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Observation on the productivity parameters in relation to micro algal biodiversity helps to know the population in particular season and spatial. The study investigates in detail the seasonal and spatial variation of microalgae with special emphasis on their interrelationship of chlorophyll concentration. In order to obtain the information on distribution and abundance of Visakhapatnam Coast microalgae for isolation, fortnightly intervals samplings was carried out. Investigation has been made on the microalgae with special reference to the phylum Ochrophyta, Dinophyta, Chlorophyta, Euglenozoa, Haptophyta and Cyanophyta. Abundance of species under different season of pattern was Pre-monsoon>Post monsoon>Monsoon. The data evaluated from this study was used to prepare the checklist for marine microalgal diversity of Visakhapatnam offshore region.
Council of Scientific and Industrial Research, National Institute for Interdisciplinary Science and Technology, Environmental Technology Division, Govt. of India, India
Athia Shameem
Marine Living Resources, Andhra University, India
Katru Umadevi
Marine Living Resources, Andhra University, India
Boddu Sivaprasad
Marine Living Resources, Andhra University, India
Ajith Haridas
Council of Scientific and Industrial Research, National Institute for Interdisciplinary Science and Technology, Environmental Technology Division, Govt. of India, India
*Address all correspondence to: bioselvas@gmail.com
1. Introduction
Andhra Pradesh is one of the six States/U.Ts of India adjoining the Bay of Bengal with a coastline of 974 km and the continental shelf area of 33, 227 sq. km. East coast India, surface currents skirting the coast move in a northerly direction during part of the year, and the opposite direction during the rest of the year [1]. Influx of untreated wastewaters into the aquatic bodies that are challenging the stability of nations [2]. Since in the middle of 19th century East coast of India, Visakhapatnam coastal waters pollution caused by the effluents from nearby industries, like steel plant, petroleum refinery, fertilizer plant and a lead and zinc smelter are discharged into a North-Western arm of the inner harbor through surface drain known as Meghadri gedda and city’s domestic sewage drains directly into the Northern arm of the inner harbor [3]. Microalgal abundance and distribution critically depending on various physical, chemical and biological factors [4] and their ability to assimilate sufficient carbon, nitrogen and phosphorous, as well as minor nutrients, to ensure replication. Alterations in species richness are mainly due to the variability of abiotic factors, such as short-term climatic variations [5].
Multi-population microalgae and some native isolates i.e., Tetraselmis sp., Chlorella sp. were cultivated in open air pond in East cost of India proved the potential for biofuel production. Exploring microalgae diversity to find out the suitable season and spatial for microalgae isolation in Visakhapatnam coast since, the microalgae having wide application in biofuel and pharmaceutical. The research work from Andhra University, India revealed that Visakhapatnam coast is one of the potential sources for microalgae. Two years field work data from this study shows the relationship between the spatial and seasonal variations in Bay of Bengal, East Coast of India, and Visakhapatnam. Qualitative and quantitative analysis showed the feasibility to isolate the potential candidate strains for biofuels from the coastal water of Visakhapatnam.
2.1. Description of the study area and physiography of sampling sites
Bay of Bengal, a semi-enclosed tropical basin, is a part of the northern Indian Ocean and experiences seasonal changes in circulation and climate due to the monsoons. Visakhapatnam is a coastal city located on the eastern seaboard of India between Chennai and Calcutta (latitude 17°38’N and 17°45’N and longitude 83°16′E and 83°21′E) surrounded on three sides by the overlapping mountain ranges, and the South-eastern city is safeguarded by the Bay of Bengal. The South-west monsoon starts late in June and lasts till early October. North-east monsoon closely follows the South-west monsoon and extends till December. Fourteen sampling stations were selected along the Visakhapatnam offshore line and surface water samples were collected at the 30–40 M depth line of the sampling point. Sampling sites and global positioning systems of sampling locations was represented in Figure 1. The sampling stations between Bheemunipatnam (station 1) and Coastal Battery (station 7) cover the area like waste water of shrimp culture ponds, seafood processing centers, shrimp hatcheries, fish cages cultures and sewage outfalls enter into the coastline. The sampling stations between Harbor (station 8) and Pudimadaka (station 14) cover the areas where the effluents from the major power plants such as Nuclear Power Corporation of India limited and East Coast Energy limited enter on the coastal line.
Figure 1.
Map showing the sampling stations (1–14).
2.2. Sample collection and estimation of chlorophyll
A mechanized boat was used to get into the sea for sample collection. Physico-chemical examination of sea surface waters in relation to microalgal abundance from Bheemunipatnam (approximately 23 km northward from the Visakhapatnam port) to Pudimadaka (approximately 40 km southward from the Visakhapatnam port), on the East Coast of India, was carried out at fortnightly intervals for a period of 2 years (February 2011 to January 2013) at 14 selected stations, along the Visakhapatnam offshore region. Samples were collected using clean plastic buckets from the surface sea water and transferred into 1 L amber color plastic bottles and stored in refrigerator until further analysis. The water sample free of zooplankton is filtered through Millipore (0.45 μm, 47 mm) filter paper and the pigments chlorophyll ‘a’, ‘b’ and ‘c’ are extracted from the phytoplankton by using 90% acetone.
2.3. Collection of microalgae and identification
One hundred liters of sea surface water at each station was collected and filtered through cone-shaped phytoplankton net of 20-μm mesh size, made by bolting silk and concentrated to 500 ml was transferred into pre-cleaned polyethylene bottles. The filtrate was preserved in 3% neutralized Lugol’s iodine solution. All the water samples were filtered with 60-μm size zooplankton net in order to remove the zooplankton and debris. The filtrate was collected into five liters capacity Polyethylene Terephthalate (PET) jar and kept undisturbed for 2 days to achieve complete sedimentation. After sedimentation of phytoplankton, the supernatant solution was siphoned out to concentrate the volume to accurately about 100–200 ml. Concentrated samples were examined under bright field, dark ground illumination and phase contrast at ×200, ×400 and ×1000 magnifications with the help of Axio scope A1 and Primo Vert (Carl Zeiss, Germany) microscopes. Identification of microalgae was done using an inverted research microscope based on standard keys [6, 7, 8, 9, 10, 11, 12, 13, 14, 15] and current taxonomical details were been updated according to World Register of Marine Species and Algaebase (2015).
In the year 2011–2012, the highest and lowest mean chlorophyll ‘a’ recorded in station 13 (PRM) and station 9 (MON) were 4.81 ± 2.86 and 0.68 ± 0.36 μg l−1, respectively. During the post monsoon period, the maximum and minimum chlorophyll ‘a’ recorded was 1.82 ± 1.53 and 0.85 ± 0.55 μg l−1 respectively at station 9 and station 7 (Figure 2a). In the year 2012–2013, the highest and lowest mean chlorophyll ‘a’ recorded in station 9 (MON) and station 3 (PRM) were 3.76 ± 1.92 and 1.54 ± 1.04 μg l−1, respectively. During the post monsoon period, the maximum and minimum chlorophyll ‘a’ recorded was 2.66 ± 1.79 and 1.92 ± 1.34 μg l−1 respectively at station 7 and station 2 (Figure 2b). In the both sampling years, two way ANOVA showed significant differences between the season (p < 0.001) but not between the stations.
Figure 2.
Seasonal and spatial variations in chlorophyll ‘a’ (a) for the sampling year 2011–2012 and (b) for the sampling year 2012–2013.
3.2. Chlorophyll ‘b’
In the year 2011–2012, the highest and lowest mean chlorophyll ‘b’ recorded in station 5 (PRM) and station 7 (POM) were 3.15 ± 2.28 and 0.70 ± 0.51 μg l−1, respectively. During the monsoon period, the maximum and minimum Chlorophyll ‘b’ recorded was 1.99 ± 1.58 and 0.99 ± 0.48 μg l−1 respectively at station 4 and station 8 (Figure 3a). In the year 2012–2013, the highest and lowest mean chlorophyll ‘b’ recorded in station 6 (MON) and station 11 (PRM) were 4.14 ± 2.73 and 1.50 ± 1.35 μg l−1, respectively. During the post monsoon period, the maximum and minimum chlorophyll ‘b’ recorded was 2.51 ± 1.79 and 1.69 ± 0.73 μg l−1 respectively at station 7 and station 13 (Figure 3b). In the both sampling years, two way ANOVA showed significant differences between the season (p < 0.001) but not between the stations.
Figure 3.
Seasonal and spatial variations in chlorophyll ‘b’ (a) for the sampling year 2011–2012 and (b) for the sampling year 2012–2013.
3.3. Chlorophyll ‘c’
In the year 2011–2012, the highest and lowest mean chlorophyll ‘c’ recorded in station 6 (PRM) and station 12 (POM) were 3.52 ± 2.3 and 0.96 ± 0.61 μg l−1, respectively. During the monsoon period, the maximum and minimum chlorophyll ‘c’ recorded was 2.81 ± 2.13 and 1.49 ± 0.83 μg l−1 respectively at station 13 and station 6 (Figure 4a). In the year 2012–2013, the highest and lowest mean chlorophyll ‘c’ recorded in station 6 (MON) and station 4 (PRM) were 5.34 ± 1.31 and 1.52 ± 1.11 μg l−1, respectively. During the post monsoon period, the maximum and minimum chlorophyll ‘c’ recorded was 3.91 ± 2.09 and 2.91 ± 1.31 μg l−1 respectively at station 7 and station 13 (Figure 4b). In the both sampling years, two way ANOVA showed significant differences between the season (p < 0.001) but not between the stations.
Figure 4.
Seasonal and spatial variations in chlorophyll ‘c’ (a) for the sampling year 2011–2012 and (b) for the sampling year 2012–2013.
3.4. Seasonal mean value chlorophyll ‘a, b, c’
The seasonal mean value for chlorophyll ‘a, b, c’ for the both sampling year was represented in Table 1. The seasonal mean value for chlorophyll ‘a’ varied between 1.07 ± 0.86 μg l−1 (MON) and 3.46 ± 2.05 μg l−1 (PRM) in the year 2011–2012. In the year 2012–2013, seasonal mean value fluctuated between 2.11 ± 1.83 μg l−1 (PRM) and 2.9 ± 1.47 μg l−1 (MON). The highest annual mean value 2.42 ± 1.46 μg l−1 was recorded in 2012–2013 and lowest annual mean value 1.98 ± 1.35 μg l−1 was recorded in the sampling year 2011–2012. In the year 2011–2012, the highest and lowest seasonal mean chlorophyll ‘b’ recorded in pre-monsoon and post monsoon was 2.76 ± 1.2 and 1.13 ± 1.07 μg l−1, respectively. In the year 2012–2013, the highest and lowest mean chlorophyll ‘b’ recorded in monsoon and post monsoon was 3.07 ± 1.33 and 2.07 ± 0.96 μg l−1, respectively. The highest and lowest annual average of chlorophyll ‘b’ was 2.35 ± 1.14 and 1.84 ± 1.6 μg l−1 in 2012–2013 and 2011–2012, respectively. In the year 2011–2012, the highest and lowest seasonal mean chlorophyll ‘c’ recorded in pre-monsoon and post monsoon was 2.80 ± 1.62 and 1.28 ± 1.02 μg l−1, respectively. In the year 2012–2013, the highest and lowest mean chlorophyll ‘c’ recorded in monsoon and pre monsoon was 3.59 ± 1.81 and 1.98 ± 1.21 μg l−1, respectively. The highest and lowest annual mean value of chlorophyll ‘c’ was 2.93 ± 1.41 and 2.08 ± 2.81 μg l−1 in 2012–2013 and 2011–2012, respectively.
Chlorophyll
Pre-monsoon (n = 112)
Monsoon (n = 112)
Post monsoon (n = 112)
Annual (n = 336)
2011–2012
2012–2013
2011–2012
2012–2013
2011–2012
2012–2013
2011–2012
2012–2013
Chlorophyll ‘a’ (μg l−1)
3.46 ± 2.056
2.11 ± 1.83
1.07 ± 0.86
2.9 ± 1.47
1.41 ± 1.11
2.24 ± 1.07
1.98 ± 1.35
2.42 ± 1.46
Chlorophyll ‘b’ (μg l−1)
2.76 ± 1.2
1.92 ± 1.14
1.63 ± 1.28
3.07 ± 1.33
1.13 ± 1.07
2.07 ± 0.96
1.84 ± 1.6
2.35 ± 1.14
Chlorophyll ‘c’ (μg l −1)
2.80 ± 1.619
1.98 ± 1.21
2.15 ± 1.75
3.59 ± 1.81
1.28 ± 1.02
3.22 ± 1.18
2.08 ± 2.81
2.93 ± 1.41
Table 1.
Analytical mean values (mean ± standard deviation) of chlorophyll a b c for the sampling years 2011–2012 and 2012–2013.
3.5. Microalgal diversity
Microalgae characteristics were given in Table 2. A total of 191 species of microalgae were identified from the 14 study sites along the Visakhapatnam offshore region, Bay of Bengal. Of these, 131 species were recorded under Ochrophyta division (68.58%) (127 species are diatoms (40.84% Centrales, 25.65% Pennales), 3 species belong to silicoflagellates 1.57% and 1 species in Eustigmatophyceae 0.52%), 35 species (18.3%) belong to dinophyta, 11 species (5.76%) belong to cyanophyta, 7 species (3.66%) belong to chlorophyta, 4 species (2.09%) belong to euglenozoa and 3 species (1.57%) belong to haptophyta. The community composition of phytoplankton was dominated by Centrales, which represented by 21 families with 33 genera. Chaetoceros (15 species), Bacteriastrum (5 species), Coscinodiscus and Thalassiosira (6 species) genus were encountered with more than five species and Rhizosolenia and Triceratium genus were recorded with four species under centric diatoms. Pennate diatoms were recorded with 30 genera and 49 species. In pennate diatoms, maximum four species were recorded under the same genus of Pleurosigma and three species were encountered in each genus of Nitzschia, Amphora, Diploneis and Fragilariopsis. In Dinophyta, 7 families and 10 genera were recorded, maximum number of species found in the genus were Neoceratium (8), Protoperidinium (8) Prorocentrum (3) and Dinophysis (4). In Cyanophyta, six families and eight genera were recorded. Trichodesmium, Lyngbya and Schizothrix genus were encountered with two species and remaining genera was observed with single species. In the division of Chlorophyta, 3 classes, 5 families and 5 genera were recorded, in this division species two species was encountered with Tetraselmis and Dunaliella genus. The division Haptophyta was observed with 3 species and 3 genera. In the Euglenozoa division, 2 genera with 4 species were noted and Silicoflagellates was observed with 2 genera and 3 species. During the course of study phytoplankton showed distribution pattern as: Centric diatoms>PennateDiatoms>Dinophyta>Cynophyta>Chloropyta>Euglenozoa>Haptophyta.
Division
Characteristics
Class
Family
Genus
Species
% of species
1. Ochrophyta
4
38
66
131
68.5
2. Dinophyta
1
7
10
35
18.3
3. Cyanophyta
1
6
8
11
5.76
4. Chloropyta
3
5
5
7
3.66
5. Euglenozoa
1
2
2
4
2.09
6. Haptophyta
2
2
3
3
1.57
Total
12
59
91
191
100
Centrales
1
21
33
78
40.84%
Pennales
1
15
30
49
25.65%
Dictyochaceae
1
1
2
3
1.57%
Monodopsidaceae
1
1
1
1
0.52
Table 2.
Characteristics of microalgae for the both sampling years.
3.5.1. Seasonal variations in qualitative abundance of microalgae
Seasonal variation in microalgae qualitative abundance was given in Table 3. Seasonal fluctuations of species composition in both sampling years varied from 150 (MON) to 161 (PRM). The species from phylum Ochrophyta fluctuated between 103 (PRM 2012–2013) and 115 (PRM 2011–2012). Centric diatoms varied between 66 (POM) and 70 (PRM) in the total of 78. Pennate diatoms ranged from 33 (MON) to 42 (PRM) from the total of 46. Dinoflagellate showed the variation between 23 (PRM) and 28 (MON) species in the total of 35. Cyanobacteria found to be low in post monsoon (8) and high during pre-monsoon (11) in the total of 11. In the division of haptophyta, species abundance fluctuated between 2 and 3 during pre-monsoon and monsoon, respectively and chlorophyta species ranged from 4 to 7 during monsoon and pre-monsoon, respectively.
Division
2011–2012
2012–2013
PRM
MON
POM
PRM
MON
POM
1. Ochrophyta
115
106
107
110
103
107
2. Dinophyta
23
27
26
26
28
28
3. Cyanophyta
11
9
9
10
9
8
4. Chlorophyta
6
7
6
7
4
5
5. Euglenozoa
4
3
2
3
3
3
6. Haptophyta
2
3
2
2
3
2
Total
161
155
154
158
150
153
Centrales
69
68
68
70
67
66
Pennales
42
36
38
37
33
38
Dictyochaceae
3
1
2
2
2
2
Monodopsidaceae
1
1
1
1
1
1
Table 3.
Quality abundance of microalgae.
3.5.2. Seasonal variations in qualitative abundance of microalgae
Checklists (species composition) of microalgae on the Visakhapatnam offshore region were summarized in Appendix A. The samples of all station were pooled for seasonal distribution analysis into samples of three season (pre-monsoon, monsoon and post monsoon) which helped in obtaining information about the distribution and species composition (or) diversity of the sea surface water. Abundance of phytoplankton during the study period (191 species) was reported along the Visakhapatnam Coast throughout the sampling years. Six divisions of microalgae Ochrophyta, Dinophyta, Cyanophyta, Chlorophyta, Euglenozoa and Haptophyta were recorded. The class Bacillariophyceae and Coscinodiscophyceae comprised of 36 families, 63 genera and 124 species. Altogether 191 species of microalgae belonging to the classes of Bacillariophyceae, Coscinodiscophyceae, Dinophyceae, Euglenophyceae, Chlorodendrophyceae, Chlorophyceae, Eustigmatophyceae, Dictyophyceae, Prymnesiophyceae, Pavlovophyceae, Trebouxiophyceae and Cyanophyceae were identified. Of these, Tetraselmis gracilis, Dicrateria inornata, Thalassiosira subtilis, Chaetoceros muelleri, Chaetoceros diversus, Skeletonema costatum, Thalassiosira subtilis and Asterionella inflata were considered important species based on the order of their abundance and frequency of occurrence. Present study indicates that the diatoms are the dominant group followed by the dinoflagellates and others.
Microalgal diversity is extremely important to analyze the status of an ecosystem. Local microalgae species have a competitive advantage under the local geographical, climatic and ecological conditions [16]. The levels of chlorophyll are the proof of photosynthetic activities and there was a distinct seasonality observed in the levels of phytoplankton biomass at the study sites. Total phytoplankton representing the maximum concentration of chlorophyll ‘a’ was recorded along the Visakhapatnam Coast during pre-monsoon (2011–2012) and monsoon (2012–2013). Surface phytoplankton abundance (as chlorophyll ‘a’ concentration) levels, reached up to 4.81 μg l−1, occurred in pre-monsoon, with the further smaller peaks in post monsoon and monsoon periods of the sampling year 2011–2012. In the year 2012–2013, peak chlorophyll levels reached up to 3.76 μg l−1, in monsoon. Highest chlorophyll ‘a’ concentration was reported during pre-monsoon (2011–2012) coincided with the period of upwelling and in the year 2012–2013, and the monsoon coincided with large scale mixing between surface river waters and deeper nutrient rich bottom waters [17]. The annual average (13.4 μg l−1) chlorophyll ‘a’ was reported for the entire euphotic zone of EEZ of Arabian Sea and it ranged from 0.1 to 96.4 μg l −1 [18]. Chlorophyll ‘a’ was found between 3.31 and 99.12 μg l −1 in surface water off Gopalpur, East coast of India, Bay of Bengal [19] and varied between 0.21 and 30.82 μg l −1 off Mangalore, West Coast of India [20] and the highest value 8.28 μg l −1 was observed during the post monsoon off Cape Comorin [21]. In the present work, the higher concentration of chlorophyll ‘b’ was recorded during monsoon, 2012–2013 and the West coast also recorded the highest concentration of chlorophyll ‘b’ (20.41 μg l −1) during the monsoon [22]. Relatively high chlorophyll ‘b’ indicates the presence of ultra or nano-planktonic microalgae coming under the class Chlorophyceae/Euglenophyceae/Prochlorophyceae [23].
Chlorophyll ‘c’ was recorded in lower concentrations during the pre and post monsoon season (2012–2013) and the same pattern was also observed in West Coast of India [22]. Lowest chlorophyll and DO concentrations were recorded during pre-monsoon period (2012–2013) has clearly indicated that plankton growth during pre-monsoon was reduced because of oxygen demand by the organic matter in the period of May and June [24]. The highest seasonal average of chlorophyll ‘a’, ‘b’ and ‘c’ in all stations were recorded during the monsoon of 2012–2013 due to the nutrient rich land runoff water in to the coastal areas. Monsoon rains and associated land runoff and nutrient loading determines the balance of organic to inorganic loadings which act as major factors controlling community responses of microalgae [25].
Earlier studies have reported that the nutrient supply could have significant effect on community composition of phytoplankton [26]. The nitrogen limitation is known to have a significant effect on phytoplankton composition. To determine the growth of phytoplankton, nutrients are the primary factors. The highly seasonal nature of monsoon rains might have increased the concentration nutrients (nitrite, nitrate and silicate) in monsoon period. The recorded low values during pre- and post-monsoon period may be due to its utilization by phytoplankton as evidenced by high photosynthetic activity and the dominance of neritic seawater having a negligible amount of nitrate [27]. In East Coast of India, Bay of Bengal a total number 249 species of phytoplankton comprising of 131 species of dinoflagellates, 111 species of diatoms and 7 species of cyanobacteria were recorded during 2004 [28]. In 2012, EEZ micro algae distributed studies reported 71 species, 30 genera with 22 families under Bacillariophyceae and 88 species of dinoflagellates encountered with 22 genera and 18 families [29]. In Cyanophyceae, 3 genera with 4 species and in Dictochophyceae one species were also reported in their studies.
Microalgal population (Diatoms>Dinoflagellates>Cyanophyta>Chlorophyta) pattern of this record was similar to that reported from Coastal waters off Rushikulya estuary, East Coast of India [30, 31]. Eurythermal and euryhaline nature of diatoms in all the three season leads to their dominance [32] and have been observed to bloom regularly along the Indian Coast during June to October [33, 34]. Dominance of diatom over dinoflagellates coincides to the report from Indian coastal water [35, 36, 37] and world oceans [38, 39]. A cosmopolitan genus such as Chaetoceros was dominant with 15 species followed by other major genera such as Coscinodiscus (6 species), Nitzschia (3 species) and Rhizosolenia (4 species) in this study. Oscillatoriaceae and Phormidiaceae were the dominant family in Cyanophyceae class with 3 species and followed by Schizotrichaceae (2 species) and the trend was similar to the studies in the same coast [40, 41]. Epiphytic cyanobacteria, Dactylococcopsis and Synechococcus and 17 genera belonging to chlorophyceae including Oocystis, Chlorella vulgaris was recorded in Palk Strait [42] and a total 44 species of Cyanobacteria from Tamilnadu [43] and Kerala [44].
Seasonal succession of phytoplankton population indicated that dinoflagellates mainly Neoceratium and protoperidinium as well as silicoflagellates; Dictyocha were dominant population in pre-monsoon periods. In diatoms Rhizosolenia, Guinardia, Thalassiosira, Chaetoceros and Asterionella genus were relatively more dominantly throughout the sampling years. The same sequences in the phytoplankton abundance were reported at East Coast India [45] and from Pakistan 15 species in Navicula was reported [46]. Generally, Skeletonema costatum found to be dominant in this Coast coincides was agree with earlier studies [45, 47, 48]. Chaetoceros species have contributed high in total population of centric diatoms and it is coincided by the findings during pre-monsoon periods [45, 49]. In Sundarbans also diatom reported as dominant group in the overall phytoplankton group and Skeletonema and Chaetoceros are more abundant species East Coast of India [50].
In the current study, genus Asterionella and Fragilariopsis were observed throughout the year but the highest numbers were observed in the months of April and June 2013. Asterionella japonica bloom and discoloration Off Waltair, East coast [51] and north western Bay of Bengal [52] strongly supporting our findings. Pleurosigma species with five different classes was reported as dominant species in Nizampatnam, East Coast of India [53]. The maximum numbers of diatoms recorded in post monsoon period was only five numbers more than that of pre-monsoon. In waters off Visakhapatnam Coast, some genera of green algae under the division of Chlorophyta i.e. Chlorella, Tetraselmis, and Dunaliella were recorded throughout in both samplings years. Prasinophyceae and Trebouxiophyceae classes were found predominantly along with some prokaryotic and eukaryotic picoplankton species in the same Coast [54]. Highest number of species under chlorophyta was recorded during pre-monsoon periods like cyanophyceae. In contrary, Southwest Coast of India had recorded that the cyanophycean and chlorophycean species distributed during the monsoon period of the years 2006–2008 [22]. As like as centric diatoms, cyanophyta also showed a maximum number of species during pre-monsoon period, however, the maximum values were obtained for dinoflagellates during monsoon period. The annual mean water temperature 29°C for both sampling years supported for the abundance of flagellates throughout the year. The abundance of flagellate species was commonly occurring at higher water temperatures [55]. In the Visakhapatnam coastal waters, Haptophyceae and Prasinophyceae classes were most abundant [56] and 17 species of flagellates represented by 6 diverse groups in the same coastal waters [57]. Dictyocha fibula was recorded during monsoon periods of both the sampling years except during post monsoon (2011–2012) and the same sequence were reported the lower abundance of oceanic species Dictyocha fibula during pre- and post-monsoon [58].
Chlorophyll concentrations and diversity of microalgae in Visakhapatnam offshore region studied in detail for a period of 2 year (2011–2012 and 2012–2013). Our results revealed that the diatoms were found to be dominant with number of species in Visakhapatnam offshore waters, Bay of Bengal. From this study, we had found the suitable spatial and season to get sea water to isolate particular species of microalgae and which is use full for shrimp hatchery in that coastal zone. And another advantages of this study was pin point spatial of this coastal area may help to isolate microalgae can be grown in open pond without any major contamination to produce biomass for biodiesel production.
The authors are grateful to the Council for Scientific and Industrial Research (CSIR), Government of India, New Delhi, India. This study was undertaken as a part of CSIR-NMITLI project (grant no.: 5/258/64/2009 — NMITLI; date: 19-04-2010) on “Biofuel from marine microalgae” co-funded by the Ministry of Earth Sciences (MoES).
Phylum: Cyanobacteria (Stanier ex Cavalier-Smith, 2002)
Class: Cyanophyceae (Schaffner, 1909)
2011–2012
2012–2013
S. No
Family
PRM
MON
POM
PRM
MON
POM
1
Oscillatoriaceae (Engler, 1898)
1
Lyngbya majuscule (Harvey ex Gomont, 1892)
+
—
+
+
—
+
2
Lyngbya confervoides (Agardh ex Gomont, 1893)
+
+
+
+
+
+
3
Oscillatoria princeps (Vaucher ex Gomont, 1892)
+
+
+
+
+
—
2
Phormidiaceae (Anagnostidis & Komarek, 1988)
4
Phormidium nigroviride (Thwaites ex Gomont) Anagnostidis & Komarek, 1988
+
+
—
+
+
—
5
Trichodesmium thiebautii (Gomont, 1892)
+
+
+
+
+
+
6
Trichodesmium erythraeum (Ehrenberg ex Gomont, 1893)
+
+
+
+
+
+
3
Nostocaceae (Eichler, 1886)
7
Anabaenopsis elenkinii (Miller, 1923)
+
—
+
+
+
+
4
Schizotrichaceae (Elenkin, 1949)
8
Schizothrix calcicola (Agardh) Gomont ex Gomont, 1892
+
+
+
+
—
+
9
Schizothrix fuscescens (Kutzing, 1843)
+
+
+
+
+
+
5
Synechococcaceae (Nageli, 1849)
10
Synechococcus sp. (Nageli, 1849)
+
+
—
—
+
+
6
Chroococcaceae (Hansgirg, 1888)
11
Dactylococcopsis sp. (Hansgirg, 1888)
+
+
+
+
+
—
Class 1, Families 6, Genera 8 and Species 11.
11
9
9
10
9
8
Phylum: Euglenozoa (Cavalier-Smith, 1981)
Species
2011–2012
2012–2013
Family
PRM
MON
POM
PRM
MON
POM
Class 1: Euglenophyceae (Schoenichen, 1925)
1
Eutreptiaceae (Hollande, 1942)
1
Eutreptia lanowii (Steuer, 1904)
+
+
—
—
+
+
2
Eutreptia viridis (Perty, 1852)
+
+
—
+
—
+
2
Euglenaceae (Dujardin, 1841)
3
Euglena proxima (Dangeard, 1901)
+
—
+
+
+
—
4
Euglena ascusformis (Schiller, 1925)
+
+
+
+
+
+
Class 1, Families 2, Genera 2, Species 4
4
3
2
3
3
3
Phylum: Chlorophyta (Pascher, 1914)
Family
Class 1: Chlorodendrophyceae (Massjuk, 2006)
1
Chlorodendraceae (Oltmanns, 1904)
1
Tetraselmis gracilis (Kylin) Butcher, 1959
+
+
+
+
—
+
2
Tetraselmis chui (Butcher, 1959)
+
+
+
+
+
—
Class 2: Chlorophyceae (Wille in Warming, 1884)
2
Dunaliellaceae (Christensen, 1967)
3
Dunaliella tertiolecta (Butcher, 1959)
+
+
+
+
+
+
4
Dunaliella salina (Dunal) Teodoresco, 1905
+
+
+
+
+
+
3
Chlamydomonadaceae (Stein, 1878)
5
Chlamydomonas reinhardtii (Dangeard, 1888)
—
+
+
+
—
+
Class 3: Trebouxiophyceae (Friedl, 1995)
4
Chlorellaceae (Brunnthaler, 1913)
6
Chlorella vulgaris (Beijerinck, 1890)
+
+
+
+
+
+
5
Oocystaceae (Bohlin, 1901)
7
Oocystis solitaria (Wittrock, 1879)
+
+
—
+
—
—
Class 3, Families 5, Genera 5 and Species 7.
6
7
6
7
4
4
Phylum: Haptophyta (Cavalier-Smith, 1986)
Class: Prymnesiophyceae (Hibberd, 1976)
1
Prymnesiaceae (Conrad ex O.C.Schmidt, 1931)
1
Dicrateria inornata (Parke, 1949)
+
+
+
+
+
+
2
Isochrysis galbana (Parke, 1949)
+
+
—
+
+
+
Class: Pavlovophyceae (Cavalier-Smith) Green & Medlin in Edvardsen et al., 2000
2
Family: Pavlovaceae (Green, 1976)
3
Diacronema lutheri (Droop) Bendif & Veron, 2011
—
+
+
—
+
—
2 Family, 2 Class, 3 Genera and 3 Species
2
3
2
2
3
2
References
1.Raman AV. Pollution effects in Visakhapatnam harbour, India: An overview of 23 years of investigations and monitoring. Helgoländer Meeresuntersuchungen. 1995 Mar 1;49(1):633
2.Renuka N, Sood A, Prasanna R, Ahluwalia AS. Phycoremediation of wastewaters: A synergistic approach using microalgae for bioremediation and biomass generation. International Journal of Environmental Science and Technology. 2015 Apr 1;12(4):1443-1460
3.Sultana R, Rao DP. Bioaccumulation patterns of zinc, copper, lead, and cadmium in grey mullet, Mugil cephalus (L.), from harbour waters of Visakhapatnam, India. Bulletin of Environmental Contamination and Toxicology. 1998 Jun 24;60(6):949-955
4.Thornton KW, Kimmel BL, Payne FE, editors. Reservoir Limnology: Ecological Perspectives. Canada: John Wiley & Sons; 1990 May 14
5.Cody ML. Introduction to Long-Term Community Ecological. Long-Term Studies of Vertebrate Communities. Nature. San Diego: Academic Press; 24 Oct 1996. 597 p
6.Rosen BH. Microalgae Identification for Aquaculture. Florida: Florida Aqua Farms; 1990
7.Hallegraeff GM, McCausland MA, Brown RK. Early warning of toxic dinoflagellate blooms of Gymnodinium catenatum in southern Tasmanian waters. Journal of Plankton Research. 1995 Jun 1;17(6):1163-1176
8.Tomas CR, editor. Identifying Marine Phytoplankton. Science. San Diego: Academic Press, Elsevier; 12 Aug 1997. 858 p
9.Hällfors G. Checklist of Baltic Sea Phytoplankton Species (Including some Heterotrophic Protistan Groups). Helsinki Commission, Issue 95 of Baltic Sea environment proceedings, Finnish Institute of Marine Research, Finland; 2004. ISSN 0357-2994
10.Manal AK, Faiza AY, Kholood AR. Marine Phytoplankton Atlas of Kuwaits Waters. Safat, Kuwait: Kuwait Institute for Scientific Research; 2009:99906-99941
11.Achary MS, Sahu G, Mohanty AK, Samatara MK, Panigrahy SN, Selvsnsysgam M, Satpathy KK, Prasad MV, Panigrahy RC. Phytoplankton abundance and diversity in the coastal waters of Kalpakkam, east coast of India in relation to the environmental variables. Bioscan. 2010;2:553-568
12.Tsarenko PM. Trebouxiophyceae. In: Tsarenko PM, Wasser SP, Nevo E, editors. Algae of Ukraine: Diversity, Nomenclature, Taxonomy, Ecology and Geography. Chlorophyta. California: A.R.A. Gantner; 2006;3:61-108
13.Pham MN, Tan HT, Mitrovic S, Yeo HH. A Checklist of the Algae of Singapore. Singapore: Raffles Museum of Biodiversity Research, National University of Singapore; 2011
14.Komarek J, Kastovsky J, Mares J, Johansen JR. Taxonomic classification of cyanoprokaryotes (cyanobacterial genera) 2014, using a polyphasic approach. Preslia. 2014 Dec 1;86(4):295-335
15.Varol M, Sen B. Dicle Nehrinin Planktonik Alg floras [Flora of the Planktonic algae of the Tigris River]. Journal of Fisheries Sciences. 2014;8:1-4
16.Selvakumar P, Umadevi K. Mass cultivation of marine micro alga Nannochloropsis gaditana KF410818 isolated from Visakhapatnam offshore and fatty acid profile analysis for biodiesel production. Journal of Algal Biomass Utilization. 2014;5:28-37
17.Ganapati PN. Biological oceanography of the Bay of Bengal. Mahasagar. 1973 Jan 1;6(2):84-94
18.Sarupria JS, Bhargava RM. Seasonal distribution of chlorophyll-a in the exclusive economic zone (EEZ) of India. Indian Journal of Marine Sciences. 1998;(27):292-297
19.Panigrahy RC, Mishra S, Sahu G, Mohanty AK. Seasonal distribution of phytoplankton in the surf waters off Gopalpur, east coast of India. Journal of the Marine Biological Association of India. 2006;48(2):156-160
20.Lingadhal C, Gupta TR, Ramesh AM. Distribution of chlorophyll pigments in the Arabian Sea off Mangalore (west coast of India) in relation to nutrients. Journal of Marine Biological Association of India. 2003;46:126-132
21.Gopinathan CP, Gireesh R, Smitha KS. Distribution of chlorophyll‘a’and'b’in the eastern Arabian Sea (west coast of India) in relation to nutrients during post monsoon. Journal of the Marine Biological Association of India. 2001;43(1 & 2):21-30
22.Sanilkumar MG. Microalgae in the southwest coast of India [PhD thesis]. India: Department of Marine Biology, Microbiology and Biochemistry Cochin University of Science and Technology Kochi; February, 2009
23.Hoek C, Mann D, Jahns HM. Algae: An Introduction to Phycology. Melbourne, Australia: Cambridge University Press; 1995
24.Sarma VV, Raju GR, Babu TB. Pollution characteristics and water quality in the Visakhapatnam harbour. Mahasagar. 1982 Jan 1;15(1):15-22
25.Hopkinson CS, Vallino JJ. The relationships among man’s activities in watersheds and estuaries: A model of runoff effects on patterns of estuarine community metabolism. Estuaries and Coasts. 1995 Dec 1;18(4):598-621
26.Riebesell U. Effects of CO2 enrichment on marine phytoplankton. Journal of Oceanography. 2004 Aug 1;60(4):719-729
27.Bragadeeswaran S, Rajasegar M, Srinivasan M, Rajan UK. Sediment texture and nutrients of Arasalar estuary, Karaikkal, south-east coast of India. Journal of Environmental Biology. 2007 Apr 1;28(2):237-240
28.Madhav VG, Kondalarao B. Distribution of phytoplankton in the coastal waters of east coast of India. Indian Journal of Marine Sciences. 2004;33(3):262-268
29.Harikhrishnan E. Marine microalgae in the EEZ of India [PhD thesis]. Department of Marine Biology, Microbiology and Biochemistry, Science and Technology. Kerala, India: Cochin University; Feb 2012
30.Gouda R, Panigraphy RC. Diurnal variation of phytoplankton in Rushikulya estuary, east coast of India. Indian Journal of Marine Sciences. 1989;18(4):246-250
31.Prabhahar C, Saleshrani K, Enbarasan R. Studies on the ecology and distribution of phytoplankton biomass in Kadalur coastal zone Tamil Nadu, India. Current Botany. 2011;2(3):26-30
32.Naik S, Acharya BC, Mohapatra A. Seasonal variations of phytoplankton in Mahanadi estuary, east coast of India. Indian Journal of Marine Sciences. 2009;38(2):184-190
33.Ramamurthy VD, Selvakumar RA, Bhargava RM. Studies on the blooms of Trichodesmium erythraeum (Ehr.) in the waters of the central west coast of India. Cur Sci. 1972:803-805
34.Devassy VP, Bhattathiri PM. Phytoplankton ecology of the cochin backwaters. Indian Journal of Geo-Marine Sciences. 1974;3(1):46-50
35.Huang L, Jian W, Song X, Huang X, Liu S, Qian P, Yin K, Wu M. Species diversity and distribution for phytoplankton of the Pearl River estuary during rainy and dry seasons. Marine Pollution Bulletin. 2004 Oct 31;49(7):588-596
36.Choudhury AK, Pal R. Phytoplankton and nutrient dynamics of shallow coastal stations at Bay of Bengal, eastern Indian coast. Aquatic Ecology. 2010 Mar;44(1, 1):55-71
37.Sahu G, Satpathy KK, Mohanty AK, Sarkar SK. Variations in community structure of phytoplankton in relation to physicochemical properties of coastal waters, southeast coast of India. Journal of Geo-Marine Sciences. 2012;41(3):223
38.Chisholm SW. Oceanography: Stirring times in the Southern Ocean. Nature. 2000 Oct 12;407(6805):685-687
39.Palleyi S, Kar RN, Panda CR. Seasonal variability of phytoplankton population in the Brahmani estuary of Orissa, India. Journal of Applied Sciences and Environmental Management. 2008;12(3):19-23
40.Desikachary TV. Cyanophyta. Monographs on Algae. New Delhi, India: Indian Council of Agricultural Research; 1959
41.Thajuddin N, Subramanian G. Cyanobacterial biodiversity and potential applications in biotechnology. Current Science. 2005 Jul;10:47-57
42.Govindasamy C, Anantharaj K, Srinivasan R, Gavaskar K. Epiphytic cyanobacteria linked on Molluscs in Palk Strait, Tamilnadu, India. American-Eurasian Journal of Scientific Research. 2013;8(4):188-191
43.Muthukumar C, Muralitharan G, Vijayakumar R, Panneerselvam A, Thajuddin N. Cyanobacterial biodiversity from different freshwater ponds of Thanjavur, Tamilnadu (India). Acta Botanica Malacitana. 2007;32:17-25
44.Joseph S, Saramma AV. Seasonal and spatial distribution of cyanobacteria in Cochin estuary and nearshore waters. In: Proceedings of MB R 2004 National Seminar on New Frontiers in Marine Bioscience Research; 2004. pp. 459-468
45.Latha TP, Rao KH, Amminedu E, Nagamani PV, Choudhury SB, Lakshmi E, Sridhar PN, Dutt CB, Dhadwal VK. Seasonal variability of phytoplankton blooms in the coastal waters along the east coast of India. The International Archives of Photogrammetry, Remote Sensing and Spatial Information Sciences. 2014 Jan 1;40(8):1065
46.Khan ZU, Mahmood S, Khan RU, Khan SU. Diversity of Navicula (Bacillariophyceae) in the area of district bannu, Khyber Pakhtoonkhwa, Pakistan. Journal of Algal Biomass Utilization. 2013;4(3):46-49
47.Subba Rao DV. Marine plankton diatoms as indicators of ocean circulation in the Bay of Bengal. Botanica Marina. 1976;19(3):181-184
48.Paul JT, Ramaiah N, Gauns M, Fernandes V. Preponderance of a few diatom species among the highly diverse microphytoplankton assemblages in the Bay of Bengal. Marine Biology 2007 Jul 1;152(1):63-75
49.Ayajuddin M, Pandiyarajan RS, Ansari ZA. Distribution of phytoplankton along an environmental gradient off Kakinada, East Coast of India. Indian Journal of Geo-Marine Sciences. 2014;43(3):357-364
50.Mitra A, Zaman S, Sett S, Raha AK, Banerjee K. Phytoplankton cell volume and diversity in Indian Sundarbans. Indian Journal of Geo-Marine Sciences. 2014;43(2):208-215
51.Rao DS. Asterionella japonica bloom and discoloration off Waltair, Bay of Bengal. Limnology and Oceanography. 1969 Jul 1;14(4):632-634
52.Sasamal SK, Panigrahy RC, Misra S. Asterionella blooms in the northwestern Bay of Bengal during 2004. International Journal of Remote Sensing. 2005 Sep 10;26(17):3853-3858
53.Pandiyarajan RS, Shenai-Tirodkar PS, Ayajuddin M, Ansari ZA. Distribution, abundance and diversity of phytoplankton in the inshore waters of Nizampatnam, South East Coast of India. Journal of Geo-Marine Sciences. 2014;43(3):348-356
54.Chari NV, Rao PS, Sarma NS. Fluorescent dissolved organic matter in the continental shelf waters of western Bay of Bengal. Journal of Earth System Science. 2013 Oct 1;122(5):1325-1334
55.Lehman PW. The influence of climate on phytoplankton community biomass in San Francisco Bay estuary. Limnology and Oceanography. 2000 May 1;45(3):580-590
56.Phaniprakash K. Phytoplankton ecology in relation to pollution in Visakhapatnam harbour, Bay of Bengal [doctoral dissertation thesis]. Waltair: Andhra University; 1989
57.Bharati VR, Kalavati C, Raman AV. Planktonic flagellates in relation to pollution in Visakhapatnam harbour, East Coast of India. Indian Journal of Marine Sciences. 2001;30(1):25-32
58.Thomas MA. Algal blooms and associated bacteria along the Southwest Coast of India [doctoral dissertation]. Kerala: Cochin University of Science and Technology; 2014
Written By
Palanisamy Selvakumar, Athia Shameem, Katru Umadevi, Boddu
Sivaprasad and Ajith Haridas
Reviewed: February 16th, 2018Published: June 27th, 2018
Open access peer-reviewed chapter
