Open access peer-reviewed chapter

Microencapsulation and Its Uses in Food Science and Technology: A Review

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Pedro Henrique Rodrigues do Amaral, Patrícia Lopes Andrade and Leilane Costa de Conto

Submitted: July 17th, 2018 Reviewed: October 12th, 2018 Published: September 27th, 2019

DOI: 10.5772/intechopen.81997

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Abstract

Microencapsulation is a group of technologies aiming to produce small particles called microcapsules that can be released at a specific speed under certain conditions. Microencapsulation technology is used in the pharmaceutical, agrochemical, and food industries; however, microcapsule production is most challenging for applications in the food industry owing to the high costs of the technique, which may make the final product too expensive. Common methods for microencapsulation include spray-drying and coacervation, and different wall materials and filling materials can be used for both techniques. In this review, we summarize current methodologies used for microencapsulation, with a focus on applications in the food industry.

Keywords

  • microencapsulation
  • food industry
  • nutrient enrichment
  • wall material
  • core

1. Introduction

Currently, food manufacturers and scientists worldwide are aiming to identify and characterize foods that can be used as sources of beneficial nutrients to promote the health and well-being of consumers. Based on this new paradigm, the development of new food products must combine novel technologies with the use of traditional methods to the control bio-accessibility of certain components in foods. As the interactions among health, nutrition, and genetics are clarified, this approach will become increasingly important.

One effective method for achieving these aims is microencapsulation [1], which was used as early as 1930. The first products containing microencapsulated materials were successfully fabricated in 1954. This advancement promoted further research on the use of microencapsulation in the pharmaceutical industry, wherein researchers found they could use these techniques to achieve controlled release of drugs in the body or in specific organs. Thus, pharmaceutical companies were crucial for developing improved techniques for microencapsulation [2]. In the 1960s, the first studies of microencapsulation in food technology were performed using essential oils; scientists attempted to prevent lipid oxidation, volatile compound losses, and aroma-controlled release. Subsequently, many more studies regarding microencapsulation of food products were published [3].

The goals of microencapsulation are to coat an active compound (core) by an encapsulating agent, also known as wall material, which will isolate the active material, thereby protecting the active material from adverse changes or to hide sensory properties that are not appreciated by consumers. The isolation provided by the encapsulating material will break under the application of a specific stimulus (e.g., pH or heat), releasing the active substance in the specific target location or under ideal conditions [2].

In this review, we summarize the latest applications of microencapsulation and microcapsule production methods in the food industry.

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2. Microencapsulation in the food industry

The techniques for producing microcapsules are significantly more challenging in the food industry than in other industries because the sensory qualities of foods cannot be compromised by the addition of encapsulated components. Furthermore, food matrices are more complex than those used in pharmaceutical and cosmetic industries. Moreover, in the food industry, microcapsules must be ingested orally, resist the adverse conditions of the gastrointestinal tract, and exhibit mucoadhesive properties [1]. Several different methods for microcapsule production have been developed, and microcapsules can be fabricated using various materials, which are chosen depending on the function of the microcapsules [4].

Microencapsulation is used to reduce adverse aromas, volatility, and reactivity of food products and to provide food products with greater stability when exposed to adverse conditions (e.g., light, O2, and pH) [5, 6]. Favaro-Trindade et al. [1] stated that microencapsulation is used in the food industry to reduce the reactivity of the active material in the external environment, reduce the speed of losses and evaporation of the core material into the medium, improve food handling, provide controlled release of the active product, mask unpleasant odor and taste, and allow the encapsulated material to be distributed in a food formulation homogeneously. However, microencapsulation is associated with dramatically increased costs of production, which may limit the economic viability of the method.

Notably, consumers are becoming increasingly aware of the importance of consuming meals that benefit health. Thus, products are being developed to provide health benefits to consumers; microencapsulation of various active compounds, such as vitamins, minerals, essential oils, and omega-3 polyunsaturated fat acids, among others, may be used to protect these compounds from nutrient loss and oxidation reactions and to hide sensory characteristics [2]. Therefore, while there are a wide range of applications of microencapsulation in the food industry, more studies are needed to determine the effectiveness of microencapsulation and the consumer acceptance of products manufactured using microencapsulation [7].

2.1. Microencapsulation processes

Microencapsulation is the science of trapping components (core or active) into a secondary material (encapsulant, wall material, carrier, or cover), producing small solid particles (1–500 μm in size) [8]. These particles are able to release their contents at a specified rate or under specific conditions [1].

The first step in microencapsulation consists of mixing the active material with the encapsulant material, making an emulsion. The mixture can be made with one or two agents. The mixture is then dried, producing microcapsules of different diameters and forms depending on the preparation method and materials used [7].

Physicochemical methods (simple or complex coacervation, separation of organic phase, and liposomal wrapping), physical methods (spray-drying, spray chilling, spray coating, fluidized bed, extrusion, centrifugation with multiple orifices, co-crystallization, and lyophilization), and chemical methods (interfacial polymerization and molecular inclusion) have been developed for microencapsulation [9].

Techniques and materials for microencapsulation are described in Table 1 [1].

The methods most used by the food industry and which deserve attention are described below.

2.1.1. Coacervation

The oldest microencapsulation technique and one of the most widely used techniques is coacervation, which involves macromolecular aggregates that form a colloidal system with two existing phases: one that is rich in colloids (coacervate) and one that is poor in colloids (supernatant). This method is performed by depositing the encapsulating agent around the active compound through physicochemical changes, such as temperature, polarity, pH, or ionic strength [2, 6].

Coacervation occurs when medium changes make the wall material form polymeric chain units, which then interact with others close chains, forming aggregates. After this step, the aggregates interact with each other through high-intensity attraction forces. Consequently, the aggregated polymer chains will be deposited around the droplets of the hydrophobic phase dispersed in the emulsion, forming a protective film [4].

The microcapsules obtained by coacervation can be classified as mononuclear or multinuclear according to their internal structure. When a drop of core material is encapsulated by coacervation, the particle formed is mononuclear; multinuclear particles are formed by aggregation of various mononuclear microcapsules. Multinuclear microcapsules have a matrix structure, and the core material can be released slowly unless the wall has been broken. However, mononuclear microcapsules have a vessel structure and release all their contents quickly. These particles are also irregular in structure because the wall material is not equally distributed over the surface of the core drop. The thinnest part of the wall layer will be more susceptible to disruption and release of the core.

Therefore, multinuclear microcapsules have greater controlled release and are produced more easily than mononuclear microcapsules [10].

The microcapsules produced by coacervation may have small diameters ranging from 1 to 500 μm for complex coacervation and from 20 to 500 μm for simple coacervation [1]. An example is presented in Figure 1. When using lipophilic materials with a hydrophilic coating, high encapsulation efficiency (85–90%) is generally observed [11, 12, 13, 14, 15].

Figure 1.

Microcapsules obtained by complex coacervation with gelatin/gum arabic (A) and soybean protein (B). Source: Author.

Complex coacervation has been used for microencapsulation of sensitive microorganisms and compounds, such as probiotics bacteria, omega-3 products, and bioactive compounds [16, 17, 18].

2.1.2. Spray-drying

The use of spray-drying for microencapsulation is another widely used technique due to its low-cost and easy application [19]. Spray-drying technique is used in the food industry for microencapsulating juice, pulp, and vegetal extracts [20, 21], probiotics [22], and fish oil [23].

During spray-drying, a homogeneous mixture of the active material and wall material in aqueous or organic solution is subjected to a hot airstream that promotes the evaporation of the solvent drying the microcapsules. Thus technique generates no solvent residues and does not require a washing process. However, the use of high temperatures may compromise the integrity of the core and wall materials. This microcapsule production process has a high efficiency rate, which can be affected by the concentration of the wall material, the system speed, and the feed temperature [24]. Moreover, spray-drying is more widely used than other methods owing to its relatively low cost and capacity for large-scale production [25].

However, according to Kolanowski et al. [11], spray-drying results in porous particles, and this characteristic may increase the susceptibility of the core material to oxidation. Additional disadvantages include the requirement for expensive equipment and the irregularity of the produced microcapsules [6, 24].

Table 2 shows some recent studies about spray-drying application on food microencapsulation.

Methods for encapsulationEncapsulated materials
Physical methods
Stationary extrusionLiquid/solid/gas
Submerged nozzleLiquid/solid/gas
Centrifugal extrusionLiquid/solid/gas
Vibrant nozzleLiquid/solid/gas
Spray-dryingLiquid/solid
Rotating discLiquid/solid
Pan coatingSolid
Air suspensionSolid
Spray chilling and spray coolingLiquid/solid
Fluidized bedSolid
Co-crystallizationLiquid/solid
LyophilizationLiquid
Chemical methods
Interfacial polymerizationLiquid/solid
Molecular inclusionLiquid
In situ polymerizationLiquid/solid
Physical-chemical methods
Simple coacervationLiquid/solid
Complex coacervationLiquid/solid
LiposomesLiquid/solid
Lipospheres (solid lipid nanoparticles and nanostructured lipid carriers)Liquid/solid
Evaporation of the solventLiquid/solid

Table 1.

Methods and kind of materials utilized for food products encapsulation.

PaperSource
Flavonoid microparticles by spray-drying: influence of enhancers of the dissolution rate on properties and stabilitySansone et al. [26]
Microencapsulation of linseed oil by spray-drying for functional food applicationGallardo et al. [27]
Optimization of microencapsulation of fish oil with gum arabic/casein/beta-cyclodextrin mixtures by spray-dryingLi et al. [28]
Retention of saffron bioactive components by spray-drying encapsulation using maltodextrin, gum arabic, and gelatin as wall materialsRajabi et al. [29]
Spray-drying microencapsulation of synergistic antioxidant mushroom extracts and their use as functional food ingredientsRibeiro et al. [30]
Spray-drying microencapsulation of cinnamon infusions (Cinnamomum zeylanicum) with maltodextrinSantiago-Adame et al. [31]
Influence of different combinations of wall materials on the microencapsulation of jussara pulp (Euterpe edulis) by spray-dryingSantana et al. [32]
Sulfur aroma compounds in gum arabic/maltodextrin microparticlesUekane et al. [33]

Table 2.

Spray-drying studies for microencapsulated food products.

2.1.3. Fluidized bed

In fluidized bed encapsulation, while the particles of the core material are suspended, the wall material is atomized into the chamber, depositing on the core. When the particles reach the top of the ascending column, they are released into a descending column of air which releases them back into the fluidized bed, where they are again coated, dried, and hardened, ensuring a uniform coating. Fluidized bed encapsulation is one of the few technologies that allow particles to be coated with any wall material (polysaccharides, proteins, emulsifiers, fats, etc.). This method has been used, for example, to isolate iron from ascorbic acid in multivitamin formulations or to encapsulate salt and acidulants avoiding, this way, the interaction of such ingredients with others [34].

Regardless of the method for microencapsulation, release of the core material depends on various factors, including pH, temperature, diffusion, medium solubility, mechanical rupture, and biodegradation. Additionally, the thickness of the encapsulating material may alter the stability and permeability of the microcapsules [1].

2.1.4. Molecular inclusion

One of the most promising possibilities of flavor stabilization is the formation of inclusion complex (molecular encapsulation) with β-cyclodextrin. Szente and Szejtli [35] investigating the stabilization of natural and synthetic coffee compounds with β-cyclodextrin, and thermal stability of this carbohydrate, observed the molecular encapsulation with natural and synthetic coffee compounds. They also noted that β-cyclodextrin is thermally destroyed at 260°C.

Inclusion compounds of β and γ-cyclodextrins with essential oils of lemon, orange, and camomile have been studied. Lemon and orange oils resulted in the union with β and γ-cyclodextrin. With camomile oil, the complex observed was only with γ-cyclodextrin [36].

2.2. Wall materials

The wall material should be able to form a film that is cohesive with the core material, be chemically compatible and nonreactive with the core material, and provide the desired coating properties, for example, strength, flexibility, impermeability, and stability [37]. In order to be able to be applied in food, the wall material must be food grade, biodegradable, and capable of forming a barrier between the active agent and the medium [19].

Importantly, some core materials are insoluble in aqueous solutions and may not easily form emulsions [38]. Specific proteins may function as emulsifiers, and polysaccharides contribute to the stability of emulsions; the interactions between proteins and carbohydrates can also help stabilize the emulsions.

Among the polysaccharides utilized as wall materials, gum arabic, maltodextrins, and modified starches are the most usual because of the high molecular weight and the high glass transition temperature [19]. However, other polysaccharides are also used, such as carrageenan, carboxymethylcellulose (CMC), and chitosan.

2.2.1. Polysaccharides

Gums are a group of polysaccharides and polysaccharide derivatives obtained from plants or secreted by bacteria and are commonly used for microcapsule production in the food industry.

Gum arabic has low viscosity in water, provides good retention of volatile compounds (>85%), and protects the core material from oxidation, which is crucial for microencapsulating essential oils and volatile substances [7]. Gum arabic has advantages for having this property emulsifier in a wide pH range, as well as other texturing, training film around the droplets and binding properties [38]. Conto et al. [17] studied the complex coacervation of soy proteins with gum arabic (GA); Renard et al. [39] worked with vitamin E microencapsulated on β-lactoglobulin/GA matrix.

Alternatively, alginate can be used for microencapsulation. This material forms strong, elastic gels with a distinct three-dimensional network. The gel network and homogeneity depends on the cation concentration; excess Ca2+ may result in multiple alginate chains having different physicochemical properties.

Alginate can also be used to produce microcapsules and cell immobilization through ionotropic gelation, which involves dropping the concentrated alginate solution into calcium chloride solution, externally gelling the polymer into a microcapsule. The size of the microcapsules formed using external gelation is governed by the size of droplets formed during the extrusion process [40] and ranges from tens of microns to millimeter size. Less commonly, microcapsules may be formed by internal gelation, in which the alginate in solution contains calcium carbonate [41].

Another common use of alginate microcapsules is to reduce the viability losses of probiotic bacteria, like Bifidobacteriumand Lactobacillus. Some works with probiotics alginate encapsulation are presented by Cook et al. [42] and are summarized in Table 3.

Encapsulation materialBacteriaReference apud Cook et al. [42]
AlginateLactobacillus acidophilusChandramouli et al. [40]
Alginate coated with palm oil and poly-l-lysine8 different Lactobacilliand BifidobacteriaDing et al. [43]
Alginate and xanthan gumLactobacillus acidophilusKim et al. [44]
Alginate coated with either chitosan, alginate, or poly-l-lysine-alginateLactobacillus acidophilus, Bifidobacterium bifidum, Lactobacillus caseiKrasaekoopt et al. [45]
AlginateLactobacillus caseiMandal et al. [46]
Alginate Alginate and pectinLactobacillus caseiSandoval-Castilla et al. [41]
Alginate coated with whey proteinLactobacillus plantarumGbassi et al. [47]
Alginate coated with chitosan, Sureteric, or Acryl-EZEBifidobacterium animalisLiserre et al. [48]
Alginate coated with chitosanBifidobacterium breveCook et al. [42]

Table 3.

Overview of literature available on the alginate encapsulation of probiotics cited by Cook et al. [42].

Carrageenans are widely used as thickening and stabilizing agents. Previous studies have reported microcapsules produced by carrageenan and oligochitosan polymer, but most reports have described the use of carrageenan for the encapsulation of microbial cells due to its capacity for gelation with the change in temperature from 40 to 45°C [49], suggesting the potential for use in probiotic foods.

Starch and modified starch can also be used as a wall material owing to its low viscosity, outstanding retention volatility (>93%), and ability to stabilize the emulsion with the core material [7]. Starches and their derivatives have been applied for the microencapsulation of vitamins, such as ascorbic acid [50, 51]. Maltodrextrin, which is inexpensive and has low hygroscopicity, can be used to prevent particle agglomeration [17] and has antioxidant effects [7].

Chitosan is also commonly used as a gelation agent in the food and pharmaceutical industries. Chitosan also allows concurrent cell permeabilization and immobilization; thus, chitosan-containing complexes of coacervated capsules have been widely explored [52].

Additionally, carboxymethylcellulose (CMC), an anionic water-soluble polymer, is used as an industrial agent owing to its capacity as a thickener, suspending agent, stabilizer, and binder. CMC forms resistant films that can protect against organic solvents, oils, and greases [53].

2.2.2. Protein films

Protein films are excellent oxygen and aroma barriers and can be used to produce microcapsules using coacervation techniques [54] or double emulsions with subsequent reticulation using glutaraldehyde or heat gelation [55]. Usually, proteins have been utilized with other biopolymers; some examples are presented in Table 4.

Wall materialCore materialSource
Gelatin/gum arabicSoybean oil, olive oil, and peanut oilRabišková, Valasková [68]
Gelatin/gum arabicEPALamprecht, Schäfer, Lehr [69]
Gelatin/gum arabicFish oilJouzel et al. [70]
Whey protein/gum arabicSunflower oil, lemon, and orange essential oilWeinbreck, Minor, DeKuif [58]
Hydroxpropyl methylcelluloseFish oilWu, Chai, Chen [71]
Gelatin/gum arabicOleoresin and soybean oilAlvim [72]
Gelatin/gum arabicBaking flavorYeo et al. [73]
Gelatin/pectin/gum arabicOilsPrata [74]
Gelatin/gum arabicPeppermint oilDong et al. [10]
GelatinStigmasterolOliveira [75]
SPI/pectinCasein hydrolyzateMedanha et al. [76]
b-Lg/pectinDHAZimet, Livney [77]
Gelatin/polyphosphateFish oil ethyl esterBarrow, Nolan, Holub [78]
Gelatin/gum arabicFlavorsLeclercq, Milo, Reineccius [79]
Gelatin/gum arabicSoybean oil and paprika resin oilCélis [80]
Gelatin/gum arabic1-Dodecanol (C12OH)Kong et al. [81]
HPMC/NaCMC/SDSSunflower oilKatona, Sovilj, Petrovic [82]
SPI/gum arabicOrange essential oilJun-Xia, Hai-Yan, Jian [13]

Table 4.

Overview of literature available on the proteins as encapsulant material.

Whey proteins (WPC and WPI) have also been investigated as wall materials for microencapsulation. For example, whey protein has been used for encapsulation of volatile and nonvolatile materials [56], typically through spray-drying, complex coacervation, heat gelation, and enzymatic gelation [57, 58]. Combinations such as whey protein isolate/gum arabic [59], β-lactoglobulin/pectin [60], β-lactoglobulin (b-Lg)/κ-carrageenan [61], whey protein/chitosan/gum arabic [62], and milk protein products/xanthan [63] are frequently used.

Despite these studies, proteins from plant sources have not been commonly used as carrier or wall materials in microencapsulation applications owing to limitations related to heat instability and organic solvent sensitivity. However, the use of reticulating agents to convert the proteins into a more stable form may improve their industrial applicability [64].

Additionally, soy proteins have the benefits of renewability, low-cost, and healthful effects [65]. Soy protein has high compatibility with gum arabic. SPI has been successfully used for microencapsulation of casein hydrolysate by spray-drying [66], orange essential oil by complex coacervation [13], and fish oil by enzymatic gelation [57]. Figure 2 presents microcapsules obtained with SPI by complex coacervation and enzymatic gelation.

Figure 2.

MEV of SPI microcapsules obtained by complex coacervation (A) and enzymatic gelation (B). Source: Author.

Gelatin can also be used as a foaming agent, emulsifier, and humectants in food, pharmaceutical, medical, and technical application sowing to its surface-active properties. Type A gelatin has a high isoelectric point and can form oil/water emulsions with positive charges at a wide range of pH values [67].

2.3. Core materials

Among core materials, essential oils are highly unstable and are sensitive to variations in light, air, temperature, and humidity. Therefore, new methods are needed to protect oils against these changes in order to increase their shelf life and their chemical stability under adverse conditions [1, 7].

Vitamins and minerals are generally added to foods to increase nutritional value, such as cereals, dairy products, infant foods, etc. However, these compounds can cause the food to taste unpleasant or may react with other food constituents, changing their sensory characteristics. Therefore, microencapsulation is widely used to protect vitamins and minerals against adverse conditions, such as temperature and humidity, to prevent undesirable reactions in food [1].

Microorganism microencapsulation has been applied to allow reuse of bacteria during the production of lactic acid and fermented milk products; increase production and cell concentrations in reactors; provide protection against oxygen gas, freezing temperatures, and the unfavorable pH of gastric juices and other acids; remove cell sand stop acidification; provide greater stability and maintain the viability of cultures during product storage; and increase their useful life [1].

Microencapsulation is widely used for enzyme immobilization, allowing reuse of enzymes and providing enzymes with superior stability; these features also reduced costs associated with the relevant processes. Moreover, microencapsulation for immobilization of enzymes is simple and permits the production of microcapsules having a variety of compositions [83].

Many studies have shown that consumption of omega-3 polyunsaturated fatty acids provides multiple health benefits, including reducing the risk of cardiovascular disease. Polyunsaturated fatty acids of the omega-3 group are mainly found in marine animals, such as plankton and fish in cold and deep waters, and fish oil has been the traditional source of these fatty acids. However, fish oil has an undesirable flavor. Thus, microencapsulation has been used for incorporation of fish oil as the core material, hiding the unpleasant sensory characteristics of the oil [17].

In studies with omega-3 microcapsules applied in food products, Chavez-Servín et al. [84] examined the addition of microencapsulated omega-3 fatty acids in infant formulas. Lysine and lactose degradation were observed; however, it was determined that the microcapsules did not affect the sensory acceptance of the final product. Moreover, Yep et al. [85] applied omega-3 microcapsules in bakery products and evaluated the effects of consumption of small daily doses of omega-3 fatty acids by intake of commercial bread compared to supplementation with capsules. They concluded that the effects depended on the amount of EPA and DHA in the blood of the individuals studied. Serna-Saldivar et al. [86] determined the shelf life of bread enriched with DHA and microencapsulated fish oil, showing that the development of off-flavors occurred more quickly in the breads containing liquid fish oil. Davidov-Pardo et al. [87] also observed changes in technological and sensory characteristics of breads containing omega-3 microcapsules.

The encapsulation of acids such as ascorbic, citric, fumaric, and lactic acids is usually carried out to avoid oxidation and allow them to be dissolved under specific conditions. Three specific applications stand out in this case: the dough improver, because the encapsulated ascorbic acid is often used to alter dough strength and improve slicing properties, color, and texture of baked products, being released during the proofing and baking stages, the aroma complement, and as an auxiliary agent in the meat processing, allowing the desired cured meat pigments to form [88].

Some natural colorants, such as urucum, β-carotene, and Curcuma, have solubility problems. It can be solved by encapsulation processes, which make them easier to handle during the process and improve the solubility and oxidation stability. Another advantage that can be associated with its use is in the shelf life extension, which can exceed 2 years, compared to the 6 months for non-encapsulated ones [88].

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3. Conclusion

Foods and other substances microencapsulated exhibit wide applicability, being an effective and extremely important tool in the preservation of various nutritional components, microorganisms, enzymes, dyes, etc., protecting food and other products from the most aggressive processing methods.

Several materials can be used as encapsulants, the most common being carbohydrates and some proteins, due to their higher affinity with various types of materials to be encapsulated. There are several methods of encapsulation by physical, chemical, and physicochemical, the most used being atomization, fluidized bed, and coacervation.

Despite the wide applicability, encapsulation has found little space in the food industry because of the cost. While the pharmaceutical and cosmetic sectors often support the use of high-cost techniques, the food industry works with lower profit margins, reducing production costs. In addition, industries often have strong resistance to the adoption of new technologies, due to the cost of implementation and the need for training.

Development of methodologies for incorporation of functional compounds in foods is needed to improve the health benefits and marketability of foods. Finally, microencapsulation of nutrients is a relatively new technology in the food industry, and further studies are needed to determine how to apply this technology most effectively.

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Acknowledgments

This work was partly funded by the Federal Institute of Triangulo Mineiro—Campus Uberlândia and a FAPEMIG research scholarship.

References

  1. 1. Favaro-Trindade CS, De Pinho SC, Rocha GA. Revisão: Microencapsulação de ingredientes alimentícios. Brazilian Journal of Food Technology. 2008;11:103-112
  2. 2. Suave J, Dall’agnol EC, Pezzin APT, Silva DAK, Meier MM, Soldi V. Microencapsulação: Inovação em diferentes áreas. Revista Saúde e Ambiente. 2006;7:12-20. ISSN 2175-1641
  3. 3. Gouin S. Microencapsulation: Industrial appraisal of existing technologies and trends. Food Science and Technology. 2004;15:330-347. DOI: 10.1016/j.tifs.2003.10.005
  4. 4. Leimann FV. Microencapsulação de óleo essencial de capim-limão utilizando o processo de coacervação simples [thesis]. Florianópolis: UFSC; 2008
  5. 5. Müller PS. Microencapsulação do óleo essencial de laranja [thesis]. Curitiba: Universidade Federal do Paraná; 2011
  6. 6. Foglio MA, Servat L, Spindola HM, Rodrigues RAF. Microencapsulação: Uma alternativa promissora para preservação de produtos naturais. Revista Fitos Eletrônica. 2013;5:52-57. ISSN: 2446-4927
  7. 7. Aburto LC, Tavares D d Q, Martucci ET. Microencapsulação de óleo essencial de laranja. Ciência e Tecnologia de Alimentos. 1998;18:45-48. DOI: 10.1590/S0101-20611998000100010
  8. 8. Sanguansri L, Augustin MA. Microencapsulation and delivery of omega-3 fatty acids. Functional food ingredients and nutraceuticals. In: Shi J, editor. Functional Food Ingredients and Nutraceuticals. Boca Raton: CRC Press; 2007. pp. 297-327
  9. 9. Jyothi NVN, Prasanna M, Prabha S, Seetha Ramaiah P, Srawan G, Sakarkar SN. Microencapsulation techniques, factors influencing encapsulation efficiency: A review. The Internet Journal of Nanotechnology. 2009;3:1-61. DOI: 10.5580/27bb
  10. 10. Dong ZJ, Toure A, Jia CS, Zhang XM, Xu SY. Effect of processing parameters on the formation of spherical multinuclear microcapsules encapsulating peppermint oil by coacervation. Journal of Microencapsulation. 2007;24:634-646. DOI: 10.1080/02652040701500632
  11. 11. Kolanowski W, Jaworska D, Weiâbrodt J, Kunz B. Sensory assessment of microencapsulated fish oil powder. Journal of the American Oil Chemists’ Society. 2007;84:37-45. DOI: 10.1007/s11746-006-1000-x
  12. 12. Martins IM, Barreiro MF, Coelho M, Rodrigues AE. Microencapsulation of essential oils with biodegradable polymeric carriers for cosmetic applications. Chemical Engineering Journal. 2014;245:191-200. DOI: 10.1016/j.cej.2014.02.024
  13. 13. Jun-Xia X, Hai-Yan Y, Jian Y. Microencapsulation of sweet orange oil by complex coacervation with soybean protein isolate/gum arabic. Food Chemistry. 2011;125:1267-1272. DOI: 10.1016/j.foodchem.2010.10.063
  14. 14. Comunian TA, Thomazini M, Alves AJG, Matos Junior FE, Carvalho Balieiro JC, Favaro-Trindade CS. Microencapsulation of ascorbic acid by complex coacervation: Protection and controlled release. Food Research International. 2013;52:373-379. DOI: 10.1016/j.foodres.2013.03.028
  15. 15. Lima JR, Locatelli GO, Finkler L, Luna-Finkler CL. Incorporação deLactobacillus caseimicroencapsulado em queijo tipo coalho. Ciência & Saúde. 2014;7:27-34. DOI: 10.15448/1983-652X.2014.1.15639
  16. 16. Simeoni CP, Etchepare MA, Menezes CR, Fries LM, Menezes MFC, Stefanello FS. Microencapsulation of probiotics: Technological innovation in the food industry. Revista do Centro de Ciências Naturais e Exatas. 2014;18:66-75. DOI: 10.5902/2236117013020
  17. 17. Conto LC, Grosso CRG, Gonçalves LAG. Chemometry as applied to the production of omega-3 microcapsules by complex coacervation with soy protein isolate and gum arabic. LWT—Food Science and Technology. 2013;53:218-224
  18. 18. Silva TM, Rodrigues LZ, Nunes GL, Codevilla CF, Silva CB, Menezes CR. Encapsulation of bioactive compounds by complex coacervation. Ciência e Natura. 2015;37:56-64. DOI: 10.5902/2179-460X19715
  19. 19. Pereira KC, Ferreira DCM, Alvarenga GF, Pereira MSS, Barcelos MCS, Costa JMG. Microencapsulation and release controlled by the diffusion of food ingredients produced by spray drying: A review. Brazilian Journal of Food Technology. 2018;211:e2017083. DOI: 10.1590/1981-6723.08317
  20. 20. Da Silva MV, Junior BD, Visentainer JV. Production and characterization of maltodextrins and its application in microencapsulation of food compounds by spray drying. Revista Ciências Exatas e Naturais. 2014;16:11-126. DOI: 10.5935/RECEN.2014.01.07
  21. 21. Santana AA, Oliveira RA, Kurozawa LE, Park KJ. Microencapsulação de polpa de pequi por spray drying: Uso de dextrina como agente encapsulante. Engenharia Agrícola. 2014;34:980-991. DOI: 10.1590/S0100-69162014000500017
  22. 22. Menezes CR, Barin JS, Chicoski AJ, Zepka LQ, Jacob-Lopes E, Friesi LLM, et al. Microencapsulation of probiotics: Progress and prospects. Ciência Rural. 2013;43:1309-1316. ISSN 0103-8478
  23. 23. Kuhn F, Dal Magro J, Raja S, Ferrando M. Encapsulation of fish oil for food use by membrane emulsification followed by atomization. Revista Brasileira de Pesquisa em Alimentos. 2014;5:1-8. DOI: 10.14685/rebrapa.v5i1.133
  24. 24. Silva C, Ribeiro A, Ferreia D, Veiga F. Oral delivery system for peptides and proteins: II. Application of microencapsulation methods. Brazilian Journal of Pharmaceutical Sciences. 2003;39:1-20. DOI: 10.1590/S1516-93322003000100002
  25. 25. Lee SJ, Ying DY. Encapsulation of fish oils. In: Garti N, editor. Delivery and Controlled Release of Bioactives Food Nutraceuticals. Boca Raton: CRC; 2008
  26. 26. Sansone F, Picerno P, Mencherini T, Villecco F, D’Ursi AM, Aquino RP, et al. Flavonoid microparticles by spray-drying: Influences of enhancers of the dissolution rate on properties and stability. Journal of Food Engineering. 2011;103:188-196. DOI: 10.1016/j.jfoodeng.2010.10.015
  27. 27. Gallardo G, Guida L, Martinez V, López MC, Bernhardt D, Blasco R, et al. Microencapsulation of linseed oil by spray drying for functional food application. Food Research International. 2013;52:473-482. DOI: 10.1016/j.foodres.2013.01.020
  28. 28. Li J, Xiong S, Wang F, Regenstein JM, Liu R. Optimization of microencapsulation of fish oil with gum arabic, casein, beta-cyclodextrin mixtures by spray drying. Journal of Food Science. 2015;80:1445-1452. DOI: 10.1111/1750-3841.12928
  29. 29. Rajabi H, Ghorbani M, Jafari SM, Sadeghi Mahoonak A, Rajabzadeh G. Retention of saffron bioactive components by spray drying encapsulation using maltodextrin, gum arabic and gelatin as wall materials. Food Hydrocolloids. 2015;51:327-337. DOI: 10.1016/j.foodhyd.2015.05.033
  30. 30. Ribeiro A, Ruphuy G, Lopes JC, Dias MM, Barros L, Barreiro F, et al. Spray-drying microencapsulation of synergistic antioxidant mushroom extracts and their use as functional food ingredients. Food Chemistry. 2015;188:612-618. DOI: 10.1016/j. foodchem.2015.05.061
  31. 31. Santiago-Adame R, Medina-Torres L, Gallegos-Infante JA, Calderas F, Gonzáles-Laredo RF, Rocha-Guzmán NE, et al. Spray drying microencapsulation of cinnamon infusions (Cinnamomum zeylanicum) with maltodextrin. Food Science and Technology. 2015;64:571-577. DOI: 10.1016/j.lwt.2015.06.020
  32. 32. Santana AA, Cano-Higuita DM, Oliveira RA, Telis VRN. Influence of different combinations of wall materials on the microencapsulation of jussara pulps (Euterpe edulis) by spray drying. Food Chemistry. 2016;212:1-9. DOI: 10.1016/j.foodchem.2016.05.148
  33. 33. Uekane TM, Costa ACP, Pierucci APTR, Rocha-Leão MHM, Rezende CM. Sulfur aroma compounds in gum arabic/maltodextrin microparticles. Lebensmittel-Wissenschaft und Technologie. 2016;70:342-348. DOI: 10.1016/j. lwt.2016.03.003
  34. 34. Azeredo HMC. Encapsulation: Applications to food technology. Alimentos e Nutrição. 2005;16:89-97. ISSN 0103-4235
  35. 35. Szente L, Szejtli J. Molecular encapsulation of natural and synthetic coffee flavor with β-cyclodextrin. Journal of Food Science. 1986;51:1024-1027
  36. 36. Thoss M, Schawabe L, Fromming KH. Cyclodextrin inclusion compounds of lemon oil, orange oil, hop oil and chamomile oil. PZ Wissenschaft. 1993;138:144-148
  37. 37. Bansode SS, Banarjee SK, Gaikwad DD, Jadhav SL, Thorat RM. Microencapsulation: A review. International Journal of Pharmaceutical Sciences Review and Research. 2010;1:38-43. ISSN: 0975-6299
  38. 38. Gharsallaoui A, Roudaut G, Chambin O, Voilley A, Saurel R. Applications of spray-drying in microencapsulation of food ingredients: An overview. Food Research International. 2007;40:1107-1121. DOI: 10.1016/j.foodres.2007.07.004
  39. 39. Renard D, Robert P, Lavenant L, Melcin D, Popineau Y, Gueguen J, et al. Biopolymeric colloidal carriers for encapsulation or controlled release applications. International Journal of Pharmaceutics. 2002;242:163-166. DOI: 10.1016/S0378-5173(02)00143-6
  40. 40. Chandramouli V, Kailasapathy K, Peiris P, Jones M. An improved method of microencapsulation and its evaluation to protectLactobacillusspp. in simulated astric conditions. Journal of Microbiological Methods. 2004;56:27-35. DOI: 10.1016/j.mimet.2003.09.002
  41. 41. Sandoval-Castilla O, Lobato-Calleros C, García-Galindo HS, Alvarez-Ramírez J, Vernon-Carter EJ. Textural properties of alginate-pectin beads and survivability of entrappedLb. caseiin simulated gastrointestinal conditions and in yoghurt. Food Research International. 2010;43:111-117. DOI: 10.1016/j.foodres.2009.09.010
  42. 42. Cook MT, Tzortzis G, Charalampopoulos D, Khutoryanskiy VV. Microencapsulation of probiotics for gastrointestinal delivery. Journal of Controlled Release. 2012;162:56-67. DOI: 10.1016/j.jconrel.2012.06.003
  43. 43. Ding WK, Shah NP. An improved method of microencapsulation of probiotic bacteria for their stability in acidic and bile conditions during storage. Journal of Food Science. 2009;74:M53-M61. DOI: 10.1111/j.1750-3841.2008.01030.x
  44. 44. Kim S-J, Cho SY, Kim SH, Song O-J, Shin IIS, Cha DS, et al. Effect of microencapsulation on viability and other characteristics in Lactobacillus acidophilus ATCC 43121. LWT—Food Science and Technology. 2008;41:493-500. DOI: 10.1016/j.lwt.2007.03.025
  45. 45. Krasaekoopt W, Bhandari B, Deeth H. The influence of coating materials on some properties of alginate beads and survivability of microencapsulated probiotic bacteria. International Dairy Journal. 2004;14:737-743. DOI: 10.1016/j.idairyj.2004.01.004
  46. 46. Mandal S, Puniya AK, Singh K. Effect of alginate concentrations on survival of microencapsulatedLactobacillus caseiNCDC-298. International Dairy Journal. 2006;16:1190-1195. DOI: 10.1016/j.idairyj.2005.10.005
  47. 47. Gbassi GK, Vandamme T, Ennahar S, Marchioni E. Microencapsulation ofLactobacillus plantarumspp in an alginate matrix coated with whey proteins. International Journal of Food Microbiology. 2009;129:103-105. DOI: 10.1016/j.ijfoodmicro.2008.11.012
  48. 48. Liserre AM, Re MI, Franco B. Microencapsulation ofBifidobacteriumanimalis subsp lactis in modified alginate-chitosan beads and evaluation of survival in simulated gastrointestinal conditions. Food Biotechnology. 2007;21:1-16. DOI: 10.1080/08905430701191064
  49. 49. Bartkowiak A, Hunkeler D. Carrageenan-oligochitosan microcapsules: Optimization of the formation process. Colloids and Surfaces B: Biointerfaces. 2001;21:285-298. DOI: 10.1016/S0927-7765(00)00211-3
  50. 50. Trindade MA, Grosso CRF. The stability of ascorbic acid microencapsulated in granules of rice starch and in gum arabic. Journal of Microencapsulation. 2000;17:169-176. DOI: 10.1080/026520400288409
  51. 51. Uddin MS, Hawlader MNA, Zhu HJ. Microencapsulation of ascorbic acid: Effect of process variables on product characteristics. Journal of Microencapsulation. 2001;18:199-209. DOI: 10.1080/02652040010000352
  52. 52. Pegg RB, Shahidi F. Encapsulation, stabilization, and controlled release of food ingredients and bioactives. In: Rahman MS, editor. Handbook of Food Preservation. Boca Raton: CRC Press; 2007. pp. 509-568
  53. 53. Nisperos-Carriedo MO. Edible coatings and films based on polysaccharides. In: Krochta JM, Baldwin EA, Nisperos-Carriedo MO, editors. Edible Coatings and Films to Improve Food Quality. Lancaster: Technomic Publishing Co. Inc.; 1994. p. 305
  54. 54. Conto LC, Fernandes GD, Grosso CRF, Eberlin MN, Gonçalves LAG. Evaluation of the fatty matter contained in microcapsules obtained by double emulsification and subsequent enzymatic gelation method. Food Research International. 2013;54:432-438. DOI: 10.1016/j.foodres.2013.07.013
  55. 55. Lee SJ, Rosenberg M. Whey protein-based microcapsules prepared by double emulsification and heat gelation. LWT—Food Science and Technology. 2000;33:80-88. DOI: 10.1006/fstl.1999.0619
  56. 56. Rosenberg M. Milk Derived Whey Protein-based Microencapsulating Agents and A Method of Use. US Patent Number 5601760; 1997
  57. 57. Cho YH, Shim HK, Park J. Encapsulation of fish oil by an enzymatic gelation process using transglutaminase cross-linked protein. Journal of Food Science. 2003;68:2717-2723. DOI: 10.1111/j.1365-2621.2003.tb05794
  58. 58. Weinbreck F, Minor M, De Kruif CG. Microencapsulation of oils using whey protein/gum arabic coacervates. Journal of Microencapsulation. 2004;21:667-679. DOI: 10.1080/02652040400008499
  59. 59. Klein M, Aserin A, Svitov I, Garti N. Enhanced stabilization of cloudy emulsions with gum arabic and whey protein isolate. Colloids and Surfaces B: Biointerfaces. 2010;77:75-81. DOI: 10.1016/j.colsurfb.2010.01.008
  60. 60. Guzey D, Kim HJ, McClements DJ. Factors influencing the production of o/w emulsions stabilized by β-lactoglobulin-pectin membranes. Food Hydrocolloids. 2004;18:967-975. DOI: 10.1016/j.foodhyd.2004.04.001
  61. 61. Gu YS, Regnier L, McClements DJ. Influence of environmental stress on stability of oil-in-water emulsions containing droplets stabilized by β-lactoglobulin-κ-carrageenan membranes. Journal of Colloid and Interface Science. 2005;286:551-558. DOI: 10.1016/j.jcis.2005.01.051
  62. 62. Moschakis T, Murray BS, Biliaderis CG. Modifications in stability and structure of whey protein-coated o/w emulsions by interacting chitosan and gum arabic mixed dispersions. Food Hydrocolloids. 2010;24:8-17. DOI: 10.1016/j.foodhyd.2009.07.001
  63. 63. Hemar Y, Tamehana M, Munto PA, Singh H. Viscosity, microstructure and phase behaviour of aqueous mixtures of commercial milk protein products and xanthan gum. Food Hydrocolloids. 2001;15:565-574. DOI: 10.1016/S0268-005X(01)00077-7
  64. 64. Nesterenko A, Alric I, Silvestre F, Durrieu V. Vegetable proteins in microencapsulation: A review of recent interventions and their effectiveness. Industrial Crops and Products. 2013;42:469-479. DOI: 10.1016/j.indcrop.2012.06.035
  65. 65. Tang C-H, Li X-R. Microencapsulation properties of soy protein isolate and storage stability of the correspondingly spray-dried emulsions. Food Research International. 2012;52:419-428. DOI: 10.1016/j.foodres.2012.09.010
  66. 66. Molina-Ortiz SE, Mauri A, Monterrey-Quintero ES, Trindade MA, Santana AS, Favaro-Trindade CS. Production and properties of casein hydrolysate microencapsulated by spray drying with soybean protein isolate. LWT—Food Science and Technology. 2009;42:919-923. DOI: 10.1016/j.lwt.2008.12.004
  67. 67. Dickinson E, Lopez G. Comparison of the emulsifying properties of fish gelatin and commercial fish proteins. Journal of Food Science. 2001;66:118-123. DOI: 10.1111/j.1365-2621.2001.tb15592.x
  68. 68. Rabišková M, Valášková J. The influence of HLB on the encapsulation of oils by complex coacervation. Journal of Microencapsulation. 1998;15:747-751. DOI: 10.3109/02652049809008257
  69. 69. Lamprecht A, Schafer UF, Lehr CM. Influences of process parameters on preparation of microparticle used as a carrier system for O-3 unsaturated fatty acid ethyl esters used in supplementary nutrition. Journal of Microencapsulation. 2001;18:347-357. DOI: 10.1080/02652040010000433
  70. 70. Jouzel B, Pennarun A-L, Prost C, Renard D, Poncelet D, Demaimay M. Encapsulation of a lipid precursor, the eicosapentaenoic acid, to study the development of theCrassostrea gigasoyster flavours. Journal of Microencapsulation. 2003;20(1):35-46. DOI: 10.3109/02652040309178047
  71. 71. Wu KG, Chai XH, Chen Y. Microencapsulation of fish oil by simple coacervation of hydroxypropyl methylcellulose. Chinese Journal of Chemistry. 2005;23:1269-1572. DOI: 10.1002/cjoc.200591569
  72. 72. Alvim ID. Produção e caracterização de micropartículas obtidas por spray drying e coacervação complexa e seu uso para alimentação de larvas de peixes [thesis]. Faculdade de Engenharia de Alimentos: Universidade Estadual de Campinas; 2005
  73. 73. Yeo Y, Bellas E, Firestona W, Languer R, Kohane DS. Complex coacervates for thermally sensitive controlled release of flavour compounds. Journal of Agricultural and Food Chemistry. 2005;53:7518-7525. DOI: 10.1021/jf0507947
  74. 74. Prata AS. Estudos dos parâmetros físico-químicos envolvidos na formação de microcápsulas produzidas por coacervação complexa [thesis]. Faculdade de Engenharia de Alimentos: Universidade Estadual de Campinas; 2006
  75. 75. Oliveira AB. Microencapsulamento de Estigmasterol proveniente deMusa paradisiacaL., Musaceae [thesis]. Ciências Farmacêuticas, Setor de Ciências da Saúde: Universidade Federal do Paraná; 2007
  76. 76. Medanha DV, Ortiz SEM, Favaro-Trindade CS, Mauri A, Monterrey-Quintero ES, Thomazini M. Microencapsulation of casein hydrolysate by complex coacervation with SPI/pectin. Food Research International. 2009;42:1099-1104. DOI: 10.1016/j.foodres.2009.05.007
  77. 77. Zimet P, Livney Y. Beta-lactoglobulin and its nanocomplexes with pectin as vehicles for w-3 polyunsaturated fatty acids. Food Hydrocolloids. 2009;23:1120-1126. DOI: 10.1016/j.foodhyd.2008.10.008
  78. 78. Barrow CJ, Nolan C, Holub BJ. Bioequivalence of encapsulated and microencapsulated fish-oil supplementation. Journal of Functional Foods. 2009;1:38-43. DOI: 10.1016/j.jff.2008.09.006
  79. 79. Leclercq S, Milo C, Reineccius GA. Effects of cross-linking, capsule wall thickness, and compound hydrophobicity on aroma release from complex coacervate microcapsules. Journal of Agricultural and Food Chemistry. 2009;57:1426-1432. DOI: 10.1021/jf802472q
  80. 80. Célis FT. Efeito da reticulação induzida pela transglutaminase e o glutaraldeido sobre as propriedades das micropartículas obtidas por coacervação complexa [thesis]. Faculdade de Engenharia de Alimentos: Universidade Estadual de Campinas; 2009. 148 p
  81. 81. Kong XZ, Gu X, Zhu X, Zhang Z. Spreadable dispersion of insect sex pheromone capsules, preparation via complex coacervation and release control of the encapsulated pheromone component molecule. Biomedical Microdevices. 2009;11:275-285. DOI: 10.1007/s10544-008-9234-z
  82. 82. Katona JM, Sovilj VJ, Petrovic LB. Microencapsulation of oil by polymer mixture-ionic surfactant interaction induced coacervation. Carbohydrate Polymers. 2010;79:563-570. DOI: 10.1016/j.carbpol.2009.09.007
  83. 83. Oliveira IRWZ, Fatibello-Filho O, Fernandes SC, Vieira IC. Imobilização da lactase em micropartículas de quitosana obtidas por spray drying e usadas na construção de biossensores. Quimica Nova. 2009;32:1195-1201. DOI: 10.1590/S0100-40422009000500021
  84. 84. Chávez-Servín JL, Castellote AI, López-Sabater M. Evolution of available lysine and lactose contents in supplemented microencapsulated fish oil infant formula powder during storage. International Journal of Food Science and Technology. 2008;43:1121-1128. DOI: 10.1111/j.1365-2621.2007.01588.x
  85. 85. Yep YL, Li D, Mann NJ, Bode O, Sinclair AJ. Bread enriched with microencapsulated tuna oil increases plasma docosahexaenoic acid and total omega-3 fatty acids in humans. Asia Pacific Journal of Clinical Nutrition. 2002;11:285-291. DOI: 10.1046/j.1440-6047.2002.00309.x
  86. 86. Serna-Saldivar SO, Zorrilla R, La Parra C, Stagnitti G, Abril R. Effect of DHA containing oils and powders on baking performance and quality of white pan bread. Plant Foods for Human Nutrition. 2006;61:121-129. DOI: 10.1007/s11130-006-0009-5
  87. 87. Davidov-Pardo G, Roccia P, Salgado D, León AE, Pedroza-Islas R. Utilization of different wall materials to microencapsulate fish oil evaluation of its behavior in bread products. American Journal of Technology. 2008;3:384-393. DOI: 10.3923/ajft.2008.384.393
  88. 88. Rebello FFP. Microencapsulação de ingredientes alimentícios. Revista Agrogeoambiental. 2009;1:134-144. DOI: 10.18406/2316-1817v1n32009223

Written By

Pedro Henrique Rodrigues do Amaral, Patrícia Lopes Andrade and Leilane Costa de Conto

Submitted: July 17th, 2018 Reviewed: October 12th, 2018 Published: September 27th, 2019