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1. Outline of the Wakayama arsenic murder case
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Four people were killed by arsenic poisoned curry at a summer festival on July 25, 1998, and other 63 participants were heavily injured but survived though they ate the poisoned curry. It is still not well known whether some embryos or fetuses were included within 63 or not, because the personal data is not open. The arsenic intake was authorized by the arsenic analysis of urine. One of the two curry pots was poisoned during the cooking for the preparation of the festival in a small town in Wakayama city. Wakayama is a city near the Osaka Kansai International Airport. Although the outline was reported by Kimura [1], a brief chronologically ordered outline should be described here.
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The curry was cooked in two pots in a garage of a festival organizer’s house. The curry was cooked from noon till 3 pm there, and then moved to the festival venue. During the noon and 3 pm, the curry pots were kept boiling by housewives of the organizers in turn. One of the housewives there, Mrs. H, was arrested on October 4 and prosecuted as the murder on December 29. She was sentenced to death on December 11, 2001, at the Local Court of Wakayama. Then again she was sentenced to death at Osaka Court of Appeal. Finally, May 18, 2009, the death penalty has been fixed at the Supreme Court of Japan. She has denied from the first until now, but she is now in the death row.
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The only evidence was a paper cup found near the cooking site. This paper cup might have been used to poison the curry pot bringing arsenic. Powder of about 35 mg arsenic oxide, As2O3, was left inside of the paper cup. Her husband had arsenic oxide powders as white ant pesticide, as his job was white ant exterminator. Therefore the key forensic analysis was the identification of arsenic oxide powders between her husband’s and the powder adsorbed on the inner surface of the paper cup. “High concentration arsenic” was found on one of her hairs, which was one of the several hundreds of hairs cut on December 9, 1998, by the police. These two evidences are the main reasons of her death penalty. The hair was analyzed by synchrotron radiation X-ray fluorescence (SR-XRF) and also by atomic absorption spectrometry (AAS). Several impurity elements in the arsenic oxide powders were analyzed by the SR-XRF and inductively-coupled plasma atomic emission spectrometry (ICP-AES), as was reviewed by Kawai [2]. Infrared (IR), ion chromatography/inductively-coupled plasma mass spectrometry (IC/ICP-MS), X-ray diffraction (XRD), scanning electron microscope-energy dispersive X-ray analysis (SEM–EDX), and many other chemical analysis techniques were used.
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Because the chemical poison was used by the Tokyo subway sarin attack in 1995, the Wakayama arsenic case attracted large attention by mass media, such as television, newspapers, and gossip magazines, at that time for about 1 year duration. The forensic analyses were performed mainly by the National Research Institute of Police Science, Tokyo University of Science, St. Marianna University School of Medicine, Osaka Electro-Communication University, and Hiroshima University. It was well known to the public at that time that SPring-8, one of the third generation synchrotron radiation facility, a 1.5 km circumference accelerator ring of 8 GeV, was used for the forensic analysis. The forensic analysis of SPring-8 was just 1 year after it became in use. Since 2012, Kawai, the author of the present paper, found many faults in the forensic analyses in this case, of which documents were submitted to the court from the prosecutor, and again this murder case becomes discussed in Japan.
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2. Influence on the academic researches of Wakayama arsenic murder case
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The sarin attack at Tokyo subway was just a few years before this arsenic murder case. Therefore, many academic research papers on Wakayama murder case were published, which discussed the relation of the subway sarin attack. Some examples of papers related to the Wakayama case are as follows.
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From the point of view of medical treatment at disasters, such as Matsumoto sarin attack in 1994, Tokyo subway sarin attack in 1995, Wakayama arsenic murder case in 1998, and other bombing terrorism in Japan from 1990 to 2002, were compared and discussed a future risk of terrorism and emergency management [3]. However this kind of lessons were not used at the earthquake, tsunami, and nuclear disaster at March 11, 2011, Japan. Intoxication with arsenic curry was reported in the same journal [4]. Bioterrorism threats to food were discussed [5]. Related to the subway sarin attack, importance of information sharing systems among hospitals was discussed [6], because the victims were distributed to many hospitals in Wakayama city. The patients were first treated as taking rotten food, then organophosphorus pesticide or cyanide. Therefore the information sharing was important. “FACT-Graph”, a data analysis method, was used to analyze keywords “cyanide” and “arsenic” as nodes of the graph analysis [7]. PTSD (posttraumatic stress disorder) was discussed [8]. Copycat poisoning cases, such as sodium azide (NaN3) and cyanide incidents in 1998, were discussed from the view point of chemical disaster response system [9]. A vast number of copycats appeared just after the Wakayama incidence. The importance of quality assurance against incidents of unwanted chemicals in food such as arsenic, cadmium, mercury, and lead, including Wakayama case, were systematically discussed [10]. Case seen in clinical practice at intentional acts such as nicotine, arsenic (Wakayama), rat poison, and methamidophos were discussed [11]. Economic impact of arsenic contamination in Bangladesh was studied referring to the Wakayama incidence [12].
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Concerning medical treatment, dermatology [13–16], neurology [17], and many other papers were published.
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Between the Wakayama incident on July 25, 1998, and the accusation of the suspect on December 29, 1998, many copycats were appeared mimicking poisoning [18], as mentioned above, using different chemicals, such as sodium azide, pesticides, and cyanide. At the first stage of Wakayama case, cyanide was erroneously detected, and this point was studied from the view point of chemical analysis [19–21].
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Related to analytical chemistry, ICP-AES analysis of impurity elements of arsenous oxide in order to identify the As2O3 in paper cup and that of Mrs. H’s husband were reported by researchers of National Research Institute of Police Science, Japan [22–24]. Forensic analysis using SR-XRF analysis was reported [25–29]. The importance of SR-XRF for forensic analysis was also reported in an encyclopedia [30]. LC/MS [31] and HPLC/ICP-MS [32] were reported as arsenic chemical state analysis methods related to Wakayama case. A screening method of inorganic arsenic in urine was developed [33]. A large number of other papers can be found at Google Scholar by the key words, “Wakayama arsenic”.
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3. Identification of arsenic oxide powders
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There were eight kinds of arsenic oxide evidences. Mr. M, who was a brother of Mrs. H, kept arsenic oxide powders, which were originally used as the white ant pesticide by H’s husband, long before the incidence. The evidences were as follows: (1) Paper cup, (2) M’s green 50-kg can, (3) M’s milk can, (4) M’s white can marked “Heavy”, (5) M’s brown Tupperware, (6) A milk can found at H’s old house (This house was at that time Mr. T’s house, and we call this “T’s milk can”), (7) A plastic container found at H’s kitchen, but a few particles of arsenic powders were attached on the inner surface of the container, and (8) arsenic oxide crystals found in curry pot. These are tabulated in Tables 1 and 2.
Light element concentrations of arsenic oxide powder evidences (ppm).
NA: Not available.
NA: Not available.
+: Positive.
0(+): Three tests not detected, one test detected, out of 4-time tests.
+(0): Three tests detected, one test not detected.
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The elemental concentrations analyzed using ICP-AES by the National Research Institute of Police Science are shown in Table 1. The judge wrote the death sentence by describing that one of the arsenics powders from evidences (2)–(7) was brought by H using the paper cup and put into the curry pot. However it is strange that the arsenic oxide powder concentration of the paper cup was 98.7 wt%, but evidences (4)–(6) were significantly lower than the paper cup. Finger prints were not found on the paper cup. It was known from testimony that the H’s husband bought the 50-kg green can (2) more than 10 years before, and distributed into several small cans, (3)–(6). The prosecutor guessed that H brought the arsenic powder from her old house (6) using the container (7) to her new house kitchen at house-moving. Then she brought the arsenic powder using the paper cup (1) to the curry pot.
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The National Research Institute of Police Science also analyzed five impurity elements, Se, Sn, Sb, Pb, and Bi, in (1)–(6). The chemical properties of these elements were quite similar to that of As, that is to say, Se is neighbor to As in the periodic table, Sb and Bi are in the same column, Sn and Pb are neighbors of Sb and Bi respectively, and thus they co-existed from earth crust. The concentrations of these elements were plotted as “radar chart” as shown in Figure 1, by the National Research Institute of Police Science (NRIPS). Similar radar charts of arsenic oxide powders from different industries are shown in Refs. [22] and [23]. However I found that the concentration ratios of Se/As, Sn/As, Sb/As, Pb/As, and Bi/As were multiplied by 1,000,000, then the logarithms were calculated and the radar chart was plotted by NRIPS. The pentagon radar charts of six evidences well overlap each other and looks like the arsenic oxide powders were identical as shown in Figure 1. However, both the 1,000,000 times and the logarithm are unfolded, and the radar chart is replotted including As concentration [37], then the hexagon of the paper cup (1) is significantly different from those of H’s arsenic powders (2)–(6), as is shown in Figure 2.
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Figure 1.
Radar chart of six evidences (1)–(6) taken from a document of the National Research Institute of Police Science, Japan. The document is a public document, not a copyrighted matter.
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The hexagon radar chart is interpreted as follows.\n
The regular hexagons mean the same root as M’s green 50-kg can (2).
Large regular hexagon means that the arsenic powder was more diluted. H’s husband used diluted arsenic oxide powders for white ant pesticide.
A slight distortion from the regular hexagon means the error of quantitative analysis as well as inhomogeneity of arsenic oxide in a can. Evidences (2)–(6) were sampled five times and then analyzed five times. The As concentrations of evidences (2)–(6) in Table 1 are displayed as averages ± standard deviations of five time analyses.
Significantly distorted hexagon means different roots, such as the paper cup (1). Once, one of the Bi concentration data of M’s “Heavy” (4) had a small analytical error in its concentration out of five data of NRIPS, and the hexagon was distorted. Such one-time error in five measurements can be detected by the distortion: very sensitive to different root.
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The root or origin of the paper cup (1) is significantly different from M’s green can (2). The multiplication of 1,000,000 and logarithmic calculation was in order to hide this truth.
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Figure 2.
Unfolded radar chart taken from Ueba and Kawai [37] with permission.
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The legal document of the National Research Institute of Police Science concluded that the arsenic oxide in the paper cup (1) and H’s arsenic oxide powders were less than 50% identical [38]. The small difference between paper cup (1) and H’s arsenic shown in Figure 2 could not be recognized by the XRF spectra of SPring-8. Two representative SPring-8 XRF spectra measured by the Tokyo University of Science are shown in Figures 3 and 4 [39], from which one cannot recognize the difference of the root. Though it has been revealed that spectra were measured only once for most of the evidences [40], the Tokyo University of Science concluded [39] that paper cup (1) was 100% identical to the H’s arsenic oxide powders (2)–(7) in Table 1. The details of the SR-XRF method at SPring-8 was reported in Refs. [27, 28], and it is found from these papers that the precision of the SR-XRF quantitative analysis was not high enough for the present forensic analysis, and thus the discrimination was not possible. The SR-XRF analysis conclusion was a false conclusion forced by the prosecutor [37].
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Figure 3.
SR-XRF spectrum of paper cup (1), taken from the legal document of the Tokyo University of Science [39]. The document is a public document, not a copyrighted matter.
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Figure 4.
SR-XRF spectrum of M’s green 50-kg can (2), taken from the legal document of the Tokyo University of Science [39]. The document is a public document, not a copyrighted matter.
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4. Hair analysis
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H’s hair was analyzed by St. Marianna University School of Medicine, using AAS (atomic absorption spectrometry) after NaOH digestion and hydride generation technique. They found 90 ppb As3+ in her hair. The method was the same as reported in Ref. [41]. St. Marianna University knew that As3+ was not appropriately analyzed when using the method of Ref. [41], and thus they had not analyzed As3+ from 1984 to 1997 [42]. However they analyzed As3+ in 1998 in the forensic analysis of H’s hairs and concluded that 90 ppb As3+ was exogenously attached to her hair. They used an old atomic absorption spectrometer, which was made in 1970s, i.e. too old, using paper and a pen-recorder, and measured the peak height with a rule. In early 1990s, the chemical state analysis of arsenic had been performed by an ion chromatography (IC)/ICP-MS or an HPLC/MS [43] and the analytical instruments had been computerized. The advancements of these analytical instruments were due to the zenith of the semiconductor industry [44]. Therefore St. Marianna University obtained As3+ concentration using an old non-computerized AAS machine, where chemical state of arsenic compounds changed depending on pH. Therefore the forensic analysis results on the chemical state of arsenic of H’s hairs were quite doubtful.
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Many of H’s hairs were also measured at BL-4A of KEK-PF (Photon Factory at High Energy Accelerator Research Organization, a synchrotron radiation facility in Tsukuba), and found an arsenic particle on a hair. The synchrotron radiation beam size was 4 or 1 mm width along with the hair shaft. It is still not clear how many hair shafts were measured and how many particles were found. It is said that arsenic particle was found on only one or two hair shaft(s), using 100 h of the KEK-PF beam time for hundreds of hair shafts.
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At SPring-8, the same hair was measured but arsenic signal was not detected and testified that the spectral data was deleted [44].
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Figure 5.
Photo of measuring the paper cup (1) at the beamline BL-4A of KEK-PF, taken from the legal document of the Tokyo University of Science [39]. The document is a public document, not a copyrighted matter.
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At KEK-PF, the paper cup (1) was measured, where arsenic powder was adsorbed on the surface of the cup (Figure 5). The hair shafts were measured at the same measurement chamber at the same beam time using a holder shown in Figure 6 [45]. The hair and the paper cup were handled carelessly and cross-contamination might have been happened. The detection limit of arsenic at KEK-PF was worse than 90 ppb, and thus the SR-XRF analysis results contradicted with the AAS results.
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Figure 6.
Photo of hair shaft holder used at BL-4A of KEK-PF, taken from the grant report document of the Tokyo University of Science [45]. The document is a public document, not a copyrighted matter.
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It should be pointed out that scanning electron microscope energy dispersive X-ray analysis (SEM–EDX) was not used for the hair analysis. SEM–EDX was much easier and direct observation method compared with the SR-XRF line scan. Uranium particle attached to a hair shaft was clearly observed using an SEM–EDX rather than SR-XRF, which was reported in 2015 [46], but this type of SEM–EDX analysis was possible even in 1998. It is quite strange why such a direct observation using an SEM–EDX had not been performed in 1998.
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5. Light elements analysis
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The results of light elements analysis are summarized in Table 2. This table is the results of the National Research Institute of Police Science. M’s green can (2) was pure arsenic oxide imported from China. M’s milk can (3) was directly taken from the green can (2). If Table 2 is compared with Table 1, the concentrations of the impurity light elements are mostly inversely related to the arsenic concentration. That is to say, when As wt% was less, then Al and/or Ca concentrations were higher, for e.g., M’s “Heavy” (4), M’s brown tupper (5), and T’s milk can (6). However, paper cup (1) was different. Sodium and iron concentrations were higher, but arsenic concentration was also high. If saline water like the sea water about one litter was poured into another green 50-kg can, and then dried, sodium concentration could be explained, but the sea water should take the mass ratio Na:Mg:Ca=100:12:4, which was significantly different from the ratio in Table 2. Based on the discussion at Section 3 and the present section, the paper cup arsenic oxide powder (1) was taken out from another green can imported from the same industry, but this green can was once exposed to saline water containing Na, Mg, and Ca, when mining, smelted, shipping, or in use.
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It is known that arsenic green 50-kg cans were imported from China twice in a year for total 10 years. The M’s green can was one of the 60 cans imported at the same time by a ship, known by the shipping mark on the can. At the top, 10 or 15 cans were sold in Wakayama city in a month, and consequently at least more than one hundred cans were sold in Wakayama city before the arsenic curry incidence.
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Starch was found for several arsenic oxide powder evidences using infrared (IR) analyses twice and iodine-starch tests twice. M’s brown tupper (5) and T’s milk can (6) results were contradicted as shown in Table 2. The paper cup (1) did not contain starch, and if the sentence was correct, starch powder mixed in the arsenic disappeared when taken by the paper cup.
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Barium is not a light element but was found in several arsenic oxide evidences. Barium was an impurity element in SiO2 for M’s brown tupper (5), because barium was not water soluble. Barium was an impurity element of Ca for T’s milk can (6), because it was water soluble; calcium was due to the cement. However, the barium in paper cup (1) has not been analyzed whether water soluble or not. Based on these fact, Osaka Electro-Communication University and Hiroshima University, who performed forensic analysis according to the order of judge in 2001, concluded that paper cup arsenic oxide (1) was identical to either M’s brown tupper (5) or T’s milk can with the probability of 80% [47]. But this was wrong, because the concentration of arsenic was higher for paper cup than those of (5) or (6); also because of the discussion related to the hexagon radar chart in Section 3.
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6. Summary
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I have published comments on the forensic analyses on Wakayama arsenic poisoned curry [2, 34, 35, 38, 40, 42, 44, 47–55], and revealed many false and truth-hiding reports step by step. Nakai of the Tokyo University of Science published papers in order to refute the above comments, but the refutations were not successful, and recently he has kept silence. The prosecutor sought some authoritative professors who could write documents against Kawai, but failed to find. The earlier discussion in the literature was cited by Chemistry Views [56], Spectroscopy Now [57], and Russian review paper [58].
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The false forensic analyses were documents of National Research Institute of Police Science, of the Tokyo University of Science, of both Osaka Electro-Communication and Hiroshima Universities, and of the hair analysis of St. Marianna University School of Medicine. These four forensic reports had main role in the death penalty of Mrs. H. Forensic analysis reports of other cases have been checked by me, and it was found that many of them were also false [44, 55]. Neufeld and Scheck [59] launched “Innocence Project” early 1990’s and many death row prisoners were released from jail. The innocence project in US was based on DNA analysis, but many other forensic analysis methods have been improved [60] due to the innocence project. Compared Japanese situation with US, the quality of forensic analysis is poor as discussed above. The National Research Institute for Police Science is not an independent research institute and they use forensic analysis in order to arrest a suspected person, but not for the proof of innocence. This is a big problem for the administration of justice in Japan.
\n
False of four main forensic reports in Wakayama arsenic case means that all the important forensic reports submitted from prosecutor are false in Japan.
\n
\n\n',keywords:"X-ray fluorescence (XRF), inductively coupled plasma (ICP), synchrotron radiation (SR), atomic absorption spectrometry (AAS), Wakayama arsenic poisoning case",chapterPDFUrl:"https://cdn.intechopen.com/pdfs/50757.pdf",chapterXML:"https://mts.intechopen.com/source/xml/50757.xml",downloadPdfUrl:"/chapter/pdf-download/50757",previewPdfUrl:"/chapter/pdf-preview/50757",totalDownloads:2523,totalViews:1417,totalCrossrefCites:0,totalDimensionsCites:0,totalAltmetricsMentions:0,impactScore:0,impactScorePercentile:9,impactScoreQuartile:1,hasAltmetrics:0,dateSubmitted:"October 31st 2015",dateReviewed:"April 8th 2016",datePrePublished:null,datePublished:"September 7th 2016",dateFinished:"May 17th 2016",readingETA:"0",abstract:"This is a review paper of forensic analysis of a murder case of Wakayama arsenic poisoning incident. The influence of this case on scientific research was not small in such a way that papers related to PTSD, disaster medical, copycats, chemical analysis, unwanted chemicals in food, terrorism, and so on were published. The forensic analyses on Wakayama arsenic poisoning incidence have characteristic that SPring-8, a largest synchrotron radiation facility, was used, as well as many other analytical techniques, but now most of the forensic analyses submitted from the prosecutor have been revealed to be fabrication, hiding the truth by logarithmic calculations, and therefore not scientific. Most of the testimonies at the court by the analysts were also lies. Examples of such false analyses are explained.",reviewType:"peer-reviewed",bibtexUrl:"/chapter/bibtex/50757",risUrl:"/chapter/ris/50757",book:{id:"5259",slug:"forensic-analysis-from-death-to-justice"},signatures:"Jun Kawai",authors:[{id:"180878",title:"Prof.",name:"Jun",middleName:null,surname:"Kawai",fullName:"Jun Kawai",slug:"jun-kawai",email:"kawai.jun.3x@kyoto-u.ac.jp",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:{name:"Kyoto University",institutionURL:null,country:{name:"Japan"}}}],sections:[{id:"sec_1",title:"1. Outline of the Wakayama arsenic murder case",level:"1"},{id:"sec_2",title:"2. Influence on the academic researches of Wakayama arsenic murder case",level:"1"},{id:"sec_3",title:"3. Identification of arsenic oxide powders",level:"1"},{id:"sec_4",title:"4. Hair analysis",level:"1"},{id:"sec_5",title:"5. Light elements analysis",level:"1"},{id:"sec_6",title:"6. Summary",level:"1"}],chapterReferences:[{id:"B1",body:'\nY. 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Chichester: Wiley (2004) pp. 553–567.\n'},{id:"B30",body:'\nR. Cesareo: X-ray fluorescence spectrometry. In: “Ullmann’s encyclopedia of industrial chemistry”, Ed. B. Elvers. Weinheim: Wiley-VCH (2010, 2012) Vol.39, pp.595–631.\n'},{id:"B31",body:'\nK. Kudo, H. Tsuchihashi, N. Ikeda: Meeting challenges in forensic toxicology in Japan by liquid chromatography/mass spectrometry. Analytica Chimica Acta. 492:83–104 (2003).\n'},{id:"B32",body:'\nB. K. Mandal, Y. Ogura, K. T. Suzuki: Speciation of arsenic in human nail and hair from arsenic-affected area by HPLC-inductively coupled argon plasma mass spectrometry. Toxicology and Applied Phermacology. 189:73–83 (2003).\n'},{id:"B33",body:'\nL. Hua, M. Nishida, A. Fujiwara, M. Yashiki, M. Nagao, A. Namera: Preliminary screening method for the determination of inorganic arsenic in urine. Legal Medicine. 11:80–82 (2009).\n'},{id:"B34",body:'\nJ. Kawai: Problems of the forensic analysis in Wakayama arsenic case. 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In: Report on the Grant-in-Aid for Scientific Research 1999 from Ministry of Health and Welfare, 199900701A0002 (2000). http://mhlw-grants.niph.go.jp/niph/search/NIDD02.do?resrchNum=199900701A\n'},{id:"B46",body:'\nA. Israelsson, M. Eriksson, H. B. L. Pettersson: On the distribution of uranium in hair: Non-destructive analysis using synchrotron radiation induced X-ray fluorescence microprobe techniques. Spectrochimica Acta Part B. 108:28–34 (2015).\n'},{id:"B47",body:'\nJ. Kawai: How to find false statements in expert document #4: identical, same kind, or similar. Quarterly Keiji-Bengono. 87(Summer), (2016) in press.\n'},{id:"B48",body:'\nJ. Kawai: Revisit of forensic analysis of arsenic poisoning case 1998. Advances in X-ray Analysis. 57:177–184 (2014).\n'},{id:"B49",body:'\nJ. Kawai: Reviews on forensic analysis of Wakayama arsenic case: X-ray fluorescence analysis—submitted to court. Advances in X-ray Chemical Analysis Japan. 43:49–87 (2012).\n'},{id:"B50",body:'\nJ. Kawai: Light element analysis of Wakayama arsenic case. Advances in X-ray Chemical Analysis Japan. 44:165–184 (2013).\n'},{id:"B51",body:'\nA. T. Tu, J. Kawai, K. Oda, Ed. S. Ishizuka: Criminal case and forensic analysis. Ryukoku Law Review. 46(4):1141–1206 (2014).\n'},{id:"B52",body:'\nJ. Kawai: Role of desktop X-ray fluorescence analysis of Wakayama. Advances in X-ray Chemical Analysis Japan. 45:71–85 (2014).\n'},{id:"B53",body:'\nA. T. Tu, J. Kawai: Role of infrared absorption spectroscopy in the forensic analysis of Wakayama curry arsenic poisoning case. Advances in X-ray Chemical Analysis Japan. 45:87–98 (2014).\n'},{id:"B54",body:'\nJ. Kawai: Was forensic analysis of Wakayama poisoned curry case reliable enough? Chemistry Today. 507(June):42–46 (2013).\n'},{id:"B55",body:'\nJ. Kawai: Chemical analysis and forensic analysis—Shiratori case, nylon rope case, pig iron case, Wakayama curry case. 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Expert Evidence Reporter. 1(4):93–97 (1989).\n'},{id:"B60",body:'\nCommittee on Identifying the Needs of the Forensic Sciences Community, National Research Council: Strengthening Forensic Science in the United States: A Path Forward. The National Academies Press, Document No. 228091 (2009). http://www.nap.edu/catalog/12589.html, https://www.ncjrs.gov/pdffiles1/nij/grants/228091.pdf\n'}],footnotes:[],contributors:[{corresp:"yes",contributorFullName:"Jun Kawai",address:"kawai.jun.3x@kyoto-u.ac.jp",affiliation:'
Department of Materials Science and Engineering, Kyoto University, Sakyo-ku, Kyoto, Japan
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1. Introduction
1.1 Smallholders’ agricultural production and productivity in Africa
In Africa, smallholder agriculture is predominant and agricultural growth and poverty reduction are subjects closely associated with growth in smallholder agriculture for some time to come. An estimated 41 million smallholders [1] are the major source of food for nearly all rural and most urban dwellers in Africa. In Sub-Saharan Africa (SSA), most smallholders own less than two hectares holding of cultivable land and are challenged by the low productivity and production constraints in the middle of the unprecedented rising need for more food, feed, and raw material for industry. The SSA region alone has a quarter of the world’s arable land endowment but produces only 10% of world agricultural output [2]. Unlike smallholders in Asia who dominantly grow few crops such as rice and wheat, African farmers experience diverse farming systems and grow very diverse crops that include maize (Zea mays), sorghum (Sorghum sp) millet (Penisetum sp), wheat (Triticum aestivum), and rice (Oryza sativa); pulses such as soybean (Glycine max), cowpea (Vigna unguiculata), beans (Phaseolus sp.), groundnut (Arachis hypogaea), and other crops such as cassava (Matnihot esculentus), sweet potato (Ipomoea batatas), potato, (Solanum tuberosum), yam (Dioscorea sp), banana (Musa sp), cotton (Gossypum sp), and sugarcane (Saccahrum officinarum) (Table 1) [3].
Sorghum, pearl millet, pulses. Sesame and livestock
Sparse (Arid)
17
1
Irrigated maize, vegetables, date palms, cattle
Table 1.
Major farming systems of sub-Saharan Africa.
Source: FAO and World Bank, Rome and Washington DC 2006. (Adapted to show more crop-based farming system).
Crop productivity in Africa specifically in the SSA region is below the world average (Figure 1) and the region constitutes the highest number of food-insecure population (35.5% of its population) of whom 21.3% are severely insecure [4] rendering the region increasingly dependent on imported food. Due to this and other factors about 39 countries of the SSA account for the largest number of food-insecure people: 424.5 million (40.5% of the region’s population) in the year 2020 [5]. It can also be seen that during the period 1961–2018, cereal yield in Africa has grown only one fold compared to a 2.5 fold increase in Asia, which had only 26.3% area increase compared to Africa with 1.2 fold increase (Figure 1). Therefore, whatever growth there has been in cereal production in Africa, it was largely due to land expansion in contrast to Asia. Food insecurity is forecasted to worsen due to climate change impacts and recurrent drought unless proper and quick measures are implemented [6]. The region will have a shortfall of nearly 90 million metric tons of cereals by the year 2025 if current agricultural practices remain unchanged. Productivity trends do not promise a better future for cereals and roots and tuber crops as can be seen from cereal performance during the period 1961–2018 average yield based on FAOSTAT data 2020 (Figure 2).
Figure 1.
Change (percent increase) of cereal yield and land used for cereal production. (Data source: Computed from Food and Agriculture Organization (FAO) of the United Nations. 2019 Report).
Figure 2.
Yield (t/ha) trends of cereal production in different regions of the world. (Data Source: Food and Agriculture Organization of the United Nations. 2019 Report).
However, more factors are known to involve in constraining smallholder farmers’ crops production and cause yield gaps. Low crop productivity is often related to biotic stresses such as those caused by insect pests, diseases, and weeds as well as the inherent low-yielding potential of varieties, and abiotic stresses caused by soil-related and climatic problems such as moisture stress and drought. The latter is a pronounced problem of vast marginal and drier agriculture areas of SSA. Crops grown in such marginal environments are exposed to frequent severe growing conditions. Each factor is responsible for substantial yield losses annually by smallholder farming. Furthermore, yield gains associated with high-yielding varieties if found much lower in SSA partly due to inadequate inputs, poor infrastructure, and market outlet including weak extension services. Thus, poor availability of improved technology packages (improved seeds, irrigation, fertilizers, and pesticides) makes it hard for millions of smallholder farmers to produce surplus and escape the subsistence type of life.
Successful mitigation of these biotic and abiotic constraints and institutional limitations affecting agricultural growth is a task that not only requires political will and sustained commitment by country governments in Africa, but also a stronger global collaborative effort to realize enhanced applications of modern technologies to complement and transform the conventional interventions efforts underway. Increased investments in agricultural R&D and fast-tracking the use of innovative technologies such as conventional as well as modern biotechnology and proven useful readily available biotechnology products is extremely needed to solve smallholder farmers’ crop productivity problems. As such agricultural biotechnology offers enormous opportunities through innovative ideas, techniques, and processes to drive innovative solutions highly relevant for the needs of smallholder farmers in Africa [7]. Medium to long-term benefits of using advanced techniques of biotechnology that include tissue culture, micropropagation, gene, and marker discovery, genomics, genetic engineering, genome-editing, bioinformatics, and others through enhancing crop breeding including indigenous crop species cannot be overemphasized [8]. This chapter focuses on the deployment of modern biotechnology such as genetic engineering tools and products as well as challenges facing adopting countries in developing Africa. It also presents case studies of agricultural biotechnology uses and progresses in six countries in SSA focusing on the use of safe biotechnology crops to solve key biotic and abiotic constraints faced by smallholder farmers in the respective countries.
1.2 Promises of biotechnology to smallholder farmers
The rapid advancements in the field of biotechnology offer promising alternatives to the approaches of crop improvement. Biotechnology complements and makes the conventional breeding efforts in crops efficient through precise identification and introgression of genes in a much shorter time period. The integration and development of biotechnology research in national research programs is now a prerequisite for current and most of the future science-based sustainable genetic improvement of crops for various purposes including, food and nutritional security, improving post-harvest and industrial qualities of cereals, horticultural and forage crops.
It is clear that smallholder farmers in African countries are currently not benefiting enough from modern biotechnology, which can be applied to transform their crop production and productivity and bring about livelihood improvements. Most national research programs in Africa have not yet acquired research and regulatory capacity and skills to integrate advanced science and cutting-edge technologies in their research portfolio to solve farmers’ production problems. Although progress is registered in biotechnology capacity building in some countries, it is far from adequate. Governments’ investment in agricultural research and development is generally low [9]. Crop productivity problems under smallholder farmers’ conditions are often caused by low-level use of improved technologies and damage to crops caused by biotic and abiotic stresses as described earlier. The biotic and abiotic stresses challenging crop productivity are being tackled by biotechnology globally and several crop varieties with novel traits have been successfully developed and commercialized in more than 25 countries around the world to solve particular production problems of farmers.
1.3 Crop improvement programs in Africa
Food security and prosperity in Africa depend much on its agricultural performance. Ensuring sustainable development in agriculture is critically dependent on a sustainable technology supply and uptake. Despite the strong need for robust agricultural research, capable of tackling production constraints under challenging agricultural environments, African countries have not shown much progress in their national research capabilities to respond to food security issues and meet the overarching national strategic goals for sustainable development [9]. Strategic measures pursued to realize latecomer advantages in using modern biotechnology to enhance crop improvement and exploiting existing commercialized novel biotechnology products proven safe and impactful, is weak.
Reports show declining government R&D spending in the agricultural sector recently from 0.59% in 2000 to 0.39% in 2016 in the SSA [10]. Thirty-three of the 44 SSA countries have less than the minimum investment target of 1% AgGDP (Figure 3) recommended by the African Union and United Nations [11]. Thus, most national programs in Africa were not able to maintain up-to-date capacity in trained human resources and facilities to translate scientific research into useful products impacting agricultural growth. Conventional crop improvement programs are increasingly requiring support from biotechnology to effectively respond to changing market demands. Therefore, African government should play a key role to strengthen national programs and maintain strong regional and global collaborative partnerships and expedite knowledge and technology transfer. Allowing more regional integration can help to ensure smoother collaboration, transfer of suitable technologies, data and information, and allows improved access to products at an affordable price and quality [12].
Figure 3.
Some SSA countries and their R&D investment share as a percent of AgGDP (except the top ranking the last three countries, all the others are selected only for representation of the rest). Source: Data sourced from ASTI [10].
Most African countries have not created the necessary incentives for high-end modern biotechnologies to get well integrated in the research and development profile of national programs and create opportunities for new products to get to market. Instead, they depend on other countries that have decided to invest and strengthen their R&D. They are not taking advantage of this to enable national programs to expedite adoption and use of better and diverse technologies through quick testing and approval processes. Biotech products are rapidly expanding to include not only farmers’ interest but getting more diversified targeting the interest of industry and consumers [13]. Therefore, a further declining trend of investment in agricultural R&D over the past 15–20 years in the developing countries with few countries in exception is alarming [14]. In countries with advanced economies where public financial outlay for R&D has lagged, the private sector has been investing heavily in genomic sciences and techniques that enable faster and more efficient delivery of improved crops to farmers, the value chain, and consumers, targeting business opportunities and crops with the greatest returns to investment [7]. However, many ‘orphan’ or underutilized indigenous crops in developing countries have been forgotten and their diversity is threatened [7]. It is highly challenging to rectify this imbalance between public and private research investment and ensure that crops including indigenous species are improved and conserved thus equally benefiting from modern biotechnology.
Against all odds and considerable skepticism in African countries even after three decades of the phenomenal growth of modern biotechnology and wider adoption of safe biotechnology crops globally, some countries have moved forward and strengthened capacity in biotechnology and related fields of biosafety, food safety, and intellectual property (IP) management to reap the benefits of integrating the advanced sciences. The recent progress in approvals of several biotechnology crops in Africa can reverse the delay in the near future [15, 16, 17, 18].
2. Role of agricultural biotechnology: narrowing yield gaps
Rapid advancement is made in the field of biotechnology since the discovery of DNA and during subsequent advancements in molecular techniques and other “omics” technologies. This has ushered agriculture into a new era of technological frontiers to tap the latent potential of its biological resources in an unprecedented way, showing a new horizon of opportunities emerge to develop and modernize agriculture. Today, modern agricultural biotechnology encompasses a range of technologies including molecular breeding, fingerprinting, genomics, proteomics, genetic engineering, genome-editing, tissue culture and micropropagation techniques, and other advanced applications. This has empowered scientists, provided unlimited potential, to develop new strategies to harness genetic potentials for solving current and emerging crop production challenges. Therefore, biotechnology has provided a unique capacity to successfully fighting back the continuing battle against diseases, pests, and environmental stresses that are global threats to the survival of mankind. Genetic engineering, a part of modern biotechnology, involves the manipulation of the gene(s) of crop species by introducing, eliminating, or editing specific gene(s) through modern molecular techniques.
During the 1970s and 1980s, the public sector began supporting biotech research with lots of anticipations to advance the use of genetic engineering in agriculture soon to be taken over by the private sector. The first genetically modified (GM) plants were successfully developed as early as 1983 using antibiotic-resistant tobacco and petunia. In 1990, China started to commercialize GM tobacco for virus resistance followed by the Flavr Savr tomato in the United States. By 1995 and 1996, several transgenic crops were approved for large-scale use. Since the first commercial delivery in 1996, millions of smallholder farmers around the world have become beneficiaries of the multiple benefits from growing GM crops [19, 20].
Farmers are primary beneficiaries of the improved production and associated positive environmental, socio-economic, health impacts [21]. The rapid adoption and expansion of biotech crops reflect the substantial multiple benefits realized by farmers in industrial and developing countries. To date, of interest to farmers are several GM crops with enhanced input traits, such as disease (viral, fungal, bacterial) and insect resistance, herbicide tolerance, and resistance to environmental stresses such as drought, improved processing quality, improved product shelf life, and nutrient-enhanced crops available for commercial production.
Recent data [19] shows global acreage of only four biotech crops, corn, soybean, cotton, and canola has reached 190.4 million hectares in 2019 from 1.7 million hectares in 1996, which is on average 7.9 million hectares growth per year impacting crop production and productivity [22]. In recent years, the novel technique of genome-editing (GE) has been developed for targeted genome modification in plants with a high potential of increasing genetic diversity or correcting genetic defects. The simplicity and high efficiency of these tools have made it optimal for precise genome editing, heralding a new frontier in the—“Gene-revolution”—and in the development of modern biotechnology.
GM technology has been targeting some of the yield constraints and successful technologies have been commercialized in Africa for different crops such as insect resistance (maize, cotton, soybean, brinjal, cowpea), disease resistance (cassava, potato, sweet potato), better nutrition and quality (rice, potato, sorghum, banana). Some of these technologies are now successfully tested or grown in some countries of Africa (Table 2). Globally, by the end of 2019, a total of 71 countries (excluding EU countries) [19] issued regulatory approvals for GM crops, of these 11 were African countries. Total approval granted between 1992 and 2018 has reached 4349 from 70 countries (28 countries from EU) for food (2063), feed (1461), and environmental release or commercial cultivation (825) of GM plants [23]. In 2020 alone, 43 approvals were recorded for GM crops globally, involving 33 varieties from 12 countries, and eight of them are new varieties [22]. In 2019, four countries in Africa have given commercially approved for GM crops namely Ethiopia, (Bt cotton), Malawi (Bt cotton), Kenya (Bt cotton), and Nigeria (PBR cowpea) for the first time. Nigeria had additional approval for TELA maize in October 2021 and Kenya approved GM Cassava in June 2021. The TELA maize is built on the progress made from a decade of excellent breeding work under the WEMA project and working toward introducing the Bt- gene to WEMA, water-efficient varieties for drought tolerance [15, 16].
Country
GE crops researched, under testing, under approval process and/or approved
Commercialization (year)
Burkina Faso
Cowpea (insect resistance to Maruca pest); Bt cotton resistance to insect pest Bollworm) Rice (Resistance to Xanthomonas oryza)
Cotton (2008) suspended from production in 2016*
Cameroon
Cotton (stacked insect resistance and herbicide tolerance)
Despite several crops under testing for a long period, only a few have been commercialized in Africa (Table 2) [24]. In the SSA, South Africa has taken the lead with an estimated 2.7 million hectares covered with GM crops. It grows three commodities, namely cotton (100% cover), maize (85%), and soybeans (95%) of the total acreage [25]. Nigeria follows with three approvals (Bt cotton, PBR Cowpea, and TELA Maize) since 2018 [17], whereas Sudan stands second in acreage (about 192,000 hectares) from Bt cotton production.
Yield and quality improvements and associated economic benefits of growing GM crops have been the driving factors for biotech crops’ rapid global expansion. A study conducted on GM crops and conventional hybrid (CH) maize yield differences across 106 locations and over 28 years in South Africa has shown a mean yield increase for GM over CH maize of more than 0.42 MT per hectare in addition to reducing yield risks [26]. Others reported [27] that GM technology adoption has reduced chemical pesticide use on average by 37%, increased crop yields by 22%, and increased farmer profits by 68%. According to the report, yield gains and pesticide reductions are larger for insect-resistant crops than for herbicide-tolerant crops, and yield and profit gains are higher in developing than in developed countries.
3. Farmers access to new agricultural technologies
Since the first field trial of a GM product back in 1987, the world has seen massive progress in the adoption of biotechnology crops and products and an increasing number of laboratory and field trials for a variety of novel GM products. Of the total global acreage (190 million hectares) of GM crops in 2019, the share of African countries is close to 3.0 million hectares only with South Africa taking the lead with 2.7 million hectares for HR-soybeans, IR/DT- maize and Bt cotton, followed by Sudan for 192,000 hectares of Bt- cotton [21, 28]. Currently, however, 13 biotech crops containing 13 traits in 13 countries are under different stages of research and evaluation in Africa [21]. Crops such as cotton, maize, cowpea, rice, sorghum, potato, sweet potato, cassava, banana, and sugarcane are either at the stage of Confined Field Trials (CFT) or commercial production status [29]. Since 2018, four countries have entered commercial production for the first time in Africa namely, Nigeria (Bt cotton and PBR cowpea in 2018 and TELA maize in 2021), Kenya (Bt cotton in 2020 and virus resistant cassava in 2021), Ethiopia (Bt cotton in 2018), and Malawi (Bt cotton in 2018), after approval for the respective GM crops [19, 20]. Nigeria has made a move to become the first among African nations followed by Kenya that approved commercial use of GM food crops cowpea and maize.
Given global advancement in the use of GM crops, progress in Africa has been slower than expected [30, 31]. After three decades of global experience on the safety of GM crops and impressive impacts on the livelihood of millions of farmers, many countries still are postponing approvals of GM crops. Numerous health and environmental safety research reports have sufficiently confirmed the safety and desirable impacts of GM crops and their derived products [30, 31, 32, 33, 34]. Such scientific evidence have not challenged enough the lingering public perception and controversies around the risks of GM crops [35]. Instead, the overwhelming challenges faced by farmers make it difficult to believe these technologies can positively affect the situation of smallholder farmers [31]. However, scientists believe genetic engineering and genome-editing technologies will continue to impact the global economy with new momentum for more innovative technologies. Countries such as Ghana, Tanzania, Ethiopia, Mozambique, Uganda, and Malawi are in process of working on clarifying the biosafety context and developing a guideline for promoting genome-editing technologies in crop improvement [36].
3.1 Factors shaping access and availability of biotech products for smallholder farmers
The commercialization of already approved products is challenged by a wave of issues along the product commercialization chain. The national research capacity has been very critical to respond to farmers’ needs for new technologies through creating awareness to the public, advising policymakers, testing of technologies, approvals, and helping access to proven technologies by farmers. In the same way robust regulatory system is needed to respond to applications based on scientific and empirical evidence. Often this has been a challenge in most countries since sufficient safety data generated can only be accepted and reviewed again by the regulatory agency of adopting country. Private and public sector developers apply step-wise review and decision processes to critically monitor the development of new products and to ensure that only good events are commercialized. Therefore, the intellectual property, product stewardship, and commercialization strategy become key parts of the product life cycle.
The Excellence Through Stewardship (ETS) [37], a global industry coordinated organization, identifies the key steps in the biotechnology product life cycle which includes the following: (i) research and discovery; (ii) product development; (iii) seed or plant production; (iv) marketing and distribution; (v) crop production; (vi) crop utilization; and (vii) product discontinuation (Figure 4). Product Stewardship and commercialization are key cross-cutting components along the product life cycle for the industry to remain innovative and viable. Successful commercialization of a GM crops, therefore, requires a well-planned strategy with sufficient information and expertise in a wide range of professions spanning from research and discovery to market and consumer interest.
Figure 4.
Biotechnology product life cycle (Excellence Through Stewardship, 2018). Source: Excellence through Stewardship (2018).
In other words, success in commercialization also depends on downstream activities: functional seed systems and extension systems, strong technology demonstration, presence of reliable financial and marketing services, and the like. These are often weak in developing countries including most parts of Africa. The blame on lack of political will, safety concern, or public acceptance for the delay in the adoption of deregulated products is often misleading. A recent assessment of stakeholders view on commercialization barriers of released biotech products shows socio-economic constraints, high cost of seed, weak certification of seed, weak private sector involvement, inadequate awareness of the technology, and best practices to be important [18, 24, 38, 39]. Thus, potentially a stronger public-private partnership in research, product development, and product commercialization in developing countries holds the key.
3.2 Challenges of scaling-up and utilization of biotech crops
Rigorous risk assessment studies take years to complete only to satisfy the benefit of the doubt. In Africa, many consider GM crops are intended for use in industrialized countries and are hence inappropriate for agriculture in Africa. There is a poor understanding of the use and potential impact of the technologies on improving productivity. In some countries, GM crops are considered a threat to biodiversity due to fear of replacing local or conventional varieties and indigenous crop species and thereby making farmers dependent on private seed companies. Limited research, regulatory and monitoring capacities, and anticipated loss of export markets with trade-sensitive countries also add up to the challenges against wider commercialization of the biotech crops [38]. In countries that have overcome hurdles of the regulatory system, rolling of GM crop commercialization and access by growers depend much on what happens downstream the pathway beyond product development, regulatory approval, and registration.
3.3 Enhancing regulatory decisions for improved access
Delayed decisions from regulatory agencies have a large, negative impact on the commercialization of new GM crop varieties around the world, but also in Africa [28]. While some delays can be sustained by some private sector developers, public sector developers are reliant on funding cycles and their projects are more quickly discontinued by indecision at regulatory agencies [40]. Regulators can strengthen decision-making by first reviewing the safety of new GM products and then linking the decision to national policy goals such as food security, sustainability, and the economic benefits to local farmers [41]. Linking regulatory decisions on GM plants to national policy goals, such as achieving the UN Sustainable Development Goals (SDGs), will help to clarify which products benefit the community, the environment, and bring about economic growth [18].
3.4 Seed access
After going through national performance and verifications studies to satisfy national variety release and registration requirements [29], the product deployment is carried out by the technology owner, mostly a private company, through technology demonstration and demand-based seed supply. In this process, roles and stakeholder institutions change where the private sector, seed system, extension system, and other regulatory and financial institutions take over and function in subsequent steps. These transitions are not always clearly defined where the public sector is a major supplier of improved seed or where the seed sector is predominantly informal as in most African countries. Therefore, the commercialization of GM crops is overburdened with multiple issues of promoting new and approved products.
Weak seed systems and weak credit systems limit product access by farmers. A recent study on Bt-cotton hybrid seed access by farmers indicates that weak coordination among various stakeholders along the seed value chain is shown to exacerbate the problem of sustainable supply and wider utilization of the approved GM products [38, 39]. Lack of awareness of role players, inadequate demonstration of new technology to farmers as well as poor handling of the new technology by farmers, and poor extension schemes also contribute to the poor commercialization observed. Socio-economic constraints such as the high cost of hybrid seed, weak certification of seed, and inadequate awareness of technology and best practices (seed handling, agronomy, etc) can become important factors that can slow or block progress in some countries [38]. This also requires a stronger public-private partnership to advance the integration of modern biotechnology in the national R&D system.
4. Country case studies
4.1 Burkina Faso
4.1.1 Country progress
Burkina Faso has signed the Cartagena Protocol on Biosafety in 2003. It has an active and functional regulatory system hosted by the National Biosafety Agency (NBA) (Agence Nationale de Biosécurité, ANB) currently exercising Biosafety laws, regulations, policies, and guidelines in the country. In addition, at a regional level, the Economic Community of West African States (ECOWAS) has put regional framework and rules on biosafety. The NBA is hosted by the Minister of Higher Education but has consultative bodies such us National Scientific committee of Biosafety (comité scientifique national de Biosécurité = CSNB), Scientific and Technique Council, National observatory of Biosafety regrouping members from various ministries and non-governmental organizations.
4.1.2 Product development
The NBA has approved different research activities on GM crops. From 2006 to 2015 about 32 permits for different GM cotton activities related to BollgardII, RRF (herbicide tolerance), and the stack of both were made for import, laboratory studies, CFT, commercialization, and seed production activities. From 2010 to 2021, there were six permits given for Maruca Pod Borer resistant GM cowpea using Cry1Ab or Cry2Ab genes for greenhouse and CFT. Other GM crop permits provided include for CFT on Bt Maize for insect resistance; greenhouse trial for vitamin and zinc-rich biofortified sorghum; greenhouse trials for leaf blight resistance in rice.
Only the Bt cotton Burkina Faso had reached the stage of commercialization and utilization. However, the Bt cotton cultivation was discontinued in 2016 due to cotton fiber length issues associated with the marketing of Bt cotton. Currently, most of the research activities are carried out in the greenhouses, cages, and CFTs. In Burkina Faso, stakeholders support the use of GMO as a solution to food security and for human disease control such as Malaria. The ANB has been undertaking sensitization of various public entities and various stakeholders since 2009 on biosafety actions as described by the national legislation and the Cartagena Protocol.
4.2 Ethiopia
4.2.1 Country progress
Ethiopia signed the Convention on Biological Diversity (CBD) in 1993, Cartagena protocol in 2000 which was approved by Parliament in 2003. The country adopted a tighter regulatory framework based on the Precautionary Principle (equivalent to “No GMO”) ratified in 2009. The Biosafety bill was debated amended in 2016, known as ‘A Proclamation to Amend the Biosafety Proclamation 2009’. In 2017, the National Biosafety Advisory Committee was adopted and in 2018 the country issues its Biosafety Guidelines. The amended law permitted scientists and institutions to do research and education pertaining GMOs. This allowed to establish legal and regulatory systems and build technical capacity to support and manage GMO issues and approved after CFT of three Bt cotton varieties in 2016 under the procedure of “Special permit”, a provision in the Biosafety Law for research purposes. This was followed by 2 years of NPT across seven sites until 2018. The country approved two Bt cotton hybrids, JKCH-1050 and JKCH-1947 originally obtained from JK Agri Genetics Ltd., India for environmental release and variety registration. The accelerated commercial release demonstrated Ethiopia’s government commitment to support the cotton development to satisfy booming textile industries [29].
4.2.2 Product development
Ethiopia considered biotechnology as one of the priority areas in its National Science and Technology Policy formulated in 1993 [42]. Due to interest to tighten the non-GMO stand, the prohibitive regulatory system delayed its overall engagement in modern biotechnology, postponed the use of available products, and hampered the development of the local capacity building. After approval of two Bt cotton Bollgard I type varieties in 2018, demand for Bt cotton seed for 2021/22 estimated at 3250 kg was requested for 1300 hectares. Some level of cross-border Bt cotton seed also takes place with Sudan and around 3055 hectares around border areas are already covered with such imported Bt cotton seed.
In 2008, the Biosafety Authority and the NBAC granted a “Special Permit” approval for CFT of drought-tolerant (WEMA) and insect resistant (TELA) maize for testing from 2018 to 2023. The isogenic conventional lines were evaluated for 2years in different locations before the CFT. The two-year CFT was started in 2019 under a controlled drip irrigation system for drought-tolerant trait evaluation and has shown very promising results. The stacked maize environmental release for both insect resistance and drought tolerance is awaiting approval using existing provisions.
In 2013, Ethiopia deployed GM technology for its indigenous Enset crop (also called “false banana”) improvement in collaboration with the International Institute of Tropical Agriculture (IITA) for developing varieties with resistance to the deadly bacterial wilt disease caused by Xanthomonas campestris [43]. The collaborative research work had begun at BecA, Nairobi at IITA laboratory and later moved to Holetta Agricultural Biotechnology Research Center (NABRC) in Ethiopia in 2018 after approval was obtained for contained use (Contained Lab Permit). Approval for testing transgenic Enset under CFT is underway. Further to its endeavor in GM technologies, Ethiopia will soon engage in testing Late Blight Resistant (LBR) resistant cisgenic potatoes. Application submitted for CFT is awaiting approval.
4.2.3 Farmers access to new agricultural technologies
Approved Bt cotton hybrid seed demand is increasing but the hybrid Bt cotton seeds are not locally available and need to be imported from the technology supplier. But due to the decline in exports during the COVID-19 Pandemic, the Bt cotton seed supply system has suffered from foreign exchange restrictions to purchase seeds. The absence of local seed companies investing in Bt cotton seed has been one of the key challenges facing Bt cotton commercialization in Ethiopia.
Stakeholders across the cottonseed system must assess the most feasible pathway to ensure easy access to quality seeds at a reasonable cost, especially to smallholder farmers. Supporting cotton production with appropriate extension services and training of farmers and other relevant stakeholders for best practices is required to scaling-up the use of Bt cotton in the country. Developing innovative partnerships with technology developers to enable local Bt cotton hybrid seeds production will help to achieve affordable and sustainable access to GM technology.
4.2.4 Public perception and acceptance of GMOs
There is no clear data concerning the changes in the public acceptance of GM technologies in Ethiopia. However, the transition at policy and political levels is remarkable; from a stance of “GMO free” advocacy to one with pragmatic consideration to taking advantage of changes and prospects at the global level. The public perception is expected to evolve considerably due to growing global biotechnology importance in promoting food security in the wake of climate change. However, the recent movement following a report by the USDA that recognizes Ethiopia’s commitment to implementing the amended protocol and embarking on some GM crops, has sparked severe criticisms against GMOs development in the country [44]. There has been a steep rise in anti-GMO comments following the USDA announcement [45]. It requires to provide the right information to the public and creating the right and positive public perceptions to help the right policy measures and institutional function with respect to biotechnology products.
4.3 Kenya
4.3.1 Country progress
Kenya is among the first African countries that signed the Cartagena Protocol on Biosafety in 2002. It also set up a national biosafety regulatory authority followed by a Biosafety policy signed into law in 2010 [46]. The exercise of dealing with GM products has seen many challenges such as the one when the government through the Ministry of Health instituted a Moratorium on the import and trade of GMOs on November 21, 2012, an embargo that remains in force to this day [47].
To date, two crops have been approved for commercialization use in Kenya and these are the Bt cotton hybrid, which was commercialized in 2020, and the improved cassava variety for resistance to Cassava Brown Streak Disease (CBSD). The NBA approved the application for environmental release for GM cassava containing Event 4046 in 2021 [48]. The GM cassava has increased root quality and higher yields [49]. Kenya is the first country globally to consider a request for environmental release involving GM cassava crops. Many other crops are now at different stages of regulatory approval. In the year 2021, 36 applications have been submitted for various crops under review [48].
4.3.2 Farmers access to new agricultural technologies
Kenya’s GMO regulatory framework is robust and active. It is designed for regulating contained use, import, export and transit, environmental release, and labeling [46]. The emerging research area of gene-editing technologies in food and agriculture presents the newest frontier in the area of legislation and regulations in Kenya [46]. The NBA board has undergone timely training to equip them with knowledge on the understanding of the regulatory process of genome-edited organisms and products in Kenya [46].
4.3.3 Challenges in product commercialization
A strict and arduous regulatory approval framework remains one of the most important challenges to GMO adoption in Kenya [50]. So far, Bt cotton has been commercialized and the status of Bt-maize is at the NPT stage. Access to Bt cotton hybrid seeds, access to credit to purchase Bt cotton seeds, and lack of adequate monitoring data for Bt cotton is the weak side of the commercialization process.
Among the public institutions, Government Counties can play a role by forming cotton-producing clusters to support access to Bt cotton hybrid seed and inputs and access to the cotton market to encourage cotton-producing smallholders. This exercise on Bt cotton can also be helpful for similar efforts in the future for other new technologies [51].
4.3.4 Public perception and acceptance of GMOs
Public perception of GMOs in Kenya has been mostly negative for a long time due to bad press and negative publicity about GM products [50]. Kenya had instituted a moratorium on GMO import and trade in 2012 based on a study by Séralini et al. [52] that has since been disapproved. The damage, however, had been done and slowed progress in GM acceptance and adoption in the country. For most of the public, GMOs were dangerous, and disposed the government to take a reactive action. The growing awareness on the benefits of GMO technology in the continent and in Kenya in particular, is seeing an upsurge in attitude change for the better [50].
4.4 Malawi
4.4.1 Country progress
Malawi has made significant progress in biotechnology and biosafety since the ratification of the Cartagena Protocol on Biosafety in 2009. The country has domesticated the protocol by developing a legal and institutional framework for biosafety. Malawi developed its Biosafety Act in 2002, Biosafety Regulations in 2007, and enacted Biotechnology and Biosafety Policy in 2008. The CFT and NPT Guidelines, Trial Manager Handbook, and Inspectors Handbook were prepared in 2007. Since 2009, three permits to conduct GM crop trials have been issued under the Biosafety Act and approved its first Bt cotton for commercialization in 2018. Other GM crops initiatives were transgenic Banana and Bt Cowpea both of which were terminated in 2019 due to lack of finance to support the research.
4.4.2 Farmers access to new agricultural technologies
Malawi’s biosafety legal framework does not hinder the commercialization of approved technologies. Before varietal release of the Bt cotton hybrids, field demonstrations across key cotton-growing districts were done to help farmers with the potential of the technology (Bollgard II) and hybrid cotton varieties to help farmers build a positive perception about the benefits. However, the cost of Bollgard II hybrid cotton seeds was US$30 (MK 25,000) in 2021 became a concern. This means that for a hectare, farmers spend US$ 123.5 at a seeds rate of 4 kg/ha compared to US$ 1.2/kg for OPVs. The Bt cotton seed grown in Malawi are supplied from India and transport/import cost make seed prices higher and affects the adoption of the technology by smallholder farmers. Trainings on GM cotton seed multiplication for local farmers is underway to reduce cost on seed importation which is anticipated to result into affordable seed cost and improve its accessibility and adoption by smallholder farmers.
4.4.3 Public perception and acceptance of GMOs
In Malawi issues such as biosafety concerns, public acceptance, political will, and support influence the adoption of GM crops. Public opinion has not been contradicting to the introduction of GM cotton possibly due to the absence of known negative impacts on human health and good publicity during the field demonstration trials. There is high political will as government is working to restore the cotton industry in the country. Regulatory decisions have been science-based and risk assessment is done on case-by-case basis which has built level of trust for the technology among farmers and the public.
4.5 Nigeria
4.5.1 Country progress
Modern biotechnology regulation in Nigeria started in the early 1990s. The Convention on Biological Diversity (CBD), which Nigeria signed in 1992, identified GMOs or LMOs as a group of organisms produced by modern biotechnology that needed special attention because of their perceived adverse impacts on biodiversity and human health. Based on the Convention’s recommendation, Nigeria ratified its biosafety framework in 2002. Consequently, research practice began in modern biotechnology, along with it the biosafety legal regime became apparent. Subsequently, Biosafety Law was put in place in April 2015 giving birth to the National Biosafety Management Agency (NBMA) for the implementation of the Act which also became amended in 2019.
4.5.2 Progress in product development
To keep abreast with advancements in modern biotechnology, Nigeria developed several guidelines including for GM Food, Feed Processing, GM Mosquito, GM Trees, Birds, Fish, and other animals. The country is the first in Africa to validate Genome editing guidelines during the last quarter of 2020. Several processing permits were granted for food and feed from GM maize, soybeans, and others.
Currently, Nigeria has several R&D activities at different levels: research, testing, pipeline, and commercialization. To date, NBMA has approved CFTs for the following crops: Bio-fortified cassava enhanced with pro-vitamin A, iron, and zinc; GM cassava resistant to cassava mosaic virus, Cassava brown streak disease virus, and enhanced with iron and zinc. Also, cassava was modified for higher starch; cowpea modified for resistance against maruca, HT soybeans; GM rice modified for nitrogen use efficiency, water use efficiency, and salt tolerance and GM maize for resistance to stem borer/fall armyworm and drought tolerance. The approval for commercial release has been for GM cotton (Bollgard II) to Bayer Agriculture Nig. Ltd./Mahyco Agriculture Private Ltd. in July 2018; cowpea modified for resistance to maruca insect pest and insect-resistant/drought-tolerant maize (TELA).
4.5.3 Farmers access to new agricultural technologies
The most important regulatory constraints are related to finance and laboratory facilities. The challenge in product commercialization of GM crops, as experienced in cowpea, is meeting the seed demands of farmers. Whereas in the case of cotton, the cost of seeds is not affordable by smallholder farmers, concerted efforts are being made by various platforms such as the open forum on agricultural biotechnology (OFAB), in Africa, Nigeria Chapter in collaboration with extension agents to let farmers get the right information and advisory services on biotechnology products. Nigeria’s Biosafety Law requires mandatory labeling of products containing GM products or ingredients exceeding 4%, which restricts market access for GM products.
4.5.4 Possible pathways for commercialization
Access to improved seed is realized when the farmers can buy the seeds when they need them at an affordable price. Trust building is critical so that farmers as pragmatic as they are, have a positive attitude toward GM technology despite anti-GM campaigns and their misconceptions.
4.5.5 Perception and acceptance of GMOs
The Nigerian public has a mixed opinion about GM crops and their food products due to mixed information about the importance of biotech in promoting food security and the public concerns about its safety and health-related issues. A higher number of the public in Nigeria believe the country should domesticate the technology and build local capacity to develop GM crops [53]. For example, policymakers’ and scientists’ perception on GM technology was examined in Ghana and Nigeria using semi-structured interviews [54]. Results showed most respondents including policymakers believe the technology has great potential to solve agricultural problems. However, lack of trained personnel and weak institutional capacities present significant challenges to its wider utilization.
4.6 Sudan
4.6.1 Country progress
Sudan is a member of the Cartagena Protocol on Biosafety (CPB) since 2005. In 2010, a national biosafety law dealing with the application of modern biotechnology was issued and in 2012, Biosafety Council was formed. However, biosafety measures are only partially in place for the implementation of the Cartagena Protocol [55]. Despite such efforts by the government to develop the biosafety regulatory system, much remain to be done for the effective implementation of the protocol on biosafety [56]. The national biosafety law was amended to become “Miscellaneous Amendments Law” (Unification of Environment Councils) and officially gazetted in Sudan [57].
The first open-pollinated Bt cotton genotype (CN-C02) carrying Bt gene Cry 1A from which is a specific toxin against larvae of bollworm was introduced by China-aid Agricultural Technology Demonstration Center (CATDC) and released for commercial production under the name Seeni1 in 2012. The Seeni1 variety was fast adopted at a commercial scale from 19,300 hectares in 2012 to 61,300 hectares in 2013 [58]. In 2016, the area almost doubled to 120,630 hectares. Seeni1 occupied about 25% of the country’s total cotton cultivation area in 2012 and 97% in 2014 [59]. After the successful adoption of the first Bt cotton variety, Seeni1, another open-pollinated Bt cotton genotype from China (SCRC37) carrying the same gene of Seeni1 was released for commercial production and named Seeni2 in 2015. In the same year, two Indian Bt cotton hybrids; JKCH1947 (Hindi1) and JKCH1050 (Hindi2) carrying JKAL X-gene (Cry1Ac), were also released for commercial production [60]. The area under Hindi2 progressively increased from 7560 hectares to 33,600 hectares in 2021. The total Bt cotton cultivated area in Sudan since first commercial production in 2012 has grown to occupy about 98% of the total cotton area in 2021. In Sudan, cottonseeds represent a valuable oil and cake source. The major concern after the Bt cotton commercialization is the food safety of its byproducts; however, permissible levels for GMOs intended for direct use as food/feed needs approval from the national biosafety committee.
Recently transgenic cotton hybrid varieties carrying Cry1AC + Cry2A and glyphosate-tolerant trait (CP4 ESPS) were approved by the national biosafety technical committee in compliance with the national biosafety regulations for further testing. In Sudan, the establishment of national action plans for developing and promoting cotton exports and harmonizing its marketing policies are seen as crucial steps to restore Sudan’s position in the international cotton market.
4.6.2 Farmers access to new agricultural technologies
In Sudan, Bt cotton is the only GM crop under commercial production since 2012. Additional new transgenic cotton varieties approved by the national biosafety committee are under testing and will enrich the Bt cotton variety options. The national seed industry of transgenic crops is not fully complying with the biosafety regulations due to the limited awareness of stakeholders involved in the seed industry. This has caused the sub-standard seed to be distributed by dealers.
Almost all Bt cotton seeds for open-pollinated variety are produced by the private seed sector under the governance of public institutions. The current situation of seed production could be improved with policy to guide and incentivize seed producers (public and private) for high-quality seed supply. The trend of seed demand growth in Sudan has been clear since Bt cotton adoption and requires comprehensive situation analysis to install a visionary seed production scheme.
On the other hand, not all smallholder farmers can access good quality seed because of limited financial support and a lack of farmers’ organizations to obtain agricultural credit. Enabling policies are required for smallholder cotton farmers to overcome this problem and related marketing challenges.
4.6.3 Public perception and acceptance of GMOs
Sudanese public participation in GMOs use debates and its general awareness is limited. Either lack of understanding or misperception of the technology predominates. Public-wide formal and informal education on safety concerns (biosafety and food safety) and GMO utilization need to be strengthened. More engagement and participation of stakeholders along the cotton value chain would help to have a clear plan for promoting and sustainability utilizing the products of GM technology. Currently, the adoption of transgenic cotton in Sudan is farmer-driven and government intervention is highly beneficial to strengthen farmers’ associations for market access and improving the benefits of Bt cotton to local farmers.
4.7 Uganda
4.7.1 Country progress
For the past 15 years, Uganda has been steadily integrating biotechnology into national development processes and developing local capacity. The Uganda national biotechnology strategy identified biotechnology as a tool to address challenges in the agricultural sector [61, 62]. The government has been providing support to build human resources and research infrastructure capacity to strengthen research development and innovation in biotechnology and played a dominant role in Uganda. R&D using modern biotechnology tools in crop science was initiated in 2003 at the National Agricultural Biotechnology Center. Other institutions like Makerere University and the National Agricultural Research Organization’s (NARO) followed suit to join the effort. Several international and regional organizations also have been supporting national crop biotechnology R&D including USAID, Bill and Melinda Gates Foundation, ASARECA, CIMMYT, and Rockefeller Foundation. Through support from the government and development agencies, more than 10 research laboratories have been established for biotechnology research and development. The scientific community in Uganda has embraced biotechnology and is actively engaged in R&D using modern biotechnology and genetic engineering tools. There has been a growing application of tissue culture, molecular diagnostic tools, and the development of genetically engineered transgenic crops.
4.7.2 Biosafety regulatory system
Uganda ratified the Cartagena Protocol on Biosafety in 2001 [63]. In 2008, the government of Uganda adopted the National Biotechnology and Biosafety Policy to provide a regulatory and institutional framework for the safe and sustainable application of biotechnology for national development. Uganda’s biosafety institutional framework includes national competent authority, national focal point, the national biosafety committee, monitoring and compliance mechanisms, and institutional biosafety committees.
The Uganda National Council for Science and Technology (UNCST) serves as the national competent authority and provides regulatory oversight for GMO research and development programs through the National Biosafety Committee (NBC). To support the NBC, biotechnology research institutions have established Institutional Biosafety Committees (IBC) to provide research biosafety stewardship and serve as a link between the research scientists and NBC. To provide a comprehensive biosafety regulatory framework for commercialization of GM crops, the Parliament of Uganda introduced the Genetic Engineering Regulatory Bill in November 2018 to be assented into an act. The Bill was seconded through stakeholder policy consultations to ensure establishment of an enabling national biosafety legislation.
4.7.3 Country progress
The first field trial of GM crops was conducted in 2007 on genetically engineered bananas for resistant to Black Sigatoka disease. To date, the NBC has approved 17 field research trials involving several GM crops mentioned below for various crops and traits (Table 2) [64, 65, 66]. The detailed summary of GM crops and incorporated traits is also partly presented in Table 2.
Like other breeding product pipelines, GM products require on-farm agronomic and agroecological tests under the guidance of approved biosafety guidelines. In Uganda, scientists are unable to proceed with product testing on farmer’s fields to ascertain GM product performance due to a lack of national biosafety legislation and regulations. Crops such as banana (research, CFT and multilocation trials), Cassava (CFT, multi-locational trials), Cotton (CFT, multi-location trials), Maize (CFT and multi-location trials), Rice (CFT Research), Sweet potato (Greenhouse), Soybean (Greenhouse), Potato (CFT- Multilocation trials) have not been tested on farmers fields. Research on these crops has been conducted through joint collaborations involving local and international institutions such as NARO, IITA, AATF, Queensland University of Technology (QUT), Leeds University, Donald Danforth Plant Science Center (DDPSC), Bayer, International Potato Center (CIP), Makerere University, and Michigan State University.
5. Lessons learned and future prospects
Biotechnologies can help African country’s efforts toward achieving social and economic development and contributing to the United National (UN) Sustainable Development Goals (SDGs) through improving agricultural productivity and increasing resilience to climate change impacts. As highlighted in the six case studies, countries in Africa are at various stages of biotechnology R&D and regulatory capacities. With the recent positive decisions made by the governments of several countries in Africa, the future holds prospects for the commercialization of GM products. Research, regulatory, and outreach capacity in modern biotechnology is seen as fundamental to the promotion of advanced science and technology in research programs including GMO and genome-editing research and development.
Identifying policy and regulatory gaps and adjusting to meet current and future needs would always be required to promote agricultural biotechnology for sustainable development in biotech and non-biotech countries. Proactively working toward building awareness of stakeholders and right public perception and relentless effort to capacitate policymakers would help to maintain the current efforts in improving political dynamics toward modern biotechnology and avoid sliding back to the old rhetoric led by postmodernist anti-GMO and anti-technology activism.
Since it took several years of negative publicity to entrench distrust among the public, it can only be undone with unyielding and consistent communication and outreach espousing, especially positive benefits to smallholder farmers and consumers and farmers as champions. Therefore, strong voices are necessary to champion the adoption of GMOs and genome-editing technologies in countries in Africa. Misinformation and disinformation, and competing interests inevitably complicate how modern biotechnology is viewed and its benefits are harnessed in Africa for smallholder farmers. The science communication should be amplified with messaging centering around a farmer and consumer benefits and contributions to UN Sustainable Development Goals (SDGs).
The transitions from product development to deployment and commercialization are often difficult in developing countries. Multiple institutions from the public and private sector including the farming communities are involved to operate. This needs to be well aligned and coordinated institutional functions are needed to ensure sustainable access and deployment of new technologies/products by smallholder farmers while keeping product integrity, quality, and excellence through stewardship. Experience shows the importance of careful handling and management of new technology with simultaneous preparation for the local seed systems to ensure that new products are consistently available and affordable by smallholder farmers. Alternative technologies are needed for widening the scope of adoption through a healthy market and avoiding negative perceptions to impinge on efficiency and competitiveness.
Farmers are willing to adopt impactful technologies that can enhance agricultural productivity and their livelihoods. However, closer consultation and understanding of their challenges is critical to foster and sustain repeated adoption of GM crops by farmers to convey a realistic understanding of the production and marketing challenges and receive necessary policy support. A clear monitoring strategy is needed for field management of GM crops and their sustainable use and impacts as well as co-existence in the farming systems of adopting countries.
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The most important management practice is nutrient management, which is supported by the effective use of nano-technology, especially nano-fertilizers. It is well known that nano-fertilizers are nutrient carriers of nano dimensions ranging from 30 to 40 nm (10−9 m or one-billionth of a meter). Due to their high surface area, they can hold abundant nutrients ions and release them slowly and steadily, commensurate with crop demand. Nano-fertilizers are easily uptaken and assimilated by the plants because of their ease of solubility, stability, controlled release in time, and easy mode of delivery and disposal. Due to nano fertilizers characteristics, different commercial products are available in the market, namely Nanogro, Geohumus, NanoGreen, and Lithovit High Yield fertilizer, which can be demonstrated among the farmers for increasing agricultural performance through soil and nutrient management. Besides, nano-fertilizer has good criteria like disease resistance properties. 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Theory and Applications",slug:"electrodialysis-technology-theory-and-applications",totalDownloads:14567,totalCrossrefCites:23,totalDimensionsCites:61,abstract:null,book:{id:"51",slug:"desalination-trends-and-technologies",title:"Desalination",fullTitle:"Desalination, Trends and Technologies"},signatures:"Fernando Valero, Angel Barceló and Ramón Arbós",authors:[{id:"17419",title:"Dr.",name:"Fernando",middleName:null,surname:"Valero-Cervera",slug:"fernando-valero-cervera",fullName:"Fernando Valero-Cervera"},{id:"18893",title:"Dr.",name:"Ramón",middleName:null,surname:"Arbós-Sans",slug:"ramon-arbos-sans",fullName:"Ramón Arbós-Sans"},{id:"47900",title:"Prof.",name:"Angel",middleName:null,surname:"Barceló",slug:"angel-barcelo",fullName:"Angel Barceló"}]},{id:"33763",doi:"10.5772/37625",title:"Membrane Distillation: Principle, Advances, Limitations and Future Prospects in Food Industry",slug:"membrane-distillation-principle-advances-limitations-and-future-prospects-in-food-industry",totalDownloads:10132,totalCrossrefCites:17,totalDimensionsCites:43,abstract:null,book:{id:"1981",slug:"distillation-advances-from-modeling-to-applications",title:"Distillation",fullTitle:"Distillation - Advances from Modeling to Applications"},signatures:"Pelin Onsekizoglu",authors:[{id:"113560",title:"Dr.",name:"Pelin",middleName:null,surname:"Onsekizoglu",slug:"pelin-onsekizoglu",fullName:"Pelin Onsekizoglu"}]},{id:"42247",doi:"10.5772/53822",title:"Application of Differential Scanning Calorimetry to the Characterization of Biopolymers",slug:"application-of-differential-scanning-calorimetry-to-the-characterization-of-biopolymers",totalDownloads:8884,totalCrossrefCites:1,totalDimensionsCites:37,abstract:null,book:{id:"2519",slug:"applications-of-calorimetry-in-a-wide-context-differential-scanning-calorimetry-isothermal-titration-calorimetry-and-microcalorimetry",title:"Applications of Calorimetry in a Wide Context",fullTitle:"Applications of Calorimetry in a Wide Context - Differential Scanning Calorimetry, Isothermal Titration Calorimetry and Microcalorimetry"},signatures:"Adriana Gregorova",authors:[{id:"142120",title:"Dr",name:"Adriana",middleName:null,surname:"Gregorova",slug:"adriana-gregorova",fullName:"Adriana Gregorova"}]},{id:"42261",doi:"10.5772/51882",title:"Differential Scanning Calorimetry Studies of Phospholipid Membranes: The Interdigitated Gel Phase",slug:"differential-scanning-calorimetry-studies-of-phospholipid-membranes-the-interdigitated-gel-phase",totalDownloads:4636,totalCrossrefCites:3,totalDimensionsCites:35,abstract:null,book:{id:"2519",slug:"applications-of-calorimetry-in-a-wide-context-differential-scanning-calorimetry-isothermal-titration-calorimetry-and-microcalorimetry",title:"Applications of Calorimetry in a Wide Context",fullTitle:"Applications of Calorimetry in a Wide Context - Differential Scanning Calorimetry, Isothermal Titration Calorimetry and Microcalorimetry"},signatures:"Eric A. Smith and Phoebe K. Dea",authors:[{id:"139501",title:"Prof.",name:"Phoebe",middleName:null,surname:"Dea",slug:"phoebe-dea",fullName:"Phoebe Dea"},{id:"141802",title:"Mr.",name:"Eric",middleName:null,surname:"Smith",slug:"eric-smith",fullName:"Eric Smith"}]},{id:"13758",doi:"10.5772/14779",title:"Renewable Energy Opportunities in Water Desalination",slug:"renewable-energy-opportunities-in-water-desalination",totalDownloads:7436,totalCrossrefCites:4,totalDimensionsCites:33,abstract:null,book:{id:"51",slug:"desalination-trends-and-technologies",title:"Desalination",fullTitle:"Desalination, Trends and Technologies"},signatures:"Ali A. Al-Karaghouli and L.L. Kazmerski",authors:[{id:"18734",title:"Prof.",name:"Ali A.",middleName:null,surname:"Al-Karaghouli",slug:"ali-a.-al-karaghouli",fullName:"Ali A. Al-Karaghouli"}]}],mostDownloadedChaptersLast30Days:[{id:"44033",title:"Ion-Exchange Chromatography and Its Applications",slug:"ion-exchange-chromatography-and-its-applications",totalDownloads:29735,totalCrossrefCites:7,totalDimensionsCites:26,abstract:null,book:{id:"3487",slug:"column-chromatography",title:"Column Chromatography",fullTitle:"Column Chromatography"},signatures:"Özlem Bahadir Acikara",authors:[{id:"109364",title:"Dr.",name:"Özlem",middleName:null,surname:"Bahadır Acıkara",slug:"ozlem-bahadir-acikara",fullName:"Özlem Bahadır Acıkara"}]},{id:"53750",title:"Mathematical Modelling of Batch Distillation Columns: A Comparative Analysis of Non-Linear and Fuzzy Models",slug:"mathematical-modelling-of-batch-distillation-columns-a-comparative-analysis-of-non-linear-and-fuzzy-",totalDownloads:2406,totalCrossrefCites:2,totalDimensionsCites:3,abstract:"Distillation is the process most commonly used in industry to separate chemical mixtures; its applications range from cosmetic and pharmaceutical to petrochemical industries. The equipment required to perform the distillation process is known as distillation column. Since initial investment and maintenance costs for distillation columns are very high it is necessary to have an appropriate mathematical model that allows improving the comprehension of the column dynamics, especially its thermal behaviour, in order to enhance the control and safety of the process. This chapter presents a general panorama of the mathematical modelling of distillation columns, having as a specific case of study the comparison of a space-state non-linear model and a Takagi-Sugeno fuzzy model for a batch distillation column using a binary mixture (Ethanol-Water).",book:{id:"5452",slug:"distillation-innovative-applications-and-modeling",title:"Distillation",fullTitle:"Distillation - Innovative Applications and Modeling"},signatures:"Adriana del Carmen Téllez-Anguiano, Mario Heras-Cervantes, Juan\nAnzurez-Marín, Gerardo Marx Chávez-Campos and José Antonio\nGutiérrez Gnecchi",authors:[{id:"12387",title:"Dr.",name:"Jose Antonio",middleName:null,surname:"Gutierrez Gnecchi",slug:"jose-antonio-gutierrez-gnecchi",fullName:"Jose Antonio Gutierrez Gnecchi"},{id:"189166",title:"Dr.",name:"Adriana",middleName:null,surname:"Téllez-Anguiano",slug:"adriana-tellez-anguiano",fullName:"Adriana Téllez-Anguiano"},{id:"194844",title:"MSc.",name:"Mario",middleName:null,surname:"Heras-Cervantes",slug:"mario-heras-cervantes",fullName:"Mario Heras-Cervantes"},{id:"194845",title:"Dr.",name:"Juan",middleName:null,surname:"Anzurez-Marín",slug:"juan-anzurez-marin",fullName:"Juan Anzurez-Marín"},{id:"194846",title:"Dr.",name:"Gerardo",middleName:"Marx",surname:"Chávez-Campos",slug:"gerardo-chavez-campos",fullName:"Gerardo Chávez-Campos"}]},{id:"54078",title:"Distillation Techniques in the Fruit Spirits Production",slug:"distillation-techniques-in-the-fruit-spirits-production",totalDownloads:4944,totalCrossrefCites:13,totalDimensionsCites:24,abstract:"During the distillation of the fermented fruit mash or juice, ethanol and water are the carriers of a huge number of the other volatile aroma compounds. Unique and distinctive flavour of the final spirits depends on their quantity and quality. Fruit spirits have higher concentration of almost all types of volatile compounds with comparing to other types of distilled spirits. The art of distillation run is to obtain the best balance between congeners present. Two different types of distillation equipment are used for the production of fruit spirits: copper Charentais alembic and batch distillation columns. Although both distillation methods are based on the same theoretical principles, a different quantity of the flavour compounds of the final spirits is produced by using different distillation equipment. The main difference was shown in different distributions of the methanol, n-propanol, higher alcohols and fatty acid esters. Distillation methods need to be adjusted for each fruit spirits regardless to distillation equipment employed because fermented mash of different fruit varieties has a different requirement for distilling. Alembic stills yield better aroma and more characteristic fruit distillates but are slow and require more labour. Column still cleans the distillate giving a decent aroma and higher concentration of alcohol.",book:{id:"5452",slug:"distillation-innovative-applications-and-modeling",title:"Distillation",fullTitle:"Distillation - Innovative Applications and Modeling"},signatures:"Nermina Spaho",authors:[{id:"189124",title:"Associate Prof.",name:"Nermina",middleName:null,surname:"Spaho",slug:"nermina-spaho",fullName:"Nermina Spaho"}]},{id:"54676",title:"Fractional Distillation of Organic Liquid Compounds Produced by Catalytic Cracking of Fats, Oils, and Grease",slug:"fractional-distillation-of-organic-liquid-compounds-produced-by-catalytic-cracking-of-fats-oils-and-",totalDownloads:1743,totalCrossrefCites:0,totalDimensionsCites:2,abstract:"This work aims to investigate the fractional distillation of organic liquid products (OLP) obtained by catalytic cracking of palm oil (Elaeis guineensis Jacq.) at 450°C, 1.0 atm, with 5, 10, and 15% (wt) Na2CO3, using a stirred tank reactor of 143 L. The fractional distillations of OLP were carried out in laboratory scale with and without reflux using columns of different heights, and a pilot‐packed distillation column with internal reflux. OLP and distillation fractions (gasoline, kerosene, light diesel, and heavy diesel) were physicochemically characterized for density, kinematic viscosity, acid value, saponification value, refractive index, flash point, and copper strip corrosion. The OLP and light diesel fractions were analyzed by Fourier transform infrared spectroscopy (FT‐IR) and gas chromatography‐mass spectrometry (GC‐MS). For the experiments in laboratory scale, the yields of distillates decrease along with column height, with and without reflux, while those of bottoms products increase. The yields of distillates and gas increase with increasing Na2CO3 content, while those of bottoms products decrease. The densities of gasoline, kerosene, and light diesel produced in laboratory scale with reflux superpose exactly those of kerosene, light diesel, and heavy diesel produced in laboratory scale without reflux. The kinematic viscosity decreases with increasing column height for the experiments in laboratory scale. The acid values of distillation fractions decrease along with the column height for the experiments with and without reflux. The FT‐IR of distillation fractions in pilot and laboratory scales identified the presence of aliphatic hydrocarbons and oxygenates. The GC‐MS analysis identified OLP composition of 92.84% (area) hydrocarbons and 7.16% (area) oxygenates. The light diesel fraction contains 100% hydrocarbons with an acid value of 0.34 mg KOH/g, proving the technical feasibility of OLP de‐acidification by the fractional distillation process.",book:{id:"5452",slug:"distillation-innovative-applications-and-modeling",title:"Distillation",fullTitle:"Distillation - Innovative Applications and Modeling"},signatures:"C. C. Fereira, E. C. Costa, D. A. R. de Castro, M. S. Pereira, A. A.\nMâncio, M. C. Santos, D. E. L. Lhamas, S. A. P. da Mota, M. E. Araújo,\nLuiz E. P. Borges and N. T. Machado",authors:[{id:"189087",title:"Prof.",name:"Nelio",middleName:null,surname:"Teixeira Machado",slug:"nelio-teixeira-machado",fullName:"Nelio Teixeira Machado"}]},{id:"43603",title:"Ion Exchange Chromatography - An Overview",slug:"ion-exchange-chromatography-an-overview",totalDownloads:5941,totalCrossrefCites:1,totalDimensionsCites:3,abstract:null,book:{id:"3487",slug:"column-chromatography",title:"Column Chromatography",fullTitle:"Column Chromatography"},signatures:"Yasser M. Moustafa and Rania E. 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Much of biochemistry is devoted to enzymes, proteins that catalyze chemical reactions, enzyme structures, mechanisms of action and their roles within cells. Biochemistry also studies small signaling molecules, coenzymes, inhibitors, vitamins, and hormones, which play roles in life processes. Biochemical experimentation, besides coopting classical chemistry methods, e.g., chromatography, adopted new techniques, e.g., X-ray diffraction, electron microscopy, NMR, radioisotopes, and developed sophisticated microbial genetic tools, e.g., auxotroph mutants and their revertants, fermentation, etc. More recently, biochemistry embraced the ‘big data’ omics systems. Initial biochemical studies have been exclusively analytic: dissecting, purifying, and examining individual components of a biological system; in the apt words of Efraim Racker (1913 –1991), “Don’t waste clean thinking on dirty enzymes.” Today, however, biochemistry is becoming more agglomerative and comprehensive, setting out to integrate and describe entirely particular biological systems. The ‘big data’ metabolomics can define the complement of small molecules, e.g., in a soil or biofilm sample; proteomics can distinguish all the comprising proteins, e.g., serum; metagenomics can identify all the genes in a complex environment, e.g., the bovine rumen. 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Dr. Blumenberg’s research is focused on the epidermis, expression of keratin genes, transcription profiling, keratinocyte differentiation, inflammatory diseases and cancers, and most recently the effects of the microbiome on the skin. He has published more than 100 peer-reviewed research articles and graduated numerous Ph.D. and postdoctoral students.",institutionString:null,institution:{name:"New York University Langone Medical Center",institutionURL:null,country:{name:"United States of America"}}},editorTwo:null,editorThree:null},subseries:{paginationCount:3,paginationItems:[{id:"7",title:"Bioinformatics and Medical Informatics",coverUrl:"https://cdn.intechopen.com/series_topics/covers/7.jpg",isOpenForSubmission:!0,editor:{id:"351533",title:"Dr.",name:"Slawomir",middleName:null,surname:"Wilczynski",slug:"slawomir-wilczynski",fullName:"Slawomir Wilczynski",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y000035U1loQAC/Profile_Picture_1630074514792",biography:"Professor Sławomir Wilczyński, Head of the Chair of Department of Basic Biomedical Sciences, Faculty of Pharmaceutical Sciences, Medical University of Silesia in Katowice, Poland. His research interests are focused on modern imaging methods used in medicine and pharmacy, including in particular hyperspectral imaging, dynamic thermovision analysis, high-resolution ultrasound, as well as other techniques such as EPR, NMR and hemispheric directional reflectance. Author of over 100 scientific works, patents and industrial designs. Expert of the Polish National Center for Research and Development, Member of the Investment Committee in the Bridge Alfa NCBiR program, expert of the Polish Ministry of Funds and Regional Policy, Polish Medical Research Agency. Editor-in-chief of the journal in the field of aesthetic medicine and dermatology - Aesthetica.",institutionString:null,institution:{name:"Medical University of Silesia",institutionURL:null,country:{name:"Poland"}}},editorTwo:null,editorThree:null},{id:"8",title:"Bioinspired Technology and Biomechanics",coverUrl:"https://cdn.intechopen.com/series_topics/covers/8.jpg",isOpenForSubmission:!0,editor:{id:"144937",title:"Prof.",name:"Adriano",middleName:"De Oliveira",surname:"Andrade",slug:"adriano-andrade",fullName:"Adriano Andrade",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRC8QQAW/Profile_Picture_1625219101815",biography:"Dr. Adriano de Oliveira Andrade graduated in Electrical Engineering at the Federal University of Goiás (Brazil) in 1997. He received his MSc and PhD in Biomedical Engineering respectively from the Federal University of Uberlândia (UFU, Brazil) in 2000 and from the University of Reading (UK) in 2005. He completed a one-year Post-Doctoral Fellowship awarded by the DFAIT (Foreign Affairs and International Trade Canada) at the Institute of Biomedical Engineering of the University of New Brunswick (Canada) in 2010. Currently, he is Professor in the Faculty of Electrical Engineering (UFU). He has authored and co-authored more than 200 peer-reviewed publications in Biomedical Engineering. He has been a researcher of The National Council for Scientific and Technological Development (CNPq-Brazil) since 2009. He has served as an ad-hoc consultant for CNPq, CAPES (Coordination for the Improvement of Higher Education Personnel), FINEP (Brazilian Innovation Agency), and other funding bodies on several occasions. He was the Secretary of the Brazilian Society of Biomedical Engineering (SBEB) from 2015 to 2016, President of SBEB (2017-2018) and Vice-President of SBEB (2019-2020). 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Science",numberOfPublishedBooks:10,numberOfPublishedChapters:103,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2632-0517",doi:"10.5772/intechopen.73681",isOpenForSubmission:!0}],sshSeriesList:[{id:"22",title:"Business, Management and Economics",numberOfPublishedBooks:1,numberOfPublishedChapters:12,numberOfOpenTopics:2,numberOfUpcomingTopics:1,issn:null,doi:"10.5772/intechopen.100359",isOpenForSubmission:!0},{id:"23",title:"Education and Human Development",numberOfPublishedBooks:0,numberOfPublishedChapters:0,numberOfOpenTopics:2,numberOfUpcomingTopics:0,issn:null,doi:"10.5772/intechopen.100360",isOpenForSubmission:!1},{id:"24",title:"Sustainable Development",numberOfPublishedBooks:0,numberOfPublishedChapters:10,numberOfOpenTopics:4,numberOfUpcomingTopics:1,issn:null,doi:"10.5772/intechopen.100361",isOpenForSubmission:!0}],subseriesList:[{id:"4",title:"Fungal Infectious Diseases",scope:"Fungi are ubiquitous and there are almost no non-pathogenic fungi. Fungal infectious illness prevalence and prognosis are determined by the exposure between fungi and host, host immunological state, fungal virulence, and early and accurate diagnosis and treatment. \r\nPatients with both congenital and acquired immunodeficiency are more likely to be infected with opportunistic mycosis. Fungal infectious disease outbreaks are common during the post- disaster rebuilding era, which is characterised by high population density, migration, and poor health and medical conditions.\r\nSystemic or local fungal infection is mainly associated with the fungi directly inhaled or inoculated in the environment during the disaster. The most common fungal infection pathways are human to human (anthropophilic), animal to human (zoophilic), and environment to human (soilophile). Diseases are common as a result of widespread exposure to pathogenic fungus dispersed into the environment. \r\nFungi that are both common and emerging are intertwined. In Southeast Asia, for example, Talaromyces marneffei is an important pathogenic thermally dimorphic fungus that causes systemic mycosis. Widespread fungal infections with complicated and variable clinical manifestations, such as Candida auris infection resistant to several antifungal medicines, Covid-19 associated with Trichoderma, and terbinafine resistant dermatophytosis in India, are among the most serious disorders. \r\nInappropriate local or systemic use of glucocorticoids, as well as their immunosuppressive effects, may lead to changes in fungal infection spectrum and clinical characteristics. Hematogenous candidiasis is a worrisome issue that affects people all over the world, particularly ICU patients. CARD9 deficiency and fungal infection have been major issues in recent years. Invasive aspergillosis is associated with a significant death rate. Special attention should be given to endemic fungal infections, identification of important clinical fungal infections advanced in yeasts, filamentous fungal infections, skin mycobiome and fungal genomes, and immunity to fungal infections.\r\nIn addition, endemic fungal diseases or uncommon fungal infections caused by Mucor irregularis, dermatophytosis, Malassezia, cryptococcosis, chromoblastomycosis, coccidiosis, blastomycosis, histoplasmosis, sporotrichosis, and other fungi, should be monitored. \r\nThis topic includes the research progress on the etiology and pathogenesis of fungal infections, new methods of isolation and identification, rapid detection, drug sensitivity testing, new antifungal drugs, schemes and case series reports. It will provide significant opportunities and support for scientists, clinical doctors, mycologists, antifungal drug researchers, public health practitioners, and epidemiologists from all over the world to share new research, ideas and solutions to promote the development and progress of medical mycology.",coverUrl:"https://cdn.intechopen.com/series_topics/covers/4.jpg",keywords:"Emerging Fungal Pathogens, Invasive Infections, Epidemiology, Cell Membrane, Fungal Virulence, Diagnosis, Treatment"},{id:"5",title:"Parasitic Infectious Diseases",scope:"Parasitic diseases have evolved alongside their human hosts. In many cases, these diseases have adapted so well that they have developed efficient resilience methods in the human host and can live in the host for years. Others, particularly some blood parasites, can cause very acute diseases and are responsible for millions of deaths yearly. Many parasitic diseases are classified as neglected tropical diseases because they have received minimal funding over recent years and, in many cases, are under-reported despite the critical role they play in morbidity and mortality among human and animal hosts. The current topic, Parasitic Infectious Diseases, in the Infectious Diseases Series aims to publish studies on the systematics, epidemiology, molecular biology, genomics, pathogenesis, genetics, and clinical significance of parasitic diseases from blood borne to intestinal parasites as well as zoonotic parasites. We hope to cover all aspects of parasitic diseases to provide current and relevant research data on these very important diseases. In the current atmosphere of the Coronavirus pandemic, communities around the world, particularly those in different underdeveloped areas, are faced with the growing challenges of the high burden of parasitic diseases. At the same time, they are faced with the Covid-19 pandemic leading to what some authors have called potential syndemics that might worsen the outcome of such infections. Therefore, it is important to conduct studies that examine parasitic infections in the context of the coronavirus pandemic for the benefit of all communities to help foster more informed decisions for the betterment of human and animal health.",coverUrl:"https://cdn.intechopen.com/series_topics/covers/5.jpg",keywords:"Blood Borne Parasites, Intestinal Parasites, Protozoa, Helminths, Arthropods, Water Born Parasites, Epidemiology, Molecular Biology, Systematics, Genomics, Proteomics, Ecology"},{id:"6",title:"Viral Infectious Diseases",scope:"The Viral Infectious Diseases Book Series aims to provide a comprehensive overview of recent research trends and discoveries in various viral infectious diseases emerging around the globe. The emergence of any viral disease is hard to anticipate, which often contributes to death. A viral disease can be defined as an infectious disease that has recently appeared within a population or exists in nature with the rapid expansion of incident or geographic range. This series will focus on various crucial factors related to emerging viral infectious diseases, including epidemiology, pathogenesis, host immune response, clinical manifestations, diagnosis, treatment, and clinical recommendations for managing viral infectious diseases, highlighting the recent issues with future directions for effective therapeutic strategies.",coverUrl:"https://cdn.intechopen.com/series_topics/covers/6.jpg",keywords:"Novel Viruses, Virus Transmission, Virus Evolution, Molecular Virology, Control and Prevention, Virus-host Interaction"}],annualVolumeBook:{},thematicCollection:[],selectedSeries:{title:"Infectious Diseases",id:"6"},selectedSubseries:null},seriesLanding:{item:null},libraryRecommendation:{success:null,errors:{},institutions:[]},route:{name:"profile.detail",path:"/profiles/330145",hash:"",query:{},params:{id:"330145"},fullPath:"/profiles/330145",meta:{},from:{name:null,path:"/",hash:"",query:{},params:{},fullPath:"/",meta:{}}}},function(){var e;(e=document.currentScript||document.scripts[document.scripts.length-1]).parentNode.removeChild(e)}()