Samples collection sites and their eco-geographic characteristics.
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These books synthesize perspectives of renowned scientists from the world’s most prestigious institutions - from Fukushima Renewable Energy Institute in Japan to Stanford University in the United States, including Columbia University (US), University of Sidney (AU), University of Miami (USA), Cardiff University (UK), and many others.
\\n\\nThis collaboration embodied the true essence of Open Access by simplifying the approach to OA publishing for Academic editors and authors who contributed their research and allowed the new research to be made available free and open to anyone anywhere in the world.
\\n\\nTo celebrate the 50 books published, we have gathered them at one location - just one click away, so that you can easily browse the subjects of your interest, download the content directly, share it or read online.
\\n\\n\\n\\n\\n"}]',published:!0,mainMedia:null},components:[{type:"htmlEditorComponent",content:'
IntechOpen and Knowledge Unlatched formed a partnership to support researchers working in engineering sciences by enabling an easier approach to publishing Open Access content. Using the Knowledge Unlatched crowdfunding model to raise the publishing costs through libraries around the world, Open Access Publishing Fee (OAPF) was not required from the authors.
\n\nInitially, the partnership supported engineering research, but it soon grew to include physical and life sciences, attracting more researchers to the advantages of Open Access publishing.
\n\n\n\nThese books synthesize perspectives of renowned scientists from the world’s most prestigious institutions - from Fukushima Renewable Energy Institute in Japan to Stanford University in the United States, including Columbia University (US), University of Sidney (AU), University of Miami (USA), Cardiff University (UK), and many others.
\n\nThis collaboration embodied the true essence of Open Access by simplifying the approach to OA publishing for Academic editors and authors who contributed their research and allowed the new research to be made available free and open to anyone anywhere in the world.
\n\nTo celebrate the 50 books published, we have gathered them at one location - just one click away, so that you can easily browse the subjects of your interest, download the content directly, share it or read online.
\n\n\n\n\n'}],latestNews:[{slug:"webinar-introduction-to-open-science-wednesday-18-may-1-pm-cest-20220518",title:"Webinar: Introduction to Open Science | Wednesday 18 May, 1 PM CEST"},{slug:"step-in-the-right-direction-intechopen-launches-a-portfolio-of-open-science-journals-20220414",title:"Step in the Right Direction: IntechOpen Launches a Portfolio of Open Science Journals"},{slug:"let-s-meet-at-london-book-fair-5-7-april-2022-olympia-london-20220321",title:"Let’s meet at London Book Fair, 5-7 April 2022, Olympia London"},{slug:"50-books-published-as-part-of-intechopen-and-knowledge-unlatched-ku-collaboration-20220316",title:"50 Books published as part of IntechOpen and Knowledge Unlatched (KU) Collaboration"},{slug:"intechopen-joins-the-united-nations-sustainable-development-goals-publishers-compact-20221702",title:"IntechOpen joins the United Nations Sustainable Development Goals Publishers Compact"},{slug:"intechopen-signs-exclusive-representation-agreement-with-lsr-libros-servicios-y-representaciones-s-a-de-c-v-20211123",title:"IntechOpen Signs Exclusive Representation Agreement with LSR Libros Servicios y Representaciones S.A. de C.V"},{slug:"intechopen-expands-partnership-with-research4life-20211110",title:"IntechOpen Expands Partnership with Research4Life"},{slug:"introducing-intechopen-book-series-a-new-publishing-format-for-oa-books-20210915",title:"Introducing IntechOpen Book Series - A New Publishing Format for OA Books"}]},book:{item:{type:"book",id:"6961",leadTitle:null,fullTitle:"Alopecia",title:"Alopecia",subtitle:null,reviewType:"peer-reviewed",abstract:"Being the editor of the book Alopecia, I feel delighted to work with the world-leading publisher IntechOpen Publisher. The current book has chapters emphasizing a variety of alopecias. The administration of newer drugs may treat hair loss by a variety of mechanisms. All the clinical variants of alopecias are discussed in detail. The book will help dermatologists, students, hair transplant surgeons, and physicians related to hair loss problems, giving them the opportunity to understand basic pathophysiological, clinical, and medical management options. The basic idea of the book is to diagnose alopecia correctly.",isbn:"978-1-78984-344-6",printIsbn:"978-1-78984-343-9",pdfIsbn:"978-1-83881-747-3",doi:"10.5772/intechopen.73642",price:119,priceEur:129,priceUsd:155,slug:"alopecia",numberOfPages:148,isOpenForSubmission:!1,isInWos:1,isInBkci:!1,hash:"211055d552abe032133f7281ea2b13dd",bookSignature:"Muhammad Ahmad",publishedDate:"October 31st 2018",coverURL:"https://cdn.intechopen.com/books/images_new/6961.jpg",numberOfDownloads:9056,numberOfWosCitations:6,numberOfCrossrefCitations:3,numberOfCrossrefCitationsByBook:1,numberOfDimensionsCitations:9,numberOfDimensionsCitationsByBook:1,hasAltmetrics:1,numberOfTotalCitations:18,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"January 19th 2018",dateEndSecondStepPublish:"February 9th 2018",dateEndThirdStepPublish:"April 10th 2018",dateEndFourthStepPublish:"June 29th 2018",dateEndFifthStepPublish:"August 28th 2018",currentStepOfPublishingProcess:5,indexedIn:"1,2,3,4,5,6",editedByType:"Edited by",kuFlag:!1,featuredMarkup:null,editors:[{id:"204257",title:"Dr.",name:"Muhammad",middleName:null,surname:"Ahmad",slug:"muhammad-ahmad",fullName:"Muhammad Ahmad",profilePictureURL:"https://mts.intechopen.com/storage/users/204257/images/system/204257.jpeg",biography:"Dr. Muhammad Ahmad is the only surgeon in history to develop various new classifications regarding different aspects of hair\nrestoration. These include a new classification system for hair loss, Ahmad’s NPRT system; a new classification for scalp hair,\nLGMA classification; and a comprehensive classification of hair transection. He has also published Ahmad’s Cosmetic Surgery\nScar Scale and Ahmad’s Hair Transplant Assessment Scale. He is the only Pakistani plastic surgeon to receive the Merit Award from the Australian and New Zealand Burns Association (2004). He was also awarded first prize at the annual meeting of the Asian Association of Hair Restoration Surgeons (AAHRS), Bangkok, Thailand, in 2017. In 2018, he was awarded a Lifetime Achievement Award from the Pakistan Society of Hair Restoration Surgery. Dr. Ahmad has more than 150 publications in national and international journals. He is also editor and reviewer of many international journals. He is among few ABHRS Diplomates who have more than 100 publications.",institutionString:"Aesthetic Plastic Surgery and Hair Transplant Institute",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"0",totalChapterViews:"0",totalEditedBooks:"2",institution:null}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,coeditorOne:null,coeditorTwo:null,coeditorThree:null,coeditorFour:null,coeditorFive:null,topics:[{id:"1003",title:"Trichology",slug:"trichology"}],chapters:[{id:"62397",title:"Evaluation of Patients with Alopecia",doi:"10.5772/intechopen.78639",slug:"evaluation-of-patients-with-alopecia",totalDownloads:1858,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:1,abstract:"This chapter outlines the clinical approaches for alopecic patients that are reliable in practice. We discuss three different categories of hair evaluation options: invasive methods (biopsy), semi-invasive methods (trichogram) and noninvasive methods. Besides describing the current status of diagnosis and quantification of alopecia, the chapter provides an objective assessment of these investigation tools: detailed medical history collection by structured interview and questionnaires, clinical examination of the scalp and other hair-bearing areas, laboratory investigations, assessment of hair loss distribution (patterned/diffuse/focal), dermoscopic evaluation, assessment of alopecia severity (by pull test, hair part width, counting hair test), common scales for hair loss staging, photography of alopecic areas, biopsy, trichogram, unit area trichogram, tug test, hair mount and microscopic evaluation, electron microscopy, hair card test, hair weight determination, hair densitometry, mechanical test of hair quality and computed hair analysis. Unfortunately, the disadvantages of most of these methods generate a lack of use in clinical practice, leading to few reliable evaluation methods for patients suffering from alopecia. We underline the necessity of easy, refined and precise evaluation tools for the assessment of alopecia patients.",signatures:"Meda Sandra Orasan, Andrei Coneac and Iulia Ioana Roman",downloadPdfUrl:"/chapter/pdf-download/62397",previewPdfUrl:"/chapter/pdf-preview/62397",authors:[{id:"202125",title:"Dr.",name:"Meda",surname:"Orasan",slug:"meda-orasan",fullName:"Meda Orasan"},{id:"205669",title:"Dr.",name:"Andrei",surname:"Coneac",slug:"andrei-coneac",fullName:"Andrei Coneac"},{id:"255002",title:"Dr.",name:"Iulia Ioana",surname:"Roman",slug:"iulia-ioana-roman",fullName:"Iulia Ioana Roman"}],corrections:null},{id:"62782",title:"Perspectives of Alopecia behind the Regulation of Foxn1 Gene Exposes the Human Nude Phenotype",doi:"10.5772/intechopen.79797",slug:"perspectives-of-alopecia-behind-the-regulation-of-foxn1-gene-exposes-the-human-nude-phenotype",totalDownloads:1080,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"The hair follicle (HF) is remarkable for its dynamic structure and one of the most prominent mini organs of the skin. The most visible end product formed by the hair follicle known as “hair shaft”- a tissue with a highly keratinized protein. Therefore, alopecia or baldness issue largely depends on the equilibrium between keratinocyte growth and differentiation of the HF. However, molecular nature in mice, rats, and humans, loss of transcription factor Foxn1 the keratinization processes is significantly impaired. Hence, this nude gene lack of function makes very similar hair pattern baldness in both human and nude mice (Foxn1nu/Foxn1nu). Thus highlighting the usefulness of mouse mutants and mouse genomics as a research tool for better molecular controls of human hair biology. To enhance research efforts of Foxn1 target gene regulation and the pharmacological manipulation of the nude phenotype are important open questions for promising research strategies related with Foxn1 biology. Taken together these issues may open the discovery of the investigative dermatology that promises the controls of epithelial differentiation in mammalian skin.",signatures:"Shahnaz Begum, Md Jamil Hossain, Li Juan Gu and Chang Keun\nSung",downloadPdfUrl:"/chapter/pdf-download/62782",previewPdfUrl:"/chapter/pdf-preview/62782",authors:[{id:"245760",title:"Dr.",name:"Shahnaz",surname:"Begum",slug:"shahnaz-begum",fullName:"Shahnaz Begum"},{id:"257315",title:"Dr.",name:"Li",surname:"Juan Gu",slug:"li-juan-gu",fullName:"Li Juan Gu"},{id:"257317",title:"Dr.",name:"Md Jamil",surname:"Hossain",slug:"md-jamil-hossain",fullName:"Md Jamil Hossain"},{id:"257318",title:"Dr.",name:"Chang Keun",surname:"Sung",slug:"chang-keun-sung",fullName:"Chang Keun Sung"}],corrections:null},{id:"63046",title:"Medical Treatment of Alopecia",doi:"10.5772/intechopen.79796",slug:"medical-treatment-of-alopecia",totalDownloads:1099,totalCrossrefCites:0,totalDimensionsCites:1,hasAltmetrics:0,abstract:"Alopecia means partial or complete loss of hair from a part of the body where it exists naturally. It affects both men and women, and its treatment depends upon its cause, age of onset, and clinical presentation. It is divided into scarring and non-scarring alopecia. Scarring alopecia includes pseudopelade of Brocq, central centrifugal cicatricial alopecia, folliculitis decalvans, acne keloidalis nuchae, lichen planopilaris, frontal fibrosing alopecia and discoid lupus erythematosus, traumatic i.e., injury, radiation and post-operative scarring alopecia and certain neoplasms. Common causes of non-scarring alopecia are androgenic alopecia, alopecia areata, telogen effluvium, anagen effluvium, trichotillomania, traction alopecia, pressure-induced alopecia, alopecia due to iron deficiency, thyroid disease, and polycystic ovary syndrome. Topical remedies available are minoxidil 2 and 5%, topical & intralesional steroids, topical sensitization, anthralin, retinoids, tacrolimus, garlic, ketoconazole and prostaglandin analogs. Among systemic treatments, finasteride, steroids, immunosuppressant like azathioprine, methotrexate, sulfasalazine, zinc sulfate and iron are widely accepted. The phototherapies, photo-chemotherapies, platelet rich plasma (PRP) therapy, pharmacogenetics and hair transplant are new remedies for alopecia. It is concluded that minoxidil, finasteride, PRP, and hair transplant are the most widely being used modalities for alopecia.",signatures:"Abdul Aziz Khalid",downloadPdfUrl:"/chapter/pdf-download/63046",previewPdfUrl:"/chapter/pdf-preview/63046",authors:[{id:"241090",title:"Dr.",name:"Abdul Aziz",surname:"Khalid",slug:"abdul-aziz-khalid",fullName:"Abdul Aziz Khalid"}],corrections:null},{id:"63066",title:"Pharmacological Treatment of Alopecia",doi:"10.5772/intechopen.79656",slug:"pharmacological-treatment-of-alopecia",totalDownloads:1415,totalCrossrefCites:1,totalDimensionsCites:2,hasAltmetrics:0,abstract:"In this chapter, we will explore non-surgical treatments of alopecia. Unlike many other areas of medicine, pharmacological treatments for alopecia are relatively new. There are only two treatments which are approved by the Food and Drug Administration (FDA); the rest are drugs developed for other indications which have gained popular off-label use to promote hair growth. The reasons for this are many, including the designation of alopecia by the FDA as a cosmetic disease. This designation has restricted alopecia development programs to compounds with virtually no side effects. Unfortunately, it has also led to off-label use of far more dangerous compounds as alopecia treatments, without the benefit of controlled trials. There is a growing recognition that alopecia, particularly alopecia areata and chemotherapy-induced alopecia, are disorders which significantly alter the quality of life, similar to acne vulgaris and psoriasis, and merit treatment accordingly. There have also been several recent advances in our understanding of the hair cycle, revealing new targets for developing alopecia therapies. As a result, there is a more robust slate of programs for developing new pharmacological treatments for alopecia. In this chapter, we will review current pharmacological treatments for alopecia and selected treatments under development (i.e., those with significant preclinical or clinical data which have appeared in the published literature).",signatures:"Robert Gensure",downloadPdfUrl:"/chapter/pdf-download/63066",previewPdfUrl:"/chapter/pdf-preview/63066",authors:[{id:"16515",title:"Dr.",name:"Robert",surname:"Gensure",slug:"robert-gensure",fullName:"Robert Gensure"}],corrections:null},{id:"63719",title:"Cicatricial Alopecia",doi:"10.5772/intechopen.78971",slug:"cicatricial-alopecia",totalDownloads:1218,totalCrossrefCites:1,totalDimensionsCites:1,hasAltmetrics:0,abstract:"Cicatricial alopecia represents a group of disorders sharing a final pathway of destruction followed by replacement with fibrous tissue of the hair follicle unit. Cicatricial alopecia is classified into two categories, namely primary cicatricial alopecia, in which the hair follicle is the sole target of a progressive inflammatory process in a group of diverse skin or systemic diseases, and secondary cicatricial alopecia, referring to the hair follicle destruction as a result of a nonspecific disruption of the dermis. Permanent hair loss may also occur in the late phases of some nonscarring alopecias that are called “biphasic alopecias.” Based on the pathological characteristics, the lesions of primary cicatricial alopecia are divided into lymphocyte-predominant subgroup, neutrophil-predominant subgroup, or mixed subgroup. In principle, the primary goal of the treatment aims to attenuate the progression of the inflammatory and the scarring processes at the earliest phase of the disease. In clinical practice, the lymphocyte-predominant lesions are treated with immunosuppressive agents, whereas the neutrophil-predominant lesions are treated with antimicrobials or dapsone. As the efficacy of medication treatment against the cicatricial alopecia varies significantly, autologous hair transplantation is recommended to patients who have a relatively stable primary or a secondary cicatricial alopecia.",signatures:"Yingjun Su, Qing Yang, Wenjie Dou, Ping Xue, Xianjie Ma, Xianhui\nZeng, Lei Wang and Chiyu Jia",downloadPdfUrl:"/chapter/pdf-download/63719",previewPdfUrl:"/chapter/pdf-preview/63719",authors:[{id:"243967",title:"Prof.",name:"Yingjun",surname:"Su",slug:"yingjun-su",fullName:"Yingjun Su"},{id:"254901",title:"Mr.",name:"Qing",surname:"Yang",slug:"qing-yang",fullName:"Qing Yang"},{id:"254902",title:"Dr.",name:"Wenjie",surname:"Dou",slug:"wenjie-dou",fullName:"Wenjie Dou"},{id:"254903",title:"Dr.",name:"Ping",surname:"Xue",slug:"ping-xue",fullName:"Ping Xue"},{id:"254904",title:"BSc.",name:"Xianhui",surname:"Zeng",slug:"xianhui-zeng",fullName:"Xianhui Zeng"},{id:"254905",title:"Prof.",name:"Xianjie",surname:"Ma",slug:"xianjie-ma",fullName:"Xianjie Ma"}],corrections:null},{id:"62733",title:"Ethosomes: An Exciting and Promising Alcoholic Carrier System for Treating Androgenic Alopecia",doi:"10.5772/intechopen.79807",slug:"ethosomes-an-exciting-and-promising-alcoholic-carrier-system-for-treating-androgenic-alopecia",totalDownloads:1063,totalCrossrefCites:1,totalDimensionsCites:5,hasAltmetrics:0,abstract:"Androgenetic alopecia (male-pattern hair loss) is characterized by the deposition of dihydrotestosterone at the pilosebaceous unit of the scalp. Oral administration of drugs (like finasteride) which can reverse androgenic alopecia causes undesired effects to the body. Targeting these drugs directly to the pilosebaceous unit of the scalp will enhance the pharmacological response at the desired site by reducing undesired systemic side effects. This chapter discusses about ethosomes, a specially tailored ethanolic vesicular carriers which can efficiently deliver various drugs with different physicochemical properties to and through the skin. The unique characteristics of the ethosomal carriers, their composition, preparation methods, and the mechanism of permeation, safety, and practical experience (finasteride and herbal extracts) have been discussed in detail.",signatures:"Veintramuthu Sankar, Santhanam Ramesh and Karthik Siram",downloadPdfUrl:"/chapter/pdf-download/62733",previewPdfUrl:"/chapter/pdf-preview/62733",authors:[{id:"254541",title:"Prof.",name:"Sankar",surname:"Veintramuthu",slug:"sankar-veintramuthu",fullName:"Sankar Veintramuthu"},{id:"260986",title:"Mr.",name:"Karthik",surname:"Siram",slug:"karthik-siram",fullName:"Karthik Siram"},{id:"260991",title:"Dr.",name:"Santhanam",surname:"Ramesh",slug:"santhanam-ramesh",fullName:"Santhanam Ramesh"}],corrections:null},{id:"62079",title:"Anesthesia in Hair Transplantation",doi:"10.5772/intechopen.78352",slug:"anesthesia-in-hair-transplantation",totalDownloads:1324,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"Current hair transplantation techniques require a reliable anesthesia for long periods of time (2 h or more). They demand hemostasis of extended surfaces on wide-awake patients. A combination of anesthetic agents and local vasoconstrictors is needed. We present customary technical characteristics of these procedures as local nerve blocks (supratrochlear nerve, supraorbital nerve, zygomaticotemporal nerve, auriculotemporal nerve, retroauricular nerve, lesser occipital nerve, great occipital nerve) and tumescent field anesthesia. The ordinary drug combinations for premedication and procedure are presented. Special emphasis is done to discuss recommendations to cope with undesirable events that may arise during anesthesia (vasovagal syncope, anesthetic toxicity, anaphylactic and allergic reactions).",signatures:"Wenceslao M. Calonge and Darya Louie",downloadPdfUrl:"/chapter/pdf-download/62079",previewPdfUrl:"/chapter/pdf-preview/62079",authors:[{id:"202013",title:"M.D.",name:"Wenceslao M",surname:"Calonge",slug:"wenceslao-m-calonge",fullName:"Wenceslao M Calonge"},{id:"245121",title:"Dr.",name:"Darya",surname:"Louie",slug:"darya-louie",fullName:"Darya Louie"}],corrections:null}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"},subseries:null,tags:null},relatedBooks:[{type:"book",id:"9066",title:"Wound Healing",subtitle:null,isOpenForSubmission:!1,hash:"a293ecd8c2655a402321dc30e0ffbf9a",slug:"wound-healing",bookSignature:"Muhammad Ahmad",coverURL:"https://cdn.intechopen.com/books/images_new/9066.jpg",editedByType:"Edited by",editors:[{id:"204257",title:"Dr.",name:"Muhammad",surname:"Ahmad",slug:"muhammad-ahmad",fullName:"Muhammad Ahmad"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"3038",title:"Current Genetics in Dermatology",subtitle:null,isOpenForSubmission:!1,hash:"384a276072f522c3aea68f9d2a0dbfd8",slug:"current-genetics-in-dermatology",bookSignature:"Naoki Oiso",coverURL:"https://cdn.intechopen.com/books/images_new/3038.jpg",editedByType:"Edited by",editors:[{id:"32053",title:"Dr.",name:"Naoki",surname:"Oiso",slug:"naoki-oiso",fullName:"Naoki Oiso"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"5461",title:"Hair and Scalp Disorders",subtitle:null,isOpenForSubmission:!1,hash:"87c272cade1ee498e1b4d6051aa8d41e",slug:"hair-and-scalp-disorders",bookSignature:"Zekayi Kutlubay and Server Serdaroglu",coverURL:"https://cdn.intechopen.com/books/images_new/5461.jpg",editedByType:"Edited by",editors:[{id:"64792",title:"Dr.",name:"Zekayi",surname:"Kutlubay",slug:"zekayi-kutlubay",fullName:"Zekayi Kutlubay"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"1591",title:"Infrared Spectroscopy",subtitle:"Materials Science, Engineering and Technology",isOpenForSubmission:!1,hash:"99b4b7b71a8caeb693ed762b40b017f4",slug:"infrared-spectroscopy-materials-science-engineering-and-technology",bookSignature:"Theophile Theophanides",coverURL:"https://cdn.intechopen.com/books/images_new/1591.jpg",editedByType:"Edited by",editors:[{id:"37194",title:"Dr.",name:"Theophile",surname:"Theophanides",slug:"theophile-theophanides",fullName:"Theophile Theophanides"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"3161",title:"Frontiers in Guided Wave Optics and Optoelectronics",subtitle:null,isOpenForSubmission:!1,hash:"deb44e9c99f82bbce1083abea743146c",slug:"frontiers-in-guided-wave-optics-and-optoelectronics",bookSignature:"Bishnu Pal",coverURL:"https://cdn.intechopen.com/books/images_new/3161.jpg",editedByType:"Edited by",editors:[{id:"4782",title:"Prof.",name:"Bishnu",surname:"Pal",slug:"bishnu-pal",fullName:"Bishnu Pal"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"3092",title:"Anopheles mosquitoes",subtitle:"New insights into malaria vectors",isOpenForSubmission:!1,hash:"c9e622485316d5e296288bf24d2b0d64",slug:"anopheles-mosquitoes-new-insights-into-malaria-vectors",bookSignature:"Sylvie Manguin",coverURL:"https://cdn.intechopen.com/books/images_new/3092.jpg",editedByType:"Edited by",editors:[{id:"50017",title:"Prof.",name:"Sylvie",surname:"Manguin",slug:"sylvie-manguin",fullName:"Sylvie Manguin"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"371",title:"Abiotic Stress in Plants",subtitle:"Mechanisms and Adaptations",isOpenForSubmission:!1,hash:"588466f487e307619849d72389178a74",slug:"abiotic-stress-in-plants-mechanisms-and-adaptations",bookSignature:"Arun Shanker and B. 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Residues",doi:"10.5772/intechopen.97762",slug:"total-phenolic-content-and-polyphenolic-profile-of-tunisian-rosemary-em-rosmarinus-officinalis-em-l-",body:'
Herbs and plants have been used for a large range of purposes including medicine, pharmaceuticals, nutrition, food preservation, flavorings, beverages, repellents, fragrances and cosmetics. Since prehistoric times, they were the basis for medicinal therapy until synthetic drugs were developed in the nineteenth century [1, 2]. In recent decades, the use of herbs and plants has been of great interest, as they have been the sources of natural products, commonly named as bioactive compounds, with beneficial activities, namely polyphenols, vitamins, polysaccharides and minerals [3]. Nowadays the use of natural compounds is also increasing around the world due to their mild features and low side effects [4, 5]. Cosmetic preparations from herbal origin are popular among consumers, as these agents are typically non-toxic and possess strong activities [6].
Preliminary studies demonstrated that some herbs extracts are as efficient as the synthetic antioxidants such as butylated hydroxytoluene (BHT) and butylated hydroxyanisole (BHA), which have carcinogenic effects in living organisms [3, 4, 7]. Several studies have indicated that the consumption of natural antioxidant compounds protect cells against the damage of reactive oxygen species such as singlet oxygen, superoxide, peroxyl radicals, hydroxyl radicals and peroxynitrite (Yang et al., 2013) [8]. Recently numerous reports have described antioxidants and compounds with beneficial activities, would be beneficial for healthy life, present in fruits, vegetables, herbs and cereals extracts [3, 9]. For all the described reasons, during the last decade there has been a growing interest in the formulation of new cosmetic, food and pharmaceutical products containing natural compounds with antioxidant activity and other beneficial properties for healthy life.
The evidence for antioxidant effect of spices and herbs in food systems was initially determined by Chipault et
Rosemary (
Rosemary is among the most promising sources for the recovery of essential oils through hydrodistillation and polyphenols. After extracting the essential oils, the residues remaining postdistillation (wastes of hydro-distillation) is considered a natural source of antioxidants with several biological activites [9, 30]. Up to today, their exploitation for polyphenol recovery has been limited. The residues remaining after essential oil recovery currently disposed as waste, could be extracted to obtain natural extracts rich in phenolic compounds and with a high antioxidant activity [34].
This current study was undertaken with the aim to identify and quantify the polyphenolic compounds (polyphenolic profile) and to determine the total phenolic content of the residues (waste of hydro-distillation) of rosemary aerial parts in order to revalorize this plant and to increase the economic values of this valuable products as a source of bioactive molecules with beneficial properties that might confer benefits to human health.
A total of 40 individual samples of wild rosemary shrubs (4 locations with 10 plants/area) from two different bioclimatic areas (Semi-arid superior and lower arid) were randomly collected at the full bloom phenological stage (spring 2017). Voucher specimens of rosemary from every location are deposited at the Herbarium of Laboratory of Aromatic and Medicinal Plants of the Research Group on Rainfed Crops for the Rural Development, Murcia Institute of Agri-Food Research and Development (IMIDA), Murcia, Spain.
Details of collection sites are given in Table 1. Fresh aerial parts of plants were firstly dried at room temperature for ten days and afterwards dried in a forced-air drier at 35°C for 48 h, until they reached a constant weight.
Collection site | Bioclimatic stage | Rainfall mm/year | Temp °C/year | Geographical location | ||
---|---|---|---|---|---|---|
Longitude (N) | Latitude (E) | Altitude (m) | ||||
ElKef-Menzel Salem (KMS) | Semi-arid superior | 446 | 16.2 | 35°51′24.0” | 8°28′34.0” | 995 |
Elkef-Sers (KS) | Semi-arid superior | 441 | 16.9 | 36°4′36.2” | 9°1′21.8” | 887 |
Gafsa-Orbata (GO) | Arid lower | 223 | 19.6 | 34°22′49.8” | 9°3′23.4” | 1165 |
Gafsa-Sened (GS) | Semi-arid superior | 222 | 17.3 | 34°28′1.2” | 9°16′1.2” | 392 |
Samples collection sites and their eco-geographic characteristics.
2,2-Diphenyl-1-picrylhydrazyl (DPPH•), ascorbic acid, potassium persulfate, the Folin–Ciocalteu reagent, gallic acid and high purity standards were purchased from Sigma-Aldrich (Madrid, Spain). Methanol, acetonitrile, formic acid, anhydrous sodium carbonate and sodium acetate were supplied from Scharlau Chemie S.A. (Sentmenat, Spain). Methanol was of HPLC grade and other reagents were of analytical grade.
After extracting the essential oils, the residues remaining postdistillation (wastes of hydro-distillation) is considered a natural source of antioxidants [35, 36]. In order to avoid interferences from the essential oil components, individual plants were firstly distilled in a Clevenger system for 3 h, after this, the oil free distilled plant material was dried in a forced-air drier at 35°C for 48 h (until it reached a constant weight) and then ground to pass through a 2-mm mesh. Dried samples (0.5 g) were extracted using 150 mL of methanol in a Soxhlet extractor (B-811) (Buchi, Flawil, Switzerland), for 2 h under a nitrogen atmosphere. Methanolic extracts (ME) were taken to dryness at 40°C under vacuum conditions in an evaporator system (Syncore Polyvap R-96) (Buchi, Flawil, Switzerland) (Figure 1). The residue was re-dissolved in methanol and made up to 5 mL [37]. The yield of the extracts was expressed in terms of milligrams of dry methanolic extract per gram of dry plant weight. Final extracts were kept in vials at −80°C until their corresponding analyses.
Preparation of methanolic extracts of post-distilled rosemary plant.
Total phenolic contents in the extracts were determined by the Folin–Ciocalteu method [38]. A reaction mixture of 15 μL of methanolic extracts, 1185 μL of distilled water and 75 μL of Folin–Ciocalteu reagent were prepared. A vigorous stirring was performed and 225 μL of a sodium carbonate (20%) were added. The tubes were incubated in the dark for 30 min and then the absorbance was measured at 765 nm and 25°C with a Shimadzu (UV-2401PC, Japan) spectrophotometer. Standard curve was prepared by using different concentrations ranging from 100 to 1000 mg/L of gallic acid. Total phenolic content was expressed as mg gallic acid/g dry extract (mg GAE/g DE). Analyses were done in triplicate.
For the HPLC analysis, a method adapted from Zheng and Wang [1] was performed on a reverse phase ZORBAX SB-C18 column (4.6 x 250 mm, 5 μm pore size, Hewlett Packard, USA) using a guard column (ZORBAX SB-C18 4.6 x 125 mm, 5 μm pore size, Hewlett Packard, USA) at ambient temperature. Extracts were passed through a 0.45 μm filter (Millipore SAS, Molsheim, France) and 20 μL were injected in a Hewlett Packard (Germany) system equipped with a G1311A quaternary pump and G1315A photodiode array UV/Vis detector. The mobile phase was acetonitrile (A) and acidified water containing 0.05% formic acid (B). The gradient was as follows: 0 min, 5% A; 10 min, 15%A; 30 min, 25%A; 35 min, 30%A; 50 min, 55%A; 55 min, 90%A; 57 min, 100% A and then held for 10 min before returning to the initial conditions. The flow rate was 1.0 mL/min and the wavelengths of detection were set at 280 and 330 nm. Identification of the phenolic components was made by comparison of retention times and spectra with those of commercially available standard compounds. For quantification, linear regression models were determined using standard dilution techniques. Phenolic compound contents were expressed in mg per g of dry plant weight.
All data were reported as means ± standard deviation (SD). A one-way ANOVA was carried out to assess for significant differences (significant model was accepted for a p-value <0.05) using the IBM SPSS Statistic Program (v. 25). Next, Fisher’s LSD pairwise comparison was performed on the data.
Residues of the hydro-distillation process of aromatic plants oils had been studied for their contents of a diversity of biologically active compounds including antioxidants such as phenolic acids and flavonoids, that could be employed to increase the shelf life of food [13, 35]. The total phenolic content (TPC) in the post-distilled rosemary extracts ranged from 85.8 to 137.3 mg GAE/g DE (Table 2). Among the studied collection sites, post-distilled plants of GO population had the highest phenolic content (137.3 mg GAE/g DE). GS population had the lowest phenolic content (85.8 mg GAE/g DE). Our result showed higher TPC in comparison with that obtained by Jordan et
Kef Seres | Kef Menzel Salem | Gafsa Orbata | Gafsa Sned | |
---|---|---|---|---|
85.8 ± 28. 4a | 109.1 ± 23.1b | 137.3 ± 15.6c | 87.8 ± 15.0a | |
Salvianic Acid | 1.10 ± 0.13ab | 1.13 ± 0.23ab | 1.21 ± 0.39b | 0.84 ± 0.13a |
Caffeic Acid | 1.00 ± 0.22b | 0.90 ± 0.11bab | 0.74 ± 0.26bab | 0.69 ± 0.27a |
Rosmarinic Acid | 29.91 ± 9.33c | 17.96 ± 3.25ab | 26.02 ± 5.88bc | 16.77 ± 7.59a |
Salvianolic Acid A | 1.76 ± 0.44a | 1.20 ± 0.34a | 2.62 ± 0.84b | 1.32 ± 0.40a |
Luteolin −7-O-Rutinoxide | 0.98 ± 0.40b | 0.74 ± 0.18ab | 0.74 ± 0.37ab | 0.57 ± 0.25a |
Luteolin-7-Glucoronide | 2.56 ± 0.80b | 1.89 ± 0.59ab | 1.15 ± 0.49a | 1.28 ± 0.62ab |
Hesperidin | 10.41 ± 2.79a | 14.0 ± 2.69b | 10.6 ± 2.77ab | 9.85 ± 3.47a |
Luteolin | 0.77 ± 0.10a | 0.97 ± 0.13b | 0.81 ± 0.11ab | 0.78 ± 0.18a |
Apigenin | 0.23 ± 0.05a | 0.24 ± 0.06a | 0.24 ± 0.05a | 0.2 ± 0.05a |
Hispidulin | 0.34 ± 0.05a | 0.48 ± 0.09b | 0.41 ± 0.09ab | 0.37 ± 0.08a |
Cirsimaritin | 1.21 ± 0.58a | 1.53 ± 0.48a | 1.32 ± 0.41a | 1.16 ± 0.42a |
Genkwanin | 3.30 ± 1.33a | 2.51 ± 0.71a | 2.07 ± 0.84a | 2.05 ± 1.22a |
Salvigenin | 1.05 ± 0.31a | 1.13 ± 0.3a | 1.59 ± 0.5b | 1.22 ± 0.37ab |
Rosmadial | 1.83 ± 0.32a | 2.49 ± 0.41bc | 2.68 ± 0.54c | 1.98 ± 057ab |
7-CH3-Rosmanol | 1.45 ± 0.35a | 1.49 ± 0.26a | 3.73 ± 0.75b | 1.12 ± 0.31a |
Carnosol | 26.94 ± 4.86ab | 29.95 ± 3.57b | 43.53 ± 4.18c | 22.36 ± 4.17a |
Carnosic acid | 57.33 ± 22.37a | 54.74 ± 9.24a | 76.36 ± 12.87b | 46.27 ± 12.01a |
12-CH3- Carnosic Acid | 9.60 ± 4.86a | 10.60 ± 4.00a | 16.70 ± 5.84b | 10.6 ± 2.78a |
Total phenolic content and polyphenolic profiles of
Contents of phenolic compounds are expressed as as mg compound/g Dry plant weight. Values followed by the same letter did not share significant differences at 5% (Duncan test).
Alternatively, results reported by Parejo et
The polyphenolic profile of rosemary has been widely described in the scientific literature [4, 5, 16, 17, 29, 31, 33, 39, 44, 45, 46, 47, 48, 49]. The polyphenolic profile of these plants is characterized by the presence of carnosic acid, carnosol, rosmarinic acid and hesperidin, as major components. Based on the retention times of calibration standards, methanolic extracts of rosemary showed a phenolic profile composed of 18 identified phenolic compounds (Table 2). The polyphenolic profile of rosemary are composed of four phenolic acids (salvianic acid, caffeic acid, rosmarinic acid, and salvianic acid A), five phenolic diterpenes (Rosmadial, 7-CH3-Rosmanol, carnosol, carnosic acid and12-CH3-carnosic acid), and nine flavonoids (Luteolin −7-O-Rutinoxide, luteolin-7-Glucoronide, hesperidin, luteolin, apigenin, cirsimaritin, genkwanin, and salvigenin). Among the mentioned phenolic compounds, carnosic acid and carnosol were the major diterpenic components quantified in rosemary extracts, considering both provenances, (ranging from 46.3 to 76.4 mg/g and 22.4 to 43.5 mg/g respectively) followed by rosmarinic acid and hesperidin. Much lower contents were detected for luteolin, apigenin, cirsimaritin and genkwanin.
In the present study locations belonging to two different bioclimatic regions were prospected, showing, as a general pattern, that the differences in polyphenolic content should be attributed more to the genetic inheritance of the plants, than to the area of prospection. Contrary to this, studies accomplished by Yeddes et
Several phenolic compounds of rosemary determined in this study were similar in content and concentration to those in previous reports [1, 24, 51]. These phenolic compounds in rosemary extracts are very potent antioxidants and are utilized in many food products. The identified compounds were previously reported in
Rosemary (
This work was supported by the Tunisian Ministry of Higher Education, Scientific Research and Technology. The author thanks the Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA). La Alberca (Murcia), 30150, Spain, under which part of this work was carried out.
The authors declare no conflict of interest.
IntechOpen publishes different types of publications
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