Surface sowed by potato crop in different districts of Sonora state (surface sampled to detect
\\n\\n
Dr. Pletser’s experience includes 30 years of working with the European Space Agency as a Senior Physicist/Engineer and coordinating their parabolic flight campaigns, and he is the Guinness World Record holder for the most number of aircraft flown (12) in parabolas, personally logging more than 7,300 parabolas.
\\n\\nSeeing the 5,000th book published makes us at the same time proud, happy, humble, and grateful. This is a great opportunity to stop and celebrate what we have done so far, but is also an opportunity to engage even more, grow, and succeed. It wouldn't be possible to get here without the synergy of team members’ hard work and authors and editors who devote time and their expertise into Open Access book publishing with us.
\\n\\nOver these years, we have gone from pioneering the scientific Open Access book publishing field to being the world’s largest Open Access book publisher. Nonetheless, our vision has remained the same: to meet the challenges of making relevant knowledge available to the worldwide community under the Open Access model.
\\n\\nWe are excited about the present, and we look forward to sharing many more successes in the future.
\\n\\nThank you all for being part of the journey. 5,000 times thank you!
\\n\\nNow with 5,000 titles available Open Access, which one will you read next?
\\n\\nRead, share and download for free: https://www.intechopen.com/books
\\n\\n\\n\\n
\\n"}]',published:!0,mainMedia:null},components:[{type:"htmlEditorComponent",content:'
Preparation of Space Experiments edited by international leading expert Dr. Vladimir Pletser, Director of Space Training Operations at Blue Abyss is the 5,000th Open Access book published by IntechOpen and our milestone publication!
\n\n"This book presents some of the current trends in space microgravity research. The eleven chapters introduce various facets of space research in physical sciences, human physiology and technology developed using the microgravity environment not only to improve our fundamental understanding in these domains but also to adapt this new knowledge for application on earth." says the editor. Listen what else Dr. Pletser has to say...
\n\n\n\nDr. Pletser’s experience includes 30 years of working with the European Space Agency as a Senior Physicist/Engineer and coordinating their parabolic flight campaigns, and he is the Guinness World Record holder for the most number of aircraft flown (12) in parabolas, personally logging more than 7,300 parabolas.
\n\nSeeing the 5,000th book published makes us at the same time proud, happy, humble, and grateful. This is a great opportunity to stop and celebrate what we have done so far, but is also an opportunity to engage even more, grow, and succeed. It wouldn't be possible to get here without the synergy of team members’ hard work and authors and editors who devote time and their expertise into Open Access book publishing with us.
\n\nOver these years, we have gone from pioneering the scientific Open Access book publishing field to being the world’s largest Open Access book publisher. Nonetheless, our vision has remained the same: to meet the challenges of making relevant knowledge available to the worldwide community under the Open Access model.
\n\nWe are excited about the present, and we look forward to sharing many more successes in the future.
\n\nThank you all for being part of the journey. 5,000 times thank you!
\n\nNow with 5,000 titles available Open Access, which one will you read next?
\n\nRead, share and download for free: https://www.intechopen.com/books
\n\n\n\n
\n'}],latestNews:[{slug:"webinar-introduction-to-open-science-wednesday-18-may-1-pm-cest-20220518",title:"Webinar: Introduction to Open Science | Wednesday 18 May, 1 PM CEST"},{slug:"step-in-the-right-direction-intechopen-launches-a-portfolio-of-open-science-journals-20220414",title:"Step in the Right Direction: IntechOpen Launches a Portfolio of Open Science Journals"},{slug:"let-s-meet-at-london-book-fair-5-7-april-2022-olympia-london-20220321",title:"Let’s meet at London Book Fair, 5-7 April 2022, Olympia London"},{slug:"50-books-published-as-part-of-intechopen-and-knowledge-unlatched-ku-collaboration-20220316",title:"50 Books published as part of IntechOpen and Knowledge Unlatched (KU) Collaboration"},{slug:"intechopen-joins-the-united-nations-sustainable-development-goals-publishers-compact-20221702",title:"IntechOpen joins the United Nations Sustainable Development Goals Publishers Compact"},{slug:"intechopen-signs-exclusive-representation-agreement-with-lsr-libros-servicios-y-representaciones-s-a-de-c-v-20211123",title:"IntechOpen Signs Exclusive Representation Agreement with LSR Libros Servicios y Representaciones S.A. de C.V"},{slug:"intechopen-expands-partnership-with-research4life-20211110",title:"IntechOpen Expands Partnership with Research4Life"},{slug:"introducing-intechopen-book-series-a-new-publishing-format-for-oa-books-20210915",title:"Introducing IntechOpen Book Series - A New Publishing Format for OA Books"}]},book:{item:{type:"book",id:"10385",leadTitle:null,fullTitle:"Advanced Applications of Hydrogen and Engineering Systems in the Automotive Industry",title:"Advanced Applications of Hydrogen and Engineering Systems in the Automotive Industry",subtitle:null,reviewType:"peer-reviewed",abstract:"The automobile industry is tremendously peculiar due to several strict requirements regarding functional reliability, safety standards, comfort level, high-volume production, and environmental limits. In addition, the industry is experiencing a disruptive evolution of modern vehicle research and design: electrification, connectivity, and autonomous driving. This book provides a robust overview of automotive engineering, including new proposals and the latest trends in road vehicle systems and sub-systems. Each chapter presents a rigorous analysis or a new solution in a clear and concise manner, such that professional and academic readers will appreciate both the theory dissertation and the industrial application.",isbn:"978-1-83968-298-8",printIsbn:"978-1-83968-297-1",pdfIsbn:"978-1-83968-299-5",doi:"10.5772/intechopen.91571",price:119,priceEur:129,priceUsd:155,slug:"advanced-applications-of-hydrogen-and-engineering-systems-in-the-automotive-industry",numberOfPages:226,isOpenForSubmission:!1,isInWos:null,isInBkci:!1,hash:"362b356f3ae4c3b5ab5a5fe69d92d270",bookSignature:"Luigi Cocco and Muhammad Aziz",publishedDate:"April 28th 2021",coverURL:"https://cdn.intechopen.com/books/images_new/10385.jpg",numberOfDownloads:3745,numberOfWosCitations:2,numberOfCrossrefCitations:1,numberOfCrossrefCitationsByBook:0,numberOfDimensionsCitations:7,numberOfDimensionsCitationsByBook:0,hasAltmetrics:0,numberOfTotalCitations:10,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"July 6th 2020",dateEndSecondStepPublish:"July 27th 2020",dateEndThirdStepPublish:"September 25th 2020",dateEndFourthStepPublish:"December 14th 2020",dateEndFifthStepPublish:"February 12th 2021",currentStepOfPublishingProcess:5,indexedIn:"1,2,3,4,5,6,7",editedByType:"Edited by",kuFlag:!1,featuredMarkup:null,editors:[{id:"112023",title:"Dr.",name:"Luigi",middleName:null,surname:"Cocco",slug:"luigi-cocco",fullName:"Luigi Cocco",profilePictureURL:"https://mts.intechopen.com/storage/users/112023/images/system/112023.jpg",biography:"Dr. Luigi Cocco is a professional engineer with fifteen years of experience in the automotive industry. He worked in the Electrical and Electronic R&D and Supply Quality departments of racing/luxury/premium Italian vehicle manufacturers, such as Ferrari F1 Team, Automobili Lamborghini, and Maserati. Currently, he is Head of Global Value Optimization for Passive Safety, Active Chassis and ADAS systems for all former FCA brands. After obtaining a master’s degree in Telecommunication Engineering, Dr. Cocco obtained a Ph.D. in Information Engineering. Since 2019, he has been a member of the Coordination Board of MotorVehicle University of Emilia-Romagna (MUNER). He has published several papers and three books: Modern Metrology Concerns (2012), New Trends and Developments in Metrology (2016), and Standards, Methods, and Solutions of Metrology (2018).",institutionString:"Maserati S.p.A.",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"0",totalChapterViews:"0",totalEditedBooks:"4",institution:null}],equalEditorOne:{id:"98160",title:"Associate Prof.",name:"Muhammad",middleName:null,surname:"Aziz",slug:"muhammad-aziz",fullName:"Muhammad Aziz",profilePictureURL:"https://mts.intechopen.com/storage/users/98160/images/system/98160.jpg",biography:"Dr. Muhammad Aziz is currently an Associate Professor at the Institute of Industrial Science, The University of Tokyo, Tokyo, Japan. He received a BEng, MEng, and DEng in Mechanical Engineering from Kyushu University, Japan, in 2004, 2006, and 2008, respectively. His research interests include power generation, renewable energy utilization, process modeling, smart grids, electric vehicles, batteries, and hydrogen production and utilization. He has published more than 125 peer-reviewed journals, 22 books and book chapters, and more than 200 proceedings. In addition, Dr. Aziz is also an active reviewer for more than sixty distinguished journals related to energy research.",institutionString:"The University of Tokyo",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"7",totalChapterViews:"0",totalEditedBooks:"1",institution:{name:"University of Tokyo",institutionURL:null,country:{name:"Japan"}}},equalEditorTwo:null,equalEditorThree:null,coeditorOne:null,coeditorTwo:null,coeditorThree:null,coeditorFour:null,coeditorFive:null,topics:[{id:"796",title:"Automation Engineering",slug:"automation-engineering"}],chapters:[{id:"73923",title:"Hybrid Steering Systems for Automotive Applications",doi:"10.5772/intechopen.94460",slug:"hybrid-steering-systems-for-automotive-applications",totalDownloads:485,totalCrossrefCites:0,totalDimensionsCites:1,hasAltmetrics:0,abstract:"The paper presents the results of the researches performed in order to find out the static and dynamic performances of some recent types of hybrid electrohydraulic servomechanisms. The new concept was generated by the needs of the modern aerospace technology called “flight by wire”, directed to the use of compact actuators composed mainly by a brushless motor driving a hydrostatic pump connected in a close loop with a hydraulic cylinder. The numerical simulations performed with Simcenter Amesim language pointed out the possibility of saving a lot of energy with this new concept, already used on civil airplanes and fighters. The new wave of autonomous driving generated a lot of combinations of electric and hydraulic components, according the peculiarities of applications. The authors studied the accuracy of the hybrid steering system of an articulated tractor based on a digital industrial servo valve combined with an ORBITROL standard unit. The structure of the last generation of the trucks hybrid steering systems was also studied, taking into account the real performances of each component. Finally, the effect of the strong penetration of the hybrid servo systems in the automotive manufacturing systems is assessed.",signatures:"Nicolae Vasiliu, Daniela Vasiliu, Constantin Drăgoi, Constantin Călinoiu and Toma Cojocaru-Greblea",downloadPdfUrl:"/chapter/pdf-download/73923",previewPdfUrl:"/chapter/pdf-preview/73923",authors:[{id:"328477",title:"Prof.",name:"Daniela",surname:"Vasiliu",slug:"daniela-vasiliu",fullName:"Daniela Vasiliu"},{id:"328478",title:"Dr.",name:"Toma",surname:"Cojocaru-Greblea",slug:"toma-cojocaru-greblea",fullName:"Toma Cojocaru-Greblea"},{id:"330949",title:"Prof.",name:"Nicolae",surname:"Vasiliu",slug:"nicolae-vasiliu",fullName:"Nicolae Vasiliu"},{id:"333794",title:"Dr.",name:"Constantin",surname:"Dragoi",slug:"constantin-dragoi",fullName:"Constantin Dragoi"},{id:"333795",title:"Dr.",name:"Constantin",surname:"Călinoiu",slug:"constantin-calinoiu",fullName:"Constantin Călinoiu"}],corrections:null},{id:"74236",title:"New Robust Control Design of Brake-by-Wire Actuators",doi:"10.5772/intechopen.94581",slug:"new-robust-control-design-of-brake-by-wire-actuators",totalDownloads:347,totalCrossrefCites:1,totalDimensionsCites:1,hasAltmetrics:0,abstract:"This chapter discusses control design of three different brake-by-wire actuators. The brakes studied include an Electro-Hydraulic brake with pressure modulation for wheel slip control, and two different Electro-Mechanical Brake configurations that directly use electric motors to control the movement of the caliper for wheel slip control. After modeling the actuators with the use of bond graphs, a cascaded control architecture is used to control these active systems. Individual controllers are designed using Youla robust control design method. Then, a feed-forward disturbance rejection is designed and added to the loops and its effectiveness is analyzed. Finally, a one-wheel model is used to compare these brake-by-wire systems in terms of stopping distance and actuator efforts.",signatures:"Ehsan Arasteh and Francis Assadian",downloadPdfUrl:"/chapter/pdf-download/74236",previewPdfUrl:"/chapter/pdf-preview/74236",authors:[{id:"279272",title:"Prof.",name:"Francis",surname:"Assadian",slug:"francis-assadian",fullName:"Francis Assadian"},{id:"327623",title:"Ph.D. Student",name:"Ehsan",surname:"Arasteh",slug:"ehsan-arasteh",fullName:"Ehsan Arasteh"}],corrections:null},{id:"74309",title:"Role of Bearings in New Generation Automotive Vehicles: Powertrain",doi:"10.5772/intechopen.94222",slug:"role-of-bearings-in-new-generation-automotive-vehicles-powertrain",totalDownloads:481,totalCrossrefCites:0,totalDimensionsCites:1,hasAltmetrics:0,abstract:"Modern Automobile powertrain’s technology is transforming to enable “connected, autonomous, shared and electric” (CASE). Modern automobiles are targeting to achieve the maximum vehicle uptime, utilization, and better total cost of ownership (TCO). Bearing is a vital component (sub-system) to achieve modern automobile’s performance targets. Bearings plays important role of performance enhancement of all the rotating parts in systems by carrying the load and facilitating transfer of torque. Bearings achieve its performance via correct selection of materials, manufacturing technologies, design optimized geometries, sealing, lubrication in addition to application specific features. In modern automobile passenger comfort is key consideration and role of bearing is critical to achieve lower system noise. This chapter focuses on building awareness of the bearing technical requirements for different aggregates and means to achieve the requirements for modern automobiles. Summary of 11,300 patent’s titles and customer voice analysis suggest the bearing development focus areas direction which are covered in this chapter. This chapter also introduces bearing technology research focus areas like reliability improvement, power-dense solutions, integrated functions, friction optimization, sealing/lubrication solutions, adoption of sensors, and also special application-specific eMotors bearings. Modern electronic technologies integrated with bearings are performing the critical role of powertrain health monitoring in the vehicle. However, bearings are having furthermore potential to contribute and enhance the performance of modern automobiles in near future.",signatures:"Maruti Khaire",downloadPdfUrl:"/chapter/pdf-download/74309",previewPdfUrl:"/chapter/pdf-preview/74309",authors:[{id:"326516",title:"Ph.D.",name:"Maruti",surname:"Khaire",slug:"maruti-khaire",fullName:"Maruti Khaire"}],corrections:null},{id:"73339",title:"Generation and Relaxation of Residual Stresses in Automotive Cylinder Blocks",doi:"10.5772/intechopen.93664",slug:"generation-and-relaxation-of-residual-stresses-in-automotive-cylinder-blocks",totalDownloads:376,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"There is direct proportionality between ultimate tensile stress (UTS) and residual stresses (RS). Residual stresses gradually decrease with decreasing cooling/quenching rates. Quenching in cold water develops highest, whereas air cooling produces lowest, residual stresses. Significant increase in RS is observed in specimens with low dendrite arm spacing (high solidification rate), while lower residual stresses are measured in specimens with high dendrite arm spacing (low solidification rate). For I-4 and V-6 engine blocks, there is refinement in microstructure due to the increase in solidification rate along the cylinder length. The developed residual stresses are normally tensile in both engine types. Air cooling following solution heat treatment produces higher RS compared to warm water and cold water quenching. Solution heat treatment and freezing lead to maximum RS relaxation where 50% of the stresses are reduced after the solution heat treatment step. Aging time and temperature are directly proportional to the residual stresses relaxation. Relaxation of RS also depends on the geometry and size of the workpiece. It should be mentioned here that the I-4 and V-6 cylinder blocks were provided by Nemak-Canada (Windsor-Ontario-Canada). Residual stress measurements technique and procedure are typical of those used by the automotive industry in order to provide reliable data for industrial applications supported by intensive experiments.",signatures:"Serageldin Salem Mohamed, Agnes M. Samuel, Herbert W. Doty, Salvador Valtierra and Fawzy H. Samuel",downloadPdfUrl:"/chapter/pdf-download/73339",previewPdfUrl:"/chapter/pdf-preview/73339",authors:[{id:"178918",title:"Dr.",name:"Fawzy H.",surname:"Samuel",slug:"fawzy-h.-samuel",fullName:"Fawzy H. Samuel"},{id:"184917",title:"Dr.",name:"Herbert",surname:"Doty",slug:"herbert-doty",fullName:"Herbert Doty"},{id:"320504",title:"Dr.",name:"Agnes Marie",surname:"Samuel",slug:"agnes-marie-samuel",fullName:"Agnes Marie Samuel"},{id:"327921",title:"Dr.",name:"Serag",surname:"Mohamed",slug:"serag-mohamed",fullName:"Serag Mohamed"},{id:"327922",title:"Dr.",name:"Salvador",surname:"Valtierra",slug:"salvador-valtierra",fullName:"Salvador Valtierra"}],corrections:null},{id:"74124",title:"Quality and Trends of Automotive Fuels",doi:"10.5772/intechopen.94167",slug:"quality-and-trends-of-automotive-fuels",totalDownloads:306,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"Automotive engines are designed to convert chemical energy to mechanical energy. The efficiency of this conversion is governed by thermodynamics. The two most common engines utilize gas oil and gasoline fuels for this purpose. However, the combustion processes are radically different. The combustion sequence and relative characteristics for both engine types will be discussed. Due to different combustion requirements, the fundamental properties of both fuels will also be examined as these are significantly different for the two fuel types. The main fuel properties discussed are energy density, stability, fluidity, corrosion, contaminants, safety, wear and environmental aspects. Also, with the advent of various renewable components in both fuels, new trends are emerging for both fuel quality assessments as these are molecularly distinct from their crude oil counterparts.",signatures:"David Andrew Gauci and Panagiotis Arkoudeas",downloadPdfUrl:"/chapter/pdf-download/74124",previewPdfUrl:"/chapter/pdf-preview/74124",authors:[{id:"326550",title:"Dr.",name:"Panagiotis",surname:"Arkoudeas",slug:"panagiotis-arkoudeas",fullName:"Panagiotis Arkoudeas"},{id:"326639",title:"Dr.",name:"David",surname:"Gauci",slug:"david-gauci",fullName:"David Gauci"}],corrections:null},{id:"74920",title:"Light Weight Complex Metal Hydrides for Reversible Hydrogen Storage",doi:"10.5772/intechopen.95808",slug:"light-weight-complex-metal-hydrides-for-reversible-hydrogen-storage",totalDownloads:360,totalCrossrefCites:0,totalDimensionsCites:2,hasAltmetrics:0,abstract:"We have investigated the complex metal hydrides involving light weight elements or compounds for the reversible hydrogen storage. The complex hydrides are prepared via an inexpensive solid state mechanochemical process under reactive atmosphere at ambient temperatures. The complex metal hydride, LiBH4 with different mole concentrations of ZnCl2 were characterized for the new phase formation and hydrogen decomposition characteristics of Zn(BH4)2. Furthermore, the complex metal hydride is destabilized using the addition of nano MgH2 for the reversible hydrogen storage characteristics. The structural, microstructural, surface, and other physicochemical behaviors of these lightweight complex metal hydrides have been studied via various metrological tools such as x-ray diffraction, Fourier transform infrared spectroscopy, thermal programed desorption, and PCT hydrogen absorption methods.",signatures:"Sesha Srinivasan, Luis Rivera, Diego Escobar and Elias Stefanakos",downloadPdfUrl:"/chapter/pdf-download/74920",previewPdfUrl:"/chapter/pdf-preview/74920",authors:[{id:"327581",title:"Dr.",name:"Sesha",surname:"Srinivasan",slug:"sesha-srinivasan",fullName:"Sesha Srinivasan"},{id:"345553",title:"Dr.",name:"Luis",surname:"Rivera",slug:"luis-rivera",fullName:"Luis Rivera"},{id:"345554",title:"Dr.",name:"Diego",surname:"Escobar",slug:"diego-escobar",fullName:"Diego Escobar"},{id:"345555",title:"Dr.",name:"Elias",surname:"Stefanakos",slug:"elias-stefanakos",fullName:"Elias Stefanakos"}],corrections:null},{id:"74097",title:"Hydrogen Storage: Materials, Kinetics and Thermodynamics",doi:"10.5772/intechopen.94300",slug:"hydrogen-storage-materials-kinetics-and-thermodynamics",totalDownloads:380,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"The need for cleaner sources of energy has become a serious need now more than ever due to the rising effects of fossil fuels on the environment. Technological advancement in society today has necessitated the need for fast and robust materials that will match the speed at which society is moving forward. Hydrogen as an alternative source, has garnered a lot of attention due to its zero emission characteristic. In this chapter, a background on hydrogen storage and its impact on the ‘envisaged green environment’ is discussed. Graphene and borohydrides hydrogen storage materials are reviewed extensively and the kinetic models thereof. Furthermore, the reaction mechanism of graphene nanocomposites is also discussed.",signatures:"Athule Ngqalakwezi and Diakanua Bevon Nkazi",downloadPdfUrl:"/chapter/pdf-download/74097",previewPdfUrl:"/chapter/pdf-preview/74097",authors:[{id:"326297",title:"M.Sc.",name:"Athule",surname:"Ngqalakwezi",slug:"athule-ngqalakwezi",fullName:"Athule Ngqalakwezi"},{id:"333154",title:"Prof.",name:"Diakanua Bevon",surname:"Nkazi",slug:"diakanua-bevon-nkazi",fullName:"Diakanua Bevon Nkazi"}],corrections:null},{id:"74420",title:"Hydrogen Fuel Cell Implementation for the Transportation Sector",doi:"10.5772/intechopen.95291",slug:"hydrogen-fuel-cell-implementation-for-the-transportation-sector",totalDownloads:703,totalCrossrefCites:0,totalDimensionsCites:2,hasAltmetrics:0,abstract:"Global transportation possesses have compelling rationales for reducing the consumption of oil, emissions of carbon dioxide, and noise pollution. Transitions to alternative transportation technologies such as electric vehicles (EVs) have gained increased attention from the automotive industries. A fuel cell electric vehicle (FCEV) occupying a hydrogen engine is one of the most stupendous technologies, since it is suitable for a large-scale transportation. However, its performance limitations are in question due to voltage degradation in long term operations through steady conditions under constant load and dynamic working conditions. Other drawbacks of using fuel cells in EVs are energy balances and management issues necessary for vehicle power and energy requirements. An efficient solution to accommodate driving behavior like dynamic loads comprises of hybridizing PEMFCs with energy storage devices like supercapacitors and batteries. This opening chapter reviews the projected gist of FCEV status; considers the factors that are going to affect how FCEVs could enter commercialization, including the importance of fuel cells for EV technologies; the degradation diagnoses using accelerated stress test (AST) procedures; FCEV hybridization; and the contribution of an energy storage device for charging EVs. The article also addresses case studies relating to material degradation occurring from driving behavior. Information about material degradation can be compiled into a database for the improvement of cell component performance and durability, leading to the creation of new materials and new fuel cell hybridization designs. To support the growth of EV technologies, an energy storage is required for the integrated alternative electricity generations. A redox flow battery is considered as a promising candidate in terms of attractive charging station for EVs or HEVs.",signatures:"Rungsima Yeetsorn and Yaowaret Maiket",downloadPdfUrl:"/chapter/pdf-download/74420",previewPdfUrl:"/chapter/pdf-preview/74420",authors:[{id:"328663",title:"Assistant Prof.",name:"Rungsima",surname:"Yeetsorn",slug:"rungsima-yeetsorn",fullName:"Rungsima Yeetsorn"},{id:"328848",title:"Ms.",name:"Yaowaret",surname:"Maiket",slug:"yaowaret-maiket",fullName:"Yaowaret Maiket"}],corrections:null},{id:"73891",title:"Quantum Calculations to Estimate the Heat of Hydrogenation Theoretically",doi:"10.5772/intechopen.93955",slug:"quantum-calculations-to-estimate-the-heat-of-hydrogenation-theoretically",totalDownloads:312,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"Standard enthalpies of hydrogenation of 29 unsaturated hydrocarbon compounds were calculated in the gas phase by CCSD(T) theory with complete basis set cc-pVXZ, where X = DZ, TZ, as well as by complete basis set limit extrapolation. Geometries of reactants and products were optimized at the M06-2X/6-31g(d) level. This M06-2X geometries were used in the CCSD(T)/cc-pVXZ//M06-2X/6-31g(d) and cc-pV(DT)Z extrapolation calculations. (MAD) the mean absolute deviations of the enthalpies of hydrogenation between the calculated and experimental results that range from 8.8 to 3.4 kJ mol−1 based on the Comparison between the calculation at CCSD(T) and experimental results. The MAD value has improved and decreased to 1.5 kJ mol−1 after using complete basis set limit extrapolation. The deviations of the experimental values are located inside the “chemical accuracy” (±1 kcal mol−1 ≈ ±4.2 kJ mol−1) as some results showed. A very good linear correlations between experimental and calculated enthalpies of hydro-genation have been obtained at CCSD(T)/cc-pVTZ//M06-2X/6-31g(d) level and CCSD(T)/cc-pV(DT)Z extrapolation levels (SD =2.11 and 2.12 kJ mol−1, respectively).",signatures:"Ali Amir Khairbek",downloadPdfUrl:"/chapter/pdf-download/73891",previewPdfUrl:"/chapter/pdf-preview/73891",authors:[{id:"326743",title:"Ph.D. Student",name:"Ali",surname:"Khairbek",slug:"ali-khairbek",fullName:"Ali Khairbek"}],corrections:null}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"},subseries:null,tags:null},relatedBooks:[{type:"book",id:"1547",title:"Modern Metrology Concerns",subtitle:null,isOpenForSubmission:!1,hash:"27edf8c2b79a9c6ae0c3d87ca8cae49c",slug:"modern-metrology-concerns",bookSignature:"Luigi Cocco",coverURL:"https://cdn.intechopen.com/books/images_new/1547.jpg",editedByType:"Edited 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With a radical change in international trade, and with the movement of vegetative material and sowing of these plant products, pests are dispersing throughout the world, becoming a more complex problem. Even when efforts are made, in the first instance to measure the introduction of diseases from other countries and secondly to prevent their dispersion from primary inoculum sources, the negative aspects mentioned above have, to a certain degree, caused losses and major disruptions in some regions to national agriculture, so it is advisable to have the necessary measures to prevent the entry and secondly the control and dispersion of these biological agents harmful to plants [1]. In the Mexican Republic, specifically in northwestern Mexico, the area targeted for the cultivation of vegetables (mainly tomato, potato, chili, and watermelon) has increased considerably in recent years [2]. Mexico does not produce quality seed, it obliges producers to acquire tuber of foreign origin, mainly from the United States, since the varieties acquired produce fruits that meet the characteristics preferred by the consumer and also cause the tuber volumes to enter the country which is a gateway for microorganisms of quarantine importance such as
This research aims to expand knowledge of the situation that occurs in the detection of bacteria of quarantine importance, in addition to updating and reaffirming its null presence in the agricultural areas of the state, and later extend to the interior of the country. In addition, this screening study is aimed at involving producers with a new production scheme under phytosanitary conditions in order to safeguard our national agriculture.
On the other hand, in recent years, there has been a growing interest in the use of biologically active compounds extracted from plant species that have the ability to eliminate pathogenic microorganisms by themselves, mainly due to the resistance that microorganisms have developed to antibiotics [3]. In addition, agriculture in the new millennium must establish new control alternatives that produce a lower environmental impact, as day-to-day increases in the percentage of consumers who demand healthy and chemical-free food [4]. Therefore, the importance of knowing new control strategies arises, especially those that have a sustainable aspect. Based on the above, the need to evaluate bactericidal products to perform tests of antimicrobial activity against
According to the abovementioned details, the principal goals were to detect the presence of
The research was carried out in two stages: the first one consisted in the increase of
It was initiated with the increase of
Pathogenicity tests (PPs) were developed in order to familiarize themselves with the symptomatology of
For the process of identification of
In the second stage, farmers donated tubers from Canada and the USA; the tuber, which is consumed by human and used as a seed, was sampled in commercial stores in Sonora state. Also, fields of potato crop were sampled in three stages: seedling, flowering stage, and physiological maturity when the plants produced tubers considered to be cut and later for sale. It should be noted that seedlings, leaves, or fruits of plants showing a symptomatology similar to that of
Detection of Rs in tuber. According to Rueda [1], each tuber sample, consisting of 10 tubers from each batch of cooperating producers, weighed separately, washed in running water for 30 min, and placed in plastic trays with a capacity of 2 L. Each tray, with its respective sample of tuber, is left with an amount of 100 ml of distilled water, and each of these trays was added 2 ml of buffer solution phosphatase with a pH = 7. The water-phosphatase mixture containing each tuber sample is called a “mother suspension.” The trays were incubated for 12 hours in cooling at 4°C in order to release the bacteria to the stock suspension. After the incubation, 10 ml was taken from suspension of each of the trays, four dilutions were made to such suspension (10:1, 10:2, 10:3, 10:4), and the last dilution of 0.1 ml was taken and sowed in specific culture medium in Petri dishes by the rod dispersion method The media were incubated for 7 days at 34°C. The inoculated media were then incubated at a temperature of 35°C for 7 days [1].
Detection of Rs in vegatitive material sampled (tuber, sedling, leaf and fruit), by ELISA technique. For the detection of
Detection of
Pathogenicity tests to positive bacteria with the different methods of detection. For the reaffirmation of
This stage which consisted of the evaluation of the antibacterial activity of two essential oils was carried out in the laboratory microbiology and mycotoxins of the Department of Research and Postgraduate in Food of the University of Sonora, in the city of Hermosillo, Sonora, Mexico. The experiment was carried out in vitro under controlled conditions at 30 °C and 90% humedity.
The bacterial strain used in the study was
The bacterial strain was grown in a culture of 24 hours at 30°C in Nutrient Broth (Difco, Sparks, MD) (extract of 3.0 g and peptone of 5.0 g) and adjusted to a concentration of 108 CFU/ml with phosphate-buffered saline (PBS). The bacterial inoculum was massively planted on dextrose and potato agar plates using a sterile cotton swab to achieve uniform microbial growth [6].
Once the plates were inoculated with the bacteria, filter paper disks of approximately 10 mm in diameter were placed in the center of the dish, in which different amounts of the essential oils were applied.
Essential oils were prepared at different concentrations using 70% ethyl alcohol as diluent. The concentrations used were 1:1, 1:5, and 1:10. Aseptically, 7.5, 10, and 15 μl of each of the concentrations of the essential oils were placed on the filter paper disks. Seventy percent alcohol was used in one of the filter paper disks as a negative control to discard the antimicrobial activity of the same. In addition, a disk of streptomycin (10 μg/disk) and one ampicillin (10 μg/disk) were used as the reference control. After impregnating the disks with the respective treatment, the plates were incubated at 30°C for 24 hours. After the incubation period, bacterial growth inhibition halos were measured in millimeters using a ruler. Analyses were carried out in triplicate.
The experimental design was trifactorial A
When testing for pathogenicity for
Regarding the sowing of “bacterial suspensions” obtained from the pathogenicity test on media Mannitol salt agar (SMSA), and tetrazolium chloride (TZC) cultures, the results indicate that on the TZC medium, developed colonies showed a pinkish-white color, while on the SMSA medium, colonies of developed bacteria showed an irregular shape, of white color and with centers of pink color after 72 hours of incubation at 30°C. A similar result was obtained for those PPs that were inoculated with the positive control (
By ELISA confirmation, a positive result was obtained for the control strain (
Sampling of potatoes was carried out in the municipalities of Agua Prieta, Caborca, Cajeme, Hermosillo, Moctezuma, Navojoa, Sahuaripa, Santa Ana, and Ures according to the sampling method [8] and detection of
District | Area sowed (ha) | ||||
---|---|---|---|---|---|
Total | Irrigation | Raining area | Surface sampled from irrigation area 10% | Surface sampled from raining area | |
Navojoa | 3940 | 3940 | 0 | 394 | 0 |
Cajeme | 2406 | 2406 | 0 | 240.6 | 0 |
Hermosillo | 371 | 371 | 0 | 37.1 | 0 |
Ures | 115 | 115 | 0 | 11.5 | 0 |
Caborca | 2000 | 2000 | 0 | 200 | 0 |
Agua Prieta | 35 | 35 | 0 | 3.5 | 0 |
Sahuaripa | 5 | 0 | 5 | 0 | 0.5 |
Santa Ana | 30 | 30 | 0 | 3 | 0 |
Moctezuma | 1 | 1 | 0 | 0.1 | 0 |
Total | 8903 | 8898 | 5 | 889.8 | 0.5 |
Surface sowed by potato crop in different districts of Sonora state (surface sampled to detect
The results of the phytopathological diagnosis show positive
Technical of diagnosis | ||||
---|---|---|---|---|
Test SMSA | Test TZC | Test PPO | Serology technique | |
Navojoa | − | − | − | − |
Cajeme | − | − | − | − |
Hermosillo | − | + | − | − |
Ures | − | + | − | − |
Ures | − | + | − | − |
Caborca | − | − | − | − |
Agua Prieta | − | + | − | − |
Sahuaripa | − | + | − | − |
Santa Ana | − | − | − | − |
Moctezuma | − | + | − | − |
Navojoa | + | + | + | + |
Cajeme | − | − | − | − |
Hermosillo | + | + | + | + |
Ures | − | − | − | − |
Caborca | − | − | − | − |
Agua Prieta | + | + | + | + |
Sahuaripa | − | − | − | − |
Santa Ana | − | − | − | − |
Moctezuma | − | − | − | − |
Control positivo | + | + | + | + |
Control negativo (distilled water) | − | − | − | − |
Detection of
+, Test positive; −, test negative; specific media, SMSA and TZC.
In import tuber, the results were negative. Nonetheless, it is important to note that in response from abroad, there was variability of response for the municipalities of Agua Prieta, Sahuaripa, Moctezuma, Hermosillo, and Ures, as they were positive with the specific medium TZC but negative to the PPO and ELISA test [7].
Regarding the analyses carried out in seedlings, a leaf developed during the flowering stage and tubercle of fruit sampled at physiological maturity, the results indicate that for samples obtained from the municipalities of Agua Prieta, Navojoa, Hermosillo, and Caborca, they were negative when using specific media (SMSA and TZC) and serological tests, except in the PPO test that was positive for leaf and fruit (Table 3) [7].
Districts sampled | Techniques of diagnosis | |||
---|---|---|---|---|
Test SMSA | Test TZC | Test PPO | Serology technique | |
−−− | −−− | −++ | −−− | |
Seedling Leaf Fruit | −−− | +++ | +++ | −−− |
−−− | −−− | −++ | −−− | |
−−− | +++ | +++ | −−− | |
−−− | −−− | −++ | −−− | |
−−− | −−− | −++ | −−− | |
−−− | +++ | +++ | −−− | |
−−− | −−− | −−− | −−− | |
−−− | −−− | −−− | −−− | |
Control positive | + | + | + | + |
Control negative | − | − | − | − |
Detection of
+, Test positive; −, test negative; specific medium, SMSA and TZC. PPO, oxidase test; control negative = sterile distilled water.
On the other hand, when analyzing the vegetative samples from Sahuaripa, Cajeme, and Ures, the analyses showed negative results in specific medium SMSA and serological tests, the opposite occurred in culture medium TZC and PPO test. An additional test was developed to the isolated colonies of the different sampling points, resulting being Gram-negative, in a bar form, strictly aerobic, and with measurement of 0.3–0.5 × 1.0–1.5 μm. For the municipality of Moctezuma, the results indicate the negative presence of
Both oregano and thyme essential oils presented inhibition halos, such as antimicrobial activity against
The average antibacterial activity obtained from all concentrations and different amounts of oregano extract applied was 25.8 mm. The mean inhibition values found were 38.3, 20.5, and 20.2 mm in diameter, for concentrations 1:1, 1:5, and 1:10, respectively. In a similar study in which the antibacterial activity of the oils of four different varieties was evaluated, oregano on
The average antibacterial activity obtained from all concentrations and different amounts of thyme extract evaluated was 26.2 mm. The mean inhibition values found were 36.3, 28.0, and 14.1 mm in diameter, for the concentrations 1.1, 1:5, and 1.10, respectively. Our results were lower compared with [6], which obtained 50.3, 33.0, and 21.0 mm for the same concentrations to
The concentration of thyme oil that showed the greatest halo of growth inhibition of the bacterium
Inhibition of growth of
When comparing the essential oils of oregano (
In the comparison of the mean values of applied amounts (7.5, 10.15 μl) of oregano and thyme essential oils in growth inhibition halo of
Essential oil | Dilutions | Applications | Values |
---|---|---|---|
Oregano | 1:1 | 15 μl | 40.833 a |
Thyme | 1:1 | 15 μl | 40.500 a |
Oregano | 1:1 | 10 μl | 39.000 ab |
Thyme | 1:1 | 10 μl | 35.167 abc |
Oregano | 1:1 | 7.5 μl | 35.167 abc |
Thyme | 1:5 | 15 μl | 34.500 abcd |
Thyme | 1:1 | 7.5 μl | 33.500 abcde |
Thyme | 1:5 | 10 μl | 27.833 abcdef |
Thyme | 1:10 | 15 μl | 24.500 bcdef |
Oregano | 1:5 | 15 μl | 23.833 cdef |
Thyme | 1:5 | 7.5 μl | 23.000 cdef |
Oregano | 1:10 | 15 μl | 20.833 def |
Oregano | 1:10 | 7.5 μl | 20.333 ef |
Thyme | 1:10 | 10 μl | 19.833 efg |
Oregano | 1:5 | 10 μl | 19.500 efg |
Oregano | 1:5 | 7.5 μl | 18.667 fg |
Oregano | 1:10 | 10 μl | 15.000 fg |
Thyme | 1:10 | 7.5 μl | 6.000 g |
Inhibition of growth of
Values with the same letter does not exist significance (P > 0.05).
The presence of
According to the essential oils of oregano and thyme, they showed inhibitory effects on the growth of the bacterium
The essential oils of oregano and thyme showed (P < 0.05) better inhibitory effect than the antibiotics used streptomycin (10 μg) and ampicillin. Therefore, essential oils are excellent alternatives to antibiotics in the control of the bacterium
Since the outbreak of 2007 Avian Influenza, Indonesia had been starting to accumulate more knowledge and experiences related to biosafety and biosecurity. While Indonesian researchers were focusing on biorisk management of high pathogenic bioagents, students were also being exposed gradually to more sophisticated biological hazards through the utilization of synthetic biology and genetic modifications on their own researches.
\nTrainings and workshops on aforementioned issues had been organized regularly, and at the same time, High Containment Facilities as BSL3 had been built in some locations with supports from various International organizations such as BEP, WHO, and FAO. One of the projects is the establishment and evaluation of Biorisk Management in University of Indonesia [1].
\nIn 2005, WHO has established International Health Regulation (IHR) to strengthen health security, which defined as “activities required to minimize the danger and impact of acute public health events that endanger the collective health and population living across geographical regions and International boundaries.” IHR has purpose to provide swift response to global threats of spread of diseases without imposing significant disturbance to international traffic and trade [2]. One of implementations of IHR was by establishment of Global Health Security Agenda (GHSA) consortium to give participant countries such as Indonesia a capacity to prevent, detect, and respond to this spread, regardless of the root causes such as natural, deliberate, or accidental occurrences.
\nTo achieve these goals, GHSA developed concept called “Action Packages,” where Biosafety and Biosecurity are important elements. This Action Packages consist of:
\nPrevent 1: Antimicrobial Resistance
\nPrevent 2: Zoonotic Disease
\nPrevent 3: Biosafety and Biosecurity
\nPrevent 4: Immunization
\nDetect 1: National Laboratory System
\nDetects 2 and 3: Real-Time Surveillance
\nDetect 4: Reporting
\nDetect 5: Workforce Development
\nRespond 1: Emergency Operation Centers
\nRespond 2: Linking Public Health with Law and Multisectoral Rapid Response
\nRespond 3: Medical Countermeasures and Personnel Deployment Action Package [3].
\nA country achievement to GHSA Active Pack implementation is assessed voluntarily by a Joint External Evaluation (JEE). Indonesia was assessed back in 2017 by JEE with several recommendations:
to formulate necessary regulations to allow development and integration of IHR in a country multisectoral level;
to define a coordination mechanism between IHR and global security task force with relevant local ministries, agents, and institution; and
to evaluate and simplify bureaucratic structure of decision makers to enable Indonesia to act fast to national and international issues.
The report also noted the absence of National Biosafety and Biosecurity manual and the absence of select agent list and National Inventory of Biological agent [4, 5].
\nOne Health University Network and One Health Laboratory Network were established to promote Biorisk Management at university level, which was participated by Indonesian well-known university such as Gadjah Mada University, Airlangga University, and Syiah Kuala University with pilot project at their own medical and veterinary labs. Major outcomes from this project were the establishment of institutional biosafety committee, a certification program for professional biorisk management, and the development of laboratory assessment tools.
\nIn National scope, Indonesian Biosafety Association was founded in 2011 with objectives to:
build a proper capacity in biorisk management in laboratories in Indonesia (research institutions, diagnostics, industry, and hospitals);
collaborate with government and other authorities by providing technical inputs for national strategies, policies, and guidelines on biorisk management as well as advocate for a better understanding of biorisk management itself;
become an efficient, functional, and sustainable biosafety organization;
build interaction and communication between scientists and nonscientist professionals in order to support the development of biorisk in Indonesia;
provide a forum to represent the interests and needs of biorisk practitioners, as well as a source for continuous information updates; and
conduct training to ensure the implementation of safe laboratory quality and standards that are carrying out according to principles of biorisk management [6].
In addition, Biorisk Management standard had been successfully formulated under Indonesian National standard (SNI) nos. 8340:2016 and 8434:2017, which was adopted from CWA 15793 and CWA 16393, respectively [7, 8, 9, 10].
\nAt this report, we are focusing on the following areas:
select agents and risk group;
emerging and reemerging disease researches; and
researches that use emerging technologies in Indonesia.
References from National and International Biorisk Management Guidelines in health, education, veterinary, and agriculture areas in various countries were sought to provide an in-depth view on this matter. It is noted that scientific journals on biorisk management and responsible science are still limited; therefore, some sources could only be obtained from unpublished reports and webpages.
\nDefinition of select agent according to the US Centers for Disease Control and Prevention (CDC) is “biological agent and toxins determined to have the potential to pose a severe threat to public health and safety to animal and plant health or to animal or plant products.”
\nThere are 67 organisms in CDC’s list agents, which developed from initial list which contained 42 agents and toxins introduced in 1997. It included some agents that could affect both humans and animals (for example,
international commitments not to use disease as a weapon are embodied in the Geneva Protocol, which was signed in 1925 and entered into force in 1928. This protocol prohibited the usage of chemical and biological as weapons; however, it did not band the production, storage, or transfer of those materials and
the Biological and Toxin Weapons Convention (BWC), which was signed in 1972 and entered into force in 1975 as well as Chemical Weapons Convention in 1993. This closed the gap, which was not covered by Geneva Protocol previously.
Article I of the BWC states that “Each State Party to this Convention undertakes never in any circumstances to develop, produce, stockpile or otherwise acquire or retain:
Microbial or other biological agents, or toxins whatever their origin or method of production, of types and in quantities that have no justification for prophylactic, protective or other peaceful purposes;
Weapons, equipment or means of delivery designed to use such agents or toxins for hostile purposes or in armed conflict.”
The BWC does not prohibit research on defenses against biological weapons where a number of countries, including the USA and its major allies, have continued to do. Indonesia signed the BWC in 1972 and signed the ratification in 1992. Currently, there are 183 countries that have ratified BWC [11].
\nIn developed countries such as the USA, Europe, Canada, China, and Singapore, select agents have been defined well, but most of the developing countries including Indonesia do not have it yet [12, 13, 14, 15, 16]. Resources of biological agents in developing countries are very significant; thus with the presence of various biological agents and toxins, it can cause a real threat. Some countries can refer to the biological agent list of the US CDC, but this is not a universal list because it may contain some preferences to the US national interest. Developing countries should evaluate characteristics of each biological agent and threats faced, existing biodefense capabilities based on its specific regional conditions. The CDCs of developing country play an important role in this process and cooperate with other related departments and organizations [17]. Although some US organizations had facilitated discussion with relevant Indonesian institution and initiate list of select agents but the list could not be defined yet. Indonesian Ministry of Agriculture has “red microorganisms,” where the usage and distribution must be limited to certain laboratories; however, it is not a standard practice for others. Nowadays, there is no select agent list in Indonesia. Controlling the work of using these pathogens and high pathogenic organisms has been performed by each institution individually. Most of laboratories do not have the awareness about the importance to limit the usage of select agents. Furthermore, Indonesia also does not have risk group category criterion. WHO or CDC criterions were followed, which sometimes may not be suitable with local condition (Table 1).
\nGroup | \nWHO [18] | \nNIH [19] | \nEU [13] | \nChina [20] | \n
---|---|---|---|---|
1 | \nWell-characterized agents are likely not cause human or animal disease. | \nAgents are not associated with disease in healthy adult humans. | \nAgents are unlikely cause human disease. | \nAgents do not cause human or animal disease under normal circumstances. | \n
2 | \nAgents cause human or animal disease but unlikely to be a serious hazard to laboratory workers, the community, the livestock, or the environment. Effective treatment and preventive measure are available. | \nAgents are associated with human disease, which is rarely serious. Effective treatment and preventive measure are often available. | \nAgents can cause human disease and might be a hazard to workers. It is unlikely to spread to the community. Effective prophylaxis or treatment is usually available. | \nAgents cause human or animal disease under normal circumstances but do not pose a serious hazard to people, animals, or the environment, and the risk of transmission is limited. Laboratory-associated infection rarely causes serious illness with effective treatment and prevention. | \n
3 | \nAgents usually cause serious human or animal disease but do not spread from one individual to another. Effective treatment and preventive measures are available. | \nAgents are associated with serious or lethal human disease. Preventive or therapeutic interventions may be available. Risk of spreading to individual is high but to community is low. | \nAgents can cause severe disease and pose a serious hazard to workers. It may spread to community, but effective prophylaxis or treatment is usually available. | \nAgents can cause serious human or animal disease. It is relatively easy to spread between people, animals and people, among animals, directly or indirectly. | \n
4 | \nAgents usually cause serious human or animal disease. They can be readily transmitted from one individual to another, directly or indirectly. Effective treatment and preventive measures are not usually available. | \nAgents likely cause serious or lethal human disease. Preventive or therapeutic interventions are not usually available. Risk of spreading to individual and to community is high. | \nAgents cause severe human disease and pose a serious hazard to workers. Risk of spreading to community is high. Effective prophylaxis or treatment is usually not available. | \nAgents can cause very serious disease in humans and animals, including biological agents that have not been found in China. | \n
Comparison of biological agent category criterion based on biosafety.
Other important subjects are emerging and reemerging infectious diseases, which could pose a major threat to global public health. On the other hand, these subjects also attract many scientists to obtain good publications and funding. Despite of all efforts in biorisk management, unfortunately the handling of pathogenic viruses remains a likely source of infection, and mortality, among laboratory workers [21].
\nAccidental infections of workers in hospitals or research laboratories are an emerging threat mainly due to the increasing amount of research with it being carried out involving the Risk Group 3 or 4 [22]. Infections due to the blood borne emerging viruses such as hepatitis C and HIV are the commonest diagnosed viral infections [23]. Laboratory-acquired infection by other emerging viruses such as SARS, Marburg, dengue, vaccinia, Crimean-Congo hemorrhagic fever, Western equine encephalitis, West Nile virus, and Zika has also been reported [24, 25, 26, 27, 28, 29, 30].
\nA strict biorisk assessment should be applied prior to experiments. A sufficient mitigation should be assessed by biosafety officer and Biosafety committee. Unfortunately, only some institutions in Indonesia have Institutional Biosafety Committee and do not have National Biosafety Committee yet. Currently, assessment was performed together, and decision was made based on available mitigation in the laboratory (Figure 1).
\nHepatitis and Ebola virus.
Rapid progress of technologies does not only exclusively belong to developed countries nowadays but also spread out to developing countries including Indonesia. Life sciences such a molecular biology, bioengineering, genetic engineering, bioinformatics, and synthetic biology could not be more important nowadays since it plays a pivot role in developing translational medicines and biotechnologies. There are many Indonesian universities and emerging translational medical centers that had declared themselves as research centers focusing on to bring science from bench to bedside. Also, many biotechnology centers provide sources for livestock, pharmacy, cosmetics, industry, and many more. They always try to bring experiments to applications.
\nOn the other hand, the development in life science is no longer obtained exclusively through formal educations but could also be acquired from powerful informal sources such as Internet and social network. Without a proper guidance, biosafety, biosecurity, and dual use issues can become a major risk in human life itself. It could be said that biosafety focuses on procedures and techniques to prevent an accidental or unintentional release of bioagents, and biosecurity focuses more on accountability measurements and procedures to protect bioagents from unauthorized access, misused, thievery act of an intentional release [9, 31, 32]. Dual use according to the US Government is “a life sciences research that, based on current understanding, can be reasonably anticipated to provide knowledge, information, products, or technologies that could be directly misapplied to pose a significant threat with broad potential consequences to public health and safety, agricultural crops and other plants, animals, the environment, materiel, or national security.” The US Government’s oversight of DURC is aimed at preserving the benefits of life science research while minimizing the risk of misuse of the knowledge, information, products, or technologies provided by such research. Whereas WHO’s definition is “life sciences research that is intended for benefit, but which might easily be misapplied to do harm.”
\nDuring this time, we also met with students whom very interested in participating on International Genetically Engineered Machine (IGEM), where Indonesia is an active participant since 2013. On one of IGEM project on TBC diagnostic system called “Blue Ivy Project,” it brought us to important realization regarding dealing with the amplification of risk in growing cultures and biofilm where rigorous SOPs during works were required to satisfy its biosafety and biosecurity aspect of this project (Figure 2) [33].
\nIGEM project in University of Indonesia.
Challenges in biosafety and biosecurity became more complicated year by year such as “BaContraception Project” to design contraception by using
In the next project, the Hi Vax project, we knew that Indonesia got attention from the IGEM board as we got information from FBI during its workshop in Jakarta. They would like to make us aware that technologies are progressing fast in Indonesia and urge a control going along it to not cause harm and threats. Hi Vax project is a project to make HIV DNA Vaccine. Basically, they are capable of making the HIV proteins with this system. It is why FBI emphasized this issue to all stakeholders in Indonesia to have concerns about the science progress in Indonesia and think about the risk particularly dual use research of concerns along the progress of technologies. In addition, in the recent IGEM project which working with the synthetic toxin of Diphtheria, team started to establish carefully the risk assessment, so they are capable of analyzing the possibility of dual use research and communicating it to IGEM’s Biosafety and Biosecurity Board [35].
\nBased on our experiment, we realize the urgent of need of National Biosafety Committee or at least Institutional Biosafety Review Board. But, how many lectures or researchers have concern about this? Whereas many new emerging technologies are coming with fast progressing to arrive in the border of ethical problems and of course, the biosafety, biosecurity, and dual use research of concern. Recombinant protein, Genetically Modified Organisms, induced-Pluripotent Stem Cell (iPS), Gain of Function (GoF), and CRISPR (clusters of regularly interspaced short palindromic repeats) are now coming and become a common methods at life science laboratories. In addition, DIY-bio (Do it Your self Biology) is omnipresent at the same time of the emerging of biotechnology program and biotechnology start up. They must be equipped with a sufficient Biorisk Management. Recombinant protein is a technology to produce protein made based on DNA recombinant that has been cloned in system that supports expression of the gene. Modification of gene by using recombinant technology leads to the expression of a mutant protein, over expression or suppress expression. Gain of Function (GoF) is a research that involves experimentation that aims or is expected to (and/or, perhaps, actually does) increase the transmissibility and/or virulence of pathogens. The aim of GOF research is to improve understanding of disease causing agents, their interaction with human hosts, and/or their potential to cause pandemics. GOF research (GOFR) can pose risks regarding biosecurity and biosafety. Nowadays, new technologies that become a star are CRISPR and iPS. CRISPR or CRISPR Cas 9 system allows genetic material to be added, removed, or altered at particular locations in the genome, whereas iPS allows whatever cells in the body to be reprogram into stem cells. These future technologies are expected to resolve many problems in disease therapies, and at the same time, we have to minimize the side effects of these technologies.
\nThe advance of biotechnology benefits for life science research. It resulted in important biomedical products and resolved many health problems. However, advances in technology and research can unintentionally lead to techniques and/or findings that:
increase virulence, transmission, or host range of a pathogen;
confer antibiotic resistance so as to decrease currently effective treatments;
enable evasion of currently existing diagnostic or detection mechanisms; and
demonstrate weaponization of a pathogen.
WHO created guideline Responsible life science research for global health security for promoting excellent, safe, secure and responsible life science research. It consists of three pillars supporting public health:
research excellence;
ethics; and
biosafety and laboratory biosecurity.
This guideline shows that the best protection against the possibility of accidents and deliberate misuse of life science can be attained by promoting culture of scientific integrity and excellence and distinguished by openness, honesty, accountability, and responsibility (Figure 3).
\nBiorisk management framework for responsible life science research.
Research excellence means encouraging quality of life science activities that serve as the basis of development of new treatments and therapeutics. It supports health research system and promotes public health surveillance and response activities. These all elements are essential to protect and improve health and well-being of all people. To attain them, countries or institutions are requested to:
support capacity development for research and
use existing tools and frameworks, which are health research systems (HRS), the WHO strategy on research for health, and the International Health Regulations (IHR) as these can provide useful tools for contributing to responsible life science research.
Ethics are promoting responsible and good research practices. It provides tools and practices to scientists and institutions that allow them to discuss, analyze, and resolve dilemmas they may face in research including problem related to the possibility of accidents or misuse of the life sciences. Countries and institutions are requested to:
use current ethical platforms, if appropriate;
promote ethics education and training for students and professionals;
encourage discussion and reflection on research practices;
hold institutions and researchers to account and ensure they are aware of their responsibilities; and
ensure institutions and researchers are aware of existing and new legislation, regulations not only at the country but also at the regional and international levels.
Biosafety and biosecurity are the implementation and strengthening of measures and procedures to:
minimize the risk of worker exposure to pathogens and infections;
protect the environment and the community; and
protect, control, and account for valuable biological materials (VBMs).
All measures are applied in the laboratory or institution in order to prevent accidental and deliberate release of pathogens and valuable biological materials. They are aimed to ensure a safe and secure laboratory environment. Countries and institutions are requested to:
conduct biosafety and laboratory biosecurity risk assessments and prepare mitigations to reduce the risk;
implement a laboratory biorisk management system;
explore the use of existing biorisk management structures (e.g., laboratory biorisk management adviser and the biosafety committee) to address issues related to the risks posed by life science research; and
set performance objectives and work on continuous improvement [36].
The progress of biotechnologies in developing countries especially in Indonesia does not walk in parallel with the raising of awareness for more responsible science, which gives an alert for future development on life science research itself. Thus, a task to raise awareness for more responsible science should be done in all sectors particularly in universities and research centers. In addition, responsible science must reach young scientists and spread at all universities in Indonesia.
\nAlthough Indonesia had established national standards for biorisk management, yet they are still on voluntary basis and not implemented in all related institutions. Thus, establishment of more detail guidelines and compulsory regulations on biorisk management should be accelerated and performed by all stakeholders.
\nUniversity medical research center is one of a front line to face emerging technologies and emerging diseases. It is a place that could bring a silver lining in developing health security, but at the same time, it could pose a concern on dual use research application.
\nMedical research center is a good place to start applying more responsible science and nurturing the next generation of scientists whom have more awareness on biorisk issues. However, it is still long way to go at least for Indonesia to establish the biosafety committee at institutions as well as at national level. A need to define National Select Agents and Risk group microorganisms is also noted to provide guideline and better support for biorisk management itself.
\nRegardless all limitations above, efforts to raise awareness in young scientists should continue not only by established organizations such as Indonesian Biorisk Association and One Health Laboratories Network but also by full supports from other relevant stakeholders in order to motivate them to create a better research environment.
\nWe thank Indonesian Biorisk Association and One Health Laboratories Networking for the additional information. We would also like to thank Hendri Bundrawan for manuscript preparation, English correction, and editing.
\nIntechOpen implements a robust policy to minimize and deal with instances of fraud or misconduct. As part of our general commitment to transparency and openness, and in order to maintain high scientific standards, we have a well-defined editorial policy regarding Retractions and Corrections.
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\\n\\n1. RETRACTIONS
\\n\\nA Retraction of a Chapter will be issued by the Academic Editor, either following an Author’s request to do so or when there is a 3rd party report of scientific misconduct. Upon receipt of a report by a 3rd party, the Academic Editor will investigate any allegations of scientific misconduct, working in cooperation with the Author(s) and their institution(s).
\\n\\nA formal Retraction will be issued when there is clear and conclusive evidence of any of the following:
\\n\\nPublishing of a Retraction Notice will adhere to the following guidelines:
\\n\\n1.2. REMOVALS AND CANCELLATIONS
\\n\\n2. STATEMENTS OF CONCERN
\\n\\nA Statement of Concern detailing alleged misconduct will be issued by the Academic Editor or publisher following a 3rd party report of scientific misconduct when:
\\n\\nIntechOpen believes that the number of occasions on which a Statement of Concern is issued will be very few in number. In all cases when such a decision has been taken by the Academic Editor the decision will be reviewed by another editor to whom the author can make representations.
\\n\\n3. CORRECTIONS
\\n\\nA Correction will be issued by the Academic Editor when:
\\n\\n3.1. ERRATUM
\\n\\nAn Erratum will be issued by the Academic Editor when it is determined that a mistake in a Chapter originates from the production process handled by the publisher.
\\n\\nA published Erratum will adhere to the Retraction Notice publishing guidelines outlined above.
\\n\\n3.2. CORRIGENDUM
\\n\\nA Corrigendum will be issued by the Academic Editor when it is determined that a mistake in a Chapter is a result of an Author’s miscalculation or oversight. A published Corrigendum will adhere to the Retraction Notice publishing guidelines outlined above.
\\n\\n4. FINAL REMARKS
\\n\\nIntechOpen wishes to emphasize that the final decision on whether a Retraction, Statement of Concern, or a Correction will be issued rests with the Academic Editor. The publisher is obliged to act upon any reports of scientific misconduct in its publications and to make a reasonable effort to facilitate any subsequent investigation of such claims.
\\n\\nIn the case of Retraction or removal of the Work, the publisher will be under no obligation to refund the APC.
\\n\\nThe general principles set out above apply to Retractions and Corrections issued in all IntechOpen publications.
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\\n\\nPolicy last updated: 2017-09-11
\\n"}]'},components:[{type:"htmlEditorComponent",content:'IntechOpen’s Retraction and Correction Policy has been developed in accordance with the Committee on Publication Ethics (COPE) publication guidelines relating to scientific misconduct and research ethics:
\n\n1. RETRACTIONS
\n\nA Retraction of a Chapter will be issued by the Academic Editor, either following an Author’s request to do so or when there is a 3rd party report of scientific misconduct. Upon receipt of a report by a 3rd party, the Academic Editor will investigate any allegations of scientific misconduct, working in cooperation with the Author(s) and their institution(s).
\n\nA formal Retraction will be issued when there is clear and conclusive evidence of any of the following:
\n\nPublishing of a Retraction Notice will adhere to the following guidelines:
\n\n1.2. REMOVALS AND CANCELLATIONS
\n\n2. STATEMENTS OF CONCERN
\n\nA Statement of Concern detailing alleged misconduct will be issued by the Academic Editor or publisher following a 3rd party report of scientific misconduct when:
\n\nIntechOpen believes that the number of occasions on which a Statement of Concern is issued will be very few in number. In all cases when such a decision has been taken by the Academic Editor the decision will be reviewed by another editor to whom the author can make representations.
\n\n3. CORRECTIONS
\n\nA Correction will be issued by the Academic Editor when:
\n\n3.1. ERRATUM
\n\nAn Erratum will be issued by the Academic Editor when it is determined that a mistake in a Chapter originates from the production process handled by the publisher.
\n\nA published Erratum will adhere to the Retraction Notice publishing guidelines outlined above.
\n\n3.2. CORRIGENDUM
\n\nA Corrigendum will be issued by the Academic Editor when it is determined that a mistake in a Chapter is a result of an Author’s miscalculation or oversight. A published Corrigendum will adhere to the Retraction Notice publishing guidelines outlined above.
\n\n4. FINAL REMARKS
\n\nIntechOpen wishes to emphasize that the final decision on whether a Retraction, Statement of Concern, or a Correction will be issued rests with the Academic Editor. The publisher is obliged to act upon any reports of scientific misconduct in its publications and to make a reasonable effort to facilitate any subsequent investigation of such claims.
\n\nIn the case of Retraction or removal of the Work, the publisher will be under no obligation to refund the APC.
\n\nThe general principles set out above apply to Retractions and Corrections issued in all IntechOpen publications.
\n\nAny suggestions or comments on this Policy are welcome and may be sent to permissions@intechopen.com.
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The most important one is that orthodontic appliances especially the brackets and the ligation mode create new retention areas in addition to blocking plaque-removing shear forces arising from fluid flow and masticatory loads with a resultant undesired effect of accumulation of dental plaque. Increased amount of dental plaque containing cariogenic bacteria is the main etiologic factor in decalcification of enamel during orthodontic treatment. This demineralization of the tooth surfaces results in appearance of white spots or even caries. However, in the literature, there are conflicting results in the relationship between orthodontic treatment and development of dental caries. Many preventive methods such as topical fluoride application, using bonding materials releasing fluoride, using mouth rinse with sodium fluoride, applying chlorhexidine, and so on were defined. The general comment of the authors is that supplying an adequate oral hygiene has the main role in prevention of demineralization-caries during orthodontic treatment. In the light of the previous studies’ results, it can be concluded that professional application like a varnish can be provided for patients who have high caries incidence.",book:{id:"6668",slug:"dental-caries-diagnosis-prevention-and-management",title:"Dental Caries",fullTitle:"Dental Caries - Diagnosis, Prevention and Management"},signatures:"Gamze Metin-Gürsoy and Fatma Deniz Uzuner",authors:[{id:"172009",title:"Dr.",name:"Fatma Deniz",middleName:null,surname:"Uzuner",slug:"fatma-deniz-uzuner",fullName:"Fatma Deniz Uzuner"},{id:"235169",title:"Dr.",name:"Gamze",middleName:null,surname:"Metin Gürsoy",slug:"gamze-metin-gursoy",fullName:"Gamze Metin Gürsoy"}]}],onlineFirstChaptersFilter:{topicId:"995",limit:6,offset:0},onlineFirstChaptersCollection:[],onlineFirstChaptersTotal:0},preDownload:{success:null,errors:{}},subscriptionForm:{success:null,errors:{}},aboutIntechopen:{},privacyPolicy:{},peerReviewing:{},howOpenAccessPublishingWithIntechopenWorks:{},sponsorshipBooks:{sponsorshipBooks:[],offset:8,limit:8,total:0},allSeries:{pteSeriesList:[{id:"14",title:"Artificial Intelligence",numberOfPublishedBooks:9,numberOfPublishedChapters:87,numberOfOpenTopics:6,numberOfUpcomingTopics:0,issn:"2633-1403",doi:"10.5772/intechopen.79920",isOpenForSubmission:!0},{id:"7",title:"Biomedical Engineering",numberOfPublishedBooks:12,numberOfPublishedChapters:99,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2631-5343",doi:"10.5772/intechopen.71985",isOpenForSubmission:!0}],lsSeriesList:[{id:"11",title:"Biochemistry",numberOfPublishedBooks:27,numberOfPublishedChapters:290,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2632-0983",doi:"10.5772/intechopen.72877",isOpenForSubmission:!0},{id:"25",title:"Environmental Sciences",numberOfPublishedBooks:1,numberOfPublishedChapters:9,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2754-6713",doi:"10.5772/intechopen.100362",isOpenForSubmission:!0},{id:"10",title:"Physiology",numberOfPublishedBooks:11,numberOfPublishedChapters:139,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2631-8261",doi:"10.5772/intechopen.72796",isOpenForSubmission:!0}],hsSeriesList:[{id:"3",title:"Dentistry",numberOfPublishedBooks:8,numberOfPublishedChapters:129,numberOfOpenTopics:0,numberOfUpcomingTopics:2,issn:"2631-6218",doi:"10.5772/intechopen.71199",isOpenForSubmission:!1},{id:"6",title:"Infectious Diseases",numberOfPublishedBooks:13,numberOfPublishedChapters:108,numberOfOpenTopics:3,numberOfUpcomingTopics:1,issn:"2631-6188",doi:"10.5772/intechopen.71852",isOpenForSubmission:!0},{id:"13",title:"Veterinary Medicine and Science",numberOfPublishedBooks:11,numberOfPublishedChapters:104,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2632-0517",doi:"10.5772/intechopen.73681",isOpenForSubmission:!0}],sshSeriesList:[{id:"22",title:"Business, Management and Economics",numberOfPublishedBooks:1,numberOfPublishedChapters:12,numberOfOpenTopics:2,numberOfUpcomingTopics:1,issn:"2753-894X",doi:"10.5772/intechopen.100359",isOpenForSubmission:!0},{id:"23",title:"Education and Human Development",numberOfPublishedBooks:0,numberOfPublishedChapters:1,numberOfOpenTopics:2,numberOfUpcomingTopics:0,issn:null,doi:"10.5772/intechopen.100360",isOpenForSubmission:!1},{id:"24",title:"Sustainable Development",numberOfPublishedBooks:0,numberOfPublishedChapters:12,numberOfOpenTopics:5,numberOfUpcomingTopics:0,issn:null,doi:"10.5772/intechopen.100361",isOpenForSubmission:!0}],testimonialsList:[{id:"6",text:"It is great to work with the IntechOpen to produce a worthwhile collection of research that also becomes a great educational resource and guide for future research endeavors.",author:{id:"259298",name:"Edward",surname:"Narayan",institutionString:null,profilePictureURL:"https://mts.intechopen.com/storage/users/259298/images/system/259298.jpeg",slug:"edward-narayan",institution:{id:"3",name:"University of Queensland",country:{id:null,name:"Australia"}}}},{id:"13",text:"The collaboration with and support of the technical staff of IntechOpen is fantastic. 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He obtained a Master’s degree in Public Health and PhD in Public Health and Epidemiology. He has a background in Clinical Medicine and has taken courses at higher diploma levels in public health from University of Transkei, Republic of South Africa, and African Medical and Research Foundation (AMREF) in Nairobi, Kenya. Dr. Kasenga worked in different places in and outside Malawi, and has held various positions, such as Licensed Medical Officer, HIV/AIDS Programme Officer, HIV/AIDS resource person in the International Department of Diakonhjemet College, Oslo, Norway. He also managed an Integrated HIV/AIDS Prevention programme for over 5 years. He is currently working as a Director for the Health Ministries Department of Malawi Union of the Seventh Day Adventist Church. Dr. Kasenga has published over 5 articles on HIV/AIDS issues focusing on Prevention of Mother to Child Transmission of HIV (PMTCT), including a book chapter on HIV testing counseling (currently in press). 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His research interest focuses on computational chemistry and molecular modeling of diverse systems of pharmacological, food, and alternative energy interests by resorting to DFT and Conceptual DFT. He has authored a coauthored more than 255 peer-reviewed papers, 32 book chapters, and 2 edited books. He has delivered speeches at many international and domestic conferences. He serves as a reviewer for more than eighty international journals, books, and research proposals as well as an editor for special issues of renowned scientific journals.",institutionString:"Centro de Investigación en Materiales Avanzados",institution:{name:"Centro de Investigación en Materiales Avanzados",country:{name:"Mexico"}}},{id:"76477",title:"Prof.",name:"Mirza",middleName:null,surname:"Hasanuzzaman",slug:"mirza-hasanuzzaman",fullName:"Mirza Hasanuzzaman",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/76477/images/system/76477.png",biography:"Dr. Mirza Hasanuzzaman is a Professor of Agronomy at Sher-e-Bangla Agricultural University, Bangladesh. He received his Ph.D. in Plant Stress Physiology and Antioxidant Metabolism from Ehime University, Japan, with a scholarship from the Japanese Government (MEXT). Later, he completed his postdoctoral research at the Center of Molecular Biosciences, University of the Ryukyus, Japan, as a recipient of the Japan Society for the Promotion of Science (JSPS) postdoctoral fellowship. He was also the recipient of the Australian Government Endeavour Research Fellowship for postdoctoral research as an adjunct senior researcher at the University of Tasmania, Australia. Dr. Hasanuzzaman’s current work is focused on the physiological and molecular mechanisms of environmental stress tolerance. Dr. Hasanuzzaman has published more than 150 articles in peer-reviewed journals. He has edited ten books and written more than forty book chapters on important aspects of plant physiology, plant stress tolerance, and crop production. According to Scopus, Dr. Hasanuzzaman’s publications have received more than 10,500 citations with an h-index of 53. He has been named a Highly Cited Researcher by Clarivate. He is an editor and reviewer for more than fifty peer-reviewed international journals and was a recipient of the “Publons Peer Review Award” in 2017, 2018, and 2019. He has been honored by different authorities for his outstanding performance in various fields like research and education, and he has received the World Academy of Science Young Scientist Award (2014) and the University Grants Commission (UGC) Award 2018. He is a fellow of the Bangladesh Academy of Sciences (BAS) and the Royal Society of Biology.",institutionString:"Sher-e-Bangla Agricultural University",institution:{name:"Sher-e-Bangla Agricultural University",country:{name:"Bangladesh"}}},{id:"187859",title:"Prof.",name:"Kusal",middleName:"K.",surname:"Das",slug:"kusal-das",fullName:"Kusal Das",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bSBDeQAO/Profile_Picture_1623411145568",biography:"Kusal K. Das is a Distinguished Chair Professor of Physiology, Shri B. M. Patil Medical College and Director, Centre for Advanced Medical Research (CAMR), BLDE (Deemed to be University), Vijayapur, Karnataka, India. Dr. Das did his M.S. and Ph.D. in Human Physiology from the University of Calcutta, Kolkata. His area of research is focused on understanding of molecular mechanisms of heavy metal activated low oxygen sensing pathways in vascular pathophysiology. He has invented a new method of estimation of serum vitamin E. His expertise in critical experimental protocols on vascular functions in experimental animals was well documented by his quality of publications. He was a Visiting Professor of Medicine at University of Leeds, United Kingdom (2014-2016) and Tulane University, New Orleans, USA (2017). For his immense contribution in medical research Ministry of Science and Technology, Government of India conferred him 'G.P. Chatterjee Memorial Research Prize-2019” and he is also the recipient of 'Dr.Raja Ramanna State Scientist Award 2015” by Government of Karnataka. He is a Fellow of the Royal Society of Biology (FRSB), London and Honorary Fellow of Karnataka Science and Technology Academy, Department of Science and Technology, Government of Karnataka.",institutionString:"BLDE (Deemed to be University), India",institution:null},{id:"243660",title:"Dr.",name:"Mallanagouda Shivanagouda",middleName:null,surname:"Biradar",slug:"mallanagouda-shivanagouda-biradar",fullName:"Mallanagouda Shivanagouda Biradar",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/243660/images/system/243660.jpeg",biography:"M. S. Biradar is Vice Chancellor and Professor of Medicine of\nBLDE (Deemed to be University), Vijayapura, Karnataka, India.\nHe obtained his MD with a gold medal in General Medicine and\nhas devoted himself to medical teaching, research, and administrations. He has also immensely contributed to medical research\non vascular medicine, which is reflected by his numerous publications including books and book chapters. Professor Biradar was\nalso Visiting Professor at Tulane University School of Medicine, New Orleans, USA.",institutionString:"BLDE (Deemed to be University)",institution:{name:"BLDE University",country:{name:"India"}}},{id:"289796",title:"Dr.",name:"Swastika",middleName:null,surname:"Das",slug:"swastika-das",fullName:"Swastika Das",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/289796/images/system/289796.jpeg",biography:"Swastika N. Das is Professor of Chemistry at the V. P. Dr. P. G.\nHalakatti College of Engineering and Technology, BLDE (Deemed\nto be University), Vijayapura, Karnataka, India. She obtained an\nMSc, MPhil, and PhD in Chemistry from Sambalpur University,\nOdisha, India. Her areas of research interest are medicinal chemistry, chemical kinetics, and free radical chemistry. She is a member\nof the investigators who invented a new modified method of estimation of serum vitamin E. She has authored numerous publications including book\nchapters and is a mentor of doctoral curriculum at her university.",institutionString:"BLDEA’s V.P.Dr.P.G.Halakatti College of Engineering & Technology",institution:{name:"BLDE University",country:{name:"India"}}},{id:"248459",title:"Dr.",name:"Akikazu",middleName:null,surname:"Takada",slug:"akikazu-takada",fullName:"Akikazu Takada",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/248459/images/system/248459.png",biography:"Akikazu Takada was born in Japan, 1935. After graduation from\nKeio University School of Medicine and finishing his post-graduate studies, he worked at Roswell Park Memorial Institute NY,\nUSA. He then took a professorship at Hamamatsu University\nSchool of Medicine. In thrombosis studies, he found the SK\npotentiator that enhances plasminogen activation by streptokinase. He is very much interested in simultaneous measurements\nof fatty acids, amino acids, and tryptophan degradation products. By using fatty\nacid analyses, he indicated that plasma levels of trans-fatty acids of old men were\nfar higher in the US than Japanese men. . He also showed that eicosapentaenoic acid\n(EPA) and docosahexaenoic acid (DHA) levels are higher, and arachidonic acid\nlevels are lower in Japanese than US people. By using simultaneous LC/MS analyses\nof plasma levels of tryptophan metabolites, he recently found that plasma levels of\nserotonin, kynurenine, or 5-HIAA were higher in patients of mono- and bipolar\ndepression, which are significantly different from observations reported before. In\nview of recent reports that plasma tryptophan metabolites are mainly produced by\nmicrobiota. He is now working on the relationships between microbiota and depression or autism.",institutionString:"Hamamatsu University School of Medicine",institution:{name:"Hamamatsu University School of Medicine",country:{name:"Japan"}}},{id:"137240",title:"Prof.",name:"Mohammed",middleName:null,surname:"Khalid",slug:"mohammed-khalid",fullName:"Mohammed Khalid",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/137240/images/system/137240.png",biography:"Mohammed Khalid received his B.S. degree in chemistry in 2000 and Ph.D. degree in physical chemistry in 2007 from the University of Khartoum, Sudan. He moved to School of Chemistry, Faculty of Science, University of Sydney, Australia in 2009 and joined Dr. Ron Clarke as a postdoctoral fellow where he worked on the interaction of ATP with the phosphoenzyme of the Na+/K+-ATPase and dual mechanisms of allosteric acceleration of the Na+/K+-ATPase by ATP; then he went back to Department of Chemistry, University of Khartoum as an assistant professor, and in 2014 he was promoted as an associate professor. In 2011, he joined the staff of Department of Chemistry at Taif University, Saudi Arabia, where he is currently an assistant professor. His research interests include the following: P-Type ATPase enzyme kinetics and mechanisms, kinetics and mechanisms of redox reactions, autocatalytic reactions, computational enzyme kinetics, allosteric acceleration of P-type ATPases by ATP, exploring of allosteric sites of ATPases, and interaction of ATP with ATPases located in cell membranes.",institutionString:"Taif University",institution:{name:"Taif University",country:{name:"Saudi Arabia"}}},{id:"63810",title:"Prof.",name:"Jorge",middleName:null,surname:"Morales-Montor",slug:"jorge-morales-montor",fullName:"Jorge Morales-Montor",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/63810/images/system/63810.png",biography:"Dr. Jorge Morales-Montor was recognized with the Lola and Igo Flisser PUIS Award for best graduate thesis at the national level in the field of parasitology. He received a fellowship from the Fogarty Foundation to perform postdoctoral research stay at the University of Georgia. He has 153 journal articles to his credit. He has also edited several books and published more than fifty-five book chapters. He is a member of the Mexican Academy of Sciences, Latin American Academy of Sciences, and the National Academy of Medicine. He has received more than thirty-five awards and has supervised numerous bachelor’s, master’s, and Ph.D. students. Dr. Morales-Montor is the past president of the Mexican Society of Parasitology.",institutionString:"National Autonomous University of Mexico",institution:{name:"National Autonomous University of Mexico",country:{name:"Mexico"}}},{id:"217215",title:"Dr.",name:"Palash",middleName:null,surname:"Mandal",slug:"palash-mandal",fullName:"Palash Mandal",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/217215/images/system/217215.jpeg",biography:null,institutionString:"Charusat University",institution:null},{id:"49739",title:"Dr.",name:"Leszek",middleName:null,surname:"Szablewski",slug:"leszek-szablewski",fullName:"Leszek Szablewski",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/49739/images/system/49739.jpg",biography:"Leszek Szablewski is a professor of medical sciences. He received his M.S. in the Faculty of Biology from the University of Warsaw and his PhD degree from the Institute of Experimental Biology Polish Academy of Sciences. He habilitated in the Medical University of Warsaw, and he obtained his degree of Professor from the President of Poland. Professor Szablewski is the Head of Chair and Department of General Biology and Parasitology, Medical University of Warsaw. Professor Szablewski has published over 80 peer-reviewed papers in journals such as Journal of Alzheimer’s Disease, Biochim. Biophys. Acta Reviews of Cancer, Biol. Chem., J. Biomed. Sci., and Diabetes/Metabol. Res. Rev, Endocrine. He is the author of two books and four book chapters. He has edited four books, written 15 scripts for students, is the ad hoc reviewer of over 30 peer-reviewed journals, and editorial member of peer-reviewed journals. Prof. Szablewski’s research focuses on cell physiology, genetics, and pathophysiology. He works on the damage caused by lack of glucose homeostasis and changes in the expression and/or function of glucose transporters due to various diseases. He has given lectures, seminars, and exercises for students at the Medical University.",institutionString:"Medical University of Warsaw",institution:{name:"Medical University of Warsaw",country:{name:"Poland"}}},{id:"173123",title:"Dr.",name:"Maitham",middleName:null,surname:"Khajah",slug:"maitham-khajah",fullName:"Maitham Khajah",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/173123/images/system/173123.jpeg",biography:"Dr. Maitham A. Khajah received his degree in Pharmacy from Faculty of Pharmacy, Kuwait University, in 2003 and obtained his PhD degree in December 2009 from the University of Calgary, Canada (Gastrointestinal Science and Immunology). Since January 2010 he has been assistant professor in Kuwait University, Faculty of Pharmacy, Department of Pharmacology and Therapeutics. His research interest are molecular targets for the treatment of inflammatory bowel disease (IBD) and the mechanisms responsible for immune cell chemotaxis. He cosupervised many students for the MSc Molecular Biology Program, College of Graduate Studies, Kuwait University. Ever since joining Kuwait University in 2010, he got various grants as PI and Co-I. He was awarded the Best Young Researcher Award by Kuwait University, Research Sector, for the Year 2013–2014. He was a member in the organizing committee for three conferences organized by Kuwait University, Faculty of Pharmacy, as cochair and a member in the scientific committee (the 3rd, 4th, and 5th Kuwait International Pharmacy Conference).",institutionString:"Kuwait University",institution:{name:"Kuwait University",country:{name:"Kuwait"}}},{id:"195136",title:"Dr.",name:"Aya",middleName:null,surname:"Adel",slug:"aya-adel",fullName:"Aya Adel",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/195136/images/system/195136.jpg",biography:"Dr. Adel works as an Assistant Lecturer in the unit of Phoniatrics, Department of Otolaryngology, Ain Shams University in Cairo, Egypt. Dr. Adel is especially interested in joint attention and its impairment in autism spectrum disorder",institutionString:"Ain Shams University",institution:{name:"Ain Shams University",country:{name:"Egypt"}}},{id:"94911",title:"Dr.",name:"Boulenouar",middleName:null,surname:"Mesraoua",slug:"boulenouar-mesraoua",fullName:"Boulenouar Mesraoua",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/94911/images/system/94911.png",biography:"Dr Boulenouar Mesraoua is the Associate Professor of Clinical Neurology at Weill Cornell Medical College-Qatar and a Consultant Neurologist at Hamad Medical Corporation at the Neuroscience Department; He graduated as a Medical Doctor from the University of Oran, Algeria; he then moved to Belgium, the City of Liege, for a Residency in Internal Medicine and Neurology at Liege University; after getting the Belgian Board of Neurology (with high marks), he went to the National Hospital for Nervous Diseases, Queen Square, London, United Kingdom for a fellowship in Clinical Neurophysiology, under Pr Willison ; Dr Mesraoua had also further training in Epilepsy and Continuous EEG Monitoring for two years (from 2001-2003) in the Neurophysiology department of Zurich University, Switzerland, under late Pr Hans Gregor Wieser ,an internationally known epileptologist expert. \n\nDr B. Mesraoua is the Director of the Neurology Fellowship Program at the Neurology Section and an active member of the newly created Comprehensive Epilepsy Program at Hamad General Hospital, Doha, Qatar; he is also Assistant Director of the Residency Program at the Qatar Medical School. \nDr B. Mesraoua's main interests are Epilepsy, Multiple Sclerosis, and Clinical Neurology; He is the Chairman and the Organizer of the well known Qatar Epilepsy Symposium, he is running yearly for the past 14 years and which is considered a landmark in the Gulf region; He has also started last year , together with other epileptologists from Qatar, the region and elsewhere, a yearly International Epilepsy School Course, which was attended by many neurologists from the Area.\n\nInternationally, Dr Mesraoua is an active and elected member of the Commission on Eastern Mediterranean Region (EMR ) , a regional branch of the International League Against Epilepsy (ILAE), where he represents the Middle East and North Africa(MENA ) and where he holds the position of chief of the Epilepsy Epidemiology Section; Dr Mesraoua is a member of the American Academy of Neurology, the Europeen Academy of Neurology and the American Epilepsy Society.\n\nDr Mesraoua's main objectives are to encourage frequent gathering of the epileptologists/neurologists from the MENA region and the rest of the world, promote Epilepsy Teaching in the MENA Region, and encourage multicenter studies involving neurologists and epileptologists in the MENA region, particularly epilepsy epidemiological studies. \n\nDr. Mesraoua is the recipient of two research Grants, as the Lead Principal Investigator (750.000 USD and 250.000 USD) from the Qatar National Research Fund (QNRF) and the Hamad Hospital Internal Research Grant (IRGC), on the following topics : “Continuous EEG Monitoring in the ICU “ and on “Alpha-lactoalbumin , proof of concept in the treatment of epilepsy” .Dr Mesraoua is a reviewer for the journal \"seizures\" (Europeen Epilepsy Journal ) as well as dove journals ; Dr Mesraoua is the author and co-author of many peer reviewed publications and four book chapters in the field of Epilepsy and Clinical Neurology",institutionString:"Weill Cornell Medical College in Qatar",institution:{name:"Weill Cornell Medical College in Qatar",country:{name:"Qatar"}}},{id:"282429",title:"Prof.",name:"Covanis",middleName:null,surname:"Athanasios",slug:"covanis-athanasios",fullName:"Covanis Athanasios",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/282429/images/system/282429.jpg",biography:null,institutionString:"Neurology-Neurophysiology Department of the Children Hospital Agia Sophia",institution:null},{id:"190980",title:"Prof.",name:"Marwa",middleName:null,surname:"Mahmoud Saleh",slug:"marwa-mahmoud-saleh",fullName:"Marwa Mahmoud Saleh",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/190980/images/system/190980.jpg",biography:"Professor Marwa Mahmoud Saleh is a doctor of medicine and currently works in the unit of Phoniatrics, Department of Otolaryngology, Ain Shams University in Cairo, Egypt. She got her doctoral degree in 1991 and her doctoral thesis was accomplished in the University of Iowa, United States. Her publications covered a multitude of topics as videokymography, cochlear implants, stuttering, and dysphagia. She has lectured Egyptian phonology for many years. Her recent research interest is joint attention in autism.",institutionString:"Ain Shams University",institution:{name:"Ain Shams University",country:{name:"Egypt"}}},{id:"259190",title:"Dr.",name:"Syed Ali Raza",middleName:null,surname:"Naqvi",slug:"syed-ali-raza-naqvi",fullName:"Syed Ali Raza Naqvi",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/259190/images/system/259190.png",biography:"Dr. Naqvi is a radioanalytical chemist and is working as an associate professor of analytical chemistry in the Department of Chemistry, Government College University, Faisalabad, Pakistan. Advance separation techniques, nuclear analytical techniques and radiopharmaceutical analysis are the main courses that he is teaching to graduate and post-graduate students. In the research area, he is focusing on the development of organic- and biomolecule-based radiopharmaceuticals for diagnosis and therapy of infectious and cancerous diseases. Under the supervision of Dr. Naqvi, three students have completed their Ph.D. degrees and 41 students have completed their MS degrees. He has completed three research projects and is currently working on 2 projects entitled “Radiolabeling of fluoroquinolone derivatives for the diagnosis of deep-seated bacterial infections” and “Radiolabeled minigastrin peptides for diagnosis and therapy of NETs”. He has published about 100 research articles in international reputed journals and 7 book chapters. Pakistan Institute of Nuclear Science & Technology (PINSTECH) Islamabad, Punjab Institute of Nuclear Medicine (PINM), Faisalabad and Institute of Nuclear Medicine and Radiology (INOR) Abbottabad are the main collaborating institutes.",institutionString:"Government College University",institution:{name:"Government College University, Faisalabad",country:{name:"Pakistan"}}},{id:"58390",title:"Dr.",name:"Gyula",middleName:null,surname:"Mozsik",slug:"gyula-mozsik",fullName:"Gyula Mozsik",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/58390/images/system/58390.png",biography:"Gyula Mózsik MD, Ph.D., ScD (med), is an emeritus professor of Medicine at the First Department of Medicine, Univesity of Pécs, Hungary. He was head of this department from 1993 to 2003. His specializations are medicine, gastroenterology, clinical pharmacology, clinical nutrition, and dietetics. His research fields are biochemical pharmacological examinations in the human gastrointestinal (GI) mucosa, mechanisms of retinoids, drugs, capsaicin-sensitive afferent nerves, and innovative pharmacological, pharmaceutical, and nutritional (dietary) research in humans. He has published about 360 peer-reviewed papers, 197 book chapters, 692 abstracts, 19 monographs, and has edited 37 books. He has given about 1120 regular and review lectures. He has organized thirty-eight national and international congresses and symposia. He is the founder of the International Conference on Ulcer Research (ICUR); International Union of Pharmacology, Gastrointestinal Section (IUPHAR-GI); Brain-Gut Society symposiums, and gastrointestinal cytoprotective symposiums. He received the Andre Robert Award from IUPHAR-GI in 2014. Fifteen of his students have been appointed as full professors in Egypt, Cuba, and Hungary.",institutionString:"University of Pécs",institution:{name:"University of Pecs",country:{name:"Hungary"}}},{id:"277367",title:"M.Sc.",name:"Daniel",middleName:"Martin",surname:"Márquez López",slug:"daniel-marquez-lopez",fullName:"Daniel Márquez López",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/277367/images/7909_n.jpg",biography:"Msc Daniel Martin Márquez López has a bachelor degree in Industrial Chemical Engineering, a Master of science degree in the same área and he is a PhD candidate for the Instituto Politécnico Nacional. His Works are realted to the Green chemistry field, biolubricants, biodiesel, transesterification reactions for biodiesel production and the manipulation of oils for therapeutic purposes.",institutionString:null,institution:{name:"Instituto Politécnico Nacional",country:{name:"Mexico"}}},{id:"196544",title:"Prof.",name:"Angel",middleName:null,surname:"Catala",slug:"angel-catala",fullName:"Angel Catala",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/196544/images/system/196544.jpg",biography:"Angel Catalá studied chemistry at Universidad Nacional de La Plata, Argentina, where he received a Ph.D. in Chemistry (Biological Branch) in 1965. From 1964 to 1974, he worked as an Assistant in Biochemistry at the School of Medicine at the same university. From 1974 to 1976, he was a fellow of the National Institutes of Health (NIH) at the University of Connecticut, Health Center, USA. From 1985 to 2004, he served as a Full Professor of Biochemistry at the Universidad Nacional de La Plata. He is a member of the National Research Council (CONICET), Argentina, and the Argentine Society for Biochemistry and Molecular Biology (SAIB). His laboratory has been interested for many years in the lipid peroxidation of biological membranes from various tissues and different species. Dr. Catalá has directed twelve doctoral theses, published more than 100 papers in peer-reviewed journals, several chapters in books, and edited twelve books. He received awards at the 40th International Conference Biochemistry of Lipids 1999 in Dijon, France. He is the winner of the Bimbo Pan-American Nutrition, Food Science and Technology Award 2006 and 2012, South America, Human Nutrition, Professional Category. In 2006, he won the Bernardo Houssay award in pharmacology, in recognition of his meritorious works of research. Dr. Catalá belongs to the editorial board of several journals including Journal of Lipids; International Review of Biophysical Chemistry; Frontiers in Membrane Physiology and Biophysics; World Journal of Experimental Medicine and Biochemistry Research International; World Journal of Biological Chemistry, Diabetes, and the Pancreas; International Journal of Chronic Diseases & Therapy; and International Journal of Nutrition. He is the co-editor of The Open Biology Journal and associate editor for Oxidative Medicine and Cellular Longevity.",institutionString:"Universidad Nacional de La Plata",institution:{name:"National University of La Plata",country:{name:"Argentina"}}},{id:"186585",title:"Dr.",name:"Francisco Javier",middleName:null,surname:"Martin-Romero",slug:"francisco-javier-martin-romero",fullName:"Francisco Javier Martin-Romero",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bSB3HQAW/Profile_Picture_1631258137641",biography:"Francisco Javier Martín-Romero (Javier) is a Professor of Biochemistry and Molecular Biology at the University of Extremadura, Spain. He is also a group leader at the Biomarkers Institute of Molecular Pathology. Javier received his Ph.D. in 1998 in Biochemistry and Biophysics. At the National Cancer Institute (National Institute of Health, Bethesda, MD) he worked as a research associate on the molecular biology of selenium and its role in health and disease. After postdoctoral collaborations with Carlos Gutierrez-Merino (University of Extremadura, Spain) and Dario Alessi (University of Dundee, UK), he established his own laboratory in 2008. The interest of Javier's lab is the study of cell signaling with a special focus on Ca2+ signaling, and how Ca2+ transport modulates the cytoskeleton, migration, differentiation, cell death, etc. He is especially interested in the study of Ca2+ channels, and the role of STIM1 in the initiation of pathological events.",institutionString:null,institution:{name:"University of Extremadura",country:{name:"Spain"}}},{id:"217323",title:"Prof.",name:"Guang-Jer",middleName:null,surname:"Wu",slug:"guang-jer-wu",fullName:"Guang-Jer Wu",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/217323/images/8027_n.jpg",biography:null,institutionString:null,institution:null},{id:"148546",title:"Dr.",name:"Norma Francenia",middleName:null,surname:"Santos-Sánchez",slug:"norma-francenia-santos-sanchez",fullName:"Norma Francenia Santos-Sánchez",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/148546/images/4640_n.jpg",biography:null,institutionString:null,institution:null},{id:"272889",title:"Dr.",name:"Narendra",middleName:null,surname:"Maddu",slug:"narendra-maddu",fullName:"Narendra Maddu",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/272889/images/10758_n.jpg",biography:null,institutionString:null,institution:null},{id:"242491",title:"Prof.",name:"Angelica",middleName:null,surname:"Rueda",slug:"angelica-rueda",fullName:"Angelica Rueda",position:"Investigador Cinvestav 3B",profilePictureURL:"https://mts.intechopen.com/storage/users/242491/images/6765_n.jpg",biography:null,institutionString:null,institution:null},{id:"88631",title:"Dr.",name:"Ivan",middleName:null,surname:"Petyaev",slug:"ivan-petyaev",fullName:"Ivan Petyaev",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Lycotec (United Kingdom)",country:{name:"United Kingdom"}}},{id:"423869",title:"Ms.",name:"Smita",middleName:null,surname:"Rai",slug:"smita-rai",fullName:"Smita Rai",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Integral University",country:{name:"India"}}},{id:"424024",title:"Prof.",name:"Swati",middleName:null,surname:"Sharma",slug:"swati-sharma",fullName:"Swati Sharma",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Integral University",country:{name:"India"}}},{id:"439112",title:"MSc.",name:"Touseef",middleName:null,surname:"Fatima",slug:"touseef-fatima",fullName:"Touseef Fatima",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Integral University",country:{name:"India"}}},{id:"424836",title:"Dr.",name:"Orsolya",middleName:null,surname:"Borsai",slug:"orsolya-borsai",fullName:"Orsolya Borsai",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca",country:{name:"Romania"}}},{id:"422262",title:"Ph.D.",name:"Paola Andrea",middleName:null,surname:"Palmeros-Suárez",slug:"paola-andrea-palmeros-suarez",fullName:"Paola Andrea Palmeros-Suárez",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"University of Guadalajara",country:{name:"Mexico"}}}]}},subseries:{item:{id:"12",type:"subseries",title:"Human Physiology",keywords:"Anatomy, Cells, Organs, Systems, Homeostasis, Functions",scope:"Human physiology is the scientific exploration of the various functions (physical, biochemical, and mechanical properties) of humans, their organs, and their constituent cells. The endocrine and nervous systems play important roles in maintaining homeostasis in the human body. Integration, which is the biological basis of physiology, is achieved through communication between the many overlapping functions of the human body's systems, which takes place through electrical and chemical means. Much of the basis of our knowledge of human physiology has been provided by animal experiments. Because of the close relationship between structure and function, studies in human physiology and anatomy seek to understand the mechanisms that help the human body function. The series on human physiology deals with the various mechanisms of interaction between the various organs, nerves, and cells in the human body.",coverUrl:"https://cdn.intechopen.com/series_topics/covers/12.jpg",hasOnlineFirst:!0,hasPublishedBooks:!0,annualVolume:11408,editor:{id:"195829",title:"Prof.",name:"Kunihiro",middleName:null,surname:"Sakuma",slug:"kunihiro-sakuma",fullName:"Kunihiro Sakuma",profilePictureURL:"https://mts.intechopen.com/storage/users/195829/images/system/195829.jpg",biography:"Professor Kunihiro Sakuma, Ph.D., currently works in the Institute for Liberal Arts at the Tokyo Institute of Technology. He is a physiologist working in the field of skeletal muscle. He was awarded his sports science diploma in 1995 by the University of Tsukuba and began his scientific work at the Department of Physiology, Aichi Human Service Center, focusing on the molecular mechanism of congenital muscular dystrophy and normal muscle regeneration. His interest later turned to the molecular mechanism and attenuating strategy of sarcopenia (age-related muscle atrophy). His opinion is to attenuate sarcopenia by improving autophagic defects using nutrient- and pharmaceutical-based treatments.",institutionString:null,institution:{name:"Tokyo Institute of Technology",institutionURL:null,country:{name:"Japan"}}},editorTwo:null,editorThree:{id:"331519",title:"Dr.",name:"Kotomi",middleName:null,surname:"Sakai",slug:"kotomi-sakai",fullName:"Kotomi Sakai",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y000031QtFXQA0/Profile_Picture_1637053227318",biography:"Senior researcher Kotomi Sakai, Ph.D., MPH, works at the Research Organization of Science and Technology in Ritsumeikan University. She is a researcher in the geriatric rehabilitation and public health field. She received Ph.D. from Nihon University and MPH from St.Luke’s International University. Her main research interest is sarcopenia in older adults, especially its association with nutritional status. Additionally, to understand how to maintain and improve physical function in older adults, to conduct studies about the mechanism of sarcopenia and determine when possible interventions are needed.",institutionString:null,institution:{name:"Ritsumeikan University",institutionURL:null,country:{name:"Japan"}}},series:{id:"10",title:"Physiology",doi:"10.5772/intechopen.72796",issn:"2631-8261"},editorialBoard:[{id:"213786",title:"Dr.",name:"Henrique P.",middleName:null,surname:"Neiva",slug:"henrique-p.-neiva",fullName:"Henrique P. 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