Relevant
\r\n\tVarious disciplines characterize the constituent components comprising mechatronics, which include physical systems modeling, sensors and actuators, signals and systems, computers and logic systems, and software and data acquisition.
\r\n\tMany new MEMS and Nanotechnology applications will emerge, expanding beyond that which is currently identified or known. Here are a few applications of current interest: new phenomena are addressed nowadays in science and technology.
\r\n\tIn this book, we will deal with physical systems modeling., discuss the physical phenomena involved, the adequate methodology to deal with them, and report a selection of papers recently published for possible applications for MEMS and Nanotechnology.
\r\n\r\n\tIn summary, Nano- and micro-electromechanical systems (NEMS/MEMS) are useful for applications ranging from: The design of MEMS accelerometers, gyroscopes, electrostatic actuators, and microresonators; Interfacial engineering for NEMS/MEMS; Biosensors, magnetic biochips, in vitro diagnostics, cell sorting, magnetic nanoparticles, spin electronic materials and sensors, magnetic inductive heads, and magnetic integrated inductors and transformer’s; Flexible substrates for electronics, sensors, and energy conversion platforms; Nanofabrication and nanopatterning technologies, including self-assembly for device fabrication.
\r\n\t
Who in biology has not heard of
The main reasons why
Typical
In terms of ecology,
Phylogenetically,
The building blocks of
This organism lacks many interesting features for biotechnology, such as growing at extreme temperatures or pH, the capacity to degrade toxic compounds, pollutants, or difficult to degrade polymers [23]. But as we will see later, this bacterium is capable of doing amazing things and its only limit is our imagination.
The advancement of genomic, transcriptomic, and proteomic technologies has led to the development of several online resources for the analysis of molecular and physiological aspects of
Name | URL | Description* |
---|---|---|
EcoCyc: Encyclopedia of | https://ecocyc.org | A comprehensive database joining together genomic information with biochemical features of |
PortEco | http://www.porteco.org | A resource for knowledge and data of the biology of |
EcoliWiki | http://ecoliwiki.net/ | Community-based pages about everything related to the biology of the nonpathogenic |
EcoGen 3.0 | http://ecogene.org/ | A database dedicated to analyzing and comparing genomic and transcriptomic data |
Kegg | http://www.genome.jp/kegg-bin/show_organism?org=eco | A powerful resource for understanding molecular datasets in different contexts, also easy access to gene information |
RegulonDB | http://regulondb.ccg.unam.mx | Database on transcriptional regulation in |
http://cgsc.biology.yale.edu/ | The CGSC Database of |
Relevant
*
Recently, using the Genome Conformation Capture technique, it was revealed that the linear organization of the genome is also true for the 3D structure, rendering neighboring genes to form small factories that are coregulated or coexpressed and showed a higher probability of forming protein-protein interactions. This organization represents two important aspects of bacterial genomes, first, the compactness in the 3D space, containing pathways in a nonrandom distribution, and second, genes that are closer to each other tend to be coexpressed and form protein-protein interactions favoring the concept of transcription factor even in microbial cells [27].
In the following sections, we will review what we consider the modern tools for genetic engineering
Without a doubt, plasmids are the most important tools not only for the manipulation of
Why plasmids are the basis for genetic engineering? By surveying the literature and commercial sources catalogs, there is a myriad of applications for plasmids: cloning, mutagenesis, protein fusion and overexpression, shuttle vectors from bacteria to a diverse range of hosts, among others. Plasmids must be first presented and then we provide some features that are relevant regarding the importance of plasmids in molecular biology and biotechnology.
Plasmids are extrachromosomal molecules that are self-replicative and sometimes provide interesting features to its host. The term was first coined by Joshua Lederberg in 1952 referring to genetic elements in bacteria that remained as an independent molecule from the chromosome at any stage of their replication cycle [28]. The definition was further refined to all the autonomously replicating DNA molecules to avoid including viruses. These molecules are present not only in eubacteria but also are found in Archea and some lower eukaryotic organisms [29]. In nature, many bacteria contain self-replicating DNA molecules that can be harnessed for molecular biology applications.
In the 1970s, the first generation of cloning plasmids was created, and from that moment on, research in the biological area was enriched with a powerful tool. Plasmids must contain several important features to be used in research: proper size for ease to transform or transfect, selection markers, a replication origin, regulatory elements to control expression, and transcription termination. All features are important when designing a plasmid vector for the desired application, the reader can imagine the goal, and there will always be a way to create the molecular tool for achieving such a goal, and that is possible due to the basic structure of most plasmids used in molecular biology and their modularity [31]. In Table 2, we summarize some of the most important features (modules) that plasmids must have in order to serve for different applications. We point out that sequence composition and structure, copy number, selection marker, and special features such as reporter proteins or regulatory elements are the most important features in a plasmid and can influence the outcome of the desired application.
Name | Type of element | Characteristics |
---|---|---|
ColE1 | Replication origin | Generates 15–20 copies of each plasmid molecule. Colicin production. Related to plasmids that confer immunity to phage infections [32]. Found in low copy plasmids such as pBR322 [33]. There are mutations in this replication origin that leads to high copy number plasmids, such as pUC series that can render up to 700 copies per cell [34, 35] |
p15A | Replication origin | Low copy number replication origin, estimated in 18–22 copies per cell [36]. This type of replication origin is often found in pACYC and its derivative vectors |
pMB1 | Replication origin | Versatile replication origin. The original sequence generates 15–20 copies per cell, but a mutant version can lead up to 700 copies per cell [37]. This plasmid contains the |
pSC101 | Replication origin | Five copies per cell [38] |
R6K | Replication origin | 15–20 copies per cell. Requires the π protein from the gene |
Ampr, Kanr, Cmr, Tetr among other | Selection markers | Elements required for the selection and maintenance of plasmids in bacterial hosts. Here are listed the resistance cassettes for Ampicillin, Kanamycin, Chloramphenicol, and Tetracycline, which are the most common selection markers. For additional markers, RAC database contains the information regarding antibiotic resistance traits and their sequence [40] or iGEM website for sequence modules bearing the proper syntax for synthetic constructs |
LacZ, CcdB, Green Fluorescent protein (GFP), etc | Additional elements required for positive clone selection, reporter protein fusions among others | Plasmids have been modified so that they contain multiple cloning sites with diverse unique restriction sites, counter selection for positive clone selection. Additional elements such as filamentous origins for single-stranded DNA generation for sequencing of high G+C templates or site-directed mutagenesis |
Common elements in plasmids used in molecular biology applications.
Additional sources: [31], http://blog.addgene.org/plasmid-101-origin-of-replication. https://www.neb.com/, https://www.thermofisher.com/mx/es/home/brands/invitrogen.html, and http://parts.igem.org/Help:Synthetic_Biology, which contains a repository of parts that can be needed for plasmid construction using synthetic methods.
Some exceptional features of plasmids are they can be used in systems where replication origins (check compatibility first) and selection markers can coexist in the same cell, which can be extremely useful for the coexpression of four different proteins in the same cell (e.g., the four plasmid system developed by Dykxhoorn et al., which are compatible between them [41]); the broad diversity of selection markers and partitioning control elements [30]; cloning capacity [31], which is an important feature for cloning large fragments required for synthetic biology applications or metabolic engineering; reporter proteins useful for selecting positive clones; recombination or assembly technology for easier cloning methods [42, 43], and the ability to be transferred from one host to another like the case of the OriV from RK2 plasmid [44]. We recommend for further information about replication control of plasmids to refer to del Solar et al. [45].
Linear plasmids are common in bacteria (particularly in actinobacteria), but thus far, only N15 plasmid prophage has been isolated from
One of the biggest impediments for plasmid segregation is the insert size. As discussed in Section 5, we are now facing not only the most exciting age of molecular biology but also the most challenging. In order to develop “bugs to the order,” i.e., microbes are “trained” to perform specific tasks [48], from simple pathways to synthesize a specific metabolite to complex genetic circuits that can be controlled for novel environmental responses. In all these cases, plasmids play an important role, from generating site-specific integration of chromosomal fusions to large plasmids that can hold complex constructs for further modification. Another challenging area is the generation of strains capable of producing metabolic products required for the pharmaceutical industry, where metabolic pathways and cell metabolism can be a strong impediment for proper synthesis and purification of relevant precursors [49].
As part of the information needed for plasmid manipulation, databases and repositories are also relevant for the manipulation and selection of the right plasmid for the applications you want to further exploit. Such examples are given: a powerful plasmid repository is Addgene (https://www.addgene.org/vector-database/) where you can get any plasmid in the repository with minimum fees for shipping and handling. This source is important in many aspects; you can gain knowledge about plasmid sequence, special features, creators, and availability to use since you can acquire them with small fees. Also, Harvard University is currently generating a plasmid repository but still under development (https://plasmid.med.harvard.edu/PLASMID/Home.xhtml). These repositories are an excellent option for accelerating research due to finding already generated constructs useful for ongoing projects. Also,
Novel methods such as Gibson assembly, Golden Gate assembly, and AQUA (advanced quick assembly) methods [43, 51, 52] have skyrocketed the possibility to assemble any plasmid with the desired characteristics. These methods are based on designed modules that can either be Polymerase Chain Reaction (PCR) amplified or generated as a complete synthetic construct and then assembled in the desired combination either by an enzymatic process (Gibson and Golden gate) or even enzyme-free methods such as AQUA.
Finally, plasmid biology is still under scrutiny, for their involvement in the mobility of traits that are important for human health such as antibiotic resistance, the distribution of pathogenicity islands, and genome evolution. Recently, novel tools for plasmid mining have been developed and uncover from Next Generation Sequencing data that plasmids can be uncovered and analyzed for further characterization [53, 54].
We are still in the process of truly knowing the potential of
One of the basic questions in biology is: what is life? Defining life imposes a challenging burden both intellectual and experimental. Many attempts have been done to answer this.
In
Larger genomic editions are needed to understand how far we can delete redundant or nonessential sequences. By using Cre/lox recombination, substantial genomic fragments can be deleted or sequentially removed, rendering the nonessential regions (regardless the genes present) from the genome [59–61].
Studies regarding genome size analyzed through deletions of specific genes or complete genomic regions have led on thinking about the minimal genome. In the case of
All these methods rely on basic bacterial genetics founded with
The most relevant study revealed that genome size has an impact on
After 6 years, in 2016, the first bacteria operating under a “minimal chemically synthesized genome” was created after the first fully functioning synthetic genome [64, 65].
This research we believe has an impact in the following areas. First, both studies settled the basis for whole genome synthetic biology, which will lead to important findings in many research areas. Second, the extensive transposon-based mutagenesis studies on the genome of
In biotechnology, biosensors are broadly defined as any device based on biological part, cell, tissue, or protein complex that are linked to a mechanical sensor or analytical receptor that provides a measurable signal proportional to the analyte in the reaction [66, 67].
In Figure 1, we depict the basic design for whole-cell biosensors and some applications. Plasmid vectors with all the possible modifications can lead to almost endless combinations. For practical applications, there are commercial vectors that can be used for such purposes or as mentioned in the previous sections, plasmid methods are powerful enough for fast and robust biosensor design.
Basic principles for biosensor design. First, the proper design and the experimental creation of reporter strains. With current knowledge, either plasmid creation or whole genome engineering can lead to the creation of a reporter strain. Second, incorporation of such elements into the host cell. Third, sensing module and response measurement. With current reporter proteins and detection technology, it is relatively easy to generate biosensors that can be used in different applications with high sensitivity and selectivity.
The basic design considers the following: copy number, reporter proteins, detection methods, and control elements. The latter is basically the most important feature. As shown in Table 1, the available databases provide enough information for promoter selection and design. Bioinformatic tools can make this process easier [71]. Also, generation and detection of this kind of biosensors are cost-effective and easy to generate and reasonably sensitive [71]. In terms of speed, sample analysis with whole-cell biosensors is fast and cheap in comparison with analytical methods. The sensitivity of analytical methods is higher and more accurate, but biosensors are a good alternative for fast detection of hazards. Also, they can be coupled with the controlled production of metabolites of commercial importance.
In the literature, there are several reports where
With the improvement of DNA synthesis, recoding protein-coding genes for the desired function is expanding the capabilities of transcription factors, and reporter proteins have created novel sensor modules. For example, XilR recoding has led to a sensor that can detect millimolar concentrations of trinitrotoluene and its derivative compounds [83]. By using shuttle vectors, we can generate biosensors that we can transfer from one host to another, which can provide information about differences in physiological responses during the exposure to a given environmental trait.
In the following section, we provide our final overview of the impact of
With the avenue of
Synthetic biology is a relatively new branch of molecular genetics that incorporate engineering principles for modifying several aspects of cell physiology, rewiring genetic circuits, creating novel circuits, and synthesizing custom-made DNA sequences and even genomes [87]. This particular branch of biology needs to be supported by an extensive knowledge of the organism that modifications or even whole genome synthesis is attempted, several novel tools for analyzing big datasets and molecular tools for that particular organism, for the generation of sequences and the computational design of DNA molecules, and a goal that can be achieved with the desired organism.
Multiplexing is the novel approach for redesigning organisms to do desired tasks [90]. By cycling through design-build–test framework we can achieve novel features in existing proteins and can further the advancement of genetic engineering. Thus far the most complicated and time-consuming part of this framework is the testing of the novel designs. High-throughput approaches have led to the development of fast and reliable screening methods and advances in this area, such as designed biosensors for the screening of metabolite producing strains or high-throughput methods for product screening, where the use of fluorescent proteins, colorimetric assays, and mass spectrometry are cornerstones for the development of screening methods for assessing success in strain engineering [90, 91]. DNA sequencing and synthesis coupled with good screening methods is the platform for future tools for the development of designed microorganism that can do desired tasks.
With all the technologies available, the advancement of using
Synthesis of important metabolites can be difficult, and researchers must face the stubbornness of microorganisms to redirect carbon flux to their own processes, rendering the production of relevant molecules costly and inefficient. But
Several aromatic compounds have been successfully synthesized in
Another relevant area was
Another important field where
Due to the modularity of polyketide synthases, they are excellent candidates for engineering, for either the production of novel compounds from existing gene clusters (through module shuffling, mutagenesis or deletions), or by the introduction of novel environmentally sequenced gene clusters and heterologous production [102, 103]
Therefore, the production of diverse metabolites or biological or industrially relevant molecules can be successfully achieved in
With all the research that is currently conducted in
With all the data available, we are on the verge of really important findings and novel biotechnological procedures using
In the rest of the biotechnology fields, we have enough information that suggests
The authors acknowledge the generous support from CONACyT-CIBIOGEM grant CB-4O-040116, CONACyT grant CB-2012-01 182671, and Guanajuato University grant 1081/2016 (DAIP). NVM acknowledge the support from CONACyT for a scholarship grant. We also acknowledge the support from Dr. Felipe Padilla Vaca.
Issues related to Industry 4.0 are constantly discussed among researchers, entrepreneurs, representatives of government agencies, and public organizations. Specifically, the impacts of the Industry 4.0 paradigm in the global and national economies, individual industries, employment, and capital markets are attracting more and more attention from economists. The global industrial environment has transformed dramatically in recent years as a result of technological advances and inventions. Industry 4.0 can be compared to three industrial revolutions that happened in the previous centuries and represent the most significant disruptive shifts in manufacturing as a result of technology advancements [1].
The advent of the steam engine accelerated the First Industrial Revolution, which began in Britain in the middle of the 18th century. The Second Industrial Revolution arose in Europe and the United States in the second mid-nineteenth century. This revolution had characterized by mass manufacturing and the substitution of chemical and electrical energy for steam. Many technologies and mechanization had been developed to meet the increased demand, allowing productivity to increase [2]. The Third Industrial Revolution was sparked by the creation of the Integrated Circuit (microchip). Using electronics and information technology to accomplish increased automation in manufacturing is a significant characteristic of this revolution, which arose in many industrialized countries around the world in the later years of the twentieth century [1].
Every industrial revolution centered around boosting productivity. The first three industrial revolutions had a significant impact on industrial operations, allowing for increased productivity and efficiency by utilizing innovative technological breakthroughs, such as steam engines, electricity, and digital technology [3]. Industry 4.0, which could ultimately be referred to as the fourth industrial revolution, is a highly complex framework that has been commonly debated and discovered. It has a significant impact on the industrial sector because it introduces relevant improvements related to smart and future factories. This developing Industry 4.0 concept is an umbrella term for a new industrial paradigm that includes Cyber-Physical Systems (CPS), the Internet of Things (IoT), the Internet of Services (IoS), Robotics, Big Data, Cloud Manufacturing, and Augmented Reality, etc. [4].
The adoption of these technologies, which will bring together the digital and physical worlds through embracing a set of future industrial developments, is essential in the development of further smart industrial processes. This adoption includes devices, machines, production modules, and products that can exchange information and control each other independently, resulting in a smart manufacturing environment [5]. This new approach will allow the improvement of productivity and efficiency, carrying enormous potential effects, and it will support a set of economic and social opportunities among the companies that are adopting this new manufacturing paradigm [1].
This chapter intends to provide clear insight into the current developments within Industry 4.0 phenomenon, due to the inconsistency within the existing literature, some stress positive effects of Industry 4.0, while others, negative ones. As a result, the purpose of our research is to provide a full explanation of the Industry 4.0 paradigm, as well as to determine whether or not it is appropriate for businesses, stockholders, and countries to adopt this new approach. This chapter gives a review of Industry 4.0 and definitions in the literature, as well as introduces a brief on Industry 4.0’s main components. Additionally, this chapter’s research methodology was based on papers related to Industry 4.0, which are the most recent and cited references. As well as this study differs from past studies in several aspects, as shown in 1) It conducts a comprehensive survey of all Fourth Industrial Revolution technologies or applications, whereas earlier literature focused on one or a few technologies. 2) It performs a case study of KUKA Corporation, a pioneer company in the manufacturing technologies and applications of the Fourth Industrial Revolution.
Thus, this chapter is structured in seven sections. After this introduction about the Industry 4.0 phenomenon. Section 2 answers the question “What is the industry 4.0?”, presenting two points: an overview or background about Industry 4.0, and provides a comprehensive definition of this concept, its visions. The key Industry 4.0 technology enablers or components of Industry 4.0 characteristics are described in Section 3, which is divided into ten parts. The characteristics of Industry 4.0 state in Section 4. Section 5 provides an analysis of the impacts and influence of this new industrial paradigm: industrial sector, business models and markets, work environment, work skills, economy and sustainability, the value chains, and supply chains. While Section 6 presents the key drivers and obstacles or barriers of the Industry 4.0 concept; also, this part presents a pioneering experience in implementing the applications of the Fourth Industrial Revolution technology “KUKA corporation.” Finally, Section 7 draws the main conclusions and findings of the Industry 4.0 vision and implications.
There have been three earlier industrial revolutions that have resulted in a transformation in manufacturing patterns: mechanization via water and steam power, mass production in assembly lines, and automating through computer and information technology [6].
The first industrial revolution
The
All systems are connected, resulting in “cyber-physical production systems” and, as a result, smart factories, in which production systems, components, and people interact through a network and production is almost autonomous. When these enablers are combined, Industry 4.0 has the potential to offer some amazing improvements in manufacturing environments. Machines that can foresee faults and initiate maintenance operations on their own, for example, or self-organized logistics that adapt to unexpected changes in production are examples (Figure 1) [9].
Represents a graphic illustration of the industrial revolutions overall. Source: Constructed by the author.
It also has the ability to alter people’s working habits. Individuals can be drawn into smarter networks by Industry 4.0, which might lead to more efficient working. The manufacturing environment’s digitization provides for more flexible means of providing the appropriate information to the right person at the right time. Maintenance personnel may now receive equipment documentation and service history more quickly and at the point of use, thanks to the growing usage of digital devices inside factories and out in the field. Maintenance personnel prefer to spend their time addressing issues rather than waste time looking for technical knowledge [10].
In a summary, Industry 4.0 is a game-changer in the industrial world. Manufacturing will alter as a result of digitization, including how things are manufactured and delivered, as well as how products are maintained and enhanced. As a result, it may legitimately claim to be the start of the fourth industrial revolution. Industry 4.0 is presently taking shape and its supporting technologies, such as the Internet of Things (IoT) and Cloud Manufacturing (CM), are, nevertheless, poorly defined, and under-researched.
Industry 4.0 is better known as the fourth industrial revolution and describes a future production system’s vision. The idea of Industry 4.0 was established by a group of professionals from several professions (such as business, politics, and academia) as part of an endeavor to integrate all manufacturing industries systems to achieve sustainability. The German government initially officially approved and implemented industry 4.0 for supporting automation in manufacturing, and for boosting German competitiveness in the manufacturing industry. Essentially, as a result of Industry 4.0, operations and manufactures will become further efficient and less expensive. These are accomplished through the simple interchange of information, integrated control of industrial goods and equipment, which work synchronously and intelligently in interoperability [11]. However, several researchers have different perceptions of the meaning of industry 4.0.
Kagermann, et al. [12] stress that industry 4 utilizes the power of communications technology and innovative inventions to boost the development of the manufacturing industry. Corresponding to Kagermann et al., the primary features of the industry 4.0 idea are characterized by three aspects: (1) horizontal integration, (2) vertical integration, and (3) end-to-end digital integration of engineering. Qin, Liu, and Grosvenor [13] emphasize that industry 4.0 encourages manufacturing efficiency by collecting data, making correct decisions. By using the most advanced technologies, the procedures will be easier. The interoperability operating ability to ensure a stable manufacturing environment. This overall consciousness gives Industry 4.0 the most important aspect of artificial intelligent functions.
The Fourth Industrial Revolution, 4IR, or Industry 4.0 conceptualizes rapid change to technology, industries, and societal patterns and processes in the 21st century due to increasing interconnectivity and smart automation [14]. Schwab pointed out that Industry 4.0 is one of the most important concepts in the development of global industry and the world economy, he accentuates that, Industry 4.0 is differentiated by a few characteristics of new technologies, the improvement in technologies is bringing significant effects on industries, economies, and governments’ development plans [15]. Industry 4.0 also denotes a social, political, and economic transformation from the digital age of the late 1990s and early 2000s to an era of embedded connection marked by widespread technological use (e.g., a metaverse). That, in comparison to humans’ inherent senses and industrial ability alone, we have constructed and are entering an augmented social reality [16].
Wang et al., [17] defined the fourth industrial revolution as the modern and more sophisticated machines and tools with advanced software and networked sensors that can be used to plan, predict, adjust, and control the societal outcome and business models. Thus, Industry 4.0 is an advantage to stay competitive in any industry. Also, Industry 4.0 can be perceived as a strategy for being competitive in the future. It is focused on the optimization of value chains due to autonomously controlled and dynamic production [18]. Furthermore, industry 4.0 is possible to indicate three future-relevant themes related to it, such as: dealing with complexity, capacity for innovation, and flexibility [19].
According to the concepts above, the majority of the researchers considered Cyber-Physical Systems (CPS), Internet of Things (IoT), Industrial Internet, and other topics to be part of Industry 4.0. Numerous authors also emphasized Industry 4.0 on the cost and profitability of recently created high-tech information and intelligent services. According to previous research on Industry 4.0, the early focus was mostly on the industrial manufacturing sector, but many industries are now adopting Industry 4.0, including automotive, engineering, chemical, and electronics. As a result, Industry 4.0 is aggregating existing ideas into a different value chain that leads to an improvement in transforming entire value chains of goods life cycles while developing innovative products in manufacturing, involving the connection of systems and things that create self-organizing and dynamic control within the organization.
Industry 4.0, often referred to as the fourth industrial revolution, is the vision or scenario of a future production process characterized by new levels of controlling, organizing, and transforming the entire value chain with the life cycle of products through three types of effective integration: horizontal, vertical, and end-to-end engineering integration, resulting in increased productivity and flexibility, the industry 4.0 leads to cost optimization and reduction [11]. The Cyber-Physical Systems (CPS), Internet of Things (IoT), artificial intelligence (AI), additive manufacturing, cloud computing, and other technologies are then combined to construct dynamic, real-time optimized, and self-organizing cross-company value networks. All of these components are necessary and integral to the futuristic Industry 4.0 concept.
Industry 4.0 is a complicated technical pattern characterized primarily by connection, integration, and industrial digitalization, highlighting the possibilities for integrating all components in a value-adding system. Digital manufacturing technology, network communication technology, computer technology, and automation technology are all included in this approach. Industry 4.0 technology breakthroughs are blurring the lines between the digital and physical worlds by merging human and machine agents, materials, products, production systems, and processes [20]. Industry 4.0 enables rapid technological advancements in a variety of areas; however, the emerging fourth industrial revolution is being shaped largely by the technical integration of Cyber-Physical Systems into manufacturing processes, as well as the use of the Internet of Things and Services in industrial processes [1]. As a result, this section gives a brief overview of each significant technology driver for Industry 4.0. It also is providing information on the basic components of Industry 4.0 or key technologies enablers for Industry 4.0, which consists of 10 components.
Cyber-Physical Systems (CPS) is the combination of computational and physical processes, which are essential components of Industry 4.0 implementations. They integrate imaging and control capabilities into the relevant systems. The ability of these systems to respond to any input generated is a key feature. They provide rapid control and verification of process feedback in order to generate predicted outputs. Bergera et al. (2016) defined cyber-physical sensor systems as part of cyberspace, special types of embedded systems, based on powerful software systems, enable integration in digital networks, and generate whole new system features [21]. Generally speaking, the evolution of a CPS is characterized by three phases. Identification technologies are included in first-generation CPS. Second-generation CPS is equipped with some sensors and actuators with a limited number of functions. In the third-generation CPS, data is kept and analyzed in addition to setting up the equipment. The CPS has many sensors and actuators and is meant to be network compatible. CPSs offer various features [19].
The CPS has several sensors and actuators and is meant to operate with a network. CPSs have features including quicker information access, preventative maintenance, pre-defined decision-making, and optimization processes. Also, CPS can boost consumers awareness and consciousness. Conversely, the CPS has certain security issues, which means that further usage will definitely result in increased dangers. It was pointed out that CPS equipment might cause disruptive societal changes since intelligent assistive or autonomous environments can cause mental illnesses, which can lead to bias toward new technology adoption and usage [21]. Cyber-Physical Systems have consisted of two key components: i) A virtual environment built through computer simulation of items and actions in the actual world, and ii) a network of objects and systems interacting with each other over the internet with a designated address [4].
The term “cloud” is utilized for applications, for instance, remote services, color management, and performance benchmarking applications. It has taken remarkable attention from the IT community, and its role in other business areas will continue to grow. Machines, data management, and functionality will continue to transition away from traditional ways and toward cloud-based solutions as technology improves. The cloud enables significantly faster distribution than standalone systems, as well as quick upgrades, current performance models, and other delivery possibilities [19].
The industry has found a significant shift toward cloud solutions, which will continue to develop and represent a substantial challenge to traditional data storage methods. Cloud technology is the most basic online storage service that gives operational comfort with web-based apps that do not require any installation. Cloud computing refers to the process of storing all applications, programs, and data on a virtual server. It improves efficiency by guaranteeing those input suppliers, employees, and consumers have access to the same information at the same time [22]. Cloud Systems lower costs, simplify infrastructure, expand work areas, safeguards data, and allow for instant access to information. There are four types of the system, mainly: i) Public Cloud; ii) Private Cloud; iii) Hybrid Cloud (combination of public and private cloud); 4) Community Cloud (this refers to the co-operation of any service on the cloud with a few companies) [9].
Cloud systems are an excellent source of Big Data (which might be organized or unstructured) management solutions. Because traditional computers may not be capable of managing large amounts of data, using a cloud system to do the necessary analysis, would be much easier and more efficient. As a result, data analysis and cloud systems should be inescapable components of Industry 4.0. The integration of cloud-connected robots into everyday life, as well as their impact, is considerable [4].
Machine to machine (M2M), refers to the technology that allows direct communication between devices using any channel, wired or wireless. Machine-to-machine communication can include industrial instrumentation and personal communications [23]. M2M is also considered to be an essential component of Industry 4.0. Machine to machine (M2M) is a technology that allows devices to communicate directly with one another over any channel, wired or wireless. Machine-to-Machine Communication can include industrial instrumentation and personal networks. M2M is also considered to be an essential component of Industry 4.0. The apps are geared toward adding value to the enterprises by introducing alternative revenue streams and reducing operational costs [24].
Ackermann (2013) clearly states that M2M operations have to enable aspects with different networked organizations including i) Remote Service and Asset Information Management delivering, which provide information federation and lifecycle support. ii) Connected Vehicles, which creates relationships and interactions. iii) Smart Vending, which includes retail, supply chain, and associated sub-elements [4]. The M2M vision has raised a number of issues, including establishing smart settings, smart architecture, and a smart grid with wireless sensors, as well as developing a communication language between machines and humans, as well as between humans in different locations [23].
The Internet of Things (IoT) is an emerging concept that combines various technologies and techniques, based on the interaction between physical things and the Internet. The advancement of technology in recent decades has enabled the Internet to be expanded into a new level known as “smart objects,” which is the foundation of an IoT vision, for this, the novel pattern consists in awarding ordinary things with intelligence, permitting them not only to accumulate information and cooperate with their surroundings, but also to be interrelated with other items, communicating information, and conducted a preliminary via the Internet. The growing interest in this field, which is widely regarded as one of the primary drivers of Industry 4.0, has produced the development of a number of visions and definitions for (IoT) [1].
The Internet of Things (IoT) refers to the interconnection of physical devices, cars, buildings, and other entities that are equipped with electronics, software, sensors, actuators, and network connections to gather and share data to create a smart manufacturing environment, also known as a smart factory [25]. Additionally, the concept of “The Internet of Services (IoS)” takes a similar approach to IoT but applies it to services rather than physical assets. The Internet of Services (IoS) idea will open up new prospects for the service sector by providing a commercial and technological foundation for the construction of business networks between service providers and clients [4].
The expansion of IoT in industrial contexts and value chains will give several opportunities for users, manufacturers, and businesses, having a significant influence in a variety of industries. The Internet of Things is breaking new ground, with a slew of new applications emerging around three key pillars: i) process optimization; ii) resource optimization, and iii) the building of sophisticated autonomous systems. IoT technology will continue to evolve and spread, allowing objects to become smarter, more dependable, and autonomous, allowing for the supply of higher-value products and services [1]. On the other hand, the effectiveness of Industry 4.0 depends upon existing network infrastructure, the intelligence, and human knowledge embedded into the system [22].
“Dark factories,” “lights off factories,” and “unmanned factories” are all terms used to describe smart factories, this system is integrated with the small intervention of human beings. The individual is entering into these systems mainly in the problem-solving stages. The concept known as Lights out (dark) or unmanned factories nowadays is an automation and autonomy enhanced methodologies including equipment used in factories that actively operate the production [4, 26]. The most famous characteristic of dark factories is that they do need no human power. In unmanned factories, there is not enough time to enter the plant from the raw material to the exit from the factory. That is to say that in these factories, production is carried out entirely with robotic systems [18]. It is self-evident that smart factories will have the characteristics and procedures required by the Fourth Industrial Revolution. And these processes, which are of great importance to our future of production. Furthermore, the essential activity for generating a smart factory running under Industry 4.0 is integrating different other components together, such as big data, CPS, cloud, IoT, M2M, etc. [4]
There are many challenges that determine the formation of smart factories, such as the availability of energy and its supply, the efficiency of the labor, and the availability of the technological infrastructure necessary to shift toward smart factories. On the other hand, these factories will have a negative impact on existing employment and increase unemployment rates [7].
Every day, new goods and systems emerge as a result of technological advancements. Flying automobiles, holographic television, and hundreds of electrical devices to be implanted into the human body are all possibilities [26]. Humanoid robots will be a part of everyday life in the not-too-distant future. Recent innovations have brought about skills that empower robots to control their environment. Artificial intelligence will contribute to the development of having robot teams cooperating and collaborating in achieving certain tasks defined for a specific purpose [28].
Implementing a collaborative robot in a factory will provide several benefits for the company, including i) preventing humans from performing repetitive, non-ergonomic, and dangerous work; ii) producing high-quality products with favorable cost–benefit ratios while also increasing productivity; and iii) increasing competitiveness in comparison to countries with cheap labor [29]. When a robot is used in a productive process, the benefits of the robot utilization are combined with the effort of an operator. There is no teamwork between the man and the robot on the first level. The workplace is totally shared between the man and the robot at the final level [30].
Simulation and augmented reality (AR) is a type of enhanced reality in which live direct or indirect views of physical real-world environments are augmented with computer-generated visuals projected on top of them. Industry 4.0 applications rely heavily on this technology. This innovative technology, which is critical to the industrial revolution, was created by combining real operations and simulation industries [4]. These strategies have a lot of advantages, especially when it comes to creating products and manufacturing processes. One of the cutting-edge technologies included in the Industry 4.0 trend is augmented reality, which is particularly useful in producing smart manufacturing functions [28].
Enterprise resource planning (ERP) refers to information systems that are designed to integrate and efficiently employ all of an organization’s resources. An ERP software is a system that supports an organization in bringing together processes and data that are executed all over the processes (suppliers, production, stock, sales). ERP systems are able to provide an integrated approach to information use, to start forecasting and extracting information, which can use in various departments [4]. There is a connection between big data and Industry 4.0, Manufacturing Executive Systems (MES), cloud systems, and ERP are integrated. It is critical that all procedures in the design stage as well as the customer journey are compatible with the Industry 4.0 approach. The ERP process is also a vital component in this framework [28].
The idea of Industry 4.0 necessitates connection and collaboration criteria. End-user feedback is critical, as is providing immediate additional value to all interested parties. In order for personalization to be possible, network systems must be intelligent [22]. A telecom operator may be able to analyze network performance during fluctuations and use preventive scenarios to reduce client dissatisfaction. A well-structured ERP system can enable these characteristic features. ERP systems can help with Industry 4.0 implementations, especially as a result of the following advantages: i) Real-time data may be evaluated and allow for early detection; ii) ERP systems can provide sales and purchasing transparency; iii) ERP data may be used by mobile applications to communicate; iv) Optimum resource utilization may be achieved under varying job descriptions; v) Clients may be able to track their orders online and receive the necessary information quickly [4].
The Smart Virtual Product Development (SVPD) system is a product development decision support technology that saves, uses, and shares the experiential knowledge of previous decisional events in the form of SOEs. It was created to address the requirement for digital knowledge captured in smart manufacturing product design, production planning, and inspection planning. As a result, product quality and development time will be improved, as required by Industry 4.0 concepts [31].
The core progress from traditional manufacturing toward Industry 4.0 concluded into four key features and characteristics [32]: (1) vertical networking of smart manufacture schemes; (2) horizontal integration through a new generation of global value chain networks; (3) through-life engineering across the entire value chain; and (4) the impact of exponential technologies.
Industry 4.0′s first main characteristic is the vertical networking of smart manufacturing systems. Vertical integration in Industry 4.0 establishes a connection between the many levels of the industry, from the manufacturing floor up, via production monitoring, control, and supervision, quality management, operations, product management, processing, and so on. This interconnectedness across all corporate levels provides for a fluid, transparent data flow, allowing for data-driven strategic and tactical choices [20]. Hence, the main objective behind vertical networking is to utilize Cyber-Physical Production Systems (CPPSs), to enable industries to quickly respond to unexpected order changes resulting from demand fluctuations, equipment failure or stock shortage. Vertical networking improves an organization’s capacity to adequately adapt to changes in market requirements and benefit from new possibilities [22].
Furthermore, it makes it easier to link resources to goods and find supplies and parts at any time. Similarly, processing data, anomalies, and defects from various processing stages of the manufacturing line are automatically captured and registered, allowing for quick responses to order changes, quality variations, and even machinery breakdowns. As a consequence, waste is decreased, and resource efficiency, notably in terms of material usage, energy consumption, and human resources is improved [28].
In the Industry 4.0 concept, horizontal integration refers to the network of diverse processes, companies, and services that make up a product’s global value chain. This can be viewed at the production level as a total consolidation of all associated manufacturing processes. Vertical integration, on the other hand, refers to a high level of coordination between production and top management layers such as quality management, product management, and production control [33].
The horizontal integration in an Industry 4.0 enterprise occurs at different levels: production floor, multiple production facilities, and entire value chain. Each connected machine or production unit becomes a node with well-defined properties within the production network. These nodes continuously communicate their status to respond autonomously to dynamic production requirements cost-effectively and reduce system downtime through predictive maintenance . If an enterprise owns several production sites, the horizontal integration enables to share inventory levels and unexpected delays, and possibly redistribute work among owned facilities to respond to market demand fluctuations rapidly or increase the efficiency and speed of the production process. However, the most critical and global horizontal integration remains the integration across the entire value chain [12].
Industry 4.0 offers a highly automated and transparent collaboration across the complete value chain, using CPPSs, from the inbound assembly, packaging, storing, production, quality control, marketing, and sales, to outbound distribution, logistics, and retail services. The horizontal integration across all these activities creates a transparent value chain that is updated in real-time. Hence, this feature provides a high level of flexibility to respond more rapidly to changing market demands, shortcomings, and problems, facilitates the optimization of the production process, increases its efficiency, and reduces the generated waste [17]. Additionally, the fact that any part or product’s history is logged and can be accessed at any time ensures constant traceability, also known as “product memory” [19].
Among the characteristics of the Fourth Industrial Revolution is also the impact of the ten components of the 4th Industrial Revolution
Innovation and scientific advancements perform an essential role in businesses, sectors, and countries. However, the digital improvements and the increasing interconnectivity will bring additional challenges and upgrades to societies, since, Industry 4.0 (Ir 4.0) will significantly change the manufacturing systems in terms of design, processes, operations, and services. Industry 4.0 will lead to potential deep changes in a variety of fields outside of the industrial sector. Its influence and effect may be divided into six categories: (1) Industry sector, (2) Products and services, (3) Business models, entrepreneurship, and market competition, (4) Economies of nations, (5) Work environment, and (6) Skills development.
The industry sector will be the first to feel the effects of Industry 4.0. This new industrial paradigm will usher in a vision of manufacturing that is decentralized and digitalized, with production elements that can autonomously govern themselves, trigger operations, and adapt to changes in their surroundings. Furthermore, the developing paradigm recommends fully integrating products and processes, altering industrial vision from mass production to mass customization, resulting in increased complexity [35]. Consequently, advanced technologies and the building of smart factories will have a significant impact on production processes and operations, providing for greater operational flexibility, and more efficient utilization of resources. Industry 4.0 will have a considerable effect on the production systems, supply chains, and industrial activities. This new paradigm is changing the current industrial landscape in three ways: (1) production digitization, (2) automation, and (3) integrating the manufacturing site to a larger supply chain. Industry 4.0, in this sense, entails complete network integration and real-time data sharing [1]. Productivity growth is at the core of each industrial revolution. The 4th industrial revolution, on the other hand, will influence the entire supply chain, from product creation and manufacturing to outbound logistics, in addition to enhancing productivity [36].
ROJKO, et al. (2020) used the vector autoregression model forecast for data from the manufacturing sector in the United States over the period (2008−2018) and concluded that, the share of manufacturing output and employment has declined, and that the manufacturing sector has reached a turning point, after which robotization can increase employment and labor productivity of workers while also stimulating further growth of their education levels. They concluded that the shift to Industry 4.0 has a significant impact on the growing demand for new knowledge and skills in order to boost productivity. As a result, anticipated growths of assessed manufacturing indicators imply that the negative effects of robotization in the recent past were only transient, as the Industry 4.0 age has begun. Nonetheless, further policies are needed to enable long-term industry development [37].
This new industrial paradigm has a significant impact on products and services. Rapid changes in the economic landscape and dynamic market demands have resulted in an increased demand for the development of more complicated and intelligent products in recent years [36]. Products will become increasingly modular and configurable, allowing for mass customization to match individual consumer needs [35]. As a result, Industry 4.0 is defined by the emergence of new products and services as embedded systems that can become attentive and interactive, be managed, and tracked in real-time, optimize the entire value chain, and provide pertinent information about their status throughout their lifecycle [37].
In the previous few years, company models and markets have swiftly altered, and new inventive business models will emerge. In the context of Industry 4.0, the introduction of new disruptive technologies has altered the way products and services are sold and delivered, disrupting established enterprises, and introducing new business prospects and models [33]. As a result, value chains are becoming more responsive, as Industry 4.0 encourages integration between manufacturers and customers, allowing for closer customer connection and business model adaption to market demands. The rising digitalization of industrial production, combined with system integration and complexity, will result in the establishment of increasingly sophisticated and digital market models, boosting competitiveness by removing barriers between information and physical structures [1].
Because of technological advancements, the workplace environment is changing fast, and Industrial revolution 4.0 is redefining jobs and key competencies. The most significant transition is the human-machine connection, which includes employee contact and a set of new collaborative work approaches [18]. The number of robots and intelligent technologies is growing, the real and virtual environments are merging, implying the existing work environment is undergoing a considerable transition [13].
The rising importance of human-machine interfaces will encourage interaction between production elements as well as the necessary communication between smart machines, smart products, and employees, which will be aided by CPS’ vision of IoT and IoS. As a result, ergonomic concerns should be considered in the context of Industry 4.0, and future systems should emphasize the relevance of workers. Job profiles, as well as work management, organization, and planning will be affected by the integration of Industry 4.0 in industrial systems and the rising deployment of new technologies [12]. In this scenario, the major task is to avoid technological unemployment by reframing present jobs and taking steps to adapt the workforce to the new jobs that will be generated [28].
One of the most significant fundamental factors for a successful acceptance and implementation of the Industry 4.0 framework is skill development, which will lead to demographic and societal changes. New competencies will be required in the future work vision, and it will be vital to provide opportunities for the acquisition of these abilities through high-quality training. This new industrial paradigm will have a significant impact on the labor market and professional roles, and it will be critical to ensure that more jobs are generated than are lost [26].
Interdisciplinary thinking will be vital, and outstanding abilities in social and technological domains will be desired. The new required competency sectors must be included in schooling. As a result of Industry 4.0’s rising automation of jobs, workers must be prepared to take on new responsibilities [28]. The same can be said for engineering education, which has a lot of promise in terms of training future professionals and informing them about new technical trends and opportunities, as well as managers who need to adapt their management strategies to meet changing market demands. Furthermore, in order to address Industry 4.0, more qualified personnel will be required in technological sectors [1].
In summary, Industry 4.0 has enormous potential in many areas, and its implementation will have an impact across the entire value chain, improving production and engineering processes, improving product and service quality, optimizing customer-organization relationships, bringing new business opportunities and economic benefits, changing educational requirements, and transforming the current work environment.
An economy can be inspired by the introduction of new models and emerging technological improvements. Digitization involves the convergence between physical and virtual worlds and will have a widespread impact in every economic sector [15]. This will be the primary driving force behind innovation, which will be crucial to productivity and costs of production, which is reflected in the competitiveness (companies, sectors, and nations) [17].
Industry 4.0 also, can transform existing relationships in the manufacturing process, allowing the manufacturing sector to join the information age by allowing communication at all stages of the manufacturing process. Some academics anticipate that Industry 4.0 would lead to new economic forms in the industry, agriculture, and services [3]. The majority of businesses expect a two-year payback on their Industry 4.0 investments, which leads to a considerable rise in investment in this area is likely, it’s reflected in economic growth [37].
On the other hand, some experts believe that Industry 4.0 will result in increased inequality due to its threat of disrupting labor markets. It is argued that the continuous growth in automation, robots, and computers will take the jobs of workers in many industries with the most worrying factor being the increased danger of the disappearance of low-skill/low-pay jobs which will cause a lot of challenges for the poor, which will lead to a rise in social tensions [37]. The most concerning fact in Industry 4.0 is that it is not only the transfer of labor from one sector of the economy to another but also the availability of technology that will replace human capital, in other words, taking people’s jobs. The technological revolution will also have an impact on topics such as material or ideological changes brought about by the introduction of new gadgets or systems, all of which will have an impact on redefining humanity’s culture [3].
In general, digitization and interconnection of industrial processes, lead to potentials in all three dimensions of sustainability. However, achieving long-term benefits of sustainability is accompanied by several challenges respectively, especially in the implementation phase of Industry 4.0 [38].
Regarding the social dimension of Industry 4.0, several benefits for employees are named, such as improved human learning through intelligent assistance systems as well as human-machine interfaces that lead to increased employee satisfaction in industrial workplaces [8, 22]. However, current literature cannot provide a unified perspective on whether Industry 4.0 will cause an increase or decrease in employee numbers in the industry. In this regard, concrete numbers named differ to a large extent [3, 15]. In general, a further replacement of simple tasks is expected, whereas tasks such as monitoring, collaboration, and training will still be required [3]. Hereby, new job profiles with novel requirements for training and education are expected to emerge, mostly referring to decreasing importance of manual labor in contrast to IT skills. On the other hand, tasks that include planning and monitoring, as well as decision-making, could fall to autonomous systems, therefore, possibly replacing jobs in this area.
The fourth industrial revolution has a significant impact on supply chain interactions, which is mainly due to the exponential growth of sensible data and the widespread of digitalized processes [40]. To understand the impact of the adoption and exploitation of Industry 4.0 technologies on the value chains and supply chains (SC). Based on the review, the effect of Industry 4.0 implementation on the supply chains (SC) are identified as follows:
Despite the rapid rise of Industry 4.0, research related to the identification of potential drivers and hurdles to its implementation are scarce. To better understand the motivations and challenges to the adoption and use of Industry 4.0 technologies, a literature review was conducted. The following are the primary drivers for Industry 4.0 implementation, as determined by the review:
In this section, we introduce an overview of some applications of the Fourth Industrial Revolution. Also, we provide a case study for these applications by
The “
Similarly, using techniques such as
Another application of industry 4.0 in the
There are several applications for industry 4.0, for example, the KUKA corporation which works in the areas, for instance, smart factories, M-2-M, computing cloud, intelligent robots, e-commerce, and so on.
There are also some intimidating resisting forces, barriers, for implementing Industry 4.0 practices. These obstacles may be classified under the following business dimensions:
Additionally,
This study contributes to bridging the critical gap, by discussing the key components, characteristics, effects on many dimensions, drivers, barriers, and other implementation challenges of Industry 4.0, the fourth industrial revolution describes a future production system’s vision. Industry 4.0 is an inevitable revolution covering a wide range of innovative technologies, such as cyber-physical systems, RFID technologies, IoT, cloud computing, big data analytics, advanced robotics, smart factories, etc. The Industry 4.0 paradigm is transforming business in many industries, e.g., automotive, logistics, aerospace, and energy sectors, etc. Industry 4.0 realizes the development and integration of information and communication technologies into business processes. The capabilities or components of Industry 4.0 bring significant advantages to organizations, including customization of products, real-time data analysis, increased visibility, autonomous monitoring and control, dynamic product design and development, enhanced productivity, and competitiveness.
The key characteristic features of Industry 4.0 are collaboration and integration of schemes, both horizontal and vertical. In vertical integration, Information and Communication Technology (ICT) is integrated into various hierarchical levels of the organization, from floor-level control to production, operations, and management levels. This vertical integration networking empowers the use of components of Industry 4.0 for production to respond to demand disparity or the fluctuations in stock levels. In horizontal integration, ICT is used to exchange information between many players. Integration of these systems for a flawless collaboration, integration, and exchange of data with all the stakeholders is a complicated scenario. Implementation of Industry 4.0 apps support to reduce costs, improves productivity, efficiency, and flexibility, and enhance product customization.
Innovation and technological advancements perform an essential role in organizations, sectors, countries. However, the digital transformation improvements and the rising interconnectivity will bring new challenges to societies, since Industry 4.0 will significantly change the products and manufacturing systems regarding design, processes, operations, and services. Industry 4.0 uses several advanced tools and technologies, thus helping to redefine conventional industrial processes. Industry 4.0 has enormous potential effect in many areas, and its application will have an impact across the entire value chain, improving production and engineering processes, improving product and service quality, optimizing customer-organization relationships, bringing new business opportunities and economic benefits, changing educational requirements, and transforming the current work environment. Digitization and interconnection of industrial processes (Industry 4.0), leading to potentials in all three dimensions of sustainability.
There are several applications for industry 4.0, applied by the KUKA corporation which works in the areas, for instance, smart factories, M-2-M, computing cloud, intelligent robots, e-commerce, etc., these technologies or applications help the industry 4.0 to separate rapidly. On the other hand, there are also some barriers, for implementing Industry 4.0 practices. These obstacles may be classified into many business dimensions: financial constraints, technical competency of the focal, organizational nature, lack of management support and resistance to change, legal issues, lack of policies and support from the government.
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Other positions she has held at the university include Vice-Dean of Master Programs, Vice-Dean of the Degree in Biology and Vice-Dean for Mobility and Enterprise and Engagement at the Faculty of Science (University of Alicante). She received her Bachelor in Biology in 1998 (University of Alicante) and her PhD in 2003 (Biochemistry, University of Alicante). She undertook post-doctoral research at the University of East Anglia (Norwich, U.K. 2004-2005; 2007-2008).\nHer multidisciplinary research focuses on investigating archaea and their potential applications in biotechnology. She has an H-index of 21. She has authored one patent and has published more than 70 indexed papers and around 60 book chapters.\nShe has contributed to more than 150 national and international meetings during the last 15 years. Her research interests include archaea metabolism, enzymes purification and characterization, gene regulation, carotenoids and bioplastics production, antioxidant\ncompounds, waste water treatments, and brines bioremediation.\nRosa María’s other roles include editorial board member for several journals related\nto biochemistry, reviewer for more than 60 journals (biochemistry, molecular biology, biotechnology, chemistry and microbiology) and president of several organizing committees in international meetings related to the N-cycle or respiratory processes.",institutionString:null,institution:{name:"University of Alicante",institutionURL:null,country:{name:"Spain"}}},editorTwo:null,editorThree:null},{id:"15",title:"Chemical Biology",coverUrl:"https://cdn.intechopen.com/series_topics/covers/15.jpg",isOpenForSubmission:!0,editor:{id:"441442",title:"Dr.",name:"Şükrü",middleName:null,surname:"Beydemir",slug:"sukru-beydemir",fullName:"Şükrü Beydemir",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y00003GsUoIQAV/Profile_Picture_1634557147521",biography:"Dr. Şükrü Beydemir obtained a BSc in Chemistry in 1995 from Yüzüncü Yıl University, MSc in Biochemistry in 1998, and PhD in Biochemistry in 2002 from Atatürk University, Turkey. He performed post-doctoral studies at Max-Planck Institute, Germany, and University of Florence, Italy in addition to making several scientific visits abroad. He currently works as a Full Professor of Biochemistry in the Faculty of Pharmacy, Anadolu University, Turkey. Dr. Beydemir has published over a hundred scientific papers spanning protein biochemistry, enzymology and medicinal chemistry, reviews, book chapters and presented several conferences to scientists worldwide. He has received numerous publication awards from various international scientific councils. He serves in the Editorial Board of several international journals. 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He is a member of the Turkish Biochemical Society, American Chemical Society, and German Genetics society. Dr. Ekinci published around ninety scientific papers, reviews and book chapters, and presented several conferences to scientists. He has received numerous publication awards from several scientific councils. 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Since then, he has been working as an Adjunct Professor in the same Department at the University of Pavia. His research activity during the first years was primarily focused on the purification and structural characterization of enzymes from animal and plant sources. During this period, Prof. Iadarola familiarized himself with the conventional techniques used in column chromatography, spectrophotometry, manual Edman degradation, and electrophoresis). Since 1995, he has been working on: i) the determination in biological fluids (serum, urine, bronchoalveolar lavage, sputum) of proteolytic activities involved in the degradation processes of connective tissue matrix, and ii) on the identification of biological markers of lung diseases. 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She gained considerable experience in developing and validating new methodologies whose applications allowed her to determine both the amount of biomarkers (Desmosine and Isodesmosine) in the urine of patients affected by COPD, and the activity of proteolytic enzymes (HNE, Cathepsin G, Pseudomonas aeruginosa elastase) in the sputa of these patients. Simona Viglio was also involved in research dealing with the supplementation of amino acids in patients with brain injury and chronic heart failure. She is presently engaged in the development of 2-DE and LC-MS techniques for the study of proteomics in biological fluids. The aim of this research is the identification of potential biomarkers of lung diseases. 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She is now a lecturer at the University of Witwatersrand, South Africa, and a principal researcher at the Health Economics and Epidemiology Research Office (HE2RO), South Africa. Dr. Moolla holds a Ph.D. in Psychology with her research being focused on mental health and resilience. In her professional work capacity, her research has further expanded into the fields of early childhood development, mental health, the HIV and TB care cascades, as well as COVID. She is also a UNESCO-trained International Bioethics Facilitator.",institutionString:"University of the Witwatersrand",institution:{name:"University of the Witwatersrand",country:{name:"South Africa"}}},{id:"419588",title:"Ph.D.",name:"Sergio",middleName:"Alexandre",surname:"Gehrke",slug:"sergio-gehrke",fullName:"Sergio Gehrke",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y000038WgMKQA0/Profile_Picture_2022-06-02T11:44:20.jpg",biography:"Dr. Sergio Alexandre Gehrke is a doctorate holder in two fields. The first is a Ph.D. in Cellular and Molecular Biology from the Pontificia Catholic University, Porto Alegre, Brazil, in 2010 and the other is an International Ph.D. in Bioengineering from the Universidad Miguel Hernandez, Elche/Alicante, Spain, obtained in 2020. In 2018, he completed a postdoctoral fellowship in Materials Engineering in the NUCLEMAT of the Pontificia Catholic University, Porto Alegre, Brazil. He is currently the Director of the Postgraduate Program in Implantology of the Bioface/UCAM/PgO (Montevideo, Uruguay), Director of the Cathedra of Biotechnology of the Catholic University of Murcia (Murcia, Spain), an Extraordinary Full Professor of the Catholic University of Murcia (Murcia, Spain) as well as the Director of the private center of research Biotecnos – Technology and Science (Montevideo, Uruguay). Applied biomaterials, cellular and molecular biology, and dental implants are among his research interests. He has published several original papers in renowned journals. In addition, he is also a Collaborating Professor in several Postgraduate programs at different universities all over the world.",institutionString:null,institution:{name:"Universidad Católica San Antonio de Murcia",country:{name:"Spain"}}},{id:"342152",title:"Dr.",name:"Santo",middleName:null,surname:"Grace Umesh",slug:"santo-grace-umesh",fullName:"Santo Grace Umesh",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/342152/images/16311_n.jpg",biography:null,institutionString:null,institution:{name:"SRM Dental College",country:{name:"India"}}},{id:"333647",title:"Dr.",name:"Shreya",middleName:null,surname:"Kishore",slug:"shreya-kishore",fullName:"Shreya Kishore",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/333647/images/14701_n.jpg",biography:"Dr. Shreya Kishore completed her Bachelor in Dental Surgery in Chettinad Dental College and Research Institute, Chennai, and her Master of Dental Surgery (Orthodontics) in Saveetha Dental College, Chennai. She is also Invisalign certified. She’s working as a Senior Lecturer in the Department of Orthodontics, SRM Dental College since November 2019. She is actively involved in teaching orthodontics to the undergraduates and the postgraduates. Her clinical research topics include new orthodontic brackets, fixed appliances and TADs. She’s published 4 articles in well renowned indexed journals and has a published patency of her own. Her private practice is currently limited to orthodontics and works as a consultant in various clinics.",institutionString:null,institution:{name:"SRM Dental College",country:{name:"India"}}},{id:"323731",title:"Prof.",name:"Deepak M.",middleName:"Macchindra",surname:"Vikhe",slug:"deepak-m.-vikhe",fullName:"Deepak M. Vikhe",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/323731/images/13613_n.jpg",biography:"Dr Deepak M.Vikhe .\n\n\t\n\tDr Deepak M.Vikhe , completed his Masters & PhD in Prosthodontics from Rural Dental College, Loni securing third rank in the Pravara Institute of Medical Sciences Deemed University. He was awarded Dr.G.C.DAS Memorial Award for Research on Implants at 39th IPS conference Dubai (U A E).He has two patents under his name. He has received Dr.Saraswati medal award for best research for implant study in 2017.He has received Fully funded scholarship to Spain ,university of Santiago de Compostela. He has completed fellowship in Implantlogy from Noble Biocare. \nHe has attended various conferences and CDE programmes and has national publications to his credit. His field of interest is in Implant supported prosthesis. Presently he is working as a associate professor in the Dept of Prosthodontics, Rural Dental College, Loni and maintains a successful private practice specialising in Implantology at Rahata.\n\nEmail: drdeepak_mvikhe@yahoo.com..................",institutionString:null,institution:{name:"Pravara Institute of Medical Sciences",country:{name:"India"}}},{id:"204110",title:"Dr.",name:"Ahmed A.",middleName:null,surname:"Madfa",slug:"ahmed-a.-madfa",fullName:"Ahmed A. Madfa",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/204110/images/system/204110.jpg",biography:"Dr. Madfa is currently Associate Professor of Endodontics at Thamar University and a visiting lecturer at Sana'a University and University of Sciences and Technology. He has more than 6 years of experience in teaching. His research interests include root canal morphology, functionally graded concept, dental biomaterials, epidemiology and dental education, biomimetic restoration, finite element analysis and endodontic regeneration. Dr. Madfa has numerous international publications, full articles, two patents, a book and a book chapter. Furthermore, he won 14 international scientific awards. Furthermore, he is involved in many academic activities ranging from editorial board member, reviewer for many international journals and postgraduate students' supervisor. Besides, I deliver many courses and training workshops at various scientific events. Dr. Madfa also regularly attends international conferences and holds administrative positions (Deputy Dean of the Faculty for Students’ & Academic Affairs and Deputy Head of Research Unit).",institutionString:"Thamar University",institution:null},{id:"210472",title:"Dr.",name:"Nermin",middleName:"Mohammed Ahmed",surname:"Yussif",slug:"nermin-yussif",fullName:"Nermin Yussif",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/210472/images/system/210472.jpg",biography:"Dr. Nermin Mohammed Ahmed Yussif is working at the Faculty of dentistry, University for October university for modern sciences and arts (MSA). Her areas of expertise include: periodontology, dental laserology, oral implantology, periodontal plastic surgeries, oral mesotherapy, nutrition, dental pharmacology. She is an editor and reviewer in numerous international journals.",institutionString:"MSA University",institution:null},{id:"204606",title:"Dr.",name:"Serdar",middleName:null,surname:"Gözler",slug:"serdar-gozler",fullName:"Serdar Gözler",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/204606/images/system/204606.jpeg",biography:"Dr. Serdar Gözler has completed his undergraduate studies at the Marmara University Faculty of Dentistry in 1978, followed by an assistantship in the Prosthesis Department of Dicle University Faculty of Dentistry. Starting his PhD work on non-resilient overdentures with Assoc. Prof. Hüsnü Yavuzyılmaz, he continued his studies with Prof. Dr. Gürbüz Öztürk of Istanbul University Faculty of Dentistry Department of Prosthodontics, this time on Gnatology. He attended training programs on occlusion, neurology, neurophysiology, EMG, radiology and biostatistics. In 1982, he presented his PhD thesis \\Gerber and Lauritzen Occlusion Analysis Techniques: Diagnosis Values,\\ at Istanbul University School of Dentistry, Department of Prosthodontics. As he was also working with Prof. Senih Çalıkkocaoğlu on The Physiology of Chewing at the same time, Gözler has written a chapter in Çalıkkocaoğlu\\'s book \\Complete Prostheses\\ entitled \\The Place of Neuromuscular Mechanism in Prosthetic Dentistry.\\ The book was published five times since by the Istanbul University Publications. Having presented in various conferences about occlusion analysis until 1998, Dr. Gözler has also decided to use the T-Scan II occlusion analysis method. Having been personally trained by Dr. Robert Kerstein on this method, Dr. Gözler has been lecturing on the T-Scan Occlusion Analysis Method in conferences both in Turkey and abroad. Dr. Gözler has various articles and presentations on Digital Occlusion Analysis methods. He is now Head of the TMD Clinic at Prosthodontic Department of Faculty of Dentistry , Istanbul Aydın University , Turkey.",institutionString:"Istanbul Aydin University",institution:{name:"Istanbul Aydın University",country:{name:"Turkey"}}},{id:"240870",title:"Ph.D.",name:"Alaa Eddin Omar",middleName:null,surname:"Al Ostwani",slug:"alaa-eddin-omar-al-ostwani",fullName:"Alaa Eddin Omar Al Ostwani",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/240870/images/system/240870.jpeg",biography:"Dr. Al Ostwani Alaa Eddin Omar received his Master in dentistry from Damascus University in 2010, and his Ph.D. in Pediatric Dentistry from Damascus University in 2014. Dr. Al Ostwani is an assistant professor and faculty member at IUST University since 2014. \nDuring his academic experience, he has received several awards including the scientific research award from the Union of Arab Universities, the Syrian gold medal and the international gold medal for invention and creativity. Dr. Al Ostwani is a Member of the International Association of Dental Traumatology and the Syrian Society for Research and Preventive Dentistry since 2017. He is also a Member of the Reviewer Board of International Journal of Dental Medicine (IJDM), and the Indian Journal of Conservative and Endodontics since 2016.",institutionString:"International University for Science and Technology.",institution:{name:"Islamic University of Science and Technology",country:{name:"India"}}},{id:"42847",title:"Dr.",name:"Belma",middleName:null,surname:"Işik Aslan",slug:"belma-isik-aslan",fullName:"Belma Işik Aslan",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/42847/images/system/42847.jpg",biography:"Dr. Belma IşIk Aslan was born in 1976 in Ankara-TURKEY. After graduating from TED Ankara College in 1994, she attended to Gazi University, Faculty of Dentistry in Ankara. She completed her PhD in orthodontic education at Gazi University between 1999-2005. Dr. Işık Aslan stayed at the Providence Hospital Craniofacial Institude and Reconstructive Surgery in Michigan, USA for three months as an observer. She worked as a specialist doctor at Gazi University, Dentistry Faculty, Department of Orthodontics between 2005-2014. She was appointed as associate professor in January, 2014 and as professor in 2021. Dr. Işık Aslan still works as an instructor at the same faculty. She has published a total of 35 articles, 10 book chapters, 39 conference proceedings both internationally and nationally. Also she was the academic editor of the international book 'Current Advances in Orthodontics'. She is a member of the Turkish Orthodontic Society and Turkish Cleft Lip and Palate Society. She is married and has 2 children. Her knowledge of English is at an advanced level.",institutionString:"Gazi University Dentistry Faculty Department of Orthodontics",institution:null},{id:"178412",title:"Associate Prof.",name:"Guhan",middleName:null,surname:"Dergin",slug:"guhan-dergin",fullName:"Guhan Dergin",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/178412/images/6954_n.jpg",biography:"Assoc. Prof. Dr. Gühan Dergin was born in 1973 in Izmit. He graduated from Marmara University Faculty of Dentistry in 1999. He completed his specialty of OMFS surgery in Marmara University Faculty of Dentistry and obtained his PhD degree in 2006. In 2005, he was invited as a visiting doctor in the Oral and Maxillofacial Surgery Department of the University of North Carolina, USA, where he went on a scholarship. Dr. Dergin still continues his academic career as an associate professor in Marmara University Faculty of Dentistry. He has many articles in international and national scientific journals and chapters in books.",institutionString:null,institution:{name:"Marmara University",country:{name:"Turkey"}}},{id:"178414",title:"Prof.",name:"Yusuf",middleName:null,surname:"Emes",slug:"yusuf-emes",fullName:"Yusuf Emes",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/178414/images/6953_n.jpg",biography:"Born in Istanbul in 1974, Dr. Emes graduated from Istanbul University Faculty of Dentistry in 1997 and completed his PhD degree in Istanbul University faculty of Dentistry Department of Oral and Maxillofacial Surgery in 2005. He has papers published in international and national scientific journals, including research articles on implantology, oroantral fistulas, odontogenic cysts, and temporomandibular disorders. Dr. Emes is currently working as a full-time academic staff in Istanbul University faculty of Dentistry Department of Oral and Maxillofacial Surgery.",institutionString:null,institution:{name:"Istanbul University",country:{name:"Turkey"}}},{id:"192229",title:"Ph.D.",name:"Ana Luiza",middleName:null,surname:"De Carvalho Felippini",slug:"ana-luiza-de-carvalho-felippini",fullName:"Ana Luiza De Carvalho Felippini",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/192229/images/system/192229.jpg",biography:null,institutionString:"University of São Paulo",institution:{name:"University of Sao Paulo",country:{name:"Brazil"}}},{id:"256851",title:"Prof.",name:"Ayşe",middleName:null,surname:"Gülşen",slug:"ayse-gulsen",fullName:"Ayşe Gülşen",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/256851/images/9696_n.jpg",biography:"Dr. Ayşe Gülşen graduated in 1990 from Faculty of Dentistry, University of Ankara and did a postgraduate program at University of Gazi. \nShe worked as an observer and research assistant in Craniofacial Surgery Departments in New York, Providence Hospital in Michigan and Chang Gung Memorial Hospital in Taiwan. \nShe works as Craniofacial Orthodontist in Department of Aesthetic, Plastic and Reconstructive Surgery, Faculty of Medicine, University of Gazi, Ankara Turkey since 2004.",institutionString:"Univeristy of Gazi",institution:null},{id:"255366",title:"Prof.",name:"Tosun",middleName:null,surname:"Tosun",slug:"tosun-tosun",fullName:"Tosun Tosun",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/255366/images/7347_n.jpg",biography:"Graduated at the Faculty of Dentistry, University of Istanbul, Turkey in 1989;\nVisitor Assistant at the University of Padua, Italy and Branemark Osseointegration Center of Treviso, Italy between 1993-94;\nPhD thesis on oral implantology in University of Istanbul and was awarded the academic title “Dr.med.dent.”, 1997;\nHe was awarded the academic title “Doç.Dr.” (Associated Professor) in 2003;\nProficiency in Botulinum Toxin Applications, Reading-UK in 2009;\nMastership, RWTH Certificate in Laser Therapy in Dentistry, AALZ-Aachen University, Germany 2009-11;\nMaster of Science (MSc) in Laser Dentistry, University of Genoa, Italy 2013-14.\n\nDr.Tosun worked as Research Assistant in the Department of Oral Implantology, Faculty of Dentistry, University of Istanbul between 1990-2002. \nHe worked part-time as Consultant surgeon in Harvard Medical International Hospitals and John Hopkins Medicine, Istanbul between years 2007-09.\u2028He was contract Professor in the Department of Surgical and Diagnostic Sciences (DI.S.C.), Medical School, University of Genova, Italy between years 2011-16. \nSince 2015 he is visiting Professor at Medical School, University of Plovdiv, Bulgaria. \nCurrently he is Associated Prof.Dr. at the Dental School, Oral Surgery Dept., Istanbul Aydin University and since 2003 he works in his own private clinic in Istanbul, Turkey.\u2028\nDr.Tosun is reviewer in journal ‘Laser in Medical Sciences’, reviewer in journal ‘Folia Medica\\', a Fellow of the International Team for Implantology, Clinical Lecturer of DGZI German Association of Oral Implantology, Expert Lecturer of Laser&Health Academy, Country Representative of World Federation for Laser Dentistry, member of European Federation of Periodontology, member of Academy of Laser Dentistry. Dr.Tosun presents papers in international and national congresses and has scientific publications in international and national journals. He speaks english, spanish, italian and french.",institutionString:null,institution:{name:"Istanbul Aydın University",country:{name:"Turkey"}}},{id:"171887",title:"Prof.",name:"Zühre",middleName:null,surname:"Akarslan",slug:"zuhre-akarslan",fullName:"Zühre Akarslan",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/171887/images/system/171887.jpg",biography:"Zühre Akarslan was born in 1977 in Cyprus. She graduated from Gazi University Faculty of Dentistry, Ankara, Turkey in 2000. \r\nLater she received her Ph.D. degree from the Oral Diagnosis and Radiology Department; which was recently renamed as Oral and Dentomaxillofacial Radiology, from the same university. \r\nShe is working as a full-time Associate Professor and is a lecturer and an academic researcher. \r\nHer expertise areas are dental caries, cancer, dental fear and anxiety, gag reflex in dentistry, oral medicine, and dentomaxillofacial radiology.",institutionString:"Gazi University",institution:{name:"Gazi University",country:{name:"Turkey"}}},{id:"256417",title:"Associate Prof.",name:"Sanaz",middleName:null,surname:"Sadry",slug:"sanaz-sadry",fullName:"Sanaz Sadry",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/256417/images/8106_n.jpg",biography:null,institutionString:null,institution:null},{id:"272237",title:"Dr.",name:"Pinar",middleName:"Kiymet",surname:"Karataban",slug:"pinar-karataban",fullName:"Pinar Karataban",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/272237/images/8911_n.png",biography:"Assist.Prof.Dr.Pınar Kıymet Karataban, DDS PhD \n\nDr.Pınar Kıymet Karataban was born in Istanbul in 1975. After her graduation from Marmara University Faculty of Dentistry in 1998 she started her PhD in Paediatric Dentistry focused on children with special needs; mainly children with Cerebral Palsy. She finished her pHD thesis entitled \\'Investigation of occlusion via cast analysis and evaluation of dental caries prevalance, periodontal status and muscle dysfunctions in children with cerebral palsy” in 2008. She got her Assist. Proffessor degree in Istanbul Aydın University Paediatric Dentistry Department in 2015-2018. ın 2019 she started her new career in Bahcesehir University, Istanbul as Head of Department of Pediatric Dentistry. In 2020 she was accepted to BAU International University, Batumi as Professor of Pediatric Dentistry. She’s a lecturer in the same university meanwhile working part-time in private practice in Ege Dental Studio (https://www.egedisklinigi.com/) a multidisciplinary dental clinic in Istanbul. Her main interests are paleodontology, ancient and contemporary dentistry, oral microbiology, cerebral palsy and special care dentistry. She has national and international publications, scientific reports and is a member of IAPO (International Association for Paleodontology), IADH (International Association of Disability and Oral Health) and EAPD (European Association of Pediatric Dentistry).",institutionString:null,institution:null},{id:"202198",title:"Dr.",name:"Buket",middleName:null,surname:"Aybar",slug:"buket-aybar",fullName:"Buket Aybar",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/202198/images/6955_n.jpg",biography:"Buket Aybar, DDS, PhD, was born in 1971. She graduated from Istanbul University, Faculty of Dentistry, in 1992 and completed her PhD degree on Oral and Maxillofacial Surgery in Istanbul University in 1997.\nDr. Aybar is currently a full-time professor in Istanbul University, Faculty of Dentistry Department of Oral and Maxillofacial Surgery. She has teaching responsibilities in graduate and postgraduate programs. Her clinical practice includes mainly dentoalveolar surgery.\nHer topics of interest are biomaterials science and cell culture studies. She has many articles in international and national scientific journals and chapters in books; she also has participated in several scientific projects supported by Istanbul University Research fund.",institutionString:null,institution:null},{id:"260116",title:"Dr.",name:"Mehmet",middleName:null,surname:"Yaltirik",slug:"mehmet-yaltirik",fullName:"Mehmet Yaltirik",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/260116/images/7413_n.jpg",biography:"Birth Date 25.09.1965\r\nBirth Place Adana- Turkey\r\nSex Male\r\nMarrial Status Bachelor\r\nDriving License Acquired\r\nMother Tongue Turkish\r\n\r\nAddress:\r\nWork:University of Istanbul,Faculty of Dentistry, Department of Oral Surgery and Oral Medicine 34093 Capa,Istanbul- TURKIYE",institutionString:null,institution:null},{id:"172009",title:"Dr.",name:"Fatma Deniz",middleName:null,surname:"Uzuner",slug:"fatma-deniz-uzuner",fullName:"Fatma Deniz Uzuner",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/172009/images/7122_n.jpg",biography:"Dr. Deniz Uzuner was born in 1969 in Kocaeli-TURKEY. After graduating from TED Ankara College in 1986, she attended the Hacettepe University, Faculty of Dentistry in Ankara. \nIn 1993 she attended the Gazi University, Faculty of Dentistry, Department of Orthodontics for her PhD education. After finishing the PhD education, she worked as orthodontist in Ankara Dental Hospital under the Turkish Government, Ministry of Health and in a special Orthodontic Clinic till 2011. Between 2011 and 2016, Dr. Deniz Uzuner worked as a specialist in the Department of Orthodontics, Faculty of Dentistry, Gazi University in Ankara/Turkey. In 2016, she was appointed associate professor. Dr. Deniz Uzuner has authored 23 Journal Papers, 3 Book Chapters and has had 39 oral/poster presentations. She is a member of the Turkish Orthodontic Society. Her knowledge of English is at an advanced level.",institutionString:null,institution:null},{id:"332914",title:"Dr.",name:"Muhammad Saad",middleName:null,surname:"Shaikh",slug:"muhammad-saad-shaikh",fullName:"Muhammad Saad Shaikh",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Jinnah Sindh Medical University",country:{name:"Pakistan"}}},{id:"315775",title:"Dr.",name:"Feng",middleName:null,surname:"Luo",slug:"feng-luo",fullName:"Feng Luo",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Sichuan University",country:{name:"China"}}},{id:"423519",title:"Dr.",name:"Sizakele",middleName:null,surname:"Ngwenya",slug:"sizakele-ngwenya",fullName:"Sizakele Ngwenya",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"University of the Witwatersrand",country:{name:"South Africa"}}},{id:"419270",title:"Dr.",name:"Ann",middleName:null,surname:"Chianchitlert",slug:"ann-chianchitlert",fullName:"Ann Chianchitlert",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Walailak University",country:{name:"Thailand"}}},{id:"419271",title:"Dr.",name:"Diane",middleName:null,surname:"Selvido",slug:"diane-selvido",fullName:"Diane Selvido",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Walailak University",country:{name:"Thailand"}}},{id:"419272",title:"Dr.",name:"Irin",middleName:null,surname:"Sirisoontorn",slug:"irin-sirisoontorn",fullName:"Irin Sirisoontorn",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Walailak University",country:{name:"Thailand"}}},{id:"355660",title:"Dr.",name:"Anitha",middleName:null,surname:"Mani",slug:"anitha-mani",fullName:"Anitha Mani",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"SRM Dental College",country:{name:"India"}}},{id:"355612",title:"Dr.",name:"Janani",middleName:null,surname:"Karthikeyan",slug:"janani-karthikeyan",fullName:"Janani Karthikeyan",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"SRM Dental College",country:{name:"India"}}},{id:"334400",title:"Dr.",name:"Suvetha",middleName:null,surname:"Siva",slug:"suvetha-siva",fullName:"Suvetha Siva",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"SRM Dental College",country:{name:"India"}}}]}},subseries:{item:{id:"18",type:"subseries",title:"Proteomics",keywords:"Mono- and Two-Dimensional Gel Electrophoresis (1-and 2-DE), Liquid Chromatography (LC), Mass Spectrometry/Tandem Mass Spectrometry (MS; MS/MS), Proteins",scope:"With the recognition that the human genome cannot provide answers to the etiology of a disorder, changes in the proteins expressed by a genome became a focus in research. Thus proteomics, an area of research that detects all protein forms expressed in an organism, including splice isoforms and post-translational modifications, is more suitable than genomics for a comprehensive understanding of the biochemical processes that govern life. The most common proteomics applications are currently in the clinical field for the identification, in a variety of biological matrices, of biomarkers for diagnosis and therapeutic intervention of disorders. From the comparison of proteomic profiles of control and disease or different physiological states, which may emerge, changes in protein expression can provide new insights into the roles played by some proteins in human pathologies. Understanding how proteins function and interact with each other is another goal of proteomics that makes this approach even more intriguing. Specialized technology and expertise are required to assess the proteome of any biological sample. Currently, proteomics relies mainly on mass spectrometry (MS) combined with electrophoretic (1 or 2-DE-MS) and/or chromatographic techniques (LC-MS/MS). MS is an excellent tool that has gained popularity in proteomics because of its ability to gather a complex body of information such as cataloging protein expression, identifying protein modification sites, and defining protein interactions. The Proteomics topic aims to attract contributions on all aspects of MS-based proteomics that, by pushing the boundaries of MS capabilities, may address biological problems that have not been resolved yet.",coverUrl:"https://cdn.intechopen.com/series_topics/covers/18.jpg",hasOnlineFirst:!0,hasPublishedBooks:!0,annualVolume:11414,editor:{id:"200689",title:"Prof.",name:"Paolo",middleName:null,surname:"Iadarola",slug:"paolo-iadarola",fullName:"Paolo Iadarola",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bSCl8QAG/Profile_Picture_1623568118342",biography:"Paolo Iadarola graduated with a degree in Chemistry from the University of Pavia (Italy) in July 1972. He then worked as an Assistant Professor at the Faculty of Science of the same University until 1984. In 1985, Prof. Iadarola became Associate Professor at the Department of Biology and Biotechnologies of the University of Pavia and retired in October 2017. Since then, he has been working as an Adjunct Professor in the same Department at the University of Pavia. His research activity during the first years was primarily focused on the purification and structural characterization of enzymes from animal and plant sources. During this period, Prof. Iadarola familiarized himself with the conventional techniques used in column chromatography, spectrophotometry, manual Edman degradation, and electrophoresis). Since 1995, he has been working on: i) the determination in biological fluids (serum, urine, bronchoalveolar lavage, sputum) of proteolytic activities involved in the degradation processes of connective tissue matrix, and ii) on the identification of biological markers of lung diseases. In this context, he has developed and validated new methodologies (e.g., Capillary Electrophoresis coupled to Laser-Induced Fluorescence, CE-LIF) whose application enabled him to determine both the amounts of biochemical markers (Desmosines) in urine/serum of patients affected by Chronic Obstructive Pulmonary Disease (COPD) and the activity of proteolytic enzymes (Human Neutrophil Elastase, Cathepsin G, Pseudomonas aeruginosa elastase) in sputa of these patients. More recently, Prof. Iadarola was involved in developing techniques such as two-dimensional electrophoresis coupled to liquid chromatography/mass spectrometry (2DE-LC/MS) for the proteomic analysis of biological fluids aimed at the identification of potential biomarkers of different lung diseases. He is the author of about 150 publications (According to Scopus: H-Index: 23; Total citations: 1568- According to WOS: H-Index: 20; Total Citations: 1296) of peer-reviewed international journals. He is a Consultant Reviewer for several journals, including the Journal of Chromatography A, Journal of Chromatography B, Plos ONE, Proteomes, International Journal of Molecular Science, Biotech, Electrophoresis, and others. He is also Associate Editor of Biotech.",institutionString:null,institution:{name:"University of Pavia",institutionURL:null,country:{name:"Italy"}}},editorTwo:{id:"201414",title:"Dr.",name:"Simona",middleName:null,surname:"Viglio",slug:"simona-viglio",fullName:"Simona Viglio",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRKDHQA4/Profile_Picture_1630402531487",biography:"Simona Viglio is an Associate Professor of Biochemistry at the Department of Molecular Medicine at the University of Pavia. She has been working since 1995 on the determination of proteolytic enzymes involved in the degradation process of connective tissue matrix and on the identification of biological markers of lung diseases. She gained considerable experience in developing and validating new methodologies whose applications allowed her to determine both the amount of biomarkers (Desmosine and Isodesmosine) in the urine of patients affected by COPD, and the activity of proteolytic enzymes (HNE, Cathepsin G, Pseudomonas aeruginosa elastase) in the sputa of these patients. Simona Viglio was also involved in research dealing with the supplementation of amino acids in patients with brain injury and chronic heart failure. She is presently engaged in the development of 2-DE and LC-MS techniques for the study of proteomics in biological fluids. The aim of this research is the identification of potential biomarkers of lung diseases. 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