Open access peer-reviewed chapter

Environmental Influence of Soil toward Effective Vermicomposting

Written By

Jaswinder Singh, Sharanpreet Singh, Adarsh Pal Vig and Arvinder Kaur

Submitted: October 20th, 2017 Reviewed: February 9th, 2018 Published: June 27th, 2018

DOI: 10.5772/intechopen.75127

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Abstract

Vermicomposting is a process by which the organic waste is converted into manure with the help of earthworms. Growth rate, onset of maturity (clitellum development), rate of reproduction (cocoon production) and population buildup of earthworm during vermicomposting have been depend upon the conditions like temperature, moisture and physico-chemical properties of the feed mixtures. Eisenia fetida was superior to other epigeic species and tolerate wide range of temperature, moisture and pH. Endogeic species produced lesser cocoon than epigeic species and cocoon production decreased at low temperature. Maintenance of temperature and moisture content is the critical step for vermicomposting. Growth and maturation of earthworms was best at 20–25°C temperature with 80–85% moisture content. Increase temperature upto 30°C accelerated growth rate of earthworms and lessened the time to sexual maturity. Earthworms can survive in the soil contaminated with heavy metals by accumulating heavy metals in their tissues.

Keywords

  • earthworm
  • growth rate
  • physico-chemical properties
  • heavy metals
  • vermicomposting

1. Introduction

Over the past few years disposal and management of organic solid wastes has become more problematic and rigorous due to rapidly increasing population, intensive agriculture and industrialization. Aristotle, the Greek philosopher, documented very early the importance of earthworm in the ecosystem, and called them “intestines of the earth”. After that, Darwin [1] highlighted the role of earthworm in breakdown of animal matter as well as dead plant. Transformation of organic waste through vermicomposting is of multifold interest as along with checking pollution of the environment, it results in production of a rich, more stable and homogenous product as compared to composting [2, 3]. Vermicompost acts as a buffer, has a significantly lower volatile solid content and high N, P, K content in the plant available form [4, 5].

Out of the 3200 species of earthworm reported from the world over, 509 species belonging to 67 genera are known to occur in the Indian subcontinent and about 374 species have been reported from different habitats of India alone [6, 7]. Certain epigeic earthworms, which have natural ability to colonize organic waste; show tolerance to wide range of environmental factors; short life cycle; high reproductive rates; high rates of feedstock consumption, digest and assimilate organic matter shows good potential for vermicomposting [8]. Few surface feeders earthworms species which contain all these characteristics and widely used in vermicomposting of a wide variety of organic wastes are Eisenia fetida(Savigny), Eudrilus eugeniae(Kinberg), Eisenia andrei(Bouche), Dendrobaena veneta(Rosa) and Perionyx excavatus(Perrier). Other species like Dichogaster modigilani, Drawida nepalensis, Lampito mauritii, Lumbricus rubellusand Dendrobaena rubidahave also been used to some extent [9, 10]. Growth rate, onset of maturity (clitellum development), rate of reproduction (cocoon production) and population buildup were recorded as the indices of suitability of a particular mixture for the worms.

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2. Suitable species of earthworm used for vermicomposting

Epigeics species are useful for biosolid waste management as these worms can hasten the composting process to a significant extent and produce better quality of vermicomposts, compared with those prepared through traditional methods [11]. Eisenia. fetidais used throughout the world for this purpose as it is ubiquitous, can tolerate a wide range of temperature and can live in wastes with good moisture content [8, 12]. Eudrilus eugeniaeand Perionyx excavatusis the other commonly used worm. Eudrilus eugeniaeis large in size, grows rapidly but has poor temperature tolerance, hence, may be suitably used in the areas with less fluctuation of temperature (tropical areas).

Lampito mauritii, Drawida willsi, Dichogaster bolaniand Pheretima elongataare some others useful species used in the vermicomposting [13, 14, 15, 16]. There is a species specific variation in food preference and accordingly the time taken for bioremediation also varies. Kaviraj and Sharma [16] compared the efficiency of exotic Eisenia fetidaand local Lampito mauritiiin vermicomposting of municipality solid waste and showed that Eisenia fetidawas much better in vermicomposting as compare to Lampito mauritiiin terms of C:N ratio reduction, TOC reduction, increase in EC and TK but Lampito mauritiiwas able to modify the soil characteristics. Tripathi and Bhardwaj [11] also observed that both the species of earthworms increased the N, P, K content while decreased the C:N and C/P ratios after 150 days. Moreover, the average number of hatchlings and cocoons produced by Eisenia fetidawere also much more than Lampito mauritii. Thus, they concluded that Eisenia fetidawas a better adapted species for vermicomposting of kitchen waste mixed with cow dung under tropical conditions. Dominguez et al. [17] found that Eudrilus eugeniaeis also be a suitable species for vermicomposting. Out of the Indian species Perionyx excavatus, Dichogaster modigilani, Drawida nepalensisand Lampito mauritiicould be exploited for vermicomposting because of their continuous breeding, high rate of cocoon production, short development time and high hatching success [18].

Earthworm species Eudrilus eugeniae, Eisenia fetida, Perionyx sansibaricus, Pontoscolex corethrurusand Megascolex chinensiswere compared for their efficiencies in biodegrading organic wastes and Eudrilus eugeniaewas found to be superb of all these [10]. Eudrilus eugeniaeinoculated in waste also diminish the toxic heavy metals and might be helpful in obtaining a clean environment [19]. Some scientists recommend that vermicomposting with polyculture gives faster results over monoculture [13, 20]. When three earthworm species, i.e. Eisenia fetida, Perionyx excavatus(epigeic) and Lampito mauritii(anecic) were used, the reactor with polyculture performed better than the traditional monoculture vermireactors [21]. On the other hand Elvira et al. [22] found that mixed cultures of epigeics species viz. L. rubellus, E. fetidaand D. rubidadid not show any advantage over pure cultures. They reported that E. andreishowed higher growth rates in mixed cultures while the growth rate of L. rubellusand D. rubidadecreased slightly in mixed cultures as compared to pure cultures.

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3. Effect of temperature and moisture on earthworm

Reinecke and Kried [23] and Reinecke and Venter [24] concluded that E. fetidacould survive well even in harsh environmental conditions, especially temperature (5–43°C) and fluctuating moisture conditions. However, growth and maturation of earthworms was best at 20°C and 85% moisture content under laboratory conditions [11, 25]. Dominguez and Edwards [26] found that 80% moisture was optimum for vermicomposting of pig manure and hostel kitchen waste by Eisenia anderi. Lalander et al. [27] observed that if the temperature of the vermibed or unit was more than threshold temperature, then earthworm moves to the edges and top levels, leaving unprocessed material in the center.

Edwards et al. [28] studied the life cycle of Perionyx excavatusin a variety of organic wastes under various population density pressures and temperatures between 15 and 30°C. They observed that an increase in temperatures up to 30°C accelerated the growth of earthworms but decreased the time to sexual maturity. However, the highest rates of reproduction occurred at 25°C both in cattle solids and sewage sludge. Earthworms grew at similar rates in cattle solids, pig solids and aerobically digested sewage sludge, but the earthworms did not grow well in horse solids and grew poorly in turkey wastes. Edward [29] in another study evaluated the optimal conditions for breeding of E. fetidawith different range of animal and vegetable waste under aerobic condition with temperature ranged from 15–20°C, moisture 80–90%, ammonia content <0.5 mg/g, salt content <0.5% and pH in the range of 5–9. He found that the population density of earthworms per unit volume or weight of a waste was very important in affecting rate of growth and reproduction.

Dominguez et al. [17] made an observation on the biology and population dynamics of Eudrilus eugeniaein cattle waste solids by growing them in groups of 1, 2, 4, 8 or 16 hatchlings in 100 g of waste in incubators at 15, 20, 25 and 30°C. They found that earthworm biomass production was temperature dependent, maximum production being attained at the two highest population densities and highest temperature (30°C). The highest organic waste to earthworm ratio of 10:1 (10%) was recorded at the most dense earthworm population.

Bhattacharjee and Chaudhuri [9] studied the reproductive biology of seven Indian species of earthworm, viz. Lampito mauritiiKinberg, Polypheretima elongata(Perrier), Perionyx excavatusPerrier, Pontoscolex corethrurus(Muller), Dichogaster modiglianii(Rosa), Eutyphoeus gammiei(Beddard), and Drawida nepalensisMichaelsen in different seasons (natural variation in temperature). The peregrine earthworms such as Dichogaster modiglianii, Perionyx excavatus, Polypheretima elongataand Pontoscolex corethruruswere continuous breeders with high fecundity. Native Lampito mauritiiand Drawida nepalensiswere found to be semi-continuous, whereas, Eutyphoeus gammieiwas a discrete breeder. There was a dramatic increase in cocoon in the summer and monsoon months with peaks during April and July while cocoon production decreased during winter. Temperature also affected the incubation period of cocoons. With increase in temperature within a temperature range of 28–32°C under laboratory conditions, incubation period increased in the endogeic worms (Pontoscolex corethrurus, Polypheretima elongata and Drawida nepalensis) and decreased in the epigeic worms (Perionyx excavatusand Dichogaster modiglianii). There was a significant (P < 0.05) positive correlation between number of hatchlings per cocoon and incubation period in Lampito mauritiionly. Edwards and Arancon [30] reported that an increase in temperatures up to 30°C accelerated the growth of Perionyx excavatusand reduced the time for attaining sexual maturity. However, the highest rates of reproduction occurred at 25°C both in cattle solids and sewage sludge.

Elvira et al. [22] studied the growth rate and reproduction of the epigeic species Lumbricus rubellusand D. rubidain cow dung and their possible interactions with E. andrei. The mean growth rate of D. rubidawas 3.84 mg/day, reaching sexual maturity at 54 days and producing an average of 1.45 cocoons per week. After collection, 85% of the cocoons of this species were viable, incubation took an average of 21.7 days and an average of 1.67 worms emerged from each cocoon. On the other hand, the mean growth rate of L. rubelluswas 8.02 mg/day, maturity at 74 days, with a mean weekly production of 0.54 cocoons. After an incubation period of 36.5 days, 64% of the cocoons hatched and one worm emerged from each. The mixed cultures tested did not present any advantage over pure cultures; E. andreishowed higher growth rates in mixed cultures, while the growth rate of L. rubellusand D. rubidadecreased slightly in mixed cultures as compared to pure cultures. On the other hand, maximum biomass gain by P. excavatuswas 292 mg per gram cattle waste at 25°C. Increasing temperatures up to 30°C accelerated the growth of earthworms and lessened the time to sexual maturity [29].

Soobhany et al. [19] resulted that moisture content within the feed mixture decreased with an increase in temperature by employing E. eugeniaein municipal solid waste. Initially they set the starting temperature of municipal solid waste in the range of 28–30°C and moisture content within the range of 81.8–83%. During vermicomposting of municipal solid waste, a sharp increase in temperature was recorded by them which mainly due to heat evolution resulting from rapid breakdown of organic matter and nitrogenous compounds by microbial activities which cause the water evaporation from the feed mixture and moisture content was decreased upto 50–55%. So thus maintenance of temperature and moisture content is the critical step for vermicomposting.

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4. Growth rate of earthworm

The earthworms grow best in easily metabolizable organic matter and non-assimilated carbohydrates; these also favor their reproduction [31]. There was a positive correlation between Growth and reproduction with volatilable solid content of the waste [29]. Earthworm growth slows down when C: N ratio and temperature is high [32, 33]. The biomass gain by E. fetidaduring vermicomposting was found to depends on the population density and food type [34]. Viljoen and Reinecke [35] observed that single raised worm began to gain biomass at a higher rate than those raised in batches. While Dominguez et al. [36] reported a decrease in worm biomass even when additional feed was provided to worms every week. So the physico-chemical or the nutrient characteristics of the waste might be related to the growth of earthworm along with temperature, pH and moisture content. The interaction of these physico-chemical organic waste palatability and strength of feeding by earthworm is directly related to the interaction of these parameters and consequently it affects growth and reproduction of earthworm [37]. Hartenstein et al. [38] reported the regression equations for Eisenia fetidawith respect to its age and observed that 50% of the earthworm population became clitellate at 25°C in relation to population density in activated sludge and in horse manure. A mean particle size of 0.3 mm of horse manure proved superior in supporting a weight gain (+45%) than a particle size of 0.5 or 1 mm. Pure cellulose, newspaper or wood shavings as substrate were ingested by E. fetidabut failed to result in weight gain. Neuhauser et al. [39] and Neuhauser et al. [40] also reported a weight loss in E. fetidafor a longer duration in the waste. This might be due to the transformation of most of the substrate to vermicompost, which could not further support their growth. Gunadi et al. [41] reported that E. fetidaand E. anderigrew much faster in tea leaf wastes pre-composted for 1 week as compare to fresh waste because of the high protein content. However in fresh cattle solids, death of E. fetidawas observed after 2 weeks by Gunadi and Edward [42]. They attributed death of earthworms to the anaerobic conditions which developed after 2 weeks in fresh cattle solids. Mature worms were not able to adapt to the medium as compared to 20 day old worms. Rates of growth and cocoon production were slightly less in a defined medium (7% organic content) than in a cow manure control medium (70% organic content) [43].

Reinecke and Viljoen [44] observed that cocoon production by Eudrilus eugeniaewas much more at 25°C in different types of substrates. Evans and Guild [45] however, observed a peak production of 3.8 cocoons per week per individual of Eisenia fetidaat 13°C. On the other hand Venter and Reinecke [46] observed clitellum for the first time in Eisenia fetidaafter 60 days. A positive correlation was observed by Satchell [47] between number of cocoons and the zone of soil inhabited by worms. The species of the deeper soil layer produced less cocoons as compare to species living near the surface due to adverse environmental condition. Olive and Clark [48] reported that temperature more than optimum level decreased cocoon production in earthworms. Lavelle [49] found a positive relationship between the size of the adult and cocoons produced by the earthworms but Senapati and Sahu [50] reported that the size of worms bore a negative relationship with the number of cocoons. They asserted that greater rate of cocoon production by small to medium sized epigeic earthworm Dichogaster modiglianiiand Perionyx excavatusand top soil endogeic worms Pontoscolex corethrurusand Lampito mauritiiwas due to exposure to the high mortality risk environment. Lee [51] and Edwards and Bohlen [52] proposed that size of cocoon was not always correlated with size of worms as cocoon production and time for maturation varied with species, population density and external factors such as temperature, moisture and energy content of the available food. Barne and Striganova [53] noticed higher mortality rate with increase in density of worms but Jager et al. [54] reported that growth curves were hardly affected by changing the density of earthworm Eisenia venetabut had an unexpected effect on reproduction. At higher density, the earthworm produced cocoons at larger body size and the maximum reproduction rate was lower. Reinecke and Viljoen [44] found no significant correlation between cocoon size and number of hatchlings produced. Hatching success of cocoon produced by worms younger than 60 days was low, the rate of hatching increased as the worms grew. Gunadi et al. [55] reported that the numbers of cocoons were less with increasing time of pre-composting but there was no clear pattern of effect of pre-composting on the number of hatchlings produced in cattle solids. Weight of hatchlings varied from 2.5 to 2.6 mg/cocoon.

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5. Changes in physico-chemical quality of the feed wastes during vermicomposting

The physio-chemical composition of the vermicompost is known to be influenced by the different kind of feed given to the animal, bedding material used and the way the waste is collected, stored and handled before utilization [56]. A detailed review of various changes in physico-chemical parameters of feed material during vermicomposting is given in the following section.

5.1. pH and electrical conductivity (EC)

The differences in the pH of vermicompost are directly dependent on the type of raw materials used for vermicomposting. Different substrates used for vermicomposting resulted in different types of intermediates products which shows a different behavior in pH shift. The neutral pH throughout the vermicomposting is ideal for the growth of earthworm [57]. The occurrence of acidic environment may be attributed to the bioconversion of organic acids or higher mineralization of the nitrogen and Phosphorus into nitrites /nitrates and orthophosphate, respectively [42, 58, 59, 60]. The pH of cow dung and sheep manure vermicompost came out to be 8.48 and 8.6 [60], cattle manure had a pH of 6.0–6.7 [61, 62], pig manure had a pH of 5.3–5.7 [63, 64] and the one derived from sewage sludge had a pH of 7.2 [65]. The lower pH of the final vermicomposts might be due to production of CO2 and organic acids by microbes during the process of bioconversion of different substrates in the feed given to earthworms [66, 67]. The decline in pH might be due to reduction in quantity of different types of volatile solids and to the growth of earthworm’s biomass. The larger the increase in biomass growth, there was greater the reduction in volatile solids and hence the more shift toward the acidic condition [68, 69]. A decrease in pH might be an key factor in nitrogen retention as this element is lost as volatile ammonia at higher pH value. The lower pH was due to production of fulvic acid and humic acid during decomposition [70].

The change of mesophilic to thermophilic condition changes pH from acidic to alkaline due to conversion of organic –N- to NH4+ [71, 72, 73, 74]. Rynk et al. [75] suggested that the excess of organic nitrogen not required by microbes was released as ammonia which got dissolved in water and increased the pH of the vermicompost. Datar et al. [76], Singh et al. [77], Goswami et al. [78], Huang et al. [79] and Lleo et al. [80] also reported an increase in pH during vermicomposting of solid waste, beverage biosludge, tea factory coal ash, fruit & vegetable waste and home waste respectively. They asserted that an increase in pH during composting and vermicomposting process was due to progressive utilization of organic acids and an increase in mineral constituents of the waste. On the other hand Song et al. [67] and Ravindran et al. [81] observed decrease in pH during vermicomposting of fermented tannery waste and animal manure spiked with mushroom residue respectively. They attributed that production of CO2, organic acids and joint action of earthworms and microbes lead to low pH of the vermicompost.

Electric conductivity (EC) is a good indicator of the suitability and safety of vermicompost [82]. The reports regarding electrical conductivity during vermicomposting process are contradictory, some workers reported decrease in electrical conductivity [77, 83, 84, 85] and others an increase in electrical conductivity [67, 69, 86, 87]. The decrease in pH might be due to decrease in ions after forming a complex, whereas the increase in pH might be due to the degradation of organic matter to release various types of cations of different mineral salts in available forms such as phosphate, ammonium and potassium [88, 89] or may be due to loss of organic matter [16].

5.2. Nitrogen

Earthworms may influence microbial N transformation such as mineralization, nitrification and denitrification through their interaction with soil biota and increase concentration of ammonia in the fresh vermicasts [90]. Nitrogen generally declines during aerobic composting due to use of nitrogen by the rapidly multiplying heterotrophic bacteria but it increases during vermicomposting [69, 77, 91]. Chaudhuri et al. [66] reported the decrease in potassium and nitrogen content during the vermicomposting of kitchen waste with the help of P. excavatus. This might be due to NH3 volatilization, incorporation into earthworm tissue and leaching into bedding material with as well as without earthworms or due to release of ammonia [92]. Although, nitrogen content increased during the process of vermicomposting of various materials [93, 94, 95, 96, 97] but final TKN content in vermicompost was always dependent on the initial nitrogen present in the feed material and the degree of decomposition [98, 99, 100]. Decrease in pH may also have an important effect in nitrogen retention as nitrogen is lost as volatile ammonia at high pH [89]. There is also might be good relation between nitrogen and C/N ration of the initial feed mixture because less the C/N ratio the greater will be the decomposition rate of the organic waste and hence the greater the increase in nitrogen [101]. Casting and burrowing behavior of earthworms increase C and N mineralization due to nitrogen fixing bacteria [102]. According to Needham [103], Tillinghast [104] and Viel et al. [105] loss in organic carbon may be the critical factor for nitrogen addition. Mucoproteins in the mucus secreted by epidermal glands, urea excreted through nephridia and ammonia through the gut with cast materials also helped in enhancing the nitrogen content in the vermicompost. Dead worms and their decaying tissues also adds a significant amount of nitrogen to the vermicomposting system.

Whalen et al. [106] found that microbial biomass was responsible for maximum of nitrogen released from decomposing earthworm tissue. Whalen et al. [107] observed that juvenile of L. terrestrisexcreted significantly more nitrogen as compare to adults at 10°C but in Aporrectodea tuberculatanitrogen excretion was significantly greater for adults as compare to pre-clitellate individual at 18°C. There is a high concentration of NH4-N, NO3-N in soils incubated with earthworms than soil incubated without earthworms for 48 h. Amador et al. [108] reported that organic nitrogen released by dead earthworms reached to 21.1–38.6 t/h/year. Kumar et al. [109] revealed that the content of nitrogen was decreased during vermicomposting which may be due to denitrification, NH3 volatilization and ammonification. The decrease in content of nitrogen was also supported by Benitez et al. [110] with 36% loss of total nitrogen during vermicomposting of sewage sludge.

5.3. Organic carbon and C:N ratio

The C:N ratio is one of the most common indicator used for estimating compost maturity [111]. A decline in C: N ratio <20 indicates an advanced degree of organic matter stabilization and reflects a satisfactory degree of maturity of organic wastes but a C:N ratio ≤15 is preferred for agronomic application [10, 112]. According to Song et al. [67], C:N ration <12 indicated that vermicompost had the preferable properties for field application. Speratti and Whalen [113] observed that mean N2O and CO2 fluxes during the study period tended to be greater from enclosures with added earthworms than the control (no earthworms added), but were non-significantly different due to the low survival rate of introduced earthworms. Better control of earthworm populations in the field is required to fully assess the impact of earthworms on CO2 and N2O fluxes from temperate agro-ecosystems. Similar results was also reported by Tognetti et al. [114] and observed that the rate of CO2 production from vermicompost was much higher as compare to traditional compost. Cabrera et al. [115] reported faster decline in C:N ratio during vermicomposting as compared to compost without earthworm.. However, Atiyeh et al. [116] reported that the C:N ratio of the manure with or without earthworms decreased progressively.

The loss of organic carbon may be mainly due to high CO2 emission via strengthened carbon mineralization due to respiratory activity of earthworms and microorganism [117] which cause faster reduction in carbon and lowering of C:N ratio during vermicomposting. The total organic carbon reduction ranged from 10 to 45% during vermicomposting of organic waste [118] while Singh et al. [82] observed increased in organic carbon content from soil to vermicast. The C:N ratio of vermicompost reduced to 12–17:1 from 21–69:1 [11, 16, 55, 119, 120]. Saha et al. [121] and Pramanik [122] observed that decrease in C:N ratio attributed to an increase in earthworm abundance which leads to rapid decrease in organic carbon due to enhancement in organic matter oxidation. Aira and Dominguez [123] reported that rise in microbial biomass during vermicomposting increase carbon losses. Briones et al. [124] suggested that calciferous organs of worms provided a mechanism of CO2 regulation and both environmental and metabolic CO2 could be fixed by this organ.

5.4. Phosphorus

Phosphorus is an important nutrient for growth of plants and is used for protein formation, metabolism, photosynthesis, seed germination and flower and fruit formation [125]. However, phosphorus in soil is in mineral form which was readily available for plants but the potential activity of earthworm and phosphate solublising microorganisms increases phosphorus availability for plants [120, 126].

Gomez et al. [85]; Pramanik [122]; Lim et al. [127]; Singh et al. [128]; Hanc and Chadimova [129] asserted that the rise in total Phosphorus during vermicomposting was probably due to mineralization and mobilization of phosphorus as a result of bacterial and fecal phosphatase activity of earthworms. When organic matter passed through the earthworm gut, some amount of phosphorus is converted into more available form due to enzyme phosphatase and further release of might be attributed to the phosphorus solublizing microorganisms present in the cast [20]. In 1999, Patron et al. [130] noted that earthworm activity accelerated transformation of organic Phosphorus to plant available phosphorus form. Lim et al. [127] and Bayon and Binet [131] observed an increase of phosphorus by 25% and 2.4–49.5% by employing E. fetida and E. eugeniaefor vermicomposting of paper waste sludge and rice husk respectively. Ghosh et al. [132] observed that vermicomposting of different waste materials resulted into 12–20.9% increase in easily extractable phosphorus.

According to Kaviraj and Sharma [16], organic matter decomposition by microbes resulted into acid production which is the major mechanism for solubilization of insoluble phosphorus and potassium. Therefore, presence of a huge number of gut microbes in earthworm might play an important role in increasing phosphorus content in the vermicompost. Mba [133] and Wan and Wong [134] studied the effects of Bacillus megaterium(phosphate solubilizing bacteria) and earthworm E. fetidaand Pheretima guillelmion Phosphorus turn over and transformation in soil. They found that the number of B. megateriumwas increased in all the treatments with earthworm. The activity of acid phosphatase increased in the treatments having earthworm Pheretima guillelmialong with a significant increase in both inorganic and water soluble phosphorus. Acid phosphatase promoted the hydrolysis rate of organic phosphorus into inorganic phosphorus and the B. megateriumfound in the worm casts of E. euginae. According to Pramanik [122], the higher phosphorus content might pertain to the higher adsorption rate of NO3 anions and replacement of PO4 ions from humic colloids. Hanc and Chandimova [129] resulted that total phosphorus content in the final vermicompost was 11% higher than control. They also observed that enhanced number of microflora in the earthworm gut might have played an important role in release of available phosphorus.

5.5. Potassium

There are contradictory reports regarding the total potassium content in vermicomposts obtained from different substrates due to the differences in the chemical nature of the initial raw materials [135]. Song et al., [67], Gomez et al. [85], Benitez et al. [110], Lim et al. [127] and Bhat et al. [136] have reported higher potassium concentrations during vermicomposting process. Increase in potassium content in vermicompost suggested that earthworms have symbiotic gut microflora with secreted mucus and water to degrade the ingested substrate which cause release of easily assailable metabolites [89]. Garg et al. [83], Singh et al. [128] and Elvira et al. [137] reported that total potassium concentration decrease in vermicompost. This decrease in concentration of potassium may attributed to the variation in chemical composition of initial feed mixture or due to leaching of potassium because of low water holding capacity of the vermicompost [128].

Guerra-Rodriguez et al. [72], Delgado et al. [138] and Suthar [139] revealed that mineralization process significantly enhanced the concentration of exchangeable potassium during vermicomposting. Suthar [20] and Nahrul Hayawin et al. [96] also reported higher potassium content in the vermicompost produced from distillery sludge, oil farm waste and food industrial sludge respectively. Lim et al. [127] observed 15–121.4% increase in potassium content by using E. eugeniaefor vermicomposting of rice husk. Huang et al. [84] cultured hatchlings, juveniles and adults E. fetidainto three different culture tubs to study the changes in bacterial and fungal community composition. They observed that potassium concentration in vermicompost cultured with juveniles was more as compare to vermicompost cultured with adults earthworms. Vermicompost cultured with juveniles has 33.3% more potassium as compare to vermicompost cultured with adults.

5.6. Bioaccumulation of heavy metals and its effect on earthworms

The increasing exploitation of natural resources by human beings during the past few centuries has adversely affected the global balance of heavy metals causing a gradual increase in the concentration of metals in the soil ecosystem [140]. In order to maintain the environmentally sound soil quality, investigators are seeking methods to reduce the mobility of heavy metals from wastes to soil ecosystem. Metal mobility and availability can be reduced by raising the soil pH [141]. Phyto-remediation is known as the most viable and environment friendly technology. But, a limited number of plants have been found to have phyto-accumulation ability and a very less number can be used for field phyto-remediation because of low biomass production. Therefore, earthworms appears to be a valuable substitute for control of metals in contaminated soils [142]. According to Hopkin [143], the earthworms have capacity to control metals, particularly trace metals, such as Cu and Zn, in their bodies. Earthworms can also be used as bioindicators for assessing the level of soil contamination with agricultural runoff, heavy metals, acid rain, pesticides etc. [144].

The capability of earthworms to mitigate the heavy metal toxicity and to increase the nutrient profile of organic wastes might be useful in sustainable land restoration practices [20]. Heavy metals have the capability to bind with ligands of the tissues and thus leads to their bioaccumulation in the food chain [145]. A positive correlation between metal concentrations in the earthworms and those in the soils were observed with differences in bioaccumulation factors for different metals, this could be due to a variable metabolic requirement of earthworms for metals [146]. The effects of sub lethal concentrations of lead nitrate on reproduction and growth of P. excavatuswas studied by Maboeta et al. [147]. The growth was affected negatively by the presence of lead while maturation rate and cocoon production was not affected.

Earthworms are have the capability to inhabit and survive in sites contaminated with metals [148] and have the ability to accumulate heavy metals in the cells of yellow tissue [149]. Earthworm populations may develop mechanism by which they can tolerate or resist the effect of metal induced stress. Such tolerance or resistance acquired by earthworms either through a variation in their genetic structure or reversible changes in an earthworm’s physiology. Toxicity tests done by various authors have shown that heavy metal pollution negatively affects life-history of earthworms such as growth, reproduction and survival [150]. Beyer et al. [151] studied the bioaccumulation of methyl-mercury in the E. fetidaand its effect on regeneration after excision of the caudal end. They found that earthworms treated with 25 ppm or more methyl-mercury did not survive while the survival rates in other concentrations were 97% in control, 92% in 1 ppm and 79% in 5 ppm after 12 weeks. All surviving earthworms in the control regenerated but 29% of earthworms in 5 ppm group only healed without regenerating their tail end. An opposite results were reported by Boudou and Ribeyre [152] and Burton et al. [153] that the absolute concentration of total mercury and monomethyl-mercury bioaccumulated in E. fetidawere higher in the earthworm exposed to the higher mercury soils and lower in the less mercury contaminated soils. The bioaccumulation factors for total mercury and monomethyl-mercury were larger in earthworms exposed to less contaminated soils and smaller in more mercury contaminated soils. Zhang et al. [154] reported that Bioaccumulation factors of methyl-mercury from soil to earthworms were much higher than those of total mercury, which suggested that methyl-mercury might be more easily absorbed by and accumulated in earthworms because of its lipid solubility.

Maenpaa et al. [155] showed that the treatment of high Phosphorus significantly reduced lead, zinc and cadmium bioavailability to the earthworm which was due to formation of metal–phosphate complex in the soils. This amendment reduce ecological risk to soil-inhabiting invertebrates exposed to heavy metal contaminated soils. Malley et al. [156] reported that earthworm act as an indicator for heavy metals toxicity that are present in the materials and are bioconverted, giving an indication of potential environmental hazard. The capacity of earthworm to uptake and redistribute heavy metals in their body lead to a balance between uptake and excretion which helps them to survive in metal contaminated soil. Kızılkaya [157] observed that the earthworm L. terrestrishad the capacity to accumulate significant levels of zinc, and thus earthworm ingestion may result in zinc transfer to higher trophic levels. He observed that the cast and earthworm bodies receiving the highest Zn dose showed significantly higher Zn content than the non-treated soil. The effect of earthworm (L. mauritii) activity on mobility of Pb2+ and Zn2+ in the soil (DTPA-extractable) and its composting potential in the presence of these metals was investigated by Maity et al. [158] and suggested that the use of L. mauritiiin amelioration of metal contaminated soil. Liua et al. [159] noticed that earthworm treatment increased the biomass of cabbage and decreased the bioaccumulation of Cd and Cu in the cabbage plants.

Udovic and Lestan [160] reported that bioavailability of Pb and Zn before and after soil leaching with EDTA with two earthworm species, L. rubellusand E. fetida,actively regulated soil pH, but did not significantly change Pb and Zn fractionation in remediated soil. Sivakumar and Subbhuraam [161] reported the effects of Cr (III) and Cr (VI) on the survival, behavior, and morphology of the earthworm, E. fetida, in water at pH 6, 7 and 8 and their toxicity in 10 different soils and an organic substrate. A decrease in the pH of water resulted in increased toxicity of Cr to the earthworm. In water, both Cr species produced behavioral changes and morphological symptoms. Wei-bao and Hong-qiang [162] elucidated role of earthworms and microbes in improving soil structure and controlling bioavailability of soil nutrients including heavy metals through bio-absorption, enrichment, precipitation, dissolution, and oxidation–reduction. The influence of salinity on partitioning of, uptake in and toxicity of zinc to earthworms was studied by Owojori et al. [163] by exposing E. fetidain the laboratory for 28 days in OECD artificial soil spiked with either NaCl (experiment 1) or a combination of Zn and NaCl (experiment 2) and observed that the cocoon production was significantly affected by increased NaCl and Zn administered as individual substances, and the effects were more severe when both substances were present together. It was concluded that an increase in salinity had an additive to synergistic effect on influencing the toxicity of Zn to these earthworms. Frank et al. [164] noted slight increase in the metal contents in worm castings except for Cr and Zn over the worm feed mixture. This could be explained by the fact that organic matter was being reduced on passage through the gut of worms but actually worms did not appear to bioaccumulate metals within their tissue. Singh et al. [77], Kaur et al. [91] and Deolalikar et al. [165] reported an increase in heavy metal content in the vermicompost of paper mill sludge. The increase was more appreciable for Fe and Cu. The weight and volume reduction due to breakdown of organic matter during vermicomposting might have been the reason for increase in heavy metal concentrations in vermicompost. A 2% increase in Cu and a decline in the concentration of Mn, Zn and Pb in vermicompost were reported by Khwairakpam and Bhargava [166].

Song et al. [67] conducted a pilot scale trial to investigate the response of heavy metals and nutrients changes to composting animal manure spiked with mushroom residues with and without earthworms. They resulted that composting without earthworm have high concentration of heavy metals, that is, As, Pb, Cu, Zn, while that in vermicompost concentration of heavy metals decreased significantly relative to the compost. The decrease of metals concentrations in the vermicompost occurred for at least two reasons. First, vermicompost processed by earthworms had high level of humic acid which posed a stronger sorption effect on formation of stable metal humus complex especially for Cu and Zn [167]. Second, bioaccumulation of heavy metals by earthworms tissues with the help of epithelial layer and body fluids [168]. Singh et al. [82] and Kharrazi et al. [120] also observed decrease in concentration of heavy metals in the final vermicompost material. Soobhany et al [19] concluded that the reduction in toxic heavy metals by inoculating earthworm in the organic waste might be helpful in gaining clean environment.

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6. Conclusion

Growth rate of earthworm, clitellum development, cocoon production and population buildup of earthworm were depend upon the physico-chemical composition of the feeding materials, types of feed mixture and environmental conditions like temperature, moisture and pH determine the sexual maturation in earthworms. Out of the various species of earthworms, Eisenia fetidais the most preferred species as it is hardy, prolific breeder and accepts a wide variety of food. Thus vermicast egested by the earthworm is a good source of N and P which is easily available to the plants and it has many advantages as compared to fertilizer and compost. The bioaccumulation of heavy metals by earthworms may be helpful to reduce the metal from organic waste. The feeding and casting activity of earthworm can stabilized the soil structure and change its physico-chemical properties and thus played an important role in sustainable agriculture.

References

  1. 1. Darwin C. The Formation of Vegetable Mould through the Action of Worms, with Observation on their Habits. London: Murray; 1881. 326 pp
  2. 2. Fisher C. The nutritional value of earthworm meal for poultry. In: Earthworm in Waste and in Environment. Vol. 1988. The Hague, The Netherlands: SPB Academic Publishing; pp. 181-192
  3. 3. Frederickson J, Butt KR, Morris RM, Daniel C. Combining vermiculture with traditional green waste composting system. Soil Biology and Biochemistry. 1997;29:725-730
  4. 4. Edwards CA. Breakdown of animal, vegetable and industrial organic wastes by earthworm. Agriculture, Ecosystems and Environment. 1988;24:21-31
  5. 5. Singh J, Kaur A. Vermicompost as a strong buffer and natural adsorbent for reducing transition metals, BOD, COD from industrial effluent. Ecological Engineering. 2014;74:13-19
  6. 6. Julka JM. General morphology and characters of taxonomic importance. Earthworm Resources and Vermiculture. 1993:17-26
  7. 7. Singh S, Singh J, Vig AP. Effect of abiotic factors on the distribution of earthworms in different land use patterns. The Journal of Basic & Applied Zoology. 2016;74:41-50
  8. 8. Dominguez J, Aira M. Twenty years of the earthworm biotechnology research program at the university of Vigo, Spain. IJESER. 2012;3(2):1-7
  9. 9. Bhattacharjee G, Chaudhuri PS. Cocoon production, morphology, hatching pattern and fecundity in seven tropical earthworms species-a laboratory-based investigation. Journal of Biosciences. 2002;27:283-294
  10. 10. Padmavathiamma PK, Loretta YL, Kumari UR. An experimental study of vermi-biowaste composting for agricultural soil improvement. Bioresource Technology. 2008;99:1672-1681
  11. 11. Tripathi G, Bhardwaj P. Comparative studies on biomass production, life cycles and composting efficiency ofEisenia fetida(Savigny) andLampito mauritii(Kinberg). Bioresource Technology. 2004;92:275-278
  12. 12. Reinecke AJ, Viljoen SA, Saayman RJ. The suitability ofEudrilus eugeniae,Perionyx excavatusandEisenia fetida(Oligochaeta) for vermicomposting in southern Africa in terms of their temperature requirements. Soil Biology and Biochemistry. 1992;24:1295-1307
  13. 13. Dash MC, Senapati BK. Vermitechnology: Potentiality of Indian earthworms for vermicomposting and vermifeed. In: Mishra MM, Kapoor KK, editors. Proc. Soil Biol. Symp. Hisar: HAU Press; 1985. pp. 61-69
  14. 14. Shinde PH, Naik RL, Narizkar RB, Kadam SK, Khair VM. Evaluation of vermicompost. In: Proceedings of the National Seminar on Organic Farming. Pune: Mahatma Phule Krishi Vidyapeeth; 1992. pp. 54-55
  15. 15. Singh J. Habitat preferences of selected Indian earthworm species and their efficiency in reduction of organic materials. Soil Biology and Biochemistry. 1997;29:585-588
  16. 16. Kaviraj, Sharma S. Municipal solid waste management through vermicomposting employing exotic and local species of earthworms. Bioresource Technology. 2003;90:169-173
  17. 17. Dominguez J, Edwards CA, Ashby J. The biology and population dynamics ofEudrilus eugeniae(Kinberg) (Oligochaeta) in cattle waste solids. Pedobiologia. 2001;45:341-353
  18. 18. Bhattacharya SS, Chattopadhyay GN. Increasing bioavailability of phosphorus from fly ash through vermicomposting. Journal of Environmental Quality. 2002;31:2116-2119
  19. 19. Soobhany N, Mohee R, Garg VK. Comparative assessment of heavy metals content during the composting and vermicomposting of municipal solid waste employingEudrilius eugeniae. Waste Management. 2015;39:130-145
  20. 20. Suthar S. Bioremediation of aerobically treated distillery sludge mixed with cow dung by using an epigeic earthwormEisenia fetida. Environmentalist. 2008;28:76-84
  21. 21. Suthar S, Singh S. Comparison of some novel polyculture and traditional monoculture vermicomposting reactors to decompose organic wastes. Ecological Engineering. 2008;33:210-219
  22. 22. Elvira C, Dominguez J, Mato S. The growth and reproduction ofLumbricus rubellusandDendrobaena rubidain cow manure mixed cultures withEisenia andrei. Applied Soil Ecology. 1996;5:97-103
  23. 23. Reinecke AJ, Kried JR. Influences of temperature on the reproduction of the earthwormEisenia fetida. South African Journal of Zoology. 1981;16:96-100
  24. 24. Reinecke AJ, Venter JM. Moisture preference, growth and reproduction of the compost wormEisenia fetida(Oligochaeta). Biology and Fertility of Soils. 1987;3:135-141
  25. 25. Edwards CA, Norman QA, Sherman R. Vermiculture Technology, Earthworms, Organic Waste and Environmental Management. CRC Press; 2011. pp. 17-19
  26. 26. Dominguez J, Edwards CA. Effects of stocking rate and moisture contents on the growth and maturation ofEisenia anderi(Oligochaeta) in pig manure. Soil Biology & Biochemistry. 1997;29:143-746
  27. 27. Lalander CH, Komakech AJ, Vinneras B. Vermicomposting as manure management strategy for urban small-holder animal farms- Kampala case study. Waste Management. 2015;39:96-103
  28. 28. Edwards CA, Dominguez J, Neuhauser EF. Growth and reproduction ofPerionyx excavatus(Perrier) (Megascolecidae) as factors in organic waste management. Biology and Fertility of Soils. 1998;27:155-161
  29. 29. Edwards CA. The use of earthworms in the breakdown and management of organic waste. In: Edward CA, editor. Earthworm Ecology. Boca Raton: Lewis; 1998. pp. 327-354
  30. 30. Edwards CA, Arancon NQ. Earthworm ecology. In: Edward CA, editor. Boca Raton, Florida: CRC Press; 2004. pp. 345-380
  31. 31. Flack FM, Hartenstein R. Growth of the earthwormEisenia fetidaon microorganisms and cellulose. Soil Biology and Biochemistry. 1984;16:491-495
  32. 32. Bostrom U. Growth of earthwormsAllolobophora caliginosain soil mixed with barley, Lucerne or meadow fescue at various stages of decomposition. Pedobiologia. 1987;30:311-321
  33. 33. Shipitalo MJ, Protz R, Tomlin AD. Effect of diet on the feeding and casting activity ofLumbricus terrestrisandLumbricus rubellusin laboratory culture. Soil Biology & Biochemistry. 1988;20:233-237
  34. 34. Watanabe H, Tsukamoto J. Seasonal changes in size class and stage structure of the lumbricidEisenia fetidapopulation in a field compost and its practical application as the decomposer of organic waste matter. Revue d'Écologie et de Biologie du Sol. 1976;13:141-146
  35. 35. Viljoen SA, Reinecke AJ. The life cycle of African night crawler, (Eudrilus eugeniae Oligochaeta). South African Journal of Zoology. 1989;24:27-32
  36. 36. Dominguez J, Edwards CA, Webster M. Vermicomposting of sewage sludge: Effect of bulking materials on the growth and reproduction of the earthwormEisenia andrei. Pedobiologia. 2000;44:24-32
  37. 37. Suthar S. Vermicomposting potential ofPerionyx sansibaricus(Perrier) in different waste materials. Bioresource Technology. 2007;98:1231-1237
  38. 38. Hartenstein R, Neuhauser EF, Kaplan DL. Reproductive potential of the earthwormEisenia fetida. Oecologia. 1979;43:329-340
  39. 39. Neuhauser EF, Hartenstein R, Kaplan DL. Growth of the earthwormEisenia fetidain relation to population density and food rationing. Oikos. 1980;35:93-98
  40. 40. Neuhauser EF, Loehr RC, Malecki MR. The potential of earthworms for managing sewage sludge. In: Edwards CA, Neuhauser EF, editors. Earthworms in Waste and Environmental Management. The Hague: SPB Academic Publishing; 1988. pp. 9-20
  41. 41. Gunadi B, Susanto SJA, Sosrodjojo PS Laboratory and large scale indoor condition for culturing two species of redworms (Eisenia anderi and Eisenia fetida) using tea leaf as a food. Proceeding of the 6th International Symposium on Earthworm Ecology. Vigo, Spain, August 30–September 4; 1998
  42. 42. Gunadi B, Edwards CA. The effect of multiple application of different organic wastes on the growth, fecundity and survival ofEisenia fetida. Pedobiologia. 2003;47:321-330
  43. 43. Bouwman H, Reinecke AJ. A defined medium for the study of growth and reproduction of the earthwormEisenia fetida(Oligochaeta). Biology and Fertility of Soils. 1991;10:285-289
  44. 44. Reinecke AJ, Viljoen SA. Reproduction of the African earthworm,Eudrilus eugeniae(Oligochaeta) – Cocoons. Biology and Fertility of Soils. 1988;7:23-27
  45. 45. Evans AC, Guild WJM. Studies on the relationship between earthworms and soil fertility. On the life cycles of some British Lumbricidae. Annals of Applied Biology. 1948;35:471-483
  46. 46. Venter JM, Reinecke AJ. The life cycle of the compost wormEisenia fetida(oligochaeta). South African Journal of Zoology. 1988;23:161-165
  47. 47. Satchell JE. Lumbricidae. In: Burges A, Raw, editors. Soil Biology. London: Academic Press; 1967. pp. 259-322
  48. 48. Olive PJW, Clark RB. Physiology of reproduction. In: Mill PJ, editor. Physiology of Annelids. London: Academic Press; 1978. pp. 271-368
  49. 49. Lavelle P. Strategies de reproduction chez les vers de terre. Acta Oecologia. 1981;21:17-133
  50. 50. Senapati BK, Sahu SK. Reproductive biology (cocoon morphology, life cycle pattern and life table analysis) in earthworms. In: Earthworm Resources and Vermiculture (Calcuuta: Zoological Survey of India). 1993. pp. 79-96
  51. 51. Lee KE. Earthworm: Their Ecology and Relationship with Soil and Land Use. Sydney: Academic Press; 1985. p. 411
  52. 52. Edwards CA, Bohlen PJ. Biology and Ecology of Earthworm. 3rd ed. New York, London: Chapman and Hall; 1996
  53. 53. Barne AZ, Striganova BR. Evaluation of production parameters of earthwormsEiseniella tetraederain laboratory culture. Biology Bulletin. 2005;32:323-326
  54. 54. Jager T, Reinecke SA, Reinecke AJ. Using process based modeling to analysed earthworm life cycles. Soil Biology & Biochemistry. 2006;38:1-6
  55. 55. Gunadi B, Blount C, Edwards CA. The growth and fecundity ofEisenia fetida(Savigny) in cattle solids pre-composted for different periods. Pedobiologia. 2002;46:15-23
  56. 56. Kemppainnen E. Nutrient content and fertilizer value of live stick manure with special reference to cow manure. Annales Agriculturae Fenniae. 1989;28:163-284
  57. 57. Kaplan DL, Hartenstein R, Neuhauser EF, Malecki MR. Physico-chemical requirements in the environment of the earthwormEisenia fetida. Soil Biology & Biochemistry. 1980;12:347-352
  58. 58. Loh TC, Lee YC, Liang JB, Tan D. Vermicomposting of cattle and goat manues byEisenia fetidaand their growth and reproduction performance. Bioresource Technology. 2005;96:111-114
  59. 59. Li X, Xing M, Yang J, Huang Z. Compositional and functional features of humic acid-like fractions from vermicomposting of sewage sludge and cow dung. Journal of Hazardous Materials. 2011;185:740-748
  60. 60. Gutierrez-Miceli FA, Santiago-Borraz J, Molina JAM, Nafate CC, Abud-Archila M, Llaven MAO, Rincon-Rosales R, Dendooven L. Vermicompost as a soil supplement to improve growth, yield and fruit quality of tomato (Lycopersicum esculentum). Bioresource Technology. 2007;98:2781-2786
  61. 61. Alves FL, Cadore S, Jardim WF, Arruda MAZ. River sediment analysis by slurry sampling FAAS: Determination of copper, zinc and lead. Journal of the Brazilian Chemical Society. 2001;12:799-803
  62. 62. Jordao CP, Pereira MG, Einloft R, Santana M B, Bellato CR, de Mello JWV. Removal of cu, Cr, Ni, and Zn from electroplating wastes and synthetic solutions by vermicompost of cattle manure. Journal of Environmental Science and Health . 2002;37:875-892
  63. 63. Atiyeh RM, Edwards CA, Subler S, Metzger JD. Pig manure vermicompost as a component of a horticultural bedding plant medium: effects on physicochemical properties and plant growth. Bioresource Technology. 2001;78:11-20
  64. 64. Atiyeh RM, Arancon NQ, Edwards CA, Metzger JD. The influence of earthworm-processed pig manure on the growth and productivity of marigolds. Bioresource Technology. 2002;81:103-108
  65. 65. Masciandaro G, Ceccanti B, Garcia C. “In situ” vermicomposting of biological sludges and impacts on soil quality. Soil Biology & Biochemistry. 2000;32:1015-1024
  66. 66. Chaudhuri PS, Pal TK, Bhattacharjee G, Dey SK. Chemical changes during vermicomposting (Perionyx excavatus) of kitchen waste. Tropical Ecology. 2000;41:107-110
  67. 67. Song X, Liu M, Wu D, Qi L, Ye C, Jiao J. Heavy metals and nutrient changes during vermicomposting animal manure spiked with mushroom residues. Waste Management. 2014;34:1977-1983
  68. 68. Ndegwa PM, Thompson SA, Das KC. Effects of stocking density and feeding rate on vermicomposting of biosolids. Bioresource Technology. 2000;71:5-12
  69. 69. Yang J, Lv B, Zhang J, Xing M. Insight into the roles of earthworm in vermicomposting of sewage sludge by determining the water extracts through chemical and spectroscopic methods. Bioresource Technology. 2014;154:94-100
  70. 70. Albanell E, Plaixats J, Cabrero T. Chemical change during vermicomposting (Eisenia foetida) of sheep manure mixed with cotton industrial waste. Biology and Fertility of Soils. 1988;6:266-269
  71. 71. Fang M, Wong JWC, Li GX, Wong MH. Changes in biological parameters during co-composting of sewage sludge and coal ash residue. Bioresource Technology. 1998;64:55-61
  72. 72. Guerra-Rodriguez E, Vazquez M, Diaz-Ravina M. Cocomposting of barley wastes and solid poultry manure. Bioresource Technology. 2000;75:223-225
  73. 73. Guerra-Rodriguez E, Diaz-Ravina M, Vazquez M. Cocomposting of chestnut burr and leaf litter with solid poultry manure. Bioresource Technology. 2001;78:107-109
  74. 74. Beck-friis B, Smars S, Jonsson H, Kirchmann H. Gaseous emission of carbon dioxide, ammonia and nitrous oxide from organic household waste in a compost reactor under different temperature regimes. Journal of Agriculture Engineering Research. 2001;78:423-430
  75. 75. Rynk RM, Kamp VD, Willson GG, Singley ME, Richard TL, Kolega JJ, Gouin FR, Laliberty L Jr, Kay D, Murphy DH, Hoitink AJ, Brinton WF. On-farm composting handbook. In: Rynk R, editor. New York, US: Northeast Regional Agricultural Engineering Service, Cooperative Extension; 1994. 186 p
  76. 76. Datar MT, Rao MN, Reddy S. Vermicomposting-a technology option for solid waste management. Journal of Solid Waste Technology Management. 1997;24:89-93
  77. 77. Singh J, Kaur A, Vig AP, Rup PJ. Role ofEisenia fetidain rapid recycling of nutrients from bio sludge of beverage industry. Ecotoxicology and Environmental Safety. 2010;73:430-435
  78. 78. Goswami L, Sarkar S, Mukherjee S, Das S, Barman S, Raul P, Bhattacharyya P, Mandal NC, Bhattacharya S, Bhattacharya SS. Vermicomposting of tea factory coal ash: Metal accumulation and metallothionein response inEisenia fetida(Savigny) andLampito mauritti(Kinberg). Bioresource Technology. 2014;166:96-102
  79. 79. Huang K, Fusheng L, Wei Y, Fu X, Chen X. Effects of earthworm on physicochemical properties and microbial profiles during vermicomposting of fresh fruit and vegetables wastes. Bioresource Technology. 2014;170:45-52
  80. 80. Lleo T, Albacete E, barren R, Xavier F, Adriana A, Sanchez A. Home and vermicomposting as sustainable options for biowaste management. Journal of Cleaner Production. 2013;47:70-76
  81. 81. Ravindran B, Contreras-Ramos SM, Sekaran G. Changes in earthworm gut associated enzymes and microbial diversity on the treatment of fermented tannery waste using epigeic earthwormEudrilus eugeniae. Ecological Engineering. 2015;74:394-401
  82. 82. Singh SP, Singh J, Vig AP. Earthworm as ecological engineers to change the physico-chemical properties of soil: Soil vs vermiast. Ecological Engineering. 2016;90:1-5
  83. 83. Garg VK, Yadav YK, Sheoran A, Chand S, Kaushik P. Livestock excreta management through vermicomposting using an epigeic earthwormEisenia fetida. Environmentalist. 2006;26:269-276
  84. 84. Huang K, Fusheng L, Wei Y, Chen X, Fu X. Changes of bacterial and fungal community compositions during vermicomposting of vegetable wastes byEisenia fetida. Bioresource Technology. 2013;150:235-241
  85. 85. Gomez MJF, Nogales R, Plante A, Plaza C, Fernandez JM. Application of a set of complementary techniques to be understand how varying the proportion of two wastes affects humic acid produced by vermicomposting. Waste Management. 2015;35:81-88
  86. 86. Sellami FR, Jarboui S, Hachicha K, Medhioub EA. Co-composting of oil exhausted olive-cake, poultry manure and industrial residues of agro-food activity for soil amendment. Bioresource Technology. 2008;99:1177-1188
  87. 87. Hait S, Tare V. Vermistabilization of primary sewage sludge. Bioresource Technology. 2011;102:2812-2820
  88. 88. Lazcano C, Gomez-Brandon M, Dominguez J. Comparison of the effectiveness of composting and vermicomposting for the biological stabilization of cattle manure. Chemosphere. 2008;72:1013-1019
  89. 89. Khwairakpam M, Bhargava R. Vermitechnology for sewage sludge recycling. Journal of Hazardous Materials. 2009;161:948-954
  90. 90. Blair JM, Parmelee RW, Lavelle P. Influences of earthworms on biogeochemistry. In: Hendrix PF, editor. Earthworm Ecology and Biogeography in North America. Boca Raton: Lewis; 1995. pp. 127-158
  91. 91. Kaur A, Singh J, Vig AP, Dhaliwal SS, Rup PJ. Cocomposting with and withoutEisenia fetidafor conversion of toxic paper mill sludge into soil conditioner. Bioresource Technology. 2010;101:8192-8198
  92. 92. Guest CA, Johnston CT, King JJ, Allenman JJ, Tishmack JK, Norton LD. Chemical characterisation of synthetic soil from composting coal combustion and pharmaceutical by- products. Journal of Environmental Quality. 2001;80:246-253
  93. 93. Manna MC, Jha S, Ghosh PK, Acharya CL. Comparative efficacy of three epigeic earthworms under different deciduous forest litters decomposition. Bioresource Technology. 2003;88:197-206
  94. 94. Jadia CD, Fulekar MH. Vermicomposting of vegetable waste: A biophysico-chemical process based on hydro-operating bioreactor. African Journal of Biotechnology. 2008;7:3723-3730
  95. 95. Adi AJ, Noor ZM. Waste recycling: Utilization of coffee grounds and kitchen waste in vermicomposting. Bioresource Technolology. 2009;100:1027-1030
  96. 96. Nahrul Hayawin Z, Abdul Khalil HPS, Jawaid M, Hakimi Ibrahim M, Astimar AA. Exploring chemical analysis of vermicompost of various oil palm fibre wastes. Environmentalist. 2010;30:273-278
  97. 97. Singh J, Kaur A, Vig AP. Bioremediation of distillery sludge into soil-enriching material through vermicomposting with the help ofEisenia fetida. Applied Biochemistry and Biotechnology. 2014;174:1403-1419
  98. 98. Araujo Y, Luizao FJ, Barros E. Effect of earthworm addition on soil nitrogen availability, microbial biomass and litter decomposition in mesocosms. Biology and Fertility of Soils. 2004;39:146-152
  99. 99. Garg VK, Kaushik P. Vermistabilization of textile mill sludge spiked with poultry droppings by an epigeic earthwormEisenia fetida. Bioresource Technology. 2005;96:1063-1071
  100. 100. Hobson AM, Frederickson J, Dise NB. CH4 and N2O from mechanically turned windrow and vermicomposting systems following in–vessel pre-treatment. Waste Management. 2005;25:345-352
  101. 101. Parmanik P, Ghosh GK, Ghosal PK, Banik P. Changes in organic C, N, P and K and enzymatic activities in vermicompost of biodegradable organic wastes under liming and microbial inoculants. Bioresource Technology. 2007;98:2485-2494
  102. 102. Crusmey JM, LeMoine JM, Vogel CS, Nadelhoffer KJ. Historical patternsof exotic earthworm distribution inform contemporary associations with soilphysical and chemical factors across a northern temperate forest. Soil Biology & Biochemistry. 2014;68:503-514
  103. 103. Needham AE. Components of nitrogenous excreta in the earthworms Lumbricus terrestris and Eisenia fetida. The Journal of Experimental Biology. 1957;34:425-446
  104. 104. Tillinghast EK. Excretory pathways of ammonoa and urea in the earthworm Lumbricus terrestris. The Journal of Experimental Zoology. 1967;166:295-300
  105. 105. Viel M, Sayag D, Andre L. Optimization of agricultural, industrial waste management through in-vessel composting. In: de Bertoldi M, editor. Compost: Production, Quality and Use. Elseiver Appl. Sci. Essex; 1987. pp. 230-237
  106. 106. Whalen JK, Paustian KH, Parmelee RW. Simulation of growth and flux of carbon and nitrogen through earthworm. Pedobiologia. 1999;43:537-546
  107. 107. Whalen JK, Parmelee RW, Subler S. Quantification of nitrogen excretion rates for three lumbricid earthworms using 15N. Biology and Fertility of Soils. 2000;32:347-352
  108. 108. Amador JA, Gorres JH, Savin MC. Carbon and nitrogen dynamics inLumbricus terrestrisburrow soil: Relationship to plant residues and macropores. Soil Science Society of America Journal. 2003;67:1755-1762
  109. 109. Kumar R, Verma D, Singh BL, Kumar U, Shweta. Composting of sugar-cane waste by-products through treatment with microorganisms and subsequent vermicomposting. Bioresource Technology. 2010;101:6707-6711
  110. 110. Benitez E, Nogales R, Elvira C, Masciandaro G, Ceccanti B. Enzyme activities as indicators of the stabilization of sewage sludge composting withEisenia fetida. Bioresource Technology. 1999;67:297-303
  111. 111. Suthar S. Recycling of agro-industrial sludge through vermitechnology. Ecological Engineering. 2010;36:1028-1036
  112. 112. Sen B, Chandra TS. Chemolytic and solid state spectroscopic evaluation of organic matter transformation during vermicomposting of sugar industry waste. Bioresource Technology. 2007;98:1680-1689
  113. 113. Speratti AB, Whalen JK. Carbon dioxide and nitrous oxide fluxes from soil as influenced by anecic and endogeic earthworms. Applied Soil Ecology. 2008;38:27-33
  114. 114. Tognetti C, Mazzarino MJ, Laos F. Cocomposting biosolids and municipal organic waste: Effects of process management on stabilization and quality. Biology and Fertility of Soils. 2007;43:387-397
  115. 115. Cabrera ML, Kissel DE, Vigil MF. Nitrogen mineralization from organic residues: Research opportunities. Journal of Environmental Quality. 2005;34:75-79
  116. 116. Atiyeh RM, Arancon NQ, Edwards CA, Metzger JD. Influence of earthworm-processed pig manure on the growth and yield of greenhouse tomatoes. Bioresource Technology. 2000;75:175-180
  117. 117. Deka H, Deka S, Baruah C, Das J, Hoque S, Sarma H, Sarma N. Vermicomposting potentiality of Perionyx excavates for recycling of waste biomass of java citronella-an aromatic oil yielding plant. Bioresource Technology. 2011;102:11212-11217
  118. 118. Garg VK, Gupta R. Optimization of cow dung spiked pre-consumer processing vegetable waste for vermicomposting usingEisenia fetida. Ecotoxicology and Environmental Safety. 2011;74:19-24
  119. 119. Christy MAV, Ramalingam R. Influence of sago wastes-Pressmud mixture on the growth and reproduction of an Indian epigeic earthwormPerionyx excavatus(Perrier). Indian Journal of Environment and Ecoplanning. 2005;10:291-296
  120. 120. Kharrazi SM, Younesi H, Abedini-Torghabeh J. Microbial degradation of waste material for nutrients enrichment and heavy metals removal: An integrated composting-vermicomposting process. International Biodeterionation & Biodegradation. 2014;92:41-48
  121. 121. Saha S, Pradhan K, Sharma S, Alappat BJ. Compost production from municipal solid waste (MSW) employing bioinoculants. International Journal of Environment and Waste Management. 2008;2:572-583
  122. 122. Parmanik P. Changes in microbial properties and nutrient dynamics in bagasse and coir during vermicomposting: Quantification of fungal biomass through ergosterol estimation in vermicomposting. Waste Management. 2010;30:787-791
  123. 123. Aira M, Dominguez J. Optimizing vermicomposting of animal wastes: Effects of rate of manure application on carbon loss and microbial stabilization. Journal of Environmental Management. 2008;88:1525-1529
  124. 124. Briones MJI, Ostle NJ, Piearce TG. Stable isotopes reveal that the calciferous gland of earthworms is a CO2-fixing organ. Soil Biology & Biochemistry. 2008;40:554-557
  125. 125. Hese PR. A Textbook of Soil Chemical Analysis. New York: Chemical Publishing Co., Inc; 1971
  126. 126. Bhat SA, Singh J, Vig AP. Vermistabilization of sugar beet (Beta vulgarisL) waste produced from sugar factory using earthwormEisenia fetida: Genotoxic assessment byAllium cepatest. Environmental Science and Pollution Research. 2015;22:11236-11254
  127. 127. Lim SL, Wu TY, Sim EYS, Lim PN, Clarke C. Biotransformation of husk into organic fertilizer through vermicomposting. Ecological Engineering. 2012;41:60-64
  128. 128. Singh A, Jain A, Sarma BK, Abhilash PC, Singh HB. Solid waste management of temple floral offerings by vermicomposting usingEisenia fetida. Waste Management. 2013;33:1113-1118
  129. 129. Hanc A, Chandimova Z. Nutrient recovery from apple pomace waste by vermicomposting technology. Bioresource Technology. 2014;168:240-244
  130. 130. Patron JC, Sanchez P, Brown GG, Brossard M, Barois I, Gutierrez C. Phosphorus in soil and Brachiaria decumbens plants as affected by the geophagous earthwormPontoscolex corethrurusand phosphorus fertilization. Pedobiologia. 1999;43:547-556
  131. 131. Bayon RC, Binet F. Earthworm changes the distribution and availability of phosphorus in organic substrates. Soil Biology & Biochemistry. 2006;38:235-246
  132. 132. Ghosh M, Chattopadhyay GN, Baral K. Transformation of phosphorus during vermicomposting. Bioresource Technology. 1999;69:149-154
  133. 133. Mba CC. Rock phosphate solubilizing Streptosporangium isolates from casts of tropical earthworms. Soil Biology & Biochemistry. 1997;29:381-385
  134. 134. Wan JHC, Wong MH. Effects of earthworm activity and P-solublising bacteria on P availability in soil. Journal of Plant Nutrition and Soil Science. 2004;167:209-213
  135. 135. Orozco FH, Cegarra J, Trujillo LM, Roig A. Vermicomposting of coffee pulp using the earthwormEisenia fetida: Effects on C and N contents and the availability of nutrients. Biology and Fertility of Soils. 1996;22:162-166
  136. 136. Bhat SA, Singh J, Vig AP. Potential utilization of bagasse as feed material for earthworm Eisenia fetida and production of vermicompost. Spring. 2015;4:11. DOI: 10.1186/s40064-014-0780-y
  137. 137. Elvira C, Sampedro L, Benitez E, Nogales R. Vermicomposting of sludges from paper mill and dairy industries withEisenia andrei: A pilot scale study. Bioresource Technology. 1998;63:205-211
  138. 138. Delgado M, Bigeriego M, Walter I, Calbo R. Use of California red worm in sewage sludge transformation. Turrialba. 1995;45:33-41
  139. 139. Suthar S. Potential utilization of guar gum industrial waste in vermicompost production. Bioresource Technology. 2006;97:2474-2477
  140. 140. Nriagu JO. Global metal pollution-poisoning the biosphere? Environment. 1990;32:7-11
  141. 141. Brady NC, Weil RR. The Nature and Properties of Soils. 13th ed. New Delhi: Prentice Hall of India; 2002. p. 960
  142. 142. Lasat MM. Phytoextraction of metals from contaminated soil: A review of plant/soil/metal interaction and asessment of pertinent agronomic issue. Journal of Hazardous Substance Research. 2000;2:1-25
  143. 143. Hopkin SP. Ecophysiology of Metals in Terrestrial Invertebrates. London: Elsevier Applied Science; 1989
  144. 144. Paoletti MG, Favretto MR, Stinner BR, Purrington FF, Bater JE. Invertebrates as bioindicators of soil use. Agriculture, Ecosystems and Environment. 1991;34:341-362
  145. 145. Tavares TM, Carvalho FM. Avaliacao de exposicao de populaces humanas a metais pesados no ambiente: Exemplos do reconcavo baiano. Quimica Nova. 1992;15:147-154
  146. 146. Lukkari T, Teno S, Vaisanen A, Haimi J. Effect of earthworms on decomposition and metal availability in contaminated soil: Microcosm studies of populations with different exposure histories. Soil Biology & Biochemistry. 2006;38:359-370
  147. 147. Maboeta MS, Reinecke AJ, Reinecke SA. Effects of low levels of lead on growth and reproduction of the Asian earthwormPerionyx excavatus(Oligochaeta). Ecotoxicology and Environmental Safety. 1999;44:236-240
  148. 148. Lukkari T, Taavitsainen M, Nen AVI, Haimia J. Effects of heavy metals on earthworms along contamination gradients in organic rich soils. Ecotoxicology and Environmental Safety. 2004;59:340-348
  149. 149. Fischer E, Molnar L. Environmental aspects of the chloragogenous tissue of earthworm. Soil Biology & Biochemistry. 1992;24:1723-1727
  150. 150. Spurgeon DJ, Hopkin SP. Comparison of metal accumulation and excretion kinetics in earthworms (Eisenia fetida) exposed to contaminated field and laboratory soils. Applied Soil Ecology. 1999;11:227-243
  151. 151. Beyer WN, Cromartie E, Moment GB. Accumulation of Methylmercury in the earthworm,Eisenia fetida, and its effect on regeneration. Bulletin of Environmental Contamination and Toxicology. 1985;35:157-162
  152. 152. Boudou A, Ribeyre F. Mercury in the food web: Accumulation and transfer mechanism. In: Sigel A, Sigel H, editors. Metal Ion in Biological Systems, Vol. 34. NY, USA: Marcel Dekker, Inc.; 1997. pp. 289-319
  153. 153. Burton DT, Turley SD, Fisher DJ, Green DJ, Shedd TR. Bioaccumulatiopn of total mercury and monomethyl mercury in the earthwormEisenia fetida. Water, Air, and Soil Pollution. 2006;170:37-54
  154. 154. Zhang ZS, Zheng DM, Wang QC, Lv XG. Bioaccumulation of total and methyl mercury in three earthworm species (Drawidasp.,Allolobophorasp., andLimnodrilussp.). Bulletin of Environmental Contamination and Toxicology. 2009;83:937-942
  155. 155. Maenpaa KA, Kukkonen JVK, Lydy MJ. Remediation of heavy metal-contaminates soils using phosphorus: Evaluation of bioavailability using an earthworm bioassay. Archieves of Environment Contamination and Toxicology. 2002;43:389-398
  156. 156. Malley C, Nair J, Ho G. Impact of heavy metals on enzymatic activity of substrate and on composting wormsEisenia fetida. Bioresource Technology. 2006;97:1498-1502
  157. 157. Kizilkaya R. The role of different organic wastes on zinc bioaccumulation by earthworm Lumbricus terrestris L.(Oligochaeta) in successive Zn added soil. Ecological Engineering. 2005;25:322-331
  158. 158. Maity S, Padhy PK, Chaudhury S. The role of earthwormLampito mauritii(Kinberg) in amending lead and zinc treated soil. Bioresource Technology. 2008;99:7291-7298
  159. 159. Liua X, Hua C, Zhang S. Effects of earthworm activity on fertility and heavy metal bioavailability in sewage sludge. Environment International. 2005;31:874-879
  160. 160. Udovic M, Lestan D. The effect of earthworms on the fractionation and bioavailability of heavy metals before and after soil remediation. Environmental Pollution. 2007;148:663-668
  161. 161. Sivakumar S, Subbhuraam CV. Toxicity of chromium (III) and chromium (VI) to the earthwormEisenia fetida. Ecotoxicology and Environmental Safety. 2005;62:93-98
  162. 162. Wei-bao S, Hong-qiang Y. Effects of earthworm and microbe on soil nutrients and heavy metals. Agricultural Sciences in China. 2008;7:599-605
  163. 163. Owojori OJ, Reinecke AJ, Rozanov AB. Effects of salinity on partitioning, uptake and toxicity of zinc in the earthwormEisenia fetida. Soil Biology & Biochemistry. 2008;40:2385-2393
  164. 164. Frank R, Klauck C, Stonefield KI. Metal transfer in vermicomposting of sewage sludge and plant wastes. Bulletin of Environmental Contamination and Toxicology. 1983;31:673-679
  165. 165. Deolalikar AV, Mitra A, Bhattacharyee S, Chakraborty S. Effect of vermicomposting process on metal content of paper mill solid waste. Journal of Environment Science Engineering. 2005;47:81-84
  166. 166. Khwairakpam M, Bhargava R. Bioconversion of filter mud using vermicomposting employing two exotic and one local earthworm species. Bioresource Technology. 2009;100:5846-5852
  167. 167. Kang J, Zhang Z, Wang JJ. Influence of humic substances on bioavailability of cu and Zn during sewage sludge composting. Bioresource Technology. 2011;102:8022-8026
  168. 168. Walter I, Martinez F, Cala V. Heavy metals speciation and phytotoxic effects of three representative sewage sludges for agricultural uses. Environmental Pollution. 2006;139:507-514

Written By

Jaswinder Singh, Sharanpreet Singh, Adarsh Pal Vig and Arvinder Kaur

Submitted: October 20th, 2017 Reviewed: February 9th, 2018 Published: June 27th, 2018