\r\n\t* Technologies that allow control and reduction of air pollutants are encouraged to be discussed. In particular, technologies employed to treat greenhouse gases and precursors of acid rain. \r\n\t* Poor agricultural practices, improper waste management and extractive activities that contaminate soil with special attention focused on emerging techniques permitting to diminish these pollutants. \r\n\t* In the treatment of freshwater and marine and coastal waters, technologies that should be taken into account are those focused to eliminate chemicals and pathogens from mining and industrial effluents. \r\n\t* Renewable energy technologies should also be discussed, special interest in those having the lower environmental impact. In this case a watchful life-cycle analysis has to support the proposal. \r\n\t* New technologies and materials allowing the energy storage in a competitive mode should be also taken into account for the reason that they have a direct impact in the decrease of pollutants. \r\n\t* As a final point, technological innovations applied to conserve and study endangered species will be also considered.
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1. Introduction
Ischemic heart disease is a clinical syndrome resulting from myocardial ischemia and is characterized by an imbalance between the supply and demand of myocardial blood flow and myocardial oxygen metabolism. It is currently one of the major diseases that endanger human health. Early and effective reconstruction of ischemic myocardial blood perfusion is the fundamental measure taken to prevent the development of ischemic myocardial injury, reduce myocardial infarct size, and improve the clinical prognosis. However, several studies have discovered that in some cases, reperfusion of ischemic cells could cause further injury in the form of ischemia/reperfusion injury. The clinical manifestations of myocardial ischemia-reperfusion injury include arrhythmia, myocardial stunning, and no-reflow. Although lethal reperfusion injury in clinical practice is more difficult to identify, it is the most serious consequence of ischemia/reperfusion injury and is also the main reason preventing the ischemic myocardium recovery from effective reperfusion therapy. Therefore, studies on the modes of myocardial cell death after ischemia/reperfusion are of great significance. Previous studies suggested that myocardial cell death following myocardial ischemia/reperfusion injury were mainly necrosis and apoptosis. Apoptosis receives more attention due to its death program. However, in recent years, a number of studies have suggested that, another procedural manner of death---autophagy, type II programmed cell death, also plays a critical role in ischemia/reperfusion injury. The study of this death pathway may provide a new effective way to block myocardial ischemia/reperfusion injury. Therefore, in this chapter, the roles and possible mechanisms of autophagy in myocardial ischemia/reperfusion injury will be reviewed.
2. Definition, formation and classification of autophagy
In 1962, Ashford and Porter discovered the ‘self-eating’ phenomenon in liver cells using an electron microscope [1]. After that, this process was named autophagy by Duve, a Belgian cellular biologist and chemist [2].
Autophagy is the transportation and degradation of damaged, denatured or aged proteins. It is a common cellular physiological process which can maintain cell homeostasis. Autophagy is an evolutionary conserved pathway of self-digestion that occurs in various eukaryotic organisms from yeast to mammals [3]. It is also a cellular defense mechanism in many pathological processes. The autophagy process time is relatively short (T1/2 for 8 mins), it illustrates that autophagy is an effective response to environmental changes for cells, and it plays a pivotal role in metabolism: (1) Since autophagy is an adaptive response to exogenous stimuli (including nutritional deficiencies, the cell density load, hypoxia, oxidative stress, infection, etc.), and its products of degradation, such as amino acids, nucleotides, and free fatty acids, can participate in the material and energy cycle; (2) As a housekeeper mechanism for cells to maintain a steady state, autophagy can adjust the renovation of long-lived proteins, peroxisomes, mitochondria and endoplasmic reticulum; (3) Autophagy is involved in tissue-specific integration; (4) Autophagy can act as a defense mechanism to remove the damaged cytoplasm and metabolites, and the reconstruction on the subcellular level can protect the affected cells. Meanwhile, as a cell death procedure, autophagy can induce cell initiative death [4].
Although autophagy and autophagy-related processes are dynamic, they can be broken down into several discrete steps. There are four steps in autophagy: induction, formation of autophagosomes, formation of autolysosomes and degradation of the content. Autophagy serves as a response to stress such as nutrient limitation, and this is one of its primary roles in unicellular organisms such as yeast. Then, portions of cytoplasm are first isolated (sequestered) within a double membrane enclosed vacuole called an autophagosome. In studies on yeast, the isolation membrane has been shown to develop from a small vesicle that later transforms into a cup-like structure surrounding the material to be degraded [5]. The formation of an autophagosome (sequestration) is complete when the edges of the ‘cup’ merge. It has been found that the proteins forming the isolation membrane in yeast are unique, different from those in other cellular compartments, which suggests a de novo formation of this membrane [6]. It is still discussed whether the isolation membrane also forms de novo in higher organisms, including mammals, or if it originates from another organelle, such as endoplasmic reticulum, lysosome or Golgi complex. The sequestration membrane that later gives rise to the autophagosome has been termed phagophore or pre-autophagosome [7]. In yeast and mammalian cells, autophagy occurs at a basal level. The general diameter of autophagosomes is 300-900 nm, and the average level is 500 nm. Since the beginning of the formation of the autophagosomes, cytoplasm and nucleoplasm become darker, but the nucleus structure has no noticeable changes. Mitochondria and endoplasmic reticulum swell, Gorky body expands, and then the membrane-specialized structures such as microvilli disappear, membrane foamed and retracted. At the later stages of autophagy, the volume and number of autophagosomes filled with myelin or liquid increase, and then some gray ingredients and a small number of condensated nuclear materials will exist. These features can be used as morphological indicators during inspection.
Depending on the different ways cellular material is transported to lysosomes, there are three types of autophagy: microautophagy, macroautophagy, chaperone-mediated autophagy (CMA). Macroautophagy is commonly referred to as autophagy, and the cytoplasm is wrapped by the dropped bilayers from the non-ribosomal region of endoplasmic reticulum, the Golgi apparatus and other bilayers. In microautophagy, it also goes through the same process of wrapping, but the substrate is engulfed by inward invagination of the lysosomal membrane. In the process of CMA, intracytoplasmic proteins are bound to the molecular chaperone, then transported to the lysosome cavity, and digested by the lysosomal enzyme. CMA sequester proteins that expose a KFERQ-like pentapeptide which are mediated by heat shock cognate 70 (HSC70) and its co-chaperones. Lysosomal-associated membrane protein 2 (LAMP-2A) acts as a receptor on the lysosome and mediates the degradation of unfolded proteins. Macroautophagy is the major regulated cellular pathway for degrading long-lived proteins and the only known pathway for degrading cytoplasmic organelles (Figure 1). Therefore, this chapter focuses on macroautophagy.
3. Signaling regulation of autophagy
Autophagy is highly conservative during the evolution process. Homologous gene participation in autophagy not only be found in yeasts and Drosophila melanogaster but also in vertebrates and humans. In order to unify the standard, Klionsky, in 2003, named these homologous genes as autophagy-related genes (Atg) to stand for autophagy genes and the corresponding proteins [8]. So far, scientists have already found more than 30 Atgs and most of their homologous analogues. In addition, the Atg protein can be divided into five groups: Atg1 protein kinase complex, Atg9, the class III phosphatidylinositol 3-kinase (PI3K)-Beclin1complex, Atg12 conjugation system and Atg8 conjugation system. Atg1 protein kinase complex is essential for the induction of autophagy. There are two mammalian homologs of Atg1 that appear to function in autophagy, the Unc-51-like kinase 1 (ULK1) and -2 (ULK2), which is the mammalian homologues of serine/threonine protein kinase Atg1, existing in the form of ULK1-mammalian Atg13 (mAtg13) -focal adhesion kinase family interacting protein of 200 kDa (FIP200) -Atg10 complex [9]. Under nutritional deficiency conditions, ULK1 is activated and then phosphorylates mAtg13, FIP200 and itself to initiate autophagy. Regarding the substrates of the Atg1 kinase during autophagy, it is suggested that mAtg13 and FIP200 are phosphorylated by ULKs, and ULKs also undergo autophosphorylation, which is conducive to a conformational change and autophagy induction. Mammalian target of rapammycin (mTOR) can phosphorylate and inactivate ULKs and Atg13 under nutrient-rich conditions. Upon mTOR inhibition by starvation or rapamycin, ULK1 and ULK2 are activated and phosphorylate Atg13 and FIP200, which are essential for autophagy activity. Recent studies have suggested that Atg13 may be phosphorylated by TOR or Atg1/ULKs on different residues [10]. It is likely that phosphorylation of Atg13 is dependent to a greater extent on TOR in yeast, but on Atg1 in Drosophila.
Figure 1.
Different Types of Autophagy (Cite from < Mizushima N, Komatsu M. Cell 2011;147(4) 728-741>).
The conjugation of Atg12 and Atg8 is essential for the formation of autophagosomes. Atg12 was the first ubiquitin-like Atg protein to be identified, which can be activated by Atg7 and Atg10. Then it is conjugated to Atg5 and promotes the formation of the autophagy precursor [11]. The amino-acid sequence of Atg12 ends with a glycine residue and there is no protease involved in Atg12 conjugation. Analogous to ubiquitination, there is an E1-like enzyme, Atg7, and Atg12 is activated by forming a thioester bond between the C-terminal Gly 186 of Atg12 and the Cys 507 of Atg7. After activation, Atg12 is transferred to Atg10, which is an E2 enzyme, and is eventually conjugated to the target protein Atg5 at Lys 149 through an isopeptide bond. There is no typical E3 enzyme involved in Atg12-Atg5 conjugation. Atg5 interacts further with a small coiled-coil protein, Atg16, and Atg12-Atg5-Atg16 forms a multimeric complex through the homo-oligomerization of Atg16. The ubiquitin-like (Ubl) protein Atg8 is attached to phosphatidylethanolamine (PE). The C-terminal Arg 117 residue of Atg8 is initially proteolytically removed by a cysteine protease, Atg4, to expose Gly 116. This exposed glycine forms a thioester bond with Cys 507 of Atg7, which is also the site that participates in the Atg12-Atg5 conjugation. This feature differentiates Atg7 from most other E1 enzymes, which activate single Ubl proteins. Activated Atg8 is then transferred to the E2-like enzyme Atg3, also through a thioester bond. In the final step of Atg8 lipidation, Gly 116 of Atg8 is conjugated to PE through an amide bond; Atg8-PE exists in a tightly membrane-associated form. Microtubule-associated protein l light chain 3 (LC3) is the mammalian homologues of Atg8. LC3 is activated by Atg7, transferred to Atg3, and conjugated to phosphatidylethanolamine (PE) on the surface of autophagic vacuoles membranes to promote the forming of autophagosomes [12].
Recently, the PI3K-Becline 1 complex and Atg9 have been shown to be the essential components involved in autophagy signaling and the membrane transportation of autophagic vacuoles. This autophagy-specific class III-PI3K-Becline 1 complex appears to be essential to recruit the Atg12-Atg5 conjugation to the pre-autophagosomal structure. Atg12-Atg5 conjugation is then required for the elongation of the isolation membrane and for the proper localization of conjugated LC3/Atg8 [13]. Atg9 is required for autophagy in both yeast and mammalian cells and has been speculated to be involved in delivery of membrane lipids to form autophagosomes. In mammalian cells, mAtg9 traffics between the trans-Golgi network, endosomes and newly formed autophagosomes [14].
Cells regulate autophagy through a set of precise signaling pathways including integrating nutriment, growth factors, hormones, stress and intracellular energy information. A key regulator point of autophagy in mammals is kinase mTOR. The kinase TOR is a major evolutionarily conserved sensor in the autophagy signaling pathway in eukaryotes, but it also regulates many other aspects of cell function, including transcription, translation, and cell size and cytoskeletal organization. In mammals, mTOR can be included in two different complexes, mTORC1 and mTORC2. Although these two TOR complexes share common components, they display distinct cellular functions and phosphorylate different downstream substrates. The activity of mTORC1 is regulated via the integration of many signals, including growth factors, insulin, nutrients, energy availability, and cell stressors such as hypoxia, osmotic stress, reactive oxygen species (ROS) and viral infection. mTORC1 is the only known target of the drug rapamycin and is required for signaling to ribosomal S6 kinases (S6K) and eIF4E-binding proteins (4EBP1 and 4EBP2). mTORC1 has recently been shown to consist of four proteins: mTOR, mLST8 (also known as GbL), proline-rich PKB/Akt substrate 40-kDa (PRAS40), and raptor (regulatory associated protein of mTOR); and it plays a major role in controlling translation and cell growth in response to nutrients. The adaptor protein is common to both mTOR complexes. Raptor binds mTOR, S6K and 4EBP1/2 and facilitates mTOR phosphorylation of these molecules; but whether raptor enhances or represses mTOR kinase activity remains unclear [15]. Unlike mTORC1, mTORC2 has some functions that cannot be inhibited by rapamycin, including the control of actin cytoskeleton dynamics. The mTORC2 complex consists of mTOR, mLST8, mammalian stress-activated protein kinase-interacting protein 1 (mSin1), and rapamycin-insensitive companion of mTOR (rictor). Recent studies indicate that when eutrophy, mTOR activates the PI3K-I/ protein kinase B (PKB) signaling pathway, it leads multiple serine sites to phosphorylation and then reduces the affinity of Atg13 and Atg1. Since Atg1-Atg13 compounds decreased, Atg9 cannot be transferred to the autophagosome formation sites and autophagy was inhibited.
AMP-activated protein kinase (AMPK) was initially identified as a serine/threonine kinase that negatively regulates several key enzymes of the lipid anabolism. Meanwhile, AMPK is regarded as the major energy-sensing kinase that activates a whole variety of catabolic processes in multicellular organisms such as glucose uptake and metabolism, while simultaneously inhibiting several anabolic pathways, such as lipid, protein, and carbohydrate biosynthesis. Activated AMPK can inhibit mTOR by interfering with the activity of GTPase Rheb and with protein synthesis, degrade the phosphorylation of ULK1 and promote disintegration of ULK1 from the mTOR compounds. In starved cells, when the AMP/ATP ratio increases; the binding of AMP to AMPK promotes its activation by the AMPK kinase LKB. Moreover, Ca2+/calmodulin-dependent kinase kinase beta (CaMKK-beta) has been identified as being an AMPK kinase. The activity of AMPK is required for autophagy to be induced in response to starvation in mammalian cells and in yeast in a TORC1-dependent manner. Moreover, autophagy induction is also dependent on the inhibition of mTORC1 by AMPK in non-starved cells in response to an increase in free cytosolic Ca2+. In this setting, the activation of AMPK and stimulation of autophagy are dependent on CaMKK-beta. The induction of autophagy through AMPK activation probably also occurs in other settings, such as hypoxia. AMPK is probably a general regulator of autophagy upstream of mTOR [16]. Another potential candidate of autophagy regulation down-stream of AMPK is elongation factor-2 kinase (eEF-2 kinase), which controls the rate of peptide elongation [17]. Activation of eEF-2 kinase increases autophagy and slows protein translation. The activity of eEF-2 kinase is regulated by mTOR, S6K, and AMPK. During periods of ATP depletion, AMPK is activated and eEF-2 kinase is phosphorylated, leading to a balance between the inhibition of peptide elongation and the induction of autophagy. However, how eEF-2 kinase impinges on the molecular machinery of autophagy remains to be elucidated.
There also is an indirectly-TOR-dependent signaling pathway, named class I PI3K/Akt pathway, which when responding to insulin-like and other growth factor signals, the signaling molecules link receptor tyrosine kinases to activate TOR kinase and thereby repress autophagy. In addition, autophagy can mediate inactive proteins to degrade to amino acids and then provide metabolic substrates for cardiac development and ischemic hypoxia forming a cardiac protection method. Autophagy is among the important mechanisms of hypoxic adaptation and is perhaps one of the last resorts for the salvage of ATP in hypoxic cells and organs.
p53 is a responsive stress protein which also plays a crucial role in the regulation of autophagy through the transcription-dependent/independent pathways. In the transcription-independent pathway, p53 can activate AMPK and down-regulate mTOR; in the transcription-dependent pathway, through the up-regulation of PTEN (inhibitor of mTOR), the tuberous sclerosis-1 (TSC1) gene or cell death gene damage-regulated autophagy modulator 1 (DRAM1), mTOR is down-regulated, and autophagy is induced (Figure 2). In addition, c-jun N signal kinases (JNK), GTPases, Erk1/2, ceramide are also involved in regulating autophagy. The cytoplasmic form of p53 has been shown to have an inhibitory effect on autophagy, suggesting that activation of autophagy by p53 depends on its transactivating effect on genes such as DRAM1 [18].
Figure 2.
Signaling Pathway of Autophagy (Cite from http://www.cellsignal.com/).
4. The role of autophagy in the maintenance of normal myocardium
In the basal state, autophagy showed low expression in the heart to maintain normal myocardial function. And most of its function is to perform homeostatic functions by eliminating long-live organelles and proteins. Autophagy can be upregulated rapidly when myocardium cells need to generate intracellular nutrients and energy, for example during starvation or trophic factor withdrawal. Nutritional status, hormonal factors, and other cues like temperature, oxygen concentrations, and cell density are important in the control of autophagy.
The molecular mechanism of autophagy is still poorly understood. There are more than 30 genes that have been confirmed to be related to autophagy, and half of them are highly conservative in most metazoa [19]. Knockout Atg5 gene (a decisive gene in autophagy) in early stage adult rat hearts has showed no obvious abnormality, however, after increased afterload for one week, left ventricular can cause myocardial ubiquitination and mitochondrial aggregation, resulting in myocardial hypertrophy and decrease in myocardial contractility, which indicates that autophagy may play an important role in maintenance of homeostasis, size of myocardium, general construction and function of myocardial cells. Paradoxically, partially reduced autophagic activity caused by heterozygous deletion of Beclin 1 improves cardiac function upon pressure overload. Consistent with this phenomenon, partial suppression of autophagy with histone deacetylase (HDAC) inhibitors can ameliorate pressure overload-induced cardiac hypertrophy in mice [20]. These data suggest that partial, but not complete, suppression of autophagy may be beneficial.
In short, autophagy adapts to the myocardial energy demand by maintaining energy metabolism, so as to protect the myocardial function.
5. The protective effect of autophagy in myocardial ischemia/hypoxia
In recent years, a large number of studies suggested that autophagy played a protective role to rescue cardiomyocytes in ischemia/hypoxia. In [21], researchers discovered that 40 mins of hypoxia induced significant autophagosome and autolysosome formation, according to a rabbit hypoxic heart model. At the same time, ultrastructural analysis revealed autophagosomes in close proximity to swollen and fragmented mitochondria. And in rodents, 30 mins after ischemia induced dramatic up-regulation of autophagosome formation [22]. Previous report also demonstrated that the level of autophagy was rapidly increased within 30 mins after coronary ligation in mice, especially in the risk area (salvaged cardiomyocytes bordering the infarcted area) [23]. Recent work has revealed that inactivation of hypoxia-inducible factor 1α (HIF-1α) in fibroblasts blunts hypoxia-induced autophagy [24]. These results strongly suggested that autophagy was activated by myocardial ischemia/hypoxia. Furthermore, these investigators reported that suppression of autophagy using 3-methyladenine or bafilomycin A1 enhanced myocyte death triggered by glucose deprivation. During postinfarction cardiac remodeling, lysosomes and autophagosomes became more numerous in the cardiomyocytes, thus ensuring that autophagy could provide enough energy to cardiomyocytes. The protective effects of autophagy may be that cells were likely to be provided energy, free amino acids and fatty acids through decomposition of their own material.
Additionally, autophagy may maintain cardiomyocyte survival after ischemia/hypoxia by inhibiting apoptosis [25]. Autophagy is a recycling process of cytoplasmic components, such as long-lived proteins and organelles. The prosurvival role of autophagy has been observed in yeast, plants, flies, and mammals. Inhibition of autophagy results in accumulation of cytoplasmic components and promotion of apoptosis. Treatment with pharmacological autophagy inhibitors and knockdown of Atg genes (Atg5, Atg10, Atg12, and Beclin 1) can increase apoptosis and cell death in nutrient-deprived cells. In reference [26], autophagy delayed apoptotic cell death in breast cancer cells following DNA damage. These results suggested that the effect of inhibiting apoptosis may be related to autophagosomes wrapping damaged mitochondria, because it will not only prevent the release of cytochrome C, but also inhibit the formation of apoptotic bodies.
Numerous studies show that mTOR is a key factor in regulating autophagy in ischemia/hypoxia. In mammalian cells, phosphorylation of mTOR inhibits cell autophagy. Conversely, dephosphorylation of mTOR enhances autophagy. The activity of mTOR is adjusted by many factors. AMPK is the important factor which would inhibit the activity of mTOR to enhance autophagy in ischemia. AMPK serves as a general integrator of metabolic responses to changes in energy availability and is activated in response to elevations of the AMP/ATP ratio. Data in reference [27] suggested that autophagy has been reported to be up-regulated in response to reduced cellular content of ATP. In cultured cardiac myocytes, glucose deprivation caused significant reduction in the levels of ATP, which coincided with up-regulation of autophagy. Moreover, myocardial ischemia causes a decrease in ATP levels and an increase in the AMP/ATP ratio, resulting in activation of the AMPK [28]. Under conditions of stress (including hypoxia and ischemia), a signaling cascade is initiated involving phosphorylation of AMPK and subsequent inhibition of mTOR. Inhibition of mTOR, in concert with other protein partners, provides the critical step in initiating autophagosome formation.
6. The expression and regulation of autophagy during myocardial ischemia/reperfusion
Autophagy is induced during myocardial ischemia. Although it was speculated that activation of autophagy may be reverted when ischemia is relieved, in fact, autophagy may be further enhanced by reperfusion [22, 29]. A variety of factors that can regulate the autophagy during myocardial ischemia/reperfusion, such as ROS generated by mitochondrial respiration, endoplasmic reticulum stress, calcium, vitamin D compounds, ATP, thapsigargin, calcium protease, and so on. Different signal transduction pathways are involved in the occurrence of autophagy at different stages of myocardial ischemia/reperfusion.
6.1. The role of Beclin 1 in the occurrence of autophagy during myocardial ischemia/reperfusion
Induction of autophagy in the ischemic phase was accompanied by activation of AMPK and mTOR. In contrast, autophagy during reperfusion was accompanied by upregulation of Beclin 1 rather than by activation of AMPK. Induction of autophagy and cardiac injury during the reperfusion phase was significantly attenuated in Beclin 1+/- mice. Collectively, in the ischemic heart, autophagy is stimulated through an AMPK-dependent mechanism, whereas ischemia/reperfusion stimulates autophagy through a Beclin 1-dependent but not an AMPK-independent pathway [22]. Using cultured cardiomyocytes, previous studies have demonstrated that the inhibition of autophagy by urocortin during the reperfusion phase is mediated in part by inhibition of Beclin 1 expression, an effect which is mediated by activation of the PI3K/Akt pathway [30]. Recent experimental data also show that the clearance of autophagosomes is impaired in myocardial reperfusion injury which is mediated in part by ROS-induced decline in LAMP-2A and upregulation of Beclin 1, contributing to increased cardiomyocyte death [31, 32]. Thus, ROS and ROS-mediated upregulation of Beclin 1 in the myocardial reperfusion phase may play an important role in the occurrence of autophagy. Therefore, it is now widely recognized that the autophagy was induced through the AMPK-eEF2K/mTOR pathway during the ischemic phase of ischemic/reperfusion injury, but was triggered through the Class III PI3K/Beclin 1 pathway during the reperfusion phase.
6.2. The role of Bcl-2 family members in the occurrence of autophagy during myocardial ischemia/reperfusion
It is well known that the Bcl-2 family proteins play essential roles in regulating apoptosis in the cardiovascular system, and several studies have revealed that the Bcl-2 family members (Bnip3, Bcl-2, Bcl-XL, Bax, etc.) also play important roles in the induction of autophagy during myocardial ischemia/reperfusion injury.
6.2.1. Bnip3
Bnip3 (Bcl-2/adenovirus E1B-19 KD interacting protein 3) with a single Bcl-2 homology 3 (BH3) domain is a pro-apoptotic Bcl-2 family protein which is most sensitive to hypoxia and plays an important role in myocardial ischemia/reperfusion injury. It was found that the overexpression of Bnip3 significantly increased autophagy, whereas, overexpression of the dominant-negative Bnip3 significantly reduced autophagy induced by myocardial ischemia/reperfusion in HL-1 cardiac myocytes [33]. These results suggest that Bnip3 plays a fundamental role in the induction of autophagy during myocardial ischemia and reperfusion. However, more studies are still needed to clarify the role of Bnip3 in response to ischemia/reperfusion, and the most reasonable explanation is that the mitochondrial dysfunction caused by Bnip3 can enhance the level of autophagy in order to remove damaged organelles.
6.2.2. Bcl-2/Bcl-XL and Bax
Bcl-2 family members are key modulators of apoptosis that have recently been shown to also regulate autophagy. Transgenic mice overexpressing the anti-apoptotic human Bcl-2 cDNA in the heart is effective at reducing myocardial reperfusion injury and improving heart function [34, 35]. However, blockage of the activity of the proapoptotic molecule Bax in a knockout mouse model attenuates ischemia/reperfusion injury [36]. Recent reports demonstrated that Beclin 1 possessed a BH3 domain. The BH3 domain of Beclin 1 is bound to and inhibited by Bcl-2 or Bcl-XL [37, 38]. A BH3 mutant of Beclin1 which has reduced affinity for Bcl-XL/Bcl-2 was a more potent inducer of autophagy than wild type Beclin 1. Overexpression of Bcl-2 in the heart reduced starvation-induced autophagy. Thus, Bcl-2 not only functions as an antiapoptotic protein, but also as an antiautophagy protein via its inhibitory interaction with Beclin 1. These antiapoptosis and antiautophagy functions of Bcl-2 may protect the myocardium against ischemia/reperfusion injury [39]. Meanwhile, some studies found that Bnip3 enhanced autophagy, possibly due to competitive disruption of Bcl-2 binding to Beclin 1 [24] or interacting with Rheb to inhibit mTOR [40]. Of course, more studies are needed to clarify these relationships.
6.3. The role of angiotensin II (Ang II) receptor signaling in the induction of autophagy during myocardial ischemia/reperfusion
Recent report demonstrated that overexpression of Ang II type 1 (AT1) receptor caused a significant increase in autophagy after treatment with Ang II in cultured neonatal rat cardiomyocytes. However, overexpression of the Ang II type 2 (AT2) receptor can inhibit autophagy in an Ang II-independent manner. Neonatal cardiomyocytes cultured from hypertrophic heart rats (HHRs) were more susceptible to AT1 receptor-stimulated autophagy than cardiomyocytes from normal heart rats (NHRs). Moreover, there was a greater up-regulation of autophagic markers in adult HHR hearts than in NHR hearts following ischemia/reperfusion in vitro [41]. Additionally, AT1 receptor blockaded with olmesartan plays a protective role in myocardial ischemia-reperfusion injury [42]. Therefore, it is inferred that Ang II/AT1 receptor signaling might also be involved in the stimulation of autophagy during myocardial ischemia/reperfusion.
Thus, in addition to the Beclin 1, many Bcl-2 family members and angiotensin II/AT1 receptor signaling may also be involved in the stimulation of autophagy, but additional studies are needed to clarify the role of these pathways in the occurrence of autophagy during ischemia/reperfusion injury of the heart.
7. The role of autophagy in myocardial ischemia/reperfusion
The autophagy is induced during the ischemia/reperfusion process; however, the role of autophagy during myocardial ischemia/reperfusion is still inconclusive.
It is well-documented that autophagy occurs at basal levels but can be further induced by stresses, such as nutrient depletion. Autolysosomal degradation of membrane lipids and proteins generate free fatty acids and amino acids, which can be reused to maintain mitochondrial ATP production and protein synthesis and promote cell survival. When the myocardium was suppressed with ischemia, the blood supply was decreased and the energy was insufficient, which means that there was an inadequate supply of nutrients. In these conditions, AMPK acts as a sensor for energy deprivation and activation of AMPK mediates metabolic adaptation during ischemia.
Many studies have shown that the autophagy induced by lack of blood supply plays a protective effect during periods of ischemia. For example, see [43], autophagy is significantly up-regulated during chronic ischemia in the pig heart. In this model, the level of autophagy was inversely correlated with that of apoptosis in the ischemic area. The ischemic area was recovered when the coronary flow was restored, suggesting that autophagy may protect myocardium from apoptosis during hibernation. Inhibition of endogenous AMPK suppressed autophagy during prolonged ischemia, which was accompanied by enlargement of the myocardial infarction. Although one may speculate that activation of autophagy may be reverted as soon as ischemia is relieved, the level of autophagy in fact further increases during reperfusion. However, a higher level of autophagy is not due to the lack of energy during blood flow restoration after reperfusion. Mechanisms mediating autophagy during reperfusion appear different from those involved in autophagy during ischemia. Energy crisis, a major stimulus for autophagy, in the heart is at least partially resolved at the time of reperfusion [32]. Instead, ROS appears to be a major promoter of autophagy during reperfusion. ROS induces mitochondrial damage, as evidenced by mitochondrial permeability transition pore (mPTP) opening and mitochondrial fragmentation, which in turn promotes autophagy and/or mitophagy, a specialized form of autophagy which removes mitochondria [44]. ROS oxidizes and inhibits the cysteine protease activity of Atg4, which results in LC3 lipidation and autophagy.
Whether autophagy induced during reperfusion is beneficial or detrimental remains controversial. Previous data [32] have shown that, although autophagic flux is inhibited during ischemia/reperfusion, enhancing autophagic flux during ischemia/reperfusion protects against ischemia/reperfusion injury in cardiomyocytes in vitro. The number of Bax (+) cardiac cells induced by reperfusion was significantly increased when Beclin-1 or Atg-5 were knocked out, but was reduced when there was an overexpression of Beclin-1 [45]. In an in vivo model of myocardial ischemia/reperfusion in pigs, autophagy was significantly activated when the coronary perfusion is restored, which was accompanied by reduction of apoptosis in myocardial cells and almost complete recovery of cardiac function [46]. Other experiments have also demonstrated that autophagy activation during myocardial ischemia/reperfusion can remove the damaged mitochondria caused by Bnip3. These results indicate that autophagy may promote cell survival during myocardial ischemia/reperfusion injury.
In contrast, study in reference [30] showed that inhibiting autophagy by treatment with 3-methyladenine or by Beclin1 knock down increases the survival of cardiomyocytes after ischemia/reperfusion in vitro. As results in [22], the myocardial infarct size increased by 40% in rats subjected to reperfusion, while the infarct size was down-regulated to 20% after the autophagy gene Beclin1 knockout. Inhibition of cathepsin, which can degrade autophagosomes, can significantly reduce myocardial cell damage and apoptosis during the reperfusion phase. Therefore, the roles of autophagy are not very clear during myocardial reperfusion. Further studies are needed to investigate the real roles of autophagy in myocardial ischemia/reperfusion injury. However, it is still speculated that the excessive degradation of important proteins and organelles by autophagy will cause cell death.
8. The role of autophagy in aging hearts subjected to ischemia/reperfusion
Aging is characterized by a progressive accumulation of damaged cells and organs. Autophagy degraded damaged organelles and macromolecule materials in stress, which prolonged life span [47]. Autophagy, including the autophagosome formation, the maturation, and the efficiency of autophagosome-lysosome fusion, as well as the proteolysis activity of lysosomes, declines with age [48]. All of these factors induce an abundance of the lipofuscin polymer accumulated in lysosomes. The lipofuscin polymer could not be degraded by lysosomes. The lysosome with an abundance of lipofuscin polymer lost its bio-function. Therefore, some damaged organelles and macromolecule materials could not be cleaned up, which accounted for the aging process. Several studies supported enhancing autophagy as the most efficient anti-aging intervention [49, 50].
In the heart, autophagy maintains a low basal level to perform biological functions, such as degrading dysfunctional organelles, maintaining cardiac morphology and function [51, 52]. However, autophagy in cardiomyocytes were up-regulated in response to environmental stress conditions, such as ATP depletion (e.g. during starvation), oxidative damage, and mitochondrial permeability transition pore opening (e.g. myocardial ischemia/reperfusion injury) [53, 54]. Activation of autophagy during ischemia is essential for cell survival and maintenance of cardiac function. However, autophagy was activated in the heart subjected to ischemia/reperfusion. Recent reviews show autophagy during reperfusion could be either protective or detrimental [41]. Serious induction of autophagy accompanied by robust up-regulation of Beclin-1 could cause autophagic cell death, thereby proving to be detrimental. If ischemia is mild, activation of autophagy during reperfusion may be modest and thus may not be harmful.
Until now, there is little information related to autophagy in myocardial ischemia/reperfusion injury in aging. Our primary data showed that ischemia/reperfusion injury was more serious in aging hearts compared with young hearts. Beclin-1 was increased in aging hearts subjected to ischemia/reperfusion, which indicated activated autophagy. However, further research is needed to know the exact role of autophagy in myocardial ischemia/reperfusion injury, especially in aging. Moreover, recent studies suggest that autophagy is one of the important mechanisms in myocardial ischemia/reperfusion preconditioning. Decreased autophagy may contribute to the weakened protective role of preconditioning in aging hearts subjected to ischemia/reperfusion. Therefore, excessive autophagy results in autophagic cell death and loss of cardiomyocytes, responsible for the worsening of aging myocardial ischemia/reperfusion injury. The molecular transduction pathways need to be investigated further. It could help to develop therapies that up-regulate the repair qualities of the autophagic process and down-regulate the cell death aspects, which would be of great value in the treatment of aging myocardial ischemia/reperfusion injury.
9. The investigative methods of autophagy
9.1. Electron microscopy
Electron microscopy is the most reliable method for testing autophagy at present and can be used to quantify the autophagic activity of cells. In electron microscopy, the autophagosome is composed of double membrane structures that wrap abnormal cytoplasmic material. Then, the autolysosome is composed of the monolayer membrane structure which wraps cytoplasm ingredients at different degradation stages. Because of the difficulty of distinguishing between autophagosomes and autolysosomes, both of them are often called ‘autophagic vacuoles’. The proportion of autophagic vacuoles is calcultaed accounting for the total area or volume of cytoplasm through electron microscopy, which can be used to quantify cell autophagic activity.
9.2. Specific markers
Atg8 includes three kinds of homology in humans: GABARAP, GATE-16 and LC3. The C-terminal proteolysis of LC3 is processed by Atg4, based on the residues Phe80 and Leu82 of LC3 that may be recognized by Atg4, and immediately follows synthesis to yield a soluble form, LC3-I. LC3-I is converted to a membrane bound form, LC3-II, through a ubiquitin-like reaction involving Atg7, a ubiquitin-acivating enzyme (E1)-like enzyme, and Atg3, a ubiquitin-conjugating enzyme (E1)-like enzyme (E2). LC3-II combines to phosphatidyl ethanolamine (PE) on the membrane surface of autophagic vacuoles. In addition, LC3-II is easily located within the cell after the formation of the fusion protein with the green fluorescent protein (GFP). Therefore, GFP-LC3 II is usually used as the marker protein of the autophagosome membrane in mammalian cells.
Beclin 1 is involved in the formation of autophagosomes, which are the mammalian yeast homologues of Apg6/Vps30. Some researches show that autophagy is significantly attenuated in Beclin 1+/- mice, but apoptosis is normal. These results indicate that Beclin 1 is a significant positive control gene in autophagy. Therefore, by testing the expression level of Beclin1, combined with other biochemistry indexes, the autophagic activity of cells can be monitored and judged dynamically.
9.3. Monodansylcadaverine (MDC) dyeing
MDC is a kind of fluorescent dye, which is used as a tracer of the autophagic vacuoles. MDC can specifically bind to the ubiquitin-proteasome sample protein binding systems (Atg8). In addition, MDC can be absorbed by cells and selectively gathered in autophagic vesicles, showing punctuate structure under fluorescence microscope. Therefore, the quantitative detection of autophagy uses this method. However, most of the MDC is not marked in GFP-LC3. However, MDC is not a reliable marker of autophagosomes. Therefore, other experimental evidences that represent the autophagic activity of cells are needed.
9.4. Specific agonists and inhibitors
Rapamycin is a novel macrolide immunosuppressant, and induces cell autophagy by inhibiting the mTOR pathway. In scientific studies, Rapamycin is the specific agonist of autophagy. The main inhibitors of the phosphor-lipin acid radical inositol 3 kinase include 3-MA, which can specifically block the fusion of autophagic vacuoles and lysosomes. Rapamycin is widely used as an inhibitor of autophagy, and Wortmannin, LY29400297 and Bafilomycin A1 are included.
10. Perspectives
Autophagy is intimately involved not only in the physiology of the heart, but also in development of the ischemic heart. The regulation of autophagy may be a new approach for the treatment of ischemic heart disease. However, to translate the knowledge of autophagy into treatment of ischemic heart disease, it is necessary to know more precisely about the formation, function, mechanism, and regulation of autophagy. When autophagy is protective and when it is detrimental for the ischemic heart needs further clarification. Another problem is how to regulate autophagy without affecting other life activities, since even the evolutionarily conserved autophagy genes also have autophagy-dependent functions. There is continuing research on this topic. In addition, the signaling mechanisms positively or negatively regulating autophagy in the heart have not been completely elucidated. Judging from the diversity of autophagy regulators, it is believed that more unknown signal transduction pathways will also be proven to be involved in the activation of autophagy. In short, only clarifying the activation mechanism, function, time course, and its relationship with cell death, etc. autophagy can truly benefit patients with ischemic heart disease.
Acknowledgements
This work was supported by the grants from the Natural Sciences Foundation of China (NSFC) 81270283, the NSFC 81070263, the NSFC 30973163, KZ201110025023 from Science and Technology Plan Project of Beijing Municipal Education Commission, and the Funding Project for Academic Human Resources Development in Institutions of Higher Learning under the Jurisdiction of Beijing Municipality (PHR201106112).
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Introduction",level:"1"},{id:"sec_2",title:"2. Definition, formation and classification of autophagy ",level:"1"},{id:"sec_3",title:"3. Signaling regulation of autophagy",level:"1"},{id:"sec_4",title:"4. The role of autophagy in the maintenance of normal myocardium",level:"1"},{id:"sec_5",title:"5. The protective effect of autophagy in myocardial ischemia/hypoxia",level:"1"},{id:"sec_6",title:"6. The expression and regulation of autophagy during myocardial ischemia/reperfusion",level:"1"},{id:"sec_6_2",title:"6.1. The role of Beclin 1 in the occurrence of autophagy during myocardial ischemia/reperfusion",level:"2"},{id:"sec_7_2",title:"6.2. The role of Bcl-2 family members in the occurrence of autophagy during myocardial ischemia/reperfusion",level:"2"},{id:"sec_7_3",title:"6.2.1. Bnip3",level:"3"},{id:"sec_8_3",title:"6.2.2. Bcl-2/Bcl-XL and Bax",level:"3"},{id:"sec_10_2",title:"6.3. The role of angiotensin II (Ang II) receptor signaling in the induction of autophagy during myocardial ischemia/reperfusion",level:"2"},{id:"sec_12",title:"7. The role of autophagy in myocardial ischemia/reperfusion",level:"1"},{id:"sec_13",title:"8. The role of autophagy in aging hearts subjected to ischemia/reperfusion",level:"1"},{id:"sec_14",title:"9. The investigative methods of autophagy",level:"1"},{id:"sec_14_2",title:"9.1. Electron microscopy",level:"2"},{id:"sec_15_2",title:"9.2. Specific markers",level:"2"},{id:"sec_16_2",title:"9.3. Monodansylcadaverine (MDC) dyeing",level:"2"},{id:"sec_17_2",title:"9.4. Specific agonists and inhibitors",level:"2"},{id:"sec_19",title:"10. Perspectives",level:"1"},{id:"sec_20",title:"Acknowledgements",level:"1"}],chapterReferences:[{id:"B1",body:'Ashford TP, Porter KR. Cytoplasmic components in hepatic cell lysosomes. J Cell Biol 1962;12(1) 198–202.'},{id:"B2",body:'Klionsky DJ. Autophagy revisited: a conversation with Christian de Duve. 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Proc Natl Acad Sci USA 2005;102(39) 13807-13812.'},{id:"B44",body:'Scherz-Shouval R, Elazar Z. ROS, mitochondria and the regulation of autophagy. Trends Cell Biol. 2007;17(9) 422-427.'},{id:"B45",body:'Hamacher-Brady A, Brady NR, Gottlieb RA: Enhancing macroautophagy protects against ischemia/reperfusion injury in cardiac myocytes. J Biol Chem 2006;281(40) 29776-29787.'},{id:"B46",body:'Javier A. Sala-Mercado, Joseph Wider, Vishnu Vardhan Reddy Undyala, Salik Jahania, Wonsuk Yoo, Robert M. Mentzer, Jr, Roberta A. Gottlieb, and Karin Przyklenk. Profound Cardioprotection with Chloramphenicol Succinate in the Swine Model of Myocardial Ischemia-Reperfusion Injury. Circulation 2010;122(Suppl 11) S179-S184.'},{id:"B47",body:'Xie Z, Klionsky DJ. Autophagosome formation: core machinery and adaptations. Nat Cell Biol 2007;9(10) 1102-1109.'},{id:"B48",body:'M, Yamaguchi O, Nakai A, Hikoso S, Takeda T, Mizote I, Oka T, Tamai T, Oyabu J, Murakawa T, Nishida K, Shimizu T, Hori M, Komuro I, Shirasawa T, Mizushima N, Otsu K. Inhibition of autophagy in the heart induces age-related cardiomyopathy. Autophagy 2010;6(5) 600-606.'},{id:"B49",body:'Dong Y, Undyala VV, Gottlieb RA, Mentzer RM Jr, Przyklenk K. Autophagy: definition, molecular machinery, and potential role in myocardial ischemia-reperfusion injury. J Cardiovasc Pharmacol Ther 2010;15(3) 220-230. '},{id:"B50",body:'De Meyer GR, De Keulenaer GW, Martinet W. Role of autophagy in heart failure associated with aging. Heart Fail Rev 2010;15(5) 423-430. '},{id:"B51",body:'Cuervo AM, Bergamini E, Brunk UT, Dröge W, Ffrench M, Terman A. Autophagy and aging: the importance of maintaining "clean" cells. Autophagy 2005;1(3) 131-140. '},{id:"B52",body:'Gottlieb RA, Finley KD, Mentzer RM Jr. Cardioprotection requires taking out the trash. Basic Res Cardiol 2009;104(2) 169-180.'},{id:"B53",body:'Gustafsson AB, Gottlieb RA. Autophagy in ischemic heart disease. Circ Res 2009;104(2) 150-158.'},{id:"B54",body:'Nishida K, Kyoi S, Yamaguchi O, Sadoshima J, Otsu K. The role of autophagy in the heart. Cell Death Differ 2009;16(1) 31-38.'}],footnotes:[],contributors:[{corresp:null,contributorFullName:"Suli Zhang",address:null,affiliation:'
Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, Beijing, P.R. China
Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, Beijing, P.R. China
'}],corrections:null},book:{id:"3435",title:"Ischemic Heart Disease",subtitle:null,fullTitle:"Ischemic Heart Disease",slug:"ischemic-heart-disease",publishedDate:"February 15th 2013",bookSignature:"David C. Gaze",coverURL:"https://cdn.intechopen.com/books/images_new/3435.jpg",licenceType:"CC BY 3.0",editedByType:"Edited by",editors:[{id:"71983",title:"Dr.",name:"David C.",middleName:null,surname:"Gaze",slug:"david-c.-gaze",fullName:"David C. Gaze"}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"},chapters:[{id:"42788",title:"Introduction to Ischemic Heart Disease",slug:"introduction-to-ischemic-heart-disease",totalDownloads:2889,totalCrossrefCites:0,signatures:"David C. Gaze",authors:[{id:"71983",title:"Dr.",name:"David C.",middleName:null,surname:"Gaze",fullName:"David C. 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1. Introduction
As people age, their bodies begin to deteriorate. Understanding how changes in the DNA of aging people affect cellular function will be an important clue for future prevention and treatment of age-associated noncommunicable diseases (NCDs). Genomic instability, a hallmark of cancer and aging, is defined as a high frequency of mutations within the genome [1, 2]. In cancer, the permanent alteration of the nucleotide sequence of DNA, or mutations, occurring in proto-oncogenes and tumor suppressor genes lead to cancer development and progression. In the aging process, however, the accumulation of DNA damage, which is an abnormal chemical structure in DNA and includes base modification, base loss, and DNA breaks (which are precursors of mutations), stimulates the DNA damage repair signal (DDR) to induce cells to repair DNA damage [3, 4]. Nevertheless, DDR arrests the cell cycle, rewires cellular metabolism, promotes senescence, and initiates programmed cell death. As a result, too much DDR drives the cellular aging process [3, 4]. Accumulation of DNA damage is found in the elderly and people with age-associated NCDs (Figure 1) [5]. Therefore, DNA damage accumulation is a crucial molecular pathogenic mechanism of the aging process. However, the mechanism by which DNA damage spontaneously accumulates in the aging genome remains to be explored.
Figure 1.
Genome-wide hypomethylation drives genomic instability in the elderly by reducing youth-associated genome-stabilizing DNA gaps: A hypothesis. DNA methylation in the elderly is generally reduced, genome-wide hypomethylation. A reduction in DNA methylation leads to genomic instability, accumulation of endogenous DNA damage, and sensitivity to DNA-damaging agents. Here, we propose a hypothesis that global hypomethylation causes a reduction in Phy-RIND-EDSBs and that the reduction in Phy-RIND-EDSBs causes DNA damage. The accumulation of endogenous DNA damage will promote DDR, and too much DDR will arrest cells, causing metabolic rewiring and senescence.
Both epigenetic marks and DNA damage or lesions are temporary modifications of DNA. However, both are produced by different mechanisms and play roles in genomic instability in opposite directions. Epigenetic marks are produced by biological processes and possess physiological functions [6, 7]. For example, DNA methylation or methyl CpG is produced by DNA methyltransferase. The molecular function of methyl CpG is to interact with a protein such as methyl-CpG-binding protein. This interaction forms a cascade of molecular biological processes for gene regulation control and genomic stability. DNA lesions, on the other hand, are produced by endogenous or exogenous hazards [8]. For example, pyrimidine dimers, one type of DNA lesion, are formed via photochemical reactions such as exposure to UV light. DNA damage is converted into a mutation during subsequent replication, so accumulation of DNA damage leads to genomic instability. This chapter describes that genomic instability in the elderly should occur by the alteration of epigenetic marks leading to spontaneous accumulation of DNA damage.
Global DNA hypomethylation is an epigenetic change in the elderly and people with NCDs that promotes genomic instability [9, 10, 11, 12, 13]. However, the underlying mechanism of how the hypomethylated genome accumulates DNA damage is unknown [14]. In 2008, my group discovered an unprecedented type of endogenous DNA double-strand break (EDSB). These breaks are found in all cells, including nondividing cells, so we named them replication-independent EDSBs (RIND-EDSBs) [15]. RIND-EDSBs are located in hypermethylated DNA. Therefore, cells with global hypomethylation, such as cancer cells, have lower levels of RIND-EDSBs than noncancer cells [15]. After the discovery, we explored several characteristics of RIND-EDSBs and found that the majority of RIND-EDSBs possess physiological functions, namely, physiologic RIND-EDSBs (Phy-RIND-EDSBs), as epigenetic marks in maintaining genomic stability [16, 17, 18, 19]. Interestingly, Phy-RIND-EDSBs in yeast decrease when yeast cells age [19]. So here I rename Phy-RIND-EDSBs in accordance with their role as youth-associated genomic-stabilizing DNA gaps (Youth-DNA-GAPs). In this chapter, we propose a hypothesis that the hypomethylated genome of the elderly reduces Phy-RIND-EDSBs and that this reduction causes DNA damage. The accumulation of DNA damage initiates DDR and consequently drives the cellular aging process (Figure 1). In other words, the reduction in Phy-RIND-EDSBs by genome-wide hypomethylation is the underlying molecular pathogenesis mechanism of aging phenotypes.
2. Genome-wide hypomethylation
Genome-wide hypomethylation reduces the DNA methylation level of the whole genome. DNA methylation possesses two basic roles, gene regulation and the prevention of genomic instability, which we emphasize here [20]. The majority of DNA methylation in the human genome is on interspersed repetitive sequences (IRSs). Genome-wide hypomethylation or global hypomethylation mostly reflects a decrease in the DNA methylation of IRSs [11, 21]. Here, I will describe how IRS methylation occurs, how hypomethylation occurs, and how hypomethylation drives genomic instability in the elderly.
2.1 Interspersed repetitive sequence methylation
To evaluate the global methylation level, most recent studies have used PCR techniques to measure the DNA methylation level of each IRS, including Alu elements (Alu), long interspersed element-1s (LINE-1s), and several types of human endogenous retroviruses (HERVs). A reduction in Alu element methylation represents a genome-wide hypomethylation, driving genomic instability more than that of LINE-1 s and HERVs [11]. Throughout the human genome, there are over 1 million copies of Alu elements [22]. Although there is also a vast number of LINE-1 s, only approximately 3000 copies of LINE-1s contain a 5’ UTR where LINE-1 methylation was usually measured [23, 24]. Because there are several classes of HERVs, each PCR measured DNA methylation of one class and as a result, covered a smaller percentage of the genome [25]. Furthermore, methylation of LINE-1 and HERV was reported to possess gene regulation functions [24, 26]. The tissue-specific methylation level of LINE-1 is locus dependent [27, 28]. In contrast, the global hypomethylation occurs as a generalized process [11, 28]. Therefore, methylation of LINE-1 and HERV represents global methylation in a lesser proportion than that of Alu elements.
2.2 Alu hypomethylation in aging and NCDs
Although global hypomethylation has been reported in the elderly, not all IRSs are hypomethylated. We investigated Alu, LINE-1, and HERV-K and found Alu and HERV-K hypomethylation in aging but not LINE-1 [11]. Therefore, methylation of LINE-1 and Alu may possess different roles. Global hypomethylation is also associated with the aging phenotype. First, lower global DNA methylation is associated with higher cardiovascular risk in postmenopausal women [29]. Second, Alu hypomethylation was observed in individuals with lower bone mass, osteopenia, osteoporosis, and a high body mass index [12]. Finally, Alu hypomethylation was reported in diabetes mellitus patients and was directly correlated with high fasting blood sugar, HbA1C, and blood pressure [13]. Interestingly, the Alu methylation level was also high in catch-up growth in a 20-year-old offspring [30]. These studies indicated the positive role of Alu methylation in the human growth process and the role of Alu hypomethylation as an epigenetic cause of the human aging process.
2.3 Mechanism causing global hypomethylation
The direct correlation between IRS methylation levels suggests that the mechanisms causing global hypomethylation in both aging cells and cancer are a generalizing process [11, 28]. The actual mechanism causing global hypomethylation in aging remains to be explored. Nevertheless, exposure to oxidative stress, benzene, air pollution, UV light, radiation, smoke, and folate deficiency facilitates genome-wide hypomethylation processes [31, 32, 33, 34, 35, 36, 37]. Therefore, the accumulation of DNA damage, oxidative stress, or a lack of DNA methylation precursors can lead to genome-wide hypomethylation.
Evidence suggests that DNA damage drives the demethylation process. DNA repair, which is how cells remove DNA lesions, is also a demethylation mechanism that directly removes 5-methylcytosine. Methylcytosine is a DNA base that is prone to be deaminated and must be fixed by base excision repair (BER) machinery to prevent cytosine-to-thymine substitution. However, BER replaces the DNA lesion with an unmethylated form of cytosine. As a result, the methylcytosine is demethylated. The other mechanism is to remove the entire DNA patch and refill with unmethylated nucleotides by nucleotide excision repair (NER) or mismatch repair (MMR) [38].
For oxidative stress, oxidation of 5-methylcytosine forms 5-hydroxymethylcytosine. There are several mechanisms for removing 5-hydroxymethylcytosines and replacing them with unmethylated forms, AID/APOBEC enzymes and TET enzymes followed by BER [39, 40, 41, 42, 43, 44]. Alternatively, oxidative stress may interfere with the DNA methylation protein machinery. For example, oxidative stress depletes the synthesis of glutathione and decreases the availability of S-adenosylmethionine for DNA methylation [45]. This proposed mechanism is similar to DNA demethylation in depletion of the methyl pool in folate-deficient models [46, 47].
2.4 Hypomethylation accumulates multiple kinds of DNA lesions
The hypomethylated genome is prone to accumulating multiple kinds of DNA damage, which is an abnormal chemical structure in DNA and includes oxidative damage, depurination, depyrimidination, and pathologic EDSBs [10, 14]. Alu methylation levels in white blood cells were found to inversely correlate with 8-hydroxy-2′-deoxyguanosine (8-OHdG) oxidative damage and apurinic/apyrimidinic sites (AP sites) [37]. Transfection of cells with Alu small interfering RNA (Alu siRNA) increased Alu methylation and reduced endogenous 8-OHdG and AP sites [37]. Interestingly, Alu siRNA also increased cell division and resistance to DNA damage-causing agents [37]. This evidence indirectly suggests that Alu methylation stabilizes the human genome. DNA methylation also prevents pathologic EDSBs. The chromosomal rearrangements and deletions of DNA commonly found in cancer cells treated with DNA demethylating agents and DNA methyltransferase (DNMT) knockout mice and naturally occurring mutations in the cytosine DNA methyltransferase DNMT3B suggest that pathologic EDSBs are the intermediate products of hypomethylation that drive genomic instability [10, 48, 49, 50].
2.5 DNA lesions as a molecular pathogenesis mechanism of the aging process and NCDs
A number of studies support the idea that accumulation of DNA damage drives the aging process. First, congenital defects in DNA repair accelerate aging. For example, progeroid syndrome patients with ERCC4 mutations have premature aging of many organs. ERCC4 is a protein designated as the DNA repair endonuclease XPF that is critical for many DNA repair pathways, including NER [51]. Second, genotoxic agents accelerate the aging process in cancer survivor patients. For example, 50-year-old survivors of childhood cancer have an increased incidence of age-related diseases compared to their siblings [52]. Third, there is evidence of DNA damage accumulation when cells age. Pathologic EDSBs are accumulated in chronological aging yeast [17]. Many kinds of DNA damage from base modifications to γH2AX foci, representing pathologic EDSBs, have been reported in several organs of animals and humans [53, 54, 55, 56]. Finally, a reduction in DNA repair efficiency was reported in aging cells of many organisms [57, 58, 59]. In NCDs, the accumulation of oxidative DNA damage has been reported in patients with cardiovascular disease, diabetes and metabolic syndrome, chronic obstructive pulmonary disease, osteoporosis, and neurological degeneration, including Alzheimer’s disease and Parkinson’s disease [5]. DNA damage triggers DDR. To facilitate DNA repair and prevent mutation accumulation, DDR arrests cell cycle progression until repair is complete. While DDR can prevent cancer development, DDR leads to many unwanted effects, including inflammation, metabolic rewiring, senescence, apoptosis, and aging [60, 61, 62]. The DDR signaling pathway consists of signal sensors, transducers, and effectors. The sensors of this pathway are proteins that recognize DNA damage. The main transducers are ATM and ATR and their downstream kinases. The effectors of this pathway are substrates of ATM and ATR and their downstream kinases. These effectors of DDR involve many proteins, including P53, BRCA1, and CDC25s [60, 61, 62, 63].
2.6 DNA methylation possesses a long-range effect in stabilizing the human genome in cis
A direct association between loss of DNA methylation and rearrangements in the pericentromeric heterochromatin was demonstrated in ICF syndrome (immunodeficiency, chromosomal instability, and facial anomalies) and loss-of-function mutations in DNMT3B [50, 64]. Therefore, hypomethylation could lead to spontaneous mutations in cis, which are epigenetic and genetic events occurring in the same chromosome. Notably, Alu siRNA increased Alu methylation levels in HEK293 cells from 60 to 70% [14]. Because there are approximately 1 million copies of Alu, by rough estimation, Alu siRNA methylates 10% of Alu elements or approximately 100,000 Alu elements in 3000 Mb of the human genome. In other words, Alu siRNA transfection methylated one locus of every 30 kb of human genome on average. Furthermore, Alu siRNA reduced 75% of endogenous 8-OHdG [14]. Therefore, even if Alu siRNA increases methylation in a limited location, the transfection stabilized the genome far beyond the methylated Alu elements (Figure 2).
Figure 2.
DNA methylation possesses a long-range effect in stabilizing the human genome in cis. This diagram represents a fraction of the human genome before and after Alu siRNA transfection. While Alu siRNA methylated only 10% of Alu loci, Alu siRNA reduced 75% of the 8-OHdG in the entire genome [14]. Therefore, DNA methylation possesses a long-range effect in stabilizing the human genome. Blue circles are DNA methylation and white circles are unmethylated DNA.
2.7 Hypotheses: DNA methylation prevents genomic instability mechanisms
There are at least three possible mechanisms by which Alu methylation reduces endogenous DNA damage and increases resistance to DNA damage-causing agents. The extension of genomic stability from methylated Alu loci supports my first hypothesis that DNA methylation stabilizes the genome by homing Youth-DNA-GAPs, Phy-RIND-EDSBs, and that the gaps extended the stabilizing effect to the entire genome [14]. Another reason that supports the Phy-RIND-EDSBs mediating the DNA methylation role in stabilizing the genome is that Phy-RIND-EDSBs are localized in hypermethylated DNA [15]. Moreover, Phy-RIND-EDSBs possess a redundant topoisomerase which relieve tension of double-helix spin and torsion from any DNA activity [17]. The second hypothesis would be the spreading of DNA methylation and consequently heterochromatin [65]. However, this mechanism is unlikely because the spreading would need to extend to cover the whole genome and would interfere with cellular function. A reduction in cell viability by Alu siRNA was not observed. The last and unlikely hypothesis was that DNA methylation somehow enhanced DNA repair activity [66], although this mechanism is also unlikely because most DNA repair machinery starts with specific sensors to recognize DNA lesions.
3. Phy-RIND-EDSBs represent epigenetic marks as youth-DNA-GAPs
Phy-RIND-EDSBs are found in all eukaryotic cells, produced by certain proteins, and reduced in chronological aging yeast [15, 17, 19]. A reduction in Phy-RIND-EDSBs decreased cell viability and augmented pathologic EDSB production [19]. Phy-RIND-EDSBs are devoid of DDR and are repaired by the error-free repair pathway [16]. Therefore, Phy-RIND-EDSBs are Youth-DNA-GAPs epigenetic marks that prevent genomic instability in eukaryotic genomes.
3.1 IRS-EDSB ligation-mediated PCR (IRS-EDSB-LMPCR) to measure EDSBs
Ligation-mediated PCR (LMPCR) is the method that we used for EDSB detection [15]. Previously, this PCR technique was used to characterize the signal end and coding end of EDSBs occurring during the V(D)J recombination process [67]. For V(D)J recombination, the signal end and coding end of EDSBs occur at the T-cell receptor or antibody genes in lymphoblasts. To detect the signal end and coding end, DNA from lymphoblasts was ligated to a linker, and PCR was performed using linker primer and oligonucleotide sequences of T-cell receptor or antibody genes. Generalized EDSBs can occur anywhere in the genome. Therefore, we replaced IRS as a primer instead of T-cell receptor or antibody genes [67, 68]. As a result, IRS-EDSB-LMPCR yields two types of amplicons, IRS-EDSB and IRS-IRS sequences, and we detected linker sequences that represent EDSB amplicons. In brief, IRS-EDSB-LMPCR was performed as follows. First, the oligonucleotide linker, EDSB linker, was ligated to high-molecular-weight DNA (HMWDNA) or nucleus. Second, real-time quantitative PCR was performed using two PCR primers. The first was homologous to IRS, and the other had the same sequence as the 5′ end of the ligation linker. The number of EDSBs could be measured by Taqman probe homology to the 3′ end of the ligation linker sequence. The HMWDNA or nucleus served as a source of EDSBs, and the EDSB linker detected and ligated EDSBs. The first PCR cycle polymerized DNA from genome-wide distributed IRSs. The polymerization through EDSBs generated an EDSB-LMPCR linker template. The IRS-EDSB-linker sequences were generated, detected, and quantitated by the Taqman probe during PCR cycle (Figure 3) [15].
Figure 3.
IRS-EDSB-LMPCR diagram demonstrating IRS-EDSB-LMPCR. LMPCR linker ligates to EDSB. The 5′ end of the LMPCR linker is the same sequence as the PCR primer. The 3′ end of the LMPCR linker is homologous to the Taqman probe. The Taqman probe is used for quantitation of EDSBs by real-time PCR. The IRS primer is a PCR primer with IRS sequences to polymerize numerous locations of the genome [15].
Common criticism of IRS-EDSB-LMPCR is the possibility of DNA shearing from HMWDNA preparation. However, the characteristics of the DSBs generated by DNA preparation are different from RIND-EDSBs. In humans, the sequence around RIND-EDSBs is always hypermethylated, whereas methylation levels of DSBs from mechanical shearing possess less methylation than RIND-EDSBs [15]. To prove that the RIND-EDSBs are real, we compared EDSBs from linker ligated to HMWDNA and nucleus and found that RIND-EDSBs analyzed directly from in situ ligation displayed the same pattern as IRS-EDSB-LMPCR from HMWDNA [17]. Therefore, DSBs detected by IRS-EDSB-LMPCR were endogenous in origin.
3.2 Phy-RIND-EDSBs are evolutionarily conserved epigenetic marks
Nature has conserved all epigenetic marks by conserving the genes that produce epigenetic marks [7]. Epigenetic marks have a specific biological role, whether it is gene expression, genomic stability, or interacting with DNA. Therefore, the genome distribution of epigenetic markers will not be random. Finally, epigenetic marks are usually crucial for cell survival and therefore should be ubiquitously present in all cells. To search for genes that produce or maintain Phy-RIND-EDSBs, we evaluated RIND-EDSB levels in yeast strains that lack functional mutation genes encoding various DNA repair regulators, chromatin formation, endonucleases, topoisomerase, and chromatin-condensing proteins [17]. We found low levels of RIND-EDSBs in cells lacking high-mobility group box (HMGB) proteins and Sir2. Thus, HMGB proteins and Sir2 play roles in producing and maintaining Phy-RIND-EDSBs [17]. Phy-RIND-EDSBs are distributed in the genome nonrandomly [18]. In humans, Phy-RIND-EDSBs are localized within hypermethylated DNA [15]. In yeast, DNA sequences 5′ end to RIND-EDSBs were not random; certain four-nucleotide sequences were more likely to be present immediately prior to the breaks. Moreover, RIND-EDSBs were prevented from occurring or were never observed following certain four-base combinations [18]. RIND-EDSBs were found in yeast and in the human genome, and therefore, Phy-RIND-EDSBs are conserved in eukaryotic organisms [15, 17]. In humans, RIND-EDSBs were detectable in all cell types and found within the hypermethylated genome in all phases of the cell cycle [15]. In yeast, we found a very strong direct correlation between cell viability and Phy-RIND-EDSB levels (r = 0.94, p < 0.0001) [19]. In other words, the more Phy-RIND-EDSBs a cell possesses, the better the cell survives [19]. When Phy-RIND-EDSB levels were reduced by homothallic switching (HO) endonuclease induction or NHP6A gene deletion, cell viability decreased [19]. In conclusion, Phy-RIND-EDSBs are epigenetic markers that are important in all eukaryotic cells [19].
Most of the RIND-EDSBs under normal physiologic conditions are not DNA damage, signals of the DDR, or precursors of mutations [16]. While sequences around human RIND-EDSBs are hypermethylated, γH2AX-binding DNA is hypomethylated. Therefore, most RIND-EDSBs are devoid of γH2AX [16]. γH2AX is a H2AX molecule that is phosphorylated at serine 139 by the signaling cascade of DDR of pathologic DSBs [69]. Most RIND-EDSBs are repaired by a more precise ATM-dependent pathway, and therefore, most RIND-EDSBs under normal physiologic conditions are Phy-RIND-EDSBs [16].
3.3 Phy-RIND-EDSB or youth-DNA-GAP complex
Human Phy-RIND-EDSBs are localized in hypermethylated DNA regions and deacetylated histones [15, 16]. Phy-RIND-EDSBs are reduced in cells lacking HMGB proteins and Sir2 and NAD-dependent deacetylase [17, 70]. The human Sir2 homolog, sirtuin 1 (SIRT1), binds to the HMGB1 protein and deacetylates DNMT1 [71, 72]. Furthermore, HMGB1 possesses deoxyribophosphate lyase activity [73]. Therefore, we propose a hypothesis here that HMGB1 cuts DNA to produce Phy-RIND-EDSBs. SIRT1-bound HMGB1 deacetylates histones, keeping Phy-RIND-EDSB ends within the heterochromatin to shield them from the DDR signal. Finally, the interaction between SIRT1 and DNMT1 or deacetylated histone and DNA methylation may be the reason why sequences around human Phy-RIND-EDSBs are hypermethylated (Figure 4).
Figure 4.
Phy-RIND-EDSB or youth-DNA-GAP complex: I hypothesize that the HMGB group initiates Phy-RIND-EDSB and that HMGB interacts with SIR2 or SIRT1. SIRT1 deacetylates histones and DNMT1, and DNMT1 methylates DNA. The role of Phy-RIND-EDSB or youth-DNA-GAP is to prevent DNA damage anywhere along the same chromosome.
Interestingly, both HMGB1 and Sir2 have other functions that can be related to Phy-RIND-EDSBs. HMGB1 is a protein in another physiologic EDSB complex, the signal end and coding end of V(D)J recombination [74]. Moreover, yeast lacking the NHP6A protein, a type of yeast HMGB, shows increased endogenous DNA damage and sensitivity to UV light [75]. Finally, HMGB1 has the ability to bend DNA [76]. To prevent DNA torsion, it is reasonable to create Phy-RIND-EDSBs while bending DNA. Sir2 can deacetylate the histone, while Phy-RIND-EDSBs are localized in the deacetylated histone. Interestingly, Sir2 and SIRT1 are known to prevent the aging process [77, 78].
3.4 Spontaneous pathologic RIND-EDSBs and modified ends with insertion at the breaks
Independent of DNA replication, EDSB-LMPCR could detect pathologic RIND-EDSBs (Path-RIND-EDSBs) as excess EDSBs when DSB repair was inhibited by chemical inhibition or DSB repair gene mutation [19]. When we treated G0 yeast cells with caffeine, a DSB repair inhibitor, we observed a spontaneous increase in RIND-EDSBs [19]. These excess RIND-EDSBs did not possess the same 5′ end-sequence-four-base combinations as Phy-RIND-EDSBs odds ratio (OR) > 1 breaks. Notably, we called four-base combinations that are unlikely to be found in Phy-RIND-EDSBs as OR ≤ 1 breaks [18]. Moreover, we also observed that the 5′ end sequence downstream of the break did not match with the genomic sequence from the first base as reads with modified ends with insertion at the breaks (MIBs) [57]. We found that caffeine treatment increased the proportion of MIBs [57]. Therefore, MIBs might be a mechanism that compensates for repair defects, such as alternate repair of the DSB pathway, or prevents EDSB ends from stimulating DDR. Seven repair defect yeast strains, mec1Δ, mre11Δ, nej1Δ, rad51Δ, tel1Δ, yku70Δ, and yku80Δ, were studied. Except for nej1Δ, the percentages of OR ≤ 1 breaks and MIBs were significantly increased in all samples when compared to the wild type. We also examined whether there was an association between MIBs and types of breaks (OR > 1 breaks and OR ≤ 1 breaks) and found that in the wild type, MIBs occurred at OR ≤ 1 breaks. In contrast, in mec1Δ, mre11Δ, rad51Δ, tel1Δ, yku70Δ, MIBs occurred at both OR > 1 breaks and OR ≤ 1 breaks [57]. Therefore, both Phy-RIND-EDSBs and Path-RIND-EDSBs are produced in the genome independent of DNA replication. However, most Path-RIND-EDSBs are immediately repaired, while Phy-RIND-EDSBs are retained [57].
3.5 Variation in RIND-EDSB level and reduction in Phy-RIND-EDSBs in aging and hypomethylated cells
Path-RIND-EDSBs are spontaneously produced and immediately repaired, while Phy-RIND-EDSBs are produced and retained by the Phy-RIND-EDSB complex formation process. We observed an increase in RIND-EDSB levels in yeast lacking a DSB repair gene, topoisomerase and endonuclease. Analysis of EDSB sequences suggested that DSB repair inhibition causes retention of both Phy-RIND-EDSBs and Path-RIND-EDSBs. For topoisomerase and endonuclease mutants, we postulated that Phy-RIND-EDSBs may have redundant roles with topoisomerase and endonuclease in stabilizing the genome. Nevertheless, sequence analysis is needed to prove this hypothesis. As mentioned earlier, one yeast strain lacking the HMGB gene or SIR2 possessed a low level of RIND-EDSBs. Therefore, we hypothesized that HMGB and Sir2 play roles in PHY-RIND-EDSB complex formation and retention. We observed that three chemicals can alter RIND-EDSB levels. Whereas caffeine and vanillin, DSB repair inhibitors, increased RIND-EDSB levels, trichostatin A, a histone deacetylase inhibitor, decreased the EDSBs. The reduction in RIND-EDSBs by trichostatin A suggested that Phy-RIND-EDSBs are retained within facultative heterochromatin. This result is similar to the low level of RIND-EDSBs in yeast lacking SIR2.
DDR signals to repair Path-RIND-EDSBs can repair and consequently reduce Phy-RIND-EDSBs [19]. The retention of Phy-RIND-EDSBs and the immediate repair of Path-RIND-EDSBs led to the finding that the majority of RIND-EDSBs under normal physiologic conditions are Phy-RIND-EDSBs and that a reduction in RIND-EDSBs in any condition is a reduction in Phy-RIND-EDSBs. In addition to gene mutation and histone acetylation, we could reduce RIND-EDSB levels in yeast by inducing a Path-RIND-EDSB by HO endonuclease induction [19]. HO endonuclease is a site-specific endonuclease that cleaves a site in the MAT locus on chromosome III [79]. After induction in nondividing yeast, we observed a sustained reduction in RIND-EDSBs for up to 4 days. However, when we induced HO in yeast lacking MEC1, a DSB repair protein, the reduction was not observed [19]. These experiments suggested that Path-RIND-EDSB production can ignite the global DSB repair process, and consequently, the retained Phy-RIND-EDSBs are repaired [19]. This mechanism is one possible explanation for the reduction in RIND-EDSBs in chronologically aging yeast.
Phy-RIND-EDSB levels in the elderly should be low. We found low levels of RIND-EDSBs in chronologically aging yeast and in the human cancer cells, HeLa and SW480, which are cervical cancer and colon cancer cell lines, respectively [15, 19]. Phy-RIND-EDSBs are localized in hypermethylated genomic regions [15]. Therefore, cancer genome hypomethylation may explain why RIND-EDSB levels in cancer cells were low [15, 27]. We have not reported RIND-EDSB levels in the elderly. However, our unpublished data demonstrated results similar to those in chronologically aging yeast and in cancer cells.
3.6 Reduction in Phy-RIND-EDSBs augments pathologic EDSB production
To define the molecular mechanism by which the reduction in Phy-RIND-EDSBs in chronological aging in yeast reduced cell viability and to evaluate the consequences of Phy-RIND-EDSB reduction, we analyzed yeast cells with low levels of Phy-RIND-EDSBs, including HO endonuclease and nhp6a∆, a high-mobility group box protein mutant [19]. Very high levels of Path-RIND-EDSBs were observed in both strains possessing low levels of Phy-RIND-EDSBs after treatment with caffeine, a DSB repair inhibitor. The new Path-RIND-EDSBs were not in the same location as Phy-RIND-EDSBs. Therefore, similar to DNA methylation, Phy-RIND-EDSB stabilizes the genome far beyond the Phy-RIND-EDSB complex (Figure 4). These experiments led to my conclusion that the role of Phy-RIND-EDSBs is similar to that of EDSBs induced by topoisomerase, which is DNA torsion prevention and DNA tension reduction from DNA spinning due to any DNA activity, including transcription, replication, and repair. The role of Phy-RIND-EDSBs can be imagined as gaps in a railroad track that prevent track torsion from track expansion by heat. Phy-RIND-EDSB levels decreased in chronologically aging yeast, and the reduction was directly correlated with reduced cell viability. Therefore, Phy-RIND-EDSBs play a Youth-DNA-GAPs role in preventing Path-RIND-EDSBs and DNA damage lesions [19]. Moreover, the nhp6a gene is known to prevent other types of DNA lesions, such as pyrimidine dimers [75]. Therefore, it is reasonable to hypothesize that the scatter distribution of the Phy-RIND-EDSB complex prevents all kinds of DNA damage along the length of the whole genome (Figure 4).
3.7 DNA repair activity may be compromised in aging cells by a reduction in Phy-RIND-EDSBs
A reduction in Phy-RIND-EDSBs during chronological aging may be a cause of DNA repair defects in the elderly. DNA repair machinery is known to be compromised and error-prone with age [59, 75]. Numerous studies have found a significant decline in all commonly known repair pathway activities with aging, including double-strand break repair activities [53, 54, 55, 56]. We demonstrated that the reduction in the Phy-RIND-EDSB complex will increase the production of DNA damage [19]. Therefore, aging cells have to repair DNA damage more often than younger cells. As a result, more DNA repair machinery is required for older cells. Consequently, DNA repair substrates are consumed more quickly than they are produced, resulting in DNA repair defects in the elderly.
4. Conclusion
All evidences described in this chapter suggest that genomic instability in the elderly is a vicious cycle of interactive networks among DNA damage, DNA repair, DNA demethylation, and reduction in Youth-DNA-GAPs (Figure 5). DNA damage occurs spontaneously. Then, the DNA repair process, in addition to repairing DNA damage, has consequences of reducing epigenetic marks. While NER demethylates DNA, the DSB repair pathway will repair Phy-RIND-EDSBs. DNA demethylation results in global hypomethylation and consequently reduces the homing of Phy-RIND-EDSBs. The depletion of the Phy-RIND-EDSB complex will then augment DNA damage production. Cells need to use many DNA repair substrates to eliminate DNA damage faster than these substrates are produced and eventually lose the capability of DNA repair. As a result, aging cells continue to accumulate DNA damage and send DDR signals, halting the cell cycle, causing metabolic rewiring, and eventually driving cells to enter senescence.
Figure 5.
Destructive network of aging DNA. DNA damage can occur spontaneously. The base modification repair consequence is DNA demethylation, and DSB repair for pathologic DSB will also globally repair Phy-RIND-EDSBs. Continuous DNA demethylation results in genome-wide hypomethylation, which, together with global Phy-RIND-EDSB repair, reduces the Phy-RIND-EDSB complex. A reduction in the Phy-RIND-EDSB complex augments DNA damage, and a large amount of DNA damage requires extensive DDR. Cells extensively use DDR until the DNA repair machinery is exhausted and defective, at which point, DNA damage accumulates and DDR arrests and ages cells.
Acknowledgments
All studies in Thailand were supported by the National Science and Technology Development Agency, Thailand Research Fund, and Chulalongkorn University, Thailand. I thank Dr. Maturada Patchsung, Ms Papitchaya Watcharanurak, and Ms. Sirapat Settayanon for illustration design.
Conflict of interest
The author declares no conflict of interest.
Abbreviation list
NCDs
noncommunicable disease
DDR
DNA damage repair signal
EDSB
endogenous DNA double-strand break
RIND-EDSB
replication-independent endogenous DNA double-strand break
Phy-RIND-EDSBs
physiologic RIND-EDSBs
Youth-DNA-GAPs
youth-associated genomic-stabilizing DNA gaps
IRSs
interspersed repetitive sequences
Alu
Alu elements
LINE-1s
long interspersed element-1s
HERVs
human endogenous retroviruses
BER
base excision repair
NER
nucleotide excision repair
MMR
mismatch repair
8-OHdG
8-hydroxy-2′-deoxyguanosine
AP sites
apurinic/apyrimidinic sites
DNMT
DNA methyltransferase
Alu siRNA
Alu small interfering RNA
LMPCR
ligation-mediated PCR
HMW DNA
high-molecular-weight DNA
IRS-EDSB-LMPCR
IRS-EDSB ligation-mediated PCR
HMGB
high-mobility group box
SIRT1
sirtuin 1
Path-RIND-EDSBs
pathologic RIND-EDSBs
HO
homothallic switching
OR
odds ratio
MIBs
modified ends with insertion at the breaks
\n',keywords:"genome-wide hypomethylation, genomic instability, global hypomethylation, DNA damage, youth-associated genomic-stabilizing DNA gaps, youth-DNA-GAPs, physiological replication-independent endogenous DNA double-strand breaks, RIND-EDSBs, Phy-RIND-EDSBs, aging",chapterPDFUrl:"https://cdn.intechopen.com/pdfs/65059.pdf",chapterXML:"https://mts.intechopen.com/source/xml/65059.xml",downloadPdfUrl:"/chapter/pdf-download/65059",previewPdfUrl:"/chapter/pdf-preview/65059",totalDownloads:61,totalViews:0,totalCrossrefCites:0,dateSubmitted:"September 20th 2018",dateReviewed:"December 7th 2018",datePrePublished:"December 31st 2018",datePublished:null,readingETA:"0",abstract:"Epigenetic changes are how the DNA of elderly people is prone to damage. One role of DNA methylation is to prevent DNA damage. In the elderly and those with aging-associated noncommunicable diseases (NCDs), DNA shows reduced methylation; consequently, the aging genome is unstable and accumulates DNA damage. While the DNA damage response (DDR) of the direct intracellular machinery repairs DNA lesions, too much DDR halts cell proliferation, and promotes senescence. Therefore, genome-wide hypomethylation drives genomic instability, causing aging-associated disease phenotypes. However, the mechanism is unknown. Independent of DNA replication, the eukaryotic genome retains a certain amount of endogenous DNA double-strand breaks (EDSBs), called physiologic replication-independent EDSBs (Phy-RIND-EDSBs), that possess physiological function. Phy-RIND-EDSBs are reduced in aging yeast, and low levels of Phy-RIND-EDSBs decrease cell viability and increase DNA damage. Thus, Phy-RIND-EDSBs have a biological role as youth-associated genomic-stabilizing DNA gaps. In humans, Phy-RIND-EDSBs are located in the hypermethylated genome. Because the genomes of aging people are hypomethylated, the elderly should also have a low level of Phy-RIND-EDSBs. Based on this evidence, I hypothesize that in the human Phy-RIND-EDSBs, reduction is a molecular process that mediates the genome-wide hypomethylation driving genomic instability, which is a nidus pathogenesis mechanism of human body deterioration in aging-associated NCDs.",reviewType:"peer-reviewed",bibtexUrl:"/chapter/bibtex/65059",risUrl:"/chapter/ris/65059",signatures:"Apiwat Mutirangura",book:{id:"7995",title:"Epigenetics",subtitle:null,fullTitle:"Epigenetics",slug:null,publishedDate:null,bookSignature:"Prof. Rosaria Meccariello",coverURL:"https://cdn.intechopen.com/books/images_new/7995.jpg",licenceType:"CC BY 3.0",editedByType:null,editors:[{id:"143980",title:"Prof.",name:"Rosaria",middleName:null,surname:"Meccariello",slug:"rosaria-meccariello",fullName:"Rosaria Meccariello"}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"}},authors:null,sections:[{id:"sec_1",title:"1. Introduction",level:"1"},{id:"sec_2",title:"2. Genome-wide hypomethylation",level:"1"},{id:"sec_2_2",title:"2.1 Interspersed repetitive sequence methylation",level:"2"},{id:"sec_3_2",title:"2.2 Alu hypomethylation in aging and NCDs",level:"2"},{id:"sec_4_2",title:"2.3 Mechanism causing global hypomethylation",level:"2"},{id:"sec_5_2",title:"2.4 Hypomethylation accumulates multiple kinds of DNA lesions",level:"2"},{id:"sec_6_2",title:"2.5 DNA lesions as a molecular pathogenesis mechanism of the aging process and NCDs",level:"2"},{id:"sec_7_2",title:"2.6 DNA methylation possesses a long-range effect in stabilizing the human genome in cis",level:"2"},{id:"sec_8_2",title:"2.7 Hypotheses: DNA methylation prevents genomic instability mechanisms",level:"2"},{id:"sec_10",title:"3. Phy-RIND-EDSBs represent epigenetic marks as youth-DNA-GAPs",level:"1"},{id:"sec_10_2",title:"3.1 IRS-EDSB ligation-mediated PCR (IRS-EDSB-LMPCR) to measure EDSBs",level:"2"},{id:"sec_11_2",title:"3.2 Phy-RIND-EDSBs are evolutionarily conserved epigenetic marks",level:"2"},{id:"sec_12_2",title:"3.3 Phy-RIND-EDSB or youth-DNA-GAP complex",level:"2"},{id:"sec_13_2",title:"3.4 Spontaneous pathologic RIND-EDSBs and modified ends with insertion at the breaks",level:"2"},{id:"sec_14_2",title:"3.5 Variation in RIND-EDSB level and reduction in Phy-RIND-EDSBs in aging and hypomethylated cells",level:"2"},{id:"sec_15_2",title:"3.6 Reduction in Phy-RIND-EDSBs augments pathologic EDSB production",level:"2"},{id:"sec_16_2",title:"3.7 DNA repair activity may be compromised in aging cells by a reduction in Phy-RIND-EDSBs",level:"2"},{id:"sec_18",title:"4. Conclusion",level:"1"},{id:"sec_19",title:"Acknowledgments",level:"1"},{id:"sec_19",title:"Conflict of interest",level:"1"},{id:"sec_20",title:"Abbreviation list",level:"1"}],chapterReferences:[{id:"B1",body:'Lopez-Otin C et al. The hallmarks of aging. Cell. 2013;153(6):1194-1217'},{id:"B2",body:'Hanahan D, Weinberg RA. Hallmarks of cancer: The next generation. Cell. 2011;144(5):646-674'},{id:"B3",body:'Schumacher B, Garinis GA, Hoeijmakers JH. Age to survive: DNA damage and aging. Trends in Genetics. 2008;24(2):77-85'},{id:"B4",body:'Olivieri F et al. DNA damage response (DDR) and senescence: Shuttled inflamma-miRNAs on the stage of inflamm-aging. Oncotarget. 2015;6(34):35509-35521'},{id:"B5",body:'Milic M et al. DNA damage in non-communicable diseases: A clinical and epidemiological perspective. Mutation Research. 2015;776:118-127'},{id:"B6",body:'Huang B, Jiang C, Zhang R. Epigenetics: The language of the cell? Epigenomics. 2014;6(1):73-88'},{id:"B7",body:'Mazzio EA, Soliman KF. 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Current Opinion in Genetics & Development. 1993;3(2):286-294'}],footnotes:[],contributors:[{corresp:"yes",contributorFullName:"Apiwat Mutirangura",address:"mapiwat@chula.ac.th, apiwat.mutirangura@gmail.com",affiliation:'
Department of Anatomy, Faculty of Medicine, Institution(s), Center for Excellence in Molecular Genetics of Cancer and Human Diseases, Chulalongkorn University, Bangkok, Thailand
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