Signaling pathways and transcription factors involved in valvulogenesis, osteogenesis, and CAVDa, b
Calcific Aortic Valve Disease (CAVD) occurs in >2% of the population over 65 years of age and often leads to valvular stenosis that necessitates valve replacement . CAVD is a progressive disease, often manifesting first as aortic valve sclerosis and later developing into stenosis and valve dysfunction . The specific molecular and cellular mechanisms of CAVD initiation and advancement are not well defined, and inhibitors of CAVD progression have not been identified. The current standard of treatment for CAVD is aortic valve replacement . Presently, there are no pharmacologic-based treatments for CAVD, and new therapeutic approaches for CAVD are needed. The majority of aortic valves that are replaced have congenital malformations, such as bicuspid aortic valve (BAV), establishing a link between valve development and disease mechanisms .
The molecular mechanisms of CAVD include activation of signaling pathways implicated in both heart valve development (valvulogenesis) and bone development (osteogenesis) [5-8]. These include activation of regulators of progenitor specification, cell proliferation, and differentiation. Heart valves and bone are complex connective tissues with compartmentalized ECM produced by specialized cell types. Over the past several years, extensive progress has been made in defining molecular regulatory mechanisms in heart valve and bone development (Reviewed in [8-10]). Strikingly, regulatory pathways that control development of cartilage, tendon and bone also are active in developing valves [8, 11]. In addition, recent studies have reported induction of molecular regulators of valvulogenesis and osteogenesis in CAVD [7, 12-14]. However, it is not known if these developmental mechanisms have reparative functions or contribute to the progression of CAVD.
Here we review molecular mechanisms of valve and bone development as they relate to molecular mechanisms of CAVD. Recent studies have provided evidence for the involvement of specific regulatory pathways in CAVD as activators or inhibitors of disease progression. Additional research in animal models and human patient specimens is necessary to determine the detrimental molecular regulatory pathways that promote CAVD progression and also beneficial pathways that potentially inhibit CAVD. In the future, manipulation of these pathways could be exploited therapeutically in the treatment of patients with CAVD or with aortic valve sclerosis that precedes calcification.
2. The cellular and molecular regulation of valve development
2.1. Overview of valve development
Valve development begins with the formation of endocardial cushions in the atrioventricular (AV) canal and outflow tract (OFT) of the primitive heart tube, which occurs at embryonic day (E)9-10 in mice, E3 in chickens, and E31-35 in humans . The first evidence of endocardial cushion formation is the separation of the endocardium and overlying myocardium in the AV canal by expansion of the cardiac jelly through increased expression of hyaluronan (Figure 1) . These swellings are invested with mesenchymal cells that arise from endothelial-to-mesenchymal transformation (EMT) of the endocardium . Similar swelling and induction of EMT occur approximately a day later in the cardiac OFT cushions that will contribute to the semilunar valves . Endocardial EMT is induced by signaling molecules, including bone morphogenetic proteins (BMPs), emanating from the adjacent myocardium in the AVC and OFT [8, 18-20]. Once established, the endocardial cushions expand through increased extracellular matrix (ECM) production and cell proliferation of mesenchymal and endothelial cells. The AV cushions subsequently fuse to separate right and left cardiac channels. In addition, lateral cushions are induced in the AV sulcus that will give rise to the mural leaflets of the mitral and tricuspid valves . Neural crest cells (NCCs) migrate into the cushions of the cardiac OFT, contributing to the septum between the aortic and pulmonic roots and also to the morphogenesis of individual semilunar valve leaflets [21, 22]. At this point, distinct primordia of individual valve leaflets become apparent and proliferation of valve interstitial cells (VICs) is reduced . Valve morphogenesis occurs with elongation and thinning of the valve primordia, in addition to ECM remodeling and stratification. In general, the development of the AV and semilunar (SL) valves is similar, but there are some differences in the sources of cells and structure of the resulting leaflets [8, 10, 11, 24]. In mature SL and AV valves, the ECM is stratified into collagen-rich fibrosa, proteoglycan-rich spongiosa, and elastin-rich (atrialis-ventricularis) layers oriented relative to blood flow .
2.2. Embryonic origins of valve cell lineages
The primary embryonic source of adult semilunar valve interstitial cells is the endothelial-derived cells of the endocardial cushions, that arise as a result of EMT as determined by Tie2-Cre lineage tracing in mice [23, 25]. Since the cardiac OFT is derived from the secondary heart field (SHF), semilunar VICs derived from OFT endocardium also are SHF-derived [20, 26]. NCC-derived cells are present in adult mouse semilunar valve leaflets as demonstrated by cell lineage tracing with Wnt1-Cre . These cells are predominant throughout the aortic and pulmonic valve leaflets, but are enriched in the leaflets adjacent to the aorticopulmonary septum, which also is derived from NCCs [21, 28]. NCCs are required for semilunar valve morphogenesis and remodeling, likely by providing signals necessary for cell lineage differentiation and leaflet maturation [29, 30]. Another potential source of VICs is the epicardium, which contributes cells to the parietal leaflets of AV valves . However, epicardial-derived cells (EPDCs) have not been not reported to contribute to the semilunar valves, based on Wt1-Cre fatemapping studies [31, 32]. Recent studies have reported that bone marrow-derived stem cells (BMSCs) are present in the developing and mature semilunar valves [33, 34]. Additional work is necessary to determine if these cells have lineages and functions distinct from the predominant endocardial cushion-derived or neural crest-derived VICs. It is possible that valve cell lineages derived from different developmental sources have distinct functions in normal and diseased aortic valves, but this has not yet been demonstrated. The sources of increased proliferative cells in diseased valves are relatively unknown, but could be any of these embryonic sources or, alternatively, an infiltrating cell type.
2.3. Transcription factors involved in valve development
Several transcription factors have been implicated in various processes of endocardial cushion formation and EMT (reviewed in [8, 35]). Notch pathway function in EMT is dependent on the transcription factor RBPJ, which activates expression of the bHLH transcription factor
Additional regulatory pathways control heart valve ECM remodeling and compartmentalization. Loss of NFATc1 results in defective remodeling of the AV and SL valves in mice, with embryonic lethality by E14.5 [45, 46]. EMT occurs with loss of NFATc1, but valve primordia fail to remodel and mature ECM molecules are not expressed in null mice or in cultured VICs with inhibition of receptor activator of nuclear factor κ-B ligand (RANKL) or calcineurin signaling upstream of NFATc1 activation [45, 47]. In addition to being required for endocardial cushion mesenchymal proliferation, Sox9 also promotes cartilage-like ECM gene expression in valve progenitor cells . In late stage mouse embryos, loss of Sox9 in remodeling valves results in reduced proteoglycan expression, and Sox9 haploinsufficiency in adults leads to valve calcification [40, 49]. Conversely, the bHLH transcription factor Scleraxis, critical for tendon development, promotes expression of elastic/tendon-like matrix genes in cultured valve progenitor cells . Loss of Scleraxis in mice is not lethal, but heart valve defects similar to myxomatous valve disease occur in these animals . Little is known of the transcriptional regulatory mechanisms that control the development of the valve fibrosa layer, which is most critically involved in CAVD.
2.4. Signaling pathways in valve development
Several essential embryonic signaling pathways have been implicated in endocardial cushion formation and EMT (Table 1) (reviewed in ). Transforming growth factor (TGF)β signaling was the first pathway implicated in endocardial cushion formation and is required for EMT in chicken and mouse embryonic systems (reviewed in ). BMP signaling from the myocardium is required in endothelial cells for the initiation of EMT in the AV canal, and BMP2 and 4 are the predominant ligands involved in endocardial cushion development [18-20]. Notch signaling also is required for EMT as described above. Moreover, Notch signaling is required for expression of TGFβ ligands and receptors, in addition to activating BMP signaling, which promotes mesenchymal cell invasion [36, 51]. Likewise, vascular endothelial growth factor (VEGF) signaling promotes endocardial cushion endothelial cell proliferation and EMT [47, 52]. Furthermore, targeted mutagenesis of β-catenin has implicated Wnt/β-catenin signaling in endocardial cushion EMT and mesenchymal proliferation [53, 54]. Thus multiple pathways are involved in endocardial cushion EMT and mesenchymal cell proliferation. However, the intersection and specific cellular functions of these pathways have not been fully determined.
|Role in valvulogenesis||Role in osteogenesis||Role in CAVD|
|VEGF||EMT, endothelial proliferation||angiogenesis||angiogenesis|
|Notch||EMT||Inhibit OB differentiation||represses calcification|
|TGFβ||EMT||bone homeostasis||promotes VIC calcification|
|FGF||promotes tenascin expression||OB proliferation, differentiation||blocks VIC calcification|
|BMP||EMT, PG expression||promotes OB specification||active in CAVD|
|Wnt/β-catenin||EMT, fibrosa expression||promotes OB differentiation||active in CAVD|
|RANKL||ECM remodeling||OC differentiation||promotes VIC calcification|
|Role in valvulogenesis||Role in osteogenesis||Role in CAVD|
|Twist1||ECC proliferation, migration||represses differentiation||active in CAVD|
|Msx2||EMT, proliferation||present in progenitors, OB||active in CAVD|
|Sox9||proliferation, PG expression||progenitor proliferation, cartilage differentiation||active in CAVD|
|promotes OC differentiation promotes OB differentiation||reported in CAVD|
|Runx2||not present||promotes OB differentiation||active in CAVD|
|Osterix||not present||promotes OB differentiation||reported in CAVD|
Many of the signaling pathways important for endocardial cushion formation also have later functions in valve lineage diversification, remodeling, and stratification. However, these functions have been difficult to elucidate due to limitations of available genetic tools and critical requirements for these same regulatory pathways in endocardial cushion formation. BMP signaling, as indicated by phosphorylation of the intermediate signaling molecules Smad1/5/8, is active throughout endocardial cushion mesenchymal cells, is associated with mesenchymal cell proliferation , and also is active later in valve cell lineage diversification .
The later stages of heart valve development are characterized by leaflet elongation, ECM remodeling, and stratification, all of which are critical for mature valve structure and function . Limited information is available on the regulation of these processes, but several regulatory pathways have been implicated in late valve remodeling and morphogenesis. Strikingly these same pathways have been implicated in adult CAVD (see below). RANKL, expressed by valve endothelial cells, promotes ECM remodeling and Cathepsin K (Ctsk) expression by NFATc1 in a mechanism partially conserved with osteoclast differentiation and function [11, 47, 61]. The signaling mechanisms that control stratification and ECM organization of the valve leaflets are relatively unknown. Notch signaling is localized on the ventricularis surface of the remodeling aortic valve in mice , and Wnt/β-catenin signaling is active throughout aortic valve primordia at late gestation and in a subpopulation of VICs after birth . Likewise, Wnt signaling promotes expression of fibrosa genes
Bicuspid aortic valve is arguably the most common congenital heart malformation with an incidence of 1-2% in the US adult population . BAV often does not often manifest in valve dysfunction in early life, but malformed aortic valves are predisposed to calcification. Strikingly, the majority of stenotic aortic valves that are replaced in adults are congenitally malformed . However, the molecular and cellular mechanisms of BAV are not well defined. In humans, mutations in
2.5. Extracellular matrix composition and stratification of the developing valves
The mature valve leaflets are composed of stratified ECM with layered compartments of fibrillar collagen, proteoglycan, and elastin (Figure 1) (reviewed in [10, 69]). During heart valve remodeling, there is little proliferation of VICs, but the cells are highly synthetic and produce multiple ECM proteins of the mature leaflets [24, 44]. The distinct layers of matrix are integral to heart valve function and confer specific biomechanical properties to the valve leaflets . The regulatory mechanisms for ECM remodeling and stratification are not well defined but are relevant to heart valve disease mechanisms. Periostin is required for collagen remodeling, and loss of periostin in mice leads to adult valve malformations and cardiac dysfunction [70, 71]. Likewise, mutations in
3. Molecular mechanisms of osteogenesis
3.1. Overview of skeletal development
Many osteogenic regulatory interactions identified in developing bone also are active in CAVD (Table 1). The regulatory hierarchies and ECM composition of the developing valves, most notably the collagen rich fibrosa layer, are similar to those observed in osteoblast precursor cells . Both the bone substratum and valve ECM are composed primarily of fibrillar collagen. Thus, it is not surprising that there are extensive similarities in their composition and developmental regulation. Normally, heart valves do not progress to mineralization, but striking similarities have been identified between osteogenic pathways that regulate bone mineralization and CAVD mechanisms . Thus the molecular understanding of normal development of bone has clear implications for pathogenic mechanisms of connective tissue mineralization, including CAVD.
The osteogenic precursors of the developing axial skeleton and long bones of the limbs are derived primarily from paraxial mesoderm of the developing somites and also lateral plate mesoderm, the main source of cardiac precursor cells [76, 77]. Additional progenitors of the craniofacial skeleton are derived from cranial neural crest . Most axial skeletal elements develop by endochondral bone formation that occurs through a cartilage intermediate [76, 77]. Alternatively, the craniofacial bones of the skull form through membranous ossification in which condensed osteogenic progenitors differentiate directly into bone and do not go through a cartilage intermediate . The osteochondroprogenitors present in the axial and appendicular skeletal elements develop into both bone and cartilage lineages [77, 79, 80]. Extensive research over the past several years has defined transcriptional regulatory mechanisms and signaling events that control the development of cartilage and bone (Figure 2) [79, 80].
Mature cartilage is composed predominantly of chondroitin sulfate proteoglycans that provides cushioning and flexibility to cartilaginous structures [77, 81]. In addition, the proteoglycan-rich ECM is angiostatic and mature cartilage is avascular . Interestingly, the predominant proteoglycan composition and lack of vasculature also are features of the mature aortic valve leaflet spongiosa layer . Likewise, the cartilage ECM inhibits mineralization, and a similar role has been hypothesized for the proteoglycan-rich matrix of the aortic valve . During normal axial bone development, osteoblasts from the laterally placed periostium differentiate into trabecular bone, and secondary ossification centers at the ends of the bone displace the growth plate hypertrophic cartilage [79, 80]. During bone differentiation, hypertrophic cartilage cells must die for mineralization to occur in a process of endochondral ossification, which could be related to dystrophic mechanisms of CAVD [79, 80].
Bone cell lineage maturation goes through multiple stages defined by molecular regulatory mechanisms that also are active in valve development and disease processes . Osteochondroprogenitor cells express several mesenchymal transcription factors, including Twist1, Msx2, and Sox9, that also are predominant in valve progenitor cells and diseased aortic valves . Immature pre-osteoblasts express high levels of fibrillar type 1 collagen, in addition to periostin, osteonectin, and osteopontin, similar to normal differentiated VICs [43, 60]. Differentiated osteoblasts are not yet mineralized but express the transcription factor Runx2, in addition to osteocalcin and bone sialoprotein involved in bone mineralization and also in valve calcification, [7, 80]. Later stage osteoblasts and osteocytes express the transcription factor Osterix (Osx), which is regulated by Runx2 and is required for mature bone formation . Bone mineralization occurs with the deposition of calcium phosphate and hydroxyapatite by osteocytes and is dependent on Runx2 and Osx function . Bone homeostasis is maintained throughout life by the osteogenic activity of osteocytes and bone resorption activity of osteoclasts .
3.2. Transcriptional regulation of osteoblast lineage development and bone differentiation
Twist1 is expressed early in the osteochondroprogenitor lineage and inhibits terminal differentiation of cartilage and bone . In preosteoblasts, Twist1 binds to Runx2 and inhibits its transcriptional activation of bone differentiation genes including
Sox9 functions in the expansion of cartilage progenitors and promotes cartilage differentiation, while inhibiting bone differentiation [77, 80]. Sox9 is required for osteochondroprogenitor lineage specification but is not expressed in differentiated osteoblasts . At early stages of cartilage lineage development, Sox9 promotes cell proliferation and later is required for cartilage lineage differentiation . BMP signaling induces
Runx2, originally called Cbfa1, has been defined as a master regulatory gene in bone formation [79, 93]. Gain and loss of function studies in mice demonstrate that Runx2 is both necessary and sufficient for osteoblast differentiation . During bone development, Runx2 directly regulates
NFATc1 is a critical transcription factor in osteoclast differentiation and also has been implicated in osteoblast development [80, 96]. Osteoclasts, derived from a macrophage lineage, have bone resorptive activity and are necessary for bone homeostasis . During osteoclast development, RANKL signaling induces activation of NFATc1, which promotes the transcription of bone matrix remodeling genes including C
Additional transcription factors involved in bone differentiation are not generally found in CAVD, although there are conflicting reports. Most notable is Osx, which is required for terminal differentiation of osteoblasts and mineralization of bone . Osx, promotes expression of
3.3. Signaling pathways involved in bone development
Multiple signaling pathways control the stages of bone cell lineage determination, differentiation and maturation [80, 107]. These include BMP, Wnt, and Notch pathways, also active in developing and diseased heart valves, as well as FGF, hedgehog, insulin-like growth factor (IGF), and retinoic acid (RA) pathways, not yet characterized in heart valve pathogenesis . BMP, Wnt, and Notch pathways are required at multiple stages of osteogenesis and have distinct regulatory interactions that control transcription factor function and cell type-specific gene expression in cartilage and bone cell lineages (Figure 2). In addition, these pathways crosstalk with each other in synergistic and antagonistic regulatory interactions. Strikingly many of these same regulatory interactions occur in heart valve development and pathogenesis (Table 1) .
Bone morphogenetic proteins were originally identified based on their ability to induce ectopic bone formation ; however, in vivo functions in normal bone development are less clear . In the developing limb buds, BMP signaling has a critical role in mesenchymal condensation, Sox9 activation, and cartilage lineage differentiation . Thus BMP signaling is an important regulator of the earliest stages of skeletal development. Later in differentiating osteoblasts, BMP signaling through Smad1/5/8 phosphorylation (pSmad1/5/8) promotes osteogenic differentiation and calcification . Runx2 directly binds to activated Smads1 and 5 to cooperatively activate osteoblast gene expression in response to BMP signaling . Conditional loss of BMP2 and BMP4 in the osteoblast lineage in mice inhibits late stage differentiation into Osx1-positive osteocytes, and BMP signaling is required for bone homeostasis after birth [80, 109]. Surprisingly, earlier stages of bone lineage development are apparently unaffected with conditional loss of these ligands.
Wnt/β-catenin signaling is required for osteoblast differentiation as demonstrated by loss of osteoblast differentiation with conditional loss of β-catenin in osteochondroprogenitor cells in mice . In addition, loss of β-catenin in pre-osteoblasts leads to ectopic cartilage formation, thus implicating Wnt signaling in osteogenic versus chondrogenic cell fate determination. At a molecular level, Wnt/β-catenin signaling promotes osteoblast lineage differentiation, while inhibiting chondrogenesis, by activating Runx2, while inhibiting Sox9 . In bone lineages, BMP and Wnt signaling act synergistically to promote calcification, although neither pathway alone is sufficient to induce a full osteogenic response . During the initial differentiation of bone progenitor cells, regulatory elements of
Notch activation inhibits osteogenesis through suppression of the Wnt/β-catenin pathway and Runx2 transcription factor activity [94, 113, 114]. Loss of Notch1 or Notch2 function promotes osteoblast differentiation and leads to increased bone mass in mice . Notch pathway activation inhibits the progression of osteoblast differentiation through direct binding of the activated Notch1 intracellular domain (N1ICD) to β-catenin, thereby counteracting Wnt-mediated induction of osteogenesis [113, 114]. In addition, the Notch target gene
4. Molecular and cellular mechanisms of CAVD
4.1. Overview of CAVD progression
The mature aortic valves are comprised of three ECM layers critical for normal leaflet structure and function [24, 44, 117]. Collagen predominates in the fibrosa layer, which is oriented on the opposite side of blood flow, whereas elastin is enriched in the ventricularis layer on the flow side of the valve. Between the fibrosa and ventricularis layers, is the proteoglycan-rich spongiosa layer [24, 44, 117]. This trilaminar ECM arrangement is preserved among species, and lends both strength and elasticity to the aortic valves . In CAVD, the aortic valve becomes thickened and displays extensive ECM remodeling and mineralization [118-121]. Abnormal thickening (aortic valve sclerosis) and calcification of the aortic valve lead to stiffening of the valve leaflets and can reduce the effective valve opening (aortic valve stenosis), which can impede blood flow and lead to clinical symptoms such as syncope and angina [119, 122, 123]. Histologically, human explanted diseased aortic valves have extensive ECM remodeling and elastic fiber fragmentation with evidence of both macroscopic calcific nodule formation as well as microscopic mineral deposits .
Changes in the resident VICs are apparent in CAVD. Under normal conditions, aortic VICs are quiescent and non-proliferative [13, 24, 104, 124]. However, in disease, a subset of aortic VICs exhibits features of myofibroblast activation, which is characterized by expression of α-smooth muscle actin (αSMA), MMP13, non-muscle myosin heavy chain (SMemb), and markers of proliferation [13, 104, 119, 124, 125]. In vivo, the factors responsible for inducing myofibroblast activation are not well defined. However, in culture, TGFβ1 stimulation and mechanical strain are potent inducers of VIC myofibroblast activation [125, 126]. Activated VICs also exhibit characteristics of valve and bone precursor cells as they induce expression of the common mesenchymal markers Sox9, Twist1, and Msx2 . Currently it is unknown where the mesenchymal-like cells come from and what role these proliferative cells play in the progression of CAVD pathogenesis.
Valve calcification, apparent as hydroxyapatite deposits on the surface of or within the leaflets, is a prominent feature of CAVD [119, 127, 128]. Histologically, valve calcific nodules are primarily acellular [13, 129]. Although traditionally thought to be a completely passive deposition of mineral, in some cases, valve calcification is coincident with endochondral bone-like and cartilaginous-like nodules [129, 130]. Aortic valve calcification is observed primarily in the regions of the valves exposed to the greatest physical strain, specifically at the hinge region of the valve and along the line of leaflet coaptation . Furthermore, calcification is predominantly found in the fibrosa layer of the diseased valve, which is similar to early bone matrix as it is contains primarily fibrillar collagen . Expression of other bone matrix molecules, such as osteocalcin and osteopontin, are induced during disease . Furthermore, expression of osteogenic factors, such as Runx2, BMP2, and alkaline phosphatase, also is induced in VICs from calcified valves, suggesting that resident VICs may have the potential to undergo osteogenic transdifferentiation and actively contribute to valve calcification (reviewed in ).
Extrinsic factors have been implicated in valve calcification. For example, lipid deposition and immune cell infiltration are common histopathological features of CAVD, and it has been proposed that aortic valve calcification occurs by mechanisms similar to arterial calcification in atherosclerosis [119, 132-135]. In addition, altered external physical forces elicit changes in resident VICs, which play an active role in pathological valve calcification . In contrast to VIC response to immune cell infiltration and altered physical forces, cell intrinsic mechanisms may also contribute to valve calcification, as stimulation with factors such as BMP2 or TGFβ1 in cell culture studies can induce VIC calcification in the absence of inflammatory stimulation or altered physical forces [126, 136-138]. Together, these studies suggest that not only is valve calcification an active cell-regulated process, but that many factors likely contribute to progression of calcification during disease. It is also likely that not all CAVD is created equal. Genetic predisposition, the presence of a malformed aortic valve, and other disease comorbidities, such as coronary artery disease, hypertension, and kidney disease, likely affect the pathology and underlying cause of CAVD [64, 139-142].
4.2. Activation of progenitor cell and osteogenesis-related molecular pathways in CAVD
4.2.1. Expression of valve and bone progenitor cell genes in CAVD
The mesenchymal markers Twist1, Msx2, and Sox9 are expressed in adult calcific aortic valves in mesenchymal-like activated VICs [12, 13, 106, 143, 144]. As discussed, these genes are expressed in both valve and bone mesenchymal progenitor cells. A recent study has compared gene expression in pediatric versus adult aortic valve disease and shown that the mesenchymal markers Twist1, Msx2, and Sox9 are increased in both . The observation that both pediatric and adult diseased valves have increased expression of the mesenchymal markers suggests that this expression is related to VIC activation and proliferation, which is common to both, and not related to valve calcification, which is found only in advanced adult disease . In both valve and bone progenitors, Twist1, Msx2, and Sox9 induce proliferation and promote a mesenchymal phenotype, thus reactivation in diseased valves is suggestive of a similar role in valve pathogenesis [38, 40-42, 84, 88, 145]. Although it is presumed that resident VICs re-activate these early mesenchymal markers, other possibilities exist. EMT as a mechanism for VIC activation has not been established in CAVD, however, recent studies report EMT-like events in adult valves. Increased cyclic strain and altered hemodynamics, both recognized features of CAVD, can induce EMT in isolated sheep valve endothelial cells [146, 147]. In addition, cultured valve endothelial cells stimulated with TGFβ adapt a mesenchymal-like phenotype and express markers of both endothelial and mesenchymal cells, suggesting that they can undergo EMT [148, 149]. Likewise, disruption of Notch signaling in adult mice induces aortic valve thickening with evidence of endocardial EMT, as indicated by endocardial cells with more pseudopodial projections, loose endocardial cell-cell junctions, and αSMA expression . Additional sources of mesenchymal-like cells have been suggested. For example, circulating bone marrow-derived hematopoietic stem cells have been shown to integrate into the valve interstitium, adapt fibroblast-like characteristics, and surround regions of prominent valve calcification in human end stage CAVD [33, 130, 151]. It is uncertain what role reactivation of the mesenchymal markers Twist1, Msx2, and Sox9 have in potential valve repair mechanisms or in the progression of CAVD. It is possible that adult VICs maintain a certain “mesenchymal-plasticity” and are able to revert back to an early progenitor-like mesenchymal cell during disease. Alternatively, they may be indicators of newly derived VICs arising from EMT or circulating progenitor cell populations in response to disease conditions.
4.2.2. Osteogenic factors in CAVD
Molecular mechanisms of endochondral ossification and cartilaginous nodule formation are active in CAVD [7, 13]. Studies in human explanted diseased aortic valve tissues have demonstrated increased expression of the osteogenic factors BMP2, TGFβ1, Runx2, osteocalcin (OCN), osteopontin (OPN), osteoprotegerin (OPG), bone sialoprotein (BSP), alkaline phosphatase (ALP), and Osx in disease [5, 13, 106, 118, 137, 152]. At a molecular level, BMP2 signaling is a key inducer of VIC calcification, which is thought to act through p-SMAD1/5/8 and phospho-ERK1/2 signaling to stimulate increases in both Runx2 and OPN expression . Induction of VIC calcification by BMP2 stimulation is highly reminiscent of BMP signaling in bone development, suggesting that some parallels exist between osteogenic bone formation and VIC calcification . Histological studies of explanted human valves further support a role for BMP signaling in valve calcification. Comparison of pediatric diseased valves, which do not acquire calcification, and adult calcified valves demonstrates that increased BMP signaling, evident in p-SMAD1/5/8 activation, is exclusive to adult valves with calcification, indicating that BMP signaling may contribute to valve calcification in human disease . Additionally, TGFβ1 is also a potent inducer of osteogenic-like differentiation of VICs in cell culture, as it stimulates VIC activation and calcification, increases ALP activity, and increases expression of ECM remodeling enzymes [126, 136, 137]. Negative regulators of valve calcification have been demonstrated through in vivo studies. One negative regulator of valve calcification is Notch signaling. Animals haploinsufficient for Notch signaling develop aortic valve calcification with increased BMP signaling and increased expression of Runx2 in the valve leaflets [65, 153]. Studies in isolated aortic VICs further demonstrate that Notch signaling plays an important role in suppressing valve calcification as treatment of VICs with Notch inhibitors induces BMP signaling and subsequent increases in osteogenic gene expression [65, 153]. Another negative regulator of valve calcification is Sox9, which potentially acts through induction of proteoglycan expression, similar to what has been observed in developing cartilage [40, 49]. Conditional heterozygous Sox9 mutant mice develop valve calcification along with increased valve thickness and expression of the osteogenic genes
CAVD has been linked to chronic kidney disease in human patients and animal models [140, 154-157]. A prominent pathological feature of kidney disease is the inability to regulate calcium and phosphate metabolism . Increased blood phosphate levels (hyperphosphatemia) are highly associated with aortic valve sclerosis and valve calcification in humans . Klotho-null mice are a model of accelerated aging that includes development of kidney failure and hyperphosphatemia, along with cardiovascular disease [159-162]. Klotho-null mice exhibit extensive valve annulus calcification with increased expression of osteogenic genes, but minimal CD68 positive macrophage infiltration . Thus, valve calcification in the klotho-null animals parallels bone formation, where increases in crucial osteogenic genes, such as
Atherogenic lipid deposition and inflammation in the valves also has been linked to induction of osteogenic gene expression and disease [163-165]. Rabbit and mouse models of CAVD, induced with hypercholesterolemic or high fat diets, have increased lipid deposition and macrophage infiltration associated with induction of osteogenic markers such as ALP, OCN, OPN, Runx2, and Osx [165-167]. Although osteogenic gene expression is induced in these models, this type of valve calcification closely mimics vascular calcification observed in atherosclerosis, rather than endochondral bone formation, due to the presence of extensive immune cell infiltration [165, 166]. In support, human aortic VICs stimulated with pro-inflammatory mimetics not only induce the expression of inflammatory cytokines, but also induce the expression of osteogenic factors, such as BMP2 and Runx2, again suggesting that this process may be similar to what is occurring in atherosclerotic disease [164, 168]. Based on this evidence, multiple physiologic factors likely contribute to osteogenic gene induction in calcified diseased aortic valves.
4.2.3. Valvulogenic- and osteogenic-related signaling pathways in CAVD
As in both heart valve and endochondral bone development, BMP, TGFβ, Notch, and Wnt signaling have been implicated in the progression of CAVD (Figure 2; Table 1). Increased BMP ligand expression, particularly BMP2 and BMP4, has been demonstrated histologically in human explanted calcific aortic valves surrounding and throughout regions of valvular calcification [118, 129]. Furthermore, active BMP signaling, as indicated by pSMAD1/5/8 expression, is present in both human explanted diseased aortic valves and animal models of CAVD [13, 169, 170]. Comparison of pediatric diseased valves void of calcification to heavily calcified adult diseased valves demonstrates extensive ECM remodeling and evidence of VIC activation in both; however, increased pSMAD1/5/8 signaling is exclusive to calcified valves . The observation that pSMAD1/5/8 expression is found only in adult calcified valves is suggestive of a critical role for BMP signaling as an initiating osteogenic factor in CAVD . Furthermore, increased pSMAD1/5/8 expression reportedly localizes to the fibrosa layer of human calcific aortic valves, which is the primary sight of aortic valve calcification . Cell culture studies support this and show that BMP2 stimulation promotes osteogenic-like aortic valve calcification in human aortic VICs by inducing the expression of the osteogenic factors Runx2, OPN, and ALP [138, 171]. Based on this evidence, active BMP signaling may be a potential therapeutic target to treat CAVD, however it has not yet been tested.
TGFβ signaling induces α SMA expression and myofibroblast differentiation of porcine aortic VICs, suggesting that TGFβ promotes VIC activation, potentially in response to physical strain [125, 172]. Furthermore, TGFβ signaling may also have a role in aortic valve calcification, as human explanted calcific aortic valves have increased levels of TGFβ1 expression and ovine aortic VICs in culture calcify in response to TGFβ1 induction [136, 137]. TGFβ signaling has also been linked to both Wnt/β-catenin and FGF signaling pathways in CAVD [173, 174]. Specifically, FGF signals have been shown to induce MAPK signaling, which inhibits aortic VIC αSMA expression and myofibroblast response to TGFβ . In addition, TGFβ stimulation of aortic VICs induces nuclear localization and activation of β-catenin, which promotes VIC myofibroblast differentiation . Although the role of TGFβ in CAVD is not well established in vivo, there is accumulating evidence for a role in VIC activation and calcification from studies in cell culture systems.
Whereas both BMP and TGFβ signaling have been found to induce VIC calcification, Notch signaling has been implicated as a negative regulator of valve calcification. Familial studies demonstrated that Notch1 haploinsufficiency is associated with CAVD and aortic stenosis (AS) . During development, Notch1 is expressed in the endothelial cells lining the aortic valve cusps and is also observed at lower levels in the VICs, and this expression pattern is maintained into adulthood [64, 175]. Histological analysis of human explanted aortic valves demonstrates that activated Notch1 intracellular domain (NICD) expression is dramatically reduced in VICs directly adjacent to regions of aortic valve calcification . This observation is consistent with a mechanism whereby Notch signaling inhibits valve calcification and downregulation of Notch expression promotes valve calcification . The idea that Notch signaling functions as a negative regulator of calcification was originally defined in endochondral bone formation, where downstream effectors of Notch signaling, Hes1 and Hey1, repress Runx2 transcriptional function, leading to expansion of hypertrophic cartilage and impaired osteoblast differentiation . Notch1 heterozygous or RBPJ heterozygous mice develop CAVD, as evidenced by increased aortic valve calcification, and also display significant increases in BMP/pSMAD1/5/8 signaling and Runx2 expression in the aortic valves [65, 153]. Likewise, deletion of RBPJ in adult mice results in increased aortic valve thickness with evidence of VIC proliferation and potentially, endothelial EMT . Together these in vivo studies support the idea that Notch signaling represses BMP expression, thereby indirectly repressing other osteogenic factors [65, 153]. Cell culture studies indicate that Notch inhibition promotes calcification of VICs by repressing chondrogenic genes, including
Wnt/β-catenin signaling is important for osteoblast maturation during embryonic development and contributes to mineralized bone formation (reviewed in ). A number of studies have also shown activation of Wnt/β-catenin signaling in aortic valve calcification. Canonical Wnt signaling acts through the frizzled receptors and the Wnt co-receptors Lrp5 and Lrp6, resulting in β-catenin nuclear localization and TCF/LEF1 activation . Human explanted calcific AoVs have increased expression of Lrp5, β-catenin, and Wnt3a ligand as compared to control valves . Increased Wnt signaling in diseased aortic valves also has been observed in multiple animal models of CAVD. Pigs and rabbits maintained on an atherogenic diet develop aortic valve disease and display increased expression levels of β-catenin and Lrp5 receptor [173, 177]. Likewise, in a subset of endothelial nitric oxide synthase (eNOS) deficient mice that develop BAV, expression of Wnt3a ligand and Lrp5 receptor is increased when the animals are fed a high cholesterol diet . Cell culture studies also support the idea that Wnt/β-catenin signaling is important for VIC myofibroblast activation, proliferation, and chondrogenic gene induction. Studies in porcine aortic VICs show that Wnt3a treatment induces significant VIC proliferation and myofibroblast activation [173, 179]. Furthermore, Wnt3a treatment of embryonic chicken aortic VICs results in increased expression of
4.3. Matrix remodeling in CAVD
Diseased aortic valves are characterized by changes in the ECM; in particular, disorganized collagen bundles and extensive elastic fiber fragmentation are observed [181, 182]. Insight into the role of elastin fiber disorganization in the pathogenesis of CAVD has been provided through studies of elastin haploinsufficient mice, which display elastin fiber fragmentation, abnormal ECM remodeling, and increased valve stiffness, suggesting that elastin homeostasis is important for maintaining valve function [72, 183]. Collagen synthesis and remodeling are dramatically increased in CAVD, however, overall collagen content in the valve is actually decreased, suggesting that there is extensive collagen proteolysis during disease [184-186]. In contrast to collagens, expression of proteoglycans, including decorin, biglycan, versican, and hyaluronan, is increased particularly in regions of the diseased valve adjacent to calcific nodules . These changes in ECM composition during CAVD can be compared to matrix remodeling events that occur during valve development and also in bone formation. The decreased collagen content and increased proteoglycan matrix found in CAVD is similar to the primitive ECM characteristic of early valve development . Furthermore, parallels can also be drawn between matrix remodeling in CAVD and bone development. Specifically, matrix remodeling in the immature bone is essential for providing a scaffold upon which the calcified matrix is deposited, and subsequent ECM degradation is essential for expansion of the calcified regions of newly forming bone . The parallels between matrix remodeling in bone development and the disease process of CAVD suggest that valve matrix remodeling may contribute to valvular calcification.
Matrix degradation and remodeling in valvulogenesis, osteogenesis, and CAVD occurs concomitant with increased activity of MMPs and cathepsins, along with increased RANKL signaling. A number of studies have shown significant increases in expression of multiple MMPs, including MMP1, MMP3, MMP7, MMP9, and MMP12, with increased cathepsins B, K, and S in human calcific diseased aortic valves, suggesting that extensive ECM remodeling is a key feature of disease [124, 163, 181, 182, 184, 190]. In bone, RANKL signals through the RANK receptor, which can be inhibited via binding to the soluble receptor OPG, and promotes the expression of proteolytic enzymes, such as MMPs and cathepsin K, through activation of NFATc1 [191, 192]. A similar mechanism has been identified in heart valve remodeling [47, 61]. Comparison of sclerotic diseased aortic valves and advanced stenotic aortic valves determined that OPG levels are significantly higher in sclerotic valves without calcification, whereas RANKL expression is higher in stenotic calcified valves [103, 193]. This study concluded that OPG may be protective against valve calcification, whereas elevated RANKL expression may promote valve calcification by promoting upregulation of matrix remodeling enzymes [103, 193]. Furthermore, treatment of human aortic VICs with RANKL results in increased MMP1 and MMP2 activity with increased VIC proliferation, concomitant with increased calcification and osteogenic gene expression [103, 191]. In addition, NFATc1 expression is increased in human explanted aortic valve leaflets with CAVD . Together, these studies are consistent with signaling events during bone development, namely RANKL activation of NFATc1, stimulating matrix remodeling enzymes, and promoting calcification .
A number of other signaling pathways are likely involved in ECM changes that occur during CAVD. In particular, TGFβ1 stimulation of cultured VICs stimulates myofibroblast differentiation, leading to increased levels of αSMA stress fibers in the VICs [125, 172]. It has been suggested that these myofibroblasts then exert a contractile force on the surrounding valve ECM and stimulate rearrangement of the matrix, particularly in fibronectin fibers . Furthermore, TGFβ1 stimulation also induces increased type I collagen production and expression of the matrix remodeling enzymes MMP9 and MMP2 in cultured aortic VICs [136, 172]. These studies indicate that TGFβ1 signaling may be a key factor in ECM-related changes during CAVD pathogenesis. Moreover, Wnt signaling may work in concert with TGFβ1 to induce changes in ECM during CAVD . TGFβ1 stimulation promotes nuclear localization and activation of β-catenin in cultured VICs, and, when combined, Wnt and TGFβ1 signaling dramatically increases myofibroblast activation . In contrast to TGFβ1 and Wnt signaling, FGF signaling may work to inhibit ECM remodeling during valve disease. FGF signaling has been shown to block TGFβ1 induced myofibroblast differentiation and αSMA expression in porcine aortic VICs through activation of phospho-ERK1/2 signaling . In addition, FGF signaling inhibits myofibroblast contraction of a collagen matrix, supporting the idea that FGF signaling blocks TGFβ1 stimulation of matrix-related changes . Many parallels exist between signaling factors involved in ECM changes in development and disease. In particular, RANKL, TGFβ1, Wnt, and FGF signaling have demonstrated roles in ECM production and regulation in both heart valve and endochondral bone formation [8, 76]. The shared signaling pathways in these tissues, both in development and disease, suggest that developmental pathways may be reactivated in CAVD to induce matrix changes characteristic of the disease.
5. Therapeutic mechanisms in CAVD
Currently, aortic valve replacement surgery is the only effective treatment option for CAVD . There have been numerous studies, which are summarized below, testing the effectiveness of different pharmacotherapies on preventing the progression of AS. Unfortunately, studies on statin therapies, inhibitors of the renin-angiotensin-aldosterone system, and osteoporosis treatments have not been proven to be effective at preventing the symptoms or the progression of CAVD/AS. Following the summary of these studies, additional treatment options, related to the expression of developmental and osteogenic-related genes in CAVD, are discussed.
Lipid deposition and the accumulation of apolipoproteins (Apo) in the aortic valve leaflets have long been associated with CAVD, and many studies have compared the progression of CAVD to atherosclerotic disease [119, 133, 134, 194]. Therefore, it has been hypothesized that cholesterol lowering therapy with statin drugs may be an effective treatment strategy to delay the progression of CAVD. A specialized mouse model called “Reversa” mice develop signs of CAVD when fed a high cholesterol diet, however, when serum cholesterol is lowered via a genetic deletion of the microsomal triglyceride transfer protein (Mttp), reduced levels of aortic valve calcification, as well as decreased expression of the osteogenic markers pSMAD1/5/8, Msx2, Osx, β-catenin, and Runx2, are observed [167, 170]. Thus reducing plasma cholesterol may reduce CAVD pathogenesis, particularly in terms of reducing osteogenic gene expression in the diseased valves. Similarly, statin treatment of human or porcine aortic VICs cultured concomitantly with osteogenic media results in decreased expression of the osteogenic genes ALP, OCN, Lrp5, and OPN, and reduced calcific nodule formation [171, 177, 195]. However, when statin treatment of aortic VICs is initiated after osteogenic transformation or calcific nodule formation, it is ineffective at reducing calcification and expression of osteogenic markers, indicating that statin therapy cannot reverse aortic valve calcification and osteogenic differentiation once it has occurred [195, 196]. Results from animal studies are equally contradictory. Rabbits fed a high cholesterol diet supplemented with atorvastatin have decreased aortic valve thickness, reduced VIC proliferation, and reduced expression of Lrp5, β -catenin, OPN,
Clinicial studies investigating the use of statin therapy in patients with CAVD are also widely contradictory. An early study investigating the use of statin therapy in patients with moderate to severe aortic stenosis (AS) reported that patients treated with statins had less hemodynamic progression of AS over a 2 year time period than patients who were not on statin therapy . In contrast, three larger prospective clinical studies, SALTIRE (Scottish Aortic Stenosis and Lipid Lowering Trial, Impact on Regression), SEAS (Simvastatin and Ezetimibe in Aortic Stenosis), and ASTRONOMER (Aortic Stenosis Progression Observation: Measuring Effects of Rosuvastatin), found that statin therapy was not effective at treating the progression of AS [199-201]. In these trials, three different statin therapies were investigated in patients with mild to moderate AS and it was determined that statin therapy did not alter the progression of CAVD/AS nor prevent outcomes such as the necessity to undergo aortic valve replacement surgery [199-201]. In response to the negative outcomes of these large clinical trials, the use of statin therapy was next investigated in patients with the earliest form of CAVD/AS, aortic valve sclerosis, to determine if statin use could prevent, rather than reverse, AS . In this report, statin therapy was significantly associated with a decreased development of AS and a decreased need for aortic valve replacement surgery, suggesting that statin therapy may be an effective treatment if started at the earliest stages of the disease, prior to any indication of valve calcification . Based on these studies, it can be concluded that in humans, statin therapy is ineffective at preventing the progression of AS and reversing aortic valve calcification. However, statin therapy may be useful at preventing the onset of AS in patients with the earliest stages of aortic valve thickening.
5.2. Angiotensin converting enzyme inhibitors/Angiotensin receptor blockers
Another potential therapy to prevent the progression of CAVD/AS is the use of the anti-hypertensive angiotensin-converting enzyme inhibitors (ACEI) and angiotensin receptor blockers (ARB). Currently, ACEIs and ARBs are prescribed to treat hypertension, and function by acting on the renin-angiotensin-aldosterone system to ultimately inhibit the vasoconstrictor effects of angiotensin II . Previous reports have identified the overlapping expression of angiotensin-converting enzyme (ACE) and angiotensin II in calcified human aortic valves surrounding regions of valvular calcification . It has been hypothesized that ACE inhibition may prevent the progression of CAVD by reducing ACE activity in the diseased valve leaflet [204-206]. A study conducted in ApoE knockout mice with induced chronic renal failure concluded that animals treated with the ACEI enalapril had significantly reduced levels of pathologic aortic valve leaflet thickening and valve fibrosis than untreated animals . Similarly, in a rabbit model of CAVD in which the animals were fed a high vitamin D diet, treatment with the ACEI ramipril significantly reduced the progression to AS, improved valve endothelial cell integrity, and reduced aortic valve calcification . It is uncertain how ACE and angiotensin receptor (AR) inhibition would directly affect molecular changes in the valve leaflets. However, in a study of rabbits fed a high cholesterol diet, treatment with the ARB olmesartan decreased the number of α SMA positive myofibroblasts and reduced expression of the osteogenic markers
Clinical studies testing the therapeutic benefits of ACEIs and ARBs in CAVD progression have had mixed results. In human explanted aortic valve tissues, ARB therapy is associated with reduced aortic valve remodeling and calcification . As in animal studies, this histological analysis suggests that AR inhibition may limit aortic valve calcification . In a small pilot clinical study (Symptomatic Cardiac Obstruction – Pilot Study of Enalapril in Aortic Stenosis), use of the ACEI Enalapril was associated with improved clinical symptoms in patients with severe symptomatic AS . The majority of studies investigating the use of ACEIs or ARBs in CAVD/AS with positive outcomes have been retrospective. In three different retrospective studies, ACEI or ARB use in patients with mild to moderate AS was associated with decreased mortality, decreased number of adverse cardiovascular events, slower progression of AS, and less accumulation of valvular calcification [209-211]. Additionally, one prospective study followed a small random population of patients over a 4-year period and reported that the use of ACEIs or ARBs was significantly associated with reduced CAVD/AS disease progression . Together, these studies provide evidence that ACEI and ARB may delay CAVD progression. Conversely, there have also been a number of studies that show no association between ACEI and ARB use and improved outcomes in CAVD progression. The JASS study (Japanese Aortic Stenosis Study) reported that ARB therapy in patients with moderate to severe AS had no beneficial outcomes in CAVD progression, although patients with mild asymptomatic AS had some indication of reduced progression to AS . Furthermore, a large study in patients with very mild asymptomatic AS found that patients on ACEI or ARB therapy had no improvement in the progression of AS compared against a control group . Similarly, a small 2-year study observed no difference in the hemodynamic progression of AS with ACEI use versus non-use . Based on both animal and clinical studies, it is unclear whether ACEI or ARB therapy is an effective treatment option to prevent the progression of CAVD/AS, however there are indications that perhaps this therapy may limit valve calcification [204, 207]. A placebo controlled, blinded trial will be necessary to determine the effectiveness of these therapies in treating CAVD.
5.3. Aldosterone-receptor antagonists
Aldosterone is a component of the renin-angiotensin-aldosterone system that plays a key role in the kidney to regulate water and sodium reabsorption and effectively raise blood pressure . Aldosterone-receptor antagonists (ARA) are commonly prescribed for their diuretic effects . Recently, there have been two studies investigating the use of ARAs in the treatment of CAVD/AS. In an animal study, rabbits fed a high cholesterol diet develop aortic valve sclerosis, with thickening of the valve leaflets and microscopic calcific deposits, which was blocked by treatment with the ARA eplerenone . In addition to reducing valve fibrosis and mineralization, evidence of macrophage infiltration was also reduced . Conversely, in a small placebo-controlled human trial of patients with moderate to severe asymptomatic AS, there was no difference in the progression of AS in those patients receiving the ARA eplerenone versus placebo . To our knowledge, no molecular evidence has been reported in studies on ARA therapy in CAVD and it is unknown whether ARA therapy affects myofibroblast activation or osteogenic gene induction. Additional clinical studies will be necessary to determine if ARA use can prevent AS progression if therapy is started in early disease stages.
Bisphosphonates (BP) are a class of drugs that mimic inorganic pyrophosphate and prevent ectopic soft tissue calcification and inhibit bone resorption . In adults, especially women, BPs are commonly prescribed to treat excessive bone resorption associated with osteoporosis . Human aortic VICs, grown on collagen gels in the presence of a specialized thiol bisphosphonate, have decreased ALP activity and reduced cellular aggregation, a step that precedes calcific nodule formation, as compared to cells grown on collagen alone . This study suggests that bisphosphonates may inhibit VIC calcification in vitro and could serve as a potential therapeutic strategy to prevent aortic valve calcification . Due to the ability of BPs to prevent ectopic calcification in bone and the availability of patient populations currently using BPs, a number of studies have investigated the use of BPs in the inhibition of aortic valve calcification. Three small retrospective human studies compared measurements of AS progression over a 2-year period in patients with AS taking BPs versus those not taking BPs [218-220]. The results of these studies suggest a modest reduction in the progression of AS in those patients taking BPs [218-220]. The large MESA study (Multi-Ethnic Study of Atherosclerosis) followed women taking BPs, compared to those not taking BPs, and their development of CAVD/AS over time . The results of this study were mixed and showed that, in older women, BP therapy was associated with a slight benefit in terms of aortic valve calcification, whereas, younger women taking BPs had significantly more progression of aortic valve calcification compared to women not taking BPs . Most recently, a large retrospective study investigated the progression of AS in women with mild to moderate AS over a 5-year period and compared the outcomes in patients on BP therapy versus those not taking BPs . The evidence from this study shows that there was no change in survival, or in the number of aortic valve replacement surgeries, in women taking BPs compared to those not taking BPs, suggesting that BP therapy does not suppress the progression of CAVD/AS . Thus far, the outcomes of the human studies investigating the use of bisphosphonate therapy in CAVD demonstrate that this therapy is ineffective at preventing or delaying the progression of CAVD/AS. To definitively determine whether or not BP therapy is effective at suppressing the progression of CAVD/AS, placebo-controlled prospective studies will be necessary.
5.5. Nitric oxide bioavailability
Endothelial nitric oxide synthase (eNOS) produces nitric oxide (NO) from L-arginine, and eNOS expression has been identified in the endothelial cells lining the aortic valves . eNOS deficiency has been linked to defective aortic valve development, as approximately 50% of eNOS deficient mice develop a bicuspid, rather than tricuspid, aortic valve . eNOS deficient mice with a BAV phenotype fed a high cholesterol diet develop hemodynamic symptoms of AS and also display microscopic mineralization in the aortic valve leaflets, indicating that eNOS activity may be important for suppressing aortic valve calcification . Nitric Oxide deficiency is also an indicator of endothelial cell dysfunction, and systemic endothelial cell dysfunction is prevalent in patients with aortic valve sclerosis/stenosis [223-225]. The uncoupling, or dysfunction, of eNOS results in decreased NO production and increased generation of reactive oxygen species (ROS) . ROS activity is present in calcific lesions of human stenotic aortic valves, and it has been suggested that ROS activity may speed aortic valve calcification [144, 226]. In animal studies, rabbits fed a high cholesterol/high vitamin D diet develop aortic valve thickening, small deposits of valve calcification, and increased ROS activity in cells surrounding regions of valve calcification . Furthermore, ROS activity was co-localized to clusters of cells expressing Runx2 and OPN, suggesting that ROS activity is associated with VICs displaying an osteogenic-like phenotype . In VIC culture studies, TGFβ1 stimulation induces increased ROS activity, along with calcific nodule formation and ALP activity . Increasing the availability of NO, via NO donors such as sodium nitroprusside, partially blocks both nodule formation and ALP activity, suggesting that NO levels are important for reducing ROS and inhibiting calcification in VICs . There have been a number of small clinical studies investigating the levels of the NOS inhibitor, asymmetric dimethylarginine (ADMA), an indicator of endothelial cell dysfunction, in patients with moderate to severe AS [228-230]. In these studies, plasma levels of ADMA are significantly higher in patients with moderate to severe AS, compared to patients with mild AS or no disease, suggesting that NO production is disrupted in CAVD/AS [228-230]. Combined, these studies suggest that increased ROS production is associated with aortic valve calcification and the induction of osteogenic gene expression. Thus, increasing the bioavailability of NO may be a potential therapeutic avenue to block ROS activity, and thereby disease progression, in CAVD.
5.6. NSAIDs/COX2 inhibitors
Previous reports have demonstrated that immune cell infiltration is common in CAVD [119, 132, 135]. Non-steroidal anti-inflammatory drugs (NSAIDS) are commonly used to treat pain and inflammation, and act by inhibiting the enzymes COX1 and/or COX2 [231-233]. These enzymes function by converting arachidonic acid to prostaglandins (PGs) [233, 234]. There has been one study conducted in human aortic VICs, demonstrating that stimulation of VICs with pro-inflammatory mimetics induces the expression of COX2 and the release of prostaglandins . This study suggests that COX2 inhibition may be one way to treat the immune response associated with CAVD . Interestingly, COX2 and PG signaling are also involved in bone formation as well as cellular responses to physical stress and strain, processes that also likely contribute to CAVD (reviewed in ). In bone, PG signaling has an anabolic effect, and PG treatment of osteoblast cultures results in increased expression of OCN, BMP2, Runx2, OPN, ALP, and BSP [236-244]. In osteoblast cultures, BMP2 stimulation induces COX2 expression through upregulation of Runx2, which binds to and activates the COX2 promoter [245, 246]. Downstream, PG signaling induces the expression of p38 MAPK through the activation of protein kinase A [243, 247]. Furthermore, fluid shear stress and other physical forces induce COX2 expression in osteoblast-like cells, suggesting that increased COX2 and PG signaling is a cellular response to altered mechanical forces [248, 249]. The combined results of these studies suggest that COX2/PG signaling may be an effective therapeutic target to treat CAVD progression, as COX2/PG signaling plays a role in inflammation, osteogenesis, and cellular response to physical strain, all of which are thought to be pathological mechanisms involved in CAVD [131, 243, 244, 248-251]. It would be interesting to determine whether COX2 inhibition/NSAID use could reduce CAVD progression. However, one caveat is that COX2 inhibitor therapy can be associated with some rare but significant adverse cardiovascular events such as myocardial infarction and stroke [252, 253]. Perhaps therapeutics designed toward downstream targets of PG signaling, such as p38 MAPK, could improve outcomes of CAVD patients without the cardiovascular side effects of selective coxibs .
5.7. Development of new therapeutic approaches based on valvulogenic and osteogenic molecular mechanisms.
As reviewed above, Notch, Wnt, and BMP signaling have been implicated in the progression of CAVD. Pharmacotherapies designed to act as Wnt and BMP inhibitors, or Notch agonists, could be a potential avenue for new therapeutics to treat the progression of CAVD. BMP signaling is thought to be a specific indicator of aortic valve calcification as active BMP signaling is observed in adult diseased valves with prominent calcification and is not found in pediatric diseased valves void of calcification . Furthermore, BMP2 signaling stimulates VIC calcific nodule formation and induces osteogenic gene expression [138, 171]. It is possible that therapies designed to inhibit BMP signaling will block osteogenic-like calcification in diseased aortic valves. Likewise, inhibition of the Wnt/β-catenin signaling pathway may also serve to reduce aortic valve calcification during disease, which is supported by evidence from animal studies in ApoE knockout mice. When fed an atherogenic diet, ApoE knockout mice reportedly develop aortic valve calcification, however, when the Wnt co-receptor Lrp5 is genetically deleted in these mice, the amount of aortic valve calcification is significantly reduced . Therefore, Wnt inhibition may be another potential therapeutic approach for treating CAVD. Lastly, strategies to maintain Notch signaling in the valves may be another potential way to inhibit calcification in CAVD. Notch inhibition of calcification and osteogenic gene expression has been demonstrated in aortic VICs in culture and reduced Notch signaling in vivo leads to CAVD in mice [65, 153, 175]. Furthermore, Notch1 haploinsufficiency in humans is associated with CAVD, indicating that maintaining Notch signaling is important for valve homeostasis . Thus, therapeutic strategies designed to affect one or more of these pathways may serve to prevent valve calcification in CAVD. A potential limitation of this approach is that BMP, Wnt, and Notch signaling pathways are involved in many homeostatic and disease processes. For example, Wnt signaling is increased in many types of cancer, and all three pathways are involved in bone homeostasis. Therefore the development of therapeutics based on these molecular mechanisms must take into account potential effects on multiple organ systems. Nevertheless, targeted approaches based on these pathways could represent a new therapeutic avenue in the development of pharmacologic based approaches to CAVD.
6. Conclusions and future directions
There are numerous examples of shared molecular pathways between valvulogenesis, osteogenesis, and disease pathogenesis of CAVD. In valvulogenesis, signaling factors involved in early cushion formation, such as BMP, Notch, and Wnt/β-catenin pathways are active in osteogenesis and in CAVD [7-9, 14]. Furthermore, transcription factors expressed in the early valve mesenchyme, such as Twist1, Msx2, and Sox9, can also be found in the primitive condensed bone mesenchyme and in the mesenchymal-like cells identified in diseased aortic valve tissues [8, 13, 80, 255]. In addition to signaling and transcription factors, molecular pathways governing ECM production and remodeling, such as the RANKL – NFATc1 – CtsK pathway are shared amongst valve progenitor, developing bone, and diseased valve tissues [11, 47, 103, 106, 193]. This commonality suggests that the mesenchymal cells found within these tissues are governed by common molecular pathways and that these developmental pathways are reactivated during disease. Additional parallels can be drawn between calcification of the embryonic bone tissues and calcification observed in diseased aortic valves. For example, the endochondral bone factors Runx2, OCN, and BSP are reactivated during aortic valve disease, suggesting that osteogenic molecular pathways are activated during CAVD and may contribute to pathogenic calcification [5, 13, 76, 80, 106, 152]. Effective pharmacological therapies to treat CAVD remain elusive and identifying potential targets for new pharmacotherapies is a priority, as the only effective treatment for CAVD with AS is valve replacement surgery . Studies testing the effectiveness of statin therapy, inhibitors of the renin-angiotensin-aldosterone, and bisphosphonates in slowing the progression of CAVD have been disappointing (see therapeutic section). New therapeutic strategies are needed and, perhaps, targeted inhibition of BMP and Wnt signaling or maintenance of Notch signaling may provide new avenues for potential CAVD treatments.