Chapters authored
Common Methods to Understand and Develop Indigenous Probiotics Yeast for Ruminant
Probiotic yeast enhanced the ruminal gut microbial balance by producing intercellular effectors and important metabolites. The impact of yeast addition on animal health is influenced by different interlinked factors including animal genomics, its gut microbiota, and environment. Therefore, all factors should be considered regarding achieving the maximum outputs from animal probiotic yeast. In the situation of a high feeding cost, microbial feed supplements provide a suitable nutritional approach, which allows increased nutrient digestion rate and accordingly improves animal performance. Many yeast products are commercially available, but their efficiency as probiotic dietary addition in a particular breed is mostly questionable. Therefore, identification of ideal probiotic yeast strain is of great interest in this context. Innovative methods in relation to develop new probiotic are mainly focused on the exploring novel microbial strains from indigenous sources. It has been noted that for the identification of best probiotic strain for the host, a linkage between culture-independent and culture-dependent methods is a functional step. In this chapter, we will discuss the mode of action of probiotic yeast on animal lower gut microbiota and identification of ideal probiotic yeast by using advanced molecular methods.
Part of the book: Yeasts in Biotechnology
Probiotic Supplement Improves the Health Status and Lactation Performance in Dairy Animals
Probiotics are essential for the effective growth of beneficial bacteria present in enteric line. They help in the physiological functions of new-born calves that are highly susceptible to a variety of fatal syndromes. The criterion for the selection of strains for the design of probiotic products are based on retaining functional health characteristics. Samples from Nili-Ravi buffaloes were collected, and rumen strains are identified for probiotic product. Microscopic techniques with different biochemical tests and molecular techniques such as BLAST have performed for identification. Following species of Weisella has been identified based on genotypic analysis (16S rRNA) under accession number MK336765 (F2) and MK336779 (F4) in the NCBI GenBanK. The strains sharing some of the specific properties evaluated were identified genetically, and their compatibility and exopolysaccharide production were assayed. All of this will be helpful in the production of multi-stain-probiotic product for the nourishment of dairy calves.
Part of the book: Lactation in Farm Animals