About the book
During the last ten years, several technologies have evolved to allow the design and assembly of novel-unnatural biological systems, which is one of the main goals of synthetic biology. Currently, we are moving into a new wave of synthetic biology where building blocks for metabolic engineering can be designed and synthesized at an accessible price. This book aims to summarize the most recent advances on artificial gene synthesis such as enzymatic DNA synthesis, Chemical DNA synthesis, and High-Throughput Gene Synthesis, which are essential tools for the rational engineering of artificial metabolic pathways. Here, we will also discuss how the structural building blocks for metabolic engineering (Biobricks) can be obtained through a rational design process to ease pathway assembly. Thus, the state-of-the-art of several assembly methodologies such as Ligation-mediated assembly, PCR-mediated assembly, and Modular metabolic engineering will be discussed. Finally, a brief description of the most recent technologies to expand life's vocabulary through the construction of artificial nucleotides and their impact on research and industry will also be included in this book.