Dr. Pletser’s experience includes 30 years of working with the European Space Agency as a Senior Physicist/Engineer and coordinating their parabolic flight campaigns, and he is the Guinness World Record holder for the most number of aircraft flown (12) in parabolas, personally logging more than 7,300 parabolas.
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Seeing the 5,000th book published makes us at the same time proud, happy, humble, and grateful. This is a great opportunity to stop and celebrate what we have done so far, but is also an opportunity to engage even more, grow, and succeed. It wouldn't be possible to get here without the synergy of team members’ hard work and authors and editors who devote time and their expertise into Open Access book publishing with us.
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Over these years, we have gone from pioneering the scientific Open Access book publishing field to being the world’s largest Open Access book publisher. Nonetheless, our vision has remained the same: to meet the challenges of making relevant knowledge available to the worldwide community under the Open Access model.
\\n\\n
We are excited about the present, and we look forward to sharing many more successes in the future.
\\n\\n
Thank you all for being part of the journey. 5,000 times thank you!
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Now with 5,000 titles available Open Access, which one will you read next?
Preparation of Space Experiments edited by international leading expert Dr. Vladimir Pletser, Director of Space Training Operations at Blue Abyss is the 5,000th Open Access book published by IntechOpen and our milestone publication!
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"This book presents some of the current trends in space microgravity research. The eleven chapters introduce various facets of space research in physical sciences, human physiology and technology developed using the microgravity environment not only to improve our fundamental understanding in these domains but also to adapt this new knowledge for application on earth." says the editor. Listen what else Dr. Pletser has to say...
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Dr. Pletser’s experience includes 30 years of working with the European Space Agency as a Senior Physicist/Engineer and coordinating their parabolic flight campaigns, and he is the Guinness World Record holder for the most number of aircraft flown (12) in parabolas, personally logging more than 7,300 parabolas.
\n\n
Seeing the 5,000th book published makes us at the same time proud, happy, humble, and grateful. This is a great opportunity to stop and celebrate what we have done so far, but is also an opportunity to engage even more, grow, and succeed. It wouldn't be possible to get here without the synergy of team members’ hard work and authors and editors who devote time and their expertise into Open Access book publishing with us.
\n\n
Over these years, we have gone from pioneering the scientific Open Access book publishing field to being the world’s largest Open Access book publisher. Nonetheless, our vision has remained the same: to meet the challenges of making relevant knowledge available to the worldwide community under the Open Access model.
\n\n
We are excited about the present, and we look forward to sharing many more successes in the future.
\n\n
Thank you all for being part of the journey. 5,000 times thank you!
\n\n
Now with 5,000 titles available Open Access, which one will you read next?
\n'}],latestNews:[{slug:"webinar-introduction-to-open-science-wednesday-18-may-1-pm-cest-20220518",title:"Webinar: Introduction to Open Science | Wednesday 18 May, 1 PM CEST"},{slug:"step-in-the-right-direction-intechopen-launches-a-portfolio-of-open-science-journals-20220414",title:"Step in the Right Direction: IntechOpen Launches a Portfolio of Open Science Journals"},{slug:"let-s-meet-at-london-book-fair-5-7-april-2022-olympia-london-20220321",title:"Let’s meet at London Book Fair, 5-7 April 2022, Olympia London"},{slug:"50-books-published-as-part-of-intechopen-and-knowledge-unlatched-ku-collaboration-20220316",title:"50 Books published as part of IntechOpen and Knowledge Unlatched (KU) Collaboration"},{slug:"intechopen-joins-the-united-nations-sustainable-development-goals-publishers-compact-20221702",title:"IntechOpen joins the United Nations Sustainable Development Goals Publishers Compact"},{slug:"intechopen-signs-exclusive-representation-agreement-with-lsr-libros-servicios-y-representaciones-s-a-de-c-v-20211123",title:"IntechOpen Signs Exclusive Representation Agreement with LSR Libros Servicios y Representaciones S.A. de C.V"},{slug:"intechopen-expands-partnership-with-research4life-20211110",title:"IntechOpen Expands Partnership with Research4Life"},{slug:"introducing-intechopen-book-series-a-new-publishing-format-for-oa-books-20210915",title:"Introducing IntechOpen Book Series - A New Publishing Format for OA Books"}]},book:{item:{type:"book",id:"9686",leadTitle:null,fullTitle:"Brassica Breeding and Biotechnology",title:"Brassica Breeding and Biotechnology",subtitle:null,reviewType:"peer-reviewed",abstract:"The family Brassicaceae constitutes one of the world’s most economically important plant groups. These plants are important sources of vegetable oil, vegetables, and condiments. Most of these crops belong to the genus Brassica, which includes common crops such as oilseeds (oilseed rape, mustard) and vegetables (broccoli, cauliflower, brussels sprouts, cabbage, turnip, Chinese cabbage, etc.). Brassica species play an essential role in horticulture and agriculture as well as contribute to the health of populations around the world. The current global climatic model predicts a significant decrease in growth, yield, and productivity of Brassica due to various biotic and abiotic stress factors. Thus, high-yielding, climate-resilient, and disease-resistant Brassica varieties are required to maintain as well as increase future agricultural production. The development of improved cultivars of these crops may become exhausted and improvement could become stagnant when plant breeding is merely based on a single breeding approach. Therefore, the goal of a breeding program should be to develop genetically superior Brassica cultivars suitable for a wide range of environments. This book examines the introgression of insect and disease resistance and other desirable traits into Brassica crops using inter-and/or intra-specific hybridization as well as biotechnological and molecular techniques, which could be useful for improving Brassica crops to ensure food security.",isbn:"978-1-83968-697-9",printIsbn:"978-1-83968-696-2",pdfIsbn:"978-1-83968-698-6",doi:"10.5772/intechopen.87490",price:119,priceEur:129,priceUsd:155,slug:"brassica-breeding-and-biotechnology",numberOfPages:174,isOpenForSubmission:!1,isInWos:1,isInBkci:!1,hash:"1afe175ea39b01d4e6e0c9d6427486a6",bookSignature:"A. K. M. Aminul Islam, Mohammad Anwar Hossain and A. K. M. Mominul Islam",publishedDate:"July 7th 2021",coverURL:"https://cdn.intechopen.com/books/images_new/9686.jpg",numberOfDownloads:3108,numberOfWosCitations:3,numberOfCrossrefCitations:9,numberOfCrossrefCitationsByBook:0,numberOfDimensionsCitations:12,numberOfDimensionsCitationsByBook:0,hasAltmetrics:0,numberOfTotalCitations:24,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"September 4th 2020",dateEndSecondStepPublish:"October 2nd 2020",dateEndThirdStepPublish:"December 1st 2020",dateEndFourthStepPublish:"February 19th 2021",dateEndFifthStepPublish:"April 20th 2021",currentStepOfPublishingProcess:5,indexedIn:"1,2,3,4,5,6",editedByType:"Edited by",kuFlag:!1,featuredMarkup:null,editors:[{id:"191072",title:"Prof.",name:"A. K. M. Aminul",middleName:null,surname:"Islam",slug:"a.-k.-m.-aminul-islam",fullName:"A. K. M. Aminul Islam",profilePictureURL:"https://mts.intechopen.com/storage/users/191072/images/system/191072.png",biography:"Prof. Dr. A. K. M. Aminul Islam is Professor of Genetics and Plant Breeding at Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur, Bangladesh, where he is also a director of research. He received bachelor’s and master’s degrees from Bangladesh Agricultural University (BAU), Mymensingh, Bangladesh, and a Ph.D. in Chemical and Process Engineering from Universiti Kebangsaan Malaysia. Dr. Islam is the author of 120 articles published in nationally and internationally reputed journals, twenty book chapters, and four books. He is an editorial board member and referee for several national and international journals. He is also the general secretary of the Plant Breeding and Genetics Society of Bangladesh, the seminar and research secretary of JICA Alumni Association of Bangladesh, and a lifetime member of several professional societies. Dr. Islam developed and released nineteen varieties of different crops for commercial cultivation by farmers. He supervised twenty-two MS and two Ph.D. students as major professor and forty MS and two Ph.D. students as a committee member. His major areas of research are the development of hybrid vegetables, canola-grade Brassica napus using a CMS system, and salinity-tolerant rice, as well as renewable energy research with Jatropha curcas.",institutionString:"Bangabandhu Sheikh Mujibur Rahman Agricultural University",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"4",totalChapterViews:"0",totalEditedBooks:"1",institution:{name:"Bangabandhu Sheikh Mujibur Rahman Agricultural University",institutionURL:null,country:{name:"Bangladesh"}}}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,coeditorOne:{id:"321236",title:"Prof.",name:"Mohammad Anwar",middleName:null,surname:"Hossain",slug:"mohammad-anwar-hossain",fullName:"Mohammad Anwar Hossain",profilePictureURL:"https://mts.intechopen.com/storage/users/321236/images/system/321236.png",biography:"Dr. Mohammad Anwar Hossain is a professor in the Department of Genetics and Plant Breeding, Bangladesh Agricultural University (BAU), Mymensingh, Bangladesh. He received his BSc in Agriculture and MS in Genetics and Plant Breeding from BAU. He also received an MSc in Agriculture from Kagawa University, Japan, in 2008, and a Ph.D. in Abiotic Stress Physiology and Molecular Biology from Ehime University, Japan, in 2011 through a Monbukagakusho scholarship. As a JSPS postdoctoral researcher in 2015–2017, he worked on isolating low-phosphorus, stress-tolerant genes from rice at the University of Tokyo, Japan. His current research program focuses on understanding physiological, biochemical, and molecular mechanisms underlying abiotic stress tolerance in crop plants and the generation of stress-tolerant and nutrient-efficient plants through breeding and biotechnology. He has more than sixty peer-reviewed publications to his credit and has edited twelve books, including this one.",institutionString:null,position:null,outsideEditionCount:null,totalCites:0,totalAuthoredChapters:"1",totalChapterViews:"0",totalEditedBooks:"0",institution:null},coeditorTwo:{id:"234696",title:"Prof.",name:"A. K. M. Mominul",middleName:null,surname:"Islam",slug:"a.-k.-m.-mominul-islam",fullName:"A. K. M. Mominul Islam",profilePictureURL:"https://mts.intechopen.com/storage/users/234696/images/system/234696.png",biography:"Dr. A. K. M. Mominul Islam is a professor in the Department of Agronomy, Bangladesh Agricultural University (BAU), Mymensingh, Bangladesh. He received both bachelor’s and master’s degrees from BAU. Dr. Islam obtained a second master’s degree in Physical Land Resources from Ghent University, Belgium, and a Ph.D. in Plant Allelopathy from United Graduate School of Agricultural Science, Ehime University, Japan, with his dissertation “Allelopathy of five Lamiaceae medicinal plant species.” Dr. Islam also completed his post-doctoral research at the Department of Horticulture and Landscape Architecture, Purdue University, Indiana, USA. Dr. Islam is the author of seventy-four articles published in nationally and internationally reputed journals, six book chapters, and three books. He is an editorial board and referee for several national and international journals. To date, he has supervised twenty-one MS students. Dr. Islam is currently supervising the research of two MS and six Ph.D. students in the areas of field crop production and management.",institutionString:"Bangladesh Agricultural University",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"2",totalChapterViews:"0",totalEditedBooks:"0",institution:{name:"Bangladesh Agricultural University",institutionURL:null,country:{name:"Bangladesh"}}},coeditorThree:null,coeditorFour:null,coeditorFive:null,topics:[{id:"341",title:"Olericulture",slug:"olericulture"}],chapters:[{id:"74816",title:"Advances in Breeding in Vegetable Brassica rapa Crops",doi:"10.5772/intechopen.95769",slug:"advances-in-breeding-in-vegetable-em-brassica-rapa-em-crops",totalDownloads:328,totalCrossrefCites:2,totalDimensionsCites:2,hasAltmetrics:0,abstract:"Brassica rapa includes oil and vegetable crops having a variety of forms, such as oilseeds, leafy vegetables and turnips. Leafy types, which are called turnip greens and turnip tops, are popular crops in NW Spain, and they represent an important part of the diet. However, their cultivation is limited in southern areas or in the Mediterranean basin, probably due to a lack of adaptation. Still, they could occupy a prominent place in the Mediterranean diet, which is based on a high consumption of fruits and vegetables. In this review, we summarize the studies on the agronomical and nutritional value of these crops when grown under Mediterranean climate conditions. Data reported here might be useful for a deeper understanding of these crops for both nutritional quality and bioaccessibility, and for selecting varieties adapted to the two abovementioned Mediterranean conditions, as well as for organic farming systems, thus contributing to the diversification of traditional Brassica vegetable production systems.",signatures:"María Elena Cartea, Fernando Cámara-Martos, Sara Obregón, Francisco Rubén Badenes-Pérez and Antonio De Haro",downloadPdfUrl:"/chapter/pdf-download/74816",previewPdfUrl:"/chapter/pdf-preview/74816",authors:[{id:"142197",title:"Prof.",name:"Fernando",surname:"Cámara-Martos",slug:"fernando-camara-martos",fullName:"Fernando Cámara-Martos"},{id:"229534",title:"Dr.",name:"Antonio",surname:"De Haro-Bailón",slug:"antonio-de-haro-bailon",fullName:"Antonio De Haro-Bailón"},{id:"334691",title:"Dr.",name:"María Elena",surname:"Cartea",slug:"maria-elena-cartea",fullName:"María Elena Cartea"},{id:"335952",title:"Dr.",name:"Sara",surname:"Obregon-Cano",slug:"sara-obregon-cano",fullName:"Sara Obregon-Cano"},{id:"342339",title:"Dr.",name:"Francisco",surname:"Badenes-Pérez",slug:"francisco-badenes-perez",fullName:"Francisco Badenes-Pérez"}],corrections:null},{id:"75542",title:"Rapeseed-Mustard Breeding in India: Scenario, Achievements and Research Needs",doi:"10.5772/intechopen.96319",slug:"rapeseed-mustard-breeding-in-india-scenario-achievements-and-research-needs",totalDownloads:651,totalCrossrefCites:3,totalDimensionsCites:4,hasAltmetrics:0,abstract:"Brassica spp., commonly known as rapeseed-mustard, plays a significant role in the Indian economy by providing edible oils, vegetables, condiments and animal feed. Globally, India holds second and third position in rapeseed-mustard area under cultivation and production, respectively. However, anthropogenically accelerated climate change thwarts yield potential of rapeseed-mustard by employing abiotic (drought, flood, temperature variation and salinity) and biotic (disease and insects) stresses. Various approaches such as molecular breeding, pre-breeding, −omics and biotechnological interventions have been used to develop varieties for improved yield and oil quality, climate resilient and resistance or tolerance to abiotic and biotic stresses. In this context, this chapter highlighted the different cytoplasmic male sterility (CMS) sources and their potential use for hybrid development. At the end, this chapter also enlisted salient achievement by the government and non-government institutes and briefly described the future perspective for improvement of rapeseed-mustard in India.",signatures:"Subhash Chand, Om Prakash Patidar, Rajat Chaudhary, Ranjit Saroj, Kailash Chandra, Vijay Kamal Meena, Omkar M. Limbalkar, Manoj Kumar Patel, Priya P. Pardeshi and Prashant Vasisth",downloadPdfUrl:"/chapter/pdf-download/75542",previewPdfUrl:"/chapter/pdf-preview/75542",authors:[{id:"249796",title:"Dr.",name:"Kailash",surname:"Chandra",slug:"kailash-chandra",fullName:"Kailash Chandra"},{id:"326742",title:"Dr.",name:"Subhash",surname:"Chand",slug:"subhash-chand",fullName:"Subhash Chand"},{id:"348860",title:"Dr.",name:"Om Prakash",surname:"Patidar",slug:"om-prakash-patidar",fullName:"Om Prakash Patidar"},{id:"348861",title:"Dr.",name:"Rajat",surname:"Chaudhary",slug:"rajat-chaudhary",fullName:"Rajat Chaudhary"},{id:"348862",title:"Dr.",name:"Ranjit",surname:"Saroj",slug:"ranjit-saroj",fullName:"Ranjit Saroj"},{id:"348863",title:"Dr.",name:"Vijay Kamal",surname:"Meena",slug:"vijay-kamal-meena",fullName:"Vijay Kamal Meena"},{id:"348865",title:"Dr.",name:"Omkar M.",surname:"Limbalkar",slug:"omkar-m.-limbalkar",fullName:"Omkar M. Limbalkar"},{id:"348867",title:"Dr.",name:"Manoj Kumar",surname:"Patel",slug:"manoj-kumar-patel",fullName:"Manoj Kumar Patel"},{id:"348868",title:"Dr.",name:"Priya P.",surname:"Pardeshi",slug:"priya-p.-pardeshi",fullName:"Priya P. Pardeshi"},{id:"348869",title:"Dr.",name:"Prashant",surname:"Vasisth",slug:"prashant-vasisth",fullName:"Prashant Vasisth"}],corrections:null},{id:"75023",title:"Innovative Strategies to Develop Abiotic and Biotic Stress Tolerance in Mustard (Brassicaceae)",doi:"10.5772/intechopen.95973",slug:"innovative-strategies-to-develop-abiotic-and-biotic-stress-tolerance-in-mustard-brassicaceae-",totalDownloads:323,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"Mustard crop is the third important source of vegetable oil randomly below soybean L. and palm, all over the world. Brassica crop is extremely susceptible to some biotic and abiotic stresses and they significantly influence the quality and quantity of the crop. In the past generally breeding techniques are used to develop resistance in mustard to avoid diseases though various pathogens are soon able to overcome that resistance by modifying their metabolic cycles. To bear the challenge there is an urgent need to develop abiotic as well as biotic stress tolerant plants using advanced techniques by understanding metabolic and biochemical pathways of plants and pathogens. Several techniques such selection of stress tolerance microbes, metabolite, enzymes, and genes are very important to avoid stresses. Whereas several techniques such as deployment of molecular markers for breeding, identification of Quantitative trait loci (QTL), in vitro tissue culture etc. can be more useful to improve biotic and abiotic stress tolerance in mustard. To develop healthy and high yield varieties, the mix of these techniques is needs to be implemented.",signatures:"Bahaderjeet Singh, Amanpreet Singh Sran and Gagandeep Singh Sohi",downloadPdfUrl:"/chapter/pdf-download/75023",previewPdfUrl:"/chapter/pdf-preview/75023",authors:[{id:"331114",title:"Assistant Prof.",name:"Bahaderjeet",surname:"Singh",slug:"bahaderjeet-singh",fullName:"Bahaderjeet Singh"}],corrections:null},{id:"75263",title:"Embryo Culture and Embryo Rescue in Brassica",doi:"10.5772/intechopen.96058",slug:"embryo-culture-and-embryo-rescue-in-em-brassica-em-",totalDownloads:323,totalCrossrefCites:1,totalDimensionsCites:1,hasAltmetrics:0,abstract:"Somatic embryogenesis is the best demonstration of totipotency in higher plants in which somatic cell produce whole plant like zygotic embryo. It is also demonstrated that immature, weak, hybrid or sometimes inviable embryos can be saved through in vitro culture to prevents its degradation. It may help to cross the reproductive barriers when interspecific hybrids developed. Brasssica is an economically valuable oil yielding and vegetable crop and India is the largest producer of oil seed rape in the world. Various factors affect the embryo rescue in Brassica like growth stage of the embryos, types and composition of the rescue medium etc. The embryo regeneration potential can improve through the modification of culture conditions in both zygotic as well as somatic embryo. Except the embryo culture other parts like ovule, ovary culture can also be done to developed interspecific hybrids. This chapter is focused on the embryo rescue techniques in the genus Brassica and summarizes possible ways of improving the technique used.",signatures:"Mohammad Akmal",downloadPdfUrl:"/chapter/pdf-download/75263",previewPdfUrl:"/chapter/pdf-preview/75263",authors:[{id:"181036",title:"Dr.",name:"Mohammad",surname:"Akmal",slug:"mohammad-akmal",fullName:"Mohammad Akmal"}],corrections:null},{id:"74043",title:"Breeding Mustard (Brassica juncea) for Salt Tolerance: Problems and Prospects",doi:"10.5772/intechopen.94551",slug:"breeding-mustard-em-brassica-juncea-em-for-salt-tolerance-problems-and-prospects",totalDownloads:262,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"Salt stress is currently one of the most critical factors, reducing agricultural production. Indian mustard (Brassica juncea) is a major oilseed crop in these areas. However, salt affects as much as 50–90% worldwide yield reduction. Salt tolerance is a very complex factor controlled by a number of independent and/or interdependent mechanisms and genetic modification that lead to many changes in physiology and biochemistry at the cellular level. The classical methods of plant breeding for salt tolerance involves the widespread use of inter and intraspecific variations in the available germplasm which is essential for any crop development program. This large germplasm is then tested under various salt levels in microplots, which is a quick, reliable, reproducible and inexpensive method of salt tolerance. Genotypes that have shown better indications of stress tolerance without significant yield reduction are considered to be tolerant and are also used as potential donor in the breeding programs. In this way, ICAR-Central Soil Salinity Research Institute (ICAR-CSSRI), Karnal developed and produced five varieties of Indian mustard that tolerate high salt namely, CS 52, CS 54, CS 56, CS 58 and CS 60 in the country, and many other high-quality pipeline lines exploration and development. These salt-tolerant species work better under conditions of salt stress due to various manipulations (physiology, genes and molecular level) to fight salt stress has led to detrimental effects. Recent molecular tools to add classical breeding systems to improve saline-tolerant mustard varieties in a short span of time, including the Marker Assisted Selection (MAS) and backcrossing, that have helped using simple sequence repeats (SSR) and single nucleotide polymorphisms (SNP) markers to identify quantitative trait loci (QTLs) that control the polygenic traits like tolerance of salt and seed yield.",signatures:"Jogendra Singh, Parbodh Chander Sharma and Vijayata Singh",downloadPdfUrl:"/chapter/pdf-download/74043",previewPdfUrl:"/chapter/pdf-preview/74043",authors:[{id:"245615",title:"Dr.",name:"Jogendra",surname:"Singh",slug:"jogendra-singh",fullName:"Jogendra Singh"}],corrections:null},{id:"75554",title:"Salinity Tolerance in Canola: Insights from Proteomic Studies",doi:"10.5772/intechopen.96649",slug:"salinity-tolerance-in-canola-insights-from-proteomic-studies",totalDownloads:316,totalCrossrefCites:2,totalDimensionsCites:3,hasAltmetrics:0,abstract:"Salinity considerably lowers crop yield worldwide. Production of salt stress-tolerant species will be essential to maintain the food supply in the coming decades. Brassicas, including various members of the family Brassicaceae, are very necessary sources of human food. Importantly, the key crop species that are members of the Brassicaceae family are genetically diverse and therefore their response reaction and adaptation to salinity varies greatly. Canola (Brassica napus L.) is commonly grown for edible oils and other uses such as biodiesel fuel production. Although most types of canola are identified as salt-resistant, plant yield and development are reduced significantly by rising salinity levels. In saline situations, the plant’s genome supports a range of physiological changes in some plant characteristics. Since the function of genes cannot indicate the exact condition of cells, proteomic approaches are emerged as methods to investigate the plant’s responses to stresses in the molecular levels. Exploring the proteome complements research at the genome and transcriptome level and helps elucidate the mechanism of salt tolerance in plants. Proteins are reliable indicators of salinity responses, as they are directly involved in forming the new phenotype providing adaptation to salinity. In this chapter, we review the response of the rapeseed proteome to salinity stress.",signatures:"Ali Bandehagh, Zahra Dehghanian, Robert Henry and Mohammad Anwar Hossain",downloadPdfUrl:"/chapter/pdf-download/75554",previewPdfUrl:"/chapter/pdf-preview/75554",authors:[{id:"321236",title:"Prof.",name:"Mohammad Anwar",surname:"Hossain",slug:"mohammad-anwar-hossain",fullName:"Mohammad Anwar Hossain"},{id:"333759",title:"Dr.",name:"Ali",surname:"Bandehagh",slug:"ali-bandehagh",fullName:"Ali Bandehagh"},{id:"333782",title:"BSc.",name:"Zahra",surname:"Dehghanian",slug:"zahra-dehghanian",fullName:"Zahra Dehghanian"},{id:"349347",title:"Dr.",name:"Robert",surname:"Henry",slug:"robert-henry",fullName:"Robert Henry"}],corrections:null},{id:"75610",title:"Epidemiology, Genetics and Resistance of Alternaria Blight in Oilseed Brassica",doi:"10.5772/intechopen.96454",slug:"epidemiology-genetics-and-resistance-of-em-alternaria-em-blight-in-oilseed-em-brassica-em-",totalDownloads:272,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"Alternaria blight is one of the most deadly diseases of oilseed Brassica. This recalcitrant disease causes up to 50% yield loss across the globe. The disease is mainly caused by Alternaria brassicae and Alternaria brassicicola. These pathogens lack sexual stages and survive as conidia or condiospores on the debris of previous crops and susceptible weeds. Developing resistant oilseed Brassica cultivars to this disease has become a prime concern for researchers over the years. In absence of resistant oilseed Brassica cultivar, identification and introgression of resistance related genes can be a potential source for Alternaria blight resistance. As resistance toward Alternaria blight is governed by polygenes, intercrossing between the tolerant genotypes and subsequent selection will be the most appropriate way to transfer the quantitative resistance. For that reason, future breeding goal should focus on screening of germplasms for selecting genotypes containing resistance genes and structural features that favors resistance, like thick epicuticular wax, biochemical components such as phenols, phytoalexins and lower soluble sugars, reducing sugars and soluble nitrogen. Selected genotypes should be brought under appropriate breeding programs for attaining Alternaria blight resistance.",signatures:"Subroto Das Jyoti, Naima Sultana, Lutful Hassan and Arif Hasan Khan Robin",downloadPdfUrl:"/chapter/pdf-download/75610",previewPdfUrl:"/chapter/pdf-preview/75610",authors:[{id:"322667",title:"Prof.",name:"Arif Hasan Khan",surname:"Robin",slug:"arif-hasan-khan-robin",fullName:"Arif Hasan Khan Robin"},{id:"329291",title:"Prof.",name:"Lutful",surname:"Hassan",slug:"lutful-hassan",fullName:"Lutful Hassan"},{id:"330722",title:"Mr.",name:"Subroto Das",surname:"Jyoti",slug:"subroto-das-jyoti",fullName:"Subroto Das Jyoti"},{id:"342026",title:"Ms.",name:"Naima",surname:"Sultana",slug:"naima-sultana",fullName:"Naima Sultana"}],corrections:null},{id:"75379",title:"Breeding for Disease Resistance in Brassica Vegetables Using DNA Marker Selection",doi:"10.5772/intechopen.96263",slug:"breeding-for-disease-resistance-in-brassica-vegetables-using-dna-marker-selection",totalDownloads:341,totalCrossrefCites:1,totalDimensionsCites:2,hasAltmetrics:0,abstract:"The Brassica genus comprises of agro-economically important vegetables. Disease causes great yield loss of Brassica vegetables worldwide. Different traditional methods such as crop rotation and chemical control have limited effect on different diseases of Brassica vegetables and cannot completely eradicate the pathogens by these methods. Development of disease resistant cultivars is one of the most effective, ecofriendly, and cheapest measure to control Brassica diseases. With the development of genomics, molecular biology techniques, and biological methods, it is possible to discover and introduce resistance (R) genes to efficiently control the plant diseases caused by pathogens. Some R genes of major diseases such as Fusarium wilt and clubroot in Brassica vegetables have been already identified. Therefore, we will focus to review the Fusarium wilt and clubroot resistance in Brassica vegetables and the methodologies for identification, mapping, and pyramiding of R genes/quantitative trait loci (QTLs) to develop disease resistant cultivars. These techniques will be helpful for sustainable crop production and to maintain global food security and contribute to ensure protection of food supply in the Asian country as well as throughout the world.",signatures:"Mst Arjina Akter, Hasan Mehraj, Takeru Itabashi, Tomoe Shindo, Masaaki Osaka, Ayasha Akter, Naomi Miyaji, Naoki Chiba, Junji Miyazaki and Ryo Fujimoto",downloadPdfUrl:"/chapter/pdf-download/75379",previewPdfUrl:"/chapter/pdf-preview/75379",authors:[{id:"233726",title:"Dr.",name:"Ryo",surname:"Fujimoto",slug:"ryo-fujimoto",fullName:"Ryo Fujimoto"},{id:"233727",title:"Ms.",name:"Ayasha",surname:"Akter",slug:"ayasha-akter",fullName:"Ayasha Akter"},{id:"345922",title:"Ms.",name:"Mst Arjina",surname:"Akter",slug:"mst-arjina-akter",fullName:"Mst Arjina Akter"},{id:"345923",title:"Dr.",name:"Hasan",surname:"Mehraj",slug:"hasan-mehraj",fullName:"Hasan Mehraj"},{id:"345924",title:"Mr.",name:"Takeru",surname:"Itabashi",slug:"takeru-itabashi",fullName:"Takeru Itabashi"},{id:"345925",title:"Ms.",name:"Tomoe",surname:"Shindo",slug:"tomoe-shindo",fullName:"Tomoe Shindo"},{id:"345926",title:"Mr.",name:"Naoki",surname:"Chiba",slug:"naoki-chiba",fullName:"Naoki Chiba"},{id:"345927",title:"Dr.",name:"Masaaki",surname:"Osaka",slug:"masaaki-osaka",fullName:"Masaaki Osaka"},{id:"345928",title:"Ms.",name:"Naomi",surname:"Miyaji",slug:"naomi-miyaji",fullName:"Naomi Miyaji"},{id:"345929",title:"Dr.",name:"Junji",surname:"Miyazaki",slug:"junji-miyazaki",fullName:"Junji Miyazaki"}],corrections:null},{id:"75968",title:"Brassica-Aphid Interaction: Modulated Challenges and Sustainable Approach for Management",doi:"10.5772/intechopen.96903",slug:"brassica-aphid-interaction-modulated-challenges-and-sustainable-approach-for-management",totalDownloads:294,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"Insect pests act as main barrier in enhancing yield potential of Brassica crops. Lipaphis erysimi is considered as one of the most destructive insect species in mustard production due to its voracious type feeding and multiplication. Therefore application of insecticide is inevitable for cultivation of cruciferous crops, although systemic insecticides has been found to be suitable for management of aphid, despite of high cost, residual effect and ecological ramification have necessitated the application of bio and botanical insecticides as novel approach and are recorded significant in research. Aphids having exclusively viviparous parthenogenesis type reproduction from January to March month with the completion of eight generations are helpful in quick mass multiplication. Natural enemies Coccinella spp., Syrphid larvae and bio-pesticide found effective in suppress aphid numbers. Manipulation in sowing dates of mustard crop provides good yield and less incidence of aphid which is proved through research. Lack of environmental resistant varieties has dispensed toward non feasibility of conventional breeding approaches for developing aphid-resistant Brassica. Although application of genetic engineering plan has resulted in moderate success in development of aphid resistance, so far commercialization of such genetically modified crops has not conceivable, intimate the necessity of further insights in to host plant and aphid communication to form effective approach against aphid resistance. Therefore in this chapter the components involved in Brassica aphid communication are highlighted and present statuses and problem in aphid management are discussed.",signatures:"S.A. Dwivedi, Lelika Nameirakpam and Ajay Tomer",downloadPdfUrl:"/chapter/pdf-download/75968",previewPdfUrl:"/chapter/pdf-preview/75968",authors:[{id:"330948",title:"Dr.",name:"S.A.",surname:"Dwivedi",slug:"s.a.-dwivedi",fullName:"S.A. 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\n
1. Introduction
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Oral health is part of general health and is essential to people’s well-being. Good oral health implies being free from chronic orofacial pain, oral and pharyngeal cancer, soft tissue changes in the mouth, congenital disabilities, and other issues affecting the craniofacial complex [1].
Active performance of the dentist in care and prevention actions
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Population characteristics: low sociocultural level, age, and gender
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Training of the oral health team on oral cancer
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HPV infection
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Implementation of new early detection strategies, according to the population served
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Prolonged use of dental prosthesis, especially in the absence of routine monitoring and evaluation by a professional
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Individual and collective educational actions on healthy living habits
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Lack of information about the disease, its risk factors and protection
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Routine screening for early detection of cancer, especially among the most vulnerable to oral cancer
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Lack of information to the population about health services and their flow when a suspected injury is diagnosed
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Integration of the oral health team with smoking control programs and other actions related to oral cancer control
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Lack of training of professionals working in care
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Systematic information to the population on reference sites for oral cancer diagnostic examination
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Lack of specific campaigns and information about oral cancer, its risk factors and protection
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Fight against lifestyle, environmental, and occupational factors that may be related to oral cancer
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Investigation of genetic factors associated with some specific types of cancer for the risk group
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Tracking of this pathology in at-risk populations by the health team
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Offering opportunities for evaluation of oral lesions (active search—through home visits or specific campaigns)
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\n
\n
Follow-up of suspected cases, creating a reference service if necessary and establishing partnerships between universities and other organizations for prevention, diagnosis, treatment, and recovery
\n
\n
\n
\n
Training of professionals working in the front line of health care
\n
\n\n
Table 1.
Summary of risk factors related to oral cancer and primary and secondary prevention methods.
\n
\n
\n
\n
\n
\n\n
\n
Method
\n
Indication
\n
Advantage
\n
Disadvantage
\n
\n\n\n
\n
Toluidine blue test
\n
Diagnosis and surgical approach to various mucosal tumors
\n
Painless, low-cost, easy application, fast result, and high sensitivity
\n
It can generate false-positive or false-negative results, being of low specificity
\n
\n
\n
Exfoliative cytology
\n
Initial assessment of incipient lesions and follow-up of areas that underwent previous surgical resection
\n
Painless, harmless, noninvasive, inexpensive
\n
It does not have the same efficacy as biopsy in the identification of the type of lesion; however it is very useful when biopsy is not possible. It can generate false-positive or false-negative results
\n
\n
\n
Fluorescence/autofluorescence
\n
Adjuvant method in oral clinical examination for detection of cellular disorders
\n
High sensitivity for cancer and dysplasia, ability to evaluate large areas of the oral mucosa at the moment of consultation, noninvasive, painless
\n
It can generate false-positive results
\n
\n
\n
Contact endoscopy
\n
A colposcope with optical magnification of up to 40 times is applied to help diagnose oral cavity lesions
\n
Painless, fast diagnosis
\n
Difficulties in relation to the device and the anatomical structures examined (lip and oral cavity) related to the contact of the lens surface, fine tremors, and slippage of the device
\n
\n
\n
In vivo microscopy
\n
Histopathological evaluation of suspected lesions at the moment of consultation
\n
High precision
\n
High cost
\n
\n
\n
Tumor biomarkers
\n
Diagnostic elucidation, tumor recurrence evaluation, or follow-up of treatment progress
\n
Early detection, noninvasive
\n
It can generate false-positive or false-negative results
\n
\n
\n
Oral inspection and palpation
\n
Identification of lesions, monitoring of oral health of the individual, screening of suspicious lesions for oral cancer
\n
Fast, painless, and low-cost
\n
It depends on the examiner’s skill and knowledge
\n
\n\n
Table 2.
Noninvasive methods for diagnosis of oral cancer.
\n
Oral cancer (OC) is considered a public health problem because of its high mortality and morbidity rates. This problem also affects most people with low sociocultural level and who are alcoholics and smokers. However, there are other associated risk factors: chewing tobacco, use of a dental prosthesis, infection with human papilloma virus (HPV) type 16, nutritional deficiency, age, gender, poor oral hygiene, excess body fat, and chewing betel nut, among others [2, 3].
\n
Data published by the International Agency for Research on Cancer (IARC) regarding cancer cases in general reported in 2012 about 14.1 million new cases, 8.2 million deaths within 5 years of diagnosis, and 32.6 million people living with cancer in the world. Of these, approximately 57% of new cases, 65% of deaths, and 48% of cases diagnosed in the last 5 years are in developing countries [4]. In 2018, new data were released, indicating an increase in new cases of cancer, with 18.1 million new cases and 9.6 million deaths [5]. The significant increase of this disease is clear, indicating the need for new plans for prevention and early diagnosis.
\n
Regarding oral cancer, the highest rates have been observed in populations of Melanesia, Central-South Asia, Eastern and Western Europe, Africa, and Central America. Oral cancer is the sixth most common in the world, and most cases occur in India and Southeast Asia, according to the estimates for 2012 [6]. A change was seen in 2018 when oral cancer ranked in the 11th position among the most common cancers in the world. This type of cancer is quite common in Brazil, which has the third highest incidence in the world, behind only India and former Czechoslovakia [5].
\n
Oral cancer is a condition that negatively interferes with the general and oral health of the individual. These oral problems cause pain and infection, leading to psychological and physical distress. It is important to note that such dental conditions express social exclusion. In general, they are associated with poor education, low income, unemployment, and difficulty in accessing care services [7]. Thus oral cancer patients represent a group of people that should receive differentiated attention because, besides cancer itself, they are highly susceptible to other ills [2]. When this disease affects individuals, they may have to face consequences such as facial mutilation. Also, they may render them unable to work, with severe damage to their quality of life. The disease may sometimes be lethal, mainly because of late diagnosis.
\n
This pathology causes essential changes in the daily lives of the affected people, interfering with their body image, body functioning, and psychological, social, and family structure. The disease mostly affects the population in their working phase of life, causes indirect damage to the country. Late diagnosis is directly associated with shorter survival. However, if diagnosed early, it has a good prognosis and an average 5-year survival rate of 77.3% in stages I and II, but of 32.2% in stages III and IV [2].
\n
Morbidity and mortality rates are high, with diagnosis in advanced phases in 65–85% of the cases, reducing the likelihood of cure [2, 8, 9, 10, 11]. For most of these patients, palliative care is the only option available to achieve a better quality of life and control symptoms.
\n
Protective factors against this condition include general and specific measures. The adoption of healthy lifestyle habits, including adequate nutrition, physical exercise, and self-care, is part of the prevailing standards. In turn, specific practices include oral health care, routine inspection of the oral cavity, periodic dental evaluation, and cessation of smoking and alcoholism, and recent studies have shown the consumption green tea as a protective measure [12, 13].
\n
Although oral cancer is easily detected, its diagnosis is late in most cases. It is possible to improve diagnosis through the use of health promotion and prevention measures and improved access to health services, to promote early diagnosis [7, 14, 15]. Diagnosis is followed by curative treatment, preventing mutilating and disabling sequelae.
\n
The relevance of this disease and its early diagnosis should be considered for the possibility of curative treatment and promotion of the quality of life of patients. It is essential to know and recommend methods that act in favor of the early diagnosis of this pathology. It may mean identifying early malignant and even premalignant lesions, leading to the cure of these patients and rehabilitation to their social routine. It is equally important to act on factors that influence to late diagnosis of this pathology, through the planning of actions.
\n
\n
\n
2. Factors related to late diagnosis of oral cancer
\n
The problem of delayed diagnosis of OC is known worldwide, and each country or region has different strategies to address it. These factors are described in several studies [16, 17], showing that this is a global problem. Factors related to late diagnosis of OC concern the social determinants, health literacy, and characteristics of the health system:
Profile of the affected people concerning lifestyle habits: most people who develop OC were smokers and alcoholics and are in situations associated with other unhealthy lifestyles, such as poor diet and physical inactivity [2].
Lack of self-care in oral health: the most vulnerable populations, which are those with a low socioeconomic level, frequently have poor self-care due to their living conditions, especially in terms of oral health, besides other health problems. This problem directly interferes with their quality of life and interaction with peers. They also present a low search for health care, leading to the worsening of health problems and, in this case, late diagnosis of oral cancer [18].
Delayed perception of the lesion: due to poor self-care, most do not identify the presence of initial lesions in the oral cavity. Thus, injuries are only perceived when they cause discomfort, pain, bleeding, or other symptoms, and at that point, in most cases, the disease is already in an advanced stage [18].
Lack of information about oral cancer and its protective and risk factors: many campaigns for the dissemination of information on disease have been promoted, but specifically on oral cancer is still incipient. People asked to appear to not know about oral cancer, suspected lesions or risk, and protective factors, even patients who are undergoing treatment for this type of cancer [14, 19].
Lack of health promotion and prevention activities aimed at oral cancer: a few specific actions to promote and prevent this type of cancer are carried out. These actions are usually linked to other campaigns such as those focused on vaccination, smoking, and oral health in general [14].
Lack of training in oral cancer among oral health professionals and deficits in addressing this content in the curricula of undergraduate courses: oral health professionals are not routinely updated and trained on this content. Still, the approach during undergraduate training is deficient, producing professionals with little experience to approach patients with suspected lesions [14, 16].
Delayed search for professional assistance when the patient perceives the lesion: people usually notice the presence of the injury but do not seek a professional for confirmation. Often they refer to fear of confirming the diagnosis of the disease. This delay in seeking the diagnosis causes the lesion to continue growing, leading to late diagnosis [14].
Difficulties in accessing dental treatment: many people are unable to get adequate dental care due to the difficulty in accessing health services. In general, it is due to their vulnerable conditions or even because they do not seek health services [14].
\n\n
Regarding oral cancer prevention and health promotion activities, it is essential to highlight the urgency of designing public policies for long-term health education actions. If education is not changed, concepts and habits will not change after short campaigns. That points out to the need for permanent education programs, since the best way to combat oral cancer is prevention, early diagnosis, and the attempt to eliminate risk factors. Health education through programs aimed at valuing periodic evaluations and the importance of examining the oral cavity are the significant weapons available to reduce the high incidence of oral cancer in the community [14].
\n
The biopsy is undoubtedly the gold standard for the diagnosis of OC. However, there are several questions related to this method used in screening. It is an aggressive procedure, not readily accepted by people, especially when the lesion is asymptomatic and, even more so if it is proposed in oral health campaigns. The biopsy is limited by morbidity, once the procedure provokes another injury that may cause pain, bleeding, or other symptoms. Still, due to the resources required and the possibility of underdiagnosis, this method demands trained professionals to perform the procedure, trained pathologist, and facilities for the necessary reading of the exam. These characteristics mean a long time to receive the diagnosis, and patients experience discomfort caused by all the process [20].
\n
Although the factors related to early detection of OC have different natures, it is noteworthy that, after all, the primary responsibility lies with the health system. For that, health service and program must organize its strategies according to the characteristics of the users.
\n
Studies have been conducted to support measures aimed at solving the problem of late diagnosis, and the various approaches used to solve this problem are related to the factors abovementioned. In this text, we will address in particular the issue of noninvasive methods.
\n
\n
\n
3. Prevention of oral cancer
\n
Considering factors that interfere with the pathology diagnosis in question, the actions should be directed to them, to improve the care to the population.
\n
Prevention and early diagnosis of oral cancer are critical. Equally important is the need for a differentiated look in this issue, given the characteristics of the affected population, the role of the dentist, the continuing training of oral health professionals, and the implementation of new strategies for early detection of this pathology [16, 21].
\n
Actions related to the prevention of oral cancer and early diagnosis are foreseen within concepts widely worked in public health, which are health promotion and disease prevention. Health prevention requires firstly action based on knowledge of the natural history of the disease to prevent its progress [22]. Primary prevention is defined as a set of interventions to minimize the risk of specific ailments, reducing incidence and prevalence rates in the population and focusing on keeping individuals free from diseases.
\n
Health promotion, on the other hand, is broader, as it refers to measures that act in the health disease process. Here, the intention is to modify the lifestyle and living conditions of the population, thus not working on a specific disease. Health promotion depends on the individuals, the community, and the sectors of society, health professionals, and oral health professionals [23].
\n
Preventive measures may have a collective or individual approach. The collective approach includes interventions focused on health promotion. It means educational actions, periodic examinations of the most vulnerable people to the development of oral cancer, integration of the oral health team into smoking control programs, and other actions related to control of oral cancer. Besides that is a systematic provision of information on reference sites to the population about the diagnostic examination of oral cancer. In turn, the individual approach includes early diagnosis, treatment, and rehabilitation [17, 18].
\n
Some primary intervention and prevention measures would be ideal for reducing cancer, such as combating lifestyle, environmental, and occupational factors and investigating the genetic factors associated with some specific types of cancer [14]. Population screening is indicated as an important preventive measure. This process can favor the diagnosis of suspicious lesions, which are to be referred for differential diagnosis, making it possible to implement early intervention and increase the chance of cure [14, 24]. Studies indicate the relevance of the screening of this pathology in risk populations, such as smokers and alcoholics. And it is even more relevant given the delay in diagnosis. Although evidence from the use of the visual examination of the mouth on mortality rates is weak by OC, some authors suggest proceeding to the screening on individuals who are exposed to risk factors. For these people, it may result in an increased positive predictive factor [7].
\n
The approach to OC should involve prevention and control measures, including routine screening for early detection; offer of opportunities for evaluation of oral lesions (active search—through home visits or specific campaigns); follow-up of suspected cases, creating a referral service if necessary; and establishing partnerships between universities and other organizations for prevention, diagnosis, treatment, and recovery [18].
\n
Actions with this objective can be organized as primary prevention. They include activities geared at disseminating information to the population, intending to change unhealthy lifestyle habits to healthy ones and to reduce the prevalence and incidence rates. At this level, the emphasis is placed on drinking, smoking, diet, and exposure to sun and human papillomavirus (HPV) infection.
\n
Secondary prevention, in turn, occurs through the identification of precancerous lesions. For correct identification, it is essential to train health-care professionals with an emphasis on assessing potentially malignant cell lesions/disorders (PMD) (DPM) [17].
\n
Below in Table 1 is a summary of the risk factors and prevention methods.
\n
\n
\n
4. Noninvasive methods for diagnosis of OC
\n
Easy-to-handle, noninvasive diagnostic methods are useful for identifying precancerous lesions. The following noninvasive methods are cited: toluidine blue testing, exfoliative cytology, autofluorescence, contact endoscopy, and in vivo microscopy. However, there is no scientific evidence that these methods are more effective than oral inspection and palpation. Thus, more extensive studies are needed to justify the widespread use of these methods in the population. However, studies have shown that these methods can be useful if used in people with risk factors and non-healing lesions, favoring a faster diagnosis [20, 25, 26].
\n
The following noninvasive diagnostic methods should be used according to the possibilities and conditions of the context.
\n
\n
4.1 Toluidine blue test
\n
Toluidine blue is a basic thiazine metachromatic dye that selectively marks acidic groups of tissue components (carboxylic radicals, sulfates, and phosphates), showing an affinity for nuclear DNA and cytoplasmic RNA, which fix the dye, becoming richly stained. The intensity of toluidine blue staining depends on the degree of involvement of the epithelial surface. In benign lesions, there is a faint coloration; in dysplasia and epithelial lesions and carcinomas, the coloration is more intense.
\n
The application maneuver consists of drying and isolating the region to be examined from salivary contamination by grasping the site with the fingers and using gauze. Employing a flexible cotton swab, 1% acetic acid (acid solution) is applied to clean the lesion surface, remove the glycoprotein barrier of cells, and promote slight dehydration of the mucosa. After 1 minute, the AT dye is applied with the other side of the cotton swab, and after 1 minute, the excess is cleaned with 1% acetic acid again and washed with plenty of water.
\n
The result is intended to highlight intensely stained areas compatible with areas of tissue degeneration. Indications: detection of epithelial dysplasias, in situ or early invasive carcinomas, delimitation of neoplastic epithelium margins, assessment of tumor recurrence after surgical or radiotherapy treatment, delimitation of areas of cancer action, screening of oral lesions in population groups exposed to risk factors for oral cancer, and in intraoperative actions for marginal control of carcinomas.
\n
It has the advantages of being painless, low-cost, and easy to apply, giving fast results, and having high sensitivity. As for disadvantages, it may generate false-positive or false-negative results and be of low and little specificity [10, 27].
\n
\n
\n
4.2 Exfoliative cytology
\n
It can be defined as the morphological and morphometric study of desquamated cells of the mucosa, mainly suprabasal cells, through optical microscopy. It consists in the examination of cells from various parts of the body to determine the cause or nature of the disease that affects them.
\n
There are reports of numerous methods for collection of these cells in the literature. Conventional exfoliative cytology and liquid-based exfoliative cytology are two of the most disseminated among them.
\n
Collecting the material in exfoliative cytology involves scraping the surface of the lesion with a spatula or brush, which is then smeared over the glass slide, and the material is fixed to the slide using 95% alcohol or 1:1 alcohol/ether solution.
\n
Exfoliation cytology in liquid media has been developed in recent years as a method that could replace the conventional exfoliative cytology proposed by Papanicolaou. Collection by this method is done using a brushing device with soft bristles arranged in a conical shape, which is then dipped in a methanol-based preservative liquid contained in a hermetically sealed tube. Such liquid has the function of preserving the cellular structure, the proteins, and principally the genetic material. The liquid undergoes a centrifugation or homogenization process, which helps to shrink some artifacts, and it is then filtered. The residual material in the filters is put in blades by contact imprinting. Debris, red blood cells, and mucus pass through the filter pores, which retain the epithelial cells to be analyzed.
\n
It has the advantages of being painless, harmless, noninvasive, and low-cost. As for disadvantages, it does not have the same efficacy as biopsy concerning identifying the type of lesion, but it is beneficial when the biopsy is not possible [20, 28, 29].
\n
\n
\n
4.3 Fluorescence/autofluorescence
\n
Optical fluorescence can be used as an aid to oral clinical examination. It allows, by autofluorescence, the detection of numerous changes in the oral cavity that could go unnoticed by the dentist or even be difficult to perceive with the visual method alone.
\n
The oral fluorescence system allows the observation of changes in dental hard tissues such as stains, dental plaque and calculus, incipient lesions, and marginal infiltrations and facilitates the differentiation between restorative materials such as composite resin and ceramic.
\n
In soft tissues, it is possible to detect potentially malignant lesions and tumoral lesions. Therefore, the optical fluorescence system allows the simple, noninvasive, and real-time diagnosis and identification of structures and alterations in the oral cavity, revealing lesions that would not be easily detected with conventional illumination.
\n
As advantages, this method is highly sensitive to cancer and dysplasia, allows the evaluation of large areas of the oral mucosa during a consultation, and is noninvasive and painless. However, it has the disadvantage of false-positive results [20].
\n
\n
\n
4.4 Contact endoscopy
\n
It is also known as contact microstomatoscopy. It consists of the contact of the endoscope lens with the mucosa, the vermilion, and or the lesion.
\n
It has the advantages of being painless and providing a fast diagnosis. However, a study by showed that the difficulties encountered about the device and the anatomical structures examined (lip and oral cavity) were related to the contact of the lens surface, fine tremors, and the sliding of the device; these difficulties varied according to topography. The quality depended on the site of the lesion, the extent of the ulceration, the volume of crusts, prior cleaning of the site, patient collaboration, the presence of more or less saliva, the mobility of the examined structure, and the support for the device [28, 30].
\n
\n
\n
4.5 In vivo microscopy
\n
High-resolution microendoscopy, optical coherence tomography, confocal reflectance microscopy, and multiphoton imaging are considered in this classification. These methods allow practitioners to see many of the same microscopic features used for histopathological evaluation at the consultation.
\n
Each technology measures different optical properties of the tissue and offers various features in parameters such as image depth, resolution, visual field, and acquisition time. Their development is at an early stage. We cite Raman spectroscopy as a promising technique for cancer diagnosis. This device is an analytical noninvasive technique that provides information about the molecular structure of the investigated sample, considering that the molecular structures of proteins and lipids differ between normal and neoplastic tissues.
\n
The advantage of these technologies is their accuracy, but the high cost of acquisition is a significant disadvantage [20, 31].
\n
\n
\n
4.6 Tumor biomarkers
\n
Tumor biomarkers are substances found in blood, urine, or other body fluids and tissues that may be in increased amounts when a particular type of cancer is present. These biomarkers are used for diagnostic elucidation through serology and histological methods. They are cellular, structural, and biochemical components that can be quantitatively measured by biochemical, immunological, and molecular methods in body fluids and tissues associated with neoplasms and possibly the organs where cancer originates.
\n
At present, no marker is used for cancer detection in the general population, only for people who are in the risk group for certain types of cancer. In this case, biomarkers can help to diagnose the disease in early stages.
\n
Research on the diagnosis of saliva using nanotechnology and molecular technologies to detect oral squamous cell carcinoma (OSCC) is currently being expanded. Collecting saliva for this assessment is a secure, noninvasive method, which is considered advantageous.
\n
Diagnosing saliva using nanotechnology and molecular technologies to detect OSCC has become an attractive field of study. New cancer-related proteins have been reported, as well as potent biomarkers for early diagnosis, further facilitating the application of quantification in proteomics for carcinogenesis research. Identifying transcripts and pathways that change at early stages of carcinogenesis provides potentially useful information for early diagnosis and prevention strategies.
\n
At the beginning of the research on this method, the hope was that all cancers could be detected at an early stage, preventing the death of millions of people. But only a few markers can detect cancer at an early stage. The disadvantages of this method are most people have a small amount of these markers that prevents detection, the levels of these markers tend to increase when the disease progresses, some cancer patients may never have high levels of markers, and even in the presence of elevated levels, they do not always indicate cancer, as they may be related to other disorders [6, 32, 33, 34, 35, 36].
\n
\n
\n
4.7 Oral inspection and palpation
\n
The main areas examined for oral cancer are the face, neck, lips, nostrils, and oral cavity. Before the screening, the patient should remove all removable dental appliances and devices to leave the entire area exposed. The patient must be seated or lying down, and the dentist must look for signs of asymmetry, edema, swelling, staining, ulceration, or other abnormalities.
\n
To examine the inside of the mouth, the practitioner will use good lighting and a mirror to see clearly; he will also use a tongue depressor to immobilize the organ and look at the back of the mouth. After or during the visual examination, the dentist will palpate the head, face, around the jaw, under the chin, and the oral cavity. The aim is to detect unusual lumps or masses. Another sign of a potential problem is immobility in some regularly moving tissue.
\n
The advantages are the fast, painless, low-cost characteristics of the method, and the disadvantage is that it relies heavily on the examiner’s skills and knowledge. Conventional oral examination (COE) alone is insufficient for risk stratification. COE is generally useful for identifying lesions but not for subsequent clinical follow-up for treatment planning [20].
\n
Despite the importance of the methods described above as adjuvants in the process of diagnosis of suspected lesions, the biopsy is considered the gold standard for definitive diagnosis [20].
\n
All of these methods have their advantages and disadvantages and can be used in care to facilitate diagnosis. These noninvasive alternatives are not much disseminated in health services, and visual inspection under white light and palpation by a physician or dentist remain as the gold standard for screening of oral cancer. This procedure, however, has the limitation of being dependent on the examiner’s experience; this limitation underlies the development of more objective diagnostic techniques.
\n
Despite the scarcity of evidence about the abovementioned noninvasive methods as the diagnosis of a lesion front line, they can be useful in several situations. For instance, in cases where the biopsy is not a reasonable procedure, either for cost or complexity, most of these methods can make a difference.
\n
They used to be inexpensive, can be performed by less specialized professionals, are generally handled with lighter technology, and are more easily implemented in less resourceful regions and within primary health care [25, 26]. In these situations, the aim is to replace noninvasive techniques where a biopsy cannot be performed promptly. More, it can facilitate the screening of lesions in apparently healthy people, with or without risk factors for cancer, since it is a recommended noninvasive method that makes it possible to differentiate malignant to benign lesions. Despite the several possibilities of diagnostic methods, the rates still indicate that patients are diagnosed in advanced stages of cancer [25, 26, 37].
\n
These adjuvant diagnostic methods may help dentists better evaluate lesions suggestive of oral cancer before a definitive biopsy. The existing adjuvants such as toluidine blue, acetowhitening, and autofluorescence imaging are not much specific and, therefore, generally not recommended. Recently, new in vivo microscopy technologies such as high-resolution microendoscopy, optical coherence tomography, reflectance confocal microscopy, and multiphoton imaging have shown to offer promising improvements and more accurate diagnosis of these lesions and are not invasive procedures. The advantages of these technologies are that they allow the visualization of the microscopic characteristics used for histopathological evaluation at the moment of consultation, making the diagnosis faster, besides being painful or uncomfortable to patients [20].
\n
Other measures discussed are those related to the reorganization of health services, screening of risk groups, and awareness campaigns. These measures are used in many countries around the world, but the problem of late diagnosis is still a worldwide reality [38, 39].
\n
New strategies to approach the population and to identify suspicious lesions are paramount in the dissemination of information and for the increase of early diagnosed cases. The cooperation of primary health-care teams and not only of oral health professionals is essential for the fight against late diagnosis. Because other sectors of the health area often assist the population and can identify the risk, and even suspicious lesions, they also should be able to refer patients to the oral health sector. Thus, with all professionals working together in primary health care, identifying risk factors and suspicious lesions, and referring to the responsible sector, this collaborative work may bring a great positive gain for the population [14, 38].
\n
Below in a Table 2 is a summary of the methods discussed above.
\n
\n
\n
4.8 Considerations
\n
Considering the real problem of oral cancer worldwide, actions aimed at reducing the negative impact on society should be carried out with planning to achieve excellence of care to the population.
\n
Some factors lead to late diagnosis of oral cancer. Thus, such elements must be identified in each population so that health professionals can act to interfere with these factors, leading to better care for the community. Knowing population profile to be assisted is required, as much to identify factors that interfere with the diagnosis of the pathology as in the action planning.
\n
Although the biopsy is considered as the gold standard for definitive diagnosis, there are some constraints for your full application. This method is invasive and expensive, the results may take some days to be disclosed, and it requires specialized training, thus limiting its use for screening. Therefore, noninvasive methods are valuable, becoming more suitable in specific contexts.\n
Nomenclature
\n\nOC\n\n
oral cancer
\n\n\n\nHPV\n\n
human papilloma virus
\n\n\n\nIARC\n\n
International Agency for Research on Cancer
\n\n\n\nMPD\n\n
malignant cell lesions/disorders
\n\n\n\nCOE\n\n
conventional oral examination
\n\n\n\n
\n
\n
\n\n',keywords:"oral health, prevention, primary health care, secondary health care, oral cancer",chapterPDFUrl:"https://cdn.intechopen.com/pdfs/69234.pdf",chapterXML:"https://mts.intechopen.com/source/xml/69234.xml",downloadPdfUrl:"/chapter/pdf-download/69234",previewPdfUrl:"/chapter/pdf-preview/69234",totalDownloads:600,totalViews:0,totalCrossrefCites:0,totalDimensionsCites:1,totalAltmetricsMentions:0,impactScore:1,impactScorePercentile:57,impactScoreQuartile:3,hasAltmetrics:0,dateSubmitted:"May 20th 2019",dateReviewed:"August 21st 2019",datePrePublished:"November 28th 2019",datePublished:"May 13th 2020",dateFinished:"September 25th 2019",readingETA:"0",abstract:"Oral cancer is a public health problem because of its high morbidity and mortality, and when not treated in a timely manner, it is significantly mutilating, causing damage to the physical and psychological aspects of patients and directly interfering with their quality of life. Several factors influence the early diagnosis of this pathology, including lack of self-care related to oral health, especially among people with prolonged use of dental prosthesis; delayed perception of the lesion; delayed search for professional assistance since the lesion is noticed by the patient; lack of information about oral cancer, its risk and protective factors, and oral lesions that may be suggestive of cancer; lack of health promotion and prevention activities aimed at oral cancer; and lack of training in oral cancer among oral health professionals. These factors must be tackled to promote the timely diagnosis of this pathology. The use of reliable noninvasive diagnosis methods is also important because they can be easily made available in low resource settings, increasing the coverage of people who are under risk of developing oral cancer.",reviewType:"peer-reviewed",bibtexUrl:"/chapter/bibtex/69234",risUrl:"/chapter/ris/69234",book:{id:"9387",slug:"oral-diseases"},signatures:"Manoela Garcia Dias da Conceição, Ana Cláudia Figueiró and Vera Lucia Luiza",authors:[{id:"305759",title:"Dr.",name:"Manoela",middleName:"Garcia Dias",surname:"Da Conceição",fullName:"Manoela Da Conceição",slug:"manoela-da-conceicao",email:"manu_dias1984@yahoo.com.br",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:null},{id:"306004",title:"Dr.",name:"Vera Lucia",middleName:null,surname:"Luiza",fullName:"Vera Lucia Luiza",slug:"vera-lucia-luiza",email:"negritudesenior@gmail.com",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:null},{id:"310149",title:"Dr.",name:"Ana Cláudia",middleName:null,surname:"Figueró",fullName:"Ana Cláudia Figueró",slug:"ana-claudia-figuero",email:"anaclaudiafiguero@gmail.com",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:{name:"Escola Nacional de Saúde Pública",institutionURL:null,country:{name:"Brazil"}}}],sections:[{id:"sec_1",title:"1. Introduction",level:"1"},{id:"sec_2",title:"2. Factors related to late diagnosis of oral cancer",level:"1"},{id:"sec_3",title:"3. Prevention of oral cancer",level:"1"},{id:"sec_4",title:"4. Noninvasive methods for diagnosis of OC",level:"1"},{id:"sec_4_2",title:"4.1 Toluidine blue test",level:"2"},{id:"sec_5_2",title:"4.2 Exfoliative cytology",level:"2"},{id:"sec_6_2",title:"4.3 Fluorescence/autofluorescence",level:"2"},{id:"sec_7_2",title:"4.4 Contact endoscopy",level:"2"},{id:"sec_8_2",title:"4.5 In vivo microscopy",level:"2"},{id:"sec_9_2",title:"4.6 Tumor biomarkers",level:"2"},{id:"sec_10_2",title:"4.7 Oral inspection and palpation",level:"2"},{id:"sec_11_2",title:"4.8 Considerations",level:"2"}],chapterReferences:[{id:"B1",body:'\nWorld Health Organization. The World Oral Health Report 2003. Continuous improvement of oral health in the 21st century-the approach of the WHO Global Oral Health Programme. 2003\n'},{id:"B2",body:'\nToscano de Brito R, França Perazzo M, Santos Peixoto T, Weege-Nonaka CF, de Melo Brito Costa EM, Granville-Garcia AF. Profile of patients and factors related to the clinical staging of oral squamous cell carcinoma. Revista de Salud Pública Bogota. dezembro de. 2018;20(2):221-225\n'},{id:"B3",body:'\nChaturvedi P, Singh A, Chien C-Y, Warnakulasuriya S. Tobacco related oral cancer. BMJ. junho de. 2019;365:l2142\n'},{id:"B4",body:'\nFerlay J. Globocan 2012: Cancer Incidence and Mortality Worldwide. IARC; 2013\n'},{id:"B5",body:'\nFerlay J. Globocan 2018: Cancer Incidence and Mortality Worldwide. IARC; 2019\n'},{id:"B6",body:'\nMishra R. Biomarkers of oral premalignant epithelial lesions for clinical application. Oral Oncology. 06PY - 2012 de. 2012;48(7):578-584\n'},{id:"B7",body:'\nBhatt S, Isaac R, Finkel M, Evans J, Grant L, Paul B, et al. Mobile technology and cancer screening: Lessons from rural India. Journal of Global Health. 2018;8(2):020421-020421\n'},{id:"B8",body:'\nCarvalho AL, Singh B, Spiro RH, Kowalski LP, Shah JP. Cancer of the oral cavity: A comparison between institutions in a developing and a developed nation. janeiro de. 2004;26(1):31-38\n'},{id:"B9",body:'\nKowalski LP, Brentani MM, Coelho FRG. Bases da oncologia. São Paulo: Lemar; 2003\n'},{id:"B10",body:'\nRamos GHA, de Oliveira BV, Biasi LJ, Sampaio Júnior LA. Avaliação da citologia e do teste do azul de toluidina no diagnóstico dos tumores malignos da mucosa oral. Revista Brasileira de Cirurgia da Cabeça e Pescoço. 03PY - 2007 de. 2007;36(1):27-29\n'},{id:"B11",body:'\nda Silva MC, Marques EB, de Melo LC, de Bernardo JMP, Leite ICG. Fatores relacionados ao atraso no diagnóstico de câncer de boca e orofaringe em Juiz de Fora/MG. Revista Brasileira de Cancerologia. 2009;55(4):329-335\n'},{id:"B12",body:'\nRafieian N, Azimi S, Manifar S, Julideh H, ShirKhoda M. Is there any association between green tea consumption and the risk of head and neck squamous cell carcinoma: Finding from a case-control study. Archives of Oral Biology. 2019;98:280-284\n'},{id:"B13",body:'\nZhou H, Wu W, Wang F, Qi H, Cheng Z. Tea consumption is associated with decreased risk of oral cancer: A comprehensive and dose-response meta-analysis based on 14 case-control studies (MOOSE compliant). Med Baltimore. dezembro de. 2018;97(51):e13611\n'},{id:"B14",body:'\nAmorim NGC, Sousa AS, Alves SM. Prevenção e diagnóstico precoce do câncer bucal: Uma revisão de literatura. Revista Uningá. junho de. 2019;56(2):70-84\n'},{id:"B15",body:'\nNarvai PC, Frazão P. Saúde Bucal no Brasil muito além do céu da boca. Rio de Janeiro: Fiocruz; 2008. 148 p\n'},{id:"B16",body:'\nRangel EB, Lucietto DA, Stefenon L. Autopercepção de cirurgiões-dentistas sobre conhecimentos e práticas em relação ao câncer de boca. Revista Rede de Cuidados em Saúde. dezembro de. 2018;12(2):28-40\n'},{id:"B17",body:'\nTorres-Pereira CC, Angelim-Dias A, Melo NS, Lemos CA Jr, de Oliveira EMF. Abordagem do câncer da boca: Uma estratégia para os níveis primário e secundário de atenção em saúde Strategies for management of oral cancer in primary and secondary healthcare services. Cadernos de Saúde Pública. 2012;28(supl):s30-s39\n'},{id:"B18",body:'\nDias MG. Avaliação da Atenção em Saúde Bucal: contribuições para o controle do câncer de boca no município do Rio de Janeiro. Rio de Janeiro: Escola Nacional de Saúde Pública; 2018\n'},{id:"B19",body:'\nAl-Kaabi R, Gamboa ABO, Williams D, Marcenes W. Social inequalities in oral cancer literacy in an adult population in a multicultural deprived area of the UK. Journal of Public Health—Oxford Academic. 2016;38(3):474-482\n'},{id:"B20",body:'\nYang EC, Tan MT, Schwarz RA, Richards-Kortum RR, Gillenwater AM, Vigneswaran N. Noninvasive diagnostic adjuncts for the evaluation of potentially premalignant oral epithelial lesions: Current limitations and future directions. Oral Surgery, Oral Medicine, Oral Pathology, and Oral Radiology. abril de. 2018;125(6):670-681\n'},{id:"B21",body:'\nAlvarenga ML, Couto MG, de Ribeiro AO, Milagres RCM, Messora MR, Kawata LT. Avaliação do conhecimento dos cirurgiões-dentistas quanto ao câncer bucal. RFO UPF [Internet]. 04PY - 2012 de 2012;17(1). Disponível em: http://files.bvs.br/upload/S/1413-4012/2012/v17n1/a2980.pdf\n\n'},{id:"B22",body:'\nLeavell H, Clark EG. Preventive Medicine for the Doctor in His Community. MacGraw Hill: Nova York; 1965\n'},{id:"B23",body:'\nSilva, da AN, Senna, de MAA, Jorge RC, da Albuquerque DMS, de Queiroz TF. Promoção da Saúde. In: Fundamentos em Saúde Bucal Coletiva. Rio de Janeiro: Medbook; 2013. pp. 1-14\n'},{id:"B24",body:'\nBrasil Instituto Nacional de Câncer—INCA. A Situação do câncer no Brasil. INCA; 2006\n'},{id:"B25",body:'\nWalsh T, Liu JL, Brocklehurst P, Glenny AM, Lingen M, Kerr AR, et al. Clinical assessment to screen for the detection of oral cavity cancer and potentially malignant disorders in apparently healthy adults. Cochrane Database of Systematic Reviews. 12PY - 2013 de. 2013;11:CD010173-CD010173\n'},{id:"B26",body:'\nSweeny L, Dean NR, Magnuson JS, Carroll WR, Clemons L, Rosenthal EL. Assessment of tissue autofluorescence and reflectance for oral cavity cancer screening. Otolaryngology–Head and Neck Surgery. 12PY - 2011 de. 2011;145(6):956-960\n'},{id:"B27",body:'\nSharbatdaran M, Abbaszadeh H, Siadati S, Ranaee M, Hajian-Tilaki K, Rajabi-Moghaddam M. Assessment of oral cytological features in smokers and nonsmokers after application of toluidine blue. Diagnostic Cytopathology. março de. 2017;45(6):513-519\n'},{id:"B28",body:'\nDias MG, Figueiró AC, Luiza VL. Prevention and early diagnosis of oral cancer—A literature review. Revista Odonto Ciência. 25 de julho de. 2018;32(4):204\n'},{id:"B29",body:'\nLucena EE de S, Miranda AM, Araújo FA da C, Galvão CAB, de Medeiros AMC. Método de coleta e a qualidade do esfregaço de mucosa oral. Revista de Cirurgia e Traumatologia Buco Maxilo Facial. junho de. 2011;11(2):55-62\n'},{id:"B30",body:'\nRamos GHA, Tavares MR, Dedivitis RA, França CM, Oliveira BV, Pedruzzi PA. Endoscopia de contato (microestomatoscopia) nas lesões da boca e do lábio: Avaliação do método. Revista do Colégio Brasileiro de Cirurgiões. 2008;35(6):355-360\n'},{id:"B31",body:'\nPelc R, Masek V, Llopis-Torregrosa V, Bour P, Wu T. Spectral counterstaining in luminescence-enhanced biological Raman microscopy. Chemical Communications. julho de. 2019;55(57):8329-8332\n'},{id:"B32",body:'\nJiang W-P, Wang Z, Xu L-X, Peng X, Chen F. Diagnostic model of saliva peptide finger print analysis of oral squamous cell carcinoma patients using weak cation exchange magnetic beads. Julho de. 2015;35(3). Disponível em: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4613719/?tool=pubmed\n\n'},{id:"B33",body:'\nGuerrero-Preston R, Soudry E, Acero J, Orera M, Moreno-López L, Macía-Colón G, et al. NID2 and HOXA9 promoter hypermethylation as biomarkers for prevention and early detection in oral cavity squamous cell carcinoma tissues and saliva. Cancer Prevention Research (Phila). 07PY - 2011 de. 2011;4(7):1061-1072\n'},{id:"B34",body:'\nMankapure PK, Barpande SR, Bhavthankar JD, Mandale M. Serum big endothelin-1 as a biomarker in oral squamous cell carcinoma patients: An analytical study. Journal of Applied Oral Science. novembro de. 2015;23(5):491-496\n'},{id:"B35",body:'\nLingen MW. Screening for oral premalignancy and cancer: What platform and which biomarkers? Cancer Prevention Research (Phila). 09PY - 2010 de. 2010;3(9):1056-1059\n'},{id:"B36",body:'\nBrinkmann O, Kastratovic DA, Dimitrijevic MV, Konstantinovic VS, Jelovac DB, Antic J, et al. Oral squamous cell carcinoma detection by salivary biomarkers in a Serbian population. Oral Oncology. 02PY - 2011 de. 2011;47(1):51-55\n'},{id:"B37",body:'\nMehrotra R, Gupta DK. Exciting new advances in oral cancer diagnosis: Avenues to early detection. Head and Neck Oncology. 2011;3\n'},{id:"B38",body:'\nBulgareli JV, Diniz OCCF, de Faria ET, de Vazquez FL, Cortellazzi KL, Pereira AC. Prevenção e detecção do câncer bucal: planejamento participativo como estratégia para ampliação da cobertura populacional em idosos. Ciênc Saúde Coletiva. 12PY - 2013 de. 2013;18(12):3461-3473\n'},{id:"B39",body:'\nde Scheufen RC, Almeida FCS, da Silva DP, de Araujo ME, Palmiere M, Pegoretti T, et al. Prevenção e detecção precoce do câncer de boca: Screening em populações de risco. Pesquisa Brasileira em Odontopediatria e Clínica Integrada. 02PY - 2012 de. 2012;11(02). Disponível em: http://revista.uepb.edu.br/index.php/pboci/article/viewFile/1442/683\n\n'}],footnotes:[],contributors:[{corresp:"yes",contributorFullName:"Manoela Garcia Dias da Conceição",address:"manu_dias1984@yahoo.com.br",affiliation:'
Sergio Arouca National School of Public Health, ENSP, Oswaldo Cruz Foundation, FIOCRUZ, Brazil
Department of Drug Policy and Pharmaceutical Care, Sergio Arouca National School of Public Health, ENSP, Oswaldo Cruz Foundation, FIOCRUZ, Brazil
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1. Introduction
Coccidiosis is a worldwide disease caused by the Apicomplexa protozoa of Genus Eimeria [1]. This parasitic disease causes economic losses in poultry industry due to malabsorption, reduced weight gain (explained by a reduced feed intake and as well as nutrient absorption), increased mortality and the use of anticoccidial drugs and vaccines [2, 3] and remains as a major issue for poultry producers in a large number of countries including USA [4], costing for the global poultry industry over 3 billion dollars annually [5, 6]. However, subclinical coccidiosis (Coccidiasis) is more costly to producers than clinical coccidiosis because of impairment on FCR (Feed Conversion Ratio) and decreasing in BWG (Body Weight Gain), slightly resistance of anticoccidials as well as the difficultness in the diagnostic [6, 7]. The infection with Eimeria begins when the bird ingests sporulated oocyst, and the sporozoite replication occurs in the intestinal cells generating damage in the mucosa [8]. The infection is normally caused because of mixture of Eimeria species. Each species develops in different regions of the intestine and they can cause mild to severe grades of lesions [9]. There are several Eimeria species that infect chickens, but the most important are E. maxima, E. acervulina and E. tenella [3]. The infection process is very fast, from 4 to 7 days, depending on the species [9]. Previous studies reported that a coccidia challenge resulted in 29.5, 24.7, 18.8, and 96.2% reductions in the apparent ileal digestibility of DM, N, starch, and fat, respectively. Interestingly, the negative effects of coccidia challenge on fat digestibility suggested complex mechanisms involved [10]. The strategies to reduce the impact of poultry coccidiosis including the use of anticoccidial drugs, vaccination programs and immunomodulators (e.g., nutritional strategies and functional ingredients including phytobiotics and probiotics).
2. Coccidiosis and nutrition
From the nutritional standpoint, E. acervulina and E. maxima are the most influential species because of the strategic anatomical location being in duodenum (affecting the release of pancreatic juices) and jejunum intestine (nutrient absorption) respectively. E. acervulina and E. maxima significantly reduces the water consumption and feed intake simultaneously during acute coccidiosis, however the ratio of water intake to feed intake did not differ between chicks with coccidiosis and healthy birds. Hence, nutritional deficiencies and impaired nutrient absorption can occur following feed and water intake restrictions [11, 12].
Nutritional factors are key players in several steps of the coccidiosis disease. Firstly, as a susceptibility or protection component, secondly, during the process of infection and pathogenesis, and thirdly, in the recovery and compensatory growth of the bird.
Based on that, there are some facts pointing out that the presence of intestinal lesion scores correlates with decreases in average daily gain (ADG), energy consumption, retained energy and feed efficiency. Increments in maintenance is also reported while also seeing increase in excreted energy as well as reduced digestibility in most of the dietary nutrients [10]. The activation of immune response also occurs and requiring use of nutrients that cannot be addressed to the conversion of nutrients into meat, the key goal of broiler production. Therefore, it is paramount to figure out that any level of Eimeria infection is causing an evident, but difficult to estimate, loss in performance.
The use of good quality ingredients would aid in nutrients losses during infection and might help to reduce the potential secondary health issues. Nutrient dense diets during infection may be used to counteract performance losses and to enhance compensatory growth phase.
3. Malabsorption of nutrients
A reduction in apparent ileal digestibility of some nutrients including nitrogen, starch and fats in challenged birds with Eimeria has been reported [10]. As expected, coccidia challenged reduced starch, fat, and amino acids (AA) digestibility. Of these nutrients, fat digestibility was recognized as the most affected by the level of intestinal lesion [10]. Besides, an estimation of the intestinal damage and its impact on fat and AA digestibility was done. For each unit of impairment in total lesion score, digestibility was decreased by 16% and 3.8% respectively [10].
The first sign seen in birds which are infected with Eimeria is loss of pigmentation. In some countries, the coloring of the skin of broilers is an important factor when consumers choose the bird that they want to buy, as they directly associate yellowness or even goldenness with high quality, freshness, and country chickens [13, 14]. The extra cost for to achieve the level of desired pigmentation is high being between 8 to 10 percent of the total feed cost. Carotenoids are fat soluble and a loss of digestible energy specially of fats also affects its absorption in plasma. Besides, it is absorbed through the ciliary epithelium of the midgut and, for this take properly, a process of enzymatic hydrolysis of the xanthophylls, present in the diet in the form of fatty acid esters, must occur. During coccidia challenges, there is a decreasing up to 88% in serum levels of lutein. Some species of Eimeria cause desquamation and shortening of the villi of the intestinal mucosa and infects the sites of greatest pigment absorption in the intestine of birds [15].
The optimum pH of gastrointestinal tract is crucial for the action of digestive enzymes. However, coccidiosis infection has been responsible of causing malabsorption of nutrients which is related with the alteration of pH and morphological alteration including flattened villi and elongated [16]. The intestinal content was significantly lower in pH in birds after 5–9 days post infection of E. acervulina, E. mivati, E. maxima, E. necatrix and E. brunetti than in uninoculated control birds causing the impairment in absorption of nutrients [17]. Consequently, Eimeria-induced pH reductions likely impact nutrient digestion and absorption in the intestinal lumen. Also, pancreatic and mucosal brush border enzyme activity is affected at the tissue of infection, whereas brush border enzyme activity may be altered in uninfected intestinal regions compensating for losses in nutrient recovery [18]. Decreased activities of digestive enzymes located on the upper half of the villi, such as disaccharidases, indicate a damaged brush border with a decreased digestive absorptive capacity [16].
4. Eimeria infection and protein, amino acids and vitamin dietary levels
Nutritionists and parasitologists have argued for a long time the relation between coccidiosis and dietary protein levels. Early studies showed that chickens fed with high crude protein levels (between 20.5 to 22%) and high-vitamin A and B diets (40% more vitamin A, and about 20% more vitamin B of the requirements respectively) compared with low-protein (between 14.5 to 15.5%) and low-vitamin diet and exposed to equal numbers of E. tenella oocysts, had a consistently lower excretion of oocysts after 5 days of exposure and less than half the peak of oocyst shedding on day 7 post infection. Mortality percentage was 5% in the high-protein and high-vitamin A diet, compared to 23% in the low-protein and low-vitamin A diet. However, on the fifth and sixth day, the results were completely the opposite, suggesting that a high amount of protein and vitamin on the feed are overcoming the damage of acute coccidiosis. Nonetheless, during a chronic state of the disease, high levels of dietary proteins, caused an impairment of birds health [19]. As a shortcoming of this study, the effect of protein was not separated from the effects of vitamins.
Britton et al. (1964) showed the interaction between dietary protein levels and coccidiosis varying from 0 to 30% in intervals of 5% [20]. They found a significant reduction of mortality rate and in intestinal lesion score with 0 and 5% of dietary protein levels compared with 10 to 30%. In addition, the chickens fed with high amount of dietary protein showed more signs of coccidiosis such as blood in droppings and lethargy during first week of the challenge.
Furthermore, there is a relationship between the amount of dietary crude protein, trypsin activity and susceptibility to coccidiosis infection. To assess that, an experiment was conducted with chicks fed with diets varying protein levels (5, 20 and 30%) and its impact in intestinal trypsin activity. They found low levels of trypsin in 5% of protein fed compared with 20 and 30% protein [20]. A possible explanation is supported by the fact that when the birds are fed with high dietary protein levels, the production of trypsin and bile salts also increases as well. Thus, trypsin is one of the most important gastrointestinal enzymes for excystation of sporocysts from oocysts [21, 22]. The effect of trypsin intestinal levels and coccidia infection was also evidenced by others studies where chicks pancreatic ducts were ligated and there was no infection after orally challenge with Eimeria sporulated oocyst [23, 24].
At this regard, a study was conducted to determine the effect of feeding raw soybean containing 20.3 mg of trypsin inhibitor (TI) per g versus soybean meal of 2.2 TI mg/g in corn-soybean base diets in chickens on the risk to infection with 5 different species of Eimeria [25]. The key role of trypsin during excystation step in the coccidia cycle and its effect on the susceptibility of Eimeria infection was confirmed.
However, the continuous feeding of raw soybean in corn-soybean base diets also extremely developed weight depression and pancreatic hypertrophy, counteracting the positive effects of raw soybean on coccidiosis [25].
On the contrary, a high level of dietary protein was reported as a protection factor against E. tenella and E. acervulina infection. In contrast, mortality rate in E. tenella and the oocyst shedding in E. acervulina increase by feeding a diet with a 24% of crude protein. It has been also showed an improvement in the growth curve of chicks between 8 to 14 days post infection as compared with the first seven days post-infection and suggesting that compensatory growth can occur [26]. It would be appearing that the utilization of the maximum potential of compensatory growth requires highest levels in dietary protein or dense diets although more evidence is needed because most of the studies are only performed only seven days post infection disregarding the upcoming phases of broiler growth.
Adverse alterations of coccidiosis on key anatomical specific areas of the intestine such as the jejunum may affect protein digestibility. A compromised reduction in protein digestibility, using appearance of 14C in the blood of chicks fed labeled protein from cholera algae, was observed during an acute phase infection of E. necatrix-infected birds (jejunum) versus non-infected birds. Similarly, protein digestibility was not severe impaired in other species of Eimeria including E. acervulina and E. brunetti during the acute phase suggesting that duodenum, ileum, cecum, and colon were less important in protein digestion and absorption areas than jejunum. During the recovery period, a higher protein and absorption was observed above the absorption rates for uninfected birds [27].
Persia et al. (2006) reported a reduced performance, nitrogen-corrected apparent metabolizable energy (AMEn), and a total tract apparent amino acid digestibility (averaging of 12 percentage units for Thr, Val, Ile, Lys and Arg) with E. acervulina infection in birds inoculated with 5.0 x 105 sporulated oocyst in a time and dose response manner which was greater for acute versus chronic infections [28]. Ingredients such as fish meal and prebiotics mitigated the negative effects of coccidiosis lesions on cross bred chicks [28].
Parker et al. (2007) showed a decreased apparent total tract amino acid digestibility (ATTD, 8.4 percentage units) using Eimeria-vaccinated broilers challenged with a blend of Eimeria species compared with non-infected birds, being the branch chain amino acids (BCAA), and Thr and Cys the most affected [29]. Amerah and Ravindram (2015) showed that ATTD was most impacted for Ala, Cys, Ile, and Thr in broilers inoculated with a blend of sporulated Eimeria (1.8 × 105, 6 × 103, and 1.8 × 104E. acervulina, E. maxima, and E. tenella oocysts, respectively), showing a reduction of 13.4 percentage units in body weight gain (BWG) for all amino acids [10].
The effect of dietary protein on performance characteristics of vaccinated broilers subjected to clinical coccidiosis challenge has been examined. Lee et al. (2011) assessed the effect of dietary protein on the feed varying from 20 to 24% in coccidia-vaccinated chickens at the first day on broilers and subsequently challenged with different species and concentration of Eimeria (E. acervulina 6 x 105, E. maxima 4 x 105, and E. tenella 2 x 105) isolated from the field [30]. At day 21, an improvement in BWG and feed efficiency when increase dietary protein concentration regardless of vaccination status was found. However, vaccinated birds had a statistical trend to impair BWG and feed conversion ratio when was compared with non-vaccinated chicks before inoculation. After the Eimeria challenge (day 21 of age) and 0-to-6-day post infection, chicks fed with 24 percent crude protein diet had the highest feed efficiency. In the vaccinated group, birds fed the 24% crude protein diet had lower intestinal lesion scores than birds fed the 20% crude protein diet. No effects on compensatory growth were determined because of the end of trial at 6-day post inoculation [30].
Supplementation of some synthetic amino acids and their role in protection to coccidiosis infection are hypothesized that may overcome lesions in vaccinated birds. In this regard, Mussini et al. (2012) assessed the response of coccidiosis-vaccinated broilers to different levels of dietary glutamine (Glu, 0.5, 0.75, or 1%) during the immunity acquisition phases up to 28 days of age on performance and yield meat [31]. When dietary Glu was increased, BWG also increased concomitantly, regardless of the level of supplementation. On the age of 42 days, this observation was evidenced. Meat yield results did not show any effect with the Glu addition, however, a statistically trend (P = 0.07) in breast meat yield was observed. It is tempting to speculate, that Glu might be aiding to avoid muscle protein catabolism as well as supporting gastrointestinal and local immune system in the intestine.
In low protein diets, certain amino acids such as Gly, Ser and Pro have been supplemented by using gelatin as a source of conditionally essential amino acids to test their impact on compensatory growth in Eimeria-vaccinated broilers over a full grow-out period of 8 weeks. It was demonstrated that these amino acids improved performance because of their role in maintaining the mucosal barrier integrity [32].
The role of Thr (structural and prevalent amino acid of mucin) during broiler coccidiosis is controversial. Wils-Plotz (2013) showed a positive effect on growth performance when Thr was supplemented at 25% higher than dietary requirement. Nevertheless, Kidd et al. (2003) did not find any interaction between E. acervulina challenge and Thr supplementation meaning that broiler Thr dietary requirements are not increased during a mild infection of E. acervulina [33].
Arg supplementation has been hypothesized that play a key role on innate and humoral immune response during an Eimeria infection. Similarly, alleviate oxidative stress, improve antioxidant capacity, and attenuate the intestinal mucosa disruption. Thus, it might potentially increasing vaccine effectiveness and/or improve the responsiveness to field infections. An experiment varying different dietary levels of Arg and Vit E on the immune response against Eimeria challenge at day 14th of age with a field blend of Eimeria oocysts showed that heterophil and monocyte oxidative burst was improved with the concomitant inclusion of Arg and Vit E above the NRC requirements as well as serum levels of IgG and IgM [34]. In a recent publication, the effects of varying levels of Arg was measured in broilers challenged with a mix of Eimeria showing a promissory result where the levels ranging from 1.24 to 1.44 improving overall growth, intestinal integrity, and morphology [35].
Rochell et al. (2016) evaluated growth performance, ATTD, and plasma concentrations of amino acids, carotenoids, and α1-acid glycoprotein, an acute-phase reactants, in broilers from hatch to 21 d and inoculated with graded doses of E. acervulina oocysts [13]. BWG and feed efficiency dropped linearly when the doses of E. acervulina increased. Except for Trp and Gly, ATTD values decreased linearly or quadratically for all amino acids, relative to uninfected animals, by an average of 2.6 percentage units for birds inoculated with 1.0 x 106 oocysts.
Methionine (Met) is the first limiting amino acid in corn and soybean meal broiler diets that plays a major role in protein metabolism and has been highlighted as a crucial requirement for the immune system [36] and antioxidant defense system [37].
The impact of three dietary Met levels (0.45%, 0.56% and 0.68%) on alleviation of coccidia negative effects in broilers under various anticoccidial vaccination programs was examined in broilers from 22 to 42 d of age treated or vaccinated against coccidia after inoculation of E. tenella (5 x 104 sporulated oocyst) [38]. Dietary Met levels from 0.45 to 0.56% and 0.68% improved BWG and feed conversion ratio of broilers medicated against coccidia. However, dietary Met levels did not improve performance in vaccinated birds suggesting that Met levels could be benefiting the growth outcome in medicated chickens regardless on vaccinated chickens.
Two different Met sources and dietary levels of supplementation (non-supplemented, free Met and dipeptide Met) in coccidia challenged birds were examined on performance, gene expression related with immune responsiveness, antioxidant system and amino acid transport in Broiler diets [39]. No interaction between challenge and diet effects was found. However, BWG and feed conversion were improved (12.5 higher and 11.8% lower respectively) when free Met was supplemented compared to the non-supplemented birds. They also reported strong statistical differences comparing non-challenged versus challenged treatments for feed intake, BWG and feed conversion ratio. Coccidia challenge led higher amounts of oxidative substances in the jejunum of chickens 6 d post infection and decreased the gene expression of some amino acid transporters and immune response genes such as peptide transporter 1, toll-like receptor 5, interleukin-2 and occluding. Interferon gamma gene expression was also found increased [39].
5. How would nutrition help coccidiosis?
If producers are using coccidiosis vaccines, special diets could be designed to avoid peaks in oocyst production including the use of Glutamine [31].
The use of good quality ingredients is critical. High digestible feed ingredients where the nutrients are more available, the birds would have more probability to retain nutrients [40]. Also, to know the origin of feedstuffs avoiding the intake of pathogen microorganisms to the flock is crucial. Both microbiology and digestibility quality can aid in nutrients losses during infection and help reduce potential secondary health issues including necrotic enteritis, salmonellosis among others [41, 42].
Low protein diets may decrease the probability of infection but would affect BWG [25]. Thus, decreasing the amount of dietary protein it is not practical strategy. On the contrary, nutrient dense diets during infection may help with performance losses. However, excess of nutrients needs to be avoided on the lumen which may provide a substrate for entero-pathogens (i.e., surplus of protein in the hindgut for Clostridium perfringens proliferation) [43, 44].
The use of natural compounds including phytogenics and probiotics and beyond traditional strategies (ionophores and synthetics) have been considered to control coccidiosis challenges in the field or to reduce its severity [45, 46]. Phytobiotics have shown effectiveness against Eimeria (in-vitro and in vivo) and indirect positive outcomes related with boosting immune system and improving microbiota functions [45, 47]. Furthermore, phytobiotics affects fecal oocyst excretion and decrease intestinal lesion score exerting a direct effect on the parasite itself and altering its life cycle in the host [48, 49]. Anticoccidial effects of individual plants, plant extracts, and unidentified commercial plant-derived feed additives have been reviewed elsewhere [45, 49] but many of these studies were conducted using unknown blends and therefore repeatability and further investigation of physiologic pathways is scarce. Saponins by virtue of their surfactant properties have anti protozoal activity and they have membranolytic properties, they complex with cholesterol in protozoal membranes, causing cell lysis [50]. Saponins have shown immunomodulatory effects in broiler chickens challenged with a mix of Eimeria as evidenced by lymphocyte outcomes, changes in intestinal structure and alterations in cecal and duodenal inflammatory gene expression [51].
Feeding Probiotics as a replacement of traditional anticoccidials in the diet of broiler chickens might alleviate the impact of the disease and suggesting a coccidiostatic effect against E. tenella. One explanation is that probiotics aid to maintain intestinal health and integrity, act as an competitive exclusion principle and decrease the risk of coccidiosis [52]. It was also demonstrated a reduction in oocyst shedding of probiotics compared with Lasalocid. However, the role of feed supplementation of probiotics in performance did not show any improvement when was compared with Lasalocid [53].
An interesting study showed the positive effects of combine essential oils and vitamin D in vaccinated birds with coccidian oocyst of E. maxima and E. tenella [54]. An improvement in BWG and decreasing in FCR were observed when the essential oils and Vit D were supplemented compared with the non-vaccinated and non-treated birds. Furthermore, ATTD of dry matter tended to increase with the supplementation as well as an improvement in Lactobacillus counts.
6. Conclusions
Although coccidiosis has been the topic of a huge of research over the last decades, is still the major health issue on poultry industry and crucial questions remain answered. There are strategies to reduce or prevent the infection as well as to boost the compensatory growth after infection phase. In antibiotic free systems (ABF) coccidiosis control is requiring multifaceted approach. Nowadays, the strategy to control coccidiosis would not rely just only in coccidia vaccines or anticoccidials. Dietary interventions including protein and amino acids supplementations above the requirements may alleviate performance and immunological impairments in both vaccinated and/or unvaccinated broilers. Nonetheless, it varies according to characteristics of Eimeria, type of challenge, health status and environmental issues. Some studies are not considering long term effects such as compensatory growth and vaccines responsiveness during two or three consecutive flocks which may affect the parasite life cycle and immune response status. Nutritional requirements during coccidia infection can be improved by estimating the exact amount of dietary protein and amino acids required for a variety of intestinal functions (such as protein turnover, mucus production, and epithelial cells replacement, among others) and local immune supporting responses such as gut-associated lymphoid tissue. However, this type of information is scarce and further studies are needed to determine the exact role of each nutrient and their impact on performance and health in different production scenarios.
Conflict of interest
The authors declare no conflict of interest.
\n',keywords:"Eimeria infection, amino acids, digestibility, nutrient absorption, compensatory growth, immune response",chapterPDFUrl:"https://cdn.intechopen.com/pdfs/75810.pdf",chapterXML:"https://mts.intechopen.com/source/xml/75810.xml",downloadPdfUrl:"/chapter/pdf-download/75810",previewPdfUrl:"/chapter/pdf-preview/75810",totalDownloads:418,totalViews:0,totalCrossrefCites:1,dateSubmitted:"October 13th 2020",dateReviewed:"March 3rd 2021",datePrePublished:"March 19th 2021",datePublished:"July 7th 2021",dateFinished:"March 19th 2021",readingETA:"0",abstract:"Eimeria spp. are parasites specialized in invade and replicate in the intestine, causing coccidiosis, an enteric disease of major economic importance worldwide. The disease causes losses in production and high morbidity ranging from bloody enteritis, with high mortality, to being subclinical silent but affecting feed intake and efficiency. However, intestinal lesions of the infection vary, depending on the species of coccidia. The most important Eimeria species in poultry are: E. tenella, E. acervulina, E. maxima, E. necatrix, E. mitis, E. praecox and E. brunetti. All those species affect different anatomic sites of the intestine. Thus, they alter the homeostasis of the host reducing nutrient absorption and utilization. Nutritional factors are key players in several steps of the coccidiosis disease. Firstly, as a susceptibility or protection factor, secondly, during the process of infection and pathogenesis, and thirdly, in the recovery and compensatory growth of the bird. Otherwise, coccidiosis also triggers immune response in the intestine. To counter these complicated effects, there are nutritional strategies (including formulation of key amino acids, vitamins, short and medium chain fatty acids, prebiotics, enzymes, among others) that can be utilized to reduce the infection, alleviate the signs, and boost the compensatory growth after infection. This chapter review the impacts of coccidiosis in nutrition and discuss about of strategies to mitigate these risks.",reviewType:"peer-reviewed",bibtexUrl:"/chapter/bibtex/75810",risUrl:"/chapter/ris/75810",signatures:"Luis-Miguel Gómez-Osorio, Jenny-Jovana Chaparro-Gutiérrez and Sara López-Osorio",book:{id:"10361",type:"book",title:"Advances in Poultry Nutrition Research",subtitle:null,fullTitle:"Advances in Poultry Nutrition Research",slug:"advances-in-poultry-nutrition-research",publishedDate:"July 7th 2021",bookSignature:"Amlan Kumar Patra",coverURL:"https://cdn.intechopen.com/books/images_new/10361.jpg",licenceType:"CC BY 3.0",editedByType:"Edited by",isbn:"978-1-83969-001-3",printIsbn:"978-1-83969-000-6",pdfIsbn:"978-1-83969-002-0",isAvailableForWebshopOrdering:!0,editors:[{id:"310962",title:"Dr.",name:"Amlan",middleName:"Kumar",surname:"Patra",slug:"amlan-patra",fullName:"Amlan Patra"}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"}},authors:[{id:"335592",title:"Dr.",name:"Luis-Miguel",middleName:null,surname:"Gomez-Osorio",fullName:"Luis-Miguel Gomez-Osorio",slug:"luis-miguel-gomez-osorio",email:"lgomez@alura-ahn.com",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:null},{id:"335600",title:"Dr.",name:"Jenny-Jovanna",middleName:null,surname:"Chaparro-Gutierrez",fullName:"Jenny-Jovanna Chaparro-Gutierrez",slug:"jenny-jovanna-chaparro-gutierrez",email:"jenny.chaparro@udea.edu.co",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:{name:"University of Antioquia",institutionURL:null,country:{name:"Colombia"}}},{id:"335603",title:"Dr.",name:"Sara",middleName:null,surname:"Lopez-Osorio",fullName:"Sara Lopez-Osorio",slug:"sara-lopez-osorio",email:"sara.lopezo@udea.edu.co",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:null}],sections:[{id:"sec_1",title:"1. Introduction",level:"1"},{id:"sec_2",title:"2. Coccidiosis and nutrition",level:"1"},{id:"sec_3",title:"3. Malabsorption of nutrients",level:"1"},{id:"sec_4",title:"4. Eimeria infection and protein, amino acids and vitamin dietary levels",level:"1"},{id:"sec_5",title:"5. How would nutrition help coccidiosis?",level:"1"},{id:"sec_6",title:"6. Conclusions",level:"1"},{id:"sec_10",title:"Conflict of interest",level:"1"}],chapterReferences:[{id:"B1",body:'Richard W. Gerhold J. Overview of Coccidiosis in Poultry. MSD Veterinary Manual'},{id:"B2",body:'Hafez HM. Poultry coccidiosis: Prevention and control approaches. Arch fur Geflugelkd. 2008;72(1):2-7'},{id:"B3",body:'Chapman HD. Milestones in avian coccidiosis research : A review Citing articles via. Poult Sci. 2014;93:501-11'},{id:"B4",body:'Report of the USAHA Committee on Poultry and Other Avian Species [Internet]. Vol. 53. 2019. 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Actual and potential applications of and saponins in human and animal nutrition. J Anim Sci. 2000;77(E-Suppl):1'},{id:"B51",body:'Oelschlager ML, Rasheed MSA, Smith BN, Rincker MJ, Dilger RN. Effects of Yucca schidigera -derived saponin supplementation during a mixed Eimeria challenge in broilers. Poult Sci. 2019;98:3212-22'},{id:"B52",body:'Stringfellow K, Caldwell D, Lee J, Mohnl M, Beltran R, Schatzmayr G, et al. Evaluation of probiotic administration on the immune response of coccidiosis-vaccinated broilers. Poult Sci [Internet]. 2011;90(8):1652-8. Available from: http://dx.doi.org/10.3382/ps.2010-01026'},{id:"B53",body:'Giannenas I, Papadopoulos E, Tsalie E, Triantafillou E, Henikl S, Teichmann K, et al. Assessment of dietary supplementation with probiotics on performance, intestinal morphology and microflora of chickens infected with Eimeria tenella. Vet Parasitol [Internet]. 2012;188(1-2):31-40. Available from: http://dx.doi.org/10.1016/j.vetpar.2012.02.017'},{id:"B54",body:'Upadhaya SD, Cho SH, Chung TK, Kim IH. Anti-coccidial effect of essential oil blends and vitamin D on broiler chickens vaccinated with purified mixture of coccidian oocyst from Eimeria tenella and Eimeria maxima. Poult Sci [Internet]. 2019;98(7):2919-26. Available from: http://dx.doi.org/10.3382/ps/pez040'}],footnotes:[],contributors:[{corresp:"yes",contributorFullName:"Luis-Miguel Gómez-Osorio",address:"lgomez@alura-ahn.com",affiliation:'
Alura Animal Health and Nutrition, Bogota, Colombia
CIBAV Research Group, Faculty of Agrarian Sciences, University of Antioquia, UdeA, Colombia
CIBAV Research Group, Faculty of Agrarian Sciences, University of Antioquia, UdeA, Colombia
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From the fractography analysis (optical and scanning electron microscopy) of the both virgin and 50% recycled PC, it is evident that the fracture morphologies are very similar and they are indicative of ductile failure. It is observed that with the presence of temperature and humidity (60°C 90% RH) aging, tensile strength starts to drop over time but most importantly both 50% and 75% PCR grades showed similar aging behavior compared to virgin PC (10–13% strength degradation after 500 hours of aging). Reliability modelling showed comparable B10, Weibull Alpha and Weibull Beta values between Virgin PC and PCR grades after different aging conditions. 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I have over 300 citations for my published research in peer-reviewed journals.\nI have concentrated in my research on process modeling and simulation of chemical processes. I have organized and offered short courses on process modeling and simulation, process safety, risk assessment and analysis, and several others that are related to hydrocarbons processing and handling. I have been serving the community in many aspects at the various locations during my career. I have been a member of national committees (Standards and Codes, Non-CFC Standards, Jury Expert for Industrial Claims). I also supervised training at different industrial and professional entities. I have built a very good record with industry and received grants from different industries in Jordan (Jordan Phosphate Mines Co. and Arab Potash Co.) In the UAE I have received grants from (UAE University, General Holding Corporation for work on steel slag in cement manufacturing, Doosan Heavy Industries on desalination plants, Ministry of Presidential Affairs on Horizon for Petrochemicals in UAE, and recently from Emirates Foundation on photocatalytic conversion of CO2 back to hydrocarbons using TiO2 nanocatalyst and UV and/or visible light). I also received international grants from Japan Petroleum Institute and from Overseas Development Administration in Britain).\nI have been very competitive in getting research funding at the interdisciplinary and national levels and I am willing to engage myself in collaborative research, outstanding scholarly activities that will lead to national, regional and international recognition. In this regards, I have published 2 chapters in two books by Intech Open Access on “Ionic Liquids: Recycling for Reuse” in 2011 and \\Utilization of Ionic Liquids in Wood and Wood-Related Applications — A Review\\ in 2015",institutionString:null,institution:{name:"United Arab Emirates University",institutionURL:null,country:{name:"United Arab Emirates"}}},{id:"95620",title:"Dr.",name:"Dimitris S.",surname:"Achilias",slug:"dimitris-s.-achilias",fullName:"Dimitris S. Achilias",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/95620/images/system/95620.png",biography:"Dr. Dimitris S. Achilias is Professor of Polymer Chemistry and Technology, Department of Chemistry, Aristotle University of Thessaloniki (AUTh), Greece. He completed his BSc and Ph.D. in the Chemical Engineering Department, AUTH, in 1985 and 1991, respectively. 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Open Access publishing helps remove barriers and allows everyone to access valuable information, but article and book processing charges also exclude talented authors and editors who can’t afford to pay. The goal of our Women in Science program is to charge zero APCs, so none of our authors or editors have to pay for publication.
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Indeed, the resurgence of religions in all the continents is tangled in different ways to modernization processes. The main argument of this chapter is that this religious change toward pluralism can be fully understood in the context of multiple modernities theory, provided that it be revised and modified. The key understanding of changes must come from a better insight of popular religions worldwide. Latin American, Eastern Asia and Islam regions are good examples of popular forms of religious revitalization that contrasts with the Northern European case. New ways of producing sense and spiritual search in non-Western areas are framing specific relationships between religion and modernities and bringing about pluralisms. The interweaving of old and new religious traditions is accentuating interculturality and is generating great conditions for the emergence of new types of syncretism and/or sociocultural and even material (and violent) conflicts. The consequence is the development of religious patterns within societies that have a specific and distinctively form of modernity of their own.",book:{id:"6169",slug:"culture-and-identity",title:"Culture and Identity",fullTitle:"Culture and Identity"},signatures:"Cristian Parker",authors:[{id:"207097",title:"Dr.",name:"Cristián",middleName:null,surname:"Parker",slug:"cristian-parker",fullName:"Cristián Parker"}]},{id:"70743",doi:"10.5772/intechopen.90669",title:"Biotechnology and Cultural Heritage Conservation",slug:"biotechnology-and-cultural-heritage-conservation",totalDownloads:551,totalCrossrefCites:2,totalDimensionsCites:4,abstract:"The deterioration of cultural asset is induced by biological, chemical, and physical factors, influenced by anthropogenic activity and environmental conditions. In this study, the contribution of biotechnology is emphasized to define the conservation strategy, for a marble Fountain (Two Dragons, XV century) located in Palermo city center, based on an integrated approach and eco-friendly procedures. Biotechnological protocols are preliminarily applied as an integrated approach, based on microscopy observation, in vitro culture and genomic DNA analysis to recognize and characterize microbial communities. Several biological systems have been identified: green algae (Chlorella) and cyanobacteria (Cyanobium, Oscillatoria); bacteria (Arthrobacter, Bacillus, Micrococcus, Paracoccus); fungi (Alternaria, Aspergillus, Penicillium, Phoma, Fusarium, Cladosporium). In order to address biological colonization, the commercial Tea Tree Oil (Melaleuca alternifolia) and laboratory-distilled (Calamintha nepeta and Allium sativum) EOs, have been assayed by in vitro Agar disc diffusion, Well-plates diffusion, and Micro-dilution methods; the result allows to define the most appropriate EOs concentration to use. In a green conservation prospective, this study highlighted that EOs can potentially replace the traditional biocides, but the activity must be preliminary evaluated by centring the choose specifically on each microbial taxon identified.",book:{id:"6944",slug:"heritage",title:"Heritage",fullTitle:"Heritage"},signatures:"Franco Palla",authors:null},{id:"39099",doi:"10.5772/38850",title:"Japan’s University Education in Social Sciences and Humanities Under Globalization",slug:"education-and-research-of-social-science-and-humanities-in-japan-under-globalization",totalDownloads:1841,totalCrossrefCites:2,totalDimensionsCites:3,abstract:null,book:{id:"2301",slug:"social-sciences-and-cultural-studies-issues-of-language-public-opinion-education-and-welfare",title:"Social Sciences and Cultural Studies",fullTitle:"Social Sciences and Cultural Studies - Issues of Language, Public Opinion, Education and Welfare"},signatures:"Akiyoshi Yonezawa",authors:[{id:"120081",title:"Prof.",name:"Akiyoshi",middleName:null,surname:"Yonezawa",slug:"akiyoshi-yonezawa",fullName:"Akiyoshi Yonezawa"}]}],mostDownloadedChaptersLast30Days:[{id:"71819",title:"Significance in African Heritage",slug:"significance-in-african-heritage",totalDownloads:707,totalCrossrefCites:1,totalDimensionsCites:1,abstract:"Heritage professionals are at all times called upon to make significant judgments about heritage places/objects. There is a supposition therefore that heritage places or objects have intrinsic values that need to be discovered and assigned. This paper, using various examples from Africa, however, argues that values are not intrinsic to heritage but are a construct of heritage professionals/community, and therefore, a heritage place/object can have various values depending on who is making the judgment. It therefore follows that if values vary according to who is assigning them, then the significant/insignificant of a heritage place and object will also vary from one person/community to another. The paper concludes by arguing that significant/insignificant judgments are hegemonic constructions between contending forces, and therefore, it is difficult to have a universally accepted significant or insignificant judgment.",book:{id:"6944",slug:"heritage",title:"Heritage",fullTitle:"Heritage"},signatures:"Herman Ogoti Kiriama and Edith Nyangara Onkoba",authors:null},{id:"39079",title:"Are the Social Sciences Really- and Merely- Sciences?",slug:"are-the-social-sciences-really-and-merely-sciences",totalDownloads:2650,totalCrossrefCites:0,totalDimensionsCites:0,abstract:null,book:{id:"2301",slug:"social-sciences-and-cultural-studies-issues-of-language-public-opinion-education-and-welfare",title:"Social Sciences and Cultural Studies",fullTitle:"Social Sciences and Cultural Studies - Issues of Language, Public Opinion, Education and Welfare"},signatures:"Jeffrey Foss",authors:[{id:"121172",title:"Prof.",name:"Jeffrey",middleName:null,surname:"Foss",slug:"jeffrey-foss",fullName:"Jeffrey Foss"}]},{id:"73006",title:"Ideological Interaction Theory in Critical Discourse Analysis",slug:"ideological-interaction-theory-in-critical-discourse-analysis",totalDownloads:798,totalCrossrefCites:1,totalDimensionsCites:1,abstract:"Cultural and ideological references in critical discourse analysis aim at reframing decisions with exploring the cultural as well as ideological perspectives of the familiar and idiosyncratic styles. The chapter focuses on the development of the ideological interaction theory for connecting the discourse with language and personal thought. The concern criteria are ideology and the other social components like people, status, economy, media, and politics with their connectivity to the global situational trends. Therefore, the chapter surveys the personal traits as psychology, sociological surrounding, and cognitive efforts for the development of social as well as cultural interaction with the perspectives of individual influences.",book:{id:"6944",slug:"heritage",title:"Heritage",fullTitle:"Heritage"},signatures:"Yadu Prasad Gyawali",authors:null},{id:"58144",title:"Losing One’s Culture: The Narrative Identity of Nigerian Catholic Religious Sisters",slug:"losing-one-s-culture-the-narrative-identity-of-nigerian-catholic-religious-sisters",totalDownloads:878,totalCrossrefCites:0,totalDimensionsCites:0,abstract:"This chapter reports on part of the findings of a doctoral research focused on identity construction of Catholic religious sisters in the Church and in the wider Nigerian society. Primarily, the chapter interrogates how Catholic religious sisters negotiate their culture identity within the context of living religious life. Data were collected from 18 sister participants, who were purposefully recruited from two religious congregations across the different states of Nigeria. These included six temporary professed, six final professed and six leaders (including superiors/formators) representing the different categories of sisters that live religious life. The data were thematically analysed using the Dialogical Self Theory I-positions. The second sentence revealed tendencies for the participants to lose their cultural identity in terms of their struggles and sometimes compromises in identifying Western culture as the dominate culture of religious life. In this regard, the participants reported that their Nigerian communitarian culture of love, care and hospitality is regulated to the background. In response, this chapter calls for further research towards exploring the impact of culture on Catholic religious sisters’ expression of identity.",book:{id:"6169",slug:"culture-and-identity",title:"Culture and Identity",fullTitle:"Culture and Identity"},signatures:"Chika Eze, Graham C. Lindegger and Susan Rakoczy",authors:[{id:"216952",title:"Dr.",name:"Chika",middleName:null,surname:"Eze",slug:"chika-eze",fullName:"Chika Eze"}]},{id:"72251",title:"Public Diplomacy: Functions, Functional Boundaries and Measurement Methods",slug:"public-diplomacy-functions-functional-boundaries-and-measurement-methods",totalDownloads:831,totalCrossrefCites:2,totalDimensionsCites:2,abstract:"It is common practice when evaluating the effect of public diplomacy to associate it with soft power. This chapter challenges this view. It holds that, rather than turning soft power resources into soft power, the fundamental function of public diplomacy is to transform a country’s assets into soft power resources that will attract the target country. This chapter also shows that although public diplomacy performs such functions, it is not necessarily effective, especially in ‘high politics’ such as territorial security. But even where its effectiveness can be exerted, public diplomacy is subject to other distractions. Therefore, in order to measure accurately the real effect of public diplomacy, it is necessary to exclude the potential influence of these interfering variables by strengthening the rigor of research design.",book:{id:"6944",slug:"heritage",title:"Heritage",fullTitle:"Heritage"},signatures:"Cao Wei",authors:null}],onlineFirstChaptersFilter:{topicId:"270",limit:6,offset:0},onlineFirstChaptersCollection:[],onlineFirstChaptersTotal:0},preDownload:{success:null,errors:{}},subscriptionForm:{success:null,errors:{}},aboutIntechopen:{},privacyPolicy:{},peerReviewing:{},howOpenAccessPublishingWithIntechopenWorks:{},sponsorshipBooks:{sponsorshipBooks:[],offset:0,limit:8,total:null},allSeries:{pteSeriesList:[{id:"14",title:"Artificial Intelligence",numberOfPublishedBooks:9,numberOfPublishedChapters:87,numberOfOpenTopics:6,numberOfUpcomingTopics:0,issn:"2633-1403",doi:"10.5772/intechopen.79920",isOpenForSubmission:!0},{id:"7",title:"Biomedical Engineering",numberOfPublishedBooks:12,numberOfPublishedChapters:98,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2631-5343",doi:"10.5772/intechopen.71985",isOpenForSubmission:!0}],lsSeriesList:[{id:"11",title:"Biochemistry",numberOfPublishedBooks:27,numberOfPublishedChapters:287,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2632-0983",doi:"10.5772/intechopen.72877",isOpenForSubmission:!0},{id:"25",title:"Environmental Sciences",numberOfPublishedBooks:1,numberOfPublishedChapters:9,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2754-6713",doi:"10.5772/intechopen.100362",isOpenForSubmission:!0},{id:"10",title:"Physiology",numberOfPublishedBooks:11,numberOfPublishedChapters:139,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2631-8261",doi:"10.5772/intechopen.72796",isOpenForSubmission:!0}],hsSeriesList:[{id:"3",title:"Dentistry",numberOfPublishedBooks:8,numberOfPublishedChapters:129,numberOfOpenTopics:0,numberOfUpcomingTopics:2,issn:"2631-6218",doi:"10.5772/intechopen.71199",isOpenForSubmission:!1},{id:"6",title:"Infectious Diseases",numberOfPublishedBooks:13,numberOfPublishedChapters:106,numberOfOpenTopics:3,numberOfUpcomingTopics:1,issn:"2631-6188",doi:"10.5772/intechopen.71852",isOpenForSubmission:!0},{id:"13",title:"Veterinary Medicine and Science",numberOfPublishedBooks:10,numberOfPublishedChapters:103,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2632-0517",doi:"10.5772/intechopen.73681",isOpenForSubmission:!0}],sshSeriesList:[{id:"22",title:"Business, Management and Economics",numberOfPublishedBooks:1,numberOfPublishedChapters:12,numberOfOpenTopics:2,numberOfUpcomingTopics:1,issn:null,doi:"10.5772/intechopen.100359",isOpenForSubmission:!0},{id:"23",title:"Education and Human Development",numberOfPublishedBooks:0,numberOfPublishedChapters:0,numberOfOpenTopics:2,numberOfUpcomingTopics:0,issn:null,doi:"10.5772/intechopen.100360",isOpenForSubmission:!1},{id:"24",title:"Sustainable Development",numberOfPublishedBooks:0,numberOfPublishedChapters:9,numberOfOpenTopics:4,numberOfUpcomingTopics:1,issn:null,doi:"10.5772/intechopen.100361",isOpenForSubmission:!0}],testimonialsList:[{id:"13",text:"The collaboration with and support of the technical staff of IntechOpen is fantastic. 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Initial biochemical studies have been exclusively analytic: dissecting, purifying, and examining individual components of a biological system; in the apt words of Efraim Racker (1913 –1991), “Don’t waste clean thinking on dirty enzymes.” Today, however, biochemistry is becoming more agglomerative and comprehensive, setting out to integrate and describe entirely particular biological systems. The ‘big data’ metabolomics can define the complement of small molecules, e.g., in a soil or biofilm sample; proteomics can distinguish all the comprising proteins, e.g., serum; metagenomics can identify all the genes in a complex environment, e.g., the bovine rumen. 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Other positions she has held at the university include Vice-Dean of Master Programs, Vice-Dean of the Degree in Biology and Vice-Dean for Mobility and Enterprise and Engagement at the Faculty of Science (University of Alicante). She received her Bachelor in Biology in 1998 (University of Alicante) and her PhD in 2003 (Biochemistry, University of Alicante). She undertook post-doctoral research at the University of East Anglia (Norwich, U.K. 2004-2005; 2007-2008).\nHer multidisciplinary research focuses on investigating archaea and their potential applications in biotechnology. She has an H-index of 21. She has authored one patent and has published more than 70 indexed papers and around 60 book chapters.\nShe has contributed to more than 150 national and international meetings during the last 15 years. Her research interests include archaea metabolism, enzymes purification and characterization, gene regulation, carotenoids and bioplastics production, antioxidant\ncompounds, waste water treatments, and brines bioremediation.\nRosa María’s other roles include editorial board member for several journals related\nto biochemistry, reviewer for more than 60 journals (biochemistry, molecular biology, biotechnology, chemistry and microbiology) and president of several organizing committees in international meetings related to the N-cycle or respiratory processes.",institutionString:null,institution:{name:"University of Alicante",institutionURL:null,country:{name:"Spain"}}},editorTwo:null,editorThree:null},{id:"15",title:"Chemical Biology",coverUrl:"https://cdn.intechopen.com/series_topics/covers/15.jpg",isOpenForSubmission:!0,editor:{id:"441442",title:"Dr.",name:"Şükrü",middleName:null,surname:"Beydemir",slug:"sukru-beydemir",fullName:"Şükrü Beydemir",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y00003GsUoIQAV/Profile_Picture_1634557147521",biography:"Dr. Şükrü Beydemir obtained a BSc in Chemistry in 1995 from Yüzüncü Yıl University, MSc in Biochemistry in 1998, and PhD in Biochemistry in 2002 from Atatürk University, Turkey. He performed post-doctoral studies at Max-Planck Institute, Germany, and University of Florence, Italy in addition to making several scientific visits abroad. He currently works as a Full Professor of Biochemistry in the Faculty of Pharmacy, Anadolu University, Turkey. Dr. Beydemir has published over a hundred scientific papers spanning protein biochemistry, enzymology and medicinal chemistry, reviews, book chapters and presented several conferences to scientists worldwide. He has received numerous publication awards from various international scientific councils. He serves in the Editorial Board of several international journals. 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He is a member of the Turkish Biochemical Society, American Chemical Society, and German Genetics society. Dr. Ekinci published around ninety scientific papers, reviews and book chapters, and presented several conferences to scientists. He has received numerous publication awards from several scientific councils. 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He worked on the structure-function relationships of glycoconjugates and his main project was the investigations on the biological roles of the de-N-glycosylation enzymes (Endo-N-acetyl-β-D-glucosaminidase and peptide-N4-(N-acetyl-β-glucosaminyl) asparagine amidase). From 2002 he contributes to the understanding of the Blood-brain barrier functioning using proteomics approaches. He has published more than 70 papers. 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Since then, he has been working as an Adjunct Professor in the same Department at the University of Pavia. His research activity during the first years was primarily focused on the purification and structural characterization of enzymes from animal and plant sources. During this period, Prof. Iadarola familiarized himself with the conventional techniques used in column chromatography, spectrophotometry, manual Edman degradation, and electrophoresis). Since 1995, he has been working on: i) the determination in biological fluids (serum, urine, bronchoalveolar lavage, sputum) of proteolytic activities involved in the degradation processes of connective tissue matrix, and ii) on the identification of biological markers of lung diseases. In this context, he has developed and validated new methodologies (e.g., Capillary Electrophoresis coupled to Laser-Induced Fluorescence, CE-LIF) whose application enabled him to determine both the amounts of biochemical markers (Desmosines) in urine/serum of patients affected by Chronic Obstructive Pulmonary Disease (COPD) and the activity of proteolytic enzymes (Human Neutrophil Elastase, Cathepsin G, Pseudomonas aeruginosa elastase) in sputa of these patients. More recently, Prof. Iadarola was involved in developing techniques such as two-dimensional electrophoresis coupled to liquid chromatography/mass spectrometry (2DE-LC/MS) for the proteomic analysis of biological fluids aimed at the identification of potential biomarkers of different lung diseases. He is the author of about 150 publications (According to Scopus: H-Index: 23; Total citations: 1568- According to WOS: H-Index: 20; Total Citations: 1296) of peer-reviewed international journals. 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She gained considerable experience in developing and validating new methodologies whose applications allowed her to determine both the amount of biomarkers (Desmosine and Isodesmosine) in the urine of patients affected by COPD, and the activity of proteolytic enzymes (HNE, Cathepsin G, Pseudomonas aeruginosa elastase) in the sputa of these patients. Simona Viglio was also involved in research dealing with the supplementation of amino acids in patients with brain injury and chronic heart failure. She is presently engaged in the development of 2-DE and LC-MS techniques for the study of proteomics in biological fluids. The aim of this research is the identification of potential biomarkers of lung diseases. She is an author of about 90 publications (According to Scopus: H-Index: 23; According to WOS: H-Index: 20) on peer-reviewed journals, a member of the “Società Italiana di Biochimica e Biologia Molecolare,“ and a Consultant Reviewer for International Journal of Molecular Science, Journal of Chromatography A, COPD, Plos ONE and Nutritional Neuroscience.",institutionString:null,institution:{name:"University of Pavia",institutionURL:null,country:{name:"Italy"}}},editorThree:null}]},overviewPageOFChapters:{paginationCount:49,paginationItems:[{id:"80495",title:"Iron in Cell Metabolism and Disease",doi:"10.5772/intechopen.101908",signatures:"Eeka Prabhakar",slug:"iron-in-cell-metabolism-and-disease",totalDownloads:1,totalCrossrefCites:0,totalDimensionsCites:null,authors:null,book:{title:"Iron Metabolism - Iron a Double‐Edged Sword",coverURL:"https://cdn.intechopen.com/books/images_new/10842.jpg",subseries:{id:"17",title:"Metabolism"}}},{id:"81799",title:"Cross Talk of Purinergic and Immune Signaling: Implication in Inflammatory and Pathogenic Diseases",doi:"10.5772/intechopen.104978",signatures:"Richa Rai",slug:"cross-talk-of-purinergic-and-immune-signaling-implication-in-inflammatory-and-pathogenic-diseases",totalDownloads:7,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Purinergic System",coverURL:"https://cdn.intechopen.com/books/images_new/10801.jpg",subseries:{id:"17",title:"Metabolism"}}},{id:"81764",title:"Involvement of the Purinergic System in Cell Death in Models of Retinopathies",doi:"10.5772/intechopen.103935",signatures:"Douglas Penaforte Cruz, Marinna Garcia Repossi and Lucianne Fragel Madeira",slug:"involvement-of-the-purinergic-system-in-cell-death-in-models-of-retinopathies",totalDownloads:4,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Purinergic System",coverURL:"https://cdn.intechopen.com/books/images_new/10801.jpg",subseries:{id:"17",title:"Metabolism"}}},{id:"81756",title:"Alteration of Cytokines Level and Oxidative Stress Parameters in COVID-19",doi:"10.5772/intechopen.104950",signatures:"Marija Petrusevska, Emilija Atanasovska, Dragica Zendelovska, Aleksandar Eftimov and Katerina Spasovska",slug:"alteration-of-cytokines-level-and-oxidative-stress-parameters-in-covid-19",totalDownloads:8,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Chemokines Updates",coverURL:"https://cdn.intechopen.com/books/images_new/11672.jpg",subseries:{id:"18",title:"Proteomics"}}}]},overviewPagePublishedBooks:{paginationCount:27,paginationItems:[{type:"book",id:"7006",title:"Biochemistry and Health Benefits of Fatty Acids",subtitle:null,coverURL:"https://cdn.intechopen.com/books/images_new/7006.jpg",slug:"biochemistry-and-health-benefits-of-fatty-acids",publishedDate:"December 19th 2018",editedByType:"Edited by",bookSignature:"Viduranga Waisundara",hash:"c93a00abd68b5eba67e5e719f67fd20b",volumeInSeries:1,fullTitle:"Biochemistry and Health Benefits of Fatty Acids",editors:[{id:"194281",title:"Dr.",name:"Viduranga Y.",middleName:null,surname:"Waisundara",slug:"viduranga-y.-waisundara",fullName:"Viduranga Y. 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She has more than fifteen years of teaching and research experience. She has published more than 550 scientific publications/communications, including 15 books, 50 book chapters, 100 original research papers, 380 research communications in national and international conferences, and 12 patents. She is a member of the editorial board of five journals and acts as a reviewer for several national and international journals. Her research interests include microalgal biotechnology with an emphasis on microalgae-based products.",institutionString:"Universidade Federal de Santa Maria",institution:{name:"Universidade Federal de Santa Maria",institutionURL:null,country:{name:"Brazil"}}}]},{type:"book",id:"7953",title:"Bioluminescence",subtitle:"Analytical Applications and Basic Biology",coverURL:"https://cdn.intechopen.com/books/images_new/7953.jpg",slug:"bioluminescence-analytical-applications-and-basic-biology",publishedDate:"September 25th 2019",editedByType:"Edited by",bookSignature:"Hirobumi Suzuki",hash:"3a8efa00b71abea11bf01973dc589979",volumeInSeries:4,fullTitle:"Bioluminescence - Analytical Applications and Basic Biology",editors:[{id:"185746",title:"Dr.",name:"Hirobumi",middleName:null,surname:"Suzuki",slug:"hirobumi-suzuki",fullName:"Hirobumi Suzuki",profilePictureURL:"https://mts.intechopen.com/storage/users/185746/images/system/185746.png",biography:"Dr. Hirobumi Suzuki received his Ph.D. in 1997 from Tokyo Metropolitan University, Japan, where he studied firefly phylogeny and the evolution of mating systems. He is especially interested in the genetic differentiation pattern and speciation process that correlate to the flashing pattern and mating behavior of some fireflies in Japan. He then worked for Olympus Corporation, a Japanese manufacturer of optics and imaging products, where he was involved in the development of luminescence technology and produced a bioluminescence microscope that is currently being used for gene expression analysis in chronobiology, neurobiology, and developmental biology. Dr. Suzuki currently serves as a visiting researcher at Kogakuin University, Japan, and also a vice president of the Japan Firefly Society.",institutionString:"Kogakuin University",institution:null}]}]},openForSubmissionBooks:{},onlineFirstChapters:{},subseriesFiltersForOFChapters:[],publishedBooks:{},subseriesFiltersForPublishedBooks:[],publicationYearFilters:[],authors:{}},subseries:{item:{id:"22",type:"subseries",title:"Applied Intelligence",keywords:"Machine Learning, Intelligence Algorithms, Data Science, Artificial Intelligence, Applications on Applied Intelligence",scope:"This field is the key in the current industrial revolution (Industry 4.0), where the new models and developments are based on the knowledge generation on applied intelligence. 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His research lines are biometrics, biomedical signals and images, data mining, classification system, signal and image processing, machine learning, and environmental intelligence. He has researched in 52 international and Spanish research projects, some of them as head researcher. He is co-author of 4 books, co-editor of 27 proceedings books, guest editor for 8 JCR-ISI international journals, and up to 24 book chapters. He has over 450 papers published in international journals and conferences (81 of them indexed on JCR – ISI - Web of Science). He has published seven patents in the Spanish Patent and Trademark Office. He has been a supervisor on 8 Ph.D. theses (11 more are under supervision), and 130 master theses. He is the founder of The IEEE IWOBI conference series and the president of its Steering Committee, as well as the founder of both the InnoEducaTIC and APPIS conference series. He is an evaluator of project proposals for the European Union (H2020), Medical Research Council (MRC, UK), Spanish Government (ANECA, Spain), Research National Agency (ANR, France), DAAD (Germany), Argentinian Government, and the Colombian Institutions. He has been a reviewer in different indexed international journals (<70) and conferences (<250) since 2001. He has been a member of the IASTED Technical Committee on Image Processing from 2007 and a member of the IASTED Technical Committee on Artificial Intelligence and Expert Systems from 2011. \n\nHe has held the general chair position for the following: ACM-APPIS (2020, 2021), IEEE-IWOBI (2019, 2020 and 2020), A PPIS (2018, 2019), IEEE-IWOBI (2014, 2015, 2017, 2018), InnoEducaTIC (2014, 2017), IEEE-INES (2013), NoLISP (2011), JRBP (2012), and IEEE-ICCST (2005)\n\nHe is an associate editor of the Computational Intelligence and Neuroscience Journal (Hindawi – Q2 JCR-ISI). He was vice dean from 2004 to 2010 in the Higher Technical School of Telecommunication Engineers at ULPGC and the vice dean of Graduate and Postgraduate Studies from March 2013 to November 2017. 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