Dimensions of sample holder shapes.
\r\n\tThe book aims to introduce the potential reader to the problems associated with aeronautics, ranging from academic research to actual application and precise work, and to be of interest to those who want to research and build their techniques in the related fields.
",isbn:"978-1-80355-301-6",printIsbn:"978-1-80355-300-9",pdfIsbn:"978-1-80355-302-3",doi:null,price:0,priceEur:0,priceUsd:0,slug:null,numberOfPages:0,isOpenForSubmission:!0,isSalesforceBook:!1,hash:"a6b8e86015392b400a37551116fc0c13",bookSignature:"Associate Prof. Zain Anwar Anwar Ali",publishedDate:null,coverURL:"https://cdn.intechopen.com/books/images_new/11522.jpg",keywords:"Aeronautics, Aircraft, Control System, Surveillance, Guidance, Fixed-Wing, Rotorcraft, Jet Engine, Modern Drone, Path Planning, Adaptive Control, Hybrid Control",numberOfDownloads:null,numberOfWosCitations:0,numberOfCrossrefCitations:null,numberOfDimensionsCitations:null,numberOfTotalCitations:null,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"February 9th 2022",dateEndSecondStepPublish:"April 12th 2022",dateEndThirdStepPublish:"June 11th 2022",dateEndFourthStepPublish:"August 30th 2022",dateEndFifthStepPublish:"October 29th 2022",remainingDaysToSecondStep:"a month",secondStepPassed:!0,currentStepOfPublishingProcess:3,editedByType:null,kuFlag:!1,biosketch:"Engr. Dr. Zain Anwar Ali is working as an Associate Prof. and Editor of Sir Syed University Research Journal of Engineering and Technology. He received research funding from Higher Education Commission (HEC), Pakistan, and has research collaborations with several universities in China, including Nanjing University of Aeronautics and Astronautics, Donghua University, Shanghai University, and South East University, under different research grants provided by the National Nature Science Foundation of China.",coeditorOneBiosketch:null,coeditorTwoBiosketch:null,coeditorThreeBiosketch:null,coeditorFourBiosketch:null,coeditorFiveBiosketch:null,editors:[{id:"415526",title:"Associate Prof.",name:"Zain",middleName:"Anwar",surname:"Anwar Ali",slug:"zain-anwar-ali",fullName:"Zain Anwar Ali",profilePictureURL:"https://mts.intechopen.com/storage/users/415526/images/system/415526.png",biography:"Engr. Dr. Zain Anwar Ali received his B.S. degree in Electronic Engineering from Sir Syed University of Engineering and Technology, Karachi, Pakistan, in 2009. In the same year, he joined Sir Syed UET as a Research Assistant in the Electronic Engineering department, and was soon promoted to a Junior Lecturer due to his hard work and research contributions. He completed his Master's in Industrial Control and Automation at the Hamdard University of Engineering in 2012, securing his second position and soon being promoted to a Lecturer. Later he joined Nanjing University of Aeronautics and Astronautics (NUAA) as a Ph.D. research scholar and the Nanjing Strong Flight Electronics and Machinery LTD to complete his Ph.D. experimental work there. In 2017, he completed his Ph.D. in the field of Control Theory and Control Engineering NUAA. He then rejoined Sir Syed UET as an Assistant Professor in the Electronics Engineering department. In the same year, he was selected as a highly talented foreign expert by the Ministry of China, Beijing, at Liaocheng. After seeing his research background, the vice-chancellor of SSUET gave him the extra responsibility of an Associate Editor of Sir Syed UET research journal which is indexed at various indexing agencies and published in two issues annually. In 2018-2019, he received research funding from Higher Education Commission (HEC), Pakistan, and started some different research collaborations with several universities in China, including Nanjing University of Aeronautics and Astronautics (NUAA-Nanjing), Donghua University (DU-Shanghai), Shanghai University (SU-Shanghai), and South East University (SEU-Nanjing), under different research grants provided by the National Nature Science Foundation of China (NSFC). Currently, Dr. Ali is working as an Associate Professor at the Electronic Engineering Department, Sir Syed University of Engineering and Technology, Karachi, Pakistan, and as the Editor of Sir Syed University Research Journal of Engineering and Technology.",institutionString:"Sir Syed University of Engineering and Technology",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"1",totalChapterViews:"0",totalEditedBooks:"0",institution:{name:"Sir Syed University of Engineering and Technology",institutionURL:null,country:{name:"Pakistan"}}}],coeditorOne:null,coeditorTwo:null,coeditorThree:null,coeditorFour:null,coeditorFive:null,topics:[{id:"11",title:"Engineering",slug:"engineering"}],chapters:null,productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"},personalPublishingAssistant:{id:"347258",firstName:"Marica",lastName:"Novakovic",middleName:null,title:"Ms.",imageUrl:"//cdnintech.com/web/frontend/www/assets/author.svg",email:"marica@intechopen.com",biography:null}},relatedBooks:[{type:"book",id:"10198",title:"Response Surface Methodology in Engineering Science",subtitle:null,isOpenForSubmission:!1,hash:"1942bec30d40572f519327ca7a6d7aae",slug:"response-surface-methodology-in-engineering-science",bookSignature:"Palanikumar Kayaroganam",coverURL:"https://cdn.intechopen.com/books/images_new/10198.jpg",editedByType:"Edited by",editors:[{id:"321730",title:"Prof.",name:"Palanikumar",surname:"Kayaroganam",slug:"palanikumar-kayaroganam",fullName:"Palanikumar Kayaroganam"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"1591",title:"Infrared Spectroscopy",subtitle:"Materials Science, Engineering and Technology",isOpenForSubmission:!1,hash:"99b4b7b71a8caeb693ed762b40b017f4",slug:"infrared-spectroscopy-materials-science-engineering-and-technology",bookSignature:"Theophile Theophanides",coverURL:"https://cdn.intechopen.com/books/images_new/1591.jpg",editedByType:"Edited by",editors:[{id:"37194",title:"Dr.",name:"Theophile",surname:"Theophanides",slug:"theophile-theophanides",fullName:"Theophile Theophanides"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"3161",title:"Frontiers in Guided Wave Optics and Optoelectronics",subtitle:null,isOpenForSubmission:!1,hash:"deb44e9c99f82bbce1083abea743146c",slug:"frontiers-in-guided-wave-optics-and-optoelectronics",bookSignature:"Bishnu Pal",coverURL:"https://cdn.intechopen.com/books/images_new/3161.jpg",editedByType:"Edited by",editors:[{id:"4782",title:"Prof.",name:"Bishnu",surname:"Pal",slug:"bishnu-pal",fullName:"Bishnu Pal"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"3092",title:"Anopheles mosquitoes",subtitle:"New insights into malaria vectors",isOpenForSubmission:!1,hash:"c9e622485316d5e296288bf24d2b0d64",slug:"anopheles-mosquitoes-new-insights-into-malaria-vectors",bookSignature:"Sylvie Manguin",coverURL:"https://cdn.intechopen.com/books/images_new/3092.jpg",editedByType:"Edited by",editors:[{id:"50017",title:"Prof.",name:"Sylvie",surname:"Manguin",slug:"sylvie-manguin",fullName:"Sylvie Manguin"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"371",title:"Abiotic Stress in Plants",subtitle:"Mechanisms and Adaptations",isOpenForSubmission:!1,hash:"588466f487e307619849d72389178a74",slug:"abiotic-stress-in-plants-mechanisms-and-adaptations",bookSignature:"Arun Shanker and B. Venkateswarlu",coverURL:"https://cdn.intechopen.com/books/images_new/371.jpg",editedByType:"Edited by",editors:[{id:"58592",title:"Dr.",name:"Arun",surname:"Shanker",slug:"arun-shanker",fullName:"Arun Shanker"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"72",title:"Ionic Liquids",subtitle:"Theory, Properties, New Approaches",isOpenForSubmission:!1,hash:"d94ffa3cfa10505e3b1d676d46fcd3f5",slug:"ionic-liquids-theory-properties-new-approaches",bookSignature:"Alexander Kokorin",coverURL:"https://cdn.intechopen.com/books/images_new/72.jpg",editedByType:"Edited by",editors:[{id:"19816",title:"Prof.",name:"Alexander",surname:"Kokorin",slug:"alexander-kokorin",fullName:"Alexander Kokorin"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"314",title:"Regenerative Medicine and Tissue Engineering",subtitle:"Cells and Biomaterials",isOpenForSubmission:!1,hash:"bb67e80e480c86bb8315458012d65686",slug:"regenerative-medicine-and-tissue-engineering-cells-and-biomaterials",bookSignature:"Daniel Eberli",coverURL:"https://cdn.intechopen.com/books/images_new/314.jpg",editedByType:"Edited by",editors:[{id:"6495",title:"Dr.",name:"Daniel",surname:"Eberli",slug:"daniel-eberli",fullName:"Daniel Eberli"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"57",title:"Physics and Applications of Graphene",subtitle:"Experiments",isOpenForSubmission:!1,hash:"0e6622a71cf4f02f45bfdd5691e1189a",slug:"physics-and-applications-of-graphene-experiments",bookSignature:"Sergey Mikhailov",coverURL:"https://cdn.intechopen.com/books/images_new/57.jpg",editedByType:"Edited by",editors:[{id:"16042",title:"Dr.",name:"Sergey",surname:"Mikhailov",slug:"sergey-mikhailov",fullName:"Sergey Mikhailov"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"1373",title:"Ionic Liquids",subtitle:"Applications and Perspectives",isOpenForSubmission:!1,hash:"5e9ae5ae9167cde4b344e499a792c41c",slug:"ionic-liquids-applications-and-perspectives",bookSignature:"Alexander Kokorin",coverURL:"https://cdn.intechopen.com/books/images_new/1373.jpg",editedByType:"Edited by",editors:[{id:"19816",title:"Prof.",name:"Alexander",surname:"Kokorin",slug:"alexander-kokorin",fullName:"Alexander Kokorin"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"2270",title:"Fourier Transform",subtitle:"Materials Analysis",isOpenForSubmission:!1,hash:"5e094b066da527193e878e160b4772af",slug:"fourier-transform-materials-analysis",bookSignature:"Salih Mohammed Salih",coverURL:"https://cdn.intechopen.com/books/images_new/2270.jpg",editedByType:"Edited by",editors:[{id:"111691",title:"Dr.Ing.",name:"Salih",surname:"Salih",slug:"salih-salih",fullName:"Salih Salih"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}}]},chapter:{item:{type:"chapter",id:"73991",title:"Development of a Novel Electromagnetic Rewarming Technology for the Cryopreservation of Stem Cells with Large Volume",doi:"10.5772/intechopen.94556",slug:"development-of-a-novel-electromagnetic-rewarming-technology-for-the-cryopreservation-of-stem-cells-w",body:'\nCryopreservation is one the most essential techniques that has been widely used for preserving stem cells in scientific research and cellular therapies [1]. The principle of cryopreservation is to use the super low temperature to reduce the biological and chemical reactions in living stem cells. The expansion in clinical tests for biomedical applications revealed the limitations of the current preservation technologies, i.e., only the small volumes of stem cells can be successfully cryopreserved. In the case of large samples such as bulk volume of cells, tissues, or organs, cryopreservation often fails because of the damage caused by ice crystal growth and thermal stress within the bio-samples [2, 3].
\nIn 1960s, Mazur proposed “two-factor hypothesis” of freezing injury based on the study of Chinese hamster tissue [4]. During the cooling process, two distinct types of cryoinjuries determine the life or death of the cells, which are affected by the cooling rate. Neither too high nor too low the cooling rate is favored for the cryopreservation (Figure 1).
\nSchematic drawing of physical events in cells during freezing.
When cells are cooled down to subzero temperatures under a normal pressure, ice crystals emerge and grow in the suspensions outside the cell membrane in the beginning [5]. The external ice growth into cells is blocked by the plasma membrane. The cytoplasmic region remains unfrozen and in the supercooled state [6]. However, the increase of osmolality and chemical potential difference across cell membranes due to the external ice formation will serve as a driving force for mass transfer between the intracellular components and extracellular environment, pulling water out of cells. If the cooling is too rapid, there is insufficient time for the water to flow out of the cells. As the temperature goes down rapidly, the unfrozen and supercooled state is disturbed and the intracellular ice formation (IIF) happens [7]. The lethal IIF can rupture the cell membranes and lead to the cell death. On the other hand, if the cooling rate is too slow, intracellular ice may be reduced or avoided. The plenty of time permits water transport out of the membrane under the influence of the osmolality difference. Cells then suffer from high concentration of intracellular solute/electrolytes (so called “solution effects”) and severe dehydration. The slower cooling process may expose the cells to “solution effects” for a longer time, which is unacceptable for the cells. Therefore, the cooling rate may not be too high or too low based on the “two-factor hypothesis”.
\nLater in 1984, Mazur reported the rapid rewarming can ‘rescue’ the rapidly frozen cells [6]. Though the fast cooling process produces intracellular ice, the crystals tend to be small. Due to the unstable thermal properties, during rewarming, small crystals formed at lower temperature aggregate to become larger crystals. The process referred to as recrystallization. It has been proved that the cells cooled at rate far beyond the optimal one will survive if warmed at very rapid rate, but cells do not survive if warmed slowly [8, 9]. Besides the rapid rewarming rate, uniformity of temperature distribution also plays an important role to the survival of the samples [10]. Thermal stress caused by the temperature gradients will lead to the crack of the brittle material, especially the larger systems [11]. Thus, both of fast and homogeneity are essential in the thawing. A rapid-uniform rewarming technology is needed to successful cryopreservation of stem cells with large volume.
\nA typical cryopreservation procedure is composed of five steps:
addition of appropriate cryoprotective agents (CPAs),
cooling at an optimal rate,
storage at the low temperature (e.g. -80°C freezer or − 196°C liquid nitrogen tank),
rearming to physiological temperature, and
removal of the CPAs.
Over the past decades, scientists made significant progress in the methods and protocols of addition/removal of CPAs, approaches of cooling to the target temperature, and strategies to preserve biomaterials at sub-zero temperature environment. However, in the scientific and clinical applications, convective rewarming of the cryopreserved samples, typically thawing in a 37°C water bath, remains the gold standard for small samples [12].
\nWarming in the water bath is a convective rewarming approach in which heat is transferred from the outer boundaries to the inner portion of the cryopreserved biological samples. Because of the high specific heat of biological materials, it requires a great amount of heat to rewarm them. Another obstacle for the rewarming process is the low thermal conductivity of biomaterials. Heat cannot be quickly transmitted into the core area of large samples. For a small volume of cell suspensions, the problem caused by the lower thermal conductivity may possibly be solved by the design of sample holder (e.g. maximize the sample holder’s ratio of surface area to volume) [13]. However, for larger system with much smaller ratio of the surface area to the volume, they cannot be sliced or pressed into a thin film to increase the heat transfer area for convective warming in water bath. In this case, a large temperature difference will occur and lead to thermal stresses that can result in fracture of the samples during rewarming. Therefore, the traditional method of convective rewarming at the sample surface is not appropriate for the cryopreservation of large volume of biomaterials. A uniform and rapid rewarming method is needed to meet the urgent needs in tissue engineering and cellular therapy, which may be achieved by a volumetric rewarming technique, e.g., electromagnetic heating.
\nThe electromagnetic field is a combination of the electric field and the magnetic field generated by electrically charged objects. The electric field and magnetic field are coupled with each other. A time varying electric field (contrary to static field) induces magnetic field changing over time, and vice versa. The distribution and propagation of electromagnetic fields are governed by Maxwell’s equations. An electromagnetic wave is the propagation of the electromagnetic field through media such as air, water, etc., or in vacuum. This propagation also refers to radiation transmitting the electromagnetic energy, momentum and angular momentum through space. As one of the basic forces in nature, the propagating electromagnetic fields interact with other materials and generate different effects depending on the frequency and power. Based on the mechanisms behind these interactions between electromagnetic waves and other materials, electromagnetic waves have been applied in numerous aspects including: telecommunications such as mobile phone calling, the short wave broadcast, TV signal transmission and connection between the spaceship and the base on earth, remote sensing for the weather forecasting, land mapping, infrared radiation detecting, X-rays and computer tomography (CT) or the disease diagnoses, and radiotherapy which employs higher energy radiation to kill cancer cells. These various applications are closely related to the spectrum of electromagnetic waves. The most commonly used frequency band is at radio frequency (RF) electromagnetic wave and microwave.
\nThe thermal effects of electromagnetic waves benefit the organism on earth even long before the prehistory. The thermal energy transmitted by the electromagnetic waves emitted from the sun allows the survival of plants, animals. This electromagnetic wave emitted from the sun, or sunlight, is distributed across almost the entire spectrum but with major intensity on infrared, visible, and ultraviolet frequency ranges. These major components visible light has a frequency range in several hundreds of THz. Higher frequency electromagnetic waves such as X-rays carry much higher energy and are used for radiation therapy killing the tumor cells by ionizing the molecules. In the lower frequency range, electromagnetic waves (radio frequency electromagnetic waves, microwave) with less energy can hardly excite ionization, but can result in thermal effects with materials.
\nThe investigation of the heating phenomenon by microwave occurred in the 1940s. A candy bar under an active microwave generating devices melted and the engineer used this observation to develop equipment for preparing and heating food utilizing the thermal effects of microwave radiation. Since then, not only has the commercialization of the microwave oven become widespread, scientists also began to study the potential benefits for industry and medicine. Most of food is a dielectric material that can interact with the electromagnetic fields in the microwave oven due to a large portion of water in the contents. The water molecules are dipole molecules comprising an electrical positive charged end and an electrical negative charged end. Under the influence of electric fields, these dipole molecules will align themselves with the applied electric fields. When the directions of external electric fields are changing as in the oscillating electromagnetic waves, the dipole molecules will rotate to follow up with the changing fields. The interactions between the rapid changing electromagnetic fields and water dipole molecules will cause mechanical friction forces among the water molecules. The frictions between these micro molecules generate heat volumetrically. Similarly, to the food in the microwave oven for heating, the biological materials in cryopreservation are organisms that are primarily composed of water. Therefore, microwave can also be used for the heating of cryopreserved biological systems.
\nWhen using microwave as the rewarming approach in the cryopreservation, the biomaterials interact with the applied electromagnetic field. Since most biomaterials are nonmagnetic, the forced movement of molecules is mainly due to the electric field component of the electromagnetic field. As shown in Figure 2, a distinct advantage of microwave rewarming compared with the traditional water bath rewarming is that the heat is generated over the entire region of the material which can lead to volumetric and uniform heating.
\nIllustration of heat generation under the influence of electromagnetic waves.
The electrical parameter that characterizes the interaction between the material and electric field component is the relative complex permittivity, or dielectric property, expressed in Eq. (1):
\nThe real part of complex permittivity, \n
where \n
The development of electromagnetic rewarming systems is limited by the cost and inadequate theoretical guidance. The establishment of an electromagnetic resonance rewarming system involves the selection of frequency source and power, manufacturing of the resonance chamber, optimization of the electromagnetic energy feeding approach to the cryopreserved materials. Setting up a specific resonance rewarming system can take a few months to years and requires substantial funding support. When the system is going to be scaled up to materials of a larger dimension, various parameters of the system should be replaced which will extend the time required for the optimization. The first experimental investigation of using electromagnetic energy in cryopreservation began in the 1970s. In Kettrer’s experiment [14], 20 kidneys rewarmed by a microwave generation device with power control were considered partial success. By using a commercial 1.35 kW Toshiba microwave oven which generated by 2.45 GHz magnetron, Guttman [15] reported the electromagnetic rewarming of 16 cryopreserved canine kidneys. Half of the dogs receiving transplantation of these kidneys survived months.
\nHowever, in Pegg’s attempt [16] to repeat the rewarming of dog kidneys with commercial microwave oven, none of the post-thawing dog kidneys function properly.
\nAnother device designed by Burdette [17] generated an electromagnetic field with an open electromagnetic illumination system. The frequency can be adjusted to several distinct values. The rewarming results of rabbit and canine kidneys were published without the following viability analysis.
\nThese preceding explorations opened a new avenue for cryobiologists, most specialized in biomechanical and biochemical physics, to implement new technologies from electrical engineering in the application of cryopreservation. A major problem for these early investigations of electromagnetic rewarming is that the frequency of the commercial microwave oven is too high to penetrate into the inner part of the cryopreserved biomaterials. A good uniformity should be achieved with lower frequency electromagnetic waves. In addition, electromagnetic rewarming can result in a ‘thermal runaway’ problem because the dielectric loss of biomaterials generally increases with temperature during rewarming process, which leads to an increasing temperature difference across the sample volume.
\nThermal runaway phenomenon is depicted in Figure 3. Due to the complex interactions between the material and the applied electromagnetic wave, and nonuniformity of material properties, the temperature distribution may not be that uniform initially. The slightly warmer area of the biological samples has a higher ability to absorb energy from electromagnetic waves and convert into heat than colder area. The temperature difference between these components will be magnified. When the difference in governing properties is significant and favors the ultrafast thawing of the hot area, the nonuniform temperature gradient is even intensified.
\nIntroduction of the ‘thermal runaway’ phenomena: The appearing of large temperature gradient by electromagnetic rewarming.
To avoid the localized warming associated with ‘thermal runaway’ and limited penetration depth for the 2.45 GHz high frequency electromagnetic waves, more delicate controlled electromagnetic rewarming systems operating in lower frequencies are required for cryopreservation. Thereafter, a rewarming system using helical coil to generate tens of MHz electromagnetic waves was used to rewarm CPA solutions as a preliminary trial [18]. But the rewarming rate was moderate achieved by this open system. Later on, a few scientists reconsidered the electromagnetic rewarming in closed systems to confine electromagnetic energies [19, 20]. Unlike the commercial microwave oven in which the generated signal shifted around ±50 MHz, the electromagnetic wave was synthesized by voltage-controlled oscillators. The stability of frequency was improved so that the design of chamber could fit with the electromagnetic source and establish resonant state to concentrate the electromagnetic energy.
\nA major problem for these resonant systems may come from an intention to reduce the non-uniformity by adopting multimode resonant cavities. In Rachman’s electromagnetic resonance cavity rewarming system [19], two resonant states of the cavity were excited (TE111 and TM010). Another multimode cavity rewarming system designed by Robinson [20] excited three different modes. The results of the warming test for CPA solutions were improved while the spatial temperature difference was not greatly reduced, which means thermal runaway could not be eliminated. The reason behind this is due to the interaction between the cryopreserved CPAs and the properties of the resonant cavity. Multimode cavities resonating at different frequencies bring more difficulties in the control of signal frequency at each port to feed electromagnetic energy into the cavity. Hence, a single mode resonant cavity which excites only at a specific frequency may be superior in the control of field distribution in the rewarming process.
\nOur goal is to achieve effective cryopreservation protocol for bulk volume of stem cells. In this chapter, the target is to improve electromagnetic rewarming systems for the rapid-uniform rewarming, laying down the foundation for the cryopreservation of biomaterials with large volume.
\nThe dielectric properties of the biomaterials characterize the interaction between applied electromagnetic field and the biomaterials, and thereby determine the absorption of electromagnetic energy by the biomaterials [21, 22]. The dielectric properties are temperature dependent. If the warmer part of the biomaterial absorbs more heat, the temperature at that warmer part would be further increased, increasing temperature gradients, and therefore inducing thermal stresses. A large thermal stress can destroy the viability of cryopreserved materials and can be even more threatening to bulky systems [23, 24]. Since the dielectric properties play a key role in the absorption of electromagnetic energy, it is a priority to discover the dielectric properties of the biomaterials. In cryopreservation, particularly in vitrification using a high concentration cryoprotective agent, the CPA/vitrification solutions dominate the properties of the cell suspensions or tissues. Hence, the dielectric properties of the CPA/vitrification solutions should be determined so that electromagnetic rewarming can be optimized.
\nThe measurement of the dielectric properties of biomaterials requires sensing and monitoring tools. In many biomedical applications, various measurement methods including transmission and reflection techniques have been used to determine dielectric properties [25, 26, 27, 28, 29].
\nThe samples measured by transmission and reflection methods usually are fixed without morphologically change. But in the application of electromagnetic rewarming, the measurement of dielectric properties must be carried out in the subzero temperature range which may involves phase change and rules out the possibility using transmission and reflection techniques. The cavity perturbation method has been used for measuring the electric properties of different kinds of materials [30, 31, 32, 33] due to its ability to measure the dielectric properties of low loss dielectric materials [34]. In the subzero temperature range, the dielectric properties of biomaterials and CPA/vitrification solutions can be very small. Therefore, in this work, we adopted a cavity perturbation method to determine the dielectric properties of three different vitrification solutions at low temperatures. Briefly, a resonant cavity was designed and manufactured to measure the dielectric properties of cryopreserved biomaterials at 434 MHz. By inserting samples with different permittivity into the resonant cavity, the resonant frequency and quality factor could be changed. From the variation of the resonant frequency and the quality factor, the dielectric properties can be derived.
\nThrough the change of resonant frequency ∆\n
where ∆\n
\n\n
The experimental system is shown in Figure 4. A rectangular single-mode resonant cavity resonating at around 434 MHz was manufactured. The dimension of the cavity was designed to 680 × 400 × 350 mm. Copper plates were used to manufacture the cavity due to its high conductivity (to prevent electromagnetic leakage).
\nSchematic of the system for measurement of dielectric properties.
Water, methanol, ethanol, 1-propanol, 2-propanol, ethylene glycol and cyclohexane samples with known properties were used for calibration of the cavity to determine \n
Once \n
To determine the temperature-dependent specific heat capacity, Differential Scanning Calorimetry (DSC) was used to take accurate measurement of various sample solutions. Isothermal step scan method is adopted to minimize the experimental error. The latent heat was incorporated into the effective specific heat capacity when the phase transition occurs.
\nThermal conductivity of sample solutions was measured using a micro thermal sensor developed by Liang, et al. [38] and manufactured in the lab. The sensor works on the principle of Transient Hot Wire (THW). This miniaturized device utilizes a SiO2/Au/SiO2 sandwiched structure to protect the microfabricated serpentine gold coil, which functions as both the heater and a passive thermometer. The sensor has already been tested and shown to measure thermal conductivity of biomaterials and solutions with high accuracy, repeatability and system reliability.
\nPrevious cryobiologists have developed electromagnetic cavity rewarming for large biomaterials. Evans [39], Robinson [37] and Luo [40] built electromagnetic heating systems that could resonate at around 434 MHz but working at different modes. The sample was placed at the center with a large magnitude of the electric field, which is critical to rapid rewarming. The size of the sample was controlled to reduce temperature gradients resulted from the electromagnetic field attenuated away from the center. For multimode resonant cavity rewarming system, it could be difficult to control the resonant state or field distribution with several EM power inputs. While single mode cavity system is easier to concentrate a strong EM field and control in the rewarming process. Therefore, multimode resonant rewarming systems are excluded for optimization here. According to the warming results, electromagnetic rewarming has already demonstrated more effective than traditional water bath method. However, further improvement of the electromagnetic rewarming system is still needed to optimize the rewarming protocol of bulk volume of cells.
\nMultiple theoretical analysis and numerical simulations had been accomplished [41, 42, 43] on different design possibilities of electromagnetic rewarming systems. Method of moments (MoM) was implemented to calculate and analyze the electric filed intensity and profile [42] and the method of finite-difference time-domain (FDTD) was applied to investigate the post-thawed temperature profile [44]. Another attempt involving the resonance rewarming in the numerical model combined with nanoparticles to improve the warming rate. However, in this study, the model was based on the power consumption at 8000 W [45] that is hard to achieve in the real-world.
\nIn this section, a more efficient and effective model based on finite element method combining electromagnetic wave propagation and heat transfer process was presented. The optimization of the shape of cryopreserved sample was performed numerically. The essential physical properties of several sample solutions including complex permittivity, specific heat, and thermal conductivity characterized experimentally in the previous studies were used in this simulation test.
\nThe electromagnetic resonant system setup showed in Figure 5 consists of a signal generator with voltage control at lower scale, a power oscillator, a resonant cavity, a coaxial transmission wire, and a temperature sensor. An antenna made of high electrical conductivity material was used to excite the electric field and create a standing wave pattern of electromagnetic field inside the rewarming cavity. A sample holder contained cryopreserved stem cells or other biomaterials was place in the center of the cavity, where the strongest electric field was established to achieve the fast rewarming.
\nSchematic description of the experimental setup of the EM rewarming system.
Most of the cryopreserved biomaterials are insensitive to the magnetic field, e.g. the stem cells, with electrical parameters including, ε (absolute permittivity, \n
where E is the electric field intensity (\n
where f (Hz) denotes the frequency of the electromagnetic field. q (\n
where \n
In this numerical modeling analysis, the thermal properties of the cryopreserved material were preset to be temperature-dependent. The specific heat and thermal conductivity at different temperatures were captured once the temperature profile in the Eq. (11) was determined. With the updated thermal and electrical properties over the interested temperature range, the electromagnetic field profile was calculated. Cryopreserved material’s dielectric loss \n
The numerical modeling was setup with COMSOL Multiphysics (COMSOL, Burlington, MA, USA), applying the finite element method. Adopting the analytical process discussed in the theoretical formulation section, the thermal science and combined electromagnetic rewarming system in the resonant chamber as shown in Figure 6. In this numerical investigation, the thermal and electrical parameters of the model were determined follow the methods discussed in the previous section.
\nSchematic description of the simulated resonant EM rewarming cavity.
Nyquist criterion was applied to the meshing grid of the simulation. The maximum size of the element grid was contained smaller than half of the wavelength of the electromagnetic wave. Six grids per wavelength in the finite element analysis was also employed to solve the Maxwell’s equation [47].
\nTetrahedral grids were selected in this study due to smaller grid size comparing to the discretization of the resonant rewarming system. Once the tetrahedron grid applied, the grid size of the resonant chamber was calculated to be less than 10% of the wavelength of electromagnetic wave. In the mesh preparation (shown in Figure 7), different meshing approaches were adopted for different components of the system. Refined meshes were created near the boundaries of surfaces (in total, about 988,000 elements were generated). The area around probe antenna and central part of the sample holder were treated with the enhanced meshing. A smaller grid size (about 163,000 elements) will diminish the difference of the temperature profile less than 0.5% over the entire field.
\nGeometric gridded model of the resonant cavity for simulation.
The prepared simulation model was validated with the analytical results. In the model, the resonant frequency of the chamber was 434.767 MHz, consistent with the frequency from the analytical result. When the chamber was at the resonant state, the normalized electric field intensity profile was plotted for both of the simulated result and analytic result in the resonant cavity was plot in comparison with the analytic solution (Figure 8), which proofed a good alignment between two results. As the position advancing to the central part of the cavity, the intensity of the electric field increased significantly. Hence, the cryopreserved bio-sample was designed to be placed in the central to achieve a fast rewarming process by absorb more electromagnetic power.
\nDistribution of the electrical field magnitude at the central cross-sectional plane in the electromagnetic rewarming chamber. Analytical and simulated results show that electric field energy is focused in the center of the cavity.
The effect of sample holder’s shape to the rewarming was studied to minimize the injury to the biomaterials caused by non-uniform thawing. On the other side, different sample holders might be selected based on the size and structure of the biomaterials. Considering the hardness to accurate calculate the energy conversion between the cavity and the sample through analytical solution, and precise temperature profile monitoring over the entire sample space through experiment, in this simulation, a total of four different sample holder shapes (geometry details shown in Table 1) with the same sample volume of 25 mL were calculated to study the difference over the rewarming process. The heating started at the initial temperature set to −80° C and ended at 0°C. The average rewarming rates of cylindrical, ellipsoidal, spherical, and cubic holders were 72.1, 63.5, 46.1, and 22.8° C/min, respectively. From the fast rewarming perspective, cylindrical and ellipsoidal shapes were the top choices.
\nParameter | \nValue (mm) | \n
---|---|
Radius of sphere | \n18 | \n
Radius of cylinder | \n18 | \n
Height of cylinder | \n20 | \n
a-Semi axis of ellipsoid | \n23 | \n
b-Semi axis of ellipsoid | \n14 | \n
c-Semi axis of ellipsoid | \n18 | \n
Side length of cube | \n29 | \n
Dimensions of sample holder shapes.
Later, the temperature profile at the end of the rewarming was investigated. As shown in Figure 9, the temperature gradients that defined as the temperature difference between the maximum and minimum temperatures in the holder divided by the ferret diameter, for spherical, ellipsoidal, cylindrical, and cubic samples were 0.27, 0.62, 0.95 and 1.24° C/mm, respectively. From the temperature gradient perspective, the spherical and ellipsoidal shapes were the top choices.
\nSimulated post thawing temperature distribution of cryopreserved sample solution in different sample holder shapes. (a) Spherical shape; (b) ellipsoidal shape; (c) cubic shape; (d) cylindrical shape. The spherical and ellipsoidal samples manifested more uniform temperature distribution than cubic and cylindrical samples.
Both rewarming rate and temperature distribution results indicated the cubic shape will not perform well the resonant chamber, which aligned with the prediction that samples with sharp edges or surfaces were inappropriate to be heated by the electromagnetic rewarming method [42].
\nRobinson et al. presumed that ellipsoidal sample shape works well for electromagnetic rewarming technology and performed the experiment with a cone-shape sample to approximate a ellipsoidal holder [21]. The currents from numerical modeling confirmed the fast and uniform rewarming of the ellipsoidal sample. However, it is extremally hard to manufacture a precise ellipsoidal shape holder in the real-world. The same hardness also applied to the spherical holder. Moreover, a cryopreserved material should be stored well in an optimized holder to achieve a better rewarming performance. Thus, the material of the holder was ideally a thin-layer to diminish the absorption of the electromagnetic power. Overall, it is an engineering challenge to manufacture a qualified sample holder with desired material and dimensions in spherical or ellipsoidal geometry. Additionally, an extra supporting structure is required to hold the position of the spherical and ellipsoidal sample in the center of the cavity, which may result as the effect of the electromagnetic profile. Therefore, cylindrical holder was the best option with the fair manufacturing and faster rewarming rate. Though, more improvements should be considered to enhance the uniformity of the temperature profile.
\nConvective warming methods are hindered by the poor abilities to conduct heat into the core part of the materials. While volumetric heating method is needed for bulky material, the previous multimode or commercial microwave systems could not be adopted since they lack a precise control system to maintain the resonant state, leading to either recrystallization or devitrification. Due to the slow heating, most of electromagnetic energy was reflected back from the cavity or causing thermal runaway problems and creating undesired hot spots.
\nThere are two major limitations for electromagnetic resonance system to achieve rapid and uniform heating. Firstly, the system itself should provide sufficient electromagnetic energy to warm up biomaterials. Secondly, the temperature dependent dielectric properties of the biomaterials progressively shift the resonant frequency of the resonant chamber during the rewarming process. Therefore, when using resonant electromagnetic field as the heating source, if the electromagnetic signal parameters remain stagnant according to the frequency change resulted from the temperature change of biomaterials, the electromagnetic energy generated may not be converted into the strong electromagnetic field in the resonant chamber to excite resonance.
\nMoreover, if the electromagnetic system source remains static during the rewarming procedure, severe problems regarding the system safety and efficiency may emerge. A higher portion of reflected electromagnetic power can lead to the damage to the system components as well as potential electromagnetic radiation hazards to the surrounding operators. On the other hand, with smaller electromagnetic energy remaining inside the rewarming chamber, sufficiently strong electromagnetic field inside the resonant cavity could hardly be excited resulting a slow warming. Therefore, it requires delicate control on the set up of the electromagnetic resonant system.
\nThe electromagnetic signal is synthesized by a signal generator (Agilent, Santa Clara, CA, USA). The signal generator can generate continuous electromagnetic waves between 250 kHz and 3 GHz which covers lower frequency band of radiofrequency and microwave. And the power output range is +7 to −120 dBm (i.e. 0.005 to 10–15 W). At this low power output, the electromagnetic field established in the resonant chamber is too weak to rewarm the cryopreserved biomaterials rapid enough avoiding devitrification. In order to intensify the electromagnetic field to achieve higher rewarming rates, a power amplifier (OPHIR RF, Los Angeles, CA, USA) was adopted to increase the electromagnetic power to over 57 dBm (501 W).
\nThe power amplifier has a frequency range between 300 and 500 MHz, which fully covers the working frequency range for this experimental investigation. The reflected power received by this power amplifier would also be detected by the control circuits. The power amplifier will automatically cut off excessive output generation to protect itself. Due to the relatively high electromagnetic power used in the system, two side panel cooling fans were incorporated to avoid internal circuits overheating and system shutdown. The connections between the signal generator and power amplifier, as well as other microwave components are through 50 Ω coaxial cables. These cables would have some attenuation effects. Thus, in order to maintain the high power signal from the amplifier to the rest part of the system, the length should be as short as possible. The measurement was done by the signal generator and a power meter. According to the measurements of six coaxial cables of different lengths, the attenuation for coaxial cables is around 0.1 dB/m.
\nThe most significant difference between the current resonance system and the previous assembled circuits is attributed to the frequency tracking component. During the rewarming process, the resonant frequency of the resonant cavity with biomaterials would change on account of the temperature dependent dielectric properties of the inside biological samples. To prevent the mismatch between the synthesized electromagnetic source frequency and the resonant frequency of the rewarming chamber, the generated frequency source should be dynamically adjusted during the rewarming process.
\nIn order to prevent the frequency mismatch between the signal generation and the resonant frequency which is swiftly altered by the massive cryopreserved materials inside, a dynamic feedback control component was added between the electromagnetic source and the resonant cavity. A directional coupler was introduced to sample the transmitted and reflected power. A spectrum analyzer was connected to the port corresponding to the reduced reflected power. The entire spectrum of the reflected power was evaluated and by looking for the highest power peak, the frequency corresponding to the most significant reflected power was determined. During the rewarming process, the frequency generated from the electromagnetic signal source is dynamically changed corresponding to this spectrum and minimize the reflected power. Otherwise, the large portion of reflected power could lead to a slow warming rate with less power into the cavity. In addition, the reflected power can cause a serious damage to the rest part of the electromagnetic resonance system itself, such as the amplifier, signal synthesizer.
\nIn the numerical simulation model, the probe length was adjusted to be the original probe length and the extended probe length. The electric field intensity excited in the sample inside the cavity was calculated. As shown in Figure 10, the electric field intensity in the cryopreserved material increases almost ten times larger than that using the original probe antenna, which suggests that the impedance matching between the loaded cavity and the electromagnetic source is greatly improved by adopting an extended probe antenna.
\nElectric field intensity comparison.
Since electric field power is proportional to the square of the electric field intensity, we could have much more power to heat the material using the optimized extended probe antenna. This numerical estimation of the electric field gives guidance to the experiment, the reflected power was measured by a network analyzer, and the quality factor of the loaded cavity was determined based on the reflection coefficient. It is found the quality factor of the loaded cavity was improved from 1681 to 5577 after adding the probe extension, which can establish a much stronger electromagnetic field inside the cavity for the rapid rewarming of the cryopreserved biomaterials.
\nTo experimentally evaluate the heating performance of the electromagnetic resonance system, a rewarming test of the cryopreserved Jurkat cells with large volume (25 mL) was performed. Jurkat cell is an easily accessible cell line and shares similar cryopreservation protocol to the stem cells. The testing results will guide us the future trails of cryopreservation of large system of stem cells.
\nThe CPA cocktail contains 10% (w/v) dimethyl sulfoxide (\n
Human T lymphocyte leukemia cells (Jurkat cells) were used in the rewarming test. The Jurkat cell lines were purchased from American Type Culture Collection (Manassas, VA). Cells were cultured in the incubator that setting at 37°C, 5% carbon dioxide, and proper humidity. T25 flasks were used during the cell culture. A unit of the growth medium was prepared of 450 mL RPMI medium (life technologies), 50 mL fetal bovine serum (FBS), 5 mL Penicillin–streptomycin, and 5 mL L-glutamine.
\nCylindrical sample holder contains Jurkat cell suspension was placed in a Styrofoam box, then the box was transferred to the −80°C freezer and stored there overnight. The average cooling rate was 2–3°C/min.
\nThe temperature measurements were conducted by a fiber optic temperature meter (Neoptix Inc., Ville de Quebec, QC, Canada) during the rewarming process. The major challenge for the temperature measurement lies in the penetration through cavity wall. The cryopreserved sample remains in the center of the resonant chamber where the highest electromagnetic field was formed. However, the penetration through the cavity wall would undermine the quality factor of the resonant cavity, which means lower portion of electromagnetic energy remained inside the chamber for rewarming. Additional waveguide was designed to allow for the fiber optic temperature sensor to get through the chamber wall and maintain the quality factor at the same time. This waveguide was designed to have the cutoff frequency higher than the operating frequency during the rewarming process. Although the side effects associated with the EM waves are still in debate, it is nevertheless safer to keep away from the possible side effects caused by electromagnetic energy leakage.
\nAt the end of the rewarming process, the surface temperature profile was recorded by an infrared temperature sensor (FLIR systems, Wilsonville, Oregon, USA). The temperature data in the central part of the cryopreserved sample recorded by the fiber optic meter and the surface temperature profile are combined to analyze the temperature gradient.
\n\nFigure 11 shows the comparison of rewarming process of Jurkat cells between conventional water bath and electromagnetic resonance system. The average rewarming rate of water bath was 40°C/min, while increased to 90°C/min for the EM system.
\nRewarming process of Jurkat cell.
Membrane integrity was obtained by Trypan Blue (Sigma-Aldrich) staining to determine the recovery rate of Jurkat cells. Figure 12 shows the comparison of recovery rate of Jurkat cells between conventional water bath and our electromagnetic resonance system. Water bath achieved the Jurkat cell’s recovery rate at \n
Recovery rate of the Jurkat cell.
The post-thawed cell suspensions were cultured in a 37°C incubator with 5% carbon dioxide and proper humidity. As shown in Figure 13, after three days incubation, no significant change was noted to the normalized proliferation rate for the electromagnetic rewarming method. This indicates EM system does not affect Jurkat cell’s cellular functionality.
\nProliferation rate of the post-thawed Jurkat cell.
In this chapter, we provide detailed information about using electromagnetic resonance system to achieve rapid-uniform rewarming in cryopreservation of stem cells. The importance of rapidly and uniformly rewarming process to the bulky system of stem cells was explained, principles of electromagnetic warming were described, essential physical properties of CPA solution and resonance cavity were covered. Theoretical analysis and numerical simulation were introduced to improve the heating performance. A dynamic resonance frequency monitoring and control system was developed. Apart from analytical analysis, a rewarming test of Jurkat cell was performed to experimentally evaluate the electromagnetic rewarming technology. A comprehensive section on cryopreservation of large volume of stem cell has been tried to prepare, and it is aimed to provide insights about rapid-uniform rewarming during cryopreservation.
\nMushrooms are highly perishable in nature and subjected to change in ways that make them unacceptable for human consumption. It has high water content (85–95%), which is lost rapidly by evaporation and transpiration making mushroom discolored, disfigured and unfit for consumption. The rate of water loss depends on mushroom structures, relative humidity, temperature, air movement and atmospheric pressure during storage. Mushrooms represent one of the most perishable commodities, being so delicate by nature and hence need special postharvest treatments. A number of physiological processes take place in freshly cut mushrooms when it is stored (pileus and veil opening, stipe elongation, browning, etc.) resulting in maturation, senesce, and decrease in commercial and nutritional values [1, 2]. Burton et al. [3] found varying changes in color, size, clearness, firmness, maturity stage, blemish-free, flavor, nutritional value and safety by pre-harvest treatments, postharvest processing and storage conditions. The major limitations in mushroom marketing include wilting and shriveling due to rapid water loss, which render them unfit for marketing and consumption [4]. The shelf life of mushroom can often be extended by pretreatments and/or storage at chilling temperatures (above freezing and below 0°C), chemical preservation, and drying processes. Adsule et al. [5] reported the preservation of
Effect of dipping treatment on the quality and shelf life of the button mushroom (
Effect of dipping treatment in different concentrations of EDTA solutions and KMS was studied on weight loss and other quality parameters and shelf life of four varieties/strains of
Loss in weight of fruit bodies of
Treatment | Weight loss % | Texture (days) | Whiteness (days) | Gill opening (days) | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
First year | Second year | Average | First year | Second year | Average | First year | Second year | Average | First year | Second year | Average | |
EDTA 75 ppm | 0.15 | 2.15 | 1.15 | 3 | 4 | 3.5 | 2 | 6 | 4 | 3 | 5 | 4 |
EDTA 125 ppm | 0.27 | 1.78 | 1.02 | 3 | 4 | 3.5 | 2 | 6 | 4 | 4 | 5 | 4.5 |
EDTA200 ppm | 0.42 | 1.85 | 1.13 | 3 | 4 | 3.5 | 3 | 6 | 4.5 | 4 | 5 | 4.5 |
EDTA 500 ppm | 0.33 | 0.97 | 0.56 | 3 | 4 | 3.5 | 2 | 5 | 3.5 | 3 | 5 | 4 |
EDTA 75 ppm | 0.48 | 0.82 | 0.65 | 10 | 14 | 12 | 11 | 16 | 13.5 | 11 | 15 | 13 |
EDTA 125 ppm | 3.41 | 0.68 | 2.04 | 10 | 14 | 12 | 9 | 16 | 12.5 | 10 | 15 | 12.5 |
EDTA200 ppm | 0.53 | 0.55 | 0.54 | 11 | 13 | 12 | 11 | 16 | 13.5 | 11 | 15 | 13 |
EDTA 500 ppm | 0.50 | 0.70 | 0.6 | 8 | 13 | 11 | 9 | 11 | 10 | 9 | 15 | 12 |
Effect of dipping treatment on weight loss, whiteness, texture and gill opening of
The fruiting bodies of
Weight loss % | Texture (days) | Gill opening (days) | Whiteness (days) | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
First year | Second year | Average | First year | Second year | Average | First year | Second year | Average | First year | Second year | Average | |
EDTA 75 ppm | 0.76 | 0.88 | 0.82 | 4 | 4 | 4 | 4 | 5 | 4.5 | 3 | 4 | 3.5 |
EDTA 125 ppm | 0.84 | 1.24 | 1.04 | 4 | 4 | 4 | 4 | 5 | 4.5 | 3 | 4 | 3.5 |
EDTA200 ppm | 0.93 | 1.23 | 1.08 | 4 | 4 | 4 | 4 | 5 | 4.5 | 3 | 4 | 3.5 |
KMS 500 ppm | 0.77 | 1.32 | 1.04 | 4 | 4 | 4 | 4 | 5 | 4.5 | 3 | 4 | 3.5 |
EDTA 75 ppm | 0.93 | 0.60 | 0.76 | 16 | 14 | 15 | 16 | 13 | 14.5 | 16 | 16 | 16 |
EDTA 125 ppm | 0.88 | 0.72 | 0.8 | 16 | 14 | 15 | 16 | 7 | 11.5 | 11 | 16 | 16 |
EDTA200 ppm | 0.53 | 0.58 | 0.55 | 16 | 14 | 15 | 16 | 8 | 12 | 12 | 16 | 14 |
KMS 500 ppm | 0.49 | 0.48 | 0.48 | 16 | 12 | 14 | 16 | 8 | 12 | 16 | 10 | 13 |
Effect of dipping treatment on weight loss, whiteness, texture and gill opening of
Effect of dipping treatment with different concentration of EDTA (75, 125, 200 ppm) and KMS (500 ppm) was studied on weight loss and other quality parameters in strain
Treatment | Weight of fruiting bodies (g) | |||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Initial | 1DA | 2DA | 3DA | 4DA | 5DA | 6DA | 7DA | 8DA | 9DA | 10DA | 11DA | 12DA | 13DA | 14DA | 15DA | 16DA | 17DA | 18DA | Over all reduction | |
EDTA 75 ppm | 40.56 | 40.29 (0.27) | 40.02 (0.27) | 39.72 (0.30) | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | 0.84 |
EDTA 125 ppm | 38.71 | 38.38 (0.33) | 38.01 (0.37) | 37.67 (0.34) | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | 1.04 |
EDTA 200 ppm | 47.06 | 46.78 (0.28) | 46.51 (0.27) | 46.25 (0.24) | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | 0.81 |
KMS 500 ppm | 39.37 | 38.99 (0.38) | 38.65 (0.34) | 38.30 (0.35) | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | 1.07 |
EDTA 75 ppm | 25.09 | 25.03 (0.06) | 24.99 (0.04) | 24.95 (0.04) | 24.90 (0.05) | 24.86 (0.04) | 24.81 (0.05) | 24.75 (0.06) | 24.72 (0.03) | 24.68 (0.04) | 26.63 (0.05) | 24.57 (0.06) | 24.53 (0.04) | 24.47 (0.06) | 24.40 (0.07) | 24.35 (0.05) | 24.29 (0.06) | 24.20 (0.09) | 24.15 (0.05) | 0.94 |
EDTA 125 ppm | 33.73 | 33.67 (0.06) | 33.62 (0.05) | 33.58 (0.04) | 33.54 (0.04) | 33.48 (0.06) | 33.44 (0.04) | 33.38 (0.06) | 33.31 (0.07) | 33.29 (0.02) | 33.23 (0.06) | 33.17 (0.06) | 33.12 (0.05) | 33.07 (0.04) | 33.05 (0.03) | 33.00 (0.05) | 32.90 (0.10) | 32.83 (0.07) | 32.74 (0.09) | 0.99 |
EDTA 200 ppm | 26.47 | 26.39 (0.08) | 26.34 (0.05) | 26.30 (0.04) | 26.24 (0.06) | 26.20 (0.04) | 26.14 (0.06) | 26.09 (0.05) | 26.02 (0.07) | 25.99 (0.03) | 25.92 (0.07) | 25.84 (0.08) | 25.79 (0.03) | 25.73 (0.06) | 25.68 (0.05) | 25.64 (0.04) | 25.50 (0.14) | 25.44 (0.06) | 25.35 (0.09) | 1.12 |
KMS 500 ppm | 32.25 | 32.14 (0.11) | 32.07 (0.07) | 32.01 (0.06) | 31.94 (0.07) | 31.89 (0.05) | 31.83 (0.06) | 31.76 (0.07) | 31.72 (0.04) | 31.67 (0.05) | 31.61 (0.06) | 31.54 (0.07) | 31.48 (0.06) | 31.42 (0.06) | 31.37 (0.05) | 31.32 (0.05) | 31.20 (0.12) | 31.11 (0.09) | 31.01 (0.10) | 1.14 |
Effect of dipping treatments on weight loss of
Treatment | Whiteness of fruiting body* | |||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Initial | 1DA | 2DA | 3DA | 4DA | 5DA | 6DA | 7DA | 8DA | 9DA | 10DA | 11DA | 12DA | 13DA | 14DA | 15DA | 16DA | 17DA | 18DA | 19DA | |
EDTA 75 ppm | 1 | 1 | 1 | 3 | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — |
EDTA 125 ppm | 1 | 1 | 1 | 3 | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — |
EDTA 200 ppm | 1 | 1 | 1 | 2 | 3 | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — |
KMS 500 ppm | 1 | 1 | 1 | 3 | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — |
EDTA 75 ppm | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 3 | 3 |
EDTA 125 ppm | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 2 | 2 | 2 | 3 |
EDTA 200 ppm | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 2 | 2 | 3 |
KMS 500 ppm | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 2 | 3 |
Effect of dipping treatments on whiteness of
1—absolute white, 2—slight dull (cream), 3—light brown and Dark brown.
Treatment | Gill opening after days of storage* | |||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Initial | 1DA | 2DA | 3DA | 4DA | 5DA | 6DA | 7DA | 8DA | 9DA | 10DA | 11DA | 12DA | 13DA | 14DA | 15DA | 16DA | 17DA | 18DA | 19DA | |
EDTA 75 ppm | 1 | 2 | 5 | 5 | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — |
EDTA 125 ppm | 1 | 5 | 5 | 5 | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — |
EDTA 200 ppm | 1 | 1 | 5 | 5 | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — |
KMS 500 ppm | 1 | 3 | 5 | 5 | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — |
EDTA 75 ppm | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 4 | 4 | 4 | 5 |
EDTA 125 ppm | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 3 | 4 | 4 | 5 | — | — | — | — | — | — | — | — |
EDTA 200 ppm | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 5 | 5 | — | — | — | — | — | — | — | — | — | — | — |
KMS 500 ppm | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 3 | 3 | 3 | 3 | 3 | 4 | 4 | 4 | 4 | 5 | — | — |
Effect of dipping treatments on gill opening of
1—without opening, 2—1/4 opening, 3—1/2 opening, 4—3/4 opening and 5—full open.
Treatment | Texture after days of storage | |||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Initial | 1DA | 2DA | 3DA | 4DA | 5DA | 6DA | 7DA | 8DA | 9DA | 10DA | 11DA | 12DA | 13DA | 14DA | 15DA | 16DA | 17DA | 18DA | 19 DA | |
EDTA 75 ppm | 1 | 1 | 1 | 3 | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — |
EDTA 125 ppm | 1 | 1 | 1 | 3 | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — |
EDTA 200 ppm | 1 | 1 | 1 | 2 | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — |
KMS 500 ppm | 1 | 1 | 1 | 3 | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — |
EDTA 75 ppm | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 |
EDTA 125 ppm | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
EDTA 200 ppm | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 |
KMS 500 ppm | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 |
Effect of dipping treatments on texture of
1—smooth, 2—rough, 3—not acceptable.
Treatment | Condensation | |||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Initial | 1DA | 2DA | 3DA | 4DA | 5DA | 6DA | 7DA | 8DA | 9DA | 10DA | 11DA | 12DA | 13DA | 14DA | 15DA | 16DA | 17DA | 18DA | 19DA | |
EDTA 75 ppm | 1 | 2 | 2 | 2 | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — |
EDTA 125 ppm | 1 | 2 | 2 | 2 | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — |
EDTA 200 ppm | 1 | 2 | 3 | 3 | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — |
KMS 500 ppm | 1 | 2 | 3 | 3 | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — |
EDTA 75 ppm | 1 | 1 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 |
EDTA 125 ppm | 1 | 1 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 |
EDTA 200 ppm | 1 | 1 | 2 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 |
KMS 500 ppm | 1 | 1 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 |
Effect of dipping treatments on condensation of
1—1/4 of pp. bag, 2—1/2 of pp. bag, 3—3/4 of pp. bag and 4—full of pp. bag.
Effect of dipping treatment with different concentration of EDTA (75–200 ppm) and KMS (500 ppm) was studied on quality parameters of
Treatment | Whiteness of fruiting bodies days after storage | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Initial | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | |
EDTA 75 ppm | 1 | 1 | 1 | 1 | 1 | 2 | 4 | — | — | — | — | — | — | — | — | — | — | — | — | — | — |
EDTA 125 ppm | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 3 | 4 | — | — | — | — | — | — | — | — | — | — | — | — |
EDTA 200 ppm | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 2 | 3 | 4 | — | — | — | — | — | — | — | — | — | — |
KMS 500 ppm | 1 | 1 | 1 | 2 | 3 | 3 | 4 | — | — | — | — | — | — | — | — | — | — | — | — | — | — |
EDTA 75 ppm | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 2 |
EDTA 125 ppm | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
EDTA 200 ppm | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 2 | 3 | 3 | 3 | 4 | — | — | — | — | — | — |
KMS 500 ppm | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 2 |
Effect of dipping treatments on whiteness of
Treatment | Texture after days of storage | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Initial | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | |
EDTA 75 ppm | 1 | 1 | 1 | 1 | 1 | 1 | 1 | — | — | — | — | — | — | — | — | — | — | — | — | — | — |
EDTA 125 ppm | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | — | — | — | — | — | — | — | — | — | — | — | — | — |
EDTA 200 ppm | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | — | — | — | — | — | — | — | — | — | — | — |
KMS 500 ppm | 1 | 1 | 2 | 2 | 2 | 2 | 2 | — | — | — | — | — | — | — | — | — | — | — | — | — | — |
EDTA 75 ppm | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
EDTA 125 ppm | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
EDTA 200 ppm | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 3 | 4 | 4 | 4 | 5 | — | — | — | — | — |
KMS 500 ppm | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
Effect of dipping treatments on texture of
Treatment | Condensation days after storage | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Initial | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | |
EDTA 75 ppm | 1 | 1 | 1 | 2 | 2 | 2 | 3 | — | — | — | — | — | — | — | — | — | — | — | — | — | — |
EDTA 125 ppm | 1 | 1 | 1 | 2 | 2 | 3 | 3 | 3 | — | — | — | — | — | — | — | — | — | — | — | — | — |
EDTA 200 ppm | 1 | 1 | 1 | 2 | 2 | 3 | 3 | 3 | 3 | 3 | — | — | — | — | — | — | — | — | — | — | — |
KMS 500 ppm | 1 | 1 | 1 | 2 | 2 | 2 | 2 | — | — | — | — | — | — | — | — | — | — | — | — | — | — |
EDTA 75 ppm | 1 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 |
EDTA 125 ppm | 1 | 2 | 2 | 2 | 2 | 2 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 |
EDTA 200 ppm | 1 | 1 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | — | — | — | — | — | — | — | — | — | — | — | — |
KMS 500 ppm | 1 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 |
Effect of dipping treatments on condensation of
Treatment | Gill opening days after storage | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Initial | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | |
EDTA 75 ppm | 1 | 1 | 1 | 1 | 1 | 1 | 1 | — | — | — | — | — | — | — | — | — | — | — | — | — | — |
EDTA 125 ppm | 1 | 1 | 1 | 1 | 2 | 2 | 3 | 5 | — | — | — | — | — | — | — | — | — | — | — | — | — |
EDTA 200 ppm | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | — | — | — | — | — | — | — | — | — | — |
KMS 500 ppm | 1 | 1 | 1 | 1 | 1 | 1 | 1 | — | — | — | — | — | — | — | — | — | — | — | — | — | — |
EDTA 75 ppm | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 2 |
EDTA 125 ppm | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 3 | 4 | 5 | — | — | — | — | — | — |
EDTA 200 ppm | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
KMS 500 ppm | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
Effect of dipping treatments on gill opening of
Effect of packaging thickness on quality parameters of two button mushroom cv. S 649 and cv. NCS 100 was studied under ambient temperature and refrigerated conditions at Mushroom Research Laboratory of the Department of Plant Pathology, IGKV, Raipur. Transparent polyethylene bags of three thickness (75,100 and 125 gauge) along with one control (100 PE) was used. The fruiting bodies of 25 ± 2 cm or available size fruit bodies of cv. S 649 of
The effect of packing thickness on weight loss, veil opening and color of the fruit bodies of cv. S 649 of
S. no. | Treatments | At ambient temperature | |||||||
---|---|---|---|---|---|---|---|---|---|
Weight (% weight loss) | Veil opening | Color | |||||||
Initial | After 24 h | Initial | After 24 h | After 48 h | Initial | After 24 h | After 48 h | ||
1 | 75 Gauge | 34.63 | 34.50 (0.37) | + | ++ | +++ | + | +++ | ++++ |
2 | 100 Gauge | 39.16 | 39.08 (0.20) | + | ++ | +++ | + | +++ | ++++ |
3 | 125 Gauge | 45.00 | 44.61 (0.86) | + | ++ | +++ | + | +++ | ++++ |
4 | 100 PE (control) | 38.56 | 38.29 (0.70) | + | ++ | +++ | + | +++ | ++++ |
Packaging for button mushroom cv. S 649 (washed 0.05% KMS).
For color −+: absolute white, ++: dull white, +++: dull yellow, ++++: not acceptable.
For veil opening – +: intact fruit body, ++: veil opening up to 25%, +++: veil opening up to 50%, ++++: veil opening up to 100%.
At refrigerated temperature conditions, maximum weight loss (6.78%) in fruit bodies of strain cv. S 649 was recorded in 75 gauge pp. bags and minimum weight loss (2.72%) was noticed in 125 gauge after 5 days of storage (Table 13). In general, weight loss in fruiting bodies of
S. no. | Treatments | At refrigerated temperature | |||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Weight (% weight loss) after hours | Color after hours | Veil opening after hours | |||||||||||||||||
Initial | 24 | 48 | 72 | 96 | 120 | Initial | 24 | 48 | 72 | 96 | 120 | Initial | 24 | 48 | 72 | 96 | 120 | ||
1 | 75 Gauge PP | 34.50 | 33.26 (2.50) | 33.06 (4.17) | 32.80 (4.92) | 32.63 (5.42) | 32.16 (6.78) | + | + | ++ | ++ | ++ | ++ | + | + | ++ | ++ | ++ | ++ |
2 | 100 Gauge PP | 38.00 | 37.81 (0.50) | 37.63 (0.97) | 37.19 (2.13) | 36.98 (2.92) | 36.78 (3.21) | + | + | + | ++ | ++ | ++ | + | + | ++ | ++ | ++ | +++ |
3 | 125 Gauge PP | 51.40 | 51.08 (0.62) | 50.75 (1.26) | 50.38 (1.98) | 50.13 (2.47) | 50.00 (2.72) | + | + | ++ | ++ | ++ | ++ | + | + | ++ | ++ | ++ | ++ |
4 | 100 PE (control) | 38.63 | 38.50 (0.33) | 38.10 (1.37) | 37.76 (2.52) | 37.18 (3.75) | 36.93 (4.40) | + | + | ++ | ++ | ++ | ++ | + | ++ | ++ | ++ | +++ | +++ |
Packaging for button mushroom (
For color −+: absolute white, ++: dull white, +++: dull yellow, ++++: not acceptable.
For veil opening – +: intact fruit body, ++: veil opening up to 25%, +++: veil opening up to 50%, ++++: veil opening up to 100%.
Effect of packaging thickness on weight loss, color and veil opening of the fruiting bodies of strain cv. NCS 100 of
Treatments | At room temperature | ||||||||
---|---|---|---|---|---|---|---|---|---|
Weight (% weight loss) | Color | Veil Opening | |||||||
Initial | After 24 h | After 48 h | Initial | After 24 h | After 48 h | Initial | After 24 h | After 48 h | |
75 Gauge | 33.4 | 32.89 (1.52) | 32.54 (2.57) | + | + | ++++ | + | + | ++ |
100 Gauge | 44.77 | 43.91 (1.92) | 43.29 (3.30) | + | + | ++++ | + | + | ++ |
125 Gauge | 39.36 | 38.89 (1.19) | 38.55 (2.05) | + | + | ++++ | + | + | +++ |
100 PE (control) | 41.8 | 41.33 (1.12) | 41.00 (1.91) | + | + | ++++ | + | + | +++ |
Packaging for button mushroom cv. NCS 100 washed with KMS (0.05%).
For color: +: absolute white, ++: dull white, +++: dull yellow, ++++: not acceptable.
For veil opening: +: intact fruit body, ++: veil opening up to 25%, +++: veil opening up to 50%, ++++: veil opening up to 100%.
At refrigerated temperature, maximum weight loss (1.39%) in cv. NCS 100 was recorded in 75 gauge pp. bags and minimum weight loss (1.05%) was noticed in control after 5 days of storage (Table 15) which was remarkably less compared to the fruit bodies of cv. S 649. The fruit bodies of cv. NCS 100 of
Treatments | At refrigerated temperature | |||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Weight (% weight loss) | Color | Veil opening | ||||||||||||||||
Initial | After 24 h. | After 48 h. | After 72 h. | After 96 h. | After 120 h. | Initial | After 24 h. | After 48 h. | After 72 h. | After 96 h. | After 120 h. | Initial | After 24 h. | After 48 h. | After 72 h. | After 96 h. | After 120 h | |
75 Gauge | 37.35 | 37.26 (0.24) | 37.19 (0.42) | 37.07 (0.74) | 37.01 (0.91) | 36.83 (1.39) | + | + | + | ++ | ++ | +++ | + | + | + | + | + | + |
100 Gauge | 36.76 | 36.69 (0.19) | 36.63 (0.35) | 36.48 (0.76) | 36.40 (01.97) | 36.29 (1.27) | + | + | + | ++ | ++ | +++ | + | + | + | + | + | + |
125 Gauge | 37.8 | 37.73 (0.18) | 37.67 (0.34) | 37.60 (0.52) | 37.44 (0.84) | 37.3 (1.32) | + | + | + | + | + | ++ | + | + | + | + | + | + |
100 PE (control) | 36.91 | 36.86 (0.13) | 36.78 (0..35) | 36.72 (0.51) | 36.63 (0.75) | 36.52 (1.05) | + | + | + | ++ | ++ | +++ | + | + | + | + | + | + |
Packaging for button mushroom (
For color: +: absolute white, ++: dull white, +++: dull yellow, ++++: not acceptable.
For veil opening : +: intact fruit body, ++: veil opening up to 25%, +++: veil opening up to 50%, ++++: veil opening up to 100%.
Drying is one of most broadly-practiced and oldest methods for preserving agricultural products to maintain the quality against decaying. It is done mainly in warmer areas such as the kitchen, near the stove or fireplace. These are used as heat sources and often drying is completed in the sun [26]. Three methods of oyster mushroom preservation/drying were evaluated under farmers conditions. The oyster mushroom (200 g) were first blanched at 75°C for 2 min in water. Thereafter, it was kept in two steeping solutions of 5% salt, 0.2% citric acid, 0.15% potassium meta bisulfite (KMS). In the second treatment, the same quantity of mushroom was dried using mechanical dryer at 45–50°C for 8 h. In third treatment, the same quantity of oyster mushroom was blanched and sun dried. Blanching with the use of hot water or steam is an important treatment applied after washing to inhibit tissue browning by inactivation of polyphenol oxidase and production of off flavors. It also removes trapped air and decreases weight losses to induce mushroom shrinkage [27]. These were then observed for color, texture and overall acceptability. In another experiment, freshly harvested fruiting body (500 g) of oyster mushroom (
S. no. | Treatments | Weight gain /loss | Change in Color | Brittle ness* | Quality parameters (3 months)** | |
---|---|---|---|---|---|---|
Initial | After 3 months (% wt. loss) | |||||
1. | Sun drying with blanching (0.2% salt +0.1% CA for 2 min) | 29.63 | 29.0 (2.12) | Blackish | + | ++ |
2. | Sun drying without blanching (0.2% salt +0.1% CA for 2 min) | 37.07 | 36.03 (2.80) | Light Brown | ++ | + |
3. | Cabinet drying with blanching | 33.11 | 32.75 (1.08) | Blackish | + | + |
4. | Cabinet drying without blanching | 32.07 | 31.44 (1.96) | Yellowish | + | + |
5. | Dipping in plain water followed by sun drying (Control) | 36.50 | 35.36 (3.12) | Light Brown | ++ | + |
Studies on methods of drying of oyster mushroom (
+ Brittle, ++ Soft,
Quality parameters: + Pleasant flavor ++ Off flavor.
NB. There was no rottage and insect attack within the storage period of 3 months.
Of three treatments assessed, the fresh oyster mushroom steeped in solutions of above chemicals with or without blanching were of good quality for the period of 105 days without much loss in color, texture and acceptability. Mushroom dried in a mechanical dryer at 45–50°C for 8 h. With blanching though changed in to blackish color but the loss in weight, brittleness and quality of the fruiting body remained least influenced followed by without blanching which were of excellent quality even up to 3 months of storage. The sun dried product when dipped in plain water and kept in pp. bags after 55 days at ambient temperature lost maximum weight of the fruiting body (3.12%) than any other treatment. The fruiting body became soft and developed brown color. Thus, it can be said that the oyster mushroom steeped in solutions of above chemicals and mechanically dried can be very well preserved for the period of 105 days without much influence on quality parameters. The increase in the drying temperature though helped to accelerate the drying rate but, high temperatures (70°C) are not generally recommended because, it causes browning in the samples and deteriorates the quality which are important from customer’s viewpoint [28]. In another experiment conducted at AICMIP, Raipur center on different methods of drying of oyster mushroom exhibited drying by cabinet dryer with and without blanching to be excellent as there was minimum reduction in weight, color was retained, fruit body was brittle and pleasant flavor was noticed within the period of 3 months of storage (Table 16). Other methods of drying were not that much effective as the per cent reduction in weight was more, fruit body started turning yellowish in color, became soft and developed off flavor. The results obtained on the preservation method had significant effects on the nutrient and mineral compositions of the mushroom samples [29]. In contrast to present investigation, the lowest weight values were obtained from the sundried mushroom samples while the highest value was obtained from the fresh samples by Jonathan et al. [30] which is difficult to be explained.
In order to study the quality and storage, the sporophores of
Treatment | **Color of sporophores in 1–5 scale at different time intervals (days) | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Number | Treatment details* | 1 | 5 | 25 | 50 | 75 | 100 | 125 | 150 | 175 |
T-1 | 5% salt, 0.2% C.A., 0.1% KMS (WOB) | 1 | 4 | 5 | ||||||
T-2 | 5% salt, 0.2% C.A., 0.1% KMS (WB) | 1 | 3 | 5 | ||||||
T-3 | 2% salt, 1% sugar, 0.3% C.A., 0.1% KMS (WB) | 1 | 3 | 5 | ||||||
T-4 | 2% salt, 1% sugar, 0.3% C.A., 0.1% KMS (WOB) | 1 | 3 | 5 | ||||||
T-5 | 2.5% salt, 0.1% A.A., 0.2% C.A.,0.1% S.B. 0.1% KMS (WB) | 1 | 1 | 1 | 1 | 2 | 2 | 2 | 3 | 3 |
T-6 | 2.5% salt, 0.1% A.A., 0.2% C.A., 0.1% S.B. 0.1% KMS (WOB) | 1 | 3 | 5 | ||||||
T-7 | 0.2% A.A., 0.2% C.A., 0.2% KMS (WB) | 1 | 1 | 1 | 1 | 2 | 2 | 2 | 3 | 3 |
T-8 | 0.2% A.A., 0.2% C.A., 0.2% KMS (WOB) | 1 | 3 | 5 | ||||||
T-9 | 0.5% C.A. (WB) | 1 | 4 | 5 | ||||||
T-10 | Simple boiled water (WB) | 1 | 4 | 5 | ||||||
T-11 | 0.1% A.A., 0.2% P.A., 0.1% KMS (WB) | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 3 | |
T-12 | 5% salt, 0.2% C.A., 0.1% KMS (WB) | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 3 |
T-13 | 0.1% A.A., 0.3% C.A., 0.1% KMS, 1% ASA (WB) | 1 | 2 | 3 | 4 | |||||
T-14 | 1% salt, 0.1% A.A., 0.1% C.A., 0.05% S.B. 0.05% KMS (WB) | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 3 |
T-15 | 0.1% A.A., 0.1% C.A., 0.1% KMS (WB) | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 3 | |
T-16 | 1% Salt, 0.1% C.A., 0.05% KMS (WB) | 1 | 3 | 4 | ||||||
T-17 | 0.1% KMS, 0.2%, A.A. (WB) | 1 | 2 | 4 | ||||||
T-18 | 0. 3% A.A. (W.B.) | 2 | 4 | 5 | ||||||
T-19 | 0.2% KMS (WB) | 2 | 4 | 5 |
Effect of different steeping solution on color and storage of the sporophores of
SB—sodium benzoate, ASA—ascorbic acid, C.A.—citric acid, KMS—potassium metabisulphide.
Scale white—1, like white—2, slight dull—3, A.A.—acitic acid, P.A.—propionic acid, W.B.—with blanch, WOB—without blanch, light brown—4, dark brown—5.
Treatment | **Texture of sporophoresin 1–7 scale at different time intervals (days) | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Number | Treatment details* | 1 | 5 | 25 | 50 | 75 | 100 | 125 | 150 | 175 |
T-1 | 5% salt, 0.2% C.A., 0.1% KMS (WOB) | 1 | 7 | |||||||
T-2 | 5% salt, 0.2% C.A., 0.1% KMS (WB) | 1 | 5 | |||||||
T-3 | 2% salt, 1% sugar, 0.3% C.A., 0.1% KMS (WB) | 1 | 5 | |||||||
T-4 | 2% salt, 1% sugar, 0.3% C.A., 0.1% KMS (WOB) | 1 | 5 | |||||||
T-5 | 2.5% salt, 0.1% A.A., 0.2% C.A.,0.1% S.B. 0.1% KMS (WB) | 1 | 1 | 2 | 2 | 2 | 2 | 2 | 3 | 3 |
T-6 | 2.5% salt, 0.1% A.A., 0.2% C.A., 0.1% S.B. 0.1% KMS (WOB) | 1 | 4 | |||||||
T-7 | 0.2% A.A., 0.2% C.A., 0.2% KMS (WB) | 1 | 1 | 2 | 2 | 2 | 2 | 2 | 3 | 3 |
T-8 | 0.2% A.A., 0.2% C.A., 0.2% KMS (WOB) | 1 | 4 | |||||||
T-9 | 0.5% C.A. (WB) | 1 | 7 | |||||||
T-10 | Simple boiled water (WB) | 1 | 7 | |||||||
T-11 | 0.1% A.A., 0.2% P.A., 0.1% KMS (WB) | 1 | 2 | 2 | 2 | 2 | 2 | 3 | 3 | |
T-12 | 5% Salt, 0.2% C.A., 0.1% KMS (WB) | 1 | 2 | 2 | 2 | 2 | 2 | 2 | 3 | 3 |
T-13 | 0.1% A.A., 0.3% C.A., 0.1% KMS, 1% ASA (WB) | 1 | 2 | 2 | 4 | |||||
T-14 | 1% salt, 0.1% A.A., 0.1% C.A., 0.05% S.B. 0.05% KMS (WB) | 1 | 2 | 2 | 2 | 2 | 2 | 2 | 3 | 3 |
T-15 | 0.1% A.A., 0.1% C.A., 0.1% KMS (WB) | 1 | 2 | 2 | 2 | 2 | 2 | 3 | 3 | |
T-16 | 1% salt, 0.1% C.A., 0.05% KMS (WB) | 1 | 5 | |||||||
T-17 | 0.1% KMS, 0.2%, A.A. (WB) | 1 | 2 | 4 | ||||||
T-18 | 0. 3% A.A. (W.B.) | 1 | 7 | |||||||
T-19 | 0.2% KMS (WB) | 1 | 7 |
Effect of different steeping solution on texture and storage of the sporophores of
SB—sodium benzoate, ASA—ascorbic acid, C.A.—citric acid, KMS—potassium metabisulphide.
Scale fresh—1, like fresh—2, less sogy—3, more sogy—4, A.A.—acetic acid, P.A—propionic acid, W.B—with blanch, WOB—without blanch, coarse—5, rotting—6, leathery—7.
Treatment | **Appearance of sporophores in 1–6 scale at different time intervals(days) | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Number | Treatment details* | 1 | 5 | 25 | 50 | 75 | 100 | 125 | 150 | 175 |
T-1 | 5% salt, 0.2% C.A., 0.1% KMS (WOB) | 2 | 6 | |||||||
T-2 | 5% salt, 0.2% C.A., 0.1% KMS (WB) | 2 | 4 | |||||||
T-3 | 2% salt, 1% sugar, 0.3% C.A., 0.1% KMS (WB) | 2 | 4 | |||||||
T-4 | 2% salt, 1% sugar, 0.3% C.A., 0.1% KMS (WOB) | 2 | 4 | |||||||
T-5 | 2.5% salt, 0.1% A.A., 0.2% C.A., 0.1% S.B. 0.1% KMS (WB) | 1 | 2 | 2 | 2 | 2 | 2 | 3 | 3 | 3 |
T-6 | 2.5% salt, 0.1% A.A., 0.2% C.A., 0.1% S.B. 0.1% KMS (WOB) | 2 | 4 | |||||||
T-7 | 0.2% A.A., 0.2% C.A., 0.2% KMS (WB) | 1 | 2 | 2 | 2 | 2 | 2 | 3 | 3 | 3 |
T-8 | 0.2% A.A., 0.2% C.A., 0.2% KMS (WOB) | 2 | 4 | |||||||
T-9 | 0.5% C.A. (WB) | 2 | 6 | |||||||
T-10 | Simple boiled water (WB) | 2 | 6 | |||||||
T-11 | 0.1% A.A., 0.2% P.A., 0.1% KMS (WB) | 1 | 2 | 2 | 2 | 2 | 2 | 3 | 3 | |
T-12 | 5% salt, 0.2% C.A., 0.1% KMS (WB) | 1 | 2 | 2 | 2 | 2 | 2 | 3 | 3 | 3 |
T-13 | 0.1% A.A., 0.3% C.A., 0.1% KMS, 1% ASA (WB) | 1 | 2 | 2 | 6 | |||||
T-14 | 1% salt, 0.1% A.A., 0.1% C.A., 0.05% S.B. 0.05% KMS (WB) | 1 | 2 | 2 | 2 | 2 | 2 | 3 | 3 | 3 |
T-15 | 0.1% A.A., 0.1% C.A., 0.1% KMS (WB) | 1 | 2 | 2 | 2 | 2 | 2 | 3 | 3 | |
T-16 | 1% Salt, 0.1% C.A., 0.05% KMS (WB) | 2 | 4 | |||||||
T-17 | 0.1% KMS, 0.2%, A.A. (WB) | 2 | 2 | 6 | ||||||
T-18 | 0. 3% A.A. (W.B.) | 2 | 6 | |||||||
T-19 | 0.2% KMS (WB) | 2 | 6 |
Effect of different steeping solution on appearance and storage of the sporophores of
SB—sodium benzoate, ASA—ascorbic acid, C.A.—citric acid, KMS—potassium metabisulphide,
Scale fresh—1, very good—2, good—3, fair—4, A.A.—acitic acid, P.A—propionic acid, W.B—with blanch, WOB—without blanch, slight fermented smell—5, unacceptable—6.
Treatments | Treatment details* | Storage period of quality sporophores (days) |
---|---|---|
T-1 | 5% salt, 0.2% C.A., 0.1% KMS (WOB) | 3–4 |
T-2 | 5% salt, 0.2% C.A., 0.1% KMS (WB) | 4–5 |
T-3 | 2% salt, 1% sugar, 0.3% C.A., 0.1% KMS (WB) | 4–5 |
T-4 | 2% salt, 1% sugar, 0.3% C.A., 0.1% KMS (WOB) | 4–5 |
T-5 | 2.5% salt, 0.1% A.A., 0.2% C.A.,0.1% S.B.0.1% KMS (WB) | 165–175 |
T-6 | 2.5% salt, 0.1% A.A., 0.2% C.A., 0.1% S.B. 0.1% KMS (WOB) | 5–6 |
T-7 | 0.2% A.A., 0.2% C.A., 0.2% KMS (WB) | 165–175 |
T-8 | 0.2% A.A., 0.2% C.A., 0.2% KMS (WOB) | 5–6 |
T-9 | 0.5% C.A. (WB) | 2–3 |
T-10 | Simple boiled water (WB) | 2 |
T-11 | 0.1% A.A., 0.2%P.A., 0.1% KMS (WB) | 150–155 |
T-12 | 5% Salt, 0.2% C.A., 0.1% KMS (WB) | 165–175 |
T-13 | 0.1% A.A., 0.3% C.A., 0.1% KMS, 1% ASA (WB) | 45–48 |
T-14 | 1% salt, 0.1% A.A., 0.1% C.A., 0.05% S.B. 0.05% KMS (WB) | 165–175 |
T-15 | 0.1% A.A., 0.1% C.A., 0.1% KMS (WB) | 150–155 |
T-16 | 1% Salt, 0.1% C.A., 0.05% KMS (WB) | 5–6 |
T-17 | 0.1% KMS, 0.2%, A.A. (WB) | 20–21 |
T-18 | 0. 3% A.A. (W.B.) | 2 |
T-19 | 0.2% KMS (WB) | 2 |
Effect of steeping solution on storage and quality of sporophores of
SB—sodium benzoate, ASA—ascorbic acid, C.A.—citric acid, KMS—potassium metabisulphide. A.A.—acetic acid, P.A—propionic acid, W.B—with blanch, WOB—without blanch.
The observations of the study indicated that the treatments, T12 and T14 can retain good color (2) of the sporophores of
The texture of the sporophores preserved in steeping solutions of T12 and T14 was almost fresh (2) up to 125 days and was acceptable (3) up to 175 days (Table 18). In T11 and T15, the sporophores were like fresh (2) till 100 days and up to the acceptable period of 150 days. In rest of the treatments, the sporophores preserved in steeping solutions of different chemicals showed more sogginess (4), rotted (6) leathery (7) and became unacceptable for consumption within 5 to 25 days. In T2, T9, T10, T18, T19, the sporophores of
Sporophores of
It is evident from Table 20 that T5, T7, T12 and T14 preserved the mushroom (
Mushroom preservation was reported to be of great importance due to off-season household consumption among geographically spread groups of the society [31]. The shelf life of
The rural women were very well aware with the naturally growing edible mushrooms which they do collect during monsoon season. In the villages, it is a common practice to dehydrate naturally growing mushroom in a local Bhatti and use it in a season when it is not available. With this background, the interested women were selected for training programme on Mushroom Processing Technology. Before selection, they were interviewed for necessity of such training programs. They emphasized that the fresh mushroom is not being sold many a times and it becomes difficult for them to preserve it. Oyster mushroom which were cultivated by them was sun dried as per our directives.
Seventy-six women from Tarra, Dondekhurd and Matia villages were selected for the training on Mushroom Processing Technology. For establishment of processing units at Tarra and Dondekhurd, solar dryers and mechanical tray dryers for drying of mushrooms were procured which are used by the women for drying of edible mushrooms. Oyster mushroom after dehydration was grind into powder, sieved and added @ 10–25% in preparation of various mushroom processed products. Mushroom powder was added in preparation of some of the local products like murku, bijoura, chakli etc. by Thakur [14, 15]. The training programs on Mushroom Processing Technology was mainly imparted to the women in preparation of Mushroom Mung Papad, Mushroom Urd Papad, Steamed Rice Mushroom Papad, Mushroom Badi, Mushroom Soup, Mushroom Bijora, Mushroom Murku, Mushroom Chakli, Mushroom Biscuits, Mushroom Pickles. The women of all the villages very much liked the training on this aspect. The mushroom dishes prepared by the women were highly appreciated during Peer Review Team. They were willing to sale the processed mushroom products in the local market. Mushroom pickle, mushroom badi and mushroom papad became very much popular among the women and they see very good future of these products in Chhattisgarh Market.
An attempt was made to establish the linkages with the mushroom entrepreneurs working in different parts of Chhattisgarh. The persons involved in this business were called at the villages, samples were given to them and they assured the disposal of their fresh and processed mushroom products. It has also been tried to display their products in Business Counseling Centers to be established by Swa Shakti Project in the rural areas.
It was found that the button mushrooms and their varieties when steeped in solutions of different chemicals (EDTA and KMS) and packaging materials (thickness) were able to sufficiently enhance the shelf life under both ambient and refrigerated conditions. Similarly, drying methods particularly cabinet drying at 45–60°C with blanching or without blanching did play an important role in extension of shelf life of oyster mushroom for 3 months under ambient conditions without much influence on quality parameters. Oyster mushroom when preserved in different chemical solutions of lower concentrations, it was found to prolong the shelf life of fresh oyster mushroom up to 6 months without much influencing the quality parameters. Similarly, the shelf life of dried oyster mushroom was also enhanced for 105 days even preserving at ambient temperature conditions. Processing of mushroom and their value addition in preparing various local or traditional dishes incorporating mushrooms had a great role to play in extending the shelf life and minimizing the existing problem of under nourishment and mal nourishment prevailing in several states of India particularly in weaker section of the society. Thus, there is an urgent need to promote mushroom processed products like royal oyster capsule, mushroom fortified wheat flour, mushroom based mung/urd nuggets, mushroom based mug/urd papad, mushroom rice papad, mushroom instant soup, mushroom pickle, mushroom powder, mushroom soup etc. so as to popularize them among the mankind and promote marketing of these products.
IntechOpen implements a robust policy to minimize and deal with instances of fraud or misconduct. As part of our general commitment to transparency and openness, and in order to maintain high scientific standards, we have a well-defined editorial policy regarding Retractions and Corrections.
",metaTitle:"Retraction and Correction Policy",metaDescription:"Retraction and Correction Policy",metaKeywords:null,canonicalURL:"/page/retraction-and-correction-policy",contentRaw:'[{"type":"htmlEditorComponent","content":"IntechOpen’s Retraction and Correction Policy has been developed in accordance with the Committee on Publication Ethics (COPE) publication guidelines relating to scientific misconduct and research ethics:
\\n\\n1. RETRACTIONS
\\n\\nA Retraction of a Chapter will be issued by the Academic Editor, either following an Author’s request to do so or when there is a 3rd party report of scientific misconduct. Upon receipt of a report by a 3rd party, the Academic Editor will investigate any allegations of scientific misconduct, working in cooperation with the Author(s) and their institution(s).
\\n\\nA formal Retraction will be issued when there is clear and conclusive evidence of any of the following:
\\n\\nPublishing of a Retraction Notice will adhere to the following guidelines:
\\n\\n1.2. REMOVALS AND CANCELLATIONS
\\n\\n2. STATEMENTS OF CONCERN
\\n\\nA Statement of Concern detailing alleged misconduct will be issued by the Academic Editor or publisher following a 3rd party report of scientific misconduct when:
\\n\\nIntechOpen believes that the number of occasions on which a Statement of Concern is issued will be very few in number. In all cases when such a decision has been taken by the Academic Editor the decision will be reviewed by another editor to whom the author can make representations.
\\n\\n3. CORRECTIONS
\\n\\nA Correction will be issued by the Academic Editor when:
\\n\\n3.1. ERRATUM
\\n\\nAn Erratum will be issued by the Academic Editor when it is determined that a mistake in a Chapter originates from the production process handled by the publisher.
\\n\\nA published Erratum will adhere to the Retraction Notice publishing guidelines outlined above.
\\n\\n3.2. CORRIGENDUM
\\n\\nA Corrigendum will be issued by the Academic Editor when it is determined that a mistake in a Chapter is a result of an Author’s miscalculation or oversight. A published Corrigendum will adhere to the Retraction Notice publishing guidelines outlined above.
\\n\\n4. FINAL REMARKS
\\n\\nIntechOpen wishes to emphasize that the final decision on whether a Retraction, Statement of Concern, or a Correction will be issued rests with the Academic Editor. The publisher is obliged to act upon any reports of scientific misconduct in its publications and to make a reasonable effort to facilitate any subsequent investigation of such claims.
\\n\\nIn the case of Retraction or removal of the Work, the publisher will be under no obligation to refund the APC.
\\n\\nThe general principles set out above apply to Retractions and Corrections issued in all IntechOpen publications.
\\n\\nAny suggestions or comments on this Policy are welcome and may be sent to permissions@intechopen.com.
\\n\\nPolicy last updated: 2017-09-11
\\n"}]'},components:[{type:"htmlEditorComponent",content:'IntechOpen’s Retraction and Correction Policy has been developed in accordance with the Committee on Publication Ethics (COPE) publication guidelines relating to scientific misconduct and research ethics:
\n\n1. RETRACTIONS
\n\nA Retraction of a Chapter will be issued by the Academic Editor, either following an Author’s request to do so or when there is a 3rd party report of scientific misconduct. Upon receipt of a report by a 3rd party, the Academic Editor will investigate any allegations of scientific misconduct, working in cooperation with the Author(s) and their institution(s).
\n\nA formal Retraction will be issued when there is clear and conclusive evidence of any of the following:
\n\nPublishing of a Retraction Notice will adhere to the following guidelines:
\n\n1.2. REMOVALS AND CANCELLATIONS
\n\n2. STATEMENTS OF CONCERN
\n\nA Statement of Concern detailing alleged misconduct will be issued by the Academic Editor or publisher following a 3rd party report of scientific misconduct when:
\n\nIntechOpen believes that the number of occasions on which a Statement of Concern is issued will be very few in number. In all cases when such a decision has been taken by the Academic Editor the decision will be reviewed by another editor to whom the author can make representations.
\n\n3. CORRECTIONS
\n\nA Correction will be issued by the Academic Editor when:
\n\n3.1. ERRATUM
\n\nAn Erratum will be issued by the Academic Editor when it is determined that a mistake in a Chapter originates from the production process handled by the publisher.
\n\nA published Erratum will adhere to the Retraction Notice publishing guidelines outlined above.
\n\n3.2. CORRIGENDUM
\n\nA Corrigendum will be issued by the Academic Editor when it is determined that a mistake in a Chapter is a result of an Author’s miscalculation or oversight. A published Corrigendum will adhere to the Retraction Notice publishing guidelines outlined above.
\n\n4. FINAL REMARKS
\n\nIntechOpen wishes to emphasize that the final decision on whether a Retraction, Statement of Concern, or a Correction will be issued rests with the Academic Editor. The publisher is obliged to act upon any reports of scientific misconduct in its publications and to make a reasonable effort to facilitate any subsequent investigation of such claims.
\n\nIn the case of Retraction or removal of the Work, the publisher will be under no obligation to refund the APC.
\n\nThe general principles set out above apply to Retractions and Corrections issued in all IntechOpen publications.
\n\nAny suggestions or comments on this Policy are welcome and may be sent to permissions@intechopen.com.
\n\nPolicy last updated: 2017-09-11
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