",isbn:"978-1-80356-963-5",printIsbn:"978-1-80356-962-8",pdfIsbn:"978-1-80356-964-2",doi:null,price:0,priceEur:0,priceUsd:0,slug:null,numberOfPages:0,isOpenForSubmission:!0,isSalesforceBook:!1,isNomenclature:!1,hash:"8eeb7ab232fa8d5c723b61e0da251857",bookSignature:"Dr. Soumen Dhara and Dr. Gorachand Dutta",publishedDate:null,coverURL:"https://cdn.intechopen.com/books/images_new/11513.jpg",keywords:"Fabrication Technologies, Applications, Characterizations, Case Studies, Various Gas Sensors, Improvement of Lifestyle, Societal Benefit, Bio-Sensors, Bioreceptor Molecules, Integration, Packaging, Lab-on-Chip",numberOfDownloads:null,numberOfWosCitations:0,numberOfCrossrefCitations:null,numberOfDimensionsCitations:null,numberOfTotalCitations:null,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"April 8th 2022",dateEndSecondStepPublish:"June 17th 2022",dateEndThirdStepPublish:"August 16th 2022",dateEndFourthStepPublish:"November 4th 2022",dateEndFifthStepPublish:"January 3rd 2023",dateConfirmationOfParticipation:null,remainingDaysToSecondStep:"23 days",secondStepPassed:!1,areRegistrationsClosed:!1,currentStepOfPublishingProcess:2,editedByType:null,kuFlag:!1,biosketch:"A pioneering researcher in nanowire heterostructures and laser spectroscopy, recipient of JSPS (Govt. of Japan) and NPDF (Govt. of India) fellowships, and member of MRS(USA), MRS(India), IPA(India).",coeditorOneBiosketch:"Assistant Professor with the School of Medical Science and Technology, Indian Institute of Technology Kharagpur with research interests that include the design and characterization of portable biosensors, biodevices, and sensor interfaces for miniaturized systems and biomedical applications for point-of-care testing.",coeditorTwoBiosketch:null,coeditorThreeBiosketch:null,coeditorFourBiosketch:null,coeditorFiveBiosketch:null,editors:[{id:"196334",title:"Dr.",name:"Soumen",middleName:null,surname:"Dhara",slug:"soumen-dhara",fullName:"Soumen Dhara",profilePictureURL:"https://mts.intechopen.com/storage/users/196334/images/system/196334.jpeg",biography:"Dr. Dhara received his Ph. D in Physics in 2012 from Indian Institute of Technology Guwahati, India. Presently, he is associated with the Faculty of Science, Sri Sri University, India as an Assistant Professor in Physics. Prior to joining the current\naffiliation, he was a postdoctoral fellow at different renowned institutions, Kobe University Japan, S. N. Bose National Centre for Basic Sciences, India and Cardiff University, United Kingdom. He was awarded prestigious JSPS postdoctoral fellowship based on his research contribution on semiconducting nanowires. He has published more than 32 research articles including 1 review article in high profile international journals and 3 book chapters to his credit. His research trust areas of interests are semiconductor nanostructures, optoelectronics, solid state lighting and light sensors, spectroscopy of nanomaterials, thin-film transistors (TFTs) etc.",institutionString:"Sri Sri University",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"2",totalChapterViews:"0",totalEditedBooks:"0",institution:{name:"Sri Sri University",institutionURL:null,country:{name:"India"}}}],coeditorOne:{id:"442408",title:"Dr.",name:"Gorachand",middleName:null,surname:"Dutta",slug:"gorachand-dutta",fullName:"Gorachand Dutta",profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:"Dr. Gorachand Dutta, PhD is an Assistant Professor with the School of MedicalScience and Technology, Indian Institute of Technology Kharagpur. His research interests include the design and characterization of portable\r\nbiosensors, biodevices and sensor interfaces for miniaturized systems and biomedical applications for point-of-care testing. He received his Ph.D in Biosensor and Electrochemistry from Pusan National University, South Korea,\r\nwhere he developed different class of electrochemical sensors and studied the electrochemical properties of gold, platinum, and palladium based metal electrodes. He completed his Post-doctoral fellowships in the Department of\r\nMechanical Engineering, Michigan State University, USA and Department of Electronic and Electrical Engineering at University of Bath, UK. He has expertise on label-free multichannel electrochemical biosensors, electronically\r\naddressable biosensor arrays, aptamer- and DNA-based sensors and surface bio-functionalization.",institutionString:"Indian Institute of Technology Kharagpur",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"0",totalChapterViews:"0",totalEditedBooks:"0",institution:{name:"Indian Institute of Technology Kharagpur",institutionURL:null,country:{name:"India"}}},coeditorTwo:null,coeditorThree:null,coeditorFour:null,coeditorFive:null,topics:[{id:"11",title:"Engineering",slug:"engineering"}],chapters:null,productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"},personalPublishingAssistant:{id:"429341",firstName:"Paula",lastName:"Gavran",middleName:null,title:"Ms.",imageUrl:"//cdnintech.com/web/frontend/www/assets/author.svg",email:"paula@intechopen.com",biography:null}},relatedBooks:[{type:"book",id:"10198",title:"Response Surface Methodology in Engineering Science",subtitle:null,isOpenForSubmission:!1,hash:"1942bec30d40572f519327ca7a6d7aae",slug:"response-surface-methodology-in-engineering-science",bookSignature:"Palanikumar Kayaroganam",coverURL:"https://cdn.intechopen.com/books/images_new/10198.jpg",editedByType:"Edited by",editors:[{id:"321730",title:"Prof.",name:"Palanikumar",surname:"Kayaroganam",slug:"palanikumar-kayaroganam",fullName:"Palanikumar Kayaroganam"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"1591",title:"Infrared Spectroscopy",subtitle:"Materials Science, Engineering and Technology",isOpenForSubmission:!1,hash:"99b4b7b71a8caeb693ed762b40b017f4",slug:"infrared-spectroscopy-materials-science-engineering-and-technology",bookSignature:"Theophile Theophanides",coverURL:"https://cdn.intechopen.com/books/images_new/1591.jpg",editedByType:"Edited by",editors:[{id:"37194",title:"Dr.",name:"Theophile",surname:"Theophanides",slug:"theophile-theophanides",fullName:"Theophile Theophanides"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"3161",title:"Frontiers in Guided Wave Optics and Optoelectronics",subtitle:null,isOpenForSubmission:!1,hash:"deb44e9c99f82bbce1083abea743146c",slug:"frontiers-in-guided-wave-optics-and-optoelectronics",bookSignature:"Bishnu Pal",coverURL:"https://cdn.intechopen.com/books/images_new/3161.jpg",editedByType:"Edited by",editors:[{id:"4782",title:"Prof.",name:"Bishnu",surname:"Pal",slug:"bishnu-pal",fullName:"Bishnu Pal"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"371",title:"Abiotic Stress in Plants",subtitle:"Mechanisms and Adaptations",isOpenForSubmission:!1,hash:"588466f487e307619849d72389178a74",slug:"abiotic-stress-in-plants-mechanisms-and-adaptations",bookSignature:"Arun Shanker and B. 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\n\t\t\t
1. Introduction
\n\t\t\t
In a world increasingly conscious of the degrading state of its environment and with the surge of the oil prices, renewable energies have found a choice place in the energy supply strategies of a great number of countries.
\n\t\t\t
In fact, the growing interest in clean and durable energies in general, and in the wind energy in particular, is more than one society phenomenon. It presents today a true stake to which the energy supply security and the reduction of the toxic emissions are closely related. That\'s why, the contribution of the backers for the mobilization of the necessary financial and technical resources is a guarantee to support the efforts deployed by our countries in order to control the advanced technologies in this domain.
\n\t\t\t
The African continent disposes of an important potential in renewable energies notably the hydraulics, the solar, the wind, the biogas and the geothermal. However, this potential remains strongly under exploited because of a certain number of obstacles related to the high cost of investment for these systems, the absence of competences and skilled human resources, the limit of the regional co-operation in this domain, the lack of maintenance structures and the absence of information and reliable data on the energy consumptions.
\n\t\t\t
In Tunisia, the development that the country\'s economy has known for these last years with its positive repercussions on the social plan as well as on the living standard has contributed to the acceleration of the energy consumption rhythm, which on average has increased by 4% per year, thus exceeding the development rate of the hydrocarbons production. Indeed, since 2001, Tunisia has become an importer of primary energy (Fig. 1) [1-4].
\n\t\t\t
In the electricity production sector, the Tunisian Company of Electricity and Gas (STEG) has engaged in a diversification of its production park mainly composed of conventional units (vapor thermic, gas turbine and combined cycle), through a progressive recourse to the production projects starting from renewable energies (hydraulic and wind turbines), in spite of their investment cost still high on a worldwide scale (Figs. 2 and 3) [1-4].
\n\t\t\t
The wind power in Tunisia is considered, in the electricity production sector, as a vector carrying in the medium term in contribution to the energy balance improvement and equally to the fight against the climate change. In fact, it is proved that Tunisia is endowed with good wind energy potentials, hardly exploited so far. Moreover, this wind energy currently arouses a great interest not only on behalf of the authorities but also of the private sector. An effort has been made in order to adapt the legal and institutional framework to this orientation [1-4].
\n\t\t\t
Figure 1.
Energy resources and demand in Tunisia.
\n\t\t\t
Currently, the use of the wind energy in Tunisia and, consequently, the installation of wind farms have become unavoidable realities, due to the environmental problems posed by the traditional energy sources and of the aerogenerators technological progress. In fact, in order to meet the country\'s energy needs in the best economic conditions, of quality and respect of the environment as well as the users\' safety, the STEG has already established its first wind park in Sidi Daoud in the area of the Cap Bon, in the North-East of the country (Fig. 4). This power station currently comprises 70 wind turbines of an installed power generation capacity of 53.6 MW, which corresponds to approximately 1 % of the national production park (Table 1). It has been accomplished in three stages [1-4]:
\n\t\t\t
The first section of a power capacity of 10.56 MW, created in 2000, incorporates 32 aerogenerators (Made AE-32) with a asynchronous motor, having the unit nominal power of 330 kW. The second section of power capacity of 8.72 MW, created in 2003, comprises 12 aerogenerators: one wind turbine Made AE-52 with a synchronous motor of 800 kW and 11 wind turbines with asynchronous motor of which one machine Made AE-61 of 1.3MW capacity and 10 machines Made AE-46, each of them with a capacity of 660kW. The third section of power capacity of 34.32 MW, created in 2009, comprises 26 powerful wind turbines (MADE AE-61).
\n\t\t\t
The wind energy station, the object of this study, with these 3 sections is located approximately 5 km of the coastal village of Sidi Daoud (800 inhabitants approximately). It is a sufficiently windy site, able to receive several wind turbines, far from the buildings and the obstacles and close to the electrical supply network (Fig. 5) [1-4].
Wind turbines installation of the Sidi Daoud wind farm - Tunisia.
\n\t\t\t
Figure 4.
Wind farm and electrical network of Sidi Daoud.
\n\t\t\t
The objectives of this study are:
\n\t\t\t
The evaluation of the wind annual characteristics (distribution, direction, characteristic speeds and wind potential) of Sidi Daoud site by the meteorological method and the Weibull and Rayleigh analytical methods [5-21]. The data treated in this study, during four years period (2004-2007), are the measurements recorded in four places of the site (masts 1, 2, 3 and 4) at altitudes which correspond to the heights of the aerogenerators hubs (30, 45, 50 and 60 m above ground level). To evaluate statistically the performances of the Weibull and Rayleigh analytical distributions compared to the experimental distribution, we calculate the statistical parameters analysis for the wind speed and the power density distributions (The determination coefficient R\n\t\t\t\t\t\t2, the chi-square coefficient χ\n\t\t\t\t\t\t2 and the root mean square error (RMSE)) [5-12].
The modeling of the vertical profile of the wind speed in the measurement place mast 4 by the power and logarithmic laws, in order to know the evolution the wind speed at altitudes representing an energy interest [22-27]. A close attention is paid to the study of the influence height on the wind characteristics (mean speed and power density) by using the Rayleigh distribution.
The determination of the energetic performances of the four aerogenerators with horizontal axis MADE AE-32, AE-46, AE-52 and AE-61 installed in site. From its characteristic curves, we study the aerodynamic and energy efficiency in terms of the wind speed, the use factor and the availability rate of each type of aerogenerator, installed with the various masts, and the whole wind farm [28-33].
\n\t\t
\n\t\t
\n\t\t\t
2. Adjustment methods of the meteorological data
\n\t\t\t
During the evaluation of the energetic performance of a wind system, it is essential to study the characteristics of the two elements: the site and the aerogenerator.
\n\t\t\t
The object of the study of the site is to evaluate the following characteristics [5-21]:
\n\t\t\t
Mean speed ;
Most energetic speed ;
Most frequent speed ;
Occurrence frequency ;
Power density ;
Aavailable energy ;
Duration of wind availability ;
Shear coefficient.
\n\t\t\t
The study of the aerogenerator makes it possible to define [28-35]:
Weibull and Rayleigh distribution analytical methods.
\n\t\t\t
From the tables of cumulated frequency of the classified wind speeds, the wind characteristics of the site are given by the following table 2:
\n\t\t\t
The standard deviation enables to study the dispersion of wind speeds measurements around the mean speed. Indeed, if this standard deviation is weak, the values of measurements are regrouped around the average; if it is significant, they are very dispersed.
\n\t\t\t
The annual available energy of the wind in the site per unit area is given by the following relation:
Evaluation methodologies of the wind characteristics.
\n\t\t
\n\t\t
\n\t\t\t
3. Characterization of the site and evaluation of the wind potential
\n\t\t\t
\n\t\t\t\t
3.1. Sidi Daoud site relief
\n\t\t\t\t
The establishment site of this wind station is located between the Mediterranean coasts in the north, of the villages of Sidi Daoud, Ghorman and of the forest dar Chichou in the south and the mountains of El Haouaria in the west coast. It has a mountainous relief, slightly lengthened according to the East–West direction. The vegetation in the neighborhoods of the site is practically uniform and it is composed of trees and shrubs of small sizes. Its geographical coordinates are of 37°02 \' for latitude and of 10°56 \' for longitude. The total area used for the establishment of the power station is approximately 9 ha and extends on 3.5km from the coast (Fig. 6). It is a sufficiently windy site, able to receive several wind turbines, far from the buildings and the obstacles and close to the electrical supply network [1-4].
\n\t\t\t\t
The aerogenerators of the first and the second section are established on the summits of the two mountains "Djebel El Hammam " and " Djebel Ghormane" whose altitude is respectively 50 and 100 m above sea level. The aerogenerators of the third section are located at the bottom of these two hills and about a hundred meters from the marine coasts.
\n\t\t\t\t
Figure 5.
Sidi Daoud site relief.
\n\t\t\t\t
The meteorological data of the Sidi Daoud site used in this study were measured by the technical service of the wind farm during four years (2004–2007). The data relating to the direction and the wind speed were taken by four measurement masts at altitudes which correspond to the heights of the aerogenerators hubs (30, 45, 50 and 60 m above ground level) (Table 3).
\n\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\tMast
\n\t\t\t\t\t\t\t
1
\n\t\t\t\t\t\t\t
2
\n\t\t\t\t\t\t\t
3
\n\t\t\t\t\t\t\t
4
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
Altitude (m)
\n\t\t\t\t\t\t\t
68
\n\t\t\t\t\t\t\t
75.3
\n\t\t\t\t\t\t\t
40.42
\n\t\t\t\t\t\t\t
22.78
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
Height sensor (m)
\n\t\t\t\t\t\t\t
30
\n\t\t\t\t\t\t\t
30
\n\t\t\t\t\t\t\t
45 and 50
\n\t\t\t\t\t\t\t
45 and 60
\n\t\t\t\t\t\t
\n\t\t\t\t\t
Table 3.
Characteristics of the four measurement masts.
\n\t\t\t
\n\t\t\t
\n\t\t\t\t
3.2. Wind roses
\n\t\t\t\t
The wind rose is a spatial representation of the variation of the wind direction for such a site. It illustrates the direction of the dominant winds on a site and enables to plan the wind turbines installation in order to minimize the wake effect caused by nearby obstacles [5-12].
\n\t\t\t\t
\n\t\t\t\t\tFig. 7 represents the wind roses with 36 directions for the various masts. We note the importance of the wind coming from the West and South-East sectors and the wind\'s non-negligible existence from the North-West sector. In addition, the calm wind persists from both the north-east and south sectors.
\n\t\t\t\t
Figure 6.
Wind roses for the various measurement masts.
\n\t\t\t
\n\t\t\t
\n\t\t\t\t
3.3. Wind characteristics: speeds and wind energy estimation
\n\t\t\t\t
The statistical processing of the measured data has made it possible to determine the histograms and their adjustments by the meteorological method and the Weibull and Rayleigh methods, for various masts and heights, whose characteristic elements are:
\n\t\t\t\t
The analysis of table 4 shows that the wind characteristics (speeds and energy at the height 30 m) of mast 1 are better than those of mast 2. Indeed, the wind potential increased by 14% had with the increase the mean wind speed of 3.3% and the most energetic speed of 10%. The parameter k of the Weibull law, which characterizes the frequency distribution form, is about 1.9; whereas the parameter A, which determines the quality of the wind, is better with mast 1 (Figs. 8a and 8b).
The wind characteristics at the height 45 m of mast 4 are slightly superior to those of mast 3 (Table 5). Indeed, the mean speed passes from 6.31m/s (mast 3) to 6.41 m/s (mast 4), which allows an energy profit of 5.6%. The most frequent and the most energetic speeds are respectively 5 m/s and 11 m/s for the two masts.
For Mast 3, the passage of the height 45 m to 50 m allows a gain of 2.5% on the mean speed and 5.73% on the power density.
For Mast 4, the passage of the height 45 m to 60 m allows a gain of 6.4% on the mean speed, 9% on most energetic speed and 20.12% on the power density.
For masts 3 and 4, the parameter k of the Weibull law is about 2 for the various heights, whereas parameter A believes with the height. The distributions of the frequency classified speeds calculated by the two laws are equivalent; which seems normal to us because the form factor k is almost equal to 2 (Figs. 8c, 8d, 8e and 8f).
The standard deviation σ is weak enough, which shows that the measurements are centered on the average (Tables 4 and 5).
\n\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
Mast 1 – 30 m
\n\t\t\t\t\t\t\t
Mast 2 – 30 m
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
Method
\n\t\t\t\t\t\t\t
M
\n\t\t\t\t\t\t\t
W
\n\t\t\t\t\t\t\t
R
\n\t\t\t\t\t\t\t
M
\n\t\t\t\t\t\t\t
W
\n\t\t\t\t\t\t\t
R
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\tVm \n\t\t\t\t\t\t\t\t(m/s)
\n\t\t\t\t\t\t\t
6.59
\n\t\t\t\t\t\t\t
6.68
\n\t\t\t\t\t\t\t
6.59
\n\t\t\t\t\t\t\t
6.38
\n\t\t\t\t\t\t\t
6.45
\n\t\t\t\t\t\t\t
6.38
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\tVf\n\t\t\t\t\t\t\t\t(m/s)
\n\t\t\t\t\t\t\t
5
\n\t\t\t\t\t\t\t
4.97
\n\t\t\t\t\t\t\t
5.26
\n\t\t\t\t\t\t\t
5
\n\t\t\t\t\t\t\t
4.89
\n\t\t\t\t\t\t\t
5.09
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\tVe \n\t\t\t\t\t\t\t\t(m/s)
\n\t\t\t\t\t\t\t
11
\n\t\t\t\t\t\t\t
11.13
\n\t\t\t\t\t\t\t
10.51
\n\t\t\t\t\t\t\t
10
\n\t\t\t\t\t\t\t
10.62
\n\t\t\t\t\t\t\t
10.18
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\tE (kWh/m²/an)
\n\t\t\t\t\t\t\t
1921.61
\n\t\t\t\t\t\t\t
1951.38
\n\t\t\t\t\t\t\t
1737.17
\n\t\t\t\t\t\t\t
1689.79
\n\t\t\t\t\t\t\t
1719.9
\n\t\t\t\t\t\t\t
1578
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\tP (W/m²)
\n\t\t\t\t\t\t\t
219.36
\n\t\t\t\t\t\t\t
222.76
\n\t\t\t\t\t\t\t
198.31
\n\t\t\t\t\t\t\t
192.90
\n\t\t\t\t\t\t\t
196.34
\n\t\t\t\t\t\t\t
180.14
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\tA (m/s)
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
7.52035
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
7.26489
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\tk\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
1.86065
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
1.89609
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\tσ (m/s)
\n\t\t\t\t\t\t\t
3.72887
\n\t\t\t\t\t\t\t
3.72573
\n\t\t\t\t\t\t\t
3.44427
\n\t\t\t\t\t\t\t
3.53762
\n\t\t\t\t\t\t\t
3.53601
\n\t\t\t\t\t\t\t
3.33568
\n\t\t\t\t\t\t
\n\t\t\t\t\t
Table 4.
Wind characteristics of the Sidi Daoud site calculated at the masts 1and 2.
Wind characteristics of the Sidi Daoud site calculated at the masts 3 and 4.
\n\t\t\t\t
Figure 7.
Annual distributions of the wind speed and the available energy for the period 2004-2007.
\n\t\t\t\t
These results prove that the Sidi Daoud site conceals a strong wind potential. Despite the complex relief of the site, the annual wind potential calculated at the various masts for the same height is almost constant, which shows the good stability of the wind resource of the Sidi Daoud site.
\n\t\t\t
\n\t\t\t
\n\t\t\t\t
3.4. Statistical analysis parameters of Weibull and Rayleigh distributions
\n\t\t\t\t
The determination coefficient R\n\t\t\t\t\t2 (R is the correlation coefficient), the chi-square coefficient (χ\n\t\t\t\t\t2) and the root mean square error (RMSE) analysis are statistically calculated to evaluate the performances of Weibull and Rayleigh models. Consequently, a better distribution has the highest value of R\n\t\t\t\t\t2 and the lowest values of RMSE and χ\n\t\t\t\t\t2 [5-21].
\n\t\t\t\t
The R\n\t\t\t\t\t2 gives the effectiveness of the adjustment model. It is much better than its value being nearer to 1. It is calculated as follows:
The χ2 is employed to determine the adjustment quality. At low values of χ2, the better adjustment quality is obtained. It is given by the following relation:
The RMSE also gives the difference between computed and experimental values. Its minimal value tends toward zero. It is defined by the following expression:
The values of these parameters are given in table 6.
\n\t\t\t\t
The comparison of the meteorological distribution (wind speed frequency and power density) with the Weibull and Rayleigh approximations shows that the latter two models present a better adjustment. Indeed, for these two models and for the four measurement masts (Table 6):
\n\t\t\t\t
The determination coefficient R\n\t\t\t\t\t\t\t2 is very near to the unit.
The RMSE is very weak and does not exceed 0.95% for the adjustment of the wind frequency distribution and lower than 2 for the power density distribution.
The chi-square coefficient χ2 is also low and does not exceed 4% for the adjustment of the wind distribution and it varies from 2.7 to the 16.5 for the power density distribution.
\n\t\t\t\t
It is noticed that the two adjustment models are equivalent for masts 3 and 4; which seems normal to us because the Rayleigh distribution is a particular case of Weibull (the form factor k of the studied site is about 2).
\n\t\t\t
\n\t\t\t
\n\t\t\t\t
3.5. Availability duration of wind
\n\t\t\t\t
Another parameter to be considered is the wind availability in the site. The curve speed-duration allows to determine the number of availability hours of the wind speed superior or equal to a given threshold (Fig. 9); it is noticed that the wind blows at a speed higher than V\n\t\t\t\t\tf (V\n\t\t\t\t\tm and V\n\t\t\t\t\te, respectively) about 66% (48% and 11%, respectively) of annual time. Table 7 gives the durations and the minimum power densities for characteristic speeds (V\n\t\t\t\t\tf, V\n\t\t\t\t\tm and V\n\t\t\t\t\te) for various masts and at various heights.
Statistical analysis parameters for the wind speed distribution and the power density distribution relating to the various masts.
\n\t\t\t\t
The curve power-duration also gives the hours’ number when the site has a power density superior or equal to a given threshold (Fig. 10). For example, the site presents a power density higher than 0.5 kW/m² only from 12% (mast 3 at 45 m) to 16% (mast 4 at 60 m) of the annual time.
Duration and minimum power density for the characteristic speeds.
\n\t\t\t
\n\t\t
\n\t\t
\n\t\t\t
4. Vertical extrapolation of the wind speed
\n\t\t\t
\n\t\t\t\t
4.1. Extrapolation laws
\n\t\t\t\t
The precise evaluation of the wind power potential at a site place requires the knowledge of the wind speed at various heights. The standard height of measurement is generally of 10 m, but during a prospection of a site, in order to draw up a wind project, it is preferable to take measures at two or three levels for one period at least six months in order to know the evolution of the wind speed at altitudes representing an energy interest. The majority of work on the determination of the wind vertical profile in the surface boundary layer is based on the similarity theory of Monin-Obukov [22-23]. This theory was supplemented by studies which proposed extrapolation laws of the wind speed of a level H\n\t\t\t\t\t1 on a level H\n\t\t\t\t\t1 according to the variation of roughness classes.
\n\t\t\t\t
In order to draw up a comparative study, two extrapolation laws are retained [22-27]:
\n\t\t\t\t
Logarithmic law ;
Power law.
\n\t\t\t\t
\n\t\t\t\t\t
4.1.1. Logarithmic law
\n\t\t\t\t\t
For neutral atmospheric conditions (i.e. when the turbulence forces are in balance), the Monin-Obukov expression, giving the wind speed profile, is written:
The ground roughness Z\n\t\t\t\t\t\t0 and the corresponding friction speed u\n\t\t\t\t\t\t* are then given starting from the wind speed measurements in two levels H\n\t\t\t\t\t\t1 and H\n\t\t\t\t\t\t2 by the following relations:
\n\t\t\t\t\t\tα is the shear coefficient whose value depends on several factors like roughness, the topography and the atmosphere stability. It is given starting from the speed measurements in two levels H\n\t\t\t\t\t\t1 and H\n\t\t\t\t\t\t2 by the following relation:
To identify the parameters of the site u\n\t\t\t\t\t*, Z\n\t\t\t\t\t0 andα, we applied the two extrapolation laws to mast 4 on the base of the annual mean speed (Table 8). It is noticed that these coefficients correspond to a rough ground with many hedges. Indeed, the Sidi Daoud site has a complex relief and very influenced by the sea (North and South-West sectors) and by the El Haouaria town (South-East and South-West sectors).
\n\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\tu\n\t\t\t\t\t\t\t\t* (m/s)
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\tZ\n\t\t\t\t\t\t\t\t0 (m)
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\tα\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
0.5701
\n\t\t\t\t\t\t\t
0.4977
\n\t\t\t\t\t\t\t
0.2152
\n\t\t\t\t\t\t
\n\t\t\t\t\t
Table 8.
Extrapolation laws parameters calculated at mast 4.
\n\t\t\t\t
The two extrapolation laws applied to the mast 4 have made it possible to trace the variation of the annual mean wind speed with height (Fig. 11). We note that the obtained results perfectly conform for all heights superior than 30 m. The passage of level 30 m at 100 m allows a gain on the mean speed of 30% and an energetic gain of 116%.
\n\t\t\t\t
Figure 10.
Vertical profile of the wind speed and the power density.
\n\t\t\t
\n\t\t
\n\t\t
\n\t\t\t
5. Characterization of installed aerogenerators and evaluation of the energetic efficiencies
\n\t\t\t
\n\t\t\t\t
5.1. Aerodynamic efficiency of the aerogenerators
\n\t\t\t\t
In this part, we are interested in the four types of aerogenerators MADE AE-32, AE-46, AE-52 and AE-61 with horizontal axis, installed in the Sidi Daoud wind farm.
\n\t\t\t\t
According to the technical document of the manufacturer, the characteristics of the machines studied are given by Table 9.
\n\t\t\t\t\tFig. 12. illustrates the variation of the electric power of each machine in function of the wind speed. The machines start from the same speed of 3 m/s (except the AE-32 which begins to 4 m/s) and must stop at 25 m/s. Beyond nominal speed, the power provided by synchronous machine AE-52 remains constant; on the other hand, that provided by asynchronous machines AE-32, AE-46 and AE-61 decreases slightly with the wind speed.
\n\t\t\t\t
Figure 11.
Power curves of the aerogenerators.
\n\t\t\t\t
The aerodynamic efficiency of the wind rotor defined by its power coefficient C\n\t\t\t\t\tp is written:
where \n\t\t\t\t\t\t\n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\tμ\n\t\t\t\t\t\t\t\t\tm\n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\tand \n\t\t\t\t\t\t\n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\tμ\n\t\t\t\t\t\t\t\t\tg\n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\trespectively represent the gearbox efficiency and the generator efficiency.
\n\t\t\t\t
This dimensionless parameter, which expresses the aerodynamic effectiveness of rotor of the various aerogenerators [20-21], is represented by Fig. 13. For such an aerogenerator, this coefficient is a function the wind speed wind, the chock angle and the rotational speed of rotor. The maximum theoretical value of C\n\t\t\t\t\tp given by Betz limit is 59.3%.
\n\t\t\t\t
For the four machines, this coefficient reaches its maximum at the optimal wind speed V\n\t\t\t\t\topt= 9 m/s (Table 11). This maximum varies from 45.51% (AE-61) to 49.07% (AE-32). For low speeds, the curve of the power coefficient progresses quickly towards the optimum operating point. Beyond this point, we observe degradation slower of C\n\t\t\t\t\tp towards a limiting value of the order 4% which corresponds at the cut out speed of the machine.
\n\t\t\t\t
Figure 12.
Curves of aerodynamic efficiency C\n\t\t\t\t\t\t\tp\n\t\t\t\t\t\t\t=f(V) of the various aerogenerators.
\n\t\t\t\t
In addition to the estimate of produced annual energy, it is interesting to know the annual time of the wind turbine production. Fig. 14 illustrates the site frequency-speed histograms and the machines reduced power curve. We observe that during 22 % (respectively 10%, 8% and 9.5%) of the annual time, the wind speed is insufficient to operate the wind turbine AE-32 (respectively AE-46, AE-52 and AE-61) and it blows sufficiently to obtain the full efficiency during 6 % (respectively 2%, 9% and 1.5%) of the annual time. The remaining time of value 72 % (respectively 88%, 83% and 89%), the efficiency varies with the wind speed.
\n\t\t\t\t
Also, we have plotted the power-duration curve of each aerogenerator indicating the time percentage when the wind turbine provides a power higher than a given threshold (Fig. 15). Thus, the machine AE-32 (respectively AE-46, AE-52 and AE-61) will produce its maximum power only for 526 h/year (respectively 175 h/year, 788 h/year and 131 h/year) of the annual time; which accounts for approximately 7.7% (respectively 2.2%, 9.8% and 1.7%) of its operating annual time. We notice that the four aerogenerators most of the time function below their nominal capacities.
\n\t\t\t\t
Figure 13.
Annual frequency–speed histograms of the site.
\n\t\t\t\t
Figure 14.
Annual power–duration curves of the aerogenerators.
\n\t\t\t
\n\t\t\t
\n\t\t\t\t
5.2. Annual energy produced by the various aerogenerators
\n\t\t\t\t
The available energy really usable E\n\t\t\t\t\tu that can be received by the aerogenerator is proportional to the cube of the wind speed and the wind distribution in the site [28-33].
\n\t\t\t\t
Knowing the wind mode, this usable energy is given by the following expression:
where \n\t\t\t\t\t\t\n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\tS\n\t\t\t\t\t\t\t\t=\n\t\t\t\t\t\t\t\tπ\n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\tR\n\t\t\t\t\t\t\t\t\t2\n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\tis the rotor swept surface of radius R.
\n\t\t\t\t
In the same way, recoverable energy E\n\t\t\t\t\tr on the aerogenerator outlet (rotor+gearbox+generator) is given by the machine power curve and the wind statistical distribution.
where \n\t\t\t\t\t\t\n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\tP\n\t\t\t\t\t\t\t\t\ts\n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t(\n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\tV\n\t\t\t\t\t\t\t\t\ti\n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t)\n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t is the electric power on the aerogenerator outlet.
\n\t\t\t\t
We notice that the calculation of recoverable energy by the Weibull and Rayleigh analytical methods necessitates of modeling the power curve P\n\t\t\t\t\ts(V) by an analytical expression. The Boltzman theoretical model allows reproducing this curve correctly. It is written as follows:
The parameters V\n\t\t\t\t\t0, A\n\t\t\t\t\t1\n\t\t\t\t\t, A\n\t\t\t\t\t2 and ω of each aerogenerator are identified by the software "Origin 5.0" and their optimal numerical values are determined by minimizing the quality criterion χ2 (Table 10).
Boltzman theoretical model parameters of the power curve of each aerogenerator.
\n\t\t\t\t
\n\t\t\t\t\tFig. 16 represents the variation of annual energies (available, usable and recoverable) in function of the wind speed for the various masts and aerogenerators. We see that the maxima of the three energies curves pass approximately by the same wind speed, which shows the good adaptation of the aerogenerators to the Sidi Daoud site.
\n\t\t\t\t
We notice that the annual wind power produced by each wind turbine represents approximately one-third of the total available energy in the site.
\n\t\t\t
\n\t\t\t
\n\t\t\t\t
5.3. Energy efficiencies of the aerogenerators
\n\t\t\t\t
Using the computed energies, the wind turbine mean efficiency relating to the available energy is estimated by the expression [28-33]:
These two ratios of energy represent the product of the mechanical efficiency (gearbox and generator) and the rotor aerodynamic efficiency.
\n\t\t\t\t
Figure 15.
Energies curves calculated by the meteorological method.
\n\t\t\t\t
\n\t\t\t\t\tFig. 17 represents the variation of these mean efficiencies as a function of the classified speed for the various aerogenerators. It is noted that the mean efficiencies pass by the same maximum μ\n\t\t\t\t\tmax for a wind speed of approximately 9 m/s. This maximum varies from 41.92 % (AE-61) to 44.8 % (AE-32) (Table 11). It is significant to notice that this mean efficiency remains superior to 0.4 in the wind speed zone included between 6.8 m/s and 11.2 m/s for the AE-32, between 7.7 m/s and 10.25 m/s for the AE-46, between 6.5 m/s and 11.25 m/s for the AE-52 and between 7.8 m/s and 10.45 m/s for the AE-61.
\n\t\t\t\t
Figure 16.
Mean efficiencies curves of the aerogenerators calculated by the meteorological method.
\n\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
Aerogenerators
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\tCpmax\n\t\t\t\t\t\t\t\t (%)
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\tμmax\n\t\t\t\t\t\t\t\t(%)
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\tVopt\n\t\t\t\t\t\t\t\t (m/s)
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
AE-32
\n\t\t\t\t\t\t\t
49.07
\n\t\t\t\t\t\t\t
44.83
\n\t\t\t\t\t\t\t
9
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
AE-46
\n\t\t\t\t\t\t\t
45.77
\n\t\t\t\t\t\t\t
42.05
\n\t\t\t\t\t\t\t
9
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
AE-52
\n\t\t\t\t\t\t\t
47.44
\n\t\t\t\t\t\t\t
42.92
\n\t\t\t\t\t\t\t
9
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
AE-61
\n\t\t\t\t\t\t\t
45.51
\n\t\t\t\t\t\t\t
41.92
\n\t\t\t\t\t\t\t
9
\n\t\t\t\t\t\t
\n\t\t\t\t\t
Table 11.
Optimum operating point of wind turbines.
\n\t\t\t\t
In addition, the annual mean efficiency of each wind turbine is defined by:
The numerical results obtained by the three methods are comparable and indicate that the annual mean efficiency remains higher than 30% for the various machines (Table 12). Consequently, the energy produced by each machine is important and reaches the 1/3 of the site available energy.
\n\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
Aerogenerator
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
AE-32
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
AE-46
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
AE-52
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
AE-61
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
Mast
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
1
\n\t\t\t\t\t\t\t
2
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
3
\n\t\t\t\t\t\t\t
4
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
3
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
4
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
Meteorological
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
29.54
\n\t\t\t\t\t\t\t
31.74
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
31.52
\n\t\t\t\t\t\t\t
30.61
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
32.18
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
30.31
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
Weibull
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
30.53
\n\t\t\t\t\t\t\t
32.10
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
32.45
\n\t\t\t\t\t\t\t
31.73
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
34.63
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
31.09
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
Rayleigh
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
32.96
\n\t\t\t\t\t\t\t
33.75
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
32.75
\n\t\t\t\t\t\t\t
32.32
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
34.53
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
31.64
\n\t\t\t\t\t\t
\n\t\t\t\t\t
Table 12.
Annual mean efficiency μ (in %) of each aerogenerator.
\n\t\t\t\t
In practice, a maximum energy efficiency of wind turbine is ensured by an optimal aerodynamic efficiency of rotor. To optimize this efficiency, the control of the aerogenerator must be made so that the rotational rotor speed adapts to the site wind speed.
\n\t\t\t
\n\t\t\t
\n\t\t\t\t
5.4. Use factor and availability rate
\n\t\t\t\t
However, the wind turbine cannot function with full power all the time (maintenance, breakdowns, wind availability, etc.). To quantify the recovered power by each aerogenerator, it is interesting to calculate its annual use factor UF which is defined by the ratio of the produced electric power on the installed power [28-33]:
According to the relation (16), we note that this factor UF depends only on the wind frequency (at the nacelle height) for such an aerogenerator. Table 13 shows that the machine AE-52, which has the lowest nominal speed (V\n\t\t\t\t\tn=12m/s), presents the best use factor.
\n\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
Aerogenerator
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
AE-32
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
AE-46
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
AE-52
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
AE-61
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
Mast
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
1
\n\t\t\t\t\t\t\t
2
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
3
\n\t\t\t\t\t\t\t
4
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
3
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
4
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
Meteorological
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
26.65
\n\t\t\t\t\t\t\t
25.18
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
24.23
\n\t\t\t\t\t\t\t
24.90
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
27.58
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
26.04
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
Weibull
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
28.00
\n\t\t\t\t\t\t\t
25.92
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
25.01
\n\t\t\t\t\t\t\t
26.22
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
30.08
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
26.79
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
Rayleigh
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
26.90
\n\t\t\t\t\t\t\t
24.99
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
23.70
\n\t\t\t\t\t\t\t
25.19
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
29.04
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
26.11
\n\t\t\t\t\t\t
\n\t\t\t\t\t
Table 13.
Annual use factor UF (in %) of each aerogenerator.
\n\t\t\t\t
Based on the results of the annual energy recovered by each machine, we note that the use factor of the whole wind farm (70 aerogenerators of an installed power generation capacity of 53.6 MW) is about 25.87%; what shows that the maximum annual energy production of the wind power station is approximately 121.5 GWh/an.
\n\t\t\t\t
To estimate the operation duration of an aerogenerator, we define the availability rate AF which depends on the machine characteristics and the wind potential in the site. For such a wind turbine having a cut in speed V\n\t\t\t\t\td and a cut out speed V\n\t\t\t\t\tc, the availability rate AF is the probability P calculated by the following equation [28-33]:
In general, this factor rises when the difference (V\n\t\t\t\t\tc-V\n\t\t\t\t\td) and the mean wind speed increase. The obtained values for the various aerogenerators are excellent (Table 14) and show that the production time exceeds 90% of annual time for machines AE-46, AE-52 and AE-61 and about 80% for the AE-32.
\n\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
Aerogenerator
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
AE-32
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
AE-46
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
AE-52
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
AE-61
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
Mast
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
1
\n\t\t\t\t\t\t\t
2
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
3
\n\t\t\t\t\t\t\t
4
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
3
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
4
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
Meteorological
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
79.01
\n\t\t\t\t\t\t\t
78.24
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
90.18
\n\t\t\t\t\t\t\t
90.76
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
91.86
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
90.86
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
Weibull
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
73.41
\n\t\t\t\t\t\t\t
82.94
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
92.66
\n\t\t\t\t\t\t\t
92.81
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
93.44
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
93.47
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
Rayleigh
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
74.87
\n\t\t\t\t\t\t\t
84.06
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
92.42
\n\t\t\t\t\t\t\t
92.66
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
92.77
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
93.48
\n\t\t\t\t\t\t
\n\t\t\t\t\t
Table 14.
Annual availability rate AF (in %) of each aerogenerator.
\n\t\t\t\t
Consequently, to completely describe the energetic profitability of an aerogenerator, it is necessary to take account simultaneously of these four factors: the aerodynamic efficiency, the mean efficiency, the use factor and the availability rate.
\n\t\t\t
\n\t\t
\n\t\t
\n\t\t\t
6. Conclusion
\n\t\t\t
This study has presented the development of the wind power use in Tunisia for the electricity production. The main contribution of this chapter is the energy performance evaluation of the first wind farm installed in Sidi Daoud - Tuinisia, particularly the effectiveness of various aerogenerators (MADE AE-32, AE-45, AE-52 and AE-61) implanted on the site, by the meteorological experimental method and the Weibull and Rayleigh analytical methods.
\n\t\t\t
The data treated in this study are the measurements recorded in four places (masts 1, 2, 3 and 4) of the site at altitudes which correspond to the heights of the aerogenerators hubs (30, 45, 50 and 60 m above ground level) (Tab. 2). These measurements are spread out over a four-year period (2004-2007).
\n\t\t\t
The principal results of this study are:
\n\t\t\t
Concerning the wind resource of the site,
\n\t\t\t
The Sidi Daoud site has an important and stable wind potential. Indeed, the power density calculated at the various heights (30, 45, 50 and 60 m) varies from 180 to 230 W/m² according to the measurement mast place. The mean speed also varies from 6.3 to 6.8 m/s. The dominant directions of the wind are the west and south-east sectors.
The identified parameters of the two distribution functions (A, k and V\n\t\t\t\t\t\tm) show that the two models are quasi-equivalent. Indeed, the values of the statistical analysis parameters (R², RMSE and χ\n\t\t\t\t\t\t2) indicate a better adjustment of the meteorological data by the two models.
The modeling of the wind vertical profile by the logarithmic and power laws is applied to the mast 4 place. The extrapolation of the height 30 to 100 m enables us to obtain a gain on the mean speed of 30% and a gain on the power density of 116%.
\n\t\t\t
Concerning the aerogenerators performance,
\n\t\t\t
The maximum power coefficient C\n\t\t\t\t\t\tpmax varies from 45.51% (AE-61) to 49.07% (AE-32) for the same optimal wind speed V\n\t\t\t\t\t\topt = 9 m/s.
The annual mean efficiency remains superior to 30% for the various machines. Indeed, recoverable energy is important and it is about the 1/3 of the available energy in the site.
The use factor UF varies from 23 to 28% according to the type and place of the aerogenerator. It is about 25.87% on average for the whole wind farm.
The availability rate AF is excellent and exceeds 90% of annual time for aerogenerators AE-46, AE-52 and AE-61 and about 80% for the AE-32.
The aerogenerator AE-52 presents the energetic performances higher than those of the other machines.
\n\t\t
\n\t\t
\n\t\t\t
7. Nomenclature
\n\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\tV\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
Wind speed (m/s)
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\tF(V)
\n\t\t\t\t\t\t
Cumulated frequency
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\tf(V)
\n\t\t\t\t\t\t
Occurrence frequency
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\tn\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
Number of wind-speed classes
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\tVm\n\t\t\t\t\t\t\t\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
Mean speed (m/s)
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\tVf\n\t\t\t\t\t\t\t\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
Most frequent speed (m/s)
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\tVe\n\t\t\t\t\t\t\t\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
Most energetic speed (m/s)
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\tPd\n\t\t\t\t\t\t\t\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
Power density at Betz limit (W/m²)
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\tEd\n\t\t\t\t\t\t\t\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
Available energy at Betz limit (kWh/m²/year)
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\tEu\n\t\t\t\t\t\t\t\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
Usable energy (kWh/m²/year)
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\tEr\n\t\t\t\t\t\t\t\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
Recoverable energy (kWh/m²/year)
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
Ps\n\t\t\t\t\t\t\t Pn\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
Electric power on the aerogenerator outlet (W) Nominal power of the aerogenerator (W)
Standard deviation calculated from the meteorological, Weibull and Rayleigh methods (m/s)
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\tR2\n\t\t\t\t\t\t\t\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
Determination coefficient
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\tχ2\n\t\t\t\t\t\t\t\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
Chi-square coefficient
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\tRMSE\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
Root mean square error
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\tyi\n\t\t\t\t\t\t\t\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
ith measured value
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\tyic\n\t\t\t\t\t\t\t\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
ith calculated value
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\tym\n\t\t\t\t\t\t\t\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
Mean value
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\tΓ\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
Gamma function
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\tM\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
Meteorological method
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\tW\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
Weibull method
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\tR\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
Rayleigh method
\n\t\t\t\t\t
\n\t\t\t\t
\n\t\t
\n\t\n',keywords:null,chapterPDFUrl:"https://cdn.intechopen.com/pdfs/17122.pdf",chapterXML:"https://mts.intechopen.com/source/xml/17122.xml",downloadPdfUrl:"/chapter/pdf-download/17122",previewPdfUrl:"/chapter/pdf-preview/17122",totalDownloads:3290,totalViews:293,totalCrossrefCites:1,totalDimensionsCites:2,totalAltmetricsMentions:0,impactScore:1,impactScorePercentile:58,impactScoreQuartile:3,hasAltmetrics:0,dateSubmitted:"October 13th 2010",dateReviewed:"May 17th 2011",datePrePublished:null,datePublished:"June 14th 2011",dateFinished:null,readingETA:"0",abstract:null,reviewType:"peer-reviewed",bibtexUrl:"/chapter/bibtex/17122",risUrl:"/chapter/ris/17122",book:{id:"148",slug:"wind-farm-technical-regulations-potential-estimation-and-siting-assessment"},signatures:"Fathi Ben Amar and Mustapha Elamouri",authors:[{id:"25483",title:"Dr.",name:"Fathi",middleName:null,surname:"Ben Amar",fullName:"Fathi Ben Amar",slug:"fathi-ben-amar",email:"Fathi.benamar@ipeis.rnu.tn",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:{name:"University of Sfax",institutionURL:null,country:{name:"Tunisia"}}},{id:"47956",title:"Dr.",name:"Mustapha",middleName:null,surname:"Elamouri",fullName:"Mustapha Elamouri",slug:"mustapha-elamouri",email:"Mustapha.Elamouri@ipeis.rnu.tn",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:null}],sections:[{id:"sec_1",title:"1. Introduction ",level:"1"},{id:"sec_2",title:"2. Adjustment methods of the meteorological data ",level:"1"},{id:"sec_3",title:"3. Characterization of the site and evaluation of the wind potential",level:"1"},{id:"sec_3_2",title:"3.1. Sidi Daoud site relief",level:"2"},{id:"sec_4_2",title:"3.2. Wind roses ",level:"2"},{id:"sec_5_2",title:"3.3. Wind characteristics: speeds and wind energy estimation",level:"2"},{id:"sec_6_2",title:"3.4. Statistical analysis parameters of Weibull and Rayleigh distributions",level:"2"},{id:"sec_7_2",title:"3.5. Availability duration of wind",level:"2"},{id:"sec_9",title:"4. Vertical extrapolation of the wind speed",level:"1"},{id:"sec_9_2",title:"4.1. Extrapolation laws",level:"2"},{id:"sec_9_3",title:"4.1.1. Logarithmic law",level:"3"},{id:"sec_10_3",title:"4.1.2. Power law",level:"3"},{id:"sec_12_2",title:"4.2. Results and comments",level:"2"},{id:"sec_14",title:"5. Characterization of installed aerogenerators and evaluation of the energetic efficiencies",level:"1"},{id:"sec_14_2",title:"5.1. Aerodynamic efficiency of the aerogenerators ",level:"2"},{id:"sec_15_2",title:"5.2. Annual energy produced by the various aerogenerators",level:"2"},{id:"sec_16_2",title:"5.3. Energy efficiencies of the aerogenerators",level:"2"},{id:"sec_17_2",title:"5.4. Use factor and availability rate",level:"2"},{id:"sec_19",title:"6. Conclusion",level:"1"},{id:"sec_20",title:"7. Nomenclature",level:"1"}],chapterReferences:[{id:"B1",body:'\n\t\t\t\t\n\t\t\t\t\tAgence Nationale des Energies Renouvelables (ANER).\n\t\t\t\t\t2003 Etude Stratégique pour le développement des Energies Renouvelables en Tunisie. 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N.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tBrummels\n\t\t\t\t\t\t\tG.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tBuechler\n\t\t\t\t\t\t\tJ.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2007 Wind resource assessment in the state of Arizona: inventory capacity factor and cost. Renewable Energy 32\n\t\t\t\t\t1453 .\n\t\t\t'},{id:"B29",body:'\n\t\t\t\t\n\t\t\t\t\tAbderrazzaq MH.\n\t\t\t\t\t2004 Energy production assessment of small wind farms. Renewable Energy 29\n\t\t\t\t\t2261 .\n\t\t\t'},{id:"B30",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tKavak\n\t\t\t\t\t\t\tE.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tAkpinar\n\t\t\t\t\t\t\tS.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tAkpinar\n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2005 : An assessment on seasonal analysis of wind energy characteristics and wind turbine characteristics. Energy Conversion and Management 46\n\t\t\t\t\t1848 .\n\t\t\t'},{id:"B31",body:'\n\t\t\t\t\n\t\t\t\t\tJohnson GL.\n\t\t\t\t\t2001 Wind energy systems. Electronic Edition. December 10,. Manhattan, KS.\n\t\t\t'},{id:"B32",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tHenderson-Sellers\n\t\t\t\t\t\t\tB.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t1987 Plume rise modelling: the effects of including a wind shear and a variable surface roughness. Ecological Modelling 37\n\t\t\t\t\t269 .\n\t\t\t'},{id:"B33",body:'\n\t\t\t\t\n\t\t\t\t\tCliff WC.\n\t\t\t\t\t1977 The effect of generalized wind characteristics on annual power estimates from wind turbine generators. Report PNL-2436,. US Department of Energy.\n\t\t\t'}],footnotes:[],contributors:[{corresp:null,contributorFullName:"F. Ben Amar",address:null,affiliation:'
Network and Machines Electric Research Unit, INSAT, Tunisia
Preparatory Institute for Engineering Studies of Sfax, Tunisia
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1. Introduction
One of the concerns in environmental matters is the continuous discharge of countless numbers of chemicals derived from human activities into aquatic systems. These include a large number of contaminants, among these commercial and industrial products (e.g., metals, industrial additives, surfactants and pesticides), personal care products, pharmaceuticals and endocrine-disrupting compounds, among others [1]. The presence of relevant concentrations in the environment has dramatic consequences to the organisms that inhabit these systems (e.g., affecting reproduction and survival), which is reflected in the decline of their populations and accumulation of pollutants [2].
A major concern about contaminants in aquatic systems is the bioaccumulation and biomagnification that can result with all organisms present in these systems including harmful effects to human health [3, 4]. Mammalian organisms, especially dolphins, are considered sentinel species for monitoring the health of coastal marine ecosystems [5, 6]. The main reason for that is (1) they are at the highest trophic level of the food chain and due to their role as predators, they can bioaccumulate contaminants, and (2) they also can live for longer periods (more than 40 years). It makes them good organisms to show long-term accumulation characteristics from contaminants like heavy metals in the marine environment [7]. Recently, with the development of new technologies within the “omic sciences” such as genomics, transcriptomics, proteomics and metabolomics, great advances have been made in the biological science disciplines, particularly in human health. In environmental areas “omics” have begun to have a large impact [8], mainly in aquatic toxicology [9, 10]. Together, new genomic sequencing and postgenomic technologies make it possible to obtain detailed information on drugs, toxicants, pollutants, nutrients and physical and psychological stressors on an omic scale [11]. The use of these omic technologies has allowed the emergence of ecotoxicogenomic disciplines [12, 13].
With these technologies, it is possible to determine the effect of a particular event in the life of a cell, organ or organism in response to contaminants. Through the characterization of the transcriptome, proteome or metabolome, one can perform global analysis to determinate transcriptional/proteomic or metabolomic changes at the same time in many samples (cells, tissues, biofluids, etc.) and be able to make the comparison among them. Omics technologies in environmental matters can help to assess the health statuses of aquatic systems, understand the mechanisms of action of the contaminants, through profiling of genes, proteins or metabolites that may enrich key pathways (molecular or biochemical). It sheds light on how dolphins respond to contaminants while helping to predict adverse effects on other marine organisms (Figure 1). This review highlights the omics studies performed on dolphins to gather information regarding contamination levels and their effects on worldwide dolphin populations (Figure 2). Applications of the omics approach help to understand the dolphins’ physiology as a way to monitor dolphin health conditions and to further ecotoxicology studies. Conclusively, it might provide a method for developing regulations for chemical discharge as well as management and conservation strategies for these kinds of ecosystems.
Figure 1.
Integration of omics technologies in marine organisms.
Figure 2.
Number of studies related with omics approach in dolphin.
The three major omics technologies that have proven to have a tremendous impact include transcriptomics, proteomics and metabolomics [14].
2. Selection of bibliographic material
We reviewed relevantly and recently published studies on the applicability and usefulness of Omics in dolphins. The selection of scientific publications was made through the use of search engines from Google Scholar, PubMed and Scopus to locate studies of interest using the keywords “transcriptomic”, “proteomic”, “metabolomic”, “lipidomic” and “dolphin”. We excluded all repeated studies. It was inevitable that some omics research was bot captured due to them not included the keywords we used. For the selection of publications, only the research studies that were related to contaminants were included in the data set (Table 1).
Specie
Omic approach
Type of sample
Contaminant/stressor
Genes/Proteins/Metabolites/Lipids
Contribution
Reference
Transcriptomic
Tursiops truncatus
Microarray (Custom 4x44K Agilent oligo array)
Cultures Skin
Ex vivo assay of BPA (0.1 or 1 μg/ml) and PFOA (0.1 or 1 μg/ml)
BPA: Genes involved response to stress (e.g., programmed cell death protein, tumor suppressor), immune system (e.g., complement factor H, class I histocompatibility alpha chain), lipid metabolism (e.g., adipogenesis regulatory factor, fatty aldehyde dehydrogenase isoform 2), and embryonic development and growth (e.g., titin, nuclear distribution protein nude-like 1). PFOA: Genes involved response to stress (uv excision repair protein rad23 homolog a, heat shock protein 90), immune system (complement c5, acidic mammalian chitinase), embryonic development (vascular endothelial growth factor, Rho GTPase-activating protein)
Genes involved in cell cycle checkpoint and apoptosis, DNA damage and chromatin remodeling (e.g., DDB1, DCN and INO80). Pathway of cellular response to stress
PCBs could cause epigenetic response, DNA damage and chromatin remodeling
Skin from two ecotypes of dolphin: offshore and coastal
HOC
Genes: AHR, CYPIB1, IL16, ESR2, ESRRA, THRA. GO terms: xenobiotic metabolism, immune response, hormone metabolism, DNA repair, and metal binding.
It provides novel insight into contaminant exposure in two bottlenose dolphin ecotypes in the Southern California Bight and highlights potential relationships between HOC exposure and molecular biomarkers
Peripheral blood mononuclear cell (PBMC) from dolphin and human
PFOA y PFOS
In both species: Overexpression of genes linked to inflammation and autoimmune diseases. Difference between species: In human the interferon Signaling pathway is negatively regulated while in dolphin it is positively regulated. Dolphins lack Mx1 and Mx2, key proteins of the Interferon signaling pathway
This study provide a better understanding of the adverse effects of CECs (PFOA, PFOS) on both dolphin and human species
Depict of the most relevant transcriptomic, proteomic and metabolomic studies performed in dolphin in response to the contaminants.
Based on these criteria, 59 publications were selected from >250 reviewed. Transcriptomics was the most frequently applied technique (38%) followed by proteomics (30%), metabolomics (21%) and finally, lipidomic (10.2%). In general searching the omic studies selected, we identified 8 topics including “contamination”, “physiology” and “health” among others based on the type of research described in the publications. More details about each topic are given in Figure 3. Contamination studies were dominant using the transcriptomic method (31%), compared to studies focusing on proteomics (0%), metabolomics (8%) and lipidomics (0%). We noticed that proteomics and lipidomics are less used in studies related to contamination. However, proteomics is the most frequent technology applied to identifying responses associated with the physiology of dolphins (28%), followed by lipidomics (33%), metabolomics (25%), and transcriptomics (13%). With respect to studies related to health, metabolomic tools (34%) were predominant, followed by transcriptomics (26%) and proteomics (11%). Interestingly, we noticed that the number of studies selected in omics and dolphins does not show an increase over time as we expected, it was diverse (Figure 1). After 2016, the selected literature showed an increase in the application of omics in dolphin research, notably, most studies focused on using metabolomics (LC/MS) and transcriptomic high throughput RNA sequencing (RNA-seq) tools as a diagnostic method for the detection of contaminants in oil spills and with contaminants of emerging concern (CECs). In general, it seems that there is a trend toward the increased use of transcriptomics, with studies dominating the literature from 2018 to 2019, and lipidomic applications from 2020 to 2021.
Figure 3.
Status of studies of omics in dolphins.
3. Omics technologies in marine organism: response to contaminants in dolphins
Omic approaches bring an integrated view of the molecules that compose a cell, tissue, or organisms in any target biological sample from a model or non-model organism. Notably, there is little information focused on proteomics, metabolomics, and lipidomics to investigate the impact of contaminants in dolphins species (Figure 2). We present a summary of the application of the three main omic technologies in dolphins associated with contaminants.
3.1 Transcriptomics
Transcriptomics has been the omic technique most used in biological areas because it represents all RNA molecules (e.g., miRNA, snoRNA), including the messenger RNA (mRNA) which constitutes the building blocks for translating DNA into amino acids to form proteins. The totality of mRNA is a reflex of the genes that are actively expressed in a cell or an organism at a given time and during a specific event. It permits deciphering how organisms respond to changes in the external environment or the presence of the contaminants [21]. The principal gene expression profiling methods used in transcriptomic are microarray and RNA-sequencing (RNA-seq). The difference between the potential of each method becomes apparent once the target sequences go beyond known genomic sequences. Hybridization-based techniques like microarray rely on and are limited to the transcripts bound to the array slides. Limitations of microarrays are due to the bioinformatic data available for the model organism’s genome and transcriptome. RNA-seq can detect annotated transcripts but also novel sequences and splice variants [22]. RNA-seq is considered a revolutionary tool for transcriptomics in non-model organisms and is powerful enough to explore the mammalian transcriptome which was not possible with microarrays [23].
With regard to the transcriptomic studies in dolphins and contaminants, there are few studies that have used microarray methods to identify genes and molecular pathways altered by bisphenol A [2,2 bis(4-hydroxyphenyl) propane (BPA), perfluoroalkyl substances (PFAS) and perfluorooctanoic acid (PFOA) in dolphin skin biopsies [15]. These contaminants can cause changes in key genes involved in pathways related to stress, immune response, development and lipid metabolism. Likewise, there are another two studies that describe the construction and validation of the use of microarrays in T. truncatus as well as using bioinformatic tools to detect polychlorinated biphenyls (PCBs) from dolphin blood during the monitoring of high-level contamination at Superfund sites on the Georgia coast in the US [16, 17], see Table 1. A limited range of sequencing data is available for dolphins from whole-genome assemblies to RNA-seq data [18, 19], however, two studies have documented the effects of halogenated organic contaminants (HOCs) at transcriptomic levels. For example, Trego and colleagues reported that 20 skin biopsys from T. truncatus dolphin collected on the Southern California Bight showed to have a positive correlation with the presence of HOCs and genes associated with the metabolism of xenobiotics and with the immune and endocrine pathways. Likewise, in another study also performed with T. truncatus, human peripheral blood mononuclear cells (PBMC) from both species were assessed to investigate the effects of contaminant exposures of CECs (PFAs; PFOA and perfluorooctane sulfonate (PFOS)) using RNA-seq. Transcriptomic analysis showed that in both human and dolphin pathways related with endocrine immune system that inflammatory responses increased (Table 1) [19].
3.2 Proteomics
The main focus of proteomics is to identify and quantify all protein content in a cell, tissue, or organism and understand their functions, structure and their modifications in response to external stimuli [24]. Based on proteomics, baseline studies have been conducted to characterize proteins from spermatozoa and seminal plasma in bottlenose dolphins [25] which has been used in zooarchaeology for species identification of cetaceans [26]. Other studies have been focused on developing bioinformatics tools or methods to obtain or analyze proteins from different samples [27, 28, 29].
Most of the proteomic studies in dolphins have focused on the physiology of proteins and peptides. These studies have provided valuable information, such as the case of the proline-rich antimicrobial peptides found in different cetacean species, where these peptides could provide useful insights for future antibiotics [30]. Through proteomics one can also identify peptides related to metabolic disorders [31] and biomarkers of infection for diagnosis of aspergillosis in dolphins [32]. Thanks to proteomics, it has been possible to identify stress proteins involved in apoptosis, proteotoxicity and inflammation on managed and wild dolphins and their relation with biological data such as serological, biochemical, hematological and endocrine variables [33]. In stressed cetaceans, 30 stress-activated proteins have been identified, where these proteins have an important role in cellular detoxification, stress response, cell growth and differentiation, apoptosis, immunologic, neurologic and hormonal signaling and oxidative stress response [34].
In toxicology, proteomic studies are important because the proteome is the link between effects at the molecular and the whole organism level and provide snapshot functional information of a cell under certain conditions, and it allows the identification of new biomarkers and pathways of toxicity [35]. However, studies related to contamination have not been reported yet.
Regarding the methods and tools used in proteomics, initially, the way to analyze variations of protein expression was by gel electrophoresis. Now the main tool used is mass spectrometry with their different techniques: LC/MS, MALDI TOF/TOF, ESI-QUAD-TOF, iTRAQ. Protein microarray has also been used for these kinds of studies and bioinformatic tools.
Proteomics generates a large amount of data that permit furthering one’s knowledge of mechanisms of action and toxicant effect of a contaminant in organisms and thus be able to understand biological processes [35]. However, the limitations in these kinds of studies are with peptide separations, identification and that many species lack of protein sequence information [14, 36].
3.3 Metabolomics
Metabolomics is responsible for identifying and quantifying all endogenous and exogen metabolites in an organism or biological sample [37]. Metabolites are all final products of cellular processes and knowing their levels permits one to understand the responses of a biological system to environmental changes [38].
This omic tool contributes to understanding of how environmental stressors can affect human and environmental health. However, these kinds of applications have not yet been explored as often in dolphins [39]. Most of the metabolomic studies in dolphins have been focused on establishing baseline information on health [40, 41, 42, 43], and physiology [44, 45, 46] with a few studies looking at the characterization of metabolites from exhaled breath and tears [47, 48].
Regarding pollution studies, just only a single work was discovered. After the spill of the Deepwater Horizon in the Gulf of Mexico, dolphin populations were severely affected, showing adrenal and lung diseases, poor reproductive success and higher mortality [49, 50, 51]. In bottlenose dolphins, Tursiops truncatus exhaled breathe metabolites had been studied [20] from a managed collection in San Diego, from a wild population in Sarasota Bay and Barataria Bay, the latter being the contaminated site. Several metabolites, such as yiamoloside B, diacylglycerol, leptomycin B, phosphatidylglycerol and phospholipids, were correlated with pulmonary disease. Cortisol and aldosterone levels were lower in Barataria Bay, also dolphins from this population presented thin adrenal gland cortices, supporting an impaired hypothalamus-pituitary-adrenal axis. Lower amounts of glucose in the contaminated area may represent a response to stress or feeding. Besides, metabolites as steroids, phosphatidic acid and phosphatidylethanolamine were unique or found in higher abundance in the contaminated area compared to the healthy reference dolphins which suggest cellular destruction. Many of the specific metabolites found in dolphins from Barataria Bay, were markers for arachidonic acid, lipid oxidation and lung surfactant breakdown. In addition, antibiotics, such as jadomycin B, leukomycin A1 and A7, lansonolide A, chivosazole E and mycolacton, were also found in dolphins from Barataria Bay. These compounds are products of fungi and bacteria suggesting that dolphins exposed to oil spill may have pneumonia.
In metabolomics, the main tools used for analysis are mass spectrometry with their different instrumentation: chromatography/mass spectrometry (GC/MS), liquid chromatography/mass spectrometry (LC/MS) and in tandem (LC/MS/MS), high-performance liquid chromatography (HPLC), HPLC-MS/MS, reverse phase chromatography (RP)/UPLC-MS/MS, capillary electrophoresis time of flight mass spectrometer (CE-TOFMS), liquid chromatography/time-of-flight/mass spectrometry (LC-TOFMS) and the least used are nuclear resonance magnetic (NMR) and high-resolution magic angle spinning (HR-MAS) NMR spectroscopy.
Metabolomics is relatively a new tool and captures more integrated information of the physiology of an organism than transcriptomics or proteomics [52] because it represents the final cellular signaling events, resulting from transcriptional and translational changes [39]. However, it presents some limitations such as targeting metabolites that are species specific as well as libraries and software programs that are not yet sufficiently extensive [52].
3.3.1 Lipidomics
Lipidomics is a specialized subfield of metabolomics. Through lipidomics, it is possible to characterize all lipids from a cell, tissue, fluid, etc. and understand how these lipids influence a biological system and participate in several processes as well as how they interact with other molecules and respond to environmental changes [53, 54]. Lipids represent a major component of the metabolome [54], have an important role as components of cell membranes and participate in many cellular pathways and due to these being involved in many physiological mechanisms, also are excellent candidates for monitoring the effects of stress [55].
One representative area in marine mammals is their blubber. This is the most important site of fat and energy storage and also participates in different processes such as insulation, thermoregulation and buoyancy and, it represents up to 50% of the body mass [56] and due to the great quantity of lipids, it makes it a good repository for contaminants that are lipophilic [57]. For these reasons, lipidomics makes an excellent tool for studying the effects of contamination in these sentinel species. Although lipidomic studies have been increasing in recent years, until now, there are no dolphin lipidomic studies related to contamination. Indirectly, one study focused on respiratory metabolites [20], where some lipids were detected, including phosphatidylethanolamine, from oil spill exposure. These lipids were found in higher concentrations in dolphins from the contaminated area.
Few lipidomic studies have been reported, with most focused on physiology [58, 59], and characterization of lipids from cardiac phospholipidome [59] of small cetaceans and lipids from the blubber of killer whales [60].
The main tool used for lipidomic studies is mass spectrometry. This analysis generally uses another instrument such as LC-electrospray ionization (ESI) quadrupole time-of-flight (Q-TOF), liquid chromatography-high-resolution mass spectrometry (LC/HRMS/MS), GC-MS and LC-MS/MS and hydrophilic interaction liquid chromatography-mass spectrometry (HILIC-LC-MS).
4. Omics and dolphin: future considerations
This review integrates the available information on the effects of pollution using an omics approach on dolphins and other cetaceans considered as ideal organisms to assess and monitor pollution in coastal or ocean systems. Although there are wide applications of omic approaches in other model and non-model aquatic organisms involving environmental matters, there are very few studies from an omic perspective in dolphins. There is much evidence in the literature of the analytical power that these tools have their contribution in providing relevant information on the MOA of contaminants in cells, tissues, organisms or populations to help to assess the health status of marine systems, to identify potential biomarkers of exposure and response to the contaminant as well to predict adverse effects on marine organisms. Information provided from this study may be useful for risk assesment analysis that may impact future environmental regulations. However, there are still several limitations that need addressing in their application in dolphins. (1) One of the main challenges is with sampling (non-invasive/biopsy). There are prohibitive costs and time delays associated with obtaining the permits required to obtain samples in wildlife organisms in some countries. A non-ideal but possible option is the sampling of strandings. (2) The application of omic studies in ecotoxicology still has many challenges. The increase of these studies at different omic levels has grown impressively thus requiring improved bioinformatics and computational tools for better analysis regarding environmental stressors, such as pollutants. (3) Likewise, the collaboration between academic government entities and industry still needs to be improved.
5. Conclusions
This review highlights the importance of omic studies in dolphins which have contributed greatly in recognizing the presence and effect of contaminants such as HOC, CECs (BPA and PFOs) and those associated with oil spills (summarized in Table 1). Omics technologies are important to study adverse effects of contaminants or environmental changes because they provide information on the alterations of genes, proteins, metabolites and phenotypic responses [14]. Transcriptomic-based investigations were used most frequently (31%); only a few studies used a metabolomic approach (8%). The principal tool used for transcriptomic is RNA-seq and for proteomics, metabolomics and lipidomics is mass spectrometry coupled to different types of spectrometers (Figure 1).
Some of the more likely applications for omics in dolphins are characterization and physiology. Although omics studies have been used for many topics, the number of studies concerning contamination is rather low. Studies of proteomics, metabolomic and lipidomic are still lacking; therefore, these findings may give insight for future studies. This type of study contributes greatly in establishing baselines for environmental health studies of coastal and marine systems, the health status of the dolphin reflects the status of their environment. Perhaps it may allow the local as well as the scientific community to be more aware of marine ecosystem conditions and to recognize the importance and possibilities of integrate omics studies regarding pollution.
Conflict of interest
The authors declare no conflict of interest.
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Establishment of epidermal cell lines derived from the skin of the Atlantic bottlenose dolphin (Tursiops truncatus). The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology. 2005;287A:1246-1255'},{id:"B28",body:'Dooley CT, Ferrer T, Pagán H, O’Corry-Crowe GM. Bridging immunogenetics and immunoproteomics: Model positional scanning library analysis for major histocompatibility complex class II DQ in Tursiops truncatus. PLoS One. 2018;13(8):e0201299'},{id:"B29",body:'Bergfelt DR, Lippolis J, Vandenplas M, Davis S, Miller BA, Madan R, et al. Preliminary analysis of the proteome of exhaled breath condensate in bottlenose dolphins (Tursiops truncatus). Aquatic Mammals. 2018;44(3):256-266'},{id:"B30",body:'Sola R, Mardirossian M, Beckert B, De Luna LS, Prickett D, Tossi A, et al. Characterization of cetacean proline-rich antimicrobial peptides displaying activity against eskape pathogens. 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Molecular analysis of stress-activated proteins and genes in dolphins and whales: A new technique for monitoring environmental stress. In: Annual Conference-American Association of Zoo Veterinarians. Vol. 2000. Houston, TX: American Association of Zoo Veterinarians; 1998. pp. 240-244'},{id:"B35",body:'Lemos MFL, Soares AMVM, Correia AC, Esteves AC. Proteins in ecotoxicology—How, why and why not? Proteomics. 2010;10:873-887'},{id:"B36",body:'Monsinjon T, Knigge T. Proteomic applications in ecotoxicology. Proteomics. 2007;7:2997-3009'},{id:"B37",body:'Idle JR, Gonzalez FJ. Metabolomics. Cell Metabolism. 2007;6:348-351'},{id:"B38",body:'Fiehn O. Metabolomics—The link between genotypes and phenotypes. Plant Molecular Biology. 2002;48:155-171'},{id:"B39",body:'Miller MG. Environmental metabolomics: A SWOT analysis (strengths, weaknesses, opportunities, and threats). Journal of Proteome Research. 2007;6:540-545'},{id:"B40",body:'Venn-Watson S, Baird M, Novick B, Parry C, Jensen ED. Modified fish diet shifted serum metabolome and alleviated chronic anemia in bottlenose dolphins (Tursiops truncatus): Potential role of odd-chain saturated fatty acids. PLoS One. 2020;15(4):1-23'},{id:"B41",body:'Ardente A, Garrett T, Wells R, Walsh M, Smith C, Colee J, et al. A targeted metabolomics assay to measure eight purines in the diet of common bottlenose dolphins, Tursiops truncates. Journal of Chromatography and Separation Techniques. 2016;7(5):1-22'},{id:"B42",body:'Borras E, Aksenov AA, Baird M, Novick B, Schivo M, Zamuruyev KO, et al. Exhaled breath condensate methods adapted from human studies using longitudinal metabolomics for predicting early health alterations in dolphins. Analytical and Bioanalytical Chemistry. 2017;409:6523-6536'},{id:"B43",body:'Houser DS, Derous D, Douglas A, Lusseau D. Metabolic response of dolphins to short-term fasting reveals physiological changes that differ from the traditional fasting model. The Journal of Experimental Biology. 2021;224:1-12'},{id:"B44",body:'Suzuki M, Yoshioka M, Ohno Y, Akune Y. Plasma metabolomic analysis in mature female common bottlenose dolphins: Profiling the characteristics of metabolites after overnight fasting by comparison with data in beagle dogs. Scientific Reports. 2018;8:1-11'},{id:"B45",body:'Misra BB, Ruiz-Hernández IM, Hernández-Bolio GI, Hernández-Núñez E, Díaz-Gamboa R, Colli-Dula RC. 1H NMR metabolomic analysis of skin and blubber of bottlenose dolphins reveal a functional metabolic dichotomy. Comparative Biochemistry and Physiology Part D: Genomics and Proteomics. 2019;30:25-32. DOI: 10.1016/j.cbd.2019.02.004'},{id:"B46",body:'Velasco-Martínez I del C, Hernández-Camacho CJ, Méndez-Rodríguez LC, Zenteno-Savín T. Purine metabolism in response to hypoxic conditions associated with breath-hold diving and exercise in erythrocytes and plasma from bottlenose dolphins (Tursiops truncatus). 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New York, NY: Humana Press; 2017. p. 248'},{id:"B55",body:'Aristizabal-Henao JJ, Ahmadireskety A, Griffin EK, Ferreira Da Silva B, Bowden JA. Lipidomics and environmental toxicology: Recent trends. Current Opinion in Environmental Science & Health. 2020;15:26-31. DOI: 10.1016/j.coesh.2020.04.004'},{id:"B56",body:'Iverson SJ. Blubber. In: Perrin WF, Würsig B, JGM T, editors. Encyclopedia of Marine Mammals. 2nd ed. Academic Press; 2009. pp. 115-120. DOI: 10.1016/B978-0-12-383832-2.00112-2'},{id:"B57",body:'Reddy ML, Dierauf LA, Gulland FMD. Marine mammals as sentinels of ocean health. In: Dierauf LA, Gulland FMD, editors. Handbook of Marine Mammal Medicine. 2nd ed. Boca Ratón, FL: CRC Press; 2001. pp. 3-13'},{id:"B58",body:'Sobolesky PM, Harrell TS, Parry C, Venn-Watson S, Janech MG. Feeding a modified fish diet to bottlenose dolphins leads to an increase in serum adiponectin and sphingolipids. 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CONACYT, México
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Departamento de Recursos del Mar, Centro de Investigación y de Estudios Avanzados del IPN, México
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