Microbial Insecticides: A summary of products and their uses.
\r\n\tThe main goal of this book is (1) prevention and treatment of life-related diseases and metabolic syndrome, (2) mechanism of lifestyle-related diseases and metabolic syndrome, (3) effective functional compounds, foods, and diet to lifestyle-related diseases and metabolic syndrome, (4) relationship between metabolic syndrome and various diseases such as cancer, allergy, and aging. This book hopes to bring an overview of the recent advances of metabolic syndrome, and thus we welcome both review chapters and original chapters.
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There has been an increased interest in biological control agents in last decade. More number of biocontrol agents was screened for their efficacy and environmental impact including mammalian safety. Many organisms have been investigated as potential agents for vector mosquito control, including viruses, fungi, bacteria, protozoa, nematodes, invertebrate predators and fish. However, most of these agents were shown to be of little operational use, largely because of the difficulty in multiplying them in large quantities. Some species of organisms, those that have been introduced from elsewhere may be pest to other organisms as well. They are pests to the extend which efforts must have been made to control them both in terrestrial and aquatic/freshwater environments [1]. Prior to the advent of chemical pesticides, predators which are natural enemies of those specific pests, were an important subject in biological sciences with respect to agriculture and forest pest control.
Pesticides that include insecticides, herbicides, and fungicides are employed in modern agriculture to control pests and to increase crop yield. In both developed and developing countries, the use of chemical pesticides has increased dramatically during the last few decades. Control of pests with synthetic chemicals results in several problems. The residues ofthese synthetic insecticides cause toxic effects on wild life (e.g.,birds, beneficial insects like honeybees). These chemical insecticides also induce harmful chemical changes on non-target insects/pests on their predators, parasites, etc. They can also be harmful to humans and domestic animals. Other environmental concern is the contamination of ground water [2]
In addition, there have been several recent research on biological control of marine pests [3]. The introduction of marine pests to new habitats is as old as nautical experience. Atlantic shipworms were quite possibly the first for the applicaiton of some new predator, Mytilus gallprovincialis, and the western Atlantic populations of the European green crab have planted themselves so firmly as a neutralized part of the biota. Many other introductions, such as polychaetes, amphipods are cryptic and have been considered species with natural cosmopolitan distributions [4]
Agriculture and forests form an important resource to sustain global economical, environmental and social system. For this reason, the global challenge is to secure high and quality yields and to make agricultural produce environmentally compatible. Chemical means of plant protection occupy the leading place as regards their total volume of application in integrated pest management and diseases of plants. But pesticides cause toxicity to humans and warm-blooded animals.
Despite many years of effective control by conventional agrochemical insecticides, a number of factors are threatening the effectiveness and continued use of these agents. These include the development of insecticide resistance and use-cancellation or de-registration of some insecticides due to human health and environmental concerns. Therefore, an eco-friendly alternative is the need of the hour. Improvement in pest control strategies represents one method to generate higher quality and greater quantity of agricultural products. Therefore, there is a need to develop biopesticides which are effective, biodegradable and do not leave any harmful effect on environment [5].
The biopesticides are certain types of pesticides derived from such natural materials as animals, plants, bacteria, and certain minerals. For example, canola oil and baking soda have pesticidal applications and are considered biopesticides. Even at the end of 2001, there were approximately 195 registered biopesticide active ingredients and 780 products. Biopesticides are biochemical pesticides that are naturally occurring substances that control pests by nontoxic mechanisms. They are living organisms (natural enemies) or their products (phytochemicals, microbial products) or byproducts (semiochemicals) which can be used for the management of pests that are injurious to crop plants. Biopesticides have an important role in crop protection, although most commonly in combination with other tools including chemical pesticides as part of Bio-intensive Integrated Pest Management.
They are biological pesticides based on pathogenic microorganisms specific to a target pest offer an ecologically sound and effective solution to pest problems. They pose less threat to the environment and to human health. The most commonly used biopesticides are living organisms, which are pathogenic for the pest of interest. These include biofungicides (
The advantages of using biopesticides ( in place of other chemical ones are based on these factors:
Ecological benefit; inherently less harmful and less environmental load.
Target specificity; designed to affect only one specific pest or, in somecases, a few target organisms,
Environmental beneficiency; often effective in very small quantities and often decompose quickly, thereby resulting in lower exposures and largely avoiding the pollution problems.
Suitability; when used as a component of an integrated pest management (IPM) programs, biopesticides can contribute greatly.
They come from naturally occurring or genetically altered bacteria, fungi,algae, viruses or protozoans. Microbial control agents can be effective and used as alternatives to chemical insecticides. A microbial toxin can be defined as a biological toxin material derived from a microorganism, such as a bacterium or fungus. Pathogenic effect of those microorganisms on the target pests are so species specific. The effect by microbial entomopathogens occurs by invasion through the integument or gut of the insect, followed by multiplication of the pathogen resulting in the death of the host, e.g., insects. Studies have demonstrated that the pathogens produce insecticidal toxin important in pathogenesis. Most of the toxins produced by microbial pathogens which have been identified are peptides, but they vary greatly in terms of structure, toxicity and specificity. [7].
These microbial pesticides offer an alternative to chemical insecticides with increased target specificity and ecological safety so that they are used either uniqly or in combination with other pest management programmes. One definition for integrated pest management (IPM) which is most relevant to this practice comes from Flint and van den Bosch [1981]: "It is a ecologically based pest control strategy that relies heavily on natural mortality factors and seeks out control tactics that disrupt these factors as little as possible. Ideally, an integrated pest management program considers all available pest control actions, including no action, and evaluates the potential interaction among various control tactics, cultural practices, weather, other pests, and the crop to be protected"[8].
These microbials as biocontrol agents present u beneficiancy. They have efficiency and safety for humans and other nontarget organisms. They leave less or no residue in food. They are ecologically safe, so that other natural enemies are free of their threatening, leading to preservation of other natural enemies, and increased biodiversity in managed ecosystem. So, microbial agents are highly specific against target pests so they facilitate the survival of beneficial insects in treated crops. This may be the main reason that microbial insecticides are being developed as biological control agents during the last three decades.
Microorganism e.g., a bacterium, fungus, virus or protozoan as the active ingredient can control many different kinds of pests, although each separate active ingredient is relatively specific for its target pest. For example, there are fungi that control certain weeds, and other fungi that kill specific insects. One bacterial species like
Individual products differ in important ways, but the following list of beneficial characteristics applies to microbial insecticides in general.
The organisms used in microbial insecticides are essentially nontoxic and nonpathogenic to wildlife, humans, and other organisms not closely related to the target pest. The safety offered by microbial insecticides is their greatest strength.
The toxic action of microbial insecticides is often specific to a single group or species of insects, and this specificity means that most microbial insecticides do not directly affect beneficial insects (including predators or parasites of pests) in treated areas.
If necessary, most microbial insecticides can be used in conjunction with synthetic chemical insecticides because in most cases the microbial product is not deactivated or damaged by residues of conventional insecticides. (Follow label directions concerning any limitations.)
Because their residues present no hazards to humans or other animals, microbial insecticides can be applied even when a crop is almost ready for harvest.
In some cases, the pathogenic microorganisms can become established in a pest population or its habitat and provide control during subsequent pest generations or seasons.
They also enhance the root and plant growth by way of encouraging the beneficial soil microflora. By this way they take a part in the increase of the crop yield.
Naturally there are also the limitations which are listed below, but do not prevent the successful use of microbial insecticides. These factors just provide users to choose effective microbial products and take necessary steps to achieve successful results.
Because a single microbial insecticide is toxic to only a specific species or group of insects, each application may control only a portion of the pests present in a field and garden. If other types of pests are present in the treated area, they will survive and may continue to cause damage. Conventional insecticides are subject to similar limitations because they too are not equally effective against all pests. This is because of selectivity indeed and this negative aspect is often more noticeable for both general predators, chemicals and microbials. On the other hand predators and chemicals may be danger for other beneficial insects in threatened area.
Heat, desiccation (drying out), or exposure to ultraviolet radiation reduces the effectiveness of several types of microbial insecticides. Consequently, proper timing and application procedures are especially important for some products.
Special formulation and storage procedures are necessary for some microbial pesticides. Although these procedures may complicate the production and distribution of certain products, storage requirements do not seriously limit the handling of microbial insecticides that are widely available. (Store all pesticides, including microbial insecticides, according to label directions.)
Because several microbial insecticides are pest-specific, the potential market for these products may be limited. Their development, registration, and production costs cannot be spread over a wide range of pest control sales. Consequently, some products are not widely available or are relatively expensive (several insect viruses, for example).
BACTERIA | \n\t\t|||
\n\t\t\t\t | \n\t\t\tBactur®, Bactospeine®, Bioworm®, Caterpillar Killer®, Dipel®, Futura®, Javelin®, SOK-Bt®, Thuricide®, Topside®, Tribactur®, Worthy Attack® | \n\t\t\tcaterpillars (larvae of moths and butterflies) | \n\t\t\tEffective for foliage-feeding caterpillars (and Indian meal moth in stored grain). Deactivated rapidly in sunlight; apply in the evening or on overcast days and direct some spray to lower surfaces or leaves. Does not cycle extensively in the environment. | \n\t\t
\n\t\t\t\t | \n\t\t\tAquabee®, Bactimos®, Gnatrol®, LarvX®, Mosquito Attack®, Skeetal®, Teknar®, Vectobac® | \n\t\t\tlarvae of | \n\t\t\tEffective against larvae only. Active only if ingested. | \n\t\t
\n\t\t\t\t | \n\t\t\tFoil® M-One® M-Track®, Novardo® Trident® | \n\t\t\tlarvae of Colorado potato beetle, elm leaf beetle adults | \n\t\t\tEffective against Colorado potato beetle larvae and the elm leaf beetle. Like other | \n\t\t
\n\t\t\t\t | \n\t\t\tCertan® | \n\t\t\twax moth caterpillars | \n\t\t\tUsed only for control of was moth infestations in honeybee hives. | \n\t\t
\n\t\t\t\t | \n\t\t\tDoom¨, Japidemic¨,® Milky Spore Disease, Grub Attack® | \n\t\t\tlarvae (grubs) of Japanese beetle | \n\t\t\tThe main Illinois lawn grub (the annual white grub, | \n\t\t
\n\t\t\t\t | \n\t\t\tVectolex CG®, Vectolex WDG® | \n\t\t\tlarvae of | \n\t\t\tActive only if ingested, for use against | \n\t\t
FUNGI | \n\t\t|||
\n\t\t\t\t | \n\t\t\tBotanigard®, Mycotrol®, Naturalis® | \n\t\t\taphids, fungus gnats, mealy bugs, mites, thrips, whiteflies | \n\t\t\tEffective against several pests. High moisture requirements, lack of storage longevity, and competition with other soil microorganisms are problems that remain to be solved. | \n\t\t
\n\t\t\t\t | \n\t\t\tLaginex® | \n\t\t\tlarvae of most pest mosquito species | \n\t\t\tEffective against larvae of most pest mosquito species; remains infective in the environment through dry periods. A main drawback is its inability to survive high summertime temperatures. | \n\t\t
PROTOZOA | \n\t\t|||
\n\t\t\t\t | \n\t\t\tNOLO Bait®, Grasshopper Attack® | \n\t\t\tEuropean cornborer caterpillars, grasshoppers and mormon crickets | \n\t\t\tUseful for rangeland grasshopper control. Active only if ingested. Not recommended for use on a small scale, such as backyard gardens, because the disease is slow acting and grasshoppers are very mobile. Also effective against caterpillars. | \n\t\t
VIRUSES | \n\t\t|||
Gypsy moth nuclear plyhedrosis (NPV) | \n\t\t\tGypchek® virus | \n\t\t\tgypsy moth caterpillars | \n\t\t\tAll of the viral insecticides used for control of forest pests are produced and used exclusively by the U.S. Forest Service. | \n\t\t
Tussock moth NPV | \n\t\t\tTM Biocontrol-1® | \n\t\t\ttussock moth caterpillars | \n\t\t\t\n\t\t |
Pine sawfly NPV | \n\t\t\tNeochek-S® | \n\t\t\tpine sawfly larvae | \n\t\t\t\n\t\t |
Codling moth granulosis virus (GV) | \n\t\t\t(see comments) | \n\t\t\tcodling moth caterpillars | \n\t\t\tCommercially produced and marketed briefly, but no longer registered or available. Future re-registration is possible. Subject to rapid breakdown in ultraviolet light. | \n\t\t
ENTOMOGENOUS NEMATODES | \n\t\t|||
\n\t\t\t\t | \n\t\t\tBiosafe®, Ecomask®, Scanmask®, also sold generically (wholesale and retail), Vector® | \n\t\t\tlarvae of a wide variety of soil-dwelling and boring insects | \n\t\t\t\n\t\t\t\t | \n\t\t
\n\t\t\t\t | \n\t\t\tcurrently available on a wholesale basis for large scale operations | \n\t\t\tlarvae of a wide variety of soil-dwelling and boring insects | \n\t\t\tNot commonly available by retail in the U.S.; this species is used more extensively in Europe. Available by wholesale or special order for research or large-scale commercial uses. | \n\t\t
PATHOGEN | \n\t\t|||
\n\t\t\t\t | \n\t\t\tNematac®S | \n\t\t\tlate nymph and adult stages of mole crickets | \n\t\t\t\n\t\t\t\t | \n\t\t
Microbial Insecticides: A summary of products and their uses.
(Agricultural Entomology, University of Illinois at Urbana-Champaign. ENY-275 IN081)
Entomopathogenic fungi are important natural regulators of insect populations and have potential as mycoinsecticide agents against diverse insect pests in agriculture. These fungi infect their hosts by penetrating through the cuticle, gaining access to the hemolymph, producing toxins, and grow by utilizing nutrients present in the haemocoel to avoid insect immune responses [10]. Entomopathogenic fungi may be applied in the form of conidia or mycelium which sporulates after application. The use of fungal entomopathogens as alternative to insecticide or combined application of insecticide with fungal entomopathogens could be very useful for insecticide resistant management [13].
The commercial mycoinsecticide ‘Boverin’ based on
The use of the insect-pathogenic fungus
There are more than 1600 different viruses which infect 1100 species of insects and mites. A special group of viruses, called baculovirus, to which about 100 insect species are susceptible, accounts for more than 10 percent of all insect pathogenic viruses. Baculoviruses are rod-shaped particles which contain DNA. Most viruses are enclosed in a protein coat to make up a virus inclusion body. Alkaline condition of insect\'s midgut dissolves the protein covering and the viral particles are released from the inclusion body. These particles fuse with the midgut epithelial cells, multiply rapidly and eventually kill the host. But, viral pesticides are more expensive than chemical agents. Furthermore, many baculoviruses are host specific. Therefore they cannot be used to control several different pests. The action of baculoviruses on insect larvae is too slow to satisfy farmers. These viral preparations are not stable under the ultraviolet rays of the sun. Efforts are being made to encapsulate baculoviruses with UV protectants to ensure a longer field-life.
First well-documented introduction of baculovirus into the environment which resulted in effective suppression of a pest occurred accidentally before the World War II. Along with a parasitoid imported to Canada to suppress spruce sawfly
NPVs and GVs are used as pesticides but the group based on nucleopolyhedrosis viruses is much larger. The first viral insecticide Elcar™ was introduced by Sandoz Inc. in 1975Elcar™ was a preparation of
The well-known success of employing baculovirus as a biopesticide is the case of
Protozoan pathogens naturally infect a wide range of insect hosts. Although these pathogens can kill their insect hosts, many are more important for their chronic, debilitating effects. One important and common consequence of protozoan infection is a reduction in the number of offspring produced by infected insects. Although protozoan pathogens play a significant role in the natural limitation of insect populations, few appear to be suited for development as insecticides.
As an other example, the Microsporidia include species promising for biological control. Microsporidian infections in insects are thought to be common and responsible for naturally occurring low to moderate insect mortality. But these are indeed slow acting organisms, taking days or weeks to make harm their host. Frequently they reduce host reproduction or feeding rather than killing the pest outright. Microsporidia often infect a wide range of insects. Some microsporidia are being investigated as microbial insecticides, and at least one is available commercially, but the technology is new and work is needed to perfect the use of these organisms [12]
To be accurate, nematodes are not microbial agents. Instead, they are multicellular roundworms. Nematodes used in insecticidal products are, however, nearly microscopic in size, and they are used much like the truly microbial products discussed previously. Nematodes are simple roundworms. Colorless, unsegmented, and lacking appendages, nematodes may be free-living, predaceous, or parasitic. Many of the parasitic species cause important diseases of plants, animals, and humans. Other species are beneficial in attacking insect pests, mostly sterilizing or otherwise debilitating their hosts. A very few cause insect death but these species tend to be difficult (e.g., tetradomatids) or expensive (e.g. mermithids) to mass produce, have narrow host specificity against pests of minor economic importance, possess modest virulence (e.g., sphaeruliids) or are otherwise poorly suited to exploit for pest control purposes. The only insect-parasitic nematodes possessing an optimal balance of biological control attributes are entomopathogenic or insecticidal nematodes in the genera
The entomogenous nematodes
The infectious stage of these nematodes is the third juvenile stage often referred to as the J3 stage or the "dauer" larvae. Nematodes in this stage survive without feeding in moist soil and similar habitats, sometimes for extended periods.
Bacterial biopesticides are the most common and cheaper form of microbial pesticides. As an insecticide they are generally specific to individual species of moths and butterflies, as well as species of beetles, flies and mosquitoes. To be effective they must come into contact with the target pest, and may require ingestion to be effective. Bacteria in biological pesticides survive longer in the open than previously believed. Bacterial pathogens used for insect control are spore-forming, rod-shaped bacteria in the genus
First of all,
Bacterial insecticides must be eaten to be effective; they are not contact poisons. Insecticidal products comprised of a single
The
There are different strains of
Their study has shown that Bt spores can survive both on the ground and in animals. What’s more, wind, rain and animals can carry them to neighbouring areas. In the splashing rain drops they can even “hop” from the ground up onto leaves - another means of transport. Bt bacteria are also known to be able to easily transfer their toxicity genes to other bacteria in the application area.
When the bacteria were sprayed on cabbage plants, and they were found to have killed all the cabbage white butterfly larvae. In addition, though, the field study revealed that the bacteria are able to survive for a considerable time. After spraying, by far the majority of the spores were found to be present in the upper two centimetres of the soil,
In the present era of transgenic technology, insecticidal toxins of
Different domains involved in the toxicity of
Scientists Per Damgaard and Jørgen Eilenberg at the Royal Agricultural University in Denmark, have also observed examples of spores germinating in living but weakened flies. The flies were already suffering from a severe fungal infection of the lower abdomen, and it was exactly there that the spores germinated. They showed that, the bacterial spores germinate well in dead insects, as the two scientists confirmed by feeding spore and toxin-treated food to larvae of the large cabbage white butterfly.
Under good growth conditions a spore can produce up to a thousand million new spores in a single insect larva.
“There are no previous examples of the spores reproducing in living organisms, although they appear to be able to do so in dead flies. The advantage for the bacterium is that the spores can be spread when the fly moves around,” continue Bjarne Munk Hansen and Jens Chr. Pedersen.
Cry 1A(b) | \n\t\t\tStriped stem borer and leaf folder | \n\t\t\tFujimoto et al. (1993) | \n\t\t
Cry 1A(b) | \n\t\t\tYellow stem borer and striped stem borer | \n\t\t\tWunn et al. (1996) | \n\t\t
Cry 1A(b) | \n\t\t\tYellow stem borer and striped stem borer | \n\t\t\tGhareyazie et al. (1997) | \n\t\t
Cry 1A(b) | \n\t\t\tYellow stem borer | \n\t\t\tDatta et al. (2002) | \n\t\t
Cry 1A(b) | \n\t\t\tYellow stem borer | \n\t\t\tAlam et al. (1999) | \n\t\t
Cry 1A(b)/ Cry 1A(c) | \n\t\t\tLeaffolder and yellow stem borer | \n\t\t\tTu et al. (2000) | \n\t\t
Cry 1A(b)/ Cry 1A(c) | \n\t\t\tYellow stem borer | \n\t\t\tRamesh et al. (2004) | \n\t\t
Cry 1A(c) | \n\t\t\tYellow stem borer | \n\t\t\tNayak et al. (1997) | \n\t\t
Cry 1A(c) | \n\t\t\tYellow stem borer | \n\t\t\tKhanna and Raina (2002) | \n\t\t
Cry 1A(c) | \n\t\t\tStriped stem borer | \n\t\t\tLiu et al. (2002) | \n\t\t
Cry 2A | \n\t\t\tLeaffolder and yellow stem borer | \n\t\t\tMaqbool et al. (1998) | \n\t\t
Cry 2A/ Cry 1A(c) | \n\t\t\tLeaffolder and yellow stem borer | \n\t\t\tMaqbool et al. (2001) | \n\t\t
Cry 1Ie | \n\t\t\tCorn borer | \n\t\t\tLiu et al., 2004 | \n\t\t
Successful examples to show
Insects can be infected with many species of bacteria but those belonging to the genus Bacillus, as alreadily mentioned, are most widely used as pesticides.
Life cycles of biopesticides, bacteria or nematods, to pest larvas. 1- enter the gut or respiratory system through body openings (mouth, anus, and breathing pores. 2- actively penetrate the gut wall, enter the body cavity, and release bacteria. 3- As it multiplies, host dies of septicemia, Adapted from Woodring,1988
The first developed
In most countries of the world, products are available for control of caterpillars (var.
Characterization of the steps require for formation of pores in cell membranes
Also, a relatively new mechanism of action of Cry toxins have been proposed which involves the activation of Mg2+-dependent signal cascade pathway that is triggered by the interaction of the monomeric 3-domain Cry toxin with the primary receptor, the cadherin protein BT-R1 [26 ]. The triggering of the Mg2+-dependent pathway has a knock-on effect and initiates a series of cytological events that include membrane blebbing, appearance of nuclear ghosts, and cell swelling followed by cell lysis. The Mg2+-dependent signal cascade pathway activation by Cry toxins have been shown to be analogous to similar effect imposed by other pore forming toxins on their host cells when they are applied at subnanomolar concentration [37, 38]
Though the two mechanisms of action seem to differ, with series of downstream events following on from toxin binding to receptors on target cell membranes, there is a degree of commonality in that initially the crystals have to be solubilised in vivo or in vitro, and activated by proteases before and/or after binding to receptors such as cadherin [39, 40]
Model of the mode of action of Cry1A toxins.
One approach, to reduce destruction of crops by phytophagous arthropod pests, is to genetically modify plants to express genes encoding insecticidal toxins. The adoption of genetically modified (GM) crops has increased dramatically in the last 11 years. Genetically modified (GM) plants possess a gene or genes that have been transferred from a different species.
The production of transgenic plants that express insecticidal δ-endotoxins derived from the soil bacterium
Corn | \n\t\t\t\n\t\t\t\t | \n\t\t\tEuropean corn borer | \n\t\t\tKoziel et al. (1993) | \n\t\t
Soybean | \n\t\t\t\n\t\t\t\t | \n\t\t\tBollworm and Bud worm | \n\t\t\tStewart (1996) | \n\t\t
Tobacco | \n\t\t\t\n\t\t\t\t | \n\t\t\tCotton bollworm | \n\t\t\tDe Cosa et al. (2001) | \n\t\t
Sugar cane | \n\t\t\t\n\t\t\t\t | \n\t\t\tStem borer | \n\t\t\tArencibia et al. (1997) | \n\t\t
Potato | \n\t\t\t\n\t\t\t\t | \n\t\t\t\n\t\t\t\t | \n\t\t\tDouches et al. (1998) | \n\t\t
Alfalfa | \n\t\t\t\n\t\t\t\t | \n\t\t\tLeaf worm | \n\t\t\tStrizhov et al. (1996) | \n\t\t
Tomato | \n\t\t\tB. thuringiensis (k) | \n\t\t\tTobacco hornworm, tomato pink worm and tomato fruit worm | \n\t\t\tDellannay et al. (1989) | \n\t\t
Brassica | \n\t\t\t\n\t\t\t\t | \n\t\t\tPod borer | \n\t\t\tStewart (1996) | \n\t\t
Cotton | \n\t\t\t\n\t\t\t\t | \n\t\t\tLepidoptera | \n\t\t\tStewart(2001), Chitkowski et al. (2003) | \n\t\t
\n\t\t\t | \n\t\t\t\t | \n\t\t\tPink Bollworm | \n\t\t\tTabashnik et al. (2002) | \n\t\t
Development of some other
Entomopathogenic bacteria, namely
The first reported
It has terminally located sphearical spores. One of the phenotypic characthers examined was patogenicity of some of them to mosquito larvae. A pro-toxin produced during sporulation as in the case of BT, causes fatal cellular alterations when ingested by larvae of some dipteran species. This bacterium has been used to control
Abbott Laboratories has recently formulated a commercial product (Vectolex) of
According to one the old view
The growth of
Endospore formation is a trait found in several microorganisms, which can provide positive benefits to agriculture and varying affects in humans as well. Species of
During their growth cycle, strains of
Because
The bulk of toxicity in Bs comes from the second toxin which is produced at the time of sporulation and it accumulates in the sporangium as a parasporal body, parasporal crystal packed with bacterial spores, in much the same way as that found in
Sporangia of
The two major protein subunits, the 42 and 51 kDa, are both required for full activity and maximum toxicity if they are present in equimolar amounts, suggesting a ‘binary toxin’ mode of action. Studies on the mode of action of Bin toxin suggested that BinB is responsible for the initial binding to the surface of midgut epithelial cells and that BinA confers toxicity. It was also reported that the BinA compound alone can confer toxicity at high doses These two protein subunits are homologous, with 25% identity and four conserved regions between their sequences. P51 is the primary component of binding to the
The mode of action of
After ingestion of the spore-crystal complex by mosquito larvae, the protein crystal matrix quickly dissolves in the lumen of the anterior stomach through the combined action of midgut proteinases and the high pH
Differences in susceptibility between mosquito species seem to differences at the cellular level.The binding of the crystal proteins, P42 and P51 depend on each other. In addition, the internalization of toxin only seems to occur when both components are present. The hyphothesis that a single receptor is involved in the toxin binding was confirmed by in vitro binding assays using radio-labeled activated crystal toxin and midgut brush-border membrane fractions isolated from susceptible mosquito larva It is assumed that the P42 component is the toxic moiety and the P51 is the binding component, the
Toxicity
Action mechanism of VectoLexBC, a Biological Larvicide of
In initial experiments, activity of the protoxin was demonstrated against larvae of the mosquitoe,
Though
The development of a larvicide for use in public health programmes demands selectivity. It should be active against the target species without affecting humans and other non-target populations. The development of a biological larvicide is a process similar to that of the chemical insecticides in that it aims to identify the ideal concentration and form of administration in the field. Formulation is the process used to convert a technical slurry or powder containing the active ingredient produced by the bacterium into a useful and use larvicide compatible with existing application systems. It should also ensure biological stability of the active ingredient and must have an adequate shelf life. It should be easily produced and administered, conveniently stored, and economic [51,52].
It was recently found that the decomposition of organic matter present in aquatic bodies by bacteria lead to the evolution of certain volatile compounds, which attract and/or stimulate gravid female mosquitoes to lay eggs. This finding is a clear indication that bacteria are in great association with mosquito species.
The risk of emergence of resistance should be considered when designing application strategies.
One mechanism of resistance is the reduced binding of the toxin to the midgut receptor sites. As genes for production of insecticidal compounds are added to crop plants, developpers devise methods of preventing or managing insecticide resıstance in target pests. The mechanism of resistance to
Despite the reports of the resistance, the future of
Development of mosquitoe larval resistance against the toxin of commercial microbial larvicide
Depending on the formulation and environmental conditions,
On the other hand, there still exist some resistance or there are some other factors effecting the toxin in the application medium. The effects of aquatic bacterial proteases have been determined only in one study yet. In that study, about 500 bacterial isolates have been obtained from different aquatic mosquito habitats in Turkiye, and then the B. s. larvacidal toxin proteins have been exposed to these extracellular proteases of these bacterial isolates to establish the preliminary screening of the possible effect of these proteases on the B.s. binary toxin proteins. In this study, it was found that, there are also the effects of the environmental microorganisms specifically bacteria due to their extracellular proteases released in the area naturally, so that the B.s. toxin effectiveness in controlling the mosquitoes, especially
The increasing of biological control due to both ecological beneficiancies including the human health as part of world ecology, has been renewed.
The demand for bio-pesticides is rising steadily in all parts of the world. When used in Integrated Pest Management systems, biopesticides\' efficacy can be equal to or better than conventional products, especially for crops like fruits, vegetables, nuts and flowers. By combining performance and safety, biopesticides perform efficaciously while providing the flexibility of minimum application restrictions, superior residue and resistance management potential, and human and environmental safety benefits.
In the study in which the sensitivity of the Bs crystal binary toxin to extracellular proteaese of the aquatic microorganisms were detected, it was shown that there are also the effects of the environmental microorganisms due to their extracellular proteases released in the toxin application area naturally. So that, the Bs toxin effectiveness in the controlling the mosquitoes, especially
In the future other studies can be done as well to detect the type and charactheristics of the effective proteases released into the Bs toxin application areas, so that the preventive manıpulatıons of the Bs toxin protein or some other genetic derivations of the toxin protein may well b eestablished, so that the specific proteaeses would not be able to effct the toxin, while the toxin still can kill the mosquito spp. It is very likely that in future their role will be more significant in agriculture and forestry. Biopesticides clearly have a potential role to play in development of future integrated pest management strategies Hopefully, more rational approach will be gradually adopted towards biopesticides in the near future and short-term profits from chemical pesticides will not determine the fate of biopesticides [62].
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Elements of sustainable design are integral to vernacular architecture that have evolved over time using local materials and technology emerging from ambient natural and cultural environment creating optimum relationships between people and their place. This chapter aims to redefine what identity is as a concept and the impact of globalization on contemporary architecture especially on regions with rich heritage and unique culture as the Arab World. To accomplish this, the chapter examines the emergence of “local identity” as a reaction to the globalization of cultural values, uniform architectural styles, and stereotype patterns through discussing sustainability as a motivation for identity in culture and architecture. The research methodology is based on conducting a qualitative analysis of literature review to the main concepts discussed in this chapter such as: identity, culture, vernacular architecture, and sustainability. 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It is increasingly acknowledged that conventional sewer‐based sanitation cannot be the only solution for expanding urban areas. There are other objective reasons apart from the lack of capital. The lack of stable energy supplies, of spare parts and of human resources for reliable operation, and the increasing water scarcity are factors that seriously limit the expansion of centralised systems. This chapter argues that a new paradigm for urban sanitation is possible, if the heterogeneity within developing cities is reflected in the implementation of different sanitation systems, adapted to each urban context and integrated under one institutional roof. This new paradigm entails: (1) innovative management arrangements; (2) increased participation and the integration of individual, community and private sector initiatives; (3) thinking at scale to open new opportunities; (4) improved analysis of the situation and awareness raising. 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This change influences one another at various temporal and spatial scales; however, improper land uses are the primary causal factor on climate change. It studies relevant literature and Nepal’s case to assess the relationship between land use and climate change. Similarly focuses on how land-use impacts climate change and vice versa. In recent centuries land-use change significant effects on ecological variables and climate change. Likewise, understanding the research on both topics will help decision-makers and conservation planners manage land and climate.",book:{id:"10754",slug:"the-nature-causes-effects-and-mitigation-of-climate-change-on-the-environment",title:"The Nature, Causes, Effects and Mitigation of Climate Change on the Environment",fullTitle:"The Nature, Causes, Effects and Mitigation of Climate Change on the Environment"},signatures:"Pawan Thapa",authors:[{id:"349566",title:"M.Sc.",name:"Pawan",middleName:null,surname:"Thapa",slug:"pawan-thapa",fullName:"Pawan Thapa"}]},{id:"50282",title:"Relation Between Land Use and Transportation Planning in the Scope of Smart Growth Strategies: Case Study of Denizli, Turkey",slug:"relation-between-land-use-and-transportation-planning-in-the-scope-of-smart-growth-strategies-case-s",totalDownloads:4635,totalCrossrefCites:0,totalDimensionsCites:1,abstract:"In the decision-making process of planning residential areas in developing countries, importance of the commercial areas and need for a sustainable urban transportation infrastructure have generally been ignored based on several sociopolitical reasons. Meanwhile, decision-making periods of location choice and determining areal densities are conducted without quantitative spatial/technical analyses. Those urban matters bring along new planning paradigms like smart growth (SG) and new urbanism. SG is a land use planning paradigm which indicates that traffic problems should be minimized by transit alternatives, effective demand management and providing a balance between land use and transportation planning. This study aims to apply SG strategies to the land use planning process and evaluate the accuracy of land use planning decisions in the perspective of sustainable transportation. In order to reveal the effects of land use planning decisions on the available transportation infrastructure, two scenarios are investigated for 2030. In the first scenario “do nothing” option is considered, while the residential area densities and trip generation rates are regulated based on SG strategies in the second scenario. 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Other positions she has held at the university include Vice-Dean of Master Programs, Vice-Dean of the Degree in Biology and Vice-Dean for Mobility and Enterprise and Engagement at the Faculty of Science (University of Alicante). She received her Bachelor in Biology in 1998 (University of Alicante) and her PhD in 2003 (Biochemistry, University of Alicante). She undertook post-doctoral research at the University of East Anglia (Norwich, U.K. 2004-2005; 2007-2008).\nHer multidisciplinary research focuses on investigating archaea and their potential applications in biotechnology. She has an H-index of 21. She has authored one patent and has published more than 70 indexed papers and around 60 book chapters.\nShe has contributed to more than 150 national and international meetings during the last 15 years. Her research interests include archaea metabolism, enzymes purification and characterization, gene regulation, carotenoids and bioplastics production, antioxidant\ncompounds, waste water treatments, and brines bioremediation.\nRosa María’s other roles include editorial board member for several journals related\nto biochemistry, reviewer for more than 60 journals (biochemistry, molecular biology, biotechnology, chemistry and microbiology) and president of several organizing committees in international meetings related to the N-cycle or respiratory processes.",institutionString:null,institution:{name:"University of Alicante",institutionURL:null,country:{name:"Spain"}}},editorTwo:null,editorThree:null},{id:"15",title:"Chemical Biology",coverUrl:"https://cdn.intechopen.com/series_topics/covers/15.jpg",isOpenForSubmission:!0,editor:{id:"441442",title:"Dr.",name:"Şükrü",middleName:null,surname:"Beydemir",slug:"sukru-beydemir",fullName:"Şükrü Beydemir",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y00003GsUoIQAV/Profile_Picture_1634557147521",biography:"Dr. Şükrü Beydemir obtained a BSc in Chemistry in 1995 from Yüzüncü Yıl University, MSc in Biochemistry in 1998, and PhD in Biochemistry in 2002 from Atatürk University, Turkey. 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Dr. Beydemir is also Rector of Bilecik Şeyh Edebali University, Turkey.",institutionString:null,institution:{name:"Anadolu University",institutionURL:null,country:{name:"Turkey"}}},editorTwo:{id:"13652",title:"Prof.",name:"Deniz",middleName:null,surname:"Ekinci",slug:"deniz-ekinci",fullName:"Deniz Ekinci",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002aYLT1QAO/Profile_Picture_1634557223079",biography:"Dr. Deniz Ekinci obtained a BSc in Chemistry in 2004, MSc in Biochemistry in 2006, and PhD in Biochemistry in 2009 from Atatürk University, Turkey. He studied at Stetson University, USA, in 2007-2008 and at the Max Planck Institute of Molecular Cell Biology and Genetics, Germany, in 2009-2010. Dr. Ekinci currently works as a Full Professor of Biochemistry in the Faculty of Agriculture and is the Head of the Enzyme and Microbial Biotechnology Division, Ondokuz Mayıs University, Turkey. He is a member of the Turkish Biochemical Society, American Chemical Society, and German Genetics society. Dr. Ekinci published around ninety scientific papers, reviews and book chapters, and presented several conferences to scientists. He has received numerous publication awards from several scientific councils. Dr. Ekinci serves as the Editor in Chief of four international books and is involved in the Editorial Board of several international journals.",institutionString:null,institution:{name:"Ondokuz Mayıs University",institutionURL:null,country:{name:"Turkey"}}},editorThree:null},{id:"17",title:"Metabolism",coverUrl:"https://cdn.intechopen.com/series_topics/covers/17.jpg",isOpenForSubmission:!0,editor:{id:"138626",title:"Dr.",name:"Yannis",middleName:null,surname:"Karamanos",slug:"yannis-karamanos",fullName:"Yannis Karamanos",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002g6Jv2QAE/Profile_Picture_1629356660984",biography:"Yannis Karamanos, born in Greece in 1953, completed his pre-graduate studies at the Université Pierre et Marie Curie, Paris, then his Masters and Doctoral degree at the Université de Lille (1983). He was associate professor at the University of Limoges (1987) before becoming full professor of biochemistry at the Université d’Artois (1996). He worked on the structure-function relationships of glycoconjugates and his main project was the investigations on the biological roles of the de-N-glycosylation enzymes (Endo-N-acetyl-β-D-glucosaminidase and peptide-N4-(N-acetyl-β-glucosaminyl) asparagine amidase). From 2002 he contributes to the understanding of the Blood-brain barrier functioning using proteomics approaches. He has published more than 70 papers. His teaching areas are energy metabolism and regulation, integration and organ specialization and metabolic adaptation.",institutionString:null,institution:{name:"Artois University",institutionURL:null,country:{name:"France"}}},editorTwo:null,editorThree:null},{id:"18",title:"Proteomics",coverUrl:"https://cdn.intechopen.com/series_topics/covers/18.jpg",isOpenForSubmission:!0,editor:{id:"200689",title:"Prof.",name:"Paolo",middleName:null,surname:"Iadarola",slug:"paolo-iadarola",fullName:"Paolo Iadarola",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bSCl8QAG/Profile_Picture_1623568118342",biography:"Paolo Iadarola graduated with a degree in Chemistry from the University of Pavia (Italy) in July 1972. He then worked as an Assistant Professor at the Faculty of Science of the same University until 1984. In 1985, Prof. Iadarola became Associate Professor at the Department of Biology and Biotechnologies of the University of Pavia and retired in October 2017. Since then, he has been working as an Adjunct Professor in the same Department at the University of Pavia. His research activity during the first years was primarily focused on the purification and structural characterization of enzymes from animal and plant sources. During this period, Prof. Iadarola familiarized himself with the conventional techniques used in column chromatography, spectrophotometry, manual Edman degradation, and electrophoresis). Since 1995, he has been working on: i) the determination in biological fluids (serum, urine, bronchoalveolar lavage, sputum) of proteolytic activities involved in the degradation processes of connective tissue matrix, and ii) on the identification of biological markers of lung diseases. In this context, he has developed and validated new methodologies (e.g., Capillary Electrophoresis coupled to Laser-Induced Fluorescence, CE-LIF) whose application enabled him to determine both the amounts of biochemical markers (Desmosines) in urine/serum of patients affected by Chronic Obstructive Pulmonary Disease (COPD) and the activity of proteolytic enzymes (Human Neutrophil Elastase, Cathepsin G, Pseudomonas aeruginosa elastase) in sputa of these patients. More recently, Prof. Iadarola was involved in developing techniques such as two-dimensional electrophoresis coupled to liquid chromatography/mass spectrometry (2DE-LC/MS) for the proteomic analysis of biological fluids aimed at the identification of potential biomarkers of different lung diseases. He is the author of about 150 publications (According to Scopus: H-Index: 23; Total citations: 1568- According to WOS: H-Index: 20; Total Citations: 1296) of peer-reviewed international journals. He is a Consultant Reviewer for several journals, including the Journal of Chromatography A, Journal of Chromatography B, Plos ONE, Proteomes, International Journal of Molecular Science, Biotech, Electrophoresis, and others. He is also Associate Editor of Biotech.",institutionString:null,institution:{name:"University of Pavia",institutionURL:null,country:{name:"Italy"}}},editorTwo:{id:"201414",title:"Dr.",name:"Simona",middleName:null,surname:"Viglio",slug:"simona-viglio",fullName:"Simona Viglio",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRKDHQA4/Profile_Picture_1630402531487",biography:"Simona Viglio is an Associate Professor of Biochemistry at the Department of Molecular Medicine at the University of Pavia. She has been working since 1995 on the determination of proteolytic enzymes involved in the degradation process of connective tissue matrix and on the identification of biological markers of lung diseases. She gained considerable experience in developing and validating new methodologies whose applications allowed her to determine both the amount of biomarkers (Desmosine and Isodesmosine) in the urine of patients affected by COPD, and the activity of proteolytic enzymes (HNE, Cathepsin G, Pseudomonas aeruginosa elastase) in the sputa of these patients. Simona Viglio was also involved in research dealing with the supplementation of amino acids in patients with brain injury and chronic heart failure. She is presently engaged in the development of 2-DE and LC-MS techniques for the study of proteomics in biological fluids. The aim of this research is the identification of potential biomarkers of lung diseases. She is an author of about 90 publications (According to Scopus: H-Index: 23; According to WOS: H-Index: 20) on peer-reviewed journals, a member of the “Società Italiana di Biochimica e Biologia Molecolare,“ and a Consultant Reviewer for International Journal of Molecular Science, Journal of Chromatography A, COPD, Plos ONE and Nutritional Neuroscience.",institutionString:null,institution:{name:"University of Pavia",institutionURL:null,country:{name:"Italy"}}},editorThree:null}]},overviewPageOFChapters:{paginationCount:43,paginationItems:[{id:"82374",title:"The Potential of the Purinergic System as a Therapeutic Target of Natural Compounds in Cutaneous Melanoma",doi:"10.5772/intechopen.105457",signatures:"Gilnei Bruno da Silva, Daiane Manica, Marcelo Moreno and Margarete Dulce Bagatini",slug:"the-potential-of-the-purinergic-system-as-a-therapeutic-target-of-natural-compounds-in-cutaneous-mel",totalDownloads:4,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Purinergic System",coverURL:"https://cdn.intechopen.com/books/images_new/10801.jpg",subseries:{id:"17",title:"Metabolism"}}},{id:"82103",title:"The Role of Endoplasmic Reticulum Stress and Its Regulation in the Progression of Neurological and Infectious Diseases",doi:"10.5772/intechopen.105543",signatures:"Mary Dover, Michael Kishek, Miranda Eddins, Naneeta Desar, Ketema Paul and Milan Fiala",slug:"the-role-of-endoplasmic-reticulum-stress-and-its-regulation-in-the-progression-of-neurological-and-i",totalDownloads:5,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Updates on Endoplasmic Reticulum",coverURL:"https://cdn.intechopen.com/books/images_new/11674.jpg",subseries:{id:"14",title:"Cell and Molecular Biology"}}},{id:"82212",title:"Protein Prenylation and Their Applications",doi:"10.5772/intechopen.104700",signatures:"Khemchand R. 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Waisundara",profilePictureURL:"https://mts.intechopen.com/storage/users/194281/images/system/194281.jpg",biography:"Dr. Viduranga Waisundara obtained her Ph.D. in Food Science\nand Technology from the Department of Chemistry, National\nUniversity of Singapore, in 2010. She was a lecturer at Temasek Polytechnic, Singapore from July 2009 to March 2013.\nShe relocated to her motherland of Sri Lanka and spearheaded the Functional Food Product Development Project at the\nNational Institute of Fundamental Studies from April 2013 to\nOctober 2016. She was a senior lecturer on a temporary basis at the Department of\nFood Technology, Faculty of Technology, Rajarata University of Sri Lanka. She is\ncurrently Deputy Principal of the Australian College of Business and Technology –\nKandy Campus, Sri Lanka. She is also the Global Harmonization Initiative (GHI)",institutionString:"Australian College of Business & Technology",institution:null}]},{type:"book",id:"6820",title:"Keratin",subtitle:null,coverURL:"https://cdn.intechopen.com/books/images_new/6820.jpg",slug:"keratin",publishedDate:"December 19th 2018",editedByType:"Edited by",bookSignature:"Miroslav Blumenberg",hash:"6def75cd4b6b5324a02b6dc0359896d0",volumeInSeries:2,fullTitle:"Keratin",editors:[{id:"31610",title:"Dr.",name:"Miroslav",middleName:null,surname:"Blumenberg",slug:"miroslav-blumenberg",fullName:"Miroslav Blumenberg",profilePictureURL:"https://mts.intechopen.com/storage/users/31610/images/system/31610.jpg",biography:"Miroslav Blumenberg, Ph.D., was born in Subotica and received his BSc in Belgrade, Yugoslavia. He completed his Ph.D. at MIT in Organic Chemistry; he followed up his Ph.D. with two postdoctoral study periods at Stanford University. Since 1983, he has been a faculty member of the RO Perelman Department of Dermatology, NYU School of Medicine, where he is codirector of a training grant in cutaneous biology. Dr. Blumenberg’s research is focused on the epidermis, expression of keratin genes, transcription profiling, keratinocyte differentiation, inflammatory diseases and cancers, and most recently the effects of the microbiome on the skin. He has published more than 100 peer-reviewed research articles and graduated numerous Ph.D. and postdoctoral students.",institutionString:null,institution:{name:"New York University Langone Medical Center",institutionURL:null,country:{name:"United States of America"}}}]},{type:"book",id:"7978",title:"Vitamin A",subtitle:null,coverURL:"https://cdn.intechopen.com/books/images_new/7978.jpg",slug:"vitamin-a",publishedDate:"May 15th 2019",editedByType:"Edited by",bookSignature:"Leila Queiroz Zepka, Veridiana Vera de Rosso and Eduardo Jacob-Lopes",hash:"dad04a658ab9e3d851d23705980a688b",volumeInSeries:3,fullTitle:"Vitamin A",editors:[{id:"261969",title:"Dr.",name:"Leila",middleName:null,surname:"Queiroz Zepka",slug:"leila-queiroz-zepka",fullName:"Leila Queiroz Zepka",profilePictureURL:"https://mts.intechopen.com/storage/users/261969/images/system/261969.png",biography:"Prof. Dr. Leila Queiroz Zepka is currently an associate professor in the Department of Food Technology and Science, Federal University of Santa Maria, Brazil. 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Her research interests include microalgal biotechnology with an emphasis on microalgae-based products.",institutionString:"Universidade Federal de Santa Maria",institution:{name:"Universidade Federal de Santa Maria",institutionURL:null,country:{name:"Brazil"}}}]},{type:"book",id:"7953",title:"Bioluminescence",subtitle:"Analytical Applications and Basic Biology",coverURL:"https://cdn.intechopen.com/books/images_new/7953.jpg",slug:"bioluminescence-analytical-applications-and-basic-biology",publishedDate:"September 25th 2019",editedByType:"Edited by",bookSignature:"Hirobumi Suzuki",hash:"3a8efa00b71abea11bf01973dc589979",volumeInSeries:4,fullTitle:"Bioluminescence - Analytical Applications and Basic Biology",editors:[{id:"185746",title:"Dr.",name:"Hirobumi",middleName:null,surname:"Suzuki",slug:"hirobumi-suzuki",fullName:"Hirobumi Suzuki",profilePictureURL:"https://mts.intechopen.com/storage/users/185746/images/system/185746.png",biography:"Dr. Hirobumi Suzuki received his Ph.D. in 1997 from Tokyo Metropolitan University, Japan, where he studied firefly phylogeny and the evolution of mating systems. He is especially interested in the genetic differentiation pattern and speciation process that correlate to the flashing pattern and mating behavior of some fireflies in Japan. He then worked for Olympus Corporation, a Japanese manufacturer of optics and imaging products, where he was involved in the development of luminescence technology and produced a bioluminescence microscope that is currently being used for gene expression analysis in chronobiology, neurobiology, and developmental biology. Dr. Suzuki currently serves as a visiting researcher at Kogakuin University, Japan, and also a vice president of the Japan Firefly Society.",institutionString:"Kogakuin University",institution:null}]}]},openForSubmissionBooks:{},onlineFirstChapters:{},subseriesFiltersForOFChapters:[],publishedBooks:{},subseriesFiltersForPublishedBooks:[],publicationYearFilters:[],authors:{}},subseries:{item:{},onlineFirstChapters:{},publishedBooks:{},testimonialsList:[]},submityourwork:{pteSeriesList:[],lsSeriesList:[],hsSeriesList:[],sshSeriesList:[],subseriesList:[],annualVolumeBook:{},thematicCollection:[],selectedSeries:null,selectedSubseries:null},seriesLanding:{item:null},libraryRecommendation:{success:null,errors:{},institutions:[]},route:{name:"profile.detail",path:"/profiles/304033",hash:"",query:{},params:{id:"304033"},fullPath:"/profiles/304033",meta:{},from:{name:null,path:"/",hash:"",query:{},params:{},fullPath:"/",meta:{}}}},function(){var e;(e=document.currentScript||document.scripts[document.scripts.length-1]).parentNode.removeChild(e)}()