heteropolysaccarides. They give plants flexibility. Pectin, is a complex anionic polysaccharide composed of β-1,4-linked D-galacturonic acid residues, where in the uronic acid carboxyls are either fully (HMP, high methoxy pectin) or partially (LMP, low methoxy pectin) methyl esterified (Tharanathan, 2003) (Fig. 6c).
\nStructural organization of the three major constituents in the fiber cell wall (
It has been proposed various techniques for separation of these components from lignocellulosic complex. The \'Clean Fractionation Process\', developed and patented by the National Renewable Energy Laboratory (Golden, CO, USA), is one example of an organic solvent-based system used to separate and purify the three major feedstocks present in lignocellulosic biomass (Smith, 2005). Lignin and hemicellulose are disrupted and solubilized in the solvent mixture composed of water, methyl-isobutyl-ketone (MIBK), ethanol, and sulfuric acid (H2SO4), following a steam explosion treatment catalyzed by the acidic conditions created within the reactor due to the added sulfuric acid and endogenous acetic acid released during the hydrolysis. This environmentally benign process selectively separates cellulose, hemicellulose, and lignin with a high degree of purity, substantial energy savings, and lessened production cost (Kulesa, 1999).
\nChemical structure of hemicelluloses, lignin and pectins (
These lignocellulosic materials have the potential to be utilized as a feedstock for the production of a wide variety of industrial and commodity products, ranging from paper, lumber, and platform chemicals to a variety of fuels and advanced materials, including biodegradable polymers (Smith, 2005). For example, HMP forms excellent films. Plasticized blends of citrus pectin and high amylase starch give strong, flexible films, which are thermally stable up to 180˚C. Pectin is also miscible with poly(vinyl alcohol) in all proportions. Potential commercial uses for such films are water soluble pouches for detergents and insecticides, flushable liners and bags, and medical delivery systems and devices (Tharanathan, 2003).
\nHemicellulose can be utilized in microbial fermentations for the production of a variety of value-added products. Detoxified hemicellulosic hydrolysates have been used as xylose-rich feedstocks in a variety of biotechnological applications including the microbial production of ethanol, xylitol, and biodegradable polyhydroxyalkanoate (PHA) polymers (Smith, 2005). Production of PHAs based on renewable, bio-based substrates could make PHA-derived thermoplastic products more economically competitive with petroleum-based plastics, as the major costs in PHA production are the carbon source and the separation process. In the next section of this chapter we will describe the properties of this family of degradable microbial polyesters.
\nChitin is a polysaccharide found in the shells of crabs, lobsters, shrimps and insects or can be generated via fungal fermentation processes. Chitosan is the deacylated derivative of chitin and forms the exoskeleton of arthropod. Structurally chitosan is a linear polysaccharide consisting of
Chitin is insoluble in its native form but chitosan, is water soluble. Chitosan is soluble in weekly acidic solutions resulting in the formation of a cationic polymer with a high charge density and can therefore form polyelectrolyte complexes with wide range of anionic polymers (Pachence et al., 2007). Chitosan has been found to be non-toxic after oral administration in humans and is an FDA approved food additive (Nair and Laurencin, 2007).
\nEnzymes, such as chitosanase, lysozyme and papain are known to degrade chitosan
Chemical structure of chitin and chitosan (Nair and Laurencin, 2007).
These biopolymers are biocompatible and have antimicrobial activities as well as the ability to absorb heavy metal ions. They also find applications in the cosmetic industry because of their water-retaining and moisturizing properties (Chandra and Rustgi, 1998). Chitosan has been formed into membranes and matrices suitable for several tissue-engineering applications (Shalaby et al., 2004). Chitin derivatives can also be used as drug carriers. Chitosan was used to develop injectable thermo-sensitive carrier material for biomedical applications. Due to the mild gelling conditions, the hydrogel has been found to be a potential delivery vehicle for growth factors, small molecular weight drugs and cells for localized therapy (Nair et al., 2006). The high chemical reactivity of chitosan, has also led to several chitosan-drug conjugates for cancer therapy (Onishi et al., 2001). Chitosan gels, powders, films, and fibers have been formed and tested for many applications such as encapsulation, membrane barriers, contact lens materials, cell culture, and inhibitors of blood coagulations (Pachence et al., 2007). Chitosan has good film forming properties and therefore can be used as a food packaging material (Ghanbarzadeh et al., 2008; Suyatama et al., 2005).
\nGums are a group of polysaccharides that can form gels in solution upon the introduction of counterions. The degree of cross-linking is dependent on various factors such as pH, type of counterion, and the functional charge density of these polymers (Chandra and Rustgi, 1998). The common types of these polysaccharides will be discussed here.
\nAlginic acid present within the cell walls and intercellular spaces of brown algae and has a structural role in giving flexibility and strength to marine plants. Alginate is a non-branched, binary copolymer of (1-4) glycosidically linked
Proteins can be defined as natural polymers able to form amorphous three-dimensional structures stabilized mainly by noncovalent interactions. The functional properties of these materials are highly dependent on structural heterogeneity, thermal sensitivity, and hydrophilic behavior of proteins. Numerous vegetable and animal proteins are commonly used as biodegradable polymers.
\nZein comprises a group of alcohol-soluble proteins (prolamins) found in corn endosperm. It accounts for 50% or more of total endosperm protein, and its only known role is the storage of nitrogen for the germinating embryo (Gennadios, 2002). It can be extracted with aqueous alcohol and dried to a granular powder. Based on solubility differences, zein consists of three protein fractions, i.e., α-zein, β-zein, and γ-zein. α-Zein accounts for 75 to 85% of the total protein and is dominated by two groups of proteins, Z19 and Z22, according to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS PAGE) (Argos et al., 1982). Z19 and Z22 consist of 210 and 245 amino acids, respectively.
\nDue to the hydrophobic nature of zein, water sorption is extremely low in the low water activity (
The film-forming properties of zein have been recognized for decades, and they are the basis for its commercial utilization (Lai et al., 1997). Coating films are formed on hard surfaces by covering them with zein solutions and allowing the solvent to evaporate off. The dried zein residue forms hard and glossy, scuff-proof, protective coatings that also are resistant to microbial attack (Reiners et al., 1973). Zein coatings are used as oxygen, lipid, and moisture barriers for nuts, candies, confectionery products, and other foods. Rice fortified with vitamins and minerals has been coated with zein/stearic acid/wood resin mixtures to prevent vitamin and mineral losses during washing in cold water. Pharmaceutical tablets are zein-coated for controlled ingredient release and protection (Gennadios, 2002). Use of zein-based coatings has been suggested for reducing oil uptake by deep-fat fried foods, for protecting active ingredients in chewing gum, for achieving controlled release of active ingredients in pharmaceutical tablets and for masking the taste of orally administered drugs (Gennadios, 2002). Zein, upon casting from aqueous aliphatic alcoholic solutions forms tough, glossy and grease resistant films (Ghanbarzadeh et al., 2006c; 2007b). By cross-linking the tensile strength of the films is further improved. Zein films have water vapor permeability (WVP) values lower than or similar to those of other protein films, cellulose ethers, and cellophane (Krochta, 1992). However, their WVP is notably higher than that of LDPE or ethylene-vinyl alcohol copolymer (EVOH) (Gennadios, 2002).
\nWhereas dry wheat flour comprises 9–13% protein and 75–80% starch, wheat gluten consists mainly of wheat storage protein (70–80%, dry matter basis) with traces of starch and non -starch polysaccharides (10–14%), lipids (6–8%), and minerals (0.8–1.4%). Osborne distinguished four wheat protein classes based on their solubility in different solvents, namely, albumins, globulins, gliadins, and glutenins. The albumins and globulins (15–22% of total protein), which are, respectively, water- and salt-soluble, are removed with starch granules during gluten processing. In contrast, the gliadins, which are alcohol-soluble, and the glutenins, which are soluble (or at least dispersible) in dilute acid or alkali solutions, are being collected into gluten (Wrigley and Bietz, 1988). Gliadin molecules may interact together or with glutenin molecules via hydrophobic interactions and hydrogen bonds. In the fully hydrated state, gliadin exhibits viscous flow properties without significant elasticity. For cereal technologists, gliadin accounts for the extensibility of wheat flour dough and acts as a filler diluting glutenin interactions. Contrary to gliadin, which is comprised of distinct polypeptide chains, glutenin consists of polymers made from polypeptide chains (also named subunits) linked end-to-tail by SS bonds (Kasarda, 1999). Vital wheat gluten is the cohesive and elastic mass that is leftover after starch is washed away from wheat flour dough. Commercially, it is an industrial by-product of wheat starch production via wet milling (Gennadios, 2002).
\nWheat gluten is suitable for numerous food and nonfood uses. Its main application is in the bakery industry, where it is used to strengthen weak flours rendering them suitable for bread baking (Gennadios, 2002). The other potential applications of gluten are very diverse: windows in envelopes, surface coatings on paper, biodegradable plastic films for agricultural uses, water-soluble bags with fertilizers, detergents, cosmetics, cigarette filters and additives and molded objects (Cuq et al., 1998). Wheat gluten-based materials are homogeneous, transparent, mechanically strong, and relatively water resistant. They are biodegradable and a priori biocompatible, apart from some wheat gluten-specific characteristics such as allergenicity (Guilbert et al., 1996).
\nThe moisture barrier properties of wheat gluten-based films are relatively poor as compared to synthetic films, such as LDPE. The gas (O2, CO2, and ethylene) barrier properties of wheat gluten-based films are highly interesting, as they are exceptionally good at low relative humidity (RH) conditions. Water and other plasticizers can lower the glass transition temperature (Tg) of the wheat gluten and enable processing at temperatures below those that lead to protein decomposition, which means that protein-based films can be formed by using techniques that are conventionally used with synthetic polymers (e.g., extrusion, injection, and molding) (Gennadios, 2002).
\nThe major use of soybean in the food industry is as a source of oil, while soy protein concentrate and isolate are readily available as co-products of the oil processing industry (Pszczola, 1998). Soy protein is a complex mixture of proteins with widely different molecular properties. The major soybean proteins have molecular weights ranging from 200 to 600 kDa. Most soy proteins (~90%) are globulins, which can be fractionated into 2S, 7S, 11S and 15S according to their sedimentation coefficients. The 7S and11S fractions, the main fractions making up about 37% and 31% of the total extractable protein, have the capability of polymerization (Wolf, 1972). Soy protein used in the food industry is classified as soy flour, concentrate, or isolate based on the protein content. Soy flour contains 50–59% protein and is obtained by grinding defatted soy flakes. Soy protein concentrate contains 65–72% protein and is obtained by aqueous liquid extraction or acid leaching process. Soy protein isolate contains more than 90% protein and is obtained by aqueous or mild alkali extraction followed by isoelectric precipitation (Soy Protein Council, 1987).
\nSoy protein is an abundant and relatively cheap ingredient source for various food applications. The functional properties that make soy protein useful in foods include cohesiveness, adhesiveness, emulsification, dough and fiber formation, whippability, solubility, and foaming (Gennadios, 2002). Soy protein also is used in infant formulas and in baked, meat, and dairy products (Witherly, 1990). The use of soy protein as a film-forming agent can add value to soybeans by creating new channels for marketing soy proteins (Cao et al., 2007). Soy protein is a viable and renewable resource for producing edible and environmentally friendly biodegradable films. Soy protein films are flexible, smooth, transparent, and clear compared to other films from plant proteins (Cho and Rhee, 2004). These films have good mechanical properties but they are generally slightly water-resistant (Cuq et al., 1998). Soy protein films are typically prepared by drying thin layers of cast film-forming solutions (Gennadios, 2002). Biodegradable plastics were also produced from soy isolate and concentrate by a thermomolding process (Jane et al., 1994).
\nCollagen is an abundant protein constituent of connective tissue in vertebrate (about 50% of total human protein) and invertebrate animals. Similar to cellulose in plants, collagen molecules support mechanical stresses transferred to them by a low-modulus matrix (Gennadios, 2002). Collagen is a rod-type polymer nearly 300 nm long with a molecular weight of 300,000. There have been more than twenty two different types of collagen identified so far in the human body, with the most common being Type I–IV. Type I collagen is the single most abundant protein present in mammals and is the most thoroughly studied protein. The Type I collagen is composed of three polypeptide subunits with similar amino acid compositions. Each polypeptide is composed of about 1050 amino acids, containing approximately 33% glycine, 25% proline and 25% hydroxyproline with a relative abundance of lysine (Nair and Laurencin, 2007). Collagen is a hydrophilic protein because of the greater content of acidic, basic, and hydroxylated amino acid residues than lipophilic residues. Therefore, it swells in polar liquids with high solubility parameters.
\nCollagen undergoes enzymatic degradation within the body via enzymes, such as collagenases and metalloproteinases, to yield the corresponding amino acids. Due to their enzymatic degradability, unique physico-chemical, mechanical and biological properties collagen has been extensively investigated for various applications. Collagen is mostly soluble in acidic aqueous solutions and can be processed into different forms such as sheets, tubes, sponges, foams, nanofibrous matrices, powders, fleeces, injectable viscous solutions and dispersions. Studies have also shown that the degradation rate of collagen used for biomedical applications can be significantly altered by enzymatic pre-treatment or cross-linking using various cross-linking agents (Nair and Laurencin, 2007).
\nType I collagen is found in high concentrations in tendon, skin, bone, and fascia, which are consequently convenient and abundant sources for isolation of this natural polymer. The major sources of collagen currently used for industrial applications are bovine or porcine skin or bovine or equine Achilles tendons (Pachence et al., 2007). Thermal or chemical dissociation of collagen polypeptide chains forms products known as gelatin. Insoluble collagen is converted to soluble gelatin by acid or alkaline/lime (mild and slow) processing. Two processes are mainly used for commercial production of gelatin. In the first process, the collagen in hide or demineralized bone is partly depolymerized by prolonged liming that breaks down covalent cross-links. The occurring hydrolysis results in extensive release of collagenous material, which is solubilized at near neutral pH at temperatures of 60–90 ˚C (Type B gelatin). The acid process (Type A gelatin) involves soaking skin or bone in a dilute acid followed by extraction at acid pH (Johnston-Banks, 1990).
\nThe properties of collagen and gelatin are of great interest to various fields, such as surgery (implantations; wound dressings), leather chemistry (tanning), pharmacy (capsule production; tablet binding), and food science (gels; edible films) (Arvanitoyannis et al., 1998). Reportedly, about 65% of gelatin manufactured worldwide is used in foods, 20% in photographic applications, 10% in pharmaceutical products, and 5% in other specialized and industrial applications (Slade and Levine, 1987). Collagen has been extensively investigated for the localized delivery of low molecular weight drugs including antibiotics (Gruessner et al., 2001). Collagen films have traditionally been used for preparing edible sausage casing (Hood, 1987). Gelatin has been successfully used to form films that are transparent, flexible, water-resistant, and impermeable to oxygen (Hebert and Holloway 1992). These films were made by cooling and drying an aqueous film-forming solution based on gelatin. Gelatin is also used as a raw material for photographic films, and to microencapsulate aromas, vitamins, and sweeteners (Balassa and Fanger 1971).
\nCasein is the main protein of milk, representing 80% of the total milk proteins, it is a phosphoprotein that may be separated into various electrophoretic fractions, αs1-casein, αs2-casein, β-casein and κ-casein which differ in primary, secondary and tertiary structure and molecular weight. These four different types of casein are found in bovine milk in the approximate ratio of 4:1:4:1 respectively (Dalgleish, 1997). Casein exists in the form of micelles containing all four casein species complexed with colloidal calcium phosphate. The casein micelles are stable to most common milk processes such as heating, compacting, and homogenization. Micellar integrity is preserved by extensive electrostatic and hydrogen bonding, and hydrophobic interactions (Gennadios, 2002).
\nTwo principal methods have been established for the production of casein on commercial scale, i.e., isoelectric precipitation (acid casein) and enzymatic coagulation (rennet casein). The preparation of acid casein from skim milk is quite simple. The essential steps involve acidification to about pH 4.6 (isoelectric point of casein) to induce the coagulation of casein, adjustment of temperature to between 30 and 40 ˚C for better handling properties of the product, washing, pressing or centrifuging the curd to remove excessive water, and finally drying and grinding. Hydrochloric acid is usually used for both laboratory scale and industrial preparation of casein. The key difference in producing rennet casein from producing acid casein is the means of coagulation. A proteolytic enzyme, such as chymosin (rennin), cleaves the -casein fraction to release a glycomacropeptide, thus destabilizing the casein micelles and promoting coagulation of casein in the presence of calcium cations (Gennadios, 2002). Though water-insoluble casein has some applications, most food application would require casein with high water solubility. This is achieved by dispersing the casein in water and adjusting the pH to between 6.5 and 7.0 with an alkali. The most commonly used soluble caseinate is sodium caseinate. It is normally manufactured by dissolving fresh acid casein curd in sodium hydroxide followed by spray drying. Other soluble caseinates prepared in a similar manner include potassium, calcium, magnesium, and ammonium caseinates (Fox and McSweeney, 1998).
\nIts relative simple isolation and the useful properties of casein as an industrial material and food ingredient have led to commercial production of casein and caseinates since the 19th century (Muller, 1982). Casein and caseinates are suitable for numerous food and nonfood uses such as in industrial applications (especially in glues, paper coatings, paints, leather finishing, textile fibers, and plastics), and in various food products. The end-uses of casein and caseinates have gradually shifted from industrial to food applications. About 70 to 80% of the casein produced worldwide is used as a food ingredient (Gennadios, 2002). Film-forming properties of caseins have been used to improve the appearance of numerous foods, to produce water-soluble bags, and to produce origin or quality identification labels inserted under precut cheeses, to ensure the surface retention of additives on intermediate-moisture foods, and to encapsulate polyunsaturated lipids for animal feeds (Cuq et al., 1998). Casein-based edible films are attractive for food applications due to their high nutritional quality, excellent sensory properties and potential to adequately protect food products from their surrounding environment (Fabra et al., 2009). The mechanical properties of casein and caseinate films, being neither too tough nor too fragile, also make them suitable for edible purposes. Though more permeable to water vapor than plastic films, they are capable of retarding moisture transfer to some degree (Buonocore et al., 2003). Casein and caseinate films dissolve nearly instantaneously in water and this is desirable for many food applications.
\nWhey proteins are those proteins that remain in milk serum after pH/rennet coagulation of casein during cheese or casein manufacture (Gennadios, 2002). Whey protein, which represents approximately 20% of total milk proteins is a mixture of proteins with diverse functional properties. The five main proteins are α-lactalbumins, β-lactoglobulins, bovine serum albumin, immunoglobulins, and proteose peptones. α-lactalbumins, β-lactoglobulins and bovine serum albumin comprise 57, 19 and 7% of the total whey protein. The immunoglobulins and proteose-peptone fractions represent the remainder of the whey protein. Whey proteins are globular and soluble at pH 4.6 (Dybing and Smith, 1991).
\nThe industrial processes used for whey protein recovery are ultrafiltration, reverse osmosis, gel filtration, electrodialysis, and ion exchange chromatography. Fractionated whey constituents of various degrees of concentration can be obtained by combining two or more of the above recovery processes (Sienkiewicz and Riedel, 1990). Whey protein products can be classified according to their composition. In particular, they are divided according to their protein content. Whey protein concentrate (WPC) contains 25–80% protein. Whey protein isolate (WPI) is nearly all protein (>90%) (Gennadios, 2002).
\nWhey protein, a byproduct of the cheese industry, has excellent nutritional and functional properties and the potential to be used for human food and animal feed. The film-forming properties of whey proteins have been used to produce transparent, flexible, colorless, and odorless films, such as those produced from caseins (Cuq et al., 1998). The use of whey proteins to make an edible packaging film material brings several environmental advantages because of the film’s biodegradability and its capacity to control moisture, carbon dioxide, oxygen, lipid, flavor and aroma transfer (Ozdemir and Floros, 2008). These properties offer the potential to extend the shelf-life of many food products, avoiding quality deterioration (Gounga et al., 2007).
\nPrevalent types of plant and animal proteinous biopolymers have been discussed previously. Nevertheless, these are not the only available biopolymers from this category. There are other proteins, which have potential to use as biopolymeric materials for different applications. Most important of them includes elastin (a major protein component of vascular and lung tissue), egg albumins, fish myofibrillar protein and wool keratin (Gennadios, 2002).
\nAn interesting property of elastin is its ability to undergo folding when the temperature is increased above 25 ˚C. This is due to its transition from a disordered form to an ordered form at higher temperature called inverse temperature transition (ITT). Due to the unique thermal transition properties of elastin, it has been extensively investigated as a smart, injectable drug delivery system (Mithieux et al., 2004). Use of egg albumins to encapsulate organic hydrophobic compounds in cosmetics and foods has been proposed in many patents (Gennadios et al., 1999). The potential use of myofibrillar proteins for the preparation of films and coatings was proposed to identify new practical applications for fish proteins (Shiku et al., 2004). Yamauchi et al (1996) developed water-insoluble films based on keratin by casting and drying alkaline dispersions. The large amount of cystine in keratin favors formation of many disulfide bonds that could stabilize the proteic network. However, because of their unpleasant mouthfeel, edible coatings based on keratin have not found many applications (Yamauchi and Khoda, 1997).
\nOther proteins have been also used for various purposes, including proteins from rye, pea, barley, sorghum, rice, sunflower, pistachio and peanut (Gennadios, 2002).
\nThe microbial polyesters are produced by biosynthetic function of a microorganism and readily biodegraded by microorganisms and within the body of higher animals, including humans. In the field of medicine, they can be used as implanting material and a drug carriers.
\nPolyhydroxyalkanoates (PHAs) are a family of intracellular biopolymers synthesized by many bacteria as intracellular carbon and energy storage granules (Fig. 8). Depending on growth conditions, bacterial strain, and carbon source, the molecular weights of these polyesters can range from tens into the hundreds of thousands (Pachence et al., 2007). Bacterially synthesized PHAs have attracted attention because they can be produced from a variety of renewable resources and are truly biodegradable and highly biocompatible thermoplastic materials.
\nThe plastic-like properties and biodegradability of PHAs offer an attraction as a potential replacement for non-degradable polyethylene and polypropylene. Many efforts have been made to produce PHA as environmentally degradable thermoplastics (Chen et al., 2001; Chen and Page, 1997; Byrom, 1992). PHAs have a promising potential for food packaging applications. However, due to exorbitant production costs, few suppliers exist in the market.
\nOver 90 different types of PHA consisting of various monomers have been reported and the number is increasing. Some PHAs behave similarly to conventional plastics such as polyethylene and polypropylene, while others are elastomeric (Yang et al., 2002). The most representative member of this family is poly(3-hydroxybutyrate) (PHB).
\nChemical structure of polyhydroxyalkanoate (
Among the PHA family, poly-3-hydroxybutyrate (PHB) is the most common member; it belongs to the short chain length PHA with its monomers containing 4-5 carbon atoms (Smith, 2005). ICI developed a biosynthetic process for the manufacture of PHB, based on the fermentation of sugars by the bacterium
PHB has the poorest mechanical properties compared with its copolymers. Efforts have been made to improve the mechanical properties of PHB; Iwata et al. (2003) prepared uniaxially oriented films of PHB, with sufficient strength and flexibility by cold-drawing from an amorphous preform at a temperature below, but near to, the glass transition temperature. Melt crystallized and solvent-cast films of PHB are usually quite brittle, and the orientation is critical and difficult to reproduce consistently.
\nSince current production technology is still unable to produce any PHB that is competitive with conventional plastics such as polyethylene, polypropylene or polystyrene which are manufactured on a large scale, the application of PHB as environmentally friendly packaging materials is still unrealistic. Therefore, increasing research is focused on the biosynthesis of PHB with unconventional structures that may bring new properties and new applications for PHB (Eggink et al., 1995; Lutke-Eversloh, et al., 2001; Kim, et al., 1996).
\nPHB has been found to have low toxicity, in part due to the fact that it degrades
Blends of the PHB family are usually compatible and co-crystallization is enhanced. Yoshie et al., (2004) studied solid-state structures and crystallization kinetics of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB/HV) blends. It was found that PHB and HV can co-crystallize.
\nThe copolymers of PHB with hydroxyvaleric acid (PHB/HV) (Fig. 9) are less crystalline, more flexible, and more readily processible, but they suffer from the same disadvantage of being too hydrolytically stable to be useful in short-term applications when resorption of the degradable polymer within less than one year is desirable.
\nPHB and its copolymers with up to 30% of 3-hydroxyvaleric acid are now commercially available under the trade name Biopol. It was found previously that a PHA copolymer of PHB/HV with a 3- hydroxyvalerate content of about 11%, may have an optimum balance of strength and toughness for a wide range of possible applications (Pachence et al., 2007).
\nPoly(
Poly (ε-caprolactone) (PCL) (Fig. 10) is aliphatic polyester and is of great interest as it can be obtained by the ring opening polymerization of a relatively cheap monomeric unit ‘ε-caprolactone’. This polyester is highly processible as it is soluble in a wide range of organic solvents (Nair and Laurencin, 2007).
\nPCL exhibits several unusual properties not found among the other aliphatic polyesters. Most noteworthy are its exceptionally low glass transition temperature of -62°C and its low melting temperature of 57°C. Another unusual property of PCL is its high thermal stability. Whereas other tested aliphatic polyesters had decomposition temperatures (
PCL undergoes hydrolytic degradation due to the presence of hydrolytically labile aliphatic ester linkages; however, the rate of degradation is rather slow (2–3 years). PCL is a semicrystalline polymer with a low glass transition temperature of about -60°C. Thus, PCL is always in a rubbery state at room temperature. Among the more common aliphatic polyesters, this is an unusual property, which undoubtedly contributes to the very high permeability of PCL for many therapeutic drugs (Pitt et al., 1987). Due to the slow degradation, high permeability to many drugs and non-toxicity, PCL was initially investigated as a long-term drug/vaccine delivery vehicle. Extensive research is ongoing to develop various micro- and nano-sized drug delivery vehicles based on PCL (Sinha et al., 2004). Due to its excellent biocompatibility, PCL has also been extensively investigated as scaffolds for tissue engineering.
\nChemical structure of polyhydroxyalkanoate (
Bacterial cellulose (BC) belongs to specific products of primary metabolism and is mainly a protective coating, whereas plant cellulose (PC) plays a structural role. Cellulose is synthesized by bacteria belonging to the genera
Extensive research on BC revealed that it is chemically identical to PC, but its macromolecular structure and properties differ from the latter (Fig. 11). Nascent chains of BC aggregate to form subfibrils, which have a width of approximately 1.5 nm and belong to the thinnest naturally occurring fibers, comparable only to subelemental fibers of cellulose detected in the cambium of some plants and in quinee mucous. BC subfibrils are crystallized into microfibrils, these into bundles, and the latter into ribbons (Bielecki, 2004). Dimensions of the ribbons are 3-4 (thickness) \n
BC is also distinguished from its plant counterpart by a high crystallinity index (above 60%) and different degree of polymerization (DP), usually between 2000 and 6000, but in some cases reaching even 16,000 or 20,000, whereas the average DP of plant polymer varies from 13,000 to 14,000 (Iguchi et al., 2000).
\nOne of the most important features of BC is its chemical purity, which distinguishes this cellulose from that from plants, usually associated with hemicelluloses and lignin, removal of which is inherently difficult (Bielecki, 2004). Because of the unique properties, resulting from the ultrafine reticulated structure, BC has found a multitude of applications in paper, textile, and food industries, and as a biomaterial in cosmetics and medicine (Hu et al., 2011). Wider application of this polysaccharide is obviously dependent on the scale of production and its cost. Therefore, basic studies run together with intensive research on strain improvement and production process development.
\nSchematic model of BC microfibrils (Right) drawn in comparison with the `fringed micelles\'; of PC fibrils (Left)(
This category of biopolymers belongs to biodegradable polyesters and produced by polycondensation or ring-opening polymerization of biologically derived monomers.
\nAmong the family of biodegradable polyesters, polylactides (i.e. PLA) have been the focus of much attention because they are produced from renewable resources such as starch, they are biodegradable and compostable, and they have very low or no toxicity and high mechanical performance, comparable to those of commercial polymers (Yu et al., 2006).
\nPLA or poly-lactide was discovered in 1932 by Carothers. He was only able to produce a low molecular weight PLA by heating lactic acid under vacuum while removing the condensed water. The problem at that time was to increase the molecular weight of the products; and, finally, by ring-opening polymerization of the lactide, high-molecular weight PLA was synthesized (Jamshidian et al., 2010). PLA was 1st used in combination with polyglycolic acid (PGA) as suture material and sold under the name Vicryl in the U.S.A. in 1974 (Mehta et al., 2005).
\nLactic acid (2-hydroxy propionic acid), the single monomer of PLA (Fig. 12), is produced via fermentation or chemical synthesis. Its 2 optically active configurations, the L(+) and D(−) stereoisomers are produced by bacterial (homofermentative and heterofermentative) fermentation of carbohydrates. The homofermentative method is preferably used for industrial production because its pathways lead to greater yields of lactic acid and to lower levels of by-products. The general process consists of using species of the
The polymerization of racemic (D,L)-lactide and mesolactide, results in the formation of amorphous polymers. Among these monomers, L-lactide is the naturally occurring isomer. Similar to polyglycolide, poly(L-lactide) (PLLA) is also a crystalline polymer (~37% crystallinity) and the degree of crystallinity depends on the molecular weight and polymer processing parameters. It has a glass transition temperature of 60–65 ºC and a melting temperature of approximately 175 ºC (Nair et al., 2007).
\nPoly (L-lactide) is a slow-degrading polymer compared to polyglycolide, has good tensile strength, low extension and a high modulus (approximately 4.8 GPa) and hence, has been considered an ideal biomaterial for load bearing applications, such as orthopaedic fixation devices. It is classified as generally recognized as safe (GRAS) by the United State Food and Drug Administration (FDA) and is safe for all food packaging applications (Madhavan Nampoothiri et al., 2010; FDA 2002).
\nPolylactides undergo hydrolytic degradation via the bulk erosion mechanism by the random scission of the ester backbone. It degrades into lactic acid a normal human metabolic by-product, which is broken down into water and carbon dioxide via the citric acid cycle (Maurus and Kaeding, 2004).
\nChemical structure of polylactic acid (
Polyglycolide or Polyglycolic acid (PGA) is a biodegradable, thermoplastic polymer and the simplest linear, aliphatic polyester (Fig. 13).
\nPGA has been known since 1954 as a tough fiber-forming polymer (Pachence et al., 2007).
\nPolyglycolide has a glass transition temperature between 35-40 °C and its melting point is reported to be in the range of 225-230 °C. PGA also exhibits an elevated degree of crystallinity, around 45-55%, thus resulting in insolubility in water. The solubility of this polyester is somewhat unique, in that its high molecular weight form is insoluble in almost all common organic solvents (acetone, dichloromethane, chloroform, ethyl acetate, tetrahydrofuran), while low molecular weight oligomers sufficiently differ in their physical properties to be more soluble. However, polyglycolide is soluble in highly fluorinated solvents like hexafluoroisopropanol (HFIP) and hexafluoroacetone sesquihydrate, that can be used to prepare solutions of the high molecular weight polymer for melt spinning and film preparation. Fibers of PGA exhibit high strength and modulus (7 GPa) and are particularly stiff (Nair et al., 2007).
\nPolyglycolide is characterized by hydrolytic instability owing to the presence of the ester linkage in its backbone. The degradation process is erosive and appears to take place in two steps during which the polymer is converted back to its monomer glycolic acid: first water diffuses into the amorphous (non-crystalline) regions of the polymer matrix, cleaving the ester bonds; the second step starts after the amorphous regions have been eroded, leaving the crystalline portion of the polymer susceptible to hydrolytic attack. Upon collapse of the crystalline regions the polymer chain dissolves (Tian et al., 2012).
\nThe traditional role of PGA as a biodegradable suture material has led to its evaluation in other biomedical fields. Implantable medical devices have been produced with PGA, including anastomosis rings, pins, rods, plates and screws. It has also been explored for tissue engineering or controlled drug delivery. Tissue engineering scaffolds made with polyglycolide have been produced following different approaches, but generally most of these are obtained through textile technologies in the form of non-woven meshes. The Kureha Corporation has announced its commercialization of high molecular weight polyglycolide for food packaging applications under the trade name of Kuredux®. Production is at Belle, West Virginia, with an intended capacity of 4000 annual metric tons, according to a Chemicals Technology report. Its attributes as a barrier material result from its high degree of crystallization. A low molecular weight version (approximately 600 amu) is available from the DuPont Co. and is purported to be useful in oil and gas applications (Chandra and Rustgi, 1998).
\nOwing to its hydrolytic instability, however, its use has initially been limited. Currently polyglycolide and its copolymers (poly(lactic-
Chemical structure of polyglycolic acid (
Polyester is a category of polymers which contain the ester functional group in their main chain. Aliphatic polyesters are biodegradable but often lack in good thermal and mechanical properties. Vice versa, aromatic polyesters, like Polyethylene terephthalate (PET), have excellent material properties, but are resistant to microbial attack. Among biodegradable polymers, aliphatic polyester-based polymeric structures are receiving special attention because they are all more or less sensitive to hydrolytic degradation, a feature of interest when compared with the fact that living systems function in aqueous media. Only some of these aliphatic polyesters are enzymatically degradable. A smaller number is biodegradable, and an even more limited number is biorecyclable.
\nPoly (glycolic acid) (PGA), poly (lactic acid) (PLA), and their copolymers are the most widely used synthetic degradable polymers in medicine. In section 2.1.2.3., structure and properties of these polymers has been explained and in this section synthetic production of them are presented. PGA can be obtained through several different processes starting with different materials (Nair et al., 2007):
\npolycondensation of glycolic acid;
ring-opening polymerization of glycolide;
solid-state polycondensation of halogenoacetates
Polycondensation of glycolic acid is the simplest process available to prepare PGA, but it is not the most efficient because it yields a low molecular weight product. The most common synthesis used to produce a high molecular weight form of the polymer is ring-opening polymerization of "glycolide", the cyclic diester of glycolic acid.
\nIn spite of its low solubility, this polymer has been fabricated into a variety of forms and structures. Extrusion, injection and compression molding as well as particulate leaching and solvent casting, are some of the techniques used to develop polyglycolide-based structures for biomedical applications (Gunatillake et al., 2006).
\nThe high rate of degradation, acidic degradation products and low solubility however, limit the biomedical applications for PGA. Therefore, several copolymers containing glycolide units are being developed to overcome the inherent disadvantages of PGA.
\nDue to its hydrophilic nature, surgical sutures made of PGA tend to lose their mechanical strength rapidly, typically over a period of two to four weeks post-implantation. In order to adapt the materials properties of PGA to a wider range of possible applications, researchers undertook an intensive investigation of copolymers of PGA with the more hydrophobic PLA. Alternative sutures composed of copolymers of glycolic acid and lactic acid is currently marketed under the trade names Vicryl and Polyglactin 910 (Pachence et al., 2007).
\nDue to the presence of an extra methyl group in lactic acid, PLA is more hydrophobic than PGA. The hydrophobicity of high-molecular-weight PLA limits the water uptake of thin films to about 2% and results in a rate of backbone hydrolysis lower than that of PGA (Reed and Gilding, 1981). In addition, PLA is more soluble in organic solvents than is PGA.
\nIndustrial lactic acid production utilizes the lactic fermentation process rather than synthesis because the synthetic routes have many major limitations, including limited capacity due to the dependency on a by-product of another process, inability to only make the desirable L-lactic acid stereoisomer, and high manufacturing costs (Datta and Henry 2006). Three ways are possible for the polymerization of lactic acid (Jamshidian et al., 2010):
\nDirect condensation polymerization;
Direct poly-condensation in an azeotropic solution (an azeotrope is a mixture of 2 or more chemical liquids in such a ratio that its composition cannot be changed by simple distillation. This occurs because, when an azeotrope is boiled, the resulting vapor has the same ratio of constituents as the original mixture);
Polymerization through lactide formation.
Polymerization through lactide formation is being industrially accomplished for high molecular weight PLA production.
\nRecently, PLA, PGA, and their copolymers have been combined with bioactive ceramics such as Bioglass particles and hydroxyapatite that stimulate bone regeneration while greatly improving the mechanical strength of the composite material (Rezwan et al., 2006). Bioglass particles combined with D,L-PLA-
Polybutylene succinate (PBS), chemically synthesized by polycondensation of 1,4-butanedial with succinic acid (Fig. 14), is a chemosynthetic polyester with a relatively high melting temperature (Tm ~ 113°C) and favorable mechanical properties, which are comparable with those of such widely used polymers as polyethylene and polypropylene (Chen et al., 2011). PBS is thermoplastic, aliphatic polyester with many interesting properties including biodegradability, melt processability, and thermal and chemical resistance. In addition, its excellent processability in the field of both textiles into melt blown, multifilament, monofilament, nonwoven, flat, and split yarn fabrics and plastics into injection-molded products, makes it a promising polymer for various potential engineering applications (Lim et al., 2011). However, other properties of the PBS, such as its melt viscosity, melt strength, softness, and gas barrier characteristics are still regarded to be insufficient for various end-use applications.
\nPBS has a relatively low biodegradation rate because of its high crystallization rate and high crystallinity. To promote the physical properties, extend the application field, and increase the biodegradability of PBS, numerous approaches have been used, such as physical blending, copolymerization, or formation of composites (Okamoto et al., 2003).
\nOwing to the excellent processability of PBS, it can be processed using conventional polyolefin equipment in the range 160-200 ºC. Injection, extrusion or blow moulding is suitable for processing PBS. Its applications include mulch film, cutlery, containers, packaging film, bags and flushable hygiene products.
\nChemical structure of polybutylene succinate (
Because of the relatively low degradation rates, PBS can be copolymerized by adipate in order to increase the biodegradability. Poly (butylene succinate-
PBSA film has properties very similar to linear low-density polyethylene (LLDPE) and relatively high biodegradability, and is therefore suitable for a composting bag of kitchen waste (Ren et al., 2005).
\nChemical structure of polybutylene succinate adipate, where x = 4, y = 2, 4 (
Poly (vinyl alcohol) (PVOH) is the most readily biodegradable of vinyl polymers. It is readily degraded in waste-water-activated sludges. Unlike many vinyl polymers, PVOH is not prepared by polymerization of the corresponding monomer. The monomer, vinyl alcohol, almost exclusively exists as the tautomeric form, acetaldehyde. PVOH instead is prepared by partial or complete hydrolysis of polyvinyl acetate to remove acetate groups (Fig. 16). PVOH has a melting point of 180 to 190°C. It has a molecular weight of between 26,300 and 30,000, and a degree of hydrolysis of 86.5 to 89% (Ramaraj, 2007).
\nPVOH is an odorless and tasteless, translucent, white or cream colored granular powder. It is used as a moisture barrier film for food supplement tablets and for foods that contain inclusions or dry food with inclusions that need to be protected from moisture uptake. PVOH belongs to the water soluble polymers. In the context of the application, solubility and speed of solution are important characteristics.
\nPVOH has excellent film forming, emulsifying and adhesive properties. It is also resistant to oil, grease and solvents. It has high tensile strength and flexibility, as well as high oxygen and aroma barrier properties. However these properties are dependent on humidity, in other words, with higher humidity more water is absorbed. The water, which acts as a plasticizer, will then reduce its tensile strength, but increase its elongation and tear strength. PVOH is fully degradable and dissolves quickly (Vercauteren and Donners, 1986).
\nPVOH is the largest synthetic water-soluble polymer produced in the world. The prominent properties of PVOH may include its biodegradability in the environment. The generally accepted biodegradation mechanism occurs via a two-step reaction by oxidation of hydroxyl group followed by hydrolysis. The biodegradation of PVOH is influenced by the stereo-chemical configuration of the hydroxyl groups of PVOH. The isotactic material of PVOH preferentially degraded. The microbial degradation of PVOH has been studied, as well as its enzymatic degradation by secondary alcohol peroxidases isolated from soil bacteria of the
PVOH has been studied extensively because of its good biodegradability and mechanical properties. These properties have made PVOH as attractive material for disposable and biodegradable plastic substitutes. Its water solubility, reactivity, and biodegradability make it a potentially useful material in biomedical, agricultural, and water treatment areas, e.g. as a flocculant, metal-ion remover, and excipient for controlled release systems.
\nChemical structure of poly (vinyl alcohol), Where R= H or COCH3 (
Polyvinyl acetate, (PVA), is a rubbery synthetic polymer. It is a type of thermoplastic and belongs to the polyvinyl esters family. Polyvinyl acetate is prepared by polymerization of vinyl acetate monomer (free radical vinyl polymerization of the monomer vinyl acetate) (Fig. 17). The degree of polymerization of polyvinyl acetate typically is 100 to 5000. The ester groups of the polyvinyl acetate are sensitive to base hydrolysis and will slowly convert PVA into polyvinyl alcohol and acetic acid. Under alkaline conditions, boron compounds such as boric acid or borax cause the polymer to cross-link, forming tackifying precipitates or slime (Dionisio et al., 1993).
\nPVA reportedly undergoes biodegradation more slowly. Copolymers of ethylene and vinyl acetate were susceptible to slow degradation in soil-burial tests. The weight loss in a 120-day period increased with increasing acetate content. Because PVOH is obtained from the hydrolysis of PVA, which can be controlled easily in terms of the extent of hydrolysis and the sequence of PVA and PVOH, a controlled hydrolysis of PVA followed by controlled oxidation should provide degradation materials having a wide range of properties and degradability.
\nPolyvinyl acetate is a component of a widely used glue type, commonly referred to as wood glue, white glue, carpenter\'s glue, or PVA glue. The stiff homopolymer PVA, mostly the more soft copolymer a combination of vinyl acetate and ethylene, vinyl acetate ethylene (VAE), is used also in paper coatings, paint and other industrial coatings, as binder in nonwovens in glass fibers, sanitary napkins, filter paper and in textile finishing (Chandra and Rustgi, 1998).
\nChemical structure of poly (vinyl acetate) (
Several factors affect extent of polymer biodegradation that most impotents of them are polymer structure, polymer morphology, molecular weight, Radiation and chemical treatments.
\nNatural macromolecules, e.g. protein, cellulose, and starch are generally degraded in biological systems by hydrolysis followed by oxidation. It is not surprising, then, that most of the reported synthetic biodegradable polymers contain hydrolyzable linkages along the polymer chain; for example, amide enamine, ester, urea, and urethane linkages are susceptible to biodegradation by microorganisms and hydrolytic enzymes. Since many proteolytic enzymes specifically catalyze the hydrolysis of peptide linkages adjacent to substituents in proteins, substituted polymers containing substituents such as benzyl, hydroxy, carboxy, methyl, and phenyl groups have been prepared in the hope that an introduction of these substituents might increase biodegradability (Savenkova et al., 2000).
\nSince most enzyme-catalyzed reactions occur in aqueous media, the hydrophilic–hydrophobic character of synthetic polymers greatly affects their biodegradabilities. A polymer containing both hydrophobic and hydrophilic segments seems to have a higher biodegradability than those polymers containing either hydrophobic or hydrophilic structures only. A series of poly(alkylene tartrate)s was found to be readily assimilated by
However, the polymers derived from C6 and C8 alkane diols were more degradable than the more hydrophilic polymers derived from C2 and C4 alkane diols or the more hydrophobic polymers derived from the C10 and C12 alkane diols.
\nIn order for a synthetic polymer to be degradable by enzyme catalysis, the polymer chain must be flexible enough to fit into the active site of the enzyme. This most likely accounts for the fact that, whereas the flexible aliphatic polyesters are readily degraded by biological systems, the more rigid aromatic poly (ethylene terephthalate) is generally considered to be bioinert (Chandra and Rustgi, 1998).
\nOne of the principal differences between proteins and synthetic polymers is that proteins do not have equivalent repeating units along the polypeptide chains. This irregularity results in protein chains being less likely to crystallize. It is quite probable that this property contributes to the ready biodegradability of proteins. Synthetic polymers, on the other hand, generally have short repeating units, and this regularity enhances crystallization, making the hydrolyzable groups inaccessible to enzymes. It was reasoned that synthetic polymers with long repeating units would be less likely to crystallize and thus might be biodegradable; indeed, a series of poly (amide-urethane)s were found to be readily degraded by subtilisin (Zilberman et al., 2005).
\nSelective chemical degradation of semicrystalline polymer samples shows certain characteristic changes. During degradation, the crystallinity of the sample increases rapidly at first, then levels off to a much slower rate as the crystallinity approaches 100%. This is attributed to the eventual disappearance of the amorphous portions of the sample. The effect of morphology on the microbial and enzymatic degradation of PCL, a known biodegradable polymer with a number of potential applications, has been studied. Scanning electron microscopy (SEM) has shown that the degradation of a partially crystalline PCL film by filamentous fungi proceeds in a selective manner, with the amorphous regions being degraded prior to the degradation of the crystalline region. The microorganisms produce extracellular enzymes responsible for the selective degradation. This selectivity can be attributed to the less-ordered packing of amorphous regions, which permits easier access for the enzyme to the polymer chains. The size, shape and number of the crystallites all have a pronounced effect on the chain mobility of the amorphous regions and thus affect the rate of the degradation. This has been demonstrated by studying the effects of changing orientation via stretching on the degradation (Chandra and Rustgi, 1998).
\nBiodegradation proceeds differently from chemical degradation. Studies on the degradation by solutions of 40% aqueous methylamine have shown a difference in morphology and molecular weight changes and in the ability of the degrading agents to diffuse into the substrate. Also, it was found that the differences in degradation rates between amorphous and crystalline regions are not same. The enzyme is able to degrade the crystalline regions faster than can methylamine. Quantitative GPC (gel permeation chromatography) analysis shows that methylamines degrade the crystalline regions, forming single and double transverse length products. The enzyme system, on other hand, shows no intermediate molecular weight material and much smaller weight shift with degradation. This indicates that although degradation is selective, the crystalline portions are degraded shortly after the chain ends are made available to the exoenzyme. The lateral size of the crystallites has a strong effect on the rate of degradation because the edge of the crystal is where degradation of the crystalline material takes place, due to the crystal packing. A smaller lateral crystallite size yields a higher crystallite edge surface in the bulk polymer. Prior to the saturation of the enzyme active sites, the rate is dependent on available substrate; therefore, a smaller lateral crystallite size results in a higher rate of degradation. The degradation rate of a PCL film is zero order with respect to the total polymer, but is not zero order with respect to the concentrations of the crystallite edge material. The drawing of PCL films causes an increase in the rate of degradation, whereas annealing of the PCL causes a decrease in the rate of degradation. This is probably due to opposite changes in lateral crystallite sizes.
\n\n\n
Photolysis with UV light and the γ-ray irradiation of polymers generate radicals and/or ions that often lead to cleavage and crosslinking. Oxidation also occurs, complicating the situation, since exposure to light is seldom in the absence of oxygen. Generally this changes the material’s susceptibility to biodegradation. Initially, one expects the observed rate of degradation to increase until most of the fragmented polymer is consumed and a slower rate of degradation should follow for the crosslinked portion of the polymer. A study of the effects of UV irradiation on hydrolyzable polymers confirmed this. Similarly, photooxidation of polyalkenes promotes (slightly in most cases) the biodegradation. The formation of carbonyl and ester groups is responsible for this change (Miller and Williams, 1987).
\nProcesses have been developed to prepare copolymers of alkenes containing carbonyl groups so they will be more susceptible to photolytic cleavage prior to degradation. The problem with this approach is that negligible degradation was observed over a two year period for the buried specimens. Unless a prephotolysis arrangement can be made, the problem of plastic waste disposal remains serious, as it is undesirable to have open disposal, even with constant sunlight exposure.
\nAs expected, γ-ray irradiation greatly affects the rate of
There have been many studies on the effects of molecular weight on biodegradation processes. Most of the observed differences can be attributed to the limit of detecting the changes during degradation, or, even more often, the differences in morphology and hydrophilicity–hydrophobicity of polymer samples of varying molecular weight. Microorganisms produce both exoenzymes [degrading polymers from terminal groups (inwards)] and endoenzymes (degrading polymers randomly along the chain). One might expect a large molecular effect on the rate of degradation in the ease of exoenzymes and a relatively small molecular weight effect in the case of endoenzymes. Plastics remain relatively immune to microbial attack as long as their molecular weight remains high. Many plastics, such as poly ethylene, poly propylene and poly styrene do not support microbial growth. Low molecular weight hydrocarbons, however, can be degraded by microbes. They are taken in by microbial cells, ‘activated’ by attachment to coenzyme-A, and converted to cellular metabolites within the microbial cell. However, these processes do not function well (if at all) in an extracellular environment, and the plastic molecules are too large to enter the cell. This problem does not arise with natural molecules, such as starch and cellulose, because conversions to low molecular weight components by enzyme reactions occur outside the microbial cell. Photodegradation or chemical degradation may decrease molecular weight to the point that microbial attack can proceed, however (Chandra and Rustgi, 1998).
\nThe upper limits of molecular weight, beyond which uptake and intracellular degradation do not occur, have not been established for all alkane-derived materials. Very slow degradation of paraffins, PE glycols, and linear alkyl benzene sulphonates occurs when the length of the polymer chain exceeds 24–30 carbon atoms. It could be concluded from these amply documented results that alkane-based plastics with molecular weights exceeding 400–500 daltons (i.e. greater than 30 carbon atoms) must be degraded into smaller molecules by photodegradation, chemical or other biological means before biodegradation. LDPE with a molecular weight average of
Synthetic polymers are gradually being replaced by biodegradable materials especially those derived from replenishable, natural resources. Bioplastics development is just beginning; until now it covers approximately 5-10% of the current plastic market, about 50,000 t in Europe. More than the origin, the chemical structure of the biopolymer that determines its biodegradability. Use of such biopackagings will open up potential economic benefits to farmers and agricultural processors. The principal field regards the use of packaging films for food products, loose films used for transport packaging, service packaging like carry bags, cups, plates and cutlery, biowaste bags, in agricultural and horticultural fields like bags and compostable articles. Bilayer and multicomponent films resembling synthetic packaging materials with excellent barrier and mechanical properties need to be developed. Cross-linking, either chemically or enzymatically, of the various biomolecules is yet another approach of value in composite biodegradable films. Sustained multidisciplinary research efforts by chemists, polymer technologists, microbiologists, chemical engineers, environmental scientists and bureaucrats are needed for a successful implementation and commercialization of biopolymer-based eco-friendly packaging materials. Undoubtedly, biodegradation offers an attractive route to environmental waste management. Their development costs are high and yet they do not have the benefit of economic scale. It was shown that polyolefins present the same oxo-biodegradability of biopolymers, but they are more economical and effecting during use. Bio-based polymers have already found important applications in medicine field, where cost is much less important than function. It seems very unlikely that biodegradable oil based polymers will be displaced from their current role in packaging application, where cost is more important for the consumer market than environmental acceptability. Biopolymers fulfill the environmental concerns but they show some limitations in terms of performance like thermal resistance, barrier and mechanical properties, associated with the costs. Then, this kind of packaging materials needs more research, more added value like the introduction of smart and intelligent molecules (which is the nanotechnology field) able to give information about the properties of the material inside the package (quality, shelf-life, safety) and nutritional values. It is necessary to make researches on this kind of material to enhance barrier properties, to incorporate intelligent labelling, to give to the consumer the possibility to have more detailed product information than the current system.
\nIn a never-ending pursuit to understand individual criminality and how various social factors encourage or inhibit criminal activity, criminologists developed the concept of criminal careers [1, 2]. This concept emerged from the work of Alfred Blumstein [3], who aimed to quantify offending and improve the way criminology and other behavioural and social sciences make prediction and test empirical data. Using data from previous research, Blumstein et al. developed a concept of “criminal career” in order to develop a framework through which effective crime control policies could be developed [4]. Their understanding of the concept developed from indicating individual offending frequency (λ) calculated by determining the average number of crimes committed per year by active offenders (true frequency) measuring the individual arrest frequency (the average number of arrests per year of active offenders; measured frequency – the μ). They determined that λ and μ were linked by q, a probability of arrest following a crime (μ = λ ∗q) (μ = λ ∗q) [4].
At the same time, in the 1980s, the US showed an intense social and political focus on high crime rates and crime control. Due to the rise in violent crime, the US Government started a task force on violent crime which proposed increasing the federal role in the prosecution of violent crime, and other far-reaching changes in federal law including abolishing parole, expanding prisons, and restrictions on sentencing discretion [5]. To oppose these retributive measures, the National Research Council of the National Academy of Sciences organised a Panel in 1986, chaired by Alfred Blumstein, that focused on sharing knowledge and finding alternatives to this “tough on crime” policy [6].
The main focus of the Panel was to develop research on criminal careers, defined as “longitudinal sequence of offences committed by an individual” [3] and to distinguish “criminal careers” from a ‘career criminal’, i.e. “an extreme group of offenders who commit serious crimes at high rates over an extended period”. A criminal career is often considered a total number of crimes with regard to the duration of offending while career criminals term refers to persistent offenders. In other words, anyone can have a criminal career while only those with the highest frequency of offending can be described as career criminals [7]. The Panel was particularly interested in whether information about an individual’s criminal career (e.g. instant offence type, a record of prior offences, including any juvenile record, drug use) could help in determining criminal sanctions. Researchers on the Panel discussed the methodological, operational, and ethical challenges involved in using predictions about criminal careers in criminal justice decision making [5].
Central to the criminal career paradigm is that it recognises that individuals begin their criminal activity at some age, constitute a number of criminal offences, commit different types of criminal offences, and then desist or terminate offending. As a result, the approach to studying criminality through the concept of criminal careers emphasises the need to obtain data on why and when perpetrators start committing crimes, why they continue to offend, what is their frequency of offending, do they escalate and specialise in offending, and why and when they cease to offend [7].
Criminal career research has a somewhat complex organisation. It can be presented in several organising concepts that could be compared to a puzzle or branches on a tree [8]. At the beginning of the criminal career research, most researchers agreed that every criminal career has a beginning (“onset” or “initiation”), duration and end (“dropout”, “desistance” or “termination”) [9]. Additionally, criminal career concept is researched through four key dimensions - participation in offending, the individual offending frequency, the seriousness of the offences, and career length. These dimensions let to a set of related constructs and questions for researchers to explore [10]. The division into these features helps to understand, describe, and contextualise the offender’s criminal activity at a certain point in time [2].
Despite limitations and challenges, criminal career research shifted the focus from general to selective incapacitation strategies, trying to achieve the maximum possible crime reduction for the lowest possible cost [6]. Since its introduction to the criminological theoretical world, a significant amount of empirical, theoretical and policy-oriented research has been published [6] introducing new ways of looking at the crime phenomena such as age-crime curves [8], offending patterns, researching within-individual differences and differences between individuals [2, 6, 8]. Most importantly, investment in longitudinal research since the 1980s has contributed to creation of Developmental and Life-Course Criminology (DLC) in the 1990s [4]. Essentially, DLC is concerned with the development of offending, risk and protective factors and the effects of life events on the development of an individual by documenting and explaining within individual changes in offending throughout life [4, 8]. Although DLC theories resulted from research on criminal careers, criminal career was never established as a theory, rather as one of the paradigms of DLC.
Most of the criminal career research is focused on in-depth research of the criminological problems closely connected to onset, duration, and desistance from offending. In other words, criminal career research gravitated towards answering some important questions about general offending. However, little scholarly attention was given to sexual offences. Therefore, the field of sexual violence represent an important gateway to new insights and has much to offer to area of criminal career and life-course perspective.
In the past, sex offender literature has largely focused on biological and psychological roots of offending making individual pathologies and early trauma the most common explanations for sexual offending. Traditional theoretical views on sexual offending describe sexual offending through trait-like features such as cognitive distortions, low victim empathy, deviant sexual preferences, poor attachment style, sexual regulation. We will here briefly present some of the most used theoretical explanations regarding sex offending.
One of the major concept used in explanations of sexual offending is paraphilias who are defined as sexual disorders characterised by intense, sexually arousing fantasies involving either nonhuman objects, suffering or humiliation of oneself or one’s partner, children or other nonconsenting persons [11]. Most common paraphilias are paedophilia, voyeurism, transvestic disorder and exhibitionism [12]. However, it was found that unlike problems such as depression or anxiety, most cases of sexual offending (rape, child molestation, etc.) are not associated with any particular cluster of covarying signs and symptoms [13]. Moreover, even though some individuals who commit sexual offences against children are diagnosed with paedophilia, a mental disorder characterised by deviant sexual interests in children and some may have a paraphilia of some type, typically, this is not the case for all child sex offenders. [13]. Attachment theory, however, points out that sexual deviance is a consequence of the loss or emotional distress and intimacy deficits [14].
The psychodynamic theory explains sexual deviance as an expression of the unresolved problems experienced during the stages of development, and sexual deviancy occurs when the
Sex offending can also be studied through feminist theories focusing on the structure of the relationship between the sexes and the disparity of power between a man and a woman [16]. In general, feminist theories on child sexual abuse are divided into radical feminist perspective and post-modern perspective. Radical perspective centres on the patriarchal nature of (Western) society. Patriarchy has been defined by radical feminists as the world view that seeks to create and maintain male control over females. This perspective emphasises that sexual abuse is a representation of the patriarchy and the power that men have over women and that all men are socialised to hold attitude and exhibit behaviours that are associated with sexual offending. However, this perspective was criticised because of the lack of empirical scope [17]. The post-modern perspective rejected the radical perspective and established that men who sexually abuse are neither outside the society nor reducible to it and that they may know exactly what they are doing, but some offenders may not. This perspective was as well criticised because it is vague on what their theoretical position on sexual violence is [17].
Evolutionary theories, however, explain the diversity of human behaviours, including the aggression of sexual offenders as a way of adapting to changes in the environment. The evolutionary settings explain sexual aggression on sexual selection and reproductive strategies, whereby as one of the sexual strategies they cite the compulsion of sexual coercive is also present in the animal world as one of the reproductive strategies. The criminal offence of rape within these theories is a result of the inability of men to win a partner by more appropriate methods [16].
More influential theories in social sciences described sexual offending through cognition and behaviour. Behavioural theories suggest that deviant sexual behaviour is the result of learned behaviour. They assume that sexual excitement plays a crucial role in sexual offences. Sexual satisfaction and lack of negative consequences of sexually deviant behaviour increase the likelihood that such behaviour will continue. If the negative effects are strong enough, the behaviour will decrease [16]. However, many male sex offenders lack deviant sexual arousal patterns [18] and other traits such as the lack of empathy for the victim or remorse which in some individuals may also play a role in the development of deviant sexual behaviour patterns [16].
Cognitive theories suggest that cognitive distortions are the main cause of deviant sexual behaviour and that “concealed” opinion leads to “distorted” behaviour [16]. Cognitive distortions are often referred to as offence-supportive attitudes, or cognitive processing during an offence sequence, as well as post-hoc neutralisations or excuses for offending [19]. The cognitive-behavioural theory addresses how offenders’ thoughts affect their behaviour: focus on how sexual offenders diminish their feelings of guilt and shame by rationalising them through excuses and justification [14]. Although many offender rehabilitation programmes focus on eliminating cognitive distortions, the relationship between cognitive distortions and future crime is not clear and it seems cognitive distortions may not be what causes fallacious thinking at all because, outside the criminal context, “making excuses” for one’s behaviour is widely viewed as a normal thing [19].
Social learning theories explain how children who are victims of sexual abuse become perpetrators of sexual offences as adults. Also, this theory states that sexually explicit materials can contribute to committing sexual offences. Furthermore, victim’s age at the beginning of abuse, the relationship between the perpetrator and the victim, the type of sexual act, the amount of coercing used, and the duration and number of victimising events all have a crucial role in the connection of acts of sexual offences and subsequent acts of sexual delinquents [16]. This theory is most often criticised because there is little evidence to suggest that children who are victimised will become abusers themselves [20]. Furthermore, direct link between the use of pornography and sexual violence was never confirmed; however, some studies suggest there might be some connection between pornography and attitudes that support sexual aggression [16].
One of the limitations of these trait-like approaches is that it comes at a cost of a broader view of sexual offending. Moreover, individual pathologies-based explanations are not well suited for a developmental and longitudinal perspective of causes for this kind of behaviour. They have also contributed to sex offender researchers emphasising the differences, rather than searching for the similarities between sexual and nonsexual offending [6]. Criminal career approach provides a more developmental point of view explaining how sexual offending starts, develops, and stops as well as whether such distinctions are theoretically, clinically, and/or policy relevant [2]. In other words, maturation and dynamic theories assume that the same mechanisms underlie the behaviour of all offenders while life-course criminology emphasises the need to use a more broader view on the causes of sex offending as well as to find similarities between the perpetrators of these crimes rather than differences.
The most important theoretical question that needs to be answered when studying sex offenders through criminal careers is whether sex offenders should be regarded as similar or different from non-sexual offenders in terms of the aetiology of their offending behaviour. Consequently, there are two possible ways to consider sexual offending- that it is different from non-sexual offending and that it is an integral part of the general offending repertoire.
If studied separately, one possibility is to distinguish adolescence-limited from persistent sex offenders. This way of studying offenders gained its popularity in the 1990s with Moffit’s Dual Taxonomy theory [7]. This theory states that adolescence-limited antisocial behaviour individuals have brief criminal careers, lack consistency in their antisocial behaviour across situations and may also have irregular, crime-free periods. Adolescence-limited delinquents are likely to engage in antisocial behaviour in situations where they estimate they could gain profit, but they also abandon antisocial behaviour when prosocial behaviour is more rewarding.
Adolescence-limited offenders usually start offending by the ways of three distinctive conditions: motivation, social mimicry and reinforcement. Social mimicry is a term taken from the ethology and is loosely connected to the social mirroring theory. Adolescents often mimic the behaviour of their life-course-persistent peers in order to gain the same benefits (i.e., status in the peer groups; friends, etc.). In other words, adolescence-limited offenders commit criminal offences if they can gain from them, and if such behaviour is reinforced by their peers. With a change in their circumstances, for example, starting college or starting a job, they will usually stop offending [7].
Individuals that fall under the category of life-course-persistent antisocial behaviour exhibit antisocial behaviour at an early age. For example, biting and hitting at age 4, shoplifting and truancy at age 10, selling drugs and stealing cars at age 16, robbery and rape at age 22, and fraud and child abuse at age 30; the underlying disposition remains the same, but its expression changes form as new social opportunities arise at different points in development. The Dual taxonomy theory, however, does not suggest that all specific behaviours in childhood will be predictive of criminal behaviour in adulthood, but that it might be associated with behaviours that are conceptually consistent with the earlier behaviour [7].
Beyond young adulthood, the antisocial disposition of life-course-persistent may be expressed in a form that is not yet well-measured (e.g. neglect and abuse of family members). According to this theory, it is necessary to investigate the roots of antisocial behaviour in the early life of life-course-persistent offenders. Moreover, some of the causes for life-course-persistent offending are neuropsychological (disruption in the ontogenesis of the fetal brain, minor physical anomalies, maternal drug abuse, poor prenatal nutrition, exposure to toxic agents, brain injury because of complication during delivery), variability in infant temperament, developmental milestones and cognitive abilities, exposure to criminogenic environment or inconsistent discipline. Thus, over the years, an antisocial personality is slowly constructed. Likewise, deficits in language and reasoning are incrementally elaborated into academic failure and insufficient job skills. Over time, all the problems accumulate, and an individual has fewer options for change. This theory of life-course-persistent antisocial behaviour emphasises the constant process of reciprocal interaction between personality traits and environmental reactions to them [7].
However, if sex offending is studied combined, the question is to what extent sexual offending (or which type of sexual offending) is characteristic of a particular overall offending trajectory. Furthermore, it might be necessary to study sexual offenders by dividing them into different offence types as they may be stemming from different causal processes and thus it would be best to consider their different developmental pathways [6].
Current research suggests that there are some significant differences between sex offenders and non-sexual offenders. For example, the prevalence of general delinquency peaks in adolescence while sex offenders have two peaks in offending, one in adolescence and on in the mid to late 30s [6, 21, 22]. Also, sex offenders show continuity in general offending and antisocial behaviour, but low continuity in sexual offending [22]. This finding resonates with the finding that delinquency is usually just a part of a larger behavioural repertoire consisting of potentially harmful behaviours such as substance misuse [21]. Similarly, non-sexual offences usually precede sexual offences and specialisation in sex offending does not increase in adulthood [6, 22]. Therefore, criminal career offers an interesting way of studying offending patterns by looking into the four key dimensions (participation, frequency, seriousness, and length [3, 4, 9, 10, 23] and related constructs such as escalation, specialisation, co-offending, intermittency, and others [2, 9, 24, 25].
The dimension of participation refers to the proportion of a population who are active offenders at any given time, while frequency refers to the average annual rate at which this subgroup of active offenders commits crimes. In other words, participation refers to the prevalence of offending, while frequency refers to the incidence of crime [26]. Both dimensions are particularly difficult to research and estimate because of the underreporting of sexual offences and the so called “dark figures” of crime. Nevertheless, frequency has been of particular interest to the policymakers as it implicated that it is possible to identify high-frequency offenders and incapacitate them to maximise the incapacitation effect. However, it appears that the frequency of offending is very complex and that its value might fluctuate throughout a criminal career [27]. Another difficulty with the dimension of frequency is that it is challenging to estimate. For instance, one sex offender can offend against one victim over a, for example, 5-year period, multiple times, but official reports will note his offences as one offence.
The Cambridge study [28] found that the prevalence and frequency of sex offending in community samples is low and that that there is little evidence of recidivism or continuity. With respect to the prevalence and frequency of sex offending, conviction data indicated that whereas 41% of the males in the sample had been convicted by age 50, only 2.5% of the males had a conviction for a sex offence.
However, self-report and victimisation studies show a much higher number of individuals participating in sexual offending. For instance, The Bureau of Justice Statistics’ (BJS) in the US conducts a National Crime Victimisation Survey (NCVS) [29] every year using a nationally representative sample of 240,000 interviews on criminal victimisation. Results for 2018 showed that the rate of rape or sexual assault victimisation increased 1.6 to 2.7 victimizations per 1000 individuals aged 12 or older in comparison to previous years. Even more concerning data emerged from two meta-analyses [30, 31] of 282 studies around the world on child sexual abuse. The analyses used self-report data and found that the prevalence of child sexual abuse ranges from 12.7–19.7% for girls and 7.6–7.9% for boys. Both analyses showed that the highest prevalence of sexual abuse of children is in Africa and the lowest prevalence rate in Europe.
It is important to point out that sexual offences are often generalised as heterosexual offence [32] and therefore, there is very little known about male victims [33]. Recent figures indicate that around 12,000 males are victims of sexual assault or rape in the UK every year [34] and that nearly 1 in 4 men in the U.S. experienced some form of contact sexual violence in their lifetime. Among male victims of complete or attempted rape, about 71% experienced such victimisation before the age of 25 [35].
The frequency of offending varies significantly depending on the used method and between types of sex offenders. Sex offenders against women have, on average, 1.8 victims, whereas extra-familial child abusers have 3.4 victims on average [36]. Another study [37] suggests that sexual offenders with adult women victims had, on average, 2 official victims, but self-reported close to 12 victims. Similarly, sex offenders who offended against children had, on average, officially offended against 2 victims, but self-reported 7 victims.
Finally, investigations into the frequency of prolific, persistent sex offenders using several sources of information (i.e., self-reports, police investigations, victim statements) came up with three key conclusions on sex offending [38]. Firstly, about 11% of the sample committed over 300 sex crime events as opposed to about 40% who committed only one sex crime event. Secondly, some offenders take advantage of low-risk short-term opportunities with different victims, while others exploit a single offending opportunity by repeatedly offending against the same victim over a certain period of time. Thirdly, the findings revealed that the most prolific sex offenders were older, had a more conventional background characterised by a stable relationship with an adult partner, a job at the time of the offence (s), no drug issues, and no prior record for a sex crime.
The third dimension of the criminal career, seriousness, is in some ways, fundamental for criminal career research. Seriousness refers to the level of seriousness of the offences being committed by a given individual [3] and takes the modern approach to crime by focusing on determining a level of risk. Risk relates to the probability of criminal behaviour and is usually measured as low, moderate, or high [39, 40]. Offender risk is usually determined based on the possibility of reoffending or recidivism. It is important to define two categories here: chronic and recidivists [41]. The chronic type offenders are more at risk of persistent life-span criminal careers, which are costlier to society; over time they may have more victims (and financial loss for the victims) in addition to the costs associated with their arrest and incarceration. Recidivists are less persistent, as they have a lower incidence of reoffending. However, the categories do not differentiate on the severity of offences, only on offending timespan.
Offenders that maintain lengthier periods of offending tend to be generalists in their criminal behaviour [42, 43], their offences may be less severe than those that commit more severe types such as rape, sexual homicide, or child abuse. While there may be differences between types of reoffences (i.e. fraud and sex offences), society is risk-averse to more salient offences such as rape and child molestation [44, 45]. However, meta-analyses reports show that public fear of sexual offenders is often misplaced, and that offender’s recidivism rates for sexual offences vary between 11,5% and 13,7% while general recidivism rate varies between 33,2% and 36,2% [46, 47].
Some authors argue that these four key dimensions of criminal career, and especially seriousness, made it possible for a variety of important theoretical opportunities to emerge along with research on criminal careers [48]. This segment of criminal careers has several parameters that are observed through the dimension of seriousness: the severity of the criminal offence, escalation, specialisation, and crime-type switching [49].
The severity of the offence refers to the tendency towards committing severe criminal offences during a criminal career while escalation refers to the tendency towards making initial minor offences towards the increasingly more severe offences [27]. Escalation reflects the concern that as the career develops, the offender engages in more serious offences. There are different causes for escalation, for example, experience, increasing boldness or a stressful experience [6, 27].
Escalation research emerged interesting findings such as that most sex offenders who perform masturbation in front of their victim do not switch to a different sexual act (for example intercourse) when they abuse their second victim [50]. This finding points out that sex offenders tend to specialise in a particular sexual act and that they are not likely to escalate their sexual offending from non-contact to contact offending, for instance.
Another interesting finding [51] suggested that victim resistance plays a significant role in whether or not offenders will de-escalate in the amount of physical force used between victims. If the first victim resisted the offender, he was more likely to de-escalate in the level of physical force used. However, if the second victim resisted, the offender was less likely to de-escalate, indicating that he is adopting more force to secure subsequent victims if there is resistance. Furthermore, sex offenders who consume drugs in the hours before the first crime are more likely to de-escalate in the level of physical force used than be stable.
Crucial to determining behaviour consistency is the way an offender sources his victims. The “hunting” process is an essential stage of the sexual assault and offenders who find their victims through their relationships, or their occupation will often repeat their behaviour in their future crime. In contrast, offenders who find their victims more spontaneously are less likely to repeat this “hunting” method [52].
Specialisation is an aspect of criminal careers and is defined as likely repetitions of the same type of criminal offence [23] while versatility reflects a tendency to commit a broad array of offences without concentrating on a specific behaviour. Researchers use a “crime-switch” matrix, a matrix of crime types reflecting the probability that an offender who has previously committed one crime type will next commit a different type [27]. Research showed that there is a relationship between higher frequency of offending and versatility, but also that offenders who start offending at an early age tend to be involved in a diverse array of offences [6]. Most criminal careers are diverse, but there is some evidence of specialisation [21].
Sex offenders in specific show little empirical evidence of specialisation, but they are characterised by persistent criminal activity and tend to show criminal diversity in non-sexual crime types [53]. It was also found [54, 55, 56] that there are substantial differences between sex offenders regarding victim type. Sexual offenders against adults have more versatile criminal records [54, 55, 56], are more violent and have a higher frequency in offending [56] than abusers of children. The other group tends to be more specialised, have a higher frequency of sexual crimes, but also tend to be criminally versatile [45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56]. Extrafamilial child molesters are consistently found to specialise more in sexual offences [54, 55]. Overall, neither child abusers nor sex offenders against adult women have a specific type of victim and that the majority of both types typically commit more non-sexual offences than sexual [56].
The last dimension of criminal career research is the length; that is the time that an individual is actively offending. Research on the dimension of length attracted the attention of the scholarly community from the very beginning of criminal career research. It incorporates three head characteristics of criminal careers: beginning (“onset” or “initiation”), duration and end (“desistance” or “termination”) [2, 10].
The relationship between age and crime is of an asymmetrical bell shape (most commonly known as the “age-crime curve”), showing that the prevalence of offending tends to increase from late childhood, peaks in the teenage years (around ages 15–19), and then declines from the early 20s, often with a long tail [22]. Most offenders desist during late adolescence and early adulthood, irrespective of the age of onset [22], but an early age of onset appears to be connected to a relatively long criminal career [6, 50]. Research on recidivism shows that sexual offending decreases with age, as well as general offending, and the recidivism of sexual offenders that offend against adult decreases from early adulthood [57, 58]. However, unlike the general offender, sexual offenders usually start committing offences either in adolescence or in mid to late 30 [2, 59].
Some of the possible explanations of why sex offenders appear to be older when they start offending include that there is a high rate of unreported sex offences because the victims are less likely to report sexual offences than non-sexual offences and because there is a disbalance between official statistics and self-report. A gap between the age of onset in the official statistics and self-report is seven years [2]. Moreover, nearly 20% of sexual offenders are at the end of their criminal career, or their career has already desisted when they are convicted of their first sexual offence [2].
Alternatively, some authors [60] suggest that the discrepancy that goes against the logic of the life-course explanation of desistance could be explained by increased average ages of marriage and parenthood. In specific, the peak in sexual offending seems to occur at a time when adolescents already transitioned into adulthood which could mean that sex offenders do not transition into adulthood at the same pace as non-sexual offenders, or that these transitions do not affect recidivism in the same way as they do non-sexual offenders. Besides the transition into adulthood, several other reasons could influence individuals to start with sexual offending. For example, loneliness is commonly reported among sex offenders, that the transitions that influence common crime do not affect sexual offending (or affect it to a lesser extent), a cost-benefit analysis that makes sex offenders less likely to accept the cost of being labelled as a sex offender, conflicting interpersonal relationships, and maturation factors [60].
The above-described factors could affect the length of a criminal career, but the most significant variable affecting the length of a criminal career is the age of the first conviction. The likelihood of termination of offending is noticeably different as the age of the first conviction rises, and more severe offenders cease to offend after the first conviction only in fewer cases [61].
The dynamic concept of criminal careers empowered researchers to develop a whole new way of looking at crime. Traditional criminological theories offered a rather static explanation of one’s criminal conduct, while criminal career concept offered a view on criminal conduct that can be used to explain crime on an individual and group level. Furthermore, based on that, a new set of criminological theories emerged based on that concept, a Developmental and Life-Course Criminology, which emphasise a more dynamic approach to the aetiology and phenomenology of criminal conduct.
The main advantage of the criminal career concept regarding criminal conduct is that it offers a different perspective on criminality which can be used as a “bridge” between theoretical criminology and policy-relevant research. As we have shown on the example of perpetrators of sexual offences, there are differences in the development and peculiarities of the criminal career of these perpetrators. What the theories based on the concept of criminal career can offer are different explanations of the aetiology and phenomenology of these perpetrators about the particularities of their individual criminal careers. In this way, perpetrators can be viewed in terms of similarities and differences in the dimensions of a criminal career which can have multiple benefits. Specifically, although it is the same group of criminal offences, it is possible to have different theoretical explanations for the emergence and development of particular subgroups of perpetrators of certain criminal offences. In this way, specific prevention policies can be created that can be more effective because they identify not only the factors that influence the occurrence and development of certain types of crimes, but also the dynamics of the relationship between risk and protective factors that contribute to it. In this way, interventions at any level in terms of prevention and rehabilitation of offenders can be more successful.
Another great advantage of the concept of criminal career is that it allows the creation of new explanations of the origin and development of certain crimes, which contributes to the development and emergence of new criminological theories and concepts that, taking into account traditional explanations of crime and achievements of criminological research, could contribute to a new theory by erasing the understanding of committing crimes within the concepts of “traditional” and “newer” theories, but finding new ones that can merge all previous concepts into one that best suits the specifics of a particular crime or perpetrator.
It appears that only fractions of the concept and DLC theories are seldomly studied, causing the lack of comprehensive theory and resulting in some aspects of criminal careers not being researched enough while other areas are receiving more focus. This is true if we look at the types of crimes that are the focus of research under the concept of criminal career. The commission of sexual offences is now relatively unexplored and research to date has covered a small number of countries, mostly in the developed countries of the west. Therefore, the findings mentioned in the chapter need to be considered from the context of these countries. We hope that there is going to be more international collaboration and more scholarly focus on this topic in the years to come.
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Pedro",middleName:null,surname:"García Márquez",slug:"fausto-pedro-garcia-marquez",fullName:"Fausto Pedro García Márquez"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"10669",title:"Corrosion",subtitle:"Fundamentals and Protection Mechanisms",isOpenForSubmission:!1,hash:"4a76d54f8a40fc2e7002a8d13fd617c1",slug:"corrosion-fundamentals-and-protection-mechanisms",bookSignature:"Fahmina Zafar, Anujit Ghosal and Eram Sharmin",coverURL:"https://cdn.intechopen.com/books/images_new/10669.jpg",editedByType:"Edited by",publishedDate:"July 27th 2022",editors:[{id:"89672",title:"Dr.",name:"Fahmina",middleName:null,surname:"Zafar",slug:"fahmina-zafar",fullName:"Fahmina Zafar"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"10677",title:"Advanced Topics of Topology",subtitle:null,isOpenForSubmission:!1,hash:"bf964c52f9e653fac20a7fcab58070e5",slug:"advanced-topics-of-topology",bookSignature:"Francisco Bulnes",coverURL:"https://cdn.intechopen.com/books/images_new/10677.jpg",editedByType:"Edited by",publishedDate:"July 27th 2022",editors:[{id:"92918",title:"Dr.",name:"Francisco",middleName:null,surname:"Bulnes",slug:"francisco-bulnes",fullName:"Francisco Bulnes"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"11195",title:"Recent Advances in Biometrics",subtitle:null,isOpenForSubmission:!1,hash:"2d32e33e0f499cb5241734bb75dd2a83",slug:"recent-advances-in-biometrics",bookSignature:"Muhammad Sarfraz",coverURL:"https://cdn.intechopen.com/books/images_new/11195.jpg",editedByType:"Edited by",publishedDate:"July 27th 2022",editors:[{id:"215610",title:"Prof.",name:"Muhammad",middleName:null,surname:"Sarfraz",slug:"muhammad-sarfraz",fullName:"Muhammad Sarfraz"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}}]},subject:{topic:{id:"1165",title:"Urologic Oncology",slug:"urologic-oncology",parent:{id:"204",title:"Urology",slug:"urology"},numberOfBooks:4,numberOfSeries:0,numberOfAuthorsAndEditors:93,numberOfWosCitations:10,numberOfCrossrefCitations:3,numberOfDimensionsCitations:8,videoUrl:null,fallbackUrl:null,description:null},booksByTopicFilter:{topicId:"1165",sort:"-publishedDate",limit:12,offset:0},booksByTopicCollection:[{type:"book",id:"10339",title:"Modern Approach to Diagnosis and Treatment of Bladder Cancer",subtitle:null,isOpenForSubmission:!1,hash:"d1fdae263fb8a59eef2795dc748a1155",slug:"modern-approach-to-diagnosis-and-treatment-of-bladder-cancer",bookSignature:"Francesco Ziglioli and Umberto Maestroni",coverURL:"https://cdn.intechopen.com/books/images_new/10339.jpg",editedByType:"Edited by",editors:[{id:"62240",title:"Dr.",name:"Francesco",middleName:null,surname:"Ziglioli",slug:"francesco-ziglioli",fullName:"Francesco Ziglioli"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"6424",title:"Evolving Trends in Kidney Cancer",subtitle:null,isOpenForSubmission:!1,hash:"e9305ef1c5e6ad63407fd9262a27cf31",slug:"evolving-trends-in-kidney-cancer",bookSignature:"Sashi S. Kommu and Inderbir S. Gill",coverURL:"https://cdn.intechopen.com/books/images_new/6424.jpg",editedByType:"Edited by",editors:[{id:"9902",title:"Dr.",name:"Sashi S.",middleName:"S",surname:"Kommu",slug:"sashi-s.-kommu",fullName:"Sashi S. Kommu"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"6423",title:"Prostate Cancer",subtitle:null,isOpenForSubmission:!1,hash:"d072a079624084c12169a118fdbbfa87",slug:"prostate-cancer",bookSignature:"Cem Onal",coverURL:"https://cdn.intechopen.com/books/images_new/6423.jpg",editedByType:"Edited by",editors:[{id:"43940",title:"Dr.",name:"Cem",middleName:null,surname:"Onal",slug:"cem-onal",fullName:"Cem Onal"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"5516",title:"Bladder Cancer",subtitle:"Management of NMI and Muscle-Invasive Cancer",isOpenForSubmission:!1,hash:"fa255c022acc85f2bd2c12ce4cd9a67b",slug:"bladder-cancer-management-of-nmi-and-muscle-invasive-cancer",bookSignature:"M. Hammad Ather",coverURL:"https://cdn.intechopen.com/books/images_new/5516.jpg",editedByType:"Edited by",editors:[{id:"88868",title:"Prof.",name:"M Hammad",middleName:null,surname:"Ather",slug:"m-hammad-ather",fullName:"M Hammad Ather"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}}],booksByTopicTotal:4,seriesByTopicCollection:[],seriesByTopicTotal:0,mostCitedChapters:[{id:"54132",doi:"10.5772/67473",title:"Cross-Polarization OCT for In Vivo Diagnostics and Prediction of Bladder Cancer",slug:"cross-polarization-oct-for-in-vivo-diagnostics-and-prediction-of-bladder-cancer",totalDownloads:1119,totalCrossrefCites:1,totalDimensionsCites:3,abstract:"This chapter contains three parts covering recent efforts to increase the accuracy of optical coherence tomography (OCT) differential diagnostics of bladder pathologies. The first part compares the diagnostic efficacy of traditional OCT and cross-polarization OCT (CP OCT); CP OCT and fluorescence cystoscopy (FC) for detecting flat lesions in the bladder at the early stages of cancer. The second part contains a report on achievements in application of CP OCT for detection of recurrent carcinoma in the scar area that is a hardly distinguishable form of bladder cancer using an optimized CP OCT image analysis. The third part of the chapter reviews the results on CP OCT usage for in vivo diagnosis of the bladder cancer after radiation therapy of cervical cancer.",book:{id:"5516",slug:"bladder-cancer-management-of-nmi-and-muscle-invasive-cancer",title:"Bladder Cancer",fullTitle:"Bladder Cancer - Management of NMI and Muscle-Invasive Cancer"},signatures:"Elena Kiseleva, Gladkova Natalia, Streltzova Olga, Kirillin Mikhail,\nMaslennikova Anna, Dudenkova Varvara, Yunusova Katerina and\nSergeeva Ekaterina",authors:[{id:"68196",title:"Prof.",name:"Natalia",middleName:null,surname:"Gladkova",slug:"natalia-gladkova",fullName:"Natalia Gladkova"},{id:"191970",title:"Dr.",name:"Elena",middleName:null,surname:"Kiseleva",slug:"elena-kiseleva",fullName:"Elena Kiseleva"},{id:"191990",title:"Dr.",name:"Olga",middleName:null,surname:"Streltzova",slug:"olga-streltzova",fullName:"Olga Streltzova"},{id:"191992",title:"Mrs.",name:"Varvara",middleName:null,surname:"Dudenkova",slug:"varvara-dudenkova",fullName:"Varvara Dudenkova"},{id:"191993",title:"Prof.",name:"Anna",middleName:null,surname:"Maslennikova",slug:"anna-maslennikova",fullName:"Anna Maslennikova"},{id:"191994",title:"Dr.",name:"Katerina",middleName:null,surname:"Yunusova",slug:"katerina-yunusova",fullName:"Katerina Yunusova"},{id:"191995",title:"Dr.",name:"Mikhail",middleName:null,surname:"Kirillin",slug:"mikhail-kirillin",fullName:"Mikhail Kirillin"},{id:"193422",title:"Dr.",name:"Ekaterina",middleName:null,surname:"Sergeeva",slug:"ekaterina-sergeeva",fullName:"Ekaterina Sergeeva"}]},{id:"54019",doi:"10.5772/67309",title:"Bladder Cancer Markers and Recent Innovations",slug:"bladder-cancer-markers-and-recent-innovations",totalDownloads:1702,totalCrossrefCites:1,totalDimensionsCites:1,abstract:"Bladder cancer (urothelial carcinoma) is the most common tumor of the urinary tract. It occurs more frequently among men about 65 years old on average. Two forms of the tumor are known: a non–muscle-invasive one and a muscle-invasive one. The latter turns out to be very aggressive with a survival of 5 years average. The non–muscle-invasive form frequently recurs (60–70%) and in 15% of cases, it progresses into the invasive form. The diagnosis is made mainly by cystoscopy and urine cytology. A high number of researches were dedicated in order to find a simple test using voided urine to frequently monitor possible tumor recurrence. During the last 10 years, many tests were proposed concerning either special proteins of which the most common are the bladder tumor antigen (BTA) and the nuclear matrix protein 22 (NMP22) or the presence of genetic mutations [most frequently, fibroblasts growth factor receptor 3 (FGFR3) and TP53], alteration of DNA methylation, chromatin structure and, more recently, the presence of specific micro-RNA. Recently the analysis of lipids present in voided urine showed a difference in fatty acids between healthy individuals and those affected by non-invasive forms. These markers appear to have a high specificity and sensitivity: a deepening of these results could lead to the development of a test that avoids invasive treatment and the cost of cystoscopy.",book:{id:"5516",slug:"bladder-cancer-management-of-nmi-and-muscle-invasive-cancer",title:"Bladder Cancer",fullTitle:"Bladder Cancer - Management of NMI and Muscle-Invasive Cancer"},signatures:"Mariapia Viola-Magni, Samuela Cataldi and Daniela Marocco",authors:[{id:"192375",title:"Prof.",name:"Mariapia",middleName:null,surname:"Viola-Magni",slug:"mariapia-viola-magni",fullName:"Mariapia Viola-Magni"},{id:"197851",title:"BSc.",name:"Samuela",middleName:null,surname:"Cataldi",slug:"samuela-cataldi",fullName:"Samuela Cataldi"},{id:"197852",title:"Dr.",name:"Daniela",middleName:null,surname:"Marocco",slug:"daniela-marocco",fullName:"Daniela Marocco"}]},{id:"54063",doi:"10.5772/67280",title:"Intravesical Chemohyperthermia for NMIBC: Rationale and Results of This Developing Treatment",slug:"intravesical-chemohyperthermia-for-nmibc-rationale-and-results-of-this-developing-treatment",totalDownloads:1395,totalCrossrefCites:0,totalDimensionsCites:1,abstract:"Bladder cancer is the fourth most common cancer in men, and the lifetime risk of getting bladder cancer is 2.4%. Approximately 75% of newly diagnosed cases of bladder cancer are non-muscle-invasive bladder cancer (NMIBC), and half of them will show recurrence and/or progression after transurethral resection. Therefore, after transurethral resection, in high-risk patients, intravesical therapy is mandatory. However, bacillus Calmette-Guérin (BCG) is associated with important side effects such as systemic tuberculosis and bladder retraction. Chemohyperthermia (CHT) has shown a 60% lower recurrence rate than standard mitomycin C (MMC). However, its effectiveness in high-risk patients, especially CIS and BCG refractory patients, is even more important. CHT will probably be an option for patients unsuitable for radical cystectomy or those on whom BCG can’t be used. Two main technologies are currently available for intravesical CHT: microwaves and recirculating heated fluids. Both of them have pros and cons that should be known and evaluated by a urologist. In this chapter, we will speak about rationale, technical options, clinical results, ongoing studies, and future perspective for this interesting treatment option for intermediate and high-risk patients with NMIBC.",book:{id:"5516",slug:"bladder-cancer-management-of-nmi-and-muscle-invasive-cancer",title:"Bladder Cancer",fullTitle:"Bladder Cancer - Management of NMI and Muscle-Invasive Cancer"},signatures:"Sousa-Escandón Manuel Alejandro, Flores Carbajal Javier, Sousa-\nGonzález Daniel and Rodriguez Gómez Silvia",authors:[{id:"191356",title:"Dr.",name:"Alejandro",middleName:null,surname:"Sousa-Escandón",slug:"alejandro-sousa-escandon",fullName:"Alejandro Sousa-Escandón"}]},{id:"54147",doi:"10.5772/67443",title:"Lymphadenectomy in Muscle Invasive Bladder Cancer",slug:"lymphadenectomy-in-muscle-invasive-bladder-cancer",totalDownloads:1273,totalCrossrefCites:0,totalDimensionsCites:1,abstract:"Bladder cancer is the second most common genitourinary malignancy with urothelial cancer comprising nearly 90% of primary bladder tumors. Urothelial carcinoma of the urinary bladder is the fifth most common malignancy in the United States, with an estimated 76,960 new cases and 163,900 deaths in 2016. Radical cystectomy with lymph node dissection remains the standard treatment for patients with muscle-invasive urothelial carcinoma of the bladder, and also for nonmuscle-invasive disease, refractory to intravesical therapy. The current approaches to pelvic lymph node dissections are based on the removal of lymph nodes most commonly harboring metastatic disease, notably the external iliac, obturator, and hypogastric lymph nodes. The boundaries for a standard pelvic lymph node dissection generally include the bifurcation of the common iliac vessels superiorly and the genitofemoral nerve laterally. Extended pelvic lymph node includes the removal of lymph nodes between the bifurcation of the common iliac vessels and the level of the aortic bifurcation, sometimes including distal aortic and caval nodes up to the level of the inferior mesenteric artery, as well as presacral nodes. Extended and superextended dissection has been reported to be associated with superior survival outcome.",book:{id:"5516",slug:"bladder-cancer-management-of-nmi-and-muscle-invasive-cancer",title:"Bladder Cancer",fullTitle:"Bladder Cancer - Management of NMI and Muscle-Invasive Cancer"},signatures:"Mustafa Ozan Horsanali and Kutan Ozer",authors:[{id:"59702",title:"Dr.",name:"Mustafa Ozan",middleName:null,surname:"Horsanali",slug:"mustafa-ozan-horsanali",fullName:"Mustafa Ozan Horsanali"},{id:"192699",title:"Dr.",name:"Kutan",middleName:null,surname:"Ozer",slug:"kutan-ozer",fullName:"Kutan Ozer"}]},{id:"59222",doi:"10.5772/intechopen.73515",title:"Development of Oncolytic Adenoviruses for the Management of Prostate Cancer",slug:"development-of-oncolytic-adenoviruses-for-the-management-of-prostate-cancer",totalDownloads:1121,totalCrossrefCites:1,totalDimensionsCites:1,abstract:"Prostate cancer (PCa) is the fifth most common cause of cancer-related deaths in men globally. Androgen receptor (AR) signalling plays a vital role in initiation and progression and antiandrogens are standard of care first-line therapeutics. However, resistance frequently develops resulting in metastatic castration-resistant prostate cancer (mCRPC). Management of CRPC is currently chemotherapy and/or radiotherapy but is mostly palliative due to rapid development of resistance. The need for novel approaches to eliminate mCRPC is compelling; a promising option is replication-selective (oncolytic) adenoviruses with demonstrated efficacy in preclinical models of multidrug-resistant PCa. The safety of various viral mutants has been confirmed in numerous clinical trials with minimal toxicity in patients. Importantly, oncolytic adenoviruses synergise with the current standard of care for mCRPC even in treatment-resistant cells. In early phase I–II clinical trials, promising efficacy in patients with localised PCa was reported after intratumoural administration, and phase III trials are underway. To enable systemic delivery, for targeting of mCRPC, further developments are necessary because of the short half-life of the adenoviral mutants in human blood. Current progress in preventing the high-affinity binding of adenovirus to erythrocytes, hepatocyte uptake, and elimination by hepatic Kupffer cells will be described.",book:{id:"6423",slug:"prostate-cancer",title:"Prostate Cancer",fullTitle:"Prostate Cancer"},signatures:"Ahmed A. Ali and Gunnel Halldén",authors:[{id:"80427",title:"Dr.",name:"Gunnel",middleName:null,surname:"Hallden",slug:"gunnel-hallden",fullName:"Gunnel Hallden"},{id:"232386",title:"MSc.",name:"Ahmed",middleName:null,surname:"Ali",slug:"ahmed-ali",fullName:"Ahmed Ali"}]}],mostDownloadedChaptersLast30Days:[{id:"70881",title:"Robot-Assisted Partial Nephrectomy: Evolving Techniques",slug:"robot-assisted-partial-nephrectomy-evolving-techniques",totalDownloads:494,totalCrossrefCites:0,totalDimensionsCites:0,abstract:"Robotic-assisted partial nephrectomy is now embraced in urology as a recommended treatment option for small localised renal tumours. There is an increasing trend towards setting up robotic-assisted services in urological centres across the world. Our aim is to review the available published common robotic-assisted partial nephrectomy techniques. We present our institutions’ established step-by-step technique for performing robotic-assisted partial nephrectomy, in order to guide aspiring urologists interested in performing robotic-assisted partial nephrectomies. The importance of pre-operative review of imaging in a multi-disciplinary approach is critical. We emphasise certain tips inperforming a safer procedure.",book:{id:"6424",slug:"evolving-trends-in-kidney-cancer",title:"Evolving Trends in Kidney Cancer",fullTitle:"Evolving Trends in Kidney Cancer"},signatures:"Mohammed Kamil Quraishi, Edward Ramez Latif, Milan Thomas, Ben Eddy, Elio Mazzone and Alexandre Mottrie",authors:[{id:"277566",title:"Dr.",name:"Mohammed Kamil",middleName:null,surname:"Quraishi",slug:"mohammed-kamil-quraishi",fullName:"Mohammed Kamil Quraishi"},{id:"277570",title:"Dr.",name:"Milan",middleName:null,surname:"Thomas",slug:"milan-thomas",fullName:"Milan Thomas"},{id:"277571",title:"Dr.",name:"Ben",middleName:null,surname:"Eddy",slug:"ben-eddy",fullName:"Ben Eddy"}]},{id:"54132",title:"Cross-Polarization OCT for In Vivo Diagnostics and Prediction of Bladder Cancer",slug:"cross-polarization-oct-for-in-vivo-diagnostics-and-prediction-of-bladder-cancer",totalDownloads:1118,totalCrossrefCites:1,totalDimensionsCites:3,abstract:"This chapter contains three parts covering recent efforts to increase the accuracy of optical coherence tomography (OCT) differential diagnostics of bladder pathologies. The first part compares the diagnostic efficacy of traditional OCT and cross-polarization OCT (CP OCT); CP OCT and fluorescence cystoscopy (FC) for detecting flat lesions in the bladder at the early stages of cancer. The second part contains a report on achievements in application of CP OCT for detection of recurrent carcinoma in the scar area that is a hardly distinguishable form of bladder cancer using an optimized CP OCT image analysis. The third part of the chapter reviews the results on CP OCT usage for in vivo diagnosis of the bladder cancer after radiation therapy of cervical cancer.",book:{id:"5516",slug:"bladder-cancer-management-of-nmi-and-muscle-invasive-cancer",title:"Bladder Cancer",fullTitle:"Bladder Cancer - Management of NMI and Muscle-Invasive Cancer"},signatures:"Elena Kiseleva, Gladkova Natalia, Streltzova Olga, Kirillin Mikhail,\nMaslennikova Anna, Dudenkova Varvara, Yunusova Katerina and\nSergeeva Ekaterina",authors:[{id:"68196",title:"Prof.",name:"Natalia",middleName:null,surname:"Gladkova",slug:"natalia-gladkova",fullName:"Natalia Gladkova"},{id:"191970",title:"Dr.",name:"Elena",middleName:null,surname:"Kiseleva",slug:"elena-kiseleva",fullName:"Elena Kiseleva"},{id:"191990",title:"Dr.",name:"Olga",middleName:null,surname:"Streltzova",slug:"olga-streltzova",fullName:"Olga Streltzova"},{id:"191992",title:"Mrs.",name:"Varvara",middleName:null,surname:"Dudenkova",slug:"varvara-dudenkova",fullName:"Varvara Dudenkova"},{id:"191993",title:"Prof.",name:"Anna",middleName:null,surname:"Maslennikova",slug:"anna-maslennikova",fullName:"Anna Maslennikova"},{id:"191994",title:"Dr.",name:"Katerina",middleName:null,surname:"Yunusova",slug:"katerina-yunusova",fullName:"Katerina Yunusova"},{id:"191995",title:"Dr.",name:"Mikhail",middleName:null,surname:"Kirillin",slug:"mikhail-kirillin",fullName:"Mikhail Kirillin"},{id:"193422",title:"Dr.",name:"Ekaterina",middleName:null,surname:"Sergeeva",slug:"ekaterina-sergeeva",fullName:"Ekaterina Sergeeva"}]},{id:"61307",title:"Genetics in the Prostate Cancer",slug:"genetics-in-the-prostate-cancer",totalDownloads:1138,totalCrossrefCites:0,totalDimensionsCites:0,abstract:"Any disruption in the intracellular functions ranging from DNA transcription to protein ligand binding as well as intercellular communication may cause cellular transformation to malignant cell in the proper microenvironment when it could escape from the immune system. In this chapter, specifically, genetic alterations playing role in the prostate cancer are intended to be reviewed briefly under the subheadings of genomic instability and the hallmarks of cancer which are sustaining proliferative signaling, evading growth suppressors, resisting cell death, enabling the replicative immortality, inducing angiogenesis, activating invasion and progression to metastatic disease, reprogramming of the energy metabolism and evading immune destruction.",book:{id:"6423",slug:"prostate-cancer",title:"Prostate Cancer",fullTitle:"Prostate Cancer"},signatures:"Hikmet Köseoğlu",authors:[{id:"111496",title:"Dr.",name:"Hikmet",middleName:null,surname:"Köseoǧlu",slug:"hikmet-koseolu",fullName:"Hikmet Köseoǧlu"}]},{id:"54587",title:"Genital Organs‐Sparing Radical Cystectomy in Female Patients with Muscle Invasive Urothelial Carcinoma of the Bladder",slug:"genital-organs-sparing-radical-cystectomy-in-female-patients-with-muscle-invasive-urothelial-carcino",totalDownloads:1296,totalCrossrefCites:0,totalDimensionsCites:0,abstract:"There has been considerable interest in urethral‐sparing cystectomy and preservation of the gynecological tract to maintain continence mechanism, sexual function, and reproductive function in young patients who undergo radical cystectomy for muscle‐invasive bladder cancer and this new technique gained acceptance in many centers. The issue of oncological safety of a urethra and anterior vaginal wall‐sparing cystectomy in selected patients has been addressed by several authors. The chapter will discuss the following items: (I) Technique of genital‐sparing radical cystectomy in female patients with muscle invasive transitional cell carcinoma of the bladder. (II) Definition and rationale of genital‐sparing radical cystectomy in female patients. (III) Rational and value of urethral preservation in genital‐sparing cystectomy in female patients with urothelial carcinoma. (IV) Previous reports about genital‐sparing cystectomy in patients with urothelial carcinoma. (V) Value of preservation of the internal genital organs in female patients undergoing radical cystectomy.",book:{id:"5516",slug:"bladder-cancer-management-of-nmi-and-muscle-invasive-cancer",title:"Bladder Cancer",fullTitle:"Bladder Cancer - Management of NMI and Muscle-Invasive Cancer"},signatures:"Hosni Khairy Salem",authors:[{id:"96052",title:"Prof.",name:"Hosni",middleName:"Khairy",surname:"Salem",slug:"hosni-salem",fullName:"Hosni Salem"}]},{id:"67209",title:"Robotic Surgery and Successful Set-Up: A Stepwise Approach",slug:"robotic-surgery-and-successful-set-up-a-stepwise-approach",totalDownloads:690,totalCrossrefCites:0,totalDimensionsCites:0,abstract:"Robot purchase, implementation, and sustainability require a number of key challenges to overcome. We provide our experience of managing a potentially daunting task, summarizing the key steps to help deliver such an exciting project. We will take you through team approach options for purchase and safe implementation in the current financial climate.",book:{id:"6424",slug:"evolving-trends-in-kidney-cancer",title:"Evolving Trends in Kidney Cancer",fullTitle:"Evolving Trends in Kidney Cancer"},signatures:"Christopher J. Anderson and Hiten R.H. 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He also obtained an MSc in Molecular and Genetic Medicine, and a Ph.D. in Clinical Immunology and Human Genetics from the University of Sheffield, UK. He also completed a short-term fellowship in Pediatric Clinical Immunology and Bone Marrow Transplantation at Newcastle General Hospital, England. Dr. Rezaei is a Full Professor of Immunology and Vice Dean of International Affairs and Research, at the School of Medicine, Tehran University of Medical Sciences, and the co-founder and head of the Research Center for Immunodeficiencies. He is also the founding president of the Universal Scientific Education and Research Network (USERN). Dr. Rezaei has directed more than 100 research projects and has designed and participated in several international collaborative projects. He is an editor, editorial assistant, or editorial board member of more than forty international journals. He has edited more than 50 international books, presented more than 500 lectures/posters in congresses/meetings, and published more than 1,100 scientific papers in international journals.",institutionString:"Tehran University of Medical Sciences",institution:{name:"Tehran University of Medical Sciences",country:{name:"Iran"}}},{id:"180733",title:"Dr.",name:"Jean",middleName:null,surname:"Engohang-Ndong",slug:"jean-engohang-ndong",fullName:"Jean Engohang-Ndong",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/180733/images/system/180733.png",biography:"Dr. Jean Engohang-Ndong was born and raised in Gabon. After obtaining his Associate Degree of Science at the University of Science and Technology of Masuku, Gabon, he continued his education in France where he obtained his BS, MS, and Ph.D. in Medical Microbiology. He worked as a post-doctoral fellow at the Public Health Research Institute (PHRI), Newark, NJ for four years before accepting a three-year faculty position at Brigham Young University-Hawaii. Dr. Engohang-Ndong is a tenured faculty member with the academic rank of Full Professor at Kent State University, Ohio, where he teaches a wide range of biological science courses and pursues his research in medical and environmental microbiology. Recently, he expanded his research interest to epidemiology and biostatistics of chronic diseases in Gabon.",institutionString:"Kent State University",institution:{name:"Kent State University",country:{name:"United States of America"}}},{id:"188773",title:"Prof.",name:"Emmanuel",middleName:null,surname:"Drouet",slug:"emmanuel-drouet",fullName:"Emmanuel Drouet",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/188773/images/system/188773.png",biography:"Emmanuel Drouet, PharmD, is a Professor of Virology at the Faculty of Pharmacy, the University Grenoble-Alpes, France. As a head scientist at the Institute of Structural Biology in Grenoble, Dr. Drouet’s research investigates persisting viruses in humans (RNA and DNA viruses) and the balance with our host immune system. He focuses on these viruses’ effects on humans (both their impact on pathology and their symbiotic relationships in humans). He has an excellent track record in the herpesvirus field, and his group is engaged in clinical research in the field of Epstein-Barr virus diseases. He is the editor of the online Encyclopedia of Environment and he coordinates the Universal Health Coverage education program for the BioHealth Computing Schools of the European Institute of Science.",institutionString:null,institution:{name:"Grenoble Alpes University",country:{name:"France"}}},{id:"131400",title:"Prof.",name:"Alfonso J.",middleName:null,surname:"Rodriguez-Morales",slug:"alfonso-j.-rodriguez-morales",fullName:"Alfonso J. Rodriguez-Morales",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/131400/images/system/131400.png",biography:"Dr. Rodriguez-Morales is an expert in tropical and emerging diseases, particularly zoonotic and vector-borne diseases (especially arboviral diseases). He is the president of the Travel Medicine Committee of the Pan-American Infectious Diseases Association (API), as well as the president of the Colombian Association of Infectious Diseases (ACIN). He is a member of the Committee on Tropical Medicine, Zoonoses, and Travel Medicine of ACIN. He is a vice-president of the Latin American Society for Travel Medicine (SLAMVI) and a Member of the Council of the International Society for Infectious Diseases (ISID). Since 2014, he has been recognized as a Senior Researcher, at the Ministry of Science of Colombia. He is a professor at the Faculty of Medicine of the Fundacion Universitaria Autonoma de las Americas, in Pereira, Risaralda, Colombia. He is an External Professor, Master in Research on Tropical Medicine and International Health, Universitat de Barcelona, Spain. He is also a professor at the Master in Clinical Epidemiology and Biostatistics, Universidad Científica del Sur, Lima, Peru. In 2021 he has been awarded the “Raul Isturiz Award” Medal of the API. Also, in 2021, he was awarded with the “Jose Felix Patiño” Asclepius Staff Medal of the Colombian Medical College, due to his scientific contributions to COVID-19 during the pandemic. He is currently the Editor in Chief of the journal Travel Medicine and Infectious Diseases. His Scopus H index is 47 (Google Scholar H index, 68).",institutionString:"Institución Universitaria Visión de las Américas, Colombia",institution:null},{id:"332819",title:"Dr.",name:"Chukwudi Michael",middleName:"Michael",surname:"Egbuche",slug:"chukwudi-michael-egbuche",fullName:"Chukwudi Michael Egbuche",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/332819/images/14624_n.jpg",biography:"I an Dr. Chukwudi Michael Egbuche. I am a Senior Lecturer in the Department of Parasitology and Entomology, Nnamdi Azikiwe University, Awka.",institutionString:null,institution:{name:"Nnamdi Azikiwe University",country:{name:"Nigeria"}}},{id:"284232",title:"Mr.",name:"Nikunj",middleName:"U",surname:"Tandel",slug:"nikunj-tandel",fullName:"Nikunj Tandel",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/284232/images/8275_n.jpg",biography:'Mr. Nikunj Tandel has completed his Master\'s degree in Biotechnology from VIT University, India in the year of 2012. He is having 8 years of research experience especially in the field of malaria epidemiology, immunology, and nanoparticle-based drug delivery system against the infectious diseases, autoimmune disorders and cancer. He has worked for the NIH funded-International Center of Excellence in Malaria Research project "Center for the study of complex malaria in India (CSCMi)" in collaboration with New York University. The preliminary objectives of the study are to understand and develop the evidence-based tools and interventions for the control and prevention of malaria in different sites of the INDIA. Alongside, with the help of next-generation genomics study, the team has studied the antimalarial drug resistance in India. Further, he has extended his research in the development of Humanized mice for the study of liver-stage malaria and identification of molecular marker(s) for the Artemisinin resistance. At present, his research focuses on understanding the role of B cells in the activation of CD8+ T cells in malaria. Received the CSIR-SRF (Senior Research Fellow) award-2018, FIMSA (Federation of Immunological Societies of Asia-Oceania) Travel Bursary award to attend the IUIS-IIS-FIMSA Immunology course-2019',institutionString:"Nirma University",institution:{name:"Nirma University",country:{name:"India"}}},{id:"334383",title:"Ph.D.",name:"Simone",middleName:"Ulrich",surname:"Ulrich Picoli",slug:"simone-ulrich-picoli",fullName:"Simone Ulrich Picoli",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/334383/images/15919_n.jpg",biography:"Graduated in Pharmacy from Universidade Luterana do Brasil (1999), Master in Agricultural and Environmental Microbiology from Federal University of Rio Grande do Sul (2002), Specialization in Clinical Microbiology from Universidade de São Paulo, USP (2007) and PhD in Sciences in Gastroenterology and Hepatology (2012). She is currently an Adjunct Professor at Feevale University in Medicine and Biomedicine courses and a permanent professor of the Academic Master\\'s Degree in Virology. She has experience in the field of Microbiology, with an emphasis on Bacteriology, working mainly on the following topics: bacteriophages, bacterial resistance, clinical microbiology and food microbiology.",institutionString:null,institution:{name:"Universidade Feevale",country:{name:"Brazil"}}},{id:"229220",title:"Dr.",name:"Amjad",middleName:"Islam",surname:"Aqib",slug:"amjad-aqib",fullName:"Amjad Aqib",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/229220/images/system/229220.png",biography:"Dr. Amjad Islam Aqib obtained a DVM and MSc (Hons) from University of Agriculture Faisalabad (UAF), Pakistan, and a PhD from the University of Veterinary and Animal Sciences Lahore, Pakistan. Dr. Aqib joined the Department of Clinical Medicine and Surgery at UAF for one year as an assistant professor where he developed a research laboratory designated for pathogenic bacteria. Since 2018, he has been Assistant Professor/Officer in-charge, Department of Medicine, Manager Research Operations and Development-ORIC, and President One Health Club at Cholistan University of Veterinary and Animal Sciences, Bahawalpur, Pakistan. He has nearly 100 publications to his credit. His research interests include epidemiological patterns and molecular analysis of antimicrobial resistance and modulation and vaccine development against animal pathogens of public health concern.",institutionString:"Cholistan University of Veterinary and Animal Sciences",institution:{name:"University of Agriculture Faisalabad",country:{name:"Pakistan"}}},{id:"333753",title:"Dr.",name:"Rais",middleName:null,surname:"Ahmed",slug:"rais-ahmed",fullName:"Rais Ahmed",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/333753/images/20168_n.jpg",biography:null,institutionString:null,institution:{name:"University of Agriculture Faisalabad",country:{name:"Pakistan"}}},{id:"62900",title:"Prof.",name:"Fethi",middleName:null,surname:"Derbel",slug:"fethi-derbel",fullName:"Fethi Derbel",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/62900/images/system/62900.jpeg",biography:"Professor Fethi Derbel was born in 1960 in Tunisia. He received his medical degree from the Sousse Faculty of Medicine at Sousse, University of Sousse, Tunisia. He completed his surgical residency in General Surgery at the University Hospital Farhat Hached of Sousse and was a member of the Unit of Liver Transplantation in the University of Rennes, France. He then worked in the Department of Surgery at the Sahloul University Hospital in Sousse. Professor Derbel is presently working at the Clinique les Oliviers, Sousse, Tunisia. His hospital activities are mostly concerned with laparoscopic, colorectal, pancreatic, hepatobiliary, and gastric surgery. He is also very interested in hernia surgery and performs ventral hernia repairs and inguinal hernia repairs. He has been a member of the GREPA and Tunisian Hernia Society (THS). During his residency, he managed patients suffering from diabetic foot, and he was very interested in this pathology. For this reason, he decided to coordinate a book project dealing with the diabetic foot. Professor Derbel has published many articles in journals and collaborates intensively with IntechOpen Access Publisher as an editor.",institutionString:"Clinique les Oliviers",institution:null},{id:"300144",title:"Dr.",name:"Meriem",middleName:null,surname:"Braiki",slug:"meriem-braiki",fullName:"Meriem Braiki",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/300144/images/system/300144.jpg",biography:"Dr. Meriem Braiki is a specialist in pediatric surgeon from Tunisia. She was born in 1985. She received her medical degree from the University of Medicine at Sousse, Tunisia. She achieved her surgical residency training periods in Pediatric Surgery departments at University Hospitals in Monastir, Tunis and France.\r\nShe is currently working at the Pediatric surgery department, Sidi Bouzid Hospital, Tunisia. Her hospital activities are mostly concerned with laparoscopic, parietal, urological and digestive surgery. She has published several articles in diffrent journals.",institutionString:"Sidi Bouzid Regional Hospital",institution:null},{id:"229481",title:"Dr.",name:"Erika M.",middleName:"Martins",surname:"de Carvalho",slug:"erika-m.-de-carvalho",fullName:"Erika M. de Carvalho",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/229481/images/6397_n.jpg",biography:null,institutionString:null,institution:{name:"Oswaldo Cruz Foundation",country:{name:"Brazil"}}},{id:"186537",title:"Prof.",name:"Tonay",middleName:null,surname:"Inceboz",slug:"tonay-inceboz",fullName:"Tonay Inceboz",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/186537/images/system/186537.jfif",biography:"I was graduated from Ege University of Medical Faculty (Turkey) in 1988 and completed his Med. PhD degree in Medical Parasitology at the same university. I became an Associate Professor in 2008 and Professor in 2014. I am currently working as a Professor at the Department of Medical Parasitology at Dokuz Eylul University, Izmir, Turkey.\n\nI have given many lectures, presentations in different academic meetings. I have more than 60 articles in peer-reviewed journals, 18 book chapters, 1 book editorship.\n\nMy research interests are Echinococcus granulosus, Echinococcus multilocularis (diagnosis, life cycle, in vitro and in vivo cultivation), and Trichomonas vaginalis (diagnosis, PCR, and in vitro cultivation).",institutionString:"Dokuz Eylül University",institution:{name:"Dokuz Eylül University",country:{name:"Turkey"}}},{id:"71812",title:"Prof.",name:"Hanem Fathy",middleName:"Fathy",surname:"Khater",slug:"hanem-fathy-khater",fullName:"Hanem Fathy Khater",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/71812/images/1167_n.jpg",biography:"Prof. Khater is a Professor of Parasitology at Benha University, Egypt. She studied for her doctoral degree, at the Department of Entomology, College of Agriculture, Food and Natural Resources, University of Missouri, Columbia, USA. She has completed her Ph.D. degrees in Parasitology in Egypt, from where she got the award for “the best scientific Ph.D. dissertation”. She worked at the School of Biological Sciences, Bristol, England, the UK in controlling insects of medical and veterinary importance as a grant from Newton Mosharafa, the British Council. Her research is focused on searching of pesticides against mosquitoes, house flies, lice, green bottle fly, camel nasal botfly, soft and hard ticks, mites, and the diamondback moth as well as control of several parasites using safe and natural materials to avoid drug resistances and environmental contamination.",institutionString:null,institution:{name:"Banha University",country:{name:"Egypt"}}},{id:"99780",title:"Prof.",name:"Omolade",middleName:"Olayinka",surname:"Okwa",slug:"omolade-okwa",fullName:"Omolade Okwa",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/99780/images/system/99780.jpg",biography:"Omolade Olayinka Okwa is presently a Professor of Parasitology at Lagos State University, Nigeria. She has a PhD in Parasitology (1997), an MSc in Cellular Parasitology (1992), and a BSc (Hons) Zoology (1990) all from the University of Ibadan, Nigeria. She teaches parasitology at the undergraduate and postgraduate levels. She was a recipient of a Commonwealth fellowship supported by British Council tenable at the Centre for Entomology and Parasitology (CAEP), Keele University, United Kingdom between 2004 and 2005. She was awarded an Honorary Visiting Research Fellow at the same university from 2005 to 2007. \nShe has been an external examiner to the Department of Veterinary Microbiology and Parasitology, University of Ibadan, MSc programme between 2010 and 2012. She is a member of the Nigerian Society of Experimental Biology (NISEB), Parasitology and Public Health Society of Nigeria (PPSN), Science Association of Nigeria (SAN), Zoological Society of Nigeria (ZSN), and is Vice Chairperson of the Organisation of Women in Science (OWSG), LASU chapter. She served as Head of Department of Zoology and Environmental Biology, Lagos State University from 2007 to 2010 and 2014 to 2016. She is a reviewer for several local and international journals such as Unilag Journal of Science, Libyan Journal of Medicine, Journal of Medicine and Medical Sciences, and Annual Research and Review in Science. \nShe has authored 45 scientific research publications in local and international journals, 8 scientific reviews, 4 books, and 3 book chapters, which includes the books “Malaria Parasites” and “Malaria” which are IntechOpen access publications.",institutionString:"Lagos State University",institution:{name:"Lagos State University",country:{name:"Nigeria"}}},{id:"273100",title:"Dr.",name:"Vijay",middleName:null,surname:"Gayam",slug:"vijay-gayam",fullName:"Vijay Gayam",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/273100/images/system/273100.jpeg",biography:"Dr. Vijay Bhaskar Reddy Gayam is currently practicing as an internist at Interfaith Medical Center in Brooklyn, New York, USA. He is also a Clinical Assistant Professor at the SUNY Downstate University Hospital and Adjunct Professor of Medicine at the American University of Antigua. He is a holder of an M.B.B.S. degree bestowed to him by Osmania Medical College and received his M.D. at Interfaith Medical Center. His career goals thus far have heavily focused on direct patient care, medical education, and clinical research. He currently serves in two leadership capacities; Assistant Program Director of Medicine at Interfaith Medical Center and as a Councilor for the American\r\nFederation for Medical Research. As a true academician and researcher, he has more than 50 papers indexed in international peer-reviewed journals. He has also presented numerous papers in multiple national and international scientific conferences. His areas of research interest include general internal medicine, gastroenterology and hepatology. He serves as an editor, editorial board member and reviewer for multiple international journals. His research on Hepatitis C has been very successful and has led to multiple research awards, including the 'Equity in Prevention and Treatment Award” from the New York Department of Health Viral Hepatitis Symposium (2018) and the 'Presidential Poster Award” awarded to him by the American College of Gastroenterology (2018). He was also awarded 'Outstanding Clinician in General Medicine” by Venus International Foundation for his extensive research expertise and services, perform over and above the standard expected in the advancement of healthcare, patient safety and quality of care.",institutionString:"Interfaith Medical Center",institution:{name:"Interfaith Medical Center",country:{name:"United States of America"}}},{id:"93517",title:"Dr.",name:"Clement",middleName:"Adebajo",surname:"Meseko",slug:"clement-meseko",fullName:"Clement Meseko",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/93517/images/system/93517.jpg",biography:"Dr. Clement Meseko obtained DVM and PhD degree in Veterinary Medicine and Virology respectively. He has worked for over 20 years in both private and public sectors including the academia, contributing to knowledge and control of infectious disease. Through the application of epidemiological skill, classical and molecular virological skills, he investigates viruses of economic and public health importance for the mitigation of the negative impact on people, animal and the environment in the context of Onehealth. \r\nDr. Meseko’s field experience on animal and zoonotic diseases and pathogen dynamics at the human-animal interface over the years shaped his carrier in research and scientific inquiries. He has been part of the investigation of Highly Pathogenic Avian Influenza incursions in sub Saharan Africa and monitors swine Influenza (Pandemic influenza Virus) agro-ecology and potential for interspecies transmission. He has authored and reviewed a number of journal articles and book chapters.",institutionString:"National Veterinary Research Institute",institution:{name:"National Veterinary Research Institute",country:{name:"Nigeria"}}},{id:"158026",title:"Prof.",name:"Shailendra K.",middleName:null,surname:"Saxena",slug:"shailendra-k.-saxena",fullName:"Shailendra K. Saxena",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRET3QAO/Profile_Picture_2022-05-10T10:10:26.jpeg",biography:"Professor Dr. Shailendra K. Saxena is a vice dean and professor at King George's Medical University, Lucknow, India. His research interests involve understanding the molecular mechanisms of host defense during human viral infections and developing new predictive, preventive, and therapeutic strategies for them using Japanese encephalitis virus (JEV), HIV, and emerging viruses as a model via stem cell and cell culture technologies. His research work has been published in various high-impact factor journals (Science, PNAS, Nature Medicine) with a high number of citations. He has received many awards and honors in India and abroad including various Young Scientist Awards, BBSRC India Partnering Award, and Dr. JC Bose National Award of Department of Biotechnology, Min. of Science and Technology, Govt. of India. Dr. Saxena is a fellow of various international societies/academies including the Royal College of Pathologists, United Kingdom; Royal Society of Medicine, London; Royal Society of Biology, United Kingdom; Royal Society of Chemistry, London; and Academy of Translational Medicine Professionals, Austria. He was named a Global Leader in Science by The Scientist. He is also an international opinion leader/expert in vaccination for Japanese encephalitis by IPIC (UK).",institutionString:"King George's Medical University",institution:{name:"King George's Medical University",country:{name:"India"}}},{id:"94928",title:"Dr.",name:"Takuo",middleName:null,surname:"Mizukami",slug:"takuo-mizukami",fullName:"Takuo Mizukami",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/94928/images/6402_n.jpg",biography:null,institutionString:null,institution:{name:"National Institute of Infectious Diseases",country:{name:"Japan"}}},{id:"233433",title:"Dr.",name:"Yulia",middleName:null,surname:"Desheva",slug:"yulia-desheva",fullName:"Yulia Desheva",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/233433/images/system/233433.png",biography:"Dr. Yulia Desheva is a leading researcher at the Institute of Experimental Medicine, St. Petersburg, Russia. She is a professor in the Stomatology Faculty, St. Petersburg State University. She has expertise in the development and evaluation of a wide range of live mucosal vaccines against influenza and bacterial complications. Her research interests include immunity against influenza and COVID-19 and the development of immunization schemes for high-risk individuals.",institutionString:'Federal State Budgetary Scientific Institution "Institute of Experimental Medicine"',institution:null},{id:"238958",title:"Mr.",name:"Atamjit",middleName:null,surname:"Singh",slug:"atamjit-singh",fullName:"Atamjit Singh",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/238958/images/6575_n.jpg",biography:null,institutionString:null,institution:null},{id:"252058",title:"M.Sc.",name:"Juan",middleName:null,surname:"Sulca",slug:"juan-sulca",fullName:"Juan Sulca",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/252058/images/12834_n.jpg",biography:null,institutionString:null,institution:null},{id:"191392",title:"Dr.",name:"Marimuthu",middleName:null,surname:"Govindarajan",slug:"marimuthu-govindarajan",fullName:"Marimuthu Govindarajan",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/191392/images/5828_n.jpg",biography:"Dr. M. 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