1. Introduction
Living systems are open, irreversible systems, determined by inheritance and dependent on temperature and time. They exchange substances with the environment and they need free energy for life. Living systems transform energy and matter during metabolism, which could be described as a controlled capacity to transform energy, by the First Law of Thermodynamics. Nevertheless, energy transformation includes the loss of some free energy as heat, by the Second Law of Thermodynamics, which as a consequence increases disorder - entropy. Plant cells are simultaneously characterized by two opposing types of reactions: endergonic, such as photosynthesis (occurring in green plastids) and exergonic, such as respiration (present in mitochondria). Since the metabolic reactions are controlled, they need activation energy that is provided by biological catalysts – enzymes, which lower the activation energy without its consumption. Nevertheless, the limits in the application of thermodynamics in the biochemistry of living systems are the non-existence of time and total reversibility, as a category, which could be surpassed in plant systems by introducing of temperature sums as important factor of plant development.
A living system assimilates high-enthalpy, low-entropy compounds from its surroundings, transforms them into a more useful form of chemical energy and returns low-enthalpy, high-entropy compounds to environment. From this point of view, a living organism must be ordered and cannot be at equilibrium. Steady state in an open system is the analogue of equilibrium in a closed system. From the standpointof thermodynamics, the normal functions of living systems are enabled by the concomitant presence of two opposing tendencies: the preservation of a steady state and the aspiration to spontaneously transcend a non-equilibrium state. A steady state,
Plants are open systems which can directly use (transform) light energy to convert CO2 and H2O into glucose, which cellular respiration converts into ATP. They reproduce and surviving the unfavourable conditions in the form of seeds. A seed is a living system with a low water content and metabolism reduced to the minimum. It contains genetic information which enables the life of a new plant. The most critical period for a seed is imbibition and the beginning of germination, which represents a shifting of the system from a latent state (steady state). Seed storage (ageing factor) induces qualitative and quantitative changes, which could have as a consequence loss of viability. If deterioration is not significant, the system results in a new plant by rehydration and substance allocation present in processes of hydrolysis and biosynthesis. During its lifetime cycle, a plant dissipates energy gradients from the point of growth and development. Environmental stresses increase the internal entropy of a plant, moving it closer to equilibrium. In response, plants employ repair systems, requiring additional energy for the recovery processes, having as a consequence a lowering of the energy available for work and an increase in entropy. Decrease of entropy of any living system towards equilibrium, having as a consequence death(Shimokawa & Ozawa, 2005).
2. The thermodynamics of seed and the maintenance of seed viability
A seed is a biological system in the state of anhydrobiosis with living processes reduced to the minimum tomaintain the germination ability (viability) - the crucial biological aspect. The term is derived from Greek and indicates “life without water”. Anhydrobiosis is a highly stable state of suspended energy due to desiccation pending recovery by rehydration. This state seems always to be characterized by the cessation of measurable metabolism.
From this point, seed viability could be maintained during long periods owing to their glass structure, which was defined by Buitink & Leprince (2004)as a thermodynamically unstable state, with high viscosity (enabled by low tissue moisture and low temperature), so as the viscosity is so high that diffusional movement is effectively prevented for time periods or practical utility. Sun (2000); Walters (2007); Buitink & Leprince (2008) ascertained that glass stability is not upheld
2.1. Seed structure and water as bearer of equilibrium
Water is of great importance for living systems; either it is a reaction medium or a reactant. The main characteristic of seed is low water content, which could vary depending on plant species, environment and seed condition (Beardmore et al., 2008; Siddiqui et al., 2008). Seed water consists of two components, bound and bulk water (Krishnan et al., 2004c).
Ageing is a characteristic of all living systems, seeds included. Irrespective to fact that vitrification presents a conservation state for seed systems (close to a steady state), silent metabolic processes are present, with a lower ability to counteract developed injuries. This means that vitrification has a double nature: conservation (low entropy and enthalpy) with a low ability of recovery, as opposed to the hydrated state, where the entropy and enthalpy are large with high mobility and high recovery ability.
Based on ability to maintain viability during long periods and to endure dessication, Pammentner & Berjak (1999)divided the seeds of all plant species into two groups:
Orthodox – which could be stored for a long time and endure extreme desiccation;
Recalcitrant - which could not be stored for a long time and could be injured by desiccation.
From the point of glass stability, water and temperature play significant roles in determining the storage longevity of orthodox seeds. Some models have demonstrated that the effects of water and temperature on seed aging are interdependent (Beardmore et al., 2008), indicating desiccation as the main contributor to loss of seed viability. On the other hand, Walters et al. (2001; 2010) considered that seed ageing, as the main result of free radical production, is the most important factor for viability loss. Furthermore, Vertucci (1989) established that an increase of seed moisture over 0.25 g g-1 dry weight increases seed respiration. One of the basic mechanisms in energy transmission during desiccation (induced by ageing) is the redox state of system (Kranner & Birtić, 2005). This gives a more complex view to the maintenance of seed viability.
Desiccation of plant tissues presents a shift of the water from the liquid to the vapour phase (Sun, 2002). Temperature influences evaporation, as well as the partial water vapour pressure in the air and the energy status of water in plant tissue, both in dry and hydrated plant tissue. An increase in temperature results in a decrease in the equilibrium water content at a given relative humidity (water activity) or an increase in the equilibrium water activity for a given tissue water content (Fig. 1). Water activity can be described as the ‘effective’ water content, which is thermodynamically available for various physiological processes in cells. The temperature dependence of the isotherm shift is described by the Clausius-Clapeyron Equation:
where q is the excess heat of sorption;
The structural changes observed during the process of seed ageing consider disturbances of the glass structure (Hoekstra et al., 2001; Walters et al., 2010) and the increase of the oxidative activity (Walters et al., 2001; Dussert et al., 2006) as a consequence of increased respiration.Based on thermodynamics, the change of internal energy of a system represents the maximal work which could be achieved.
The alterations of external (temperature and/or humidity) and internal factors (glass stability) influence the respiration of dry seeds (Walters et al., 2001) and their energy status, which could be calculated by use of the sorption isotherm, as suggested by Vertucci & Leopold (1984):
where, at a given tissue water content,
Irrespective of the fact that the observed experiments were performed on tissue during hydration/dehydration, it is well known that even minimal water content in seeds could have an important function in altering the glassy matrix. Molecular mobility was found to be inversely correlated with storage stability. With decreasing water content, the molecular mobility reached a minimum, but increased again at very low water contents. This correlation suggests that storage stability might be at least partially controlled by molecular mobility (Buitink et al., 2000).
Krishnan et al. (2004b) ascertained that the thermodynamic properties of seed water determine the reaction kinetics during seed deterioration. The thermodynamic properties showed a critical upper limit, with tolerant species having higher values than susceptible species. In general, the values of the critical limits of the thermodynamic parameters decreased with increasing temperature. The differential enthalpy and entropy increased in seeds with period of storage and became asymptotic as the seeds lost their viability. The importance of temperature, as a seed deterioration factor was also emphasised by Dragicevic (2007), with the increased values of the differential free energy found during accelerated ageing of susceptible (sugary genotypes) and tolerant (dent genotypes) maize seeds (Fig. 2). The radical increase in the
2.2. Importance of redox equilibrium
Life depends on a balance between entropy and enthalpy. For plants, the required energy for maintaining an ordered state is achieved by oxidation (respiration) of photosynthesized substances. Mitochondrial respiration provides energy for biosynthesis and its balance with photosynthesis determines the rate of plant biomass accumulation (Millar et al., 2011). The result of oxidation is an overall reducing environment in cells. During oxidation, photo-oxidative stress and photorespiration, the production of reactive oxygen species (ROS) is an unavoidable consequence (Brosche et al., 2010). ROS production requires or releases some quantities of energy (the voltage of an electrochemical cell is directly related to the change of the Gibb’s energy, Fig. 3), which was briefly described by Vitvitskii (1969) and Buettner (1993):
Redox reactions require redox couples which are responsive to electron flow, which contribute to the distinct reducing/oxidizing environment. Sets of redox couples can be independent from other sets if the activation energies for the reactions are high and there are no enzymatic systems to link them kinetically, which is commonly the case in seeds. Schafer & Buettner (2001) defined the redox environment of a linked set of redox couples, as found in a biological fluid, organelle, cell, or tissue, as the summation of the products of the reduction potential and reducing capacity of the linked redox couples present.
The reduction potential can be described as the voltage (reducing capacity) present in the number of available electrons. The reducing capacity could be estimated by determining the concentration of the reduced species in a redox couple using the Nernst equation:
where,
The Nernst equation has a wide range of applications in biology because many biochemical reactions in living organisms involve electron transfer reactions. These reactions are responsible for energy production. The voltage of an electrochemical cell is directly related to the change in the Gibbs energy:
where n is the number of electrons exchanged in the chemical process, Fis the Faraday constant and ∆E is the electromotive force under standard conditions, i.e., the difference in the standard reduction potentials of the two half-cells involved in the process.
Under non-standard conditions, the relationship can be derived from a process such as:
where k is the mass action expression, RT ln k is a “correction” factor, necessary because of the non-standard conditions.
Using Eq. 5, the voltage of an electrochemical cell can be expressed by the Nernst equation:
Understanding the dynamics of the redox elements in biological systems remains a major challenge for redox signalling and oxidative stress research (Schafer & Buettner, 2001). The reduction potential of various redox couples in the cell could be viewed as triggers to activate a cellular switchboard that move the cell through different physiological phases: from proliferation (Fig. 4) through various stages of differentiation and, when stressed or damaged in such a way that the redox environment cannot be maintained, into apoptosis. Necrosis is the complete loss of the ability to activate and/or respond to changes in these nano-switches.
With the exception of stress situations, this phenomenon is connected with planned dismissing of individual parts of an organism, which lose functionality (programmed cell death), such as necrosis of aleurone and seed rest, after shifting to autonomic nutrition (Mrva et al., 2006). This approach represents the first step into a new area of quantitative biology.
The redox couples and quantification of physiological states are not only important for seeds, but also for all phases of any living system. They contribute to stress tolerance, increase the enthalpy of a system, manage development and, as such, the life cycle on the cellular level. From that point of view plants develop a series of pathways at different levels that combat with environmental stress, which produces more ROS (Shao et al., 2008). These pathways include the phtorespiratory pathway, enzymatic and non-enzymatic pathways, corresponding responsive-gene regulation and anatomical ways, which includes drought, salinity, low temperature, UV-B and others.
3. Thermodynamics of germination and plant growth
The introduction of universal thermodynamic parameters could enable a better understanding of the processes of growth and reactions, such ashydrolysisand biosynthesis, incorporated in seed germination. Boyer (1969) quantified water transport into plant based on the energy concept. In addition, the input of water was determined as energy input (Manz et al., 2005; Kikuchi et al., 2006). Recently, Sun (2002) recognized free energy input by water as the presumable factor of plant growth. Moreover, plant growth is also the result of biomass (substance) assimilation and from that point of view Hansen et al.(1998) and Smith et al. (2006) proposed thermodynamic model to describe relation between plant growth and respiration rates (metabolism efficiency). When considered together, simultaneously measured values of CO2 production rate, O2 use rate and metabolic heat rate provide a link between cellular and whole-plant processes. 25 KJ mol-1 is taken as total enthalpy change per mole of carbon incorporated into biomass.
3.1. Germination as the double phase shifting of water
During the imbibition, seeds absorb high water quantities during a relative short period, which depend on the species,
Sun (2002) delineated that water sorption is an exothermic event (Fig. 1). A high negative enthalpy value at low water content suggests the strong affinity of water molecules to polar sites. As the water content increases, the enthalpy becomes less negative. As process based on volume increasing, sorption is temperature dependent category. Moreover, Vertucci & Leopold (1984); Manz et al. (2005); Kikuchi et al. (2006) clarified that the strongest negative enthalpy value occurs at about 7 % water content, and a small negative enthalpy is also observed at moisture contents between 8 % and 25 % (Fig.5). Within this region, the entropy approximates zero. The lack of measurable respiration at moisture contents below 8% is consistent with the lack of activity for most enzymes at such dry conditions. The region between 8 and 25 % moisture has been termed the region of “restricted metabolism”. This is the range in which liquid water first appears and where the differential entropy values indicate the first solution effects. Within this region of hydration, there are great changes in the ability of the seed to endure excessive and rapid imbibition. The enthalpy is low, but still negative. In the final wetting range, at moisture contents between 24 and 32 %, respiration begins to expand rapidly in response to moisture, when resistance to leakage and chilling injury is established. Damage due to imbibing water is the greatest when the initial seed moisture contents are in the region of strongest water binding. Damage is reduced and finally absent when the seed moisture contents are increased to the second and then to the third level of water affinity. The primary hydration process is considered to be completed when the differential enthalpy of hydration approaches zero. The entropy change reflects the relative order and itspeak (Fig. 1 and 5) is presumably associated with the saturation of all primary hydration sites. No consistent differences in the water sorption characteristics has been found between recalcitrant and orthodox seed tissues (Sun, 2000).
Contrary to experimental data realized on viable seeds, Krishnan et al. (2004a, 2004c) established that germinating and non-germinating seeds contained three types of water (bound, bulk and free water) in phase I of hydration. During phase II of hydration, the bulk water of non-germinating seeds disappeared completely, resulting in two types of water. However, three types of water were observed in germinating seeds in phase II. The rapid hydration in phase III was observed only in germinating seeds. The observed data indicate the importance of free water during the imbibition process, as an activator and bearer of the germination process. The water front brings energy into seed, contributing to an increase in the thermal energy necessary for the commencement of endergonic reactions (activation energy).
3.2. Growth as conversion of energy and substance
When biochemical reactions in imbibed seeds attain a critical point, inducing cell division, germination commences, giving as the product a new plant, resulting from the genome which was stored during shorter or longer periods in the seed (embryo). It is important to underline that one of the most important factors in this moment is triggering of different developmental phases by reduction potential-driven nano-switches. The next phase is characterized by water and substance distribution (allocation) from hydrolysed seed substances, denoted as the plant growth - biosynthesis. Although living systems are non-linear thermodynamic systems, which are far removed from equilibrium (Trepagnier et al., 2004), it is necessary to hypothetically define an energy balance for partial phases and processes. This could be enabled through the introduction of the basic processes of germination: hydrolysis and biosynthesis, as well as the status of their free energy, derived from the reaction constants.
where
where
After the imbibition, the initial growth (germination process) is followed by substance conversion: from the hydrolysed seed substance into biosynthesised substance of the root and shoot, since the young plant is not capable to produce its own substance by photosynthesis (chlorophyll has not been synthesised or it quantity is under a critical amount). Regarding the fact that the seedling’s parts grow unequally (Vysotskaya, 2005; Rauf et al., 2007), which is a particular characteristic of the starting phases of germination, so as their free energy is unequally distributed. Experiments on seed ageing (Dragicevic et al., 2007, Dragicevic, 2007) demonstrated that the highest energy potential of 7-day old seedlings was present on the shoot level, then on the seed rest level and finally on the root level. In comparison to energy introduced by the hydration process, the free energy of hydrolysis and particularly of biosynthesis had significantly lower absolute values. Moreover, the consequences of deterioration processes on the seed/seedling level are present in the absorption of high water quantities and it was defined as “water induced growth” by Boyer (1969), which is a negative state for the system. Contrary to the high energy status introduced into the seed/seedling system by water, the free energy of hydrolysis tends to have minimal variations, while the free energy of biosynthesis had values closest to equilibrium, tending upon higher order of biosynthetic reactions. It is important to emphasise that the domination of exergonic reactions (Davies, 1961; Sun, 2002) is important for the release of the necessary energy and the more intensive they are, the greater is the growth potential of the system. According to the Hess Law, free energy is cumulative, irrespective of its origin; hence, all the potential energy present in a plant system is given by the sum of the individual energy states, resulting from the double phase shifting of water and that released from all the hydrolysis and biosynthesis reactions.
After germination, the growth process is followed by less or more intensive water absorption, as well as substance synthesis. Experiments with wheat (Dragicevic et al., 2008) showed that the free energy of hydrolysis increased linearly (Fig. 6), while the free energy of biosynthesis fluctuated, with the coefficient of conversion. The observed non-linearity could indicate a change in the balance between exergonic and endergonic reactions. Moreover, the inputted and released energies are the result of process and reactions which minimize the energies of a given system. In living, as highly hydrated systems, energy is inputted by water and the total energy of the reactions has to be in a stable equilibrium.
The important points of germination and growth processes aresubstance losses, which Tukey (1970) defined as devastated substance. This substance includes losses through respiration (Kuzyakov &Larionova, 2005; Hill et al., 2007), as well as exudates of roots (Tukey, 1970; Jones et al., 2009) and it is calculated as illustrated in Eq. 8. The leaching (devastation) of hydrolysed substance from a seed-seedling system also means the permanent lost of energy and it starts with imbibition. Sredojevic et al. (2008) established that for soybean about 36 % and for sunflower about 46 %of free energy was lost between the 1st and 8th, i.e., 10th day, of the germination process by the leaching process alone.
The rate of plant growth is proportional to the product of the metabolic rate and the metabolic efficiency for the production of anabolic products. Over much of the growth temperature range, the metabolic rate is proportional to the mean temperature and the efficiency is proportional to the reciprocal temperature variability (Criddle et al., 2005), what could be considered as improvement in understanding of energy and biomass conversions, according to previous model purposed by Hansen et al.(1998) and Smith et al. (2006).
3.3. Growth as a flow process
In living systems, the largest flow of water is from soil, through plants to the atmosphere, the so-called by Yeo & Flowers (2007) soil-plant-atmosphere continuum. The driving force for this water continuum is the difference in free energy between liquid water in the soil and water vapour in the atmosphere. The driving forces diverge upon the different parts of the system. Water movement in soil depends on depth of the soil profile and on the forces that bind water in the capillaries (between soil particles). Water can move in plants through a matrix composed of capillaries (the cell walls) or by tubes (the xylem and phloem) where bulk flow of water occurs under pressure gradients. Water movement between cells depends on the properties of the membranes, which are differentially permeable.
In plants, water moves passively by the potential gradient of water (through semi-permeable membranes) or the potential gradient of pressure (without semi-permeable membranes). Solutes moving across semi-permeable membranes due to the potential gradients of water, combining both: the solute potential and the hydrostatic pressure, with decreasing of free energy of water.
Plants can change intracellular solute potentials, thereby influencing water flow. Water can move in a plant against its water potential only when coupled to the movement of solutes, decreasing the free energy of the solute and when the general net change of free energy (solute and water) is negative. Flow across membranes is passive in response to differences in the water potential and occurs primarily through aquaporins(integral membrane proteins) rather than directly through an impermeable membrane. Aquaporins can be gated reversibly, so that plants may be able to control their plasma membrane water permeability.
Transportation of an uncharged solute uphill against its concentration gradient (Taiz & Zeiger, 2010) from lower to higher concentrations decreases entropy and requires the input of free energy:
The change in free energy when moving one mole of a substance or ion against the membrane potential when considering the work required or performed arises from both the voltage (“electro“) and concentration (“chemical”) gradients.
Ions tend to flow from areas of higher to lower electrochemical potential so that ΔG is negative, which defines the maximal work output a reaction can perform. Cellular functions are ultimately linked to metabolic fluxes brought about by thousands of chemical reactions and transport processes.
The direction of flux is dictated by the change of Gibbs free energy which can be expressed through the thermodynamic equilibrium constant Kjequ as follows (Holzhutter, 2004):
where
With accumulating concentrations of the reaction products (appearing in the nominator) and/or vanishing concentrations of the reaction substrates (appearing in the denominator) (Eq. 13), the concentration-dependent term (Eq. 14) may assume negative values, i.e., in principle, the direction of a chemical reaction can always be reverse provided that other reactions in the system are capable of accomplishing the required change in the concentration of the reactants. From this point of view, the introduction of irreversible thermodynamics (Yeo & Flowers, 2007) imposes upon research of biological fluxes, as more general view, which includes various forces. It applies the parameters used in classical thermodynamics to non-equilibrium conditions, i.e. in situations where there is the net flux of a substance, although the system must be close to equilibrium.
4. Conclusion
Plants are non-linear systems determined by inheritance and dependent on temperature and time, they exist owing to the opposing metabolic processes:
Those which consume energy and produce substances (photosynthesis);
Those which utilize substances and release stored energy to increase enthalpy, as well as to maintain their own status close to a steady state, which increases entropy.
Every plant attempts to maintain structure with the minimal expenditure of energy: they are able to conserve energy in substances; hence they form structures with stored energy to surpass negative conditions and to increase their reproduction ability. The consequence is the formation of seeds in higher plants.
A seed is a biological system in a vitrified state. Regardless of the fact that vitrification presents conservation state for seed systems (close to a steady state), silent metabolic processes, with lower abilities to counter developed injuries, are present. This means that vitrification has a double nature: conservation (low entropy and enthalpy) with low ability of recovery. Long storage could induce deterioration, which is related to changes in the internal energy of the system. In general, the values of the critical limits of the thermodynamic parameters decrease with increasing temperature. The differential enthalpy and entropy increase in seeds with storage time and became asymptotic as the seeds lose their viability. A radical drop in germination follows the trend of ∆G increase and ∆H decrease (with values < 0J mol–1), indicating intensification of endergonic reactions, as well as a parallel shift of the system from a relatively ordered to a random state.
One of the important factors present during any deterioration or stress is electron transfer, inducing the production of free radicals, which are responsible for energy production. A reduction of the potential of various redox couples in a cell could be viewed as a trigger to activate cellular switchboards that move the cell from proliferation through various physiological stages into apoptosis and finally, necrosis (equilibrium). The redox couples are important for all living systems, vitrified or hydrated: they contribute to stress tolerance, and manage development and by this, the life cycle on a cellular level.
The germination process requires energy, which is provided by the environmental temperature and, in the next step, by the energy of the double phase shifting of the water front, under defined temperature conditions.The entered and in this way produced energy activates a whole range of reactions, including hydrolysis and biosynthesis, as concomitant processes, increasing, as a consequence, also the energy. When the biochemical reactions in imbibed seeds reach a critical point, inducing cell division, germination commences, giving as a product a new plant. The next phase is characterized by the distribution of water and substances (from hydrolysed seed substance), denoted as the plant growth - biosynthesis. In comparison to the energy introduced by hydration processes, the free energy of hydrolysis and, particularly, of biosynthesis have significantly lower absolute values. The domination of exergonic reactions is the release of the necessary energy and the more intensive they are, the higher is the growth potential of the system.
From that point of view, water with its characteristics, redox signals and substance conversion (including environment) are crucial points of processes providing in plants, they are dependent on energy flow and its transformations in plant systems.
Furthermore, plant growth is provided for by water flow and substance accumulation. The driving force for soil-plant-atmosphere water continuum is the difference in free energy between liquid water in the soil and water vapour in the atmosphere. Cellular functions are ultimately linked to metabolic fluxes which direction is dictated by the change of the free energy, too.
The introduction of universal thermodynamic parameters, as well as irreversible thermodynamics could lead to a better understanding of the growth process and consecutive reactions such as hydrolysis and biosynthesis, as parts of seed germination. The free energy input by water is a presumable factor of plant growth. From this point of view, free energy can be defined by the water volume in seedling/plant, as well as the constants of substance conversion. Namely, energy generation in seedling arises from the double phase shifting of the absorbed water and by its liberation
Acknowledgments
This work was supported by Ministry of Science and Technological Development of Republic of Serbia, Project No. TR31068, “Improvement of maize and soybean traits by molecular and conventional breeding”.
References
- 1.
Beardmore T. Wang B. S. P. Penner M. Scheer G. 2008 Effects of Seed Water content and Storage Temperature on the Germination Parameters of White Spruce, Black Spruce and Lodgepole Pine Seed . ,36 2 (May 2008),171 185 ,1573-5095 - 2.
Benson E.E. 2008 Cryopreservation of Phytodiversity: A Critical Appraisal of Theory & Practice . ,27 3 (May 2008),141 219 ,1549-7836 - 3.
Boyd N. S. Van Acker R. C. 2004 Imbibition Response of Green Foxtail, Canola, Wild Mustard, and Wild Oat Seeds to Different Osmotic Potentials . ,82 6 (June 2004),801 806 ,1916-2804 - 4.
Boyer J. S. 1969 Measurement of Water Status of Plants. ,20 (June 1969)351 364 ,0066-4294 - 5.
Bryant G. Koster K. L. Wolfe J. 2001 Membrane Behavior in Seeds and Other Systems at Low Water Content: The Various Effects of solutes.11 1 March 2001),17 25 ,0960-2585 - 6.
Brosche M. Overmyer K. Wrzaczek M. Kangasjarvi J. Kangasjarvi S. 2010 Stress Signalling III: Reactive Oxygen Species (ROS). In: ion, A. Pareek, S.K. Sopory, H.J. Bohnert. (Ed.)91 102 , Springer,978-9-04813-112-9 Dordrecht, Netherlands - 7.
Buettner G. R. 1993 The Packing Order of Free Radicals and Antioxidants: Lipid Peroxidation, α-tocopherol, and Ascorbate. ,300 2 (February 1993),535 543 ,0003-9861 - 8.
Buitink J. Hoekstra F. A. Hemminga M. A. 2000 MolecularMobility in the Cytoplasm of Lettuce Radicles Correlates with Longevity. ,10 3 (September 2000),285 292 ,0960-2585 - 9.
Buitink J. Leprince O. 2004 Glass Formation in Plant Anhydrobiotes: Survival in the Dry State. ,48 3 (June 2004),215 228 ,0011-2240 - 10.
Buitink J. Leprince O. 2008 Intracellular Glasses and Seed Survival in the Dry State . ,331 10 (October 2008),788 795 ,1631-0691 - 11.
Copeland L. O. Mc Donald M. B. 2001 Seed germination In: , 4th Ed.,72 123 , Kluwer Academic Publishers Group, Dordrecht, Netherlands,0-79237-322-7 - 12.
Criddle R. S. Hansen L. D. Smith B. N. Macfarlane C. Church J. N. Thygerson T. Jovanovic T. Booth T. 2005 Thermodynamic Law for Adaptation of Plants to Environmental Temperatures ,77 8 (August 2005),1425 1444 ,1365-3075 - 13.
Davies D. D. 1961 Bioenergetics. In: , Cambridge Monographs In Experimental Biology,11 , T.A. Bennet-Clark, P.B.M.G. Salt, C.H. Waddington, V.B. Wigglesworth (Ed),35 52 Cambridge University Press., London, Great Britain - 14.
Dragicevic V. 2007 The Influence of Accelerated Ageing and Stimulative Concentrations of2 4 -D on Maize ( L.) Seeds. PhD, Agricultural Faculty, Univerity of Novi Sad, Serbia, (December 2007), UDC: 633.15:581.48:57.017.6(043.3) - 15.
Dragicevic V. Sredojevic S. Djukanovic L. Srebric M. Pavlov M. Vrvic M. 2007 The Stimulatory Effects of 2,4-D as Hormetic on Maize Seedling’s Growth. 52 3 2007):307 EOF -310,0025-6153 - 16.
Dragicevic V. Sredojevic S. Milivojevic M. 2008 Dependence of Starting Wheat Growth on Dynamics of Hydrolysis, Biosynthesis and Free Energy. , Proceedings,409 411 ,978-8-68247-516-3 Belgrade, Serbia, 24-26 September, 2008 - 17.
Dussert S. Davey M. W. Laffargue A. Doulbeau S. Swennen R. Etienne H. 2006 Oxidative Stress, Phospholipid Loss and Lipid Hydrolysis During Drying and Storage of Intermediate Seeds . ,127 2 (June 2006),192 204 ,1399-3054 - 18.
Gladyshev G. P. 2010 Thermodynamics and Life. ,1 6 10 ,1819-5733 - 19.
Hansen L. D. Smith B. N. Criddle R. S. 1998 Calorimetry of Plant Metabolism: A Means to Rapidly Increase Agricultural Biomass Production .70 3 687 694 ,1365-3075 - 20.
Hatanaka R. Sugawara Y. 2010 Development of Desiccation Tolerance and Vitrification by Preculture Treatment in Suspension-Cultured Cells of the Liverwort Marchantia polymorpha . ,231 4 (March 2010),965 976 ,0032-0935 Heimburg, T. (2010). Lipid Ion Channels. Biophysical Chemistry, Vol. 150, No. 1-3, (August 2010), pp. 2-22, ISSN: 21530378 - 21.
Hill P. Kuzyakov Y. Jones D. Farrar J. 2007 Response of Root Respiration and Root Exudation to Alterations in Root C Supply and Demand in Wheat . ,291 1-2 , (February 2007),131 141 ,1573-5036 - 22.
Hoekstra F. A. Golovina E. A. Buitink J. 2001 Mechanisms of Plant Desiccation Tolerance. ,6 9 (September 2001),431 438 ,1360-1385 - 23.
Holzhütter H. G. 2004 The Principle of Flux Minimization and its Application to Estimate Stationary Fluxes in Metabolic Networks .271 14 (July 2004)2905 2922 ,1742-4658 - 24.
Jones D. L. Nguyen C. Finlay R. D. 2009 Carbon Flow in the Rhizosphere: Carbon Trading at the Soil-Root Interface . ,321 1-2 , (August 2009),5 33 ,1573-5036 - 25.
Kikuchi K. Koizumi M. Ishida N. Kano H. 2006 Water Uptake by Dry Beans Observed by Micro-magnetic Resonance Imaging . ,98 3 (September 2006),545 553 ,1095-8290 - 26.
Kranner I. Birtić S. 2005 A Modulating Role for Antioxidants in Desiccation Tolerance. ,45 5 (November 2005),734 740 ,1557-7023 - 27.
Krishnan P. Joshi D. K. Nagarajan S. Moharir A. V. 2004a Characterization of Germinating and Non-Viable Soybean Seeds by Nuclear Magnetic Resonance (NMR) Spectroscopy . ,14 4 December 2004),355 362 ,0960-2585 - 28.
Krishnan P. Nagarajan S. Moharir A. V. 2004b Thermodynamic Characterisation of Seed Deterioration during Storage under Accelerated Ageing Conditions . Vol. 89,4 (December 2004),425 433 ,1537-5129 - 29.
(2004c)Krishnan P. Joshi D. K. Nagarajan S. Moharir A. V. (2004c Characterisation of Germinating and Non-Germinating Wheat Seeds by Nuclear Magnetic Resonance (NMR) Spectroscopy . ,33 33 1 February 2004)76 82 ,1432-1017 - 30.
Kuzyakov Y. . Larionova A. A. 2005 Root and Rhizomicrobial Respiration: A Review of Approaches to Estimate Respiration by Autotrophic and Heterotrophic Organisms in Soil . ,168 4 (August2005),503 EOF 520 EOF ,1522-2624 - 31.
Manz B. Müller K. Kucera B. Volke F. Leubner-Metzger G. 2005 Water Uptake and Distribution in Germinating Tobacco Seeds Investigated in Vivo by Nuclear Magnetic Resonance Imaging . ,138 3 July 2005),1538 1551 ,1532-2548 - 32.
Millar H. Whelan J. Soole K. L. Day D. A. 2011 Organization and Regulation of Mitochondrial Respiration in Plants. ,62 (June 2011), in press1543-5008 - 33.
Mrva K. Wallwork M. Mares D. J. 2006 α-Amylase and Programmed Cell Death in Aleurone of Ripening Wheat Grains. ,57 4 (March 2006),877 885 ,1460-2431 - 34.
Nascimento W. M. 2003 Preventing Thermoinhibition in a Thermosensitive Lettuce Genotype by Seed Imbibition at Low Temperature . ,60 3 (July/September 2003)477 480 ,0103-9016 - 35.
Osborne D. J. Boubriak I. Leprince O. 2002 Rehydration of Dried Systems: Membranes and the Nuclear genome, In: , Black M. & Pritchard H. W. (Ed.),343 366 , CABI Publishing, New York, USA,0-85199-534-9 - 36.
Pammentner N. W. Berjak P. 1999 A Review of Recalcitrant Seed Physiology in Relation to Desiccation Tolerance Mechanisms.9 1 (January 1999),13 37 ,0960-2585 - 37.
Rauf M. Munir M. ul Hassan. M. Ahmad M. Afzal M. 2007 Performance of Wheat Genotypes under Osmotic Stress at Germination and Early Seedling Growth Stage. ,6 8 (April 2007),971 975 ,1684-5315 - 38.
Sánchez-Nieto S. Enríquez-Arredondo C. Guzmán-Chávez F. Hernández-Muñoz R. Ramírez J. Gavilanes-Ruíz M. 2011 Kinetics of the H+-atpase from Dry and 5-Hours-Imbibed Maize Embryos in its Native, Solubilized, and Reconstituted Forms.4 2 March 2011) pp. In Press,1752-9867 - 39.
Schafer F. Q. Buettner G. R. 2001 Redox Environment of the Cell as Viewed Through the Redox State of the Glutathione Disulfide/Glutathione Couple. 30 11 (June 2001),1191 1212 ,0891-5849 - 40.
Shao H. B. Chu L. Y. Lu Z. H. Kang C. M. 2008 Primary antioxidant free radical scavenging and redox signaling pathways in higher plant cells. ,4 1 8 14 ,1449-2288 - 41.
Shimokawa S. Ozawa H. 2005 Thermodynamics of the Ocean Circulation: A Global Perspactive on the Ocean System and Living Systems. In: , A. Kleidon, R. Lorenz, R. D. Lorenz (Ed.),121 134 , Springer-Verlag, Germany,3-54022-495-5 - 42.
Siddiqui S. U. Ali A. Chaudhary M. F. 2008 Germination Behavior of Wheat (Triticum Aestivum) Varieties to Artificial Ageing under Varying Temperature and Humidity . ,40 3 (Jume 2008),1121 1127 ,2070-3368 - 43.
Smith B. N. Harris L. C. Keller E. A. Gul B. Ajmal Khan. M. Hansen L. D. 2006 Calorespirometric Metabolism and Growth in Response to Seasonal Changes of Temperature and Salt. In:Ecophysiology of High Salinity Tolerant Plants , M.A. Khan and D.J. Weber (Ed.),115 125 , Springer, Netherlands,978-1-40204-018-4 - 44.
Sredojevic S. Dragicevic V. Srebric M. Peric V. Nisavic A. Djukanovic L. 2008 The Quantitatrive Determination of Seed Mass Defect During Germination. 1. The Daily Dynamics of Net Supplemental Free Energy. Journal of Scientific Agricultural Research,69 4 63 77 ,0354-5695 - 45.
Sun W. Q. 2000 Dielectric Relaxation of Water and Water-Plasticized Biomolecules in Relation to Cellular Water Organization, Cytoplasmic Viscosity and Desiccation Tolerance in Recalcitrant Seed Tissues. ,124 3 November 2000),1203 1215 ,1532-2548 - 46.
Sun W. Q. 2002 Methods for the Study of Water Relations under Desiccation Stress, In: , Black M. & Pritchard H. W. (Ed.),47 91 , CABI Publishing, New York, USA,0-85199-534-9 - 47.
Taiz L. Zeiger E. 2010 Solute Transport. Chapter 6, In:Plant Physiology , Fifth Edition87 108 , Sinauer Associates, Sunderland, USA,978-0-87893-507-9 - 48.
Taylor,G.B. 2004 Effect of Temperature and State of Hydration on rate of Imbibition in Soft Seeds of Yellow Serradella . ,55 1 39 45 ,0004-9409 - 49.
Trepagnier E. H. Jarzynski C. Ritort F. Crooks G. E. Bustamante C. J. Liphardt J. 2004 Experimental Test of Hatano and Sasa’s Noequilibrium Steady-State Equality. Proceedings of the National Academy of Sciences,101 42 (19 October 2004),15038 15041 ,0027-8424 - 50.
Tukey H. B. 1970 The Leaching of Substances From Plants .21 305 324 0066-4294 - 51.
Vertuci C. W. Leopold A. C. 1984 Bound Water in Soybean Seed And its Relation to Respiration and Imbibitional Damage.75 1 (January 1984),114 117 ,1532-2548 - 52.
Vitvitskii A. I. 1969 Activation Energy of Some Free-Radical Exchange Reactions . ,5 3 (May 1969),276 278 ,0157-3935 X - 53.
Volk G. M. Crane J. Caspersen A. M. Hill L. M. Gardner C. Walters C. 2006 Massive Cellular Disruption Occurs during Early Imbibition of Cuphea Seeds Containing Crystallized Triacylglycerols . ,224 6 (November 2006)1415 1426 ,0032-0935 - 54.
Vysotskaya L. B. 2005 Mechanisms Coordinating Wheat Seedling Growth Response as Affected by Shoot/Root Ratio . ,52 5 679 684 ,1021-4437 - 55.
Walters C. 2007 Glass Formation, Glass Fragility, Molecular Mobility and Longevity of Germplasm Stored at cryogenic Temperatures. ,55 3 (December 2007),357 358 ,0011-2240 - 56.
Walters C. Pammenter N. W. Berjak P. Crane J. 2001 Desiccation Damage, Accelerated Ageing and Respiration in Desiccation tolerant and Sensitive Seeds . ,11 2 (Jun 2001)135 148 ,0960-2585 - 57.
Walters C. Ballesteros D. Vertucci V. A. 2010 Structural Mechanics of Seed Deterioration: Standing the Test of Time . ,179 6 (December 2010),565 573 ,0168-9452 - 58.
Yeo A. R. Flowers T. J. 2007 The driving forces for water ans solute movement. In: Plant Solute Transport, Yeo, A.R. & Flowers, T.J. (Ed.),29 46 , Blackwell Publishing, Oxford, UK,978-1-4051-3995-3