\\n\\n
Released this past November, the list is based on data collected from the Web of Science and highlights some of the world’s most influential scientific minds by naming the researchers whose publications over the previous decade have included a high number of Highly Cited Papers placing them among the top 1% most-cited.
\\n\\nWe wish to congratulate all of the researchers named and especially our authors on this amazing accomplishment! We are happy and proud to share in their success!
Note: Edited in March 2021
\\n"}]',published:!0,mainMedia:{caption:"Highly Cited",originalUrl:"/media/original/117"}},components:[{type:"htmlEditorComponent",content:'IntechOpen is proud to announce that 191 of our authors have made the Clarivate™ Highly Cited Researchers List for 2020, ranking them among the top 1% most-cited.
\n\nThroughout the years, the list has named a total of 261 IntechOpen authors as Highly Cited. Of those researchers, 69 have been featured on the list multiple times.
\n\n\n\nReleased this past November, the list is based on data collected from the Web of Science and highlights some of the world’s most influential scientific minds by naming the researchers whose publications over the previous decade have included a high number of Highly Cited Papers placing them among the top 1% most-cited.
\n\nWe wish to congratulate all of the researchers named and especially our authors on this amazing accomplishment! We are happy and proud to share in their success!
Note: Edited in March 2021
\n'}],latestNews:[{slug:"webinar-introduction-to-open-science-wednesday-18-may-1-pm-cest-20220518",title:"Webinar: Introduction to Open Science | Wednesday 18 May, 1 PM CEST"},{slug:"step-in-the-right-direction-intechopen-launches-a-portfolio-of-open-science-journals-20220414",title:"Step in the Right Direction: IntechOpen Launches a Portfolio of Open Science Journals"},{slug:"let-s-meet-at-london-book-fair-5-7-april-2022-olympia-london-20220321",title:"Let’s meet at London Book Fair, 5-7 April 2022, Olympia London"},{slug:"50-books-published-as-part-of-intechopen-and-knowledge-unlatched-ku-collaboration-20220316",title:"50 Books published as part of IntechOpen and Knowledge Unlatched (KU) Collaboration"},{slug:"intechopen-joins-the-united-nations-sustainable-development-goals-publishers-compact-20221702",title:"IntechOpen joins the United Nations Sustainable Development Goals Publishers Compact"},{slug:"intechopen-signs-exclusive-representation-agreement-with-lsr-libros-servicios-y-representaciones-s-a-de-c-v-20211123",title:"IntechOpen Signs Exclusive Representation Agreement with LSR Libros Servicios y Representaciones S.A. de C.V"},{slug:"intechopen-expands-partnership-with-research4life-20211110",title:"IntechOpen Expands Partnership with Research4Life"},{slug:"introducing-intechopen-book-series-a-new-publishing-format-for-oa-books-20210915",title:"Introducing IntechOpen Book Series - A New Publishing Format for OA Books"}]},book:{item:{type:"book",id:"5066",leadTitle:null,fullTitle:"Abiotic and Biotic Stress in Plants - Recent Advances and Future Perspectives",title:"Abiotic and Biotic Stress in Plants",subtitle:"Recent Advances and Future Perspectives",reviewType:"peer-reviewed",abstract:"The impact of global climate change on crop production has emerged as a major research priority during the past decade. Understanding abiotic stress factors such as temperature and drought tolerance and biotic stress tolerance traits such as insect pest and pathogen resistance in combination with high yield in plants is of paramount importance to counter climate change related adverse effects on the productivity of crops. In this multi-authored book, we present synthesis of information for developing strategies to combat plant stress. Our effort here is to present a judicious mixture of basic as well as applied research outlooks so as to interest workers in all areas of plant science. We trust that the information covered in this book would bridge the much-researched area of stress in plants with the much-needed information for evolving climate-ready crop cultivars to ensure food security in the future.",isbn:null,printIsbn:"978-953-51-2250-0",pdfIsbn:"978-953-51-4209-6",doi:"10.5772/60477",price:159,priceEur:175,priceUsd:205,slug:"abiotic-and-biotic-stress-in-plants-recent-advances-and-future-perspectives",numberOfPages:768,isOpenForSubmission:!1,isInWos:1,isInBkci:!0,hash:"d37213a7dbc2b6704c13b7ee519b563b",bookSignature:"Arun K. Shanker and Chitra Shanker",publishedDate:"February 17th 2016",coverURL:"https://cdn.intechopen.com/books/images_new/5066.jpg",numberOfDownloads:77713,numberOfWosCitations:363,numberOfCrossrefCitations:223,numberOfCrossrefCitationsByBook:15,numberOfDimensionsCitations:511,numberOfDimensionsCitationsByBook:17,hasAltmetrics:1,numberOfTotalCitations:1097,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"March 18th 2015",dateEndSecondStepPublish:"April 8th 2015",dateEndThirdStepPublish:"July 13th 2015",dateEndFourthStepPublish:"October 11th 2015",dateEndFifthStepPublish:"March 2nd 2016",currentStepOfPublishingProcess:5,indexedIn:"1,2,3,4,5,6,8,9",editedByType:"Edited by",kuFlag:!1,featuredMarkup:null,editors:[{id:"58592",title:"Dr.",name:"Arun",middleName:null,surname:"Shanker",slug:"arun-shanker",fullName:"Arun Shanker",profilePictureURL:"https://mts.intechopen.com/storage/users/58592/images/1664_n.jpg",biography:"Arun K. Shanker is serving as a Principal Scientist (Plant Physiology) with the Indian Council of Agricultural Research (ICAR) at the Central Research Institute for Dryland Agriculture in Hyderabad, India. He is working with the ICAR as a full time researcher since 1993 and has since earned his Advanced degree in Crop Physiology while in service. He has been awarded the prestigious Member of the Royal Society of Chemistry (MRSC), by the Royal Society of Chemistry, London in 2015. Presently he is working on systems biology approach to study the mechanism of abiotic stress tolerance in crops. His main focus now is to unravel the mechanism of drought and heat stress response in plants to tackle climate change related threats in agriculture.",institutionString:null,position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"0",totalChapterViews:"0",totalEditedBooks:"4",institution:{name:"Indian Council of Agricultural Research",institutionURL:null,country:{name:"India"}}}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,coeditorOne:{id:"200434",title:"Dr.",name:"Chitra",middleName:null,surname:"Shanker",slug:"chitra-shanker",fullName:"Chitra Shanker",profilePictureURL:"https://mts.intechopen.com/storage/users/200434/images/4922_n.jpg",biography:"Chitra Shanker is a Principal Scientist (Agricultural Entomology) at the ICAR-Indian Institute of Rice Research in Hyderabad, India. She has earned her Masters and Doctoral degrees in Agricultural Entomology from Tamil Nadu Agricultural University, Coimbatore, India. Her research interests include integrated pest management and invertebrate biodiversity analysis. Her current research focuses on the analysis of invertebrate biodiversity and harnessing its potential for natural control of pests in rice ecosystem. More specifically, her work examines the impact of floral diversity and ecological engineering concepts and is well published in this field. She is also involved in the development of the Rice Knowledge Management Portal, an ICT initiative to serve improved knowledge and skill sets to a wide range of stakeholders of rice and developing web- and mobile-based farmer-friendly diagnostic tools for decision support.",institutionString:null,position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"0",totalChapterViews:"0",totalEditedBooks:"0",institution:null},coeditorTwo:null,coeditorThree:null,coeditorFour:null,coeditorFive:null,topics:[{id:"362",title:"Food Technology",slug:"agricultural-and-biological-sciences-plant-biology-food-technology"}],chapters:[{id:"49918",title:"Transcriptional Network Involved in Drought Response and Adaptation in Cereals",doi:"10.5772/62336",slug:"transcriptional-network-involved-in-drought-response-and-adaptation-in-cereals",totalDownloads:3135,totalCrossrefCites:6,totalDimensionsCites:13,hasAltmetrics:0,abstract:"Drought is the major abiotic stress in many wheat environments, decreasing grain yields and farmer’s income. Finding ways to improve drought tolerance in wheat is therefore a global effort. Transcription factors (TFs) play important roles in drought tolerance by stimulating plant’s protective genome activities in response to heat and water limitation. TFs are specialized proteins which can bind to specific DNA elements in gene promoters and modulate gene expression in response to various external and internal stimuli. Thus TFs is a crucial part of plant signal transduction pathway mediated by signal receptors, phytohormones and other regulatory compounds. The activities of TFs are closely related to their structure, and their binding specificity is determined by the homo-/hetero-dimerization of TFs. The expression of downstream genes may produce a subset of TFs or regulate other functional proteins involved in physiological drought adaptation. Thus, the hierarchic regulations of TF activities, downstream gene expression and protein–protein interaction comprise a complex regulatory network, which participates in drought response and adaptation in cereal crops. Basic mechanisms of this regulatory network have been described, but more insight is needed to find new tools for enhancing cereals’ adaptation to drought stress.",signatures:"Yunfei Yang, Pradeep Sornaraj, Nikolai Borisjuk, Nataliya Kovalchuk\nand Stephan M. Haefele",downloadPdfUrl:"/chapter/pdf-download/49918",previewPdfUrl:"/chapter/pdf-preview/49918",authors:[{id:"176288",title:"Dr.",name:"Stephan",surname:"Haefele",slug:"stephan-haefele",fullName:"Stephan Haefele"}],corrections:null},{id:"49400",title:"Histone Methylation - A Cornerstone for Plant Responses to Environmental Stresses?",doi:"10.5772/61733",slug:"histone-methylation-a-cornerstone-for-plant-responses-to-environmental-stresses-",totalDownloads:2360,totalCrossrefCites:6,totalDimensionsCites:10,hasAltmetrics:0,abstract:"Plants are sessile organisms and, as such, their survival relies on their ability to respond quickly all along their life cycle to any kind of environmental stimuli, including abiotic and biotic stresses. In this respect, plants have developed efficient mechanisms of protection and/or adaptation to minimize deleterious effects of stress on their growth and development. In a stress type-dependent manner, external signals are firstly sensed. This step is then followed by the activation of particular signalling pathways, resulting ultimately in the rapid and specific modulation of the plant transcriptome. Currently, transcriptional regulation is considered as a central process in the build-up of plant responses to both abiotic and biotic stresses. Among mechanisms involved in transcriptional regulation, the combined effect of different histone tail post-translational modifications (PTMs; e.g. acetylation and methylation) through the activity of particular histone-modifying enzymes can lead to changes in the local chromatin structure environment and hence the underlying DNA accessibility.",signatures:"Roberto Bobadilla and Alexandre Berr",downloadPdfUrl:"/chapter/pdf-download/49400",previewPdfUrl:"/chapter/pdf-preview/49400",authors:[{id:"176404",title:"Dr.",name:"Alexandre",surname:"Berr",slug:"alexandre-berr",fullName:"Alexandre Berr"}],corrections:null},{id:"49605",title:"Small RNAs in Plant Response to Abiotic Stress",doi:"10.5772/61834",slug:"small-rnas-in-plant-response-to-abiotic-stress",totalDownloads:2244,totalCrossrefCites:2,totalDimensionsCites:6,hasAltmetrics:0,abstract:"Small noncoding (nc) RNAs (sRNAs) are the important posttranscriptional regulatory factors in gene regulatory networks. They are involved in many important processes of plant development and stress responses. Increasingly research data reveal that microRNAs (miRNAs), heterochromatic small interfering RNAs (hc-siRNAs), trans-acting small interfering RNAs (ta-siRNAs), natural antisense small interfering RNAs (nat-siRNAs), repeat-associated small interfering RNAs (ra-siRNAs), and the piwi-interacting RNAs (piRNAs) are involved in heat stress, salt stress, cold stress, and drought stress, which are found in metazoans. Some small RNAs are required for plant thermotolerance and salt tolerance. These findings facilitate our investigation of the genetic basis of plant adaptability to various environmental stresses and the genetic manipulation of plant tolerance to many abiotic stresses. This chapter highlights the recent advances in understanding the crucial roles of sRNAs in plant responses to heat, drought, salinity, and cold and proposes the potential technologies and strategies used to identify abiotic-stress-regulated sRNAs in addition to the recent advances and methods for validation and analysis of their target genes.",signatures:"By Shirin Mirlohi and Yuke He",downloadPdfUrl:"/chapter/pdf-download/49605",previewPdfUrl:"/chapter/pdf-preview/49605",authors:[{id:"176413",title:"Prof.",name:"Yuke",surname:"He",slug:"yuke-he",fullName:"Yuke He"},{id:"177623",title:"Ms.",name:"Shirin",surname:"Mirlohi",slug:"shirin-mirlohi",fullName:"Shirin Mirlohi"}],corrections:null},{id:"49279",title:"The Transcriptional Modulation of Inositols and Raffinose Family Oligosaccharides Pathways in Plants — An (A)Biotic Stress Perspective",doi:"10.5772/61341",slug:"the-transcriptional-modulation-of-inositols-and-raffinose-family-oligosaccharides-pathways-in-plants",totalDownloads:2010,totalCrossrefCites:0,totalDimensionsCites:1,hasAltmetrics:0,abstract:"Among the multifunctional molecules that participate in processes of plant tolerance/resistance to stresses, inositol (Ins) and its derivatives (phosphorylated, methylated, oxygenated, and Raffinose Family Oligosaccharides) have attracted the attention of researchers. These compounds represent versatile and dynamic signaling molecules and osmolytes in all eukaryotes. Due to the impacts related to Ins and its derivatives in a plant cell, assays have been conducted to understand how these biomolecules affect plant physiology. Thus, overexpression or knockout of Ins-related genes has been shown as interesting strategies for generating more efficient plants capable of growing under stress conditions. In this chapter, studies using molecular tools are presented, and the impacts of their results are discussed based on the plant stress tolerance/resistance. Furthermore, an informative panel is provided with transcriptional modulation of genes related to Ins and its derivatives expressed in plants under stress. There is a gap involving about two dozen enzymes associated with the synthesis of Ins-related compounds that have not been adequately studied, and they represent an area of high biotechnological potential.",signatures:"José Ribamar Costa Ferreira Neto, Amanda Cordeiro de Melo Souza,\nManassés Daniel da Silva, Ana Maria Benko-Iseppon, Valesca\nPandolfi, Antônio Félix da Costa and Ederson Akio Kido",downloadPdfUrl:"/chapter/pdf-download/49279",previewPdfUrl:"/chapter/pdf-preview/49279",authors:[{id:"60397",title:"Prof.",name:"José Ribamar Costa",surname:"Ferreira Neto",slug:"jose-ribamar-costa-ferreira-neto",fullName:"José Ribamar Costa Ferreira Neto"},{id:"60398",title:"Dr.",name:"Valesca",surname:"Pandolfi",slug:"valesca-pandolfi",fullName:"Valesca Pandolfi"},{id:"60400",title:"MSc.",name:"Manasses",surname:"Silva",slug:"manasses-silva",fullName:"Manasses Silva"},{id:"60597",title:"Prof.",name:"Ederson",surname:"Kido",slug:"ederson-kido",fullName:"Ederson Kido"},{id:"120406",title:"Dr.",name:"Ana Maria",surname:"Benko-Iseppon",slug:"ana-maria-benko-iseppon",fullName:"Ana Maria Benko-Iseppon"},{id:"177384",title:"MSc.",name:"Amanda Cordeiro",surname:"De Melo Souza",slug:"amanda-cordeiro-de-melo-souza",fullName:"Amanda Cordeiro De Melo Souza"},{id:"177385",title:"Dr.",name:"Antônio Félix",surname:"Da Costa",slug:"antonio-felix-da-costa",fullName:"Antônio Félix Da Costa"}],corrections:null},{id:"49696",title:"Effect of Salinity Stress on Gene Expression in Black Tiger Shrimp Penaeus monodon",doi:"10.5772/61960",slug:"effect-of-salinity-stress-on-gene-expression-in-black-tiger-shrimp-penaeus-monodon",totalDownloads:2302,totalCrossrefCites:1,totalDimensionsCites:4,hasAltmetrics:0,abstract:"Penaeid shrimps are euryhaline in nature and have the ability to survive and adapt in a wide range of salinities (3–50 ppt). The shrimps are cultured under a variety of conditions in many tropical and subtropical countries. Osmotic and ionic regulation is an important mechanism of environmental adaptation in crustaceans. However, drastic changes in abiotic and biotic conditions result in stress to the shrimps during the culture period. Salinity and temperature are the two major environmental factors that have huge impact on culture shrimp, affecting their physiological and metabolic parameters, which in turn affect shrimp growth, molting, and survival. Changes in the abiotic factors, chemical and biotic factors result in reduced immunity of shrimp and vulnerability to bacterial and viral diseases. This chapter describes the effects of low and high salinity on the gene profile changes of black tiger shrimp Penaeus monodon, and the functional role of these genes in shrimp salinity stress is discussed.",signatures:"Shekhar S. Mudagandur, Gopikrishna Gopalapillay and Koyadan K.\nVijayan",downloadPdfUrl:"/chapter/pdf-download/49696",previewPdfUrl:"/chapter/pdf-preview/49696",authors:[{id:"176219",title:"Dr.",name:"KK",surname:"Vijayan",slug:"kk-vijayan",fullName:"KK Vijayan"},{id:"176261",title:"Dr.",name:"Shekhar",surname:"M.S.",slug:"shekhar-m.s.",fullName:"Shekhar M.S."},{id:"176262",title:"Dr.",name:"G.",surname:"Gopikrishna",slug:"g.-gopikrishna",fullName:"G. Gopikrishna"}],corrections:null},{id:"49750",title:"Functional Genomics of Biotic and Abiotic Stresses in Phaseolus vulgaris",doi:"10.5772/62051",slug:"functional-genomics-of-biotic-and-abiotic-stresses-in-phaseolus-vulgaris",totalDownloads:2141,totalCrossrefCites:4,totalDimensionsCites:4,hasAltmetrics:0,abstract:"Common bean is the most important legume for human consumption in the world, being a crop extremely diverse in cultivation methods, uses, range of environments in which it is adapted, morphological variety, among others. Besides its high demand and production, this crop is threatened by a series of biotic and abiotic adversities during its life cycle, which leads to losses in yield of up to 100%. In this chapter, we explored the main constraints that affect common bean and the ways this plant reaches tolerance or resistance to them, highlighting studies at the molecular level that enabled to understand the mechanisms by which common bean perceives, responds, and adapts to a stress condition. Special focus has been given to the most recent findings in the understanding of the mechanisms underlying drought tolerance and anthracnose resistance. Thereby, we reviewed some genetic and functional genomic studies concerning the genes and pathways involved in each case. Furthermore, we outline important genetic resources of Phaseolus vulgaris, as well as the technologies and methods used toward these findings.",signatures:"Danielle Gregorio Gomes Caldas, Enéas Ricardo Konzen, Gustavo\nHenrique Recchia, Ana Carolina Vieira Zakir Pereira and Siu Mui Tsai",downloadPdfUrl:"/chapter/pdf-download/49750",previewPdfUrl:"/chapter/pdf-preview/49750",authors:[{id:"90489",title:"Prof.",name:"Siu Mui",surname:"Tsai",slug:"siu-mui-tsai",fullName:"Siu Mui Tsai"},{id:"176127",title:"Dr.",name:"Danielle",surname:"Caldas",slug:"danielle-caldas",fullName:"Danielle Caldas"},{id:"176318",title:"MSc.",name:"Gustavo",surname:"Recchia",slug:"gustavo-recchia",fullName:"Gustavo Recchia"},{id:"176319",title:"MSc.",name:"Eneas",surname:"Konzen",slug:"eneas-konzen",fullName:"Eneas Konzen"},{id:"176320",title:"MSc.",name:"Ana Carolina",surname:"Vieira",slug:"ana-carolina-vieira",fullName:"Ana Carolina Vieira"}],corrections:null},{id:"49297",title:"Stress Responsive Non-protein Coding RNAs",doi:"10.5772/61347",slug:"stress-responsive-non-protein-coding-rnas",totalDownloads:2132,totalCrossrefCites:1,totalDimensionsCites:1,hasAltmetrics:0,abstract:"Environmental or growth conditions are constantly influencing the development and maturation of organisms. To ensure precise control of gene expression under these normal as well as stress conditions, organisms developed very accurate molecular regulatory mechanisms at transcriptional and posttranscriptional levels. Key roles of small noncoding RNAs (miRNAs and siRNAs) in regulation of gene expression are no longer astonishing us nor are constantly emerging new classes of non-protein coding RNAs (tRNA-derived small RNAs, snoRNA-derived small RNAs, mRNA-derived small RNAs, and long non-coding RNAs). Although there is evidence of involvement of those various classes of noncoding RNAs in response to stress conditions, in most cases, we are still lacking basic information on their targets and interplay between different regulatory pathways. In this review, we focus on “canonical” and “noncanonical” noncoding RNAs and their involvement in abiotic stress responses.",signatures:"Agata Tyczewska, Kamilla Bąkowska-Żywicka, Joanna Gracz and\nTomasz Twardowski",downloadPdfUrl:"/chapter/pdf-download/49297",previewPdfUrl:"/chapter/pdf-preview/49297",authors:[{id:"176078",title:"Prof.",name:"Tomasz",surname:"Twardowski",slug:"tomasz-twardowski",fullName:"Tomasz Twardowski"},{id:"177377",title:"Dr.",name:"Agata",surname:"Tyczewska",slug:"agata-tyczewska",fullName:"Agata Tyczewska"},{id:"177378",title:"Dr.",name:"Kamilla",surname:"Bąkowska-Żywicka",slug:"kamilla-bakowska-zywicka",fullName:"Kamilla Bąkowska-Żywicka"},{id:"177379",title:"Dr.",name:"Joanna",surname:"Gracz",slug:"joanna-gracz",fullName:"Joanna Gracz"}],corrections:null},{id:"49558",title:"Secondary Metabolism in Amaranthus spp. — A Genomic Approach to Understand Its Diversity and Responsiveness to Stress in Marginally Studied Crops with High Agronomic Potential",doi:"10.5772/61820",slug:"secondary-metabolism-in-amaranthus-spp-a-genomic-approach-to-understand-its-diversity-and-responsive",totalDownloads:2429,totalCrossrefCites:4,totalDimensionsCites:6,hasAltmetrics:0,abstract:"Cultivated amaranths are crops with an unrealized agronomical potential despite their high nutritional value and nutraceutic properties of their seeds and/ or leaves. They tolerate growing conditions unsuitable for cereals, and are tolerant to biotic aggressors. Several Amaranthus species are abundant of sources of secondary metabolites, mostly phenylpropanoids, predominantly in seeds and leaves, many of which may confer health benefits associated with their antioxidant properties. They could also act as defensive compounds against predators or pathogens. Recent biochemical and molecular approaches partly defined the mechanisms responsible for grain amaranth´s tolerance against biotic stress. However, the role played by secondary metabolites in (a)biotic stress amelioration in amaranth is practically unknown. Our group has identified several genes coding for enzymes involved in secondary metabolism pathways in A. hypochondriacus, in addition to related regulatory transcription factors. More than 50% of these genes involve the phenylpropanoid pathway. In this chapter, the role played by this pathway in (a)biotic stress amelioration in plants will be briefly reviewed, followed by an examination of its involvement in the conferral of nutraceutic properties to amaranth plants. A description of the progress obtained so far regarding the characterization of phenylpropanoid genes in grain amaranth will close this chapter.",signatures:"Paula Andrea Castrillón-Arbeláez and John Paul Délano Frier",downloadPdfUrl:"/chapter/pdf-download/49558",previewPdfUrl:"/chapter/pdf-preview/49558",authors:[{id:"65407",title:"Dr.",name:"John",surname:"Délano-Frier",slug:"john-delano-frier",fullName:"John Délano-Frier"},{id:"177468",title:"Dr.",name:"Paula Andrea",surname:"Castrillón-Arbeláez",slug:"paula-andrea-castrillon-arbelaez",fullName:"Paula Andrea Castrillón-Arbeláez"}],corrections:null},{id:"49751",title:"Tolerance of Plants to Toxicity Induced by Micronutrients",doi:"10.5772/62046",slug:"tolerance-of-plants-to-toxicity-induced-by-micronutrients",totalDownloads:2551,totalCrossrefCites:4,totalDimensionsCites:8,hasAltmetrics:0,abstract:"Micronutrient elements such as zinc, boron copper, iron, manganese, molybdenum, and chlorine are frequently responsible by the regulatory activity of the cell organelles, being nutrients that are absorbed and found in lower concentrations in plant tissues, they also contribute to supply the nutritional exigency of the plant. Study with Zea mays plants exposed to Zn toxicity + Si presented significant increases in stomatal conductance, net photosynthetic rate, transpiration rate, and water use efficiency, respectively, in comparison with treatment only with Zn. In relation to chlorophylls a, b and total and carotenoids presented non-significant increases, when compared to plants exposed to Zn toxicity. This study revealed the positive contribution of the Si on gas exchange and reduction of the negative effects provoked on chlorophylls and carotenoids in maize plants under Zn toxicity. Other results described that prolonged exposure to excessive Cu resulted in serious toxic effects on the rice seedlings. In contrast, Tre pretreatment has been shown to be beneficial in alleviating Cu toxicity, which was mainly attributed to the ability of Tre to restrict Cu uptake and accumulation to maintain Cu homeostasis, and to induce production of antioxidant and Gly enzymes to alleviate excessive Cu-triggered oxidative stress. Stress caused by the excessive supply of micronutrients to plants frequently promotes repercussions on oxidant system, inducing the overproduction of reactive oxygen species. The oxidative damage is a situation characterized by the large ROS accumulation and insufficient detoxification promoted by antioxidant enzymes, such as catalase and glutathione peroxidase. Different mechanisms have been proposed to explain the tolerance of plants to toxicity induced by micronutrients, as uses of other elements and substances, in which it can positively act with specific transporters, metal ion homeostasis and compartmentalization of micronutrients into the vacuole.",signatures:"Allan Klynger da Silva Lobato, Emily Juliane Alvino Lima, Elaine\nMaria Silva Guedes Lobato, Gabriel Mascarenhas Maciel and\nDouglas José Marques",downloadPdfUrl:"/chapter/pdf-download/49751",previewPdfUrl:"/chapter/pdf-preview/49751",authors:[{id:"79200",title:"Prof.",name:"Allan",surname:"Klynger da Silva Lobato",slug:"allan-klynger-da-silva-lobato",fullName:"Allan Klynger da Silva Lobato"},{id:"158046",title:"Dr.",name:"Elaine Maria Silva Guedes",surname:"Lobato",slug:"elaine-maria-silva-guedes-lobato",fullName:"Elaine Maria Silva Guedes Lobato"},{id:"176132",title:"Dr.",name:"Antonio",surname:"Fernandes",slug:"antonio-fernandes",fullName:"Antonio Fernandes"},{id:"176253",title:"MSc.",name:"Odyone",surname:"Silva",slug:"odyone-silva",fullName:"Odyone Silva"}],corrections:null},{id:"49289",title:"Abiotic and Biotic Elicitors–Role in Secondary Metabolites Production through In Vitro Culture of Medicinal Plants",doi:"10.5772/61442",slug:"abiotic-and-biotic-elicitors-role-in-secondary-metabolites-production-through-in-vitro-culture-of-me",totalDownloads:7007,totalCrossrefCites:40,totalDimensionsCites:109,hasAltmetrics:0,abstract:"Plant secondary metabolites are having the great application in human health and nutritional aspect. Plant cell and organ culture systems are feasible option for the production of secondary metabolites that are of commercial importance in pharmaceuticals, food additives, flavors, and other industrial materials. The stress, including various elicitors or signal molecules, often induces the secondary metabolite production in the plant tissue culture system. The recent developments in elicitation of plant tissue culture have opened a new avenue for the production of secondary metabolite compounds. Secondary metabolite synthesis and accumulation in cell and organ cultures can be triggered by the application of elicitors to the culture medium. Elicitors are the chemical compounds from abiotic and biotic sources that can stimulate stress responses in plants, leading to the enhanced synthesis and accumulation of secondary metabolites or the induction of novel secondary metabolites. Elicitor type, dose, and treatment schedule are major factors determining the effects on the secondary metabolite production. The number of parameters, such as elicitor concentrations, duration of exposure, cell line, nutrient composition, and age or stage of the culture, is also important factors influencing the successful production of biomass and secondary metabolite accumulation. This chapter reviews the various abiotic and biotic elicitors applied to cultural system and their stimulating effects on the accumulation of secondary metabolites.",signatures:"Poornananda M. Naik and Jameel M. Al–Khayri",downloadPdfUrl:"/chapter/pdf-download/49289",previewPdfUrl:"/chapter/pdf-preview/49289",authors:[{id:"176282",title:"Prof.",name:"Jameel M.",surname:"Al-Khayri",slug:"jameel-m.-al-khayri",fullName:"Jameel M. Al-Khayri"},{id:"176284",title:"Dr.",name:"Poornananda M.",surname:"Naik",slug:"poornananda-m.-naik",fullName:"Poornananda M. Naik"}],corrections:null},{id:"49883",title:"Consequences of Water Deficit on Metabolism of Legumes",doi:"10.5772/62346",slug:"consequences-of-water-deficit-on-metabolism-of-legumes",totalDownloads:1995,totalCrossrefCites:1,totalDimensionsCites:2,hasAltmetrics:0,abstract:"The aim of this chapter is (i) to define water deficit and its consequences on growth and development of higher plants; (ii) to present the interferences induced on metabolism, including gas exchange, biochemical compounds, and osmotic substances; and (iii) to explain how these alterations will affect the growth, development, and production of leguminous plants. This chapter reports that the performance in leguminous is affected by water deficiency, which can cause lower growth and development. For gas exchange, leaf relative water content, stomatal conductance, and transpiration rate suffered decrease when cultivated under water deficit. Biochemical compounds, such as soluble amino acids, soluble proteins, proline, and abscisic acid (ABA), are intensively modified after a period under water restriction. The results prove that ABA mediates actively and significantly the proline accumulation and consequent osmotic adjustment in Vigna unguiculata leaves that were induced to water deficit and rehydration.",signatures:"Allan Klynger da Silva Lobato, Gélia Dinah Monteiro Viana,\nGleberson Guillen Piccinin, Milton Hélio Lima da Silva, Gabriel\nMascarenhas Maciel and Douglas José Marques",downloadPdfUrl:"/chapter/pdf-download/49883",previewPdfUrl:"/chapter/pdf-preview/49883",authors:[{id:"79200",title:"Prof.",name:"Allan",surname:"Klynger da Silva Lobato",slug:"allan-klynger-da-silva-lobato",fullName:"Allan Klynger da Silva Lobato"},{id:"111434",title:"MSc.",name:"Gleberson",surname:"Guillen Piccinin",slug:"gleberson-guillen-piccinin",fullName:"Gleberson Guillen Piccinin"},{id:"176133",title:"MSc.",name:"Maria",surname:"Barbosa",slug:"maria-barbosa",fullName:"Maria Barbosa"},{id:"176134",title:"MSc.",name:"Luan",surname:"Silva",slug:"luan-silva",fullName:"Luan Silva"},{id:"176941",title:"MSc.",name:"Gelia",surname:"Viana",slug:"gelia-viana",fullName:"Gelia Viana"}],corrections:null},{id:"49634",title:"Glycation of Plant Proteins under Environmental Stress — Methodological Approaches, Potential Mechanisms and Biological Role",doi:"10.5772/61860",slug:"glycation-of-plant-proteins-under-environmental-stress-methodological-approaches-potential-mechanism",totalDownloads:2077,totalCrossrefCites:2,totalDimensionsCites:5,hasAltmetrics:0,abstract:"Environmental stress is one of the major factors reducing crop productivity. Due to the oncoming climate changes, the effects of drought and high light on plants play an increasing role in modern agriculture. These changes are accompanied with a progressing contamination of soils with heavy metals. Independent of their nature, environmental alterations result in development of oxidative stress, i.e. increase of reactive oxygen species (ROS) contents, and metabolic adjustment, i.e. accumulation of soluble primary metabolites (amino acids and sugars). However, a simultaneous increase of ROS and sugar concentrations ultimately results in protein glycation, i.e. non-enzymatic interaction of reducing sugars or their degradation products (α-dicarbonyls) with proteins. The eventually resulting advanced glycation end-products (AGEs) are known to be toxic and pro-inflammatory in mammals. Recently, their presence was unambiguously demonstrated in vivo in stressed Arabidopsis thaliana plants. Currently, information on protein targets, modification sites therein, mediators and mechanisms of plant glycation are being intensively studied. In this chapter, we comprehensively review the methodological approaches for plant glycation research and discuss potential mechanisms of AGE formation under stress conditions. On the basis of these patterns and additional in vitro experiments, the pathways and mechanisms of plant glycation can be proposed.",signatures:"Tatiana Bilova, Uta Greifenhagen, Gagan Paudel, Elena Lukasheva,\nDominic Brauch, Natalia Osmolovskaya, Elena Tarakhovskaya, Gerd\nUlrich Balcke, Alain Tissier, Thomas Vogt, Carsten Milkowski,\nClaudia Birkemeyer, Ludger Wessjohann and Andrej Frolov",downloadPdfUrl:"/chapter/pdf-download/49634",previewPdfUrl:"/chapter/pdf-preview/49634",authors:[{id:"90174",title:"Prof.",name:"Alain",surname:"Tissier",slug:"alain-tissier",fullName:"Alain Tissier"},{id:"177604",title:"Dr.",name:"Tatiana",surname:"Bilova",slug:"tatiana-bilova",fullName:"Tatiana Bilova"},{id:"177605",title:"MSc.",name:"Uta",surname:"Greifenhagen",slug:"uta-greifenhagen",fullName:"Uta Greifenhagen"},{id:"177606",title:"BSc.",name:"Gagan",surname:"Paudel",slug:"gagan-paudel",fullName:"Gagan Paudel"},{id:"177607",title:"BSc.",name:"Elena",surname:"Lukasheva",slug:"elena-lukasheva",fullName:"Elena Lukasheva"},{id:"177608",title:"MSc.",name:"Dominic",surname:"Brauch",slug:"dominic-brauch",fullName:"Dominic Brauch"},{id:"177609",title:"Dr.",name:"Natalia",surname:"Osmolovskaya",slug:"natalia-osmolovskaya",fullName:"Natalia Osmolovskaya"},{id:"177610",title:"Dr.",name:"Elena",surname:"Tarakhovskaya",slug:"elena-tarakhovskaya",fullName:"Elena Tarakhovskaya"},{id:"177611",title:"Dr.",name:"Gerd Ulrich",surname:"Balcke",slug:"gerd-ulrich-balcke",fullName:"Gerd Ulrich Balcke"},{id:"177612",title:"Dr.",name:"Thomas",surname:"Vogt",slug:"thomas-vogt",fullName:"Thomas Vogt"},{id:"177613",title:"Dr.",name:"Carsten",surname:"Milkowski",slug:"carsten-milkowski",fullName:"Carsten Milkowski"},{id:"177614",title:"Dr.",name:"Claudia",surname:"Birkemeyer",slug:"claudia-birkemeyer",fullName:"Claudia Birkemeyer"},{id:"177615",title:"Prof.",name:"Ludger",surname:"Aloisius Wessjohann",slug:"ludger-aloisius-wessjohann",fullName:"Ludger Aloisius Wessjohann"},{id:"177619",title:"Dr.",name:"Andrej",surname:"Frolov",slug:"andrej-frolov",fullName:"Andrej Frolov"}],corrections:null},{id:"49699",title:"Stress-Induced Proteins in Recalcitrant Seeds During Deep Dormancy and Early Germination",doi:"10.5772/61958",slug:"stress-induced-proteins-in-recalcitrant-seeds-during-deep-dormancy-and-early-germination",totalDownloads:1877,totalCrossrefCites:2,totalDimensionsCites:2,hasAltmetrics:0,abstract:"The role and functions of dehydrins and heat shock proteins in seeds (especially in desiccation-sensitive recalcitrant seeds) are discussed.",signatures:"Marina I. Azarkovich",downloadPdfUrl:"/chapter/pdf-download/49699",previewPdfUrl:"/chapter/pdf-preview/49699",authors:[{id:"157230",title:"Dr.",name:"Marina",surname:"Azarkovich",slug:"marina-azarkovich",fullName:"Marina Azarkovich"}],corrections:null},{id:"49737",title:"Molecules and Methods for the Control of Biotic Stress Especially the Insect Pests — Present Scenario and Future Perspective",doi:"10.5772/62034",slug:"molecules-and-methods-for-the-control-of-biotic-stress-especially-the-insect-pests-present-scenario-",totalDownloads:1625,totalCrossrefCites:0,totalDimensionsCites:1,hasAltmetrics:0,abstract:"Continuous increase in population has unbalanced the demand and supply of agricultural produce. In this scenario, food security in a sustainable manner is being challenged due to several factors. Insect pests are considered as one of the major factors, which accounts for 35–100% crop damage, worldwide. Synthetic insecticides contributed significantly, but several safety concerns are associated with them. Transgenic crops with enhanced biotic or abiotic stress tolerance have shown promising contribution in achieving greater crop productivity. Transgenic cotton expressing Cry toxin of Bacillus thuringiensis has tremendously increased the production as well as the societal status of farmers in our country. However, a concomitant increase in the population of minor pests like aphids, whiteflies and others has demanded certain new approaches. Researchers have isolated several other toxic proteins like lectins, protease inhibitors, amylase inhibitors, chitinases, and tried various novel approaches like gene pyramiding, tissue specific expression and modulation in metabolites expression to combat emerging problems of insect pests. Conversely, the emergence of a new type of crop insect pests demands more specific effort for each insect. Besides this, there are several safety and ethical concerns that are associated with the use of genetically modified crops, which also need to be resolved as per demand. Development of a dedicated scientific forum for the proper demonstration of advantages and disadvantages of genetically modified crops to the citizens at ground level might be useful in resolving the societal and ethical concerns in our country.",signatures:"Santosh Kumar Upadhyay and Sudhir P. Singh",downloadPdfUrl:"/chapter/pdf-download/49737",previewPdfUrl:"/chapter/pdf-preview/49737",authors:[{id:"176090",title:"Dr.",name:"Sudhir",surname:"Singh",slug:"sudhir-singh",fullName:"Sudhir Singh"},{id:"176153",title:"Dr.",name:"Santosh",surname:"Upadhyay",slug:"santosh-upadhyay",fullName:"Santosh Upadhyay"}],corrections:null},{id:"49586",title:"Evaluation of TMV Lesion Formation and Timing of Signal Transduction during Induction of Systemic Acquired Resistance (SAR) in Tobacco with a Computer-Assisted Method",doi:"10.5772/61828",slug:"evaluation-of-tmv-lesion-formation-and-timing-of-signal-transduction-during-induction-of-systemic-ac",totalDownloads:1858,totalCrossrefCites:1,totalDimensionsCites:3,hasAltmetrics:0,abstract:"Nicotiana tabacum L. cv. Xanthi nc plants were inoculated with tobacco mosaic virus (TMV) in order to develop a method for evaluation of lesion size and its distribution characteristics during the induction of systemic acquired resistance (SAR). All necrotic lesions were scored with an image analysis software and subjected to statistical analysis. The diminished lesion size and its right-skewed, non-normal distribution seem to be an important feature of SAR response. The results showed that the degree of induced resistance differs according to the position of the leaf on the plant’s shoot. In order to detect the timing of signal transduction from TMV infected leaves to distant ones, the infected leaves were removed from the tobacco plants at different time intervals. When the infected leaves were removed after 4 days, the SAR was always induced on the distant leaves indicating complete signal transduction within 4 days.",signatures:"Zoltán Á. Nagy, György Kátay, Gábor Gullner and Attila L. Ádám",downloadPdfUrl:"/chapter/pdf-download/49586",previewPdfUrl:"/chapter/pdf-preview/49586",authors:[{id:"176268",title:"Dr.",name:"Attila",surname:"Adam",slug:"attila-adam",fullName:"Attila Adam"},{id:"176300",title:"Dr.",name:"Zoltán",surname:"Nagy",slug:"zoltan-nagy",fullName:"Zoltán Nagy"},{id:"176301",title:"Dr.",name:"György",surname:"Kátay",slug:"gyorgy-katay",fullName:"György Kátay"},{id:"176308",title:"Dr.",name:"Gábor",surname:"Gullner",slug:"gabor-gullner",fullName:"Gábor Gullner"}],corrections:null},{id:"49206",title:"Abiotic Stress Alleviation with Brassinosteroids in Plant Roots",doi:"10.5772/61336",slug:"abiotic-stress-alleviation-with-brassinosteroids-in-plant-roots",totalDownloads:2458,totalCrossrefCites:2,totalDimensionsCites:7,hasAltmetrics:0,abstract:"This chapter covers the advances in establishment and optimization of brassinosteroids (BRs) in the alleviation of abiotic stresses such as water, salinity, temperature, and heavy metals in plant system, especially roots. Plant roots regulate their developmental and physiological processes in response to various internal and external stimuli. Studies are in progress to improve plant root adaptations to stress factors. BRs are a group of steroidal hormones that play important roles in a wide range of developmental phenomena, and recently they became an alleviation agent for stress tolerance in plants. This review is expected to provide a resource for researchers interested in abiotic stress alleviation with BRs.",signatures:"Sevgi Marakli and Nermin Gozukirmizi",downloadPdfUrl:"/chapter/pdf-download/49206",previewPdfUrl:"/chapter/pdf-preview/49206",authors:[{id:"176077",title:"Dr.",name:"Sevgi",surname:"Marakli",slug:"sevgi-marakli",fullName:"Sevgi Marakli"},{id:"185345",title:"Dr.",name:"Nermin",surname:"Gozukirmizi",slug:"nermin-gozukirmizi",fullName:"Nermin Gozukirmizi"}],corrections:null},{id:"49796",title:"Role of Phytochelatins in Redox Caused Stress in Plants and Animals",doi:"10.5772/62160",slug:"role-of-phytochelatins-in-redox-caused-stress-in-plants-and-animals",totalDownloads:2217,totalCrossrefCites:1,totalDimensionsCites:8,hasAltmetrics:1,abstract:"Varied environmental compartments (such as soil and water) potentially contaminated with different metals/metalloids can impact the health of both plants and animals/humans. Trace amounts of Cu, Mn, Mo, Ni and Zn are beneficial for higher plants, whereas, Cr, Cu, Co, Mn, Mo, Se, V and Zn are known as the micronutrient metal/metalloids for animals/humans. However, elevated levels of the metals/metalloids can cause severe toxic consequences in both plants and animals/humans. Common in plants and animals/humans, phytochelatins (PCs), the principal non-protein, S-rich, thiolate peptides, protect (through different mechanisms) cellular functions and metal/metalloid homeostasis by performing their chelation and/or detoxification. With the major aim of broadening the current knowledge on the subject, this chapter (a) overviews PCs’ role and modulation separately in metal/metalloid-exposed plants and animals/humans; (b) discusses major methods for determination of PCs and bioassays for enzymes involved in PC synthesis; (c) evaluates the connection of PCs with bionanoparticles; and finally (d) highlights so far unexplored aspects in the present context.",signatures:"Miguel Angel Merlos Rodrigo, Naser A. Anjum, Zbynek Heger,\nOndrej Zitka, Adam Vojtech, Eduarda Pereira and Rene Kizek",downloadPdfUrl:"/chapter/pdf-download/49796",previewPdfUrl:"/chapter/pdf-preview/49796",authors:[{id:"16205",title:"Dr.",name:"Rene",surname:"Kizek",slug:"rene-kizek",fullName:"Rene Kizek"},{id:"142677",title:"Dr.",name:"Vojtech",surname:"Adam",slug:"vojtech-adam",fullName:"Vojtech Adam"},{id:"155784",title:"Prof.",name:"Eduarda",surname:"Pereira",slug:"eduarda-pereira",fullName:"Eduarda Pereira"},{id:"177390",title:"Dr.",name:"Marketa",surname:"Vaculovicova",slug:"marketa-vaculovicova",fullName:"Marketa Vaculovicova"},{id:"177407",title:"MSc.",name:"Miguel",surname:"Merlos Rodrigo",slug:"miguel-merlos-rodrigo",fullName:"Miguel Merlos Rodrigo"},{id:"177408",title:"Prof.",name:"Naser",surname:"Anjum",slug:"naser-anjum",fullName:"Naser Anjum"},{id:"177409",title:"MSc.",name:"Zbynek",surname:"Heger",slug:"zbynek-heger",fullName:"Zbynek Heger"},{id:"177410",title:"Dr.",name:"Ondrej",surname:"Zitka",slug:"ondrej-zitka",fullName:"Ondrej Zitka"}],corrections:null},{id:"49686",title:"Signalling Crosstalk of Plant Defence Responses to Xylem-invading Pathogens",doi:"10.5772/61955",slug:"signalling-crosstalk-of-plant-defence-responses-to-xyleminvading-pathogens",totalDownloads:1863,totalCrossrefCites:1,totalDimensionsCites:2,hasAltmetrics:0,abstract:"Xylem is a plant vascular tissue that transports water and dissolved minerals from the roots to the rest of the plant. It consists of specialized water-conducting tracheary elements, supporting fibre cells and storage parenchyma cells. Certain plant pathogenic fungi, oomycetes and bacteria have evolved strategies to invade xylem vessels and cause highly destructive vascular wilt diseases that affect the crop production and forest ecosystems worldwide. In this chapter, we consider the molecular mechanisms of root-specific defence responses against vascular wilt pathogens, with an emphasis on the most important and well-studied fungal (Verticillium spp. and Fusarium oxysporum) and bacterial (Xanthomonas spp. and Ralstonia solanacearum) pathogens. In particular, we present the current understanding of plant immune responses, from invasion perception to signal transduction and termination. Furthermore, we address the role of specific transcription factors involved in plant immunity and their regulatory network. We also highlight the crucial roles of phytohormones as signalling molecules in local and systemic defence responses. Finally, we summarize the current knowledge of plant defence responses to xylem-invading pathogens to devise new strategies and methods for controlling these destructive plant pathogens.",signatures:"Sabina Berne and Branka Javornik",downloadPdfUrl:"/chapter/pdf-download/49686",previewPdfUrl:"/chapter/pdf-preview/49686",authors:[{id:"154940",title:"Prof.",name:"Branka",surname:"Javornik",slug:"branka-javornik",fullName:"Branka Javornik"},{id:"177633",title:"Dr.",name:"Sabina",surname:"Berne",slug:"sabina-berne",fullName:"Sabina Berne"}],corrections:null},{id:"49162",title:"Abscisic Acid Signalling as a Target for Enhancing Drought Tolerance",doi:"10.5772/61317",slug:"abscisic-acid-signalling-as-a-target-for-enhancing-drought-tolerance",totalDownloads:1965,totalCrossrefCites:2,totalDimensionsCites:3,hasAltmetrics:0,abstract:"Abscisic acid (ABA) is a vital hormone that confers abiotic stress tolerance in plants. The identification of PYR/PYL/RCAR proteins as bona fide ABA receptors and the subsequent elucidation of the structural mechanisms of the core ABA signalling pathway in recent years has provided new and powerful insights in targeting ABA signalling to enhance abiotic stress tolerance in agriculture. This chapter reviews the components and molecular mechanisms of the core ABA signalling pathway, as revealed by X-ray crystallography studies, and how these knowledge led to preliminary efforts in novel biotechnological developments to improve stress tolerance in plants.",signatures:"Ley Moy Ng",downloadPdfUrl:"/chapter/pdf-download/49162",previewPdfUrl:"/chapter/pdf-preview/49162",authors:[{id:"175961",title:"Dr.",name:"Ley Moy",surname:"Ng",slug:"ley-moy-ng",fullName:"Ley Moy Ng"}],corrections:null},{id:"49274",title:"Reactive Oxygen Species and Antioxidant Enzymes Involved in Plant Tolerance to Stress",doi:"10.5772/61368",slug:"reactive-oxygen-species-and-antioxidant-enzymes-involved-in-plant-tolerance-to-stress",totalDownloads:4924,totalCrossrefCites:47,totalDimensionsCites:107,hasAltmetrics:0,abstract:"Plants are continuously exposed to several stress factors in field, which affect their production. These environmental adversities generally induce the accumulation of reactive oxygen species (ROS), which can cause severe oxidative damage to plants. ROS are toxic molecules found in various subcellular compartments. The equilibrium between the production and detoxification of ROS is sustained by enzymatic and nonenzymatic antioxidants. Due to advances in molecular approaches during the last decades, nowadays it is possible to develop economically important transgenic crops that have increased tolerance to stresses. This chapter discusses the oxidative stress and damage to plants. In addition, it reports the involvement of antioxidant enzymes in the tolerance of plants to various stresses.",signatures:"Andréia Caverzan, Alice Casassola and Sandra Patussi Brammer",downloadPdfUrl:"/chapter/pdf-download/49274",previewPdfUrl:"/chapter/pdf-preview/49274",authors:[{id:"176303",title:"Dr.",name:"Alice",surname:"Casassola",slug:"alice-casassola",fullName:"Alice Casassola"},{id:"176409",title:"Dr.",name:"Andréia",surname:"Caverzan",slug:"andreia-caverzan",fullName:"Andréia Caverzan"},{id:"176410",title:"Dr.",name:"Sandra",surname:"Patussi Brammer",slug:"sandra-patussi-brammer",fullName:"Sandra Patussi Brammer"}],corrections:null},{id:"49852",title:"Antioxidant Enzyme Activities and Abiotic Stress Tolerance Relationship in Vegetable Crops",doi:"10.5772/62235",slug:"antioxidant-enzyme-activities-and-abiotic-stress-tolerance-relationship-in-vegetable-crops",totalDownloads:3108,totalCrossrefCites:20,totalDimensionsCites:57,hasAltmetrics:0,abstract:"Climatic changes can cause serious reductions in yield and crop quality. Under the threat of climatic changes, one of the precautions to cope is selection and development of resistant vegetable genotypes to abiotic stresses. Several physiological and biochemical reactions and different tolerance levels can occur according to plant species. When plants are subjected to environmental stresses such as salinity, drought, temperature extremes, herbicide treatment and mineral deficiency, the balance between the production of reactive oxygen species (ROS) and the quenching activity of antioxidants is upset, often resulting in oxidative damage. Since activated oxygen species can disrupt normal metabolism through oxidative damage to lipids, protein and nucleic acids, plants possess a number of antioxidant enzymes that protect them from these cytotoxic effects. To control the level of ROS and to protect cells under stress conditions, plant tissues contain several enzymes for scavenging ROS. The high levels of antioxidative enzyme activities were determined in the tolerant genotypes of tomatoes, eggplant, peppers, cucumbers, melons, squash, beans, okra, etc. to several abiotic stress factors. Both the whole plant and in vitro callus culture experiments gave similar results. Antioxidant enzymes can be useful for screening to determine the tolerant and sensitive plant genotypes against abiotic stresses.",signatures:"Sebnem Kusvuran, Sevinç Kiran and S. Sebnem Ellialtioglu",downloadPdfUrl:"/chapter/pdf-download/49852",previewPdfUrl:"/chapter/pdf-preview/49852",authors:[{id:"139032",title:"Associate Prof.",name:"Sebnem",surname:"Kusvuran",slug:"sebnem-kusvuran",fullName:"Sebnem Kusvuran"},{id:"142251",title:"Prof.",name:"Sebnem",surname:"Ellialtioglu",slug:"sebnem-ellialtioglu",fullName:"Sebnem Ellialtioglu"},{id:"176220",title:"Dr.",name:"Sevinc",surname:"Uslu Kıran",slug:"sevinc-uslu-kiran",fullName:"Sevinc Uslu Kıran"}],corrections:null},{id:"49690",title:"Role of ABA in Arabidopsis Salt, Drought, and Desiccation Tolerance",doi:"10.5772/61957",slug:"role-of-aba-in-arabidopsis-salt-drought-and-desiccation-tolerance",totalDownloads:3457,totalCrossrefCites:26,totalDimensionsCites:52,hasAltmetrics:0,abstract:"The ability of plants to respond to environmental stimuli is essential to plant survival. Abscisic acid (ABA) is a phytohormone with roles at various stages of plant development. ABA also plays a major role in mediating physiological responses to environmental stresses such as salt, osmotic, and cold stress. Plant responses to environmental stress have been widely studied in the model plant Arabidopsis thaliana and ABA signaling mechanisms elucidated. In general, the adaptive responses of plants to various stress conditions can be either ABA-dependent or ABA-independent. Here we focus on the role of ABA in stress signaling and abiotic stress tolerance. We describe the intrinsic mechanisms that confer stress tolerance via ABA, as well as how ABA-regulated gene products play a role in salt and drought tolerance at different stages of the life cycle. In addition, the contribution of ABA to regulation of stomatal aperture and therefore desiccation tolerance will be discussed. Understanding ABA signaling mechanisms in abiotic stress provides avenues for improving plant performance.",signatures:"V. C. Dilukshi Fernando and Dana F. Schroeder",downloadPdfUrl:"/chapter/pdf-download/49690",previewPdfUrl:"/chapter/pdf-preview/49690",authors:[{id:"46453",title:"Dr.",name:"Dana",surname:"Schroeder",slug:"dana-schroeder",fullName:"Dana Schroeder"},{id:"176279",title:"Dr.",name:"V.C. Dilukshi",surname:"Fernando",slug:"v.c.-dilukshi-fernando",fullName:"V.C. Dilukshi Fernando"}],corrections:null},{id:"49717",title:"Rootstock Breeding for Abiotic Stress Tolerance in Citrus",doi:"10.5772/62047",slug:"rootstock-breeding-for-abiotic-stress-tolerance-in-citrus",totalDownloads:4106,totalCrossrefCites:15,totalDimensionsCites:26,hasAltmetrics:0,abstract:"Citrus species are the most widely produced fruit crops in the world. Citrus fruits are mainly produced in coastal areas in several countries as well as Mediterranean region, and production in these regions is affected by both biotic and abiotic stresses, including drought, extreme temperature, salinity, citrus canker, citrus tristeza virus, citrus greening, and others. The use of rootstocks in fruit production includes not only stronger resistance against pathogens but also a higher tolerance to abiotic stress conditions such as salinity, heavy metals, nutrient stress, water stress, and alkalinity. There is extensive genetic diversity in citrus which provides several materials to be used as rootstocks against abiotic stress. In this work, we tried to provide an overview of the abiotic stresses in citrus by combining literature with our studies, role of citrus rootstocks commercially used against abiotic stresses and rootstock breeding in citrus.",signatures:"Berken Cimen and Turgut Yesiloglu",downloadPdfUrl:"/chapter/pdf-download/49717",previewPdfUrl:"/chapter/pdf-preview/49717",authors:[{id:"103938",title:"Prof.",name:"Turgut",surname:"Yesiloglu",slug:"turgut-yesiloglu",fullName:"Turgut Yesiloglu"},{id:"175905",title:"MSc.",name:"Berken",surname:"Cimen",slug:"berken-cimen",fullName:"Berken Cimen"}],corrections:null},{id:"49752",title:"Cowpea Breeding for Drought Tolerance — From Brazil to World",doi:"10.5772/62000",slug:"cowpea-breeding-for-drought-tolerance-from-brazil-to-world",totalDownloads:2346,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"The aims of this chapter are to (i) present the importance of the cowpea crop, (ii) demonstrate problems related to drought, (iii) describe aspects related to flower structure and hybridization, and (iv) reveal how the genotype selection with tolerance to water deficit will promote increase of the yield in this culture. This chapter describes that Nigeria, Niger, and Brazil are the leading producers of cowpea crop worldwide, and this crop has a large influence on the economy of these countries. However, the drought problem can be frequently observed in areas with agricultural potential, with negative effects on production components and a consequent decrease of the yield in this culture. Breeding programs aimed at drought tolerance using selection strategies linked to genetic, biochemical, physiological, nutritional, and agronomic characteristics can help increase the yield and reduce losses promoted by the drought. In addition, flower structure and hybridization technique used in Núcleo de Pesquisa Vegetal Básica e Aplicada (NPVBA/UFRA) are presented, as well as populations are evaluated and plant management are explained in detail. This chapter describes the results obtained in other breeding programs aimed at drought tolerance and also explains the potential uses to increase the crop yield.",signatures:"Maria Antonia Machado Barbosa, Allan Klynger da Silva Lobato,\nMilton Hélio Lima da Silva, Gabriel Mascarenhas Maciel and\nDouglas José Marques",downloadPdfUrl:"/chapter/pdf-download/49752",previewPdfUrl:"/chapter/pdf-preview/49752",authors:[{id:"79200",title:"Prof.",name:"Allan",surname:"Klynger da Silva Lobato",slug:"allan-klynger-da-silva-lobato",fullName:"Allan Klynger da Silva Lobato"},{id:"176258",title:"MSc.",name:"Maria",surname:"Barbosa",slug:"maria-barbosa",fullName:"Maria Barbosa"},{id:"176259",title:"Dr.",name:"Milton",surname:"Silva",slug:"milton-silva",fullName:"Milton Silva"}],corrections:null},{id:"49800",title:"Sunflower Breeding for Resistance to Abiotic and Biotic Stresses",doi:"10.5772/62159",slug:"sunflower-breeding-for-resistance-to-abiotic-and-biotic-stresses",totalDownloads:3606,totalCrossrefCites:7,totalDimensionsCites:18,hasAltmetrics:0,abstract:"Due to a specific structure of its main organs (root, stem, leaves, and head), sunflower can be successfully grown on marginal soils and in semiarid conditions, and it is more resistant to abiotic stresses, than other field crops. Unfortunately, it is very sensitive to biotic stresses.",signatures:"Dragan Škorić",downloadPdfUrl:"/chapter/pdf-download/49800",previewPdfUrl:"/chapter/pdf-preview/49800",authors:[{id:"176099",title:"Dr.",name:"Dragan",surname:"Skoric",slug:"dragan-skoric",fullName:"Dragan Skoric"}],corrections:null},{id:"49669",title:"Drought Adaptation in Millets",doi:"10.5772/61929",slug:"drought-adaptation-in-millets",totalDownloads:3573,totalCrossrefCites:15,totalDimensionsCites:27,hasAltmetrics:0,abstract:"Millets are major food and feed sources in the developing world especially in the semi-arid tropical regions of Africa and Asia. The most widely cultivated millets are pearl millet [Pennisetum glaucum (L.) R. Br.], finger millet [Eleusine coracana (L.) Gaertn], foxtail millet [Setaria italica (L.) P. Beauvois], Japanese barnyard millet [Echinochloa esculneta (A. Braun) H. Scholz], Indian Barnyard millet [Echinochloa frumetacea Link], kodo millet [Paspalum scrobiculatum L.], little millet [Panicum sumatrense Roth.ex.Roem. & Schult.], proso millet [Panicum miliaceum L.], tef [Eragrostis tef (Zucc.) Trotter] and fonio or acha [Digitaria exilis (Kippist) Stapf and D. iburua Stapf]. Millets are resilient to extreme environmental conditions especially to inadequate moisture and are rich in nutrients. Millets are also considered to be a healthy food, mainly due to the lack of gluten (a substance that causes coeliac disease) in their grain. Despite these agronomic, nutritional and health-related benefits, millets produce very low yield compared to major cereals such as wheat and rice. This extremely low productivity is related to the challenging environment in which they are extensively cultivated and to the little research investment in these crops. Recently, several national and international initiatives have begun to support the improvement of diverse millet types.",signatures:"Zerihun Tadele",downloadPdfUrl:"/chapter/pdf-download/49669",previewPdfUrl:"/chapter/pdf-preview/49669",authors:[{id:"176084",title:"Dr.",name:"Zerihun",surname:"Tadele",slug:"zerihun-tadele",fullName:"Zerihun Tadele"}],corrections:null},{id:"49864",title:"Plant Integrity – The Important Factor of Adaptability to Stress Conditions",doi:"10.5772/62306",slug:"plant-integrity-the-important-factor-of-adaptability-to-stress-conditions",totalDownloads:2162,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:'Crop production, research of crop productivity, tolerance to abiotic and biotic stresses, plant disease, and pests all represent the problem of plant integrity. Plants represent an integrated system of units, which are responsible for its resistance to adverse environmental conditions on the basis of the evaluation of characteristics both aboveground and in the roots. This "complete unit" (root and shoot) has an influence on the formation of seeds, the quality of which may affect subsequent growth, development, and stress tolerance of the filial generation. Properties of the roots predominantly influence (especially at drought stress conditions) growth, development, and the metabolic processes in the aboveground part of the plant. The seed traits affect the filial generation root morphology at the beginning of the vegetation period (especially length, surface, depth of root penetration, and also root weight). In the biology of the seeds, roots, yield formation, stress tolerance, etc., attention needs to be paid to plant integrity and adaptability during variable environmental conditions. Every plant, and its traits, is a result of all the plant’s activities. This is important for plant breeding. For example, it is possible to provide selection for cultivar traits at seed germination. Quality of the embryonic traits is important for subsequent growth and development. In the juvenile phase, and in later stages, the same genotype is still active. This is is among the main reasons for studying plant integrity.',signatures:"Ladislav Bláha and Tomáš Středa",downloadPdfUrl:"/chapter/pdf-download/49864",previewPdfUrl:"/chapter/pdf-preview/49864",authors:[{id:"156680",title:"Dr.",name:"Ladislav",surname:"Bláha",slug:"ladislav-blaha",fullName:"Ladislav Bláha"},{id:"177241",title:"Dr.",name:"Tomás",surname:"Středa",slug:"tomas-streda",fullName:"Tomás Středa"}],corrections:null},{id:"49646",title:"Plant Evolution in Response to Abiotic and Biotic Stressors at “Rear-edge” Range Boundaries",doi:"10.5772/61976",slug:"plant-evolution-in-response-to-abiotic-and-biotic-stressors-at-rear-edge-range-boundaries",totalDownloads:1774,totalCrossrefCites:1,totalDimensionsCites:3,hasAltmetrics:0,abstract:"Most species in the mustard family are restricted to higher elevations and latitudes where they also have restricted local spatial distributions. In this chapter, we describe a novel hypothesis for the development of low-elevation range limits in upland mustard species. The hypothesis suggests that defense regulation of glucosinolates could underlie the evolution of the spatially restricted distributions. A list of testable predictions is presented to evaluate the hypothesis. An interdisciplinary Ecological Genomics approach is needed to test the predictions; therefore, we also describe the field of Ecological Genomics. Although there is already support for some of the predictions, which we discuss, most of the predictions remain untested. Therefore, we also describe several tests that help evaluate each of the predictions.",signatures:"Gunbharpur S. Gill, Riston Haugen, Jesse Larson, Jason Olsen and\nDavid H. Siemens",downloadPdfUrl:"/chapter/pdf-download/49646",previewPdfUrl:"/chapter/pdf-preview/49646",authors:[{id:"176260",title:"M.Sc.",name:"Gunbharpur",surname:"Gill",slug:"gunbharpur-gill",fullName:"Gunbharpur Gill"},{id:"181689",title:"Dr.",name:"David",surname:"Siemens",slug:"david-siemens",fullName:"David Siemens"},{id:"181737",title:"MSc.",name:"Riston",surname:"Haugen",slug:"riston-haugen",fullName:"Riston Haugen"},{id:"181738",title:"BSc.",name:"Jesse",surname:"Larson",slug:"jesse-larson",fullName:"Jesse Larson"},{id:"181739",title:"MSc.",name:"Jason",surname:"Olsen",slug:"jason-olsen",fullName:"Jason Olsen"}],corrections:null},{id:"49604",title:"The Trials and Tribulations of the Plant Male Gametophyte — Understanding Reproductive Stage Stress Tolerance",doi:"10.5772/61671",slug:"the-trials-and-tribulations-of-the-plant-male-gametophyte-understanding-reproductive-stage-stress-to",totalDownloads:2413,totalCrossrefCites:12,totalDimensionsCites:26,hasAltmetrics:0,abstract:"Yield and productivity of many crop species depend on successful reproductive development to produce seeds or fruits for human nutrition. Plants determine the right time to flower based on environmental cues (day length, temperature) and angiosperms have evolved a plethora of mechanisms to adapt flowering to specific environmental conditions. Despite these adaptation mechanisms, fertilisation and seed production remain subject to the reigning weather conditions before and during flowering. To fertilise the immobile female gametes inside the ovule, the male gametophytes need to be dispersed in a hostile environment. In crop plants, unexpected inclement weather conditions during male gametophyte development and pollen dispersal are often associated with dramatic yield losses. Molecular and physiological studies are gradually making progress in identifying genes and processes that control various aspects of pollen development, but the many intricacies involved in environmental control of pollen development and – in particular – regulation of male fertility remain poorly understood. The aim of this paper is to draw attention to the enormous amount of complexity and biodiversity that exist in angiosperm male gametophyte development. A better understanding of the strategies that exist in adapting pollen production and fertility to environmental challenges may ultimately benefit improvement of abiotic stress tolerance in major food crops.",signatures:"Ettore Pacini and Rudy Dolferus",downloadPdfUrl:"/chapter/pdf-download/49604",previewPdfUrl:"/chapter/pdf-preview/49604",authors:[{id:"176305",title:"Dr.",name:"Rudy",surname:"Dolferus",slug:"rudy-dolferus",fullName:"Rudy Dolferus"}],corrections:null}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"},subseries:null,tags:null},relatedBooks:[{type:"book",id:"371",title:"Abiotic Stress in Plants",subtitle:"Mechanisms and Adaptations",isOpenForSubmission:!1,hash:"588466f487e307619849d72389178a74",slug:"abiotic-stress-in-plants-mechanisms-and-adaptations",bookSignature:"Arun Shanker and B. 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This book contains chapters on recent developments in studies of landscape architecture. For this reason I believe the book would be useful to the relevant professional disciplines.",isbn:null,printIsbn:"978-953-51-0654-8",pdfIsbn:"978-953-51-5304-7",doi:"10.5772/2761",price:139,priceEur:155,priceUsd:179,slug:"landscape-planning",numberOfPages:374,isOpenForSubmission:!1,isSalesforceBook:!1,isNomenclature:!1,hash:"3c7b088d1bfbcf17d7f2fe6f47137af2",bookSignature:"Murat Ozyavuz",publishedDate:"June 13th 2012",coverURL:"https://cdn.intechopen.com/books/images_new/2406.jpg",keywords:null,numberOfDownloads:67009,numberOfWosCitations:50,numberOfCrossrefCitations:19,numberOfDimensionsCitations:67,numberOfTotalCitations:136,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"August 25th 2011",dateEndSecondStepPublish:"November 18th 2011",dateEndThirdStepPublish:"January 27th 2012",dateEndFourthStepPublish:"February 26th 2012",dateEndFifthStepPublish:"April 26th 2012",dateConfirmationOfParticipation:null,remainingDaysToSecondStep:"11 years",secondStepPassed:!0,areRegistrationsClosed:!0,currentStepOfPublishingProcess:5,editedByType:"Edited by",kuFlag:!1,biosketch:null,coeditorOneBiosketch:null,coeditorTwoBiosketch:null,coeditorThreeBiosketch:null,coeditorFourBiosketch:null,coeditorFiveBiosketch:null,editors:[{id:"93073",title:"Dr.",name:"Murat",middleName:null,surname:"Ozyavuz",slug:"murat-ozyavuz",fullName:"Murat Ozyavuz",profilePictureURL:"https://mts.intechopen.com/storage/users/93073/images/549_n.jpg",biography:"Assoc. Prof. Dr. Murat Ozyavuz was born in 1976 in Turkey and lived there until coming to Ankara (Turkey) in 1999. He studied Landscape Architecture at the Ankara University and obtained B.S. degree in 1999. He obtained his M.S. and Ph.D. degrees from the Landscape Architecture Department of Institute of Natural and Applied Sciences in 2003 and July 2008, respectively (M. S. Thesis, Arboretum Planning Principles and Thrace University Güllapoğlu Arboretum Landscape Planning Studies, Ph.D. thesis, Planning of İğneada – Demirköy Part of Yildiz Mountains as a Biosphere Reserve). During his Ph.D. studies, he mostly worked on Landscape Planning, Protected Areas, Geographic Information Systems and Remote Sensing. Now, Dr. Ozyavuz is an Associate Professor at the Department of Landscape Architecture, Faculty of Fine Arts, Design and Architect, Namık Kemal University. He has many national and international publications and has worked on many research projects.",institutionString:null,position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"5",totalChapterViews:"0",totalEditedBooks:"2",institution:{name:"Namık Kemal University",institutionURL:null,country:{name:"Turkey"}}}],coeditorOne:null,coeditorTwo:null,coeditorThree:null,coeditorFour:null,coeditorFive:null,topics:[{id:"849",title:"Landscape Ecology",slug:"landscape-ecology"}],chapters:[{id:"37552",title:"Protected Areas",slug:"protected-areas",totalDownloads:2227,totalCrossrefCites:0,authors:[{id:"93073",title:"Dr.",name:"Murat",surname:"Ozyavuz",slug:"murat-ozyavuz",fullName:"Murat Ozyavuz"}]},{id:"37553",title:"Land Use/Cover Classification Techniques Using Optical Remotely Sensed Data in Landscape 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Venkateswarlu",coverURL:"https://cdn.intechopen.com/books/images_new/371.jpg",editedByType:"Edited by",editors:[{id:"58592",title:"Dr.",name:"Arun",surname:"Shanker",slug:"arun-shanker",fullName:"Arun Shanker"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}}]},chapter:{item:{type:"chapter",id:"58971",title:"Lactobacillus Species in Breast Milk",doi:"10.5772/intechopen.72639",slug:"lactobacillus-species-in-breast-milk",body:'Microbiota is a substantial collection of genetic and bioactive materials responsible for building and regulating our defense systems. Bacteria and their intestinal microbial proportions modulate the immune system, greatly affecting the health and illness of an individual. Gastrointestinal flora is in close and continuous contact with epithelial and immune cells. This constant stimulation is essential for the development and functioning of the immune system [1]. These types of bacteria that colonize the guts of a newborn determine how the system develops, acting as an important antigenic stimulus for developing the immune response.
In the last 20 years, probiotics, bifidobacteria,
Respiratory and gastrointestinal tract infections are a significant problem for young children attending daycare centers or preschool, especially in the winter season. Common infectious diseases are facilitated by a general immaturity of the immune system and of the respiratory and gastrointestinal tract function [4]. An increased number of acute diseases translate into a significant financial burden for both the family and society. The increased costs are related to medical care visits and medication as well as to time away from work and/or for payment for someone to look after a sick child [5].
The most widely used probiotic species, which belong to the genera
Control group | Experimental group | Incidence rate decrease (%) | |
---|---|---|---|
Total infections | 189 | 142 | 30 |
Gastrointestinal infections | 33 | 19 | 46 |
Respiratory infections | 134 | 106 | 26 |
| 121 | 94 | 27 |
| 13 | 12 | 13 |
This strain is also able to colonize the mammary glands when administered to nursing mothers in capsule form. A similar effect on the health of children has been described in other probiotic strains. A multicenter, randomized, double-blind, placebo-controlled trial [4] on 126 healthy children aged 12–48 months with
Disease | Control group | Experimental group | |
---|---|---|---|
Acute gastroenteritis, (number of episodes) | 24 (40.0) (28) | 12 (18.2) (19) | 0.007 |
Rhinitis, (number of episodes) | 24 (40.0) (50) | 22 (33.3) (44) | 0.438 |
Otitis media, (number of episodes) | 13 (21.7) (17) | 8 (12.1) (11) | 0.151 |
Pharyngitis, (number of episodes) | 25 (41.7) (30) | 13 (19.7) (22) | 0.007 |
Laryngitis, (number of episodes) | 14 (23.3) (14) | 6 (9.1) (7) | 0.029 |
Tracheitis, (number of episodes) | 19 (31.7) (30) | 11 (16.7) (16) | 0.048 |
Common infectious diseases observed during the study period [4].
Another bacterium isolated from breast milk that has a positive effect on diseases in infants and children is
Mastitis is a common disease during lactation, affecting 3–33% of lactating mothers. Inflammation of the mammary glands usually has an infectious origin involving staphylococci, streptococci, and/or Corynebacterium. Traditionally,
Bacterial counts from breast milk at the beginning (day 0) and at the end (day 21) of the trial [
Breast pain score at the beginning (day 0) and at the end (day 21) of the trial. Pain is expressed as extremely painful (0) to no pain (10) [
Obesity is viewed as one of the more important public health problems of our time, and the velocity of propagation is highest in children. This can lead to a vicious circle: obese children often become obese adults, and maternal obesity overnourishes the fetus, thereby programming adult size and health with a heightened risk of obesity later in life. Recent scientific advances point to systemic low-grade inflammation and local gut microbiota as contributing factors for overnutrition. The gut microbiota enables the hydrolysis of indigestible polysaccharides into easily absorbable monosaccharides and the activation of lipoprotein lipase by direct action on the villous epithelium. Consequently, glucose is rapidly absorbed and fatty acids are excessively stored, with both processes boosting weight gain.
Bacterial milk composition in obese mothers differs from the bacterial milk composition of mothers with standard body weight [11]. Since breast milk is one of the most important means of colonizing infants with bacteria, there is an idea that there is a relationship between obesity and the transmission of microbial flora from mother to infant. It is known that obese infants and obese children generally have very different microbial flora from infants who are lean and healthy (Figure 3). The results reported by Kalliomäki et al. suggested that gut microbiota deviations predispose individuals toward energy storage and obesity. The genus
Bacterial counts in fecal samples analyzed by fluorescent
Perhaps it would be advisable to think about intervention in cases of obese mothers. When is the right time for such an intervention? We know that some bacteria are transmitted from mother to infant. For an obese mother, it would be most helpful to choose an appropriate intervention before or during pregnancy, in any case before giving birth. If the microbial flora has already been transferred to the infant, it could be optimized during breastfeeding through specific probiotics.
The mucosal microbiota is formed by millions of bacteria. The
Wide utilization of microwave thermotherapy can be observed in the countries of the European Union, the United States, Russia, China, Japan, and many others, including the Czech Republic. Interactions of the electromagnetic (EM) field with the human body have been utilized in medicine (e.g., cardiology, oncology, physiotherapy, and urology) since the late seventieth of the twentieth century. A very important role in this process plays scientific societies, e.g., the European Society for Hyperthermia Oncology (ESHO), which cooperates with STM (Society for Thermal Medicine), and ASHO (Asian Society of Hyperthermia Oncology).
Currently, EM fields are frequently used in a few well-established medical procedures already. Good examples in the area of medical diagnostics are, e.g., computer tomography (CT) and magnetic resonance imaging (MRI). In the area of therapy, we can mention, e.g., electrosurgery and radiofrequency (RF) heating in physiotherapy. Then microwave (MW) hyperthermia and RF + MW ablation in clinical therapy are being used for the treatment of cancer and other diseases.
According to the purpose of how microwaves are used, we can divide the medical applications of microwaves into the following three main groups [1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17]:
Therapy (mostly so-called thermal effects are being used; so-called nonthermal effects are still under discussion).
Diagnostics (based on permittivity measurements, very prospective can be, e.g., an MW differential tomography, UWB radar technology, and MW radiometers).
MW is only a part of the medical system (e.g., microwave technology as the basis of the linear accelerator).
As told above, treatment applications of MW are represented mainly by those based on thermal effects. Thus, we can speak about the MW thermotherapy, which can be generally divided into several basic modalities with respect to the goal temperature level or interval:
Diathermia: which means mild heating up to 41°C at maximum (clinically can be used, e.g., in physiotherapy).
Hyperthermia: this means increasing the temperature in the tumor area up to the interval of 41–45°C (clinically mostly used in oncology).
Thermoablation: this means to increase the temperature over 45°C (clinically can be used, e.g., in urology for BPH treatment and in cardiology for treatments of fibrillation and/or arrhythmia).
For the abovementioned methods of thermotherapy treatments, frequency interval from 1 up to 5600 MHz is mostly used.
As for diagnostics based on the EM field—significant importance for the near future can be identified for the following methods mainly:
MRI and CT (both these diagnostics methods have been largely used since the 80th of the last century already; they both represent the highest possible level of medical diagnostics).
Microwave differential tomography (very prospective as a diagnostics method for the near future, to be used mainly as diagnostics of cancer or for noninvasive temperature measurements).
Microwave UWB diagnostic radar (can be used for the same purposes as microwave differential tomography, but as well for other purposes, such as monitoring of breathing).
Microwave radiometry (can be used for the same purposes as microwave differential tomography, but it works on different physical principles).
As for the frequency spectrum of the EM field (Figure 1), then it is possible to see that MRI is working in the frequency band from 64 to 299 MHz (i.e., the upper part of the RF band); instead, CT then is working in hard X-ray band. The MW frequency band is frequencies from 300 MHz to 300 GHz. The lower part of this frequency band, from 300 MHz to 6 GHz, is very prospective for MW medical imaging. Frequency band above approx. 100 GHz is very prospective for imaging with Terahertz waves. In Figure 1, there is a picture of the frequency spectrum of the EM field.
Frequency spectrum of EM fields.
In this chapter, we will not describe the MRI and CT technology, as it is a well-known and broadly used application of the EM field in medical diagnostics. We will describe and discuss here other methods based on microwave technology mentioned above. The idea of MWs for medical diagnostics is a relatively new area but rapidly developing. The main advantages of MW technology with respect to CT and/or MRI are as follows:
MWs belong to EM nonionizing radiation (such as MRI); instead, CT works in the ionizing part of the EM frequency spectrum.
The system for MW diagnostics is very small and lightweight in comparison with MRI and CT (its dimension and weight are comparable to a notebook, so it will be possible to have it in an ambulance).
MW diagnostic systems have the potential to be at least one order less expensive than either MRI or CT (since the MW technology is being massively used in mobile telecommunications).
And it is important to underline that for MW diagnostics, low power levels (1–20 mW) are used only.
The first four of the following list of thermotherapeutical applications are just largely used in many countries around the world; the last three instead are at this moment in the phase of very promising projects:
Oncology (cancer treatment)
Biological principle utilizes the fact that certain tumor cells are very sensitive to a temperature higher than 41°C, while normal cells generally survive elevated temperatures up to 45°C. And so heating of the tumor region at temperatures of 41–45°C can selectively destroy tumor cells.
Physiotherapy (treatment of rheumatic and skeletal diseases)
Like in hyperthermia, therapeutic effect is caused by the principle of heating biological tissue, but to lower temperatures—usually only up to 41°C. It is used for treating pain in certain rheumatic and degenerative diseases and the treatment of chronic inflammations resistant to antibiotics, often used in rehabilitation and physical therapy.
Urology (BPH treatment)
Microwave thermocoagulation—heating up to temperatures much higher than 45°C, usually around 70°C. An example can be given the microwave treatment of Benign Prostate Hyperplasia, which can replace a complicated surgery.
Cardiology (arrhythmia and fibrillation treatment, microwave angioplasties)
Cardiac catheter thermal ablation is now the standard of care for various cardiac arrhythmia types (irregular heartbeat rhythm). The method uses a catheter terminated with a microwave antenna, which is introduced into either heart of the patient or into partially or totally blocked blood vessels. Heat gained by microwave energy either safely removes the cells inside the human heart causing arrhythmia and/or fibrillation or removes sclerotic plaques deposited on the walls of blood vessels.
Surgery (microwave scalpel, growing implants)
Ophthalmology (retina corrections)
Neurology (stroke identification, brain stimulation)
For here mentioned thermotherapy treatments, it is important to mention that frequencies from the frequency band started approximately at 1 MHz and going up to 10 GHz are mostly used. This frequency range is given by the optimal depth of penetration of EM waves into biological tissue. Thus, this frequency band can achieve the needed depth of effective treatment.
In Prague, the clinical applications of microwave hyperthermia for cancer treatment started in 1981, in cooperation with the Medical Faculty (the Charles University in Prague), the Radiotherapy Institute in Prague, and the Dept. of EM Field (the Czech Technical University in Prague). Since then, microwave hyperthermia has been clinically applied to more than 1000 cancer patients. Mostly added to radiotherapy (RT), a clinical study has been approved as a significantly positive contribution to RT treatment. Recently, a combination of hyperthermia added to proton therapy has been clinically applied in Prague.
Treatment of malignant tumors comprises several techniques usually. In some cases, tumors can be resected by surgery. Radiotherapy and/or chemotherapy can be applied when surgery is not possible or as part of a multidisciplinary approach. A less widely known treatment modality is hyperthermia. It is a therapeutic application of heat in which tumor temperatures are elevated in the range of 41–45°C. The heating of tumor tissue has a cell killing (cytotoxic) effect. However, the cytotoxic effect is small at temperatures below 45°C. Therefore, hyperthermia is always clinically combined with either radiotherapy or chemotherapy. The application of hyperthermia has been proven to increase the therapeutic effect of both radiotherapy and chemotherapy.
The effect of hyperthermia is strongly dependent on the achieved tumor temperatures and heating time. Preclinical research has shown that the cell-killing effect doubles every centigrade, e.g., 1 hour at 42°C is equivalent to half an hour at 43°C. Hypoxic tumors, i.e., tumors with a low level of oxygen, are more resistant to ionizing radiation than well-oxygenated tumors, while hyperthermia is particularly effective in hypoxic tumors.
Large solid tumors often contain hypoxic areas due to heterogeneous vascularization, making hyperthermia a useful addition to radiotherapy. The complementary effect of hyperthermia and radiotherapy is also because cells in the S-phase of the cell cycle are more sensitive to hyperthermia than the G1-phase, whereas cells are more resistant to radiotherapy in the S-phase.
Repair of DNA damage caused by radiotherapy is inhibited by hyperthermia. Hyperthermia also induces radiosensitization and chemosensitization. Furthermore, blood flow increases during hyperthermia improving tumor oxygenation and probably enhancing radiosensitivity. The increased blood flow also improves the uptake of cytostatics in tumor cells. Thus, the increased blood flow during hyperthermia is favorable for improving radiotherapy and chemotherapy effectiveness.
In clinical practice, we need to increase the temperature in a more or less circumscribed body region with tumor load. Treated volume ranges from a few cubic centimeters in case of thermoablation in lesions up to heating the whole body. Because of this, we need different types of applicators for each of the below-mentioned special cases. Thus, we can speak about different clinical modes of microwave hyperthermia.
First of all, we would like to offer an overview of the technical equipment needed for clinical applications of microwave thermotherapy in this chapter. Further, the main basic principles of EM field behavior inside the living biological system, selected from the point of view of physics related to microwave thermotherapy, will be mentioned. Moreover, we will provide the reader with references in literature, where detailed information on both physical and technical aspects of microwave thermotherapy (especially microwave hyperthermia) can be found.
According to ESHO guidelines following classification of different clinical modes of microwave hyperthermia (or thermotherapy in general) can be made:
Local hyperthermia – Medical indications for local hyperthermia include chest wall recurrences, superficial malignant melanoma lesions, and lymph node metastases of head and neck tumors—all of which are validated in prospective randomized studies. The basic physical and technical descriptions will be given in the following text.
Regional hyperthermia – Medical indications for regional hyperthermia include locally advanced and/or recurrent pelvis tumors, i.e., rectal carcinoma, cervical carcinoma, bladder carcinoma, prostate carcinoma, or soft tissue sarcoma. Some of these indications were validated in prospective studies. Basic physical and technical descriptions will be given in the following text.
Part-body hyperthermia – Heated volume of a body region such as the whole pelvis, the whole abdomen, or (if clinically desirable) the upper abdomen or lower thorax or others. Basic physical and technical descriptions will be given in the following text.
Whole-body hyperthermia (WBHT) –means to heat the whole body either up to 42°C for 60 minutes (so-called “Extreme WBHT”) or only 39.5–41°C for a longer time, e.g., 3 hours (so-called “Moderate WBHT” or “Fever-like WBHT”).
Thermoablation – is performed with thin laser applicators or radiofrequency electrodes of a few millimeters. It is a minimally invasive procedure in every case, i.e., the applicators must be implanted in the lesions under CT or MR guidance. Achieved temperatures are high (up to 90°C), but the thermal gradients are pretty steep, and the effective range is 1–2 cm (i.e., lesions with diameters of 3–4 cm are the limit using standard techniques). Liver metastases (numbers up to 4) are the most frequent indication. The procedures are typically performed under MR control.
Interstitial hyperthermia – an array of interstitial antennas or electrodes is implanted in inaccessible tumors, which might be located in deep or superficial tissues. The distance between the antennas must not exceed 1–2 cm, and therefore, lesions with diameters below 5 cm are suitable (in order to limit the number of puncturing tracks). From a physical point of view, we mostly want to create the best possible approximation through outward propagating spherical EM waves irradiated from each applicator. Thus, we can get the best approximation of the tumor dimensions and shape by dimensions and shape of the SAR distribution and thus, the best approximation of temperature distribution. Interstitial hyperthermia is an invasive procedure. Temperature measurements must be performed at the antennas and between them. In most systems, every single antenna is controlled by its generator. Dedicated systems have two or more segments per antenna or electrode controlled in phase and/or amplitude. Clinically interstitial hyperthermia has been applied for prostate carcinoma, recurrent breast cancer, and malignant brain tumors.
Endoluminal hyperthermia – uses natural orifices to position various kinds of endocavitary applicators (microwave, radiowave, ultrasound, etc.) in direct contact with a tumor. From the physical point of view, we mostly want to create the best possible approximation with the aid of an outward propagating cylindrical EM wave irradiated from the applicator. For physical reasons, the penetration depth around those endoluminal applicators is limited and of the order of the applicator’s diameter (in the centimeter range). Accessible tumors include esophageal carcinoma, prostate carcinoma, rectal and cervical carcinoma.
The term “Local hyperthermia” means superficial treatment, and typically the clinical range is up to 3–4 cm. It can be performed with so-called superficial applicators, e.g., based on EM waves in the lower part of the microwave frequency band (usually 434, 915, and 2450 MHz), ultrasound, and IR power. The technological base of EM wave applicators can be of different kinds: waveguides (water filled or with evanescent mode), microwave planar technology (e.g., patches and spirals), and according to results of this habilitation thesis, MTM applicators are very perspective as well.
From a physical point of view, we mostly want the superficial applicators to create the best possible approximation of a plane EM wave, which is the case of the deepest penetration of EM power into the area to be treated (at a given working frequency) and the best homogeneity of SAR distribution and thus, the best homogeneity of temperature distribution as well.
A system for local hyperthermia consists of a microwave (MW) power generator, an MW applicator for transfer of EM power into the treated area, see eq. (7) (tumor), a multichannel thermometer with several probes for temperature measurements in the tumor and its surroundings, and the main computer. See the schematics in Figure 2.
MW hyperthermia system schematics.
Invasive sensors then measure temperature, and according to it, MW power is being controlled in order to keep the temperature on a predetermined level.
The applicator is positioned upon the area to be treated and coupled to the tissue by a water bolus. The temperature and pressure of the water in the water bolus are possible to control, so it is possible to modify the temperature profile in the area to be treated.
In our discussion, it is essential to distinguish the following two important terms: “Depth of EM wave penetration” and “Depth of efficient treatment.” The second one can have different definitions for different clinical applications of thermotherapy treatments (i.e., hyperthermia, physiotherapy, and ablations). Here, we will work with the definition for hyperthermia only.
For the initial estimation of EM wave penetration into biological tissue, we can take a model for the behavior of amplitude of the plane wave in a lossy media.
where E is electrical field intensity, E0 is its value at the surface of biological tissue, z is the depth under the surface, and α means the attenuation constant of EM wave in lossy media.
The depth of the EM wave penetration
It can be seen that
In the case of microwave hyperthermia, the depth of the efficient treatment is given by the distribution of temperature in the treated area—it is formulated as a 25% decrease of the SAR value with respect to the maximum value of SAR inside the treated area. That guarantees quick and quality heating of the treated area from 37°C to at least 41°C. Suppose the maximum temperature in the tumor will be at the level of 45°C, then the depth of the efficient treatment depends on the following factors:
Depth of EM wave penetration – At a given working frequency and a given type of biological tissue.
EM field distribution in the aperture of the applicator – Usually, we are trying to create in the aperture of the applicator the distribution of EM field very similar to plane wave—thus, it is possible to accomplish the deepest penetration depth for the particular frequency and aperture dimensions.
Aperture size of the applicator – Bigger aperture size helps to approach better the EM field distribution inside the applicator aperture to the case of a plane wave. Very good results are expected if the aperture dimension size is comparable or bigger than a half wavelength at the operating frequency.
3D configuration of biological tissues in front of applicator aperture – Biological tissues can roughly be sorted into two categories: high or low water content. Tissues with high water content have higher attenuation than tissues with low water content. So, e.g., in the fat layer, there is EM wave less attenuated than in muscle tissue, and thus it penetrates deeper in fat than into muscle tissue.
Temperature of water in water bolus – This water can cool the surface of the area to be treated and thus improve the temperature profile inside this area.
There is a general rule for hyperthermia applicators optimization if we need to reach the maximal depth of efficient treatment and the best possible homogeneity of the temperature distribution inside the treated area. At least in the central part of their aperture, the distribution of the EM field should be very similar to plane wave; thus, it is possible to accomplish the deepest penetration depth for the particular frequency and aperture dimensions.
The term “Regional hyperthermia” means treating deep-seated tumors of the pelvis or lower extremities, etc. The so-called regional applicators can perform treatment, i.e., usually, an array of phase-controlled radiating elements typically working in the frequency range of 50–150 MHz. As radiating elements again, waveguides or dipoles are mostly being used. They surround the whole circumference; all possible directions are employed to deliver EM energy into the treated volume. The higher number of antennas and higher the frequency have the potential to control the heating 3D pattern. Several rings of antennas directed to the patient axis can be used to enable flexibility with respect to the anatomical structures for optimization.
A system for regional hyperthermia consists of a microwave (MW) or radiofrequency (RF) multichannel power generator (multiple power generators), an array of MW applicators for focusing EM power into the area to be treated (tumor), a multichannel thermometer with several probes for measurements of temperature in the tumor and its surroundings, and the main computer; see the schematics in Figure 3.
Schematics of microwave system for regional or part-body hyperthermia.
Applicators are positioned upon the area to be treated and coupled to the tissue by a water bolus. The water temperature in the water bolus is possible to control, so it is possible to modify the temperature profile in the area to be treated, like in the case of local hyperthermia. In the case of regional hyperthermia, the water temperature in the water bolus is usually below 10°C.
From a physical point of view, we mostly want to create the best possible approximation of a cylindrical or spherical EM wave irradiated from several (typically from 4 up to 12) single applicators situated around the patient. Superposition of the waves from these single applicators then creates inward propagating cylindrical or spherical waves, enabling the focus EM power in the area to be treated. Thus we can get the best approximation of the tumor dimensions and shape by dimensions and shape of the SAR distribution and thus, the best approximation of temperature distribution.
In discussed case, when we have a cylindrical phantom surrounded by several above mentioned applicators, then for the thermotherapy, the most important component of the EM field will be longitudinal component Ez, which in the discussed case can be expressed by equation.
where
As cylindrical agar phantom is mimicking muscle tissue, i.e., lossy medium, then the argument of Hankel function is a complex number. In Figure 3, there are three basic cases of temperature distribution inside an area treated by regional hyperthermia, which follows from the behavior of the Hankel function (see the narrow colored strips signed by letters
If the frequency is very low (from 27 to 80 MHz) and thus the depth of the penetration at this frequency is comparable to or bigger than the radius of the phantom
If the frequency is increased to 100 MHz or higher (i.e., attenuation constant is increasing), then we expect to approach the shape of the temperature distribution given by case
Case
Given examples of frequency bands are valid either for the human body of average dimensions or for agar phantom with similar dimensions and dielectric parameters with values near to values valid for muscle tissue.
Part-body hyperthermia is a technique derived from the regional approach and developed to heat a selected anatomical region in an extended manner up to 41–42°C under careful MR monitoring. From a physical point of view, we want mostly to create the best possible approximation of a spherical EM wave irradiated from several (typically from 12 up to 24) single applicators situated around the patient.
Superposition of the waves from these single applicators then creates inward propagating spherical waves enabling the focus EM power in the area to be treated. Thus, we can get the best approximation of the tumor dimensions and shape by dimensions and shape of the SAR distribution and thus, the best approximation of temperature distribution.
Due to safety reasons, the use of MR monitoring (to measure online temperature and perfusion) and a planning system is required at these higher power levels. Systems for part-body hyperthermia are called “hybrid systems” because they are based on the MR-compatible integration of a multiantenna applicator into an MR tomograph.
The most important effect from the point of view of microwave thermotherapy is the propagation of EM waves through the biological tissue to be treated. It can be classified as lossy dielectrics. So the power (energy) of propagating EM wave will be changed into thermal power (energy). For more details, see [1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20].
EM energy turns to heat, particularly due to the following mechanism: When the alternate field takes effect, vibrating electric particles lag behind the exciting intensity of the electric field; the current is not entirely in phase with electric field intensity. It is possible to describe this phase mathematically in a way that we virtually split up the movement of electrons into:
Component that follows electric field intensity.
Component that is in phase with the difference of potentials on electrodes.
The first component mentioned above determines the real part of permittivity
When the electromagnetic energy goes through the biological tissue, it is absorbed and turned into heat, resulting in a temperature increase of biological tissue within the irradiated area. Spatial distribution of temperature induced the way mentioned above (with respect to depth of EM wave penetration and depth of efficient treatment) depends on various factors.
The interaction of the EM field with biological tissue studied from a physical point of view is well described in several references, e.g. [1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20], so we do not need to go into the details here. When studying these interactions to be used in clinical applications of thermotherapy, then usually it is necessary to determine by calculations or measurements following 3D or 4D distributions:
The 3D spatial distribution of the values of the EM field main quantities (e.g., vector of electric field strength
The 3D spatial distribution of power
The 3D spatial distribution of specific absorption rate—the SAR [W/kg] indicates the EM energy absorbed in the biological tissue and, as shown by the unit, it is the power absorbed per 1 kg of tissue
where
Which can be further modified as
where
The 4D—i.e., spatial and time-dependent distribution of temperature
High-frequency electromagnetic fields can penetrate the human body and propagate through. During the propagation of EM waves through biological tissues, their energy is gradually absorbed and converted into heat, thereby increasing the temperature of the irradiated area. To such a wave, biological tissues behave as a lossy dielectric. In such a case, permittivity and permeability become to be complex numbers. The spatial distribution of temperature depends on many factors, the most important of which are:
The type of the EM wave (i.e., whether it is planar, cylindrical, or spherical).
Operating frequency determines the EM wavelength (i.e., penetration depth).
Spatial distribution of the biological tissue in the irradiated volume.
Dielectric and thermal characteristics of each tissue type in a certain area.
Blood flowing into the treated area.
The term treatment planning for clinical application of the thermotherapy means mathematical and experimental modeling of the effective treatment timing to determine the four-dimensional (4D) distribution of temperature (i.e., 3D in space + temperature behavior with respect to time) during the scheduled treatment (both within the treatment area and in its surroundings).
When preparing a particular type of clinical application, it is necessary to perform a series of experiments and model calculations to create a specific idea about the actual distribution of temperature (with respect to SAR) in the treated area. It is a highly complex problem that is not yet fully resolved. This is due to several factors, of which the most important may be considered:
highly inhomogeneous nature of the biological object—i.e., mainly irregular and complex definable spatial distribution of different types of biological tissues in the human body,
in practice, the usually unavailable precise description of the topology of the bloodstream, and particularly its response to external stimuli—i.e., mainly at an increasing temperature in the treatment area,
very “complicated” nature called near electromagnetic field emitted from the aperture thermotherapy applicators.
In the case of treatment planning, first, we need to do the calculation of SAR 3D distribution and after to do the calculation of the temperature 3D distribution. This distribution inside the treated area (heated by microwave energy
where
The possibilities of an analytical solution to this equation are limited to a few cases—e.g., the “one-dimensional” case of plane wave penetrating homogeneous phantom. Therefore, computers are to be used to solve this equation to obtain the temperature
In general, it is necessary to solve the time dependence of the temperature
The versatile option is to apply numerical methods using very powerful computers. The numerical solution then typically uses differential methods or finite element methods. The biggest problem then acts precisely, defining and modeling the bloodstream and its responses to cool or heat certain areas of the human body. The situation is further complicated dramatically by the topology of the heated area. The topology can be a good guess for subsurface treatment in clinical applications. However, the more complicated is the situation for deep regional heating when mapping the treated area requires a CT and/or MRI.
Noninvasive temperature monitoring of hyperthermia cancer treatment is one of the crucial points for its successful clinical applications. MRI is often discussed to be a prospective solution to this problem. However, it is a costly way (commercially available hyperthermia system controlled by MRI has a price above 1 million EUR). Because of that, cheaper solutions-based on, e.g., microwave or ultrasound technology, could be a convenient alternative to MRI temperature monitoring. Till now, microwave radiometers have been discussed for this purpose. Radiometers can measure the absolute value of temperature, but their spatial resolution is not sufficient. They integrate thermal noise from certain volumes, and thus, they indicate approximately average temperature inside this volume, so it may happen that the microwave radiometer will not identify existing hot spots or cold spots.
Our theoretical and experimental research work is focused now on microwave differential tomography (MDT). The Department of Biomedical Technology (the Czech Technical University in Prague) developed its own MDT system (see photo in Figure 4) in cooperation with Prof. Andrea Massa from Eledia Research Center (Trento, Italy). It seems realistic that the MDT methods can be used for 3D noninvasive temperature monitoring of the treated volume during thermotherapy in oncology. Existing suitable reconstruction algorithms, which allow quasi-real-time monitoring of changes of dielectric properties due to changes of temperature, were implemented. Reconstruction algorithms were tested on different 2D and 3D models. The obtained results using the Distorted Born Algorithm (DBA) and Born Algorithm (BA) were compared in terms of the algorithms’ ability to reconstruct the shape and position of the target and flatness of the obtained object function in regions without change in dielectric properties.
Photograph of an experimental system for research of MDT.
Our research work is oriented toward studies and developments of local external applicators working at 27, 70, 434, and 2450 MHz (see Figure 2). These applicators were used to treat deep-seated and/or superficial tumors (treatment depth from 2 up to 8 cm).
Now, following new trends in this field, we continue our research in the important directions of regional applicators (see Figure 3). Moreover, we are trying to implement new microwave technologies in the design of new hyperthermia applicators, e.g., applicators based on metamaterial technology. Our BioEM team with Prof. Paul Stauffer from Thomas Jefferson University Hospital in Philadelphia has developed such applicators. Research of MW thermotherapy systems and MW medical diagnostics is in Prague done in cooperation with Dept. of EM Field (Faculty of Electrical Engineering) and Dept. of Biomedical Technique (Faculty of Biomedical Engineering), both are part of the Czech Technical University in Prague. The most important technical activities in this field can be specified as:
Design of the applicators based on new MW technologies, i.e., development of new type applicators for more effective local, intracavitary, and regional treatment;
Development of treatment planning, i.e., mathematical and experimental modeling of the effective treatment;
Feasibility study of noninvasive temperature monitoring, e.g., microwave differential tomography or UWB radar technology;
MW medical diagnostics (e.g., MW differential tomography).
In Figure 5, there is an example of the calculated distribution temperature obtained by a matrix of a 3x2 MTM elements array. The highest temperature level is displayed here in red color, and the yellow color denotes the threshold therapeutic temperature of 41°C.
Calculation of the temperature distribution at 3.5 cm depth under the 3 × 2 MTM elements array. The yellow color denotes the threshold of the treated area.
In actual clinics, we need the treatment planning to create so-called phantoms of the patient body or at least phantoms of the area to be treated, see Figure 6a and b. In Figure 6a, there is an example of a homogeneous phantom; in Figure 6b then, there is an example of the anatomical phantom. The first one is suitable for verifying the fundamental behavior of the applicator; the second one then is needed for the 3D SAR and 3D temperature distribution during the treatment of the actual patient.
Example of a homogeneous (a) and the anatomical phantom (b).
In Figure 7, an example of SAR distribution is calculated for the case of the anatomical phantom. A very strong focus of MW power on a big tumor can be observed here.
Example of SAR distribution calculated for the case of anatomical phantom given in
As mentioned above, recently, there have been strong trends in research to apply microwave technology in medical diagnostics. Significant importance for the future can be identified for above all the following methods: microwave differential tomography, microwave diagnostic UWB radar, and microwave radiometry.
In Prague, the MDT is developed by a research group from the Dept. of Biomedical Technology in cooperation with Prof. Andrea Massa and his group from ELEDIA Research Center (University of Trento, Italy). Theoretical works are focused on a theory of differential microwave imaging (DMI) in quasi-real-time. Existing suitable reconstruction algorithms, namely Distorted Born Algorithm (DBA) and Born Algorithm (BA), which allow quasi-real-time monitoring of changes of dielectric properties due to changes of temperature, were implemented. They were applied and tested both numerically and experimentally within the feasibility studies.
These reconstruction algorithms were tested on numerical data from numerical 2D and 3D simulations; see Figure 8.
Numerical models for testing of reconstruction algorithms.
The below described results based on DBA and BA were compared in terms of the ability to reconstruct the shape and position of the target and flatness of the obtained object function in regions without change in dielectric properties. Influences of varying TSVD threshold values, number of voxels, calibration, and normalization were tested. BA with a low TSVD-threshold value leads to clear pictures of the difference in relative permittivity, but we lose information about the difference in conductivity. The described algorithms were tested with a sphere that was virtually homogeneously heated. The resulting pictures were not of the clear boundary of the so-called objective function: the predicted changes of object function are smooth, see Figures 9 and 10. Even if the implemented algorithms show several deficits, they represent state of the art and are therefore a suitable starting point in developing the combined MW system. Here described, the principle of noninvasive temperature monitoring, once it is commercially available, would mean a very significant improvement in quality assurance for hyperthermia treatment of oncological patients in actual clinics and for the comfort of their treatment as well.
Results of reconstruction on a 2D model.
Results of reconstruction on a 3D model.
In Figure 4, there is a photograph of the laboratory MDT system built at the Dept. of Biomedical Technique. In this case, it consists of eight bow-tie antennas, but we can go up to 24 antennas in total. Necessary MATLAB scripts for measurements automatization, data acquisition, and image reconstruction were implemented by us. We created numerical models for solving the forward problem, which is necessary for the reconstruction algorithms. A preliminary evaluation of the system based on measurement results was performed at the same time. It seems realistic that the DMI methods can be used for 3D noninvasive temperature monitoring of the treated volume during thermotherapy in oncology.
Currently, we study (by means of numerical simulations) the suitability of different types of antennas, e.g., their EM principle, dimensions, number, and geometrical configuration. We know that the main resolution limit of the described system is a low number of radiating elements. We plan to extend the system to the maximum possible number of antenna elements (i.e., up to 24). We believe there will be considerable improvement in the resolution.
Another prospective possibility of using the principle and technology of DMI is the rapid detection, identification, and classification of strokes (SDI), which would be essential for the quick, qualified decision of what kind of treatment is necessary to give to the stroke patient already in the ambulance when he/she is being transferred to the hospital. The Pioneer research group in this area is a team of Prof. Mikael Persson from Chalmers University in Goeteborg, Sweden.
Dr. Marko Helbig and Dr. Juergen Sachs from TU Ilmenau in Germany came up with the idea to use microwave UWB radar technology for noninvasive microwave imaging and/or noninvasive temperature monitoring. In Prague, they are followed by people from the Dept. of EM Field.
The detection of temperature change via UWB radar signal is based on the fact that the complex permittivity changes with temperature. We have shown that it is possible to detect these changes by UWB microwave radar. In our case, the antenna array comprises eight dipole antennas (21 x 11 mm). These antennas are excited by the UWB pulse in the frequency band 1–8 GHz. The values of relative permittivity and specific conductivity of all considered tissue temperatures (at starting temperature of 37°C) can be taken, e.g., from the IT’IS Foundation database.
We worked with an experimental antenna setup for UWB temperature change detection to be used in microwave hyperthermia treatment. Our numerical and laboratory models with implemented frequency and temperature dispersive parameters of biological tissues were used for a series of simulation purposes. The results from our numerical simulations show that it is possible to identify even very low changes in tumor permittivity caused by temperature change.
Our experiments with the homogeneous and nonhomogeneous phantoms have shown that we can detect even different temperature layers. From the reconstructed image, we can partially reconstruct the shape and position of the simulated inhomogeneity. The way to improve the chance for more accurate differential temperature reconstruction is in the higher number of antennas closer to the heated area utilization and in the attenuation correction improvement.
Research studies on the interactions between the EM field and biological systems have been the subject of high interest during the last decades. Here, we would like to give more details about such kind of research and obtained technical and biological results (i.e., basic description of implemented exposure systems). Two of our recent projects were oriented on the research of thermal effects of EM field (using either waveguide or array applicators). And the third one then on the research of nonthermal effects. Whole-body exposure chamber, operating at 900 MHz, was developed for small animals in the frame of this research project. The setup was designed with respect to homogeneity of induced EM field, elimination of external radiation, and exact determination of absorbed power. Further sufficient space for mice movement was taken into account. The whole-body exposure chamber with an anatomical mouse model was simulated by two different numerical methods, e.g., finite-difference-time-domain method (FDTD) and finite integration technique (FIT), and compared computed SAR values and its dosimetry results.
The major advantage of the system we will describe here is the capability of direct measurement of the whole-body averaged SAR, which is performed by analysis of measured scattering parameters. As the basic idea and principle of the discussed exposure chamber, a circular waveguide was chosen. The advantage of the waveguide structure is a perfect shielding of EM field generated either inside (in order to protect the operators) or generated outside the system (in order to eliminate interference caused by external EM fields). The circularly polarized wave TE11 is excited inside the exposure chamber with the aid of two monopoles that have mutually orthogonal orientations, and the distance between them is equal to one-fourth of the wavelength. Such circularly polarized wave provides relatively constant field coupling to each mouse regardless of its position, posture, or movement. The discussed exposure chamber is displayed in Figure 11.
Waveguide-type exposure chamber for animal experiments.
EM field distribution and impedance matching of the discussed exposure chamber were optimized and verified by 3D EM field simulators SEMCAD X resp. Sim4Life. Dimensions of the exposure chamber were calculated to use the desired frequency of operation and the volume needed to expose mice. The exposure chamber is made of a copper cylinder with dimensions of 1650 mm in length and 240 mm in diameter. It is terminated by matched loads at both ends (conical shape, 500 mm long, and made of RF absorbers). The reflection loss of the matched load is more than −20 dB at 900 MHz.
The exposed mice are kept in a cylindrical box that is made of Styrofoam. Styrofoam has a dielectric constant of 1.03, i.e., very close to that of air, and thus, the disturbance of exposure and measurements is negligible. The box provides space for two separated mice. Punctured slit-like holes are set on the cover and side of the box for air ventilation. In the study, the mice were held in the chamber only during RF exposures, and therefore, no food or drinking water was necessary.
For the survival of experimental animals inside the exposure chamber, it is important to create efficient ventilation, which will maintain a constant temperature and good air quality in the chamber. The air comes toward mice through the ventilation hole placed below the styrofoam box and flows toward the second opposite ventilation hole placed above the box.
To be able to evaluate the results of experiments with small animals (mice in our case), we need to specify appropriate dosimetry. It is the quantification of the magnitude and distribution of absorbed EM energy within biological objects that are exposed to EM fields. In the case of radiofrequency and microwave frequency bands, there is the dosimetric quantity, which is called SAR (i.e., specific absorption rate). It is defined as the rate at which energy is absorbed per unit mass. The SAR is determined and influenced not only by the incident EM waves but also by the electrical and geometric characteristics of the irradiated subject and objects nearby it. It is strongly related to the internal electric field strength E as well as to the electric conductivity σ and the density of tissues ρ as discussed above and as can be seen, reminded by the following equation.
Therefore, SAR is a suitable dosimetric parameter, even when a studied mechanism is determined to be “athermal.” SAR distributions are usually determined from measurements in animal tissues or from numerical calculations. It generally is difficult to measure the SAR directly in a living biological body, and therefore, dosimetry efforts are forced to rely on computer simulations mainly.
An anatomically based dielectric model of an experimental animal is essential for numerical dosimetry. It can be developed commonly from MRI or CT scans. In order to develop it, original gray-scale data must be interpreted into tissue types known as a process of segmentation. In our studies, the CT scans for mouse model development were obtained from the website: http://neuroimage.usc.edu/Digimouse_download.html. The mouse model has the resolution 0.1 mm, meaning voxel size 0.1 x 0.1 x 0.1 mm. Each voxel was assigned to one of 14 different tissue types, such as bone, muscle, brain, etc.
For dosimetry with the numerical voxel models, proper permittivity and conductivity values must be assigned to each tissue. The data from 10 MHz to 6 GHz, derived from 4-Cole-Cole extrapolation based on measurements for small animals, constitute the most widely accepted database for this information. The data are recommended by various international standardization organizations and can be accessed, e.g., from the website http://www.fcc.gov/fcc-bin/dielec.sh.
In order to verify and rely on numerical dosimetry results, the simulations of the exposure chamber were done in two different EM field simulators (based on two different numerical methods). Our choice was SEMCAD X, which uses the finite difference time domain (FDTD) method, and CST Microwave Studio, which uses the finite integration technique (FIT) method. We used these simulations to the determination of SAR distribution inside the mice during experiments.
Researchers from Medical Faculty in Pilsen, Charles University (Prof. František Vožeh, MD., Jan Barcal, MD.), did biological experiments with the aid of this exposure chamber. With the aim of whether EM exposure can increase the content of free radicals in the exposed tissue, a series of EM exposures to small animals (mice) was done. SAR level was at the level of 0.8 W/kg in the case of these experiments. Evaluation of preliminary results is displayed in Figure 12. It can be interpreted as a significantly increased content of free radicals being found.
Results of an animal experiment: in all four studied organ specimens (brain, heart, kidney, and liver), significantly increased content of free radicals was found.
This research was funded by Ministry of Education, Youth and Sports of the Czech Republic under Grant LTC19031, and the Student Grant Competition of the CTU, grant number SQS20/203/OHK4/3T/17.
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Buchholz",profilePictureURL:"https://mts.intechopen.com/storage/users/89438/images/6463_n.jpg",institutionString:null,institution:{name:"Loma Linda University",institutionURL:null,country:{name:"United States of America"}}}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null}]},subseriesFiltersForPublishedBooks:[{group:"subseries",caption:"Plant Physiology",value:13,count:1},{group:"subseries",caption:"Human Physiology",value:12,count:2},{group:"subseries",caption:"Cell Physiology",value:11,count:8}],publicationYearFilters:[{group:"publicationYear",caption:"2022",value:2022,count:1},{group:"publicationYear",caption:"2020",value:2020,count:4},{group:"publicationYear",caption:"2019",value:2019,count:5},{group:"publicationYear",caption:"2018",value:2018,count:1}],authors:{paginationCount:739,paginationItems:[{id:"158492",title:"Prof.",name:"Yusuf",middleName:null,surname:"Tutar",slug:"yusuf-tutar",fullName:"Yusuf Tutar",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/158492/images/system/158492.jpeg",biography:"Prof. Dr. Yusuf Tutar conducts his research at the Hamidiye Faculty of Pharmacy, Department of Basic Pharmaceutical Sciences, Division of Biochemistry, University of Health Sciences, Turkey. He is also a faculty member in the Molecular Oncology Program. He obtained his MSc and Ph.D. at Oregon State University and Texas Tech University, respectively. He pursued his postdoctoral studies at Rutgers University Medical School and the National Institutes of Health (NIH/NIDDK), USA. His research focuses on biochemistry, biophysics, genetics, molecular biology, and molecular medicine with specialization in the fields of drug design, protein structure-function, protein folding, prions, microRNA, pseudogenes, molecular cancer, epigenetics, metabolites, proteomics, genomics, protein expression, and characterization by spectroscopic and calorimetric methods.",institutionString:"University of Health Sciences",institution:null},{id:"180528",title:"Dr.",name:"Hiroyuki",middleName:null,surname:"Kagechika",slug:"hiroyuki-kagechika",fullName:"Hiroyuki Kagechika",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/180528/images/system/180528.jpg",biography:"Hiroyuki Kagechika received his bachelor’s degree and Ph.D. in Pharmaceutical Sciences from the University of Tokyo, Japan, where he served as an associate professor until 2004. He is currently a professor at the Institute of Biomaterials and Bioengineering (IBB), Tokyo Medical and Dental University (TMDU). From 2010 to 2012, he was the dean of the Graduate School of Biomedical Science. Since 2012, he has served as the vice dean of the Graduate School of Medical and Dental Sciences. He has been the director of the IBB since 2020. Dr. Kagechika’s major research interests are the medicinal chemistry of retinoids, vitamins D/K, and nuclear receptors. He has developed various compounds including a drug for acute promyelocytic leukemia.",institutionString:"Tokyo Medical and Dental University",institution:{name:"Tokyo Medical and Dental University",country:{name:"Japan"}}},{id:"94311",title:"Prof.",name:"Martins",middleName:"Ochubiojo",surname:"Ochubiojo Emeje",slug:"martins-ochubiojo-emeje",fullName:"Martins Ochubiojo Emeje",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/94311/images/system/94311.jpeg",biography:"Martins Emeje obtained a BPharm with distinction from Ahmadu Bello University, Nigeria, and an MPharm and Ph.D. from the University of Nigeria (UNN), where he received the best Ph.D. award and was enlisted as UNN’s “Face of Research.” He established the first nanomedicine center in Nigeria and was the pioneer head of the intellectual property and technology transfer as well as the technology innovation and support center. Prof. Emeje’s several international fellowships include the prestigious Raman fellowship. He has published more than 150 articles and patents. He is also the head of R&D at NIPRD and holds a visiting professor position at Nnamdi Azikiwe University, Nigeria. He has a postgraduate certificate in Project Management from Walden University, Minnesota, as well as a professional teaching certificate and a World Bank certification in Public Procurement. Prof. Emeje was a national chairman of academic pharmacists in Nigeria and the 2021 winner of the May & Baker Nigeria Plc–sponsored prize for professional service in research and innovation.",institutionString:"National Institute for Pharmaceutical Research and Development",institution:{name:"National Institute for Pharmaceutical Research and Development",country:{name:"Nigeria"}}},{id:"268659",title:"Ms.",name:"Xianquan",middleName:null,surname:"Zhan",slug:"xianquan-zhan",fullName:"Xianquan Zhan",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/268659/images/8143_n.jpg",biography:"Dr. Zhan received his undergraduate and graduate training in the fields of preventive medicine and epidemiology and statistics at the West China University of Medical Sciences in China during 1989 to 1999. He received his post-doctoral training in oncology and cancer proteomics for two years at the Cancer Research Institute of Human Medical University in China. In 2001, he went to the University of Tennessee Health Science Center (UTHSC) in USA, where he was a post-doctoral researcher and focused on mass spectrometry and cancer proteomics. Then, he was appointed as an Assistant Professor of Neurology, UTHSC in 2005. He moved to the Cleveland Clinic in USA as a Project Scientist/Staff in 2006 where he focused on the studies of eye disease proteomics and biomarkers. He returned to UTHSC as an Assistant Professor of Neurology in the end of 2007, engaging in proteomics and biomarker studies of lung diseases and brain tumors, and initiating the studies of predictive, preventive, and personalized medicine (PPPM) in cancer. In 2010, he was promoted to Associate Professor of Neurology, UTHSC. Currently, he is a Professor at Xiangya Hospital of Central South University in China, Fellow of Royal Society of Medicine (FRSM), the European EPMA National Representative in China, Regular Member of American Association for the Advancement of Science (AAAS), European Cooperation of Science and Technology (e-COST) grant evaluator, Associate Editors of BMC Genomics, BMC Medical Genomics, EPMA Journal, and Frontiers in Endocrinology, Executive Editor-in-Chief of Med One. He has\npublished 116 peer-reviewed research articles, 16 book chapters, 2 books, and 2 US patents. His current main research interest focuses on the studies of cancer proteomics and biomarkers, and the use of modern omics techniques and systems biology for PPPM in cancer, and on the development and use of 2DE-LC/MS for the large-scale study of human proteoforms.",institutionString:null,institution:{name:"Xiangya Hospital Central South University",country:{name:"China"}}},{id:"40482",title:null,name:"Rizwan",middleName:null,surname:"Ahmad",slug:"rizwan-ahmad",fullName:"Rizwan Ahmad",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/40482/images/system/40482.jpeg",biography:"Dr. Rizwan Ahmad is a University Professor and Coordinator, Quality and Development, College of Medicine, Imam Abdulrahman bin Faisal University, Saudi Arabia. Previously, he was Associate Professor of Human Function, Oman Medical College, Oman, and SBS University, Dehradun. Dr. Ahmad completed his education at Aligarh Muslim University, Aligarh. He has published several articles in peer-reviewed journals, chapters, and edited books. His area of specialization is free radical biochemistry and autoimmune diseases.",institutionString:"Imam Abdulrahman Bin Faisal University",institution:{name:"Imam Abdulrahman Bin Faisal University",country:{name:"Saudi Arabia"}}},{id:"41865",title:"Prof.",name:"Farid A.",middleName:null,surname:"Badria",slug:"farid-a.-badria",fullName:"Farid A. Badria",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/41865/images/system/41865.jpg",biography:"Farid A. Badria, Ph.D., is the recipient of several awards, including The World Academy of Sciences (TWAS) Prize for Public Understanding of Science; the World Intellectual Property Organization (WIPO) Gold Medal for best invention; Outstanding Arab Scholar, Kuwait; and the Khwarizmi International Award, Iran. He has 250 publications, 12 books, 20 patents, and several marketed pharmaceutical products to his credit. He continues to lead research projects on developing new therapies for liver, skin disorders, and cancer. Dr. Badria was listed among the world’s top 2% of scientists in medicinal and biomolecular chemistry in 2019 and 2020. He is a member of the Arab Development Fund, Kuwait; International Cell Research Organization–United Nations Educational, Scientific and Cultural Organization (ICRO–UNESCO), Chile; and UNESCO Biotechnology France",institutionString:"Mansoura University",institution:{name:"Mansoura University",country:{name:"Egypt"}}},{id:"329385",title:"Dr.",name:"Rajesh K.",middleName:"Kumar",surname:"Singh",slug:"rajesh-k.-singh",fullName:"Rajesh K. Singh",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/329385/images/system/329385.png",biography:"Dr. Singh received a BPharm (2003) and MPharm (2005) from Panjab University, Chandigarh, India, and a Ph.D. (2013) from Punjab Technical University (PTU), Jalandhar, India. He has more than sixteen years of teaching experience and has supervised numerous postgraduate and Ph.D. students. He has to his credit more than seventy papers in SCI- and SCOPUS-indexed journals, fifty-five conference proceedings, four books, six Best Paper Awards, and five projects from different government agencies. He is currently an editorial board member of eight international journals and a reviewer for more than fifty scientific journals. He received Top Reviewer and Excellent Peer Reviewer Awards from Publons in 2016 and 2017, respectively. He is also on the panel of The International Reviewer for reviewing research proposals for grants from the Royal Society. He also serves as a Publons Academy mentor and Bentham brand ambassador.",institutionString:"Punjab Technical University",institution:{name:"Punjab Technical University",country:{name:"India"}}},{id:"142388",title:"Dr.",name:"Thiago",middleName:"Gomes",surname:"Gomes Heck",slug:"thiago-gomes-heck",fullName:"Thiago Gomes Heck",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/142388/images/7259_n.jpg",biography:null,institutionString:null,institution:{name:"Universidade Regional do Noroeste do Estado do Rio Grande do Sul",country:{name:"Brazil"}}},{id:"336273",title:"Assistant Prof.",name:"Janja",middleName:null,surname:"Zupan",slug:"janja-zupan",fullName:"Janja Zupan",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/336273/images/14853_n.jpeg",biography:"Janja Zupan graduated in 2005 at the Department of Clinical Biochemistry (superviser prof. dr. Janja Marc) in the field of genetics of osteoporosis. Since November 2009 she is working as a Teaching Assistant at the Faculty of Pharmacy, Department of Clinical Biochemistry. In 2011 she completed part of her research and PhD work at Institute of Genetics and Molecular Medicine, University of Edinburgh. She finished her PhD entitled The influence of the proinflammatory cytokines on the RANK/RANKL/OPG in bone tissue of osteoporotic and osteoarthritic patients in 2012. From 2014-2016 she worked at the Institute of Biomedical Sciences, University of Aberdeen as a postdoctoral research fellow on UK Arthritis research project where she gained knowledge in mesenchymal stem cells and regenerative medicine. She returned back to University of Ljubljana, Faculty of Pharmacy in 2016. She is currently leading project entitled Mesenchymal stem cells-the keepers of tissue endogenous regenerative capacity facing up to aging of the musculoskeletal system funded by Slovenian Research Agency.",institutionString:null,institution:{name:"University of Ljubljana",country:{name:"Slovenia"}}},{id:"357453",title:"Dr.",name:"Radheshyam",middleName:null,surname:"Maurya",slug:"radheshyam-maurya",fullName:"Radheshyam Maurya",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/357453/images/16535_n.jpg",biography:null,institutionString:null,institution:{name:"University of Hyderabad",country:{name:"India"}}},{id:"418340",title:"Dr.",name:"Jyotirmoi",middleName:null,surname:"Aich",slug:"jyotirmoi-aich",fullName:"Jyotirmoi Aich",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y000038Ugi5QAC/Profile_Picture_2022-04-15T07:48:28.png",biography:"Biotechnologist with 15 years of research including 6 years of teaching experience. Demonstrated record of scientific achievements through consistent publication record (H index = 13, with 874 citations) in high impact journals such as Nature Communications, Oncotarget, Annals of Oncology, PNAS, and AJRCCM, etc. Strong research professional with a post-doctorate from ACTREC where I gained experimental oncology experience in clinical settings and a doctorate from IGIB where I gained expertise in asthma pathophysiology. A well-trained biotechnologist with diverse experience on the bench across different research themes ranging from asthma to cancer and other infectious diseases. An individual with a strong commitment and innovative mindset. Have the ability to work on diverse projects such as regenerative and molecular medicine with an overall mindset of improving healthcare.",institutionString:"DY Patil Deemed to Be University",institution:null},{id:"349288",title:"Prof.",name:"Soumya",middleName:null,surname:"Basu",slug:"soumya-basu",fullName:"Soumya Basu",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y000035QxIDQA0/Profile_Picture_2022-04-15T07:47:01.jpg",biography:"Soumya Basu, Ph.D., is currently working as an Associate Professor at Dr. D. Y. Patil Biotechnology and Bioinformatics Institute, Dr. D. Y. Patil Vidyapeeth, Pune, Maharashtra, India. With 16+ years of trans-disciplinary research experience in Drug Design, development, and pre-clinical validation; 20+ research article publications in journals of repute, 9+ years of teaching experience, trained with cross-disciplinary education, Dr. Basu is a life-long learner and always thrives for new challenges.\r\nHer research area is the design and synthesis of small molecule partial agonists of PPAR-γ in lung cancer. She is also using artificial intelligence and deep learning methods to understand the exosomal miRNA’s role in cancer metastasis. Dr. Basu is the recipient of many awards including the Early Career Research Award from the Department of Science and Technology, Govt. of India. She is a reviewer of many journals like Molecular Biology Reports, Frontiers in Oncology, RSC Advances, PLOS ONE, Journal of Biomolecular Structure & Dynamics, Journal of Molecular Graphics and Modelling, etc. She has edited and authored/co-authored 21 journal papers, 3 book chapters, and 15 abstracts. She is a Board of Studies member at her university. She is a life member of 'The Cytometry Society”-in India and 'All India Cell Biology Society”- in India.",institutionString:"Dr. D.Y. Patil Vidyapeeth, Pune",institution:{name:"Dr. D.Y. Patil Vidyapeeth, Pune",country:{name:"India"}}},{id:"354817",title:"Dr.",name:"Anubhab",middleName:null,surname:"Mukherjee",slug:"anubhab-mukherjee",fullName:"Anubhab Mukherjee",position:null,profilePictureURL:"https://intech-files.s3.amazonaws.com/0033Y0000365PbRQAU/ProfilePicture%202022-04-15%2005%3A11%3A18.480",biography:"A former member of Laboratory of Nanomedicine, Brigham and Women’s Hospital, Harvard University, Boston, USA, Dr. Anubhab Mukherjee is an ardent votary of science who strives to make an impact in the lives of those afflicted with cancer and other chronic/acute ailments. He completed his Ph.D. from CSIR-Indian Institute of Chemical Technology, Hyderabad, India, having been skilled with RNAi, liposomal drug delivery, preclinical cell and animal studies. He pursued post-doctoral research at College of Pharmacy, Health Science Center, Texas A & M University and was involved in another postdoctoral research at Department of Translational Neurosciences and Neurotherapeutics, John Wayne Cancer Institute, Santa Monica, California. In 2015, he worked in Harvard-MIT Health Sciences & Technology as a visiting scientist. He has substantial experience in nanotechnology-based formulation development and successfully served various Indian organizations to develop pharmaceuticals and nutraceutical products. He is an inventor in many US patents and an author in many peer-reviewed articles, book chapters and books published in various media of international repute. Dr. Mukherjee is currently serving as Principal Scientist, R&D at Esperer Onco Nutrition (EON) Pvt. Ltd. and heads the Hyderabad R&D center of the organization.",institutionString:"Esperer Onco Nutrition Pvt Ltd.",institution:null},{id:"319365",title:"Assistant Prof.",name:"Manash K.",middleName:null,surname:"Paul",slug:"manash-k.-paul",fullName:"Manash K. Paul",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/319365/images/system/319365.png",biography:"Manash K. Paul is a Principal Investigator and Scientist at the University of California Los Angeles. He has contributed significantly to the fields of stem cell biology, regenerative medicine, and lung cancer. His research focuses on various signaling processes involved in maintaining stem cell homeostasis during the injury-repair process, deciphering lung stem cell niche, pulmonary disease modeling, immuno-oncology, and drug discovery. He is currently investigating the role of extracellular vesicles in premalignant lung cell migration and detecting the metastatic phenotype of lung cancer via machine-learning-based analyses of exosomal signatures. Dr. Paul has published in more than fifty peer-reviewed international journals and is highly cited. He is the recipient of many awards, including the UCLA Vice Chancellor’s award, a senior member of the Institute of Electrical and Electronics Engineers (IEEE), and an editorial board member for several international journals.",institutionString:"University of California Los Angeles",institution:{name:"University of California Los Angeles",country:{name:"United States of America"}}},{id:"311457",title:"Dr.",name:"Júlia",middleName:null,surname:"Scherer Santos",slug:"julia-scherer-santos",fullName:"Júlia Scherer Santos",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/311457/images/system/311457.jpg",biography:"Dr. Júlia Scherer Santos works in the areas of cosmetology, nanotechnology, pharmaceutical technology, beauty, and aesthetics. Dr. Santos also has experience as a professor of graduate courses. Graduated in Pharmacy, specialization in Cosmetology and Cosmeceuticals applied to aesthetics, specialization in Aesthetic and Cosmetic Health, and a doctorate in Pharmaceutical Nanotechnology. Teaching experience in Pharmacy and Aesthetics and Cosmetics courses. She works mainly on the following subjects: nanotechnology, cosmetology, pharmaceutical technology, aesthetics.",institutionString:"Universidade Federal de Juiz de Fora",institution:{name:"Universidade Federal de Juiz de Fora",country:{name:"Brazil"}}},{id:"219081",title:"Dr.",name:"Abdulsamed",middleName:null,surname:"Kükürt",slug:"abdulsamed-kukurt",fullName:"Abdulsamed Kükürt",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/219081/images/system/219081.png",biography:"Dr. Kükürt graduated from Uludağ University in Turkey. He started his academic career as a Research Assistant in the Department of Biochemistry at Kafkas University. In 2019, he completed his Ph.D. program in the Department of Biochemistry at the Institute of Health Sciences. He is currently working at the Department of Biochemistry, Kafkas University. He has 27 published research articles in academic journals, 11 book chapters, and 37 papers. He took part in 10 academic projects. He served as a reviewer for many articles. He still serves as a member of the review board in many academic journals. He is currently working on the protective activity of phenolic compounds in disorders associated with oxidative stress and inflammation.",institutionString:null,institution:{name:"Kafkas University",country:{name:"Turkey"}}},{id:"178366",title:"Dr.",name:"Volkan",middleName:null,surname:"Gelen",slug:"volkan-gelen",fullName:"Volkan Gelen",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/178366/images/system/178366.jpg",biography:"Volkan Gelen is a Physiology specialist who received his veterinary degree from Kafkas University in 2011. Between 2011-2015, he worked as an assistant at Atatürk University, Faculty of Veterinary Medicine, Department of Physiology. In 2016, he joined Kafkas University, Faculty of Veterinary Medicine, Department of Physiology as an assistant professor. Dr. Gelen has been engaged in various academic activities at Kafkas University since 2016. There he completed 5 projects and has 3 ongoing projects. He has 60 articles published in scientific journals and 20 poster presentations in scientific congresses. His research interests include physiology, endocrine system, cancer, diabetes, cardiovascular system diseases, and isolated organ bath system studies.",institutionString:"Kafkas University",institution:{name:"Kafkas University",country:{name:"Turkey"}}},{id:"418963",title:"Dr.",name:"Augustine Ododo",middleName:"Augustine",surname:"Osagie",slug:"augustine-ododo-osagie",fullName:"Augustine Ododo Osagie",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/418963/images/16900_n.jpg",biography:"Born into the family of Osagie, a prince of the Benin Kingdom. I am currently an academic in the Department of Medical Biochemistry, University of Benin. Part of the duties are to teach undergraduate students and conduct academic research.",institutionString:null,institution:{name:"University of Benin",country:{name:"Nigeria"}}},{id:"192992",title:"Prof.",name:"Shagufta",middleName:null,surname:"Perveen",slug:"shagufta-perveen",fullName:"Shagufta Perveen",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/192992/images/system/192992.png",biography:"Prof. Shagufta Perveen is a Distinguish Professor in the Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia. Dr. Perveen has acted as the principal investigator of major research projects funded by the research unit of King Saud University. She has more than ninety original research papers in peer-reviewed journals of international repute to her credit. She is a fellow member of the Royal Society of Chemistry UK and the American Chemical Society of the United States.",institutionString:"King Saud University",institution:{name:"King Saud University",country:{name:"Saudi Arabia"}}},{id:"49848",title:"Dr.",name:"Wen-Long",middleName:null,surname:"Hu",slug:"wen-long-hu",fullName:"Wen-Long Hu",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/49848/images/system/49848.jpg",biography:"Wen-Long Hu is Chief of the Division of Acupuncture, Department of Chinese Medicine at Kaohsiung Chang Gung Memorial Hospital, as well as an adjunct associate professor at Fooyin University and Kaohsiung Medical University. Wen-Long is President of Taiwan Traditional Chinese Medicine Medical Association. He has 28 years of experience in clinical practice in laser acupuncture therapy and 34 years in acupuncture. He is an invited speaker for lectures and workshops in laser acupuncture at many symposiums held by medical associations. He owns the patent for herbal preparation and producing, and for the supercritical fluid-treated needle. Dr. Hu has published three books, 12 book chapters, and more than 30 papers in reputed journals, besides serving as an editorial board member of repute.",institutionString:"Kaohsiung Chang Gung Memorial Hospital",institution:{name:"Kaohsiung Chang Gung Memorial Hospital",country:{name:"Taiwan"}}},{id:"298472",title:"Prof.",name:"Andrey V.",middleName:null,surname:"Grechko",slug:"andrey-v.-grechko",fullName:"Andrey V. Grechko",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/298472/images/system/298472.png",biography:"Andrey Vyacheslavovich Grechko, Ph.D., Professor, is a Corresponding Member of the Russian Academy of Sciences. He graduated from the Semashko Moscow Medical Institute (Semashko National Research Institute of Public Health) with a degree in Medicine (1998), the Clinical Department of Dermatovenerology (2000), and received a second higher education in Psychology (2009). Professor A.V. Grechko held the position of Сhief Physician of the Central Clinical Hospital in Moscow. He worked as a professor at the faculty and was engaged in scientific research at the Medical University. Starting in 2013, he has been the initiator of the creation of the Federal Scientific and Clinical Center for Intensive Care and Rehabilitology, Moscow, Russian Federation, where he also serves as Director since 2015. 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Fungal infectious illness prevalence and prognosis are determined by the exposure between fungi and host, host immunological state, fungal virulence, and early and accurate diagnosis and treatment. \r\nPatients with both congenital and acquired immunodeficiency are more likely to be infected with opportunistic mycosis. Fungal infectious disease outbreaks are common during the post- disaster rebuilding era, which is characterised by high population density, migration, and poor health and medical conditions.\r\nSystemic or local fungal infection is mainly associated with the fungi directly inhaled or inoculated in the environment during the disaster. The most common fungal infection pathways are human to human (anthropophilic), animal to human (zoophilic), and environment to human (soilophile). Diseases are common as a result of widespread exposure to pathogenic fungus dispersed into the environment. \r\nFungi that are both common and emerging are intertwined. In Southeast Asia, for example, Talaromyces marneffei is an important pathogenic thermally dimorphic fungus that causes systemic mycosis. Widespread fungal infections with complicated and variable clinical manifestations, such as Candida auris infection resistant to several antifungal medicines, Covid-19 associated with Trichoderma, and terbinafine resistant dermatophytosis in India, are among the most serious disorders. \r\nInappropriate local or systemic use of glucocorticoids, as well as their immunosuppressive effects, may lead to changes in fungal infection spectrum and clinical characteristics. Hematogenous candidiasis is a worrisome issue that affects people all over the world, particularly ICU patients. CARD9 deficiency and fungal infection have been major issues in recent years. Invasive aspergillosis is associated with a significant death rate. Special attention should be given to endemic fungal infections, identification of important clinical fungal infections advanced in yeasts, filamentous fungal infections, skin mycobiome and fungal genomes, and immunity to fungal infections.\r\nIn addition, endemic fungal diseases or uncommon fungal infections caused by Mucor irregularis, dermatophytosis, Malassezia, cryptococcosis, chromoblastomycosis, coccidiosis, blastomycosis, histoplasmosis, sporotrichosis, and other fungi, should be monitored. \r\nThis topic includes the research progress on the etiology and pathogenesis of fungal infections, new methods of isolation and identification, rapid detection, drug sensitivity testing, new antifungal drugs, schemes and case series reports. It will provide significant opportunities and support for scientists, clinical doctors, mycologists, antifungal drug researchers, public health practitioners, and epidemiologists from all over the world to share new research, ideas and solutions to promote the development and progress of medical mycology.",coverUrl:"https://cdn.intechopen.com/series_topics/covers/4.jpg",keywords:"Emerging Fungal Pathogens, Invasive Infections, Epidemiology, Cell Membrane, Fungal Virulence, Diagnosis, Treatment"},{id:"5",title:"Parasitic Infectious Diseases",scope:"Parasitic diseases have evolved alongside their human hosts. In many cases, these diseases have adapted so well that they have developed efficient resilience methods in the human host and can live in the host for years. Others, particularly some blood parasites, can cause very acute diseases and are responsible for millions of deaths yearly. Many parasitic diseases are classified as neglected tropical diseases because they have received minimal funding over recent years and, in many cases, are under-reported despite the critical role they play in morbidity and mortality among human and animal hosts. The current topic, Parasitic Infectious Diseases, in the Infectious Diseases Series aims to publish studies on the systematics, epidemiology, molecular biology, genomics, pathogenesis, genetics, and clinical significance of parasitic diseases from blood borne to intestinal parasites as well as zoonotic parasites. We hope to cover all aspects of parasitic diseases to provide current and relevant research data on these very important diseases. In the current atmosphere of the Coronavirus pandemic, communities around the world, particularly those in different underdeveloped areas, are faced with the growing challenges of the high burden of parasitic diseases. At the same time, they are faced with the Covid-19 pandemic leading to what some authors have called potential syndemics that might worsen the outcome of such infections. Therefore, it is important to conduct studies that examine parasitic infections in the context of the coronavirus pandemic for the benefit of all communities to help foster more informed decisions for the betterment of human and animal health.",coverUrl:"https://cdn.intechopen.com/series_topics/covers/5.jpg",keywords:"Blood Borne Parasites, Intestinal Parasites, Protozoa, Helminths, Arthropods, Water Born Parasites, Epidemiology, Molecular Biology, Systematics, Genomics, Proteomics, Ecology"},{id:"6",title:"Viral Infectious Diseases",scope:"The Viral Infectious Diseases Book Series aims to provide a comprehensive overview of recent research trends and discoveries in various viral infectious diseases emerging around the globe. The emergence of any viral disease is hard to anticipate, which often contributes to death. A viral disease can be defined as an infectious disease that has recently appeared within a population or exists in nature with the rapid expansion of incident or geographic range. This series will focus on various crucial factors related to emerging viral infectious diseases, including epidemiology, pathogenesis, host immune response, clinical manifestations, diagnosis, treatment, and clinical recommendations for managing viral infectious diseases, highlighting the recent issues with future directions for effective therapeutic strategies.",coverUrl:"https://cdn.intechopen.com/series_topics/covers/6.jpg",keywords:"Novel Viruses, Virus Transmission, Virus Evolution, Molecular Virology, Control and Prevention, Virus-host Interaction"}],annualVolumeBook:{},thematicCollection:[{type:"book",id:"11672",title:"Chemokines Updates",subtitle:null,isOpenForSubmission:!0,hash:"c00855833476a514d37abf7c846e16e9",slug:null,bookSignature:"Prof. Murat Şentürk",coverURL:"https://cdn.intechopen.com/books/images_new/11672.jpg",editedByType:null,submissionDeadline:"May 6th 2022",editors:[{id:"14794",title:"Prof.",name:"Murat",middleName:null,surname:"Şentürk",slug:"murat-senturk",fullName:"Murat Şentürk",profilePictureURL:"https://mts.intechopen.com/storage/users/14794/images/system/14794.jpeg",biography:"Dr. Murat Şentürk obtained a baccalaureate degree in Chemistry in 2002, a master’s degree in Biochemistry in 2006, and a doctorate degree in Biochemistry in 2009 from Atatürk University, Turkey. Dr. Şentürk currently works as an professor of Biochemistry in the Department of Basic Pharmacy Sciences, Faculty of Pharmacy, Ağri Ibrahim Cecen University, Turkey. \nDr. Şentürk published over 120 scientific papers, reviews, and book chapters and presented several conferences to scientists. \nHis research interests span enzyme inhibitor or activator, protein expression, purification and characterization, drug design and synthesis, toxicology, and pharmacology. \nHis research work has focused on neurodegenerative diseases and cancer treatment. Dr. Şentürk serves as the editorial board member of several international journals.",institutionString:"Ağrı İbrahim Çeçen University",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"2",totalChapterViews:"0",totalEditedBooks:"1",institution:{name:"Ağrı İbrahim Çeçen University",institutionURL:null,country:{name:"Turkey"}}}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null}],selectedSeries:{title:"Infectious Diseases",id:"6"},selectedSubseries:null},seriesLanding:{item:{id:"11",title:"Biochemistry",doi:"10.5772/intechopen.72877",issn:"2632-0983",scope:"Biochemistry, the study of chemical transformations occurring within living organisms, impacts all areas of life sciences, from molecular crystallography and genetics to ecology, medicine, and population biology. Biochemistry examines macromolecules - proteins, nucleic acids, carbohydrates, and lipids – and their building blocks, structures, functions, and interactions. Much of biochemistry is devoted to enzymes, proteins that catalyze chemical reactions, enzyme structures, mechanisms of action and their roles within cells. Biochemistry also studies small signaling molecules, coenzymes, inhibitors, vitamins, and hormones, which play roles in life processes. Biochemical experimentation, besides coopting classical chemistry methods, e.g., chromatography, adopted new techniques, e.g., X-ray diffraction, electron microscopy, NMR, radioisotopes, and developed sophisticated microbial genetic tools, e.g., auxotroph mutants and their revertants, fermentation, etc. More recently, biochemistry embraced the ‘big data’ omics systems. Initial biochemical studies have been exclusively analytic: dissecting, purifying, and examining individual components of a biological system; in the apt words of Efraim Racker (1913 –1991), “Don’t waste clean thinking on dirty enzymes.” Today, however, biochemistry is becoming more agglomerative and comprehensive, setting out to integrate and describe entirely particular biological systems. The ‘big data’ metabolomics can define the complement of small molecules, e.g., in a soil or biofilm sample; proteomics can distinguish all the comprising proteins, e.g., serum; metagenomics can identify all the genes in a complex environment, e.g., the bovine rumen. This Biochemistry Series will address the current research on biomolecules and the emerging trends with great promise.",coverUrl:"https://cdn.intechopen.com/series/covers/11.jpg",latestPublicationDate:"June 29th, 2022",hasOnlineFirst:!0,numberOfOpenTopics:4,numberOfPublishedChapters:318,numberOfPublishedBooks:32,editor:{id:"31610",title:"Dr.",name:"Miroslav",middleName:null,surname:"Blumenberg",fullName:"Miroslav Blumenberg",profilePictureURL:"https://mts.intechopen.com/storage/users/31610/images/system/31610.jpg",biography:"Miroslav Blumenberg, Ph.D., was born in Subotica and received his BSc in Belgrade, Yugoslavia. He completed his Ph.D. at MIT in Organic Chemistry; he followed up his Ph.D. with two postdoctoral study periods at Stanford University. Since 1983, he has been a faculty member of the RO Perelman Department of Dermatology, NYU School of Medicine, where he is codirector of a training grant in cutaneous biology. Dr. Blumenberg’s research is focused on the epidermis, expression of keratin genes, transcription profiling, keratinocyte differentiation, inflammatory diseases and cancers, and most recently the effects of the microbiome on the skin. He has published more than 100 peer-reviewed research articles and graduated numerous Ph.D. and postdoctoral students.",institutionString:null,institution:{name:"New York University Langone Medical Center",institutionURL:null,country:{name:"United States of America"}}},subseries:[{id:"14",title:"Cell and Molecular Biology",keywords:"Omics (Transcriptomics; Proteomics; Metabolomics), Molecular Biology, Cell Biology, Signal Transduction and Regulation, Cell Growth and Differentiation, Apoptosis, Necroptosis, Ferroptosis, Autophagy, Cell Cycle, Macromolecules and Complexes, Gene Expression",scope:"The Cell and Molecular Biology topic within the IntechOpen Biochemistry Series aims to rapidly publish contributions on all aspects of cell and molecular biology, including aspects related to biochemical and genetic research (not only in humans but all living beings). We encourage the submission of manuscripts that provide novel and mechanistic insights that report significant advances in the fields. Topics include, but are not limited to: Advanced techniques of cellular and molecular biology (Molecular methodologies, imaging techniques, and bioinformatics); Biological activities at the molecular level; Biological processes of cell functions, cell division, senescence, maintenance, and cell death; Biomolecules interactions; Cancer; Cell biology; Chemical biology; Computational biology; Cytochemistry; Developmental biology; Disease mechanisms and therapeutics; DNA, and RNA metabolism; Gene functions, genetics, and genomics; Genetics; Immunology; Medical microbiology; Molecular biology; Molecular genetics; Molecular processes of cell and organelle dynamics; Neuroscience; Protein biosynthesis, degradation, and functions; Regulation of molecular interactions in a cell; Signalling networks and system biology; Structural biology; Virology and microbiology.",annualVolume:11410,isOpenForSubmission:!0,coverUrl:"https://cdn.intechopen.com/series_topics/covers/14.jpg",editor:{id:"165627",title:"Dr.",name:"Rosa María",middleName:null,surname:"Martínez-Espinosa",fullName:"Rosa María Martínez-Espinosa",profilePictureURL:"https://mts.intechopen.com/storage/users/165627/images/system/165627.jpeg",institutionString:null,institution:{name:"University of Alicante",institutionURL:null,country:{name:"Spain"}}},editorTwo:null,editorThree:null,editorialBoard:[{id:"79367",title:"Dr.",name:"Ana Isabel",middleName:null,surname:"Flores",fullName:"Ana Isabel Flores",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRpIOQA0/Profile_Picture_1632418099564",institutionString:null,institution:{name:"Hospital Universitario 12 De Octubre",institutionURL:null,country:{name:"Spain"}}},{id:"328234",title:"Ph.D.",name:"Christian",middleName:null,surname:"Palavecino",fullName:"Christian Palavecino",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y000030DhEhQAK/Profile_Picture_1628835318625",institutionString:null,institution:{name:"Central University of Chile",institutionURL:null,country:{name:"Chile"}}},{id:"186585",title:"Dr.",name:"Francisco Javier",middleName:null,surname:"Martin-Romero",fullName:"Francisco Javier Martin-Romero",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bSB3HQAW/Profile_Picture_1631258137641",institutionString:null,institution:{name:"University of Extremadura",institutionURL:null,country:{name:"Spain"}}}]},{id:"15",title:"Chemical Biology",keywords:"Phenolic Compounds, Essential Oils, Modification of Biomolecules, Glycobiology, Combinatorial Chemistry, Therapeutic peptides, Enzyme Inhibitors",scope:"Chemical biology spans the fields of chemistry and biology involving the application of biological and chemical molecules and techniques. In recent years, the application of chemistry to biological molecules has gained significant interest in medicinal and pharmacological studies. This topic will be devoted to understanding the interplay between biomolecules and chemical compounds, their structure and function, and their potential applications in related fields. Being a part of the biochemistry discipline, the ideas and concepts that have emerged from Chemical Biology have affected other related areas. This topic will closely deal with all emerging trends in this discipline.",annualVolume:11411,isOpenForSubmission:!0,coverUrl:"https://cdn.intechopen.com/series_topics/covers/15.jpg",editor:{id:"441442",title:"Dr.",name:"Şükrü",middleName:null,surname:"Beydemir",fullName:"Şükrü Beydemir",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y00003GsUoIQAV/Profile_Picture_1634557147521",institutionString:null,institution:{name:"Anadolu University",institutionURL:null,country:{name:"Turkey"}}},editorTwo:{id:"13652",title:"Prof.",name:"Deniz",middleName:null,surname:"Ekinci",fullName:"Deniz Ekinci",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002aYLT1QAO/Profile_Picture_1634557223079",institutionString:null,institution:{name:"Ondokuz Mayıs University",institutionURL:null,country:{name:"Turkey"}}},editorThree:null,editorialBoard:[{id:"219081",title:"Dr.",name:"Abdulsamed",middleName:null,surname:"Kükürt",fullName:"Abdulsamed Kükürt",profilePictureURL:"https://mts.intechopen.com/storage/users/219081/images/system/219081.png",institutionString:null,institution:{name:"Kafkas University",institutionURL:null,country:{name:"Turkey"}}},{id:"241413",title:"Dr.",name:"Azhar",middleName:null,surname:"Rasul",fullName:"Azhar Rasul",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRT1oQAG/Profile_Picture_1635251978933",institutionString:null,institution:{name:"Government College University, Faisalabad",institutionURL:null,country:{name:"Pakistan"}}},{id:"178316",title:"Ph.D.",name:"Sergey",middleName:null,surname:"Sedykh",fullName:"Sergey Sedykh",profilePictureURL:"https://mts.intechopen.com/storage/users/178316/images/system/178316.jfif",institutionString:null,institution:{name:"Novosibirsk State University",institutionURL:null,country:{name:"Russia"}}}]},{id:"17",title:"Metabolism",keywords:"Biomolecules Metabolism, Energy Metabolism, Metabolic Pathways, Key Metabolic Enzymes, Metabolic Adaptation",scope:"Metabolism is frequently defined in biochemistry textbooks as the overall process that allows living systems to acquire and use the free energy they need for their vital functions or the chemical processes that occur within a living organism to maintain life. Behind these definitions are hidden all the aspects of normal and pathological functioning of all processes that the topic ‘Metabolism’ will cover within the Biochemistry Series. Thus all studies on metabolism will be considered for publication.",annualVolume:11413,isOpenForSubmission:!0,coverUrl:"https://cdn.intechopen.com/series_topics/covers/17.jpg",editor:{id:"138626",title:"Dr.",name:"Yannis",middleName:null,surname:"Karamanos",fullName:"Yannis Karamanos",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002g6Jv2QAE/Profile_Picture_1629356660984",institutionString:null,institution:{name:"Artois University",institutionURL:null,country:{name:"France"}}},editorTwo:null,editorThree:null,editorialBoard:[{id:"243049",title:"Dr.",name:"Anca",middleName:null,surname:"Pantea Stoian",fullName:"Anca Pantea Stoian",profilePictureURL:"https://mts.intechopen.com/storage/users/243049/images/system/243049.jpg",institutionString:null,institution:{name:"Carol Davila University of Medicine and Pharmacy",institutionURL:null,country:{name:"Romania"}}},{id:"203824",title:"Dr.",name:"Attilio",middleName:null,surname:"Rigotti",fullName:"Attilio Rigotti",profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institutionString:null,institution:{name:"Pontifical Catholic University of Chile",institutionURL:null,country:{name:"Chile"}}},{id:"300470",title:"Dr.",name:"Yanfei (Jacob)",middleName:null,surname:"Qi",fullName:"Yanfei (Jacob) Qi",profilePictureURL:"https://mts.intechopen.com/storage/users/300470/images/system/300470.jpg",institutionString:null,institution:{name:"Centenary Institute of Cancer Medicine and Cell Biology",institutionURL:null,country:{name:"Australia"}}}]},{id:"18",title:"Proteomics",keywords:"Mono- and Two-Dimensional Gel Electrophoresis (1-and 2-DE), Liquid Chromatography (LC), Mass Spectrometry/Tandem Mass Spectrometry (MS; MS/MS), Proteins",scope:"With the recognition that the human genome cannot provide answers to the etiology of a disorder, changes in the proteins expressed by a genome became a focus in research. Thus proteomics, an area of research that detects all protein forms expressed in an organism, including splice isoforms and post-translational modifications, is more suitable than genomics for a comprehensive understanding of the biochemical processes that govern life. The most common proteomics applications are currently in the clinical field for the identification, in a variety of biological matrices, of biomarkers for diagnosis and therapeutic intervention of disorders. From the comparison of proteomic profiles of control and disease or different physiological states, which may emerge, changes in protein expression can provide new insights into the roles played by some proteins in human pathologies. Understanding how proteins function and interact with each other is another goal of proteomics that makes this approach even more intriguing. Specialized technology and expertise are required to assess the proteome of any biological sample. Currently, proteomics relies mainly on mass spectrometry (MS) combined with electrophoretic (1 or 2-DE-MS) and/or chromatographic techniques (LC-MS/MS). MS is an excellent tool that has gained popularity in proteomics because of its ability to gather a complex body of information such as cataloging protein expression, identifying protein modification sites, and defining protein interactions. The Proteomics topic aims to attract contributions on all aspects of MS-based proteomics that, by pushing the boundaries of MS capabilities, may address biological problems that have not been resolved yet.",annualVolume:11414,isOpenForSubmission:!0,coverUrl:"https://cdn.intechopen.com/series_topics/covers/18.jpg",editor:{id:"200689",title:"Prof.",name:"Paolo",middleName:null,surname:"Iadarola",fullName:"Paolo Iadarola",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bSCl8QAG/Profile_Picture_1623568118342",institutionString:null,institution:{name:"University of Pavia",institutionURL:null,country:{name:"Italy"}}},editorTwo:{id:"201414",title:"Dr.",name:"Simona",middleName:null,surname:"Viglio",fullName:"Simona Viglio",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRKDHQA4/Profile_Picture_1630402531487",institutionString:null,institution:{name:"University of Pavia",institutionURL:null,country:{name:"Italy"}}},editorThree:null,editorialBoard:[{id:"72288",title:"Dr.",name:"Arli Aditya",middleName:null,surname:"Parikesit",fullName:"Arli Aditya Parikesit",profilePictureURL:"https://mts.intechopen.com/storage/users/72288/images/system/72288.jpg",institutionString:null,institution:{name:"Indonesia International Institute for Life Sciences",institutionURL:null,country:{name:"Indonesia"}}},{id:"40928",title:"Dr.",name:"Cesar",middleName:null,surname:"Lopez-Camarillo",fullName:"Cesar Lopez-Camarillo",profilePictureURL:"https://mts.intechopen.com/storage/users/40928/images/3884_n.png",institutionString:null,institution:{name:"Universidad Autónoma de la Ciudad de México",institutionURL:null,country:{name:"Mexico"}}},{id:"81926",title:"Dr.",name:"Shymaa",middleName:null,surname:"Enany",fullName:"Shymaa Enany",profilePictureURL:"https://mts.intechopen.com/storage/users/81926/images/system/81926.png",institutionString:"Suez Canal University",institution:{name:"Suez Canal University",institutionURL:null,country:{name:"Egypt"}}}]}]}},libraryRecommendation:{success:null,errors:{},institutions:[]},route:{name:"profile.detail",path:"/profiles/345316",hash:"",query:{},params:{id:"345316"},fullPath:"/profiles/345316",meta:{},from:{name:null,path:"/",hash:"",query:{},params:{},fullPath:"/",meta:{}}}},function(){var e;(e=document.currentScript||document.scripts[document.scripts.length-1]).parentNode.removeChild(e)}()