Risk categories
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Kalinin",publishedDate:null,coverURL:"https://cdn.intechopen.com/books/images_new/11782.jpg",keywords:"Variety of Traits, Historical Remarks, Modern Definitions and Descriptions, Personality Disorders, Comorbid Psychopathology, Depression, Anxiety, Obsessions, Delusion, Treatment of Personality Disorders, Phenomenology of Personality Traits, Delusional Symptoms",numberOfDownloads:null,numberOfWosCitations:0,numberOfCrossrefCitations:null,numberOfDimensionsCitations:null,numberOfTotalCitations:null,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"March 9th 2022",dateEndSecondStepPublish:"May 12th 2022",dateEndThirdStepPublish:"July 11th 2022",dateEndFourthStepPublish:"September 29th 2022",dateEndFifthStepPublish:"November 28th 2022",dateConfirmationOfParticipation:null,remainingDaysToSecondStep:"2 months",secondStepPassed:!0,areRegistrationsClosed:!1,currentStepOfPublishingProcess:3,editedByType:null,kuFlag:!1,biosketch:'A researcher with over 300 publications in psychopathology, psychopharmacology, neuropsychiatry, and epileptology, a member of the Russian Society of Psychiatry, and the Russian Society of Epileptology. Dr. Kalinin\'s biography is included in Marquis "Who’s Who in Medicine and Healthcare" (2006-2007); Who’s Who in Science and Engineering 2008-2009"; "Who’s Who in the World" (2010, 2011), and in the Cambridge International Biographical Centre.',coeditorOneBiosketch:null,coeditorTwoBiosketch:null,coeditorThreeBiosketch:null,coeditorFourBiosketch:null,coeditorFiveBiosketch:null,editors:[{id:"31572",title:null,name:"Vladimir V.",middleName:null,surname:"Kalinin",slug:"vladimir-v.-kalinin",fullName:"Vladimir V. Kalinin",profilePictureURL:"https://mts.intechopen.com/storage/users/31572/images/system/31572.png",biography:"Vladimir V. Kalinin was born in1952 into a family of physicians in Orenburg (Russian Federation). He obtained an MD from Moscow State Medical Stomatological University in 1976. In 1976-1977 he completed an internship in Psychiatry. 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The GDP of Lithuania grew slightly in 2010, in contrast to a decrease of 14.7% in 2009. Lithuania’s GDP increased from 1.3% in 2010 to 4.6% in 2011. Annual GDP growth decreased from its highest point of 6.7%, reached in the third quarter, to 4.4% in the last quarter of 2011 [1,2]. Some industries, such as construction; trade, transport and communications; and the industry sectors were most affected by the crisis. In 2010, the gross value added within the construction sector decreased by 43.3%, and in the trade, transport and communications sector – by 16.6%. In 2011, a positive change in the gross value added was observed in all groups of economic activities. The largest growth in the gross value added was observed in enterprises engaging in construction (by 15%) and trade, transport and communication services (7.3%) [1,3]. The construction sector, one of the engines of economic growth in Lithuania over the last decade, is now facing with serious challenges as companies’ closures, rising unemployment, and postponed or even cancelled investments. These events also have changed the clients’ and construction companies’ behaviour. A reduced demand and shortage of orders dramatically increased a competition between companies of the construction sector. This increased pressure to improve quality, productivity and reduce costs, and the need for project strategies and management that can appropriately and effectively manage project risk.
Risk management is one of the nine knowledge areas propagated by the Project Management Institute [4]. Furthermore, risk management in the construction project management context is a comprehensive and systematic way of identifying, analyzing and responding to risks to achieve the project objectives [5,6]. The benefits of the risk management process include identifying and analyzing risks, and improvement of construction project management processes and effective use of resources.
Construction projects can be extremely complex and fraught with uncertainty. Risk and uncertainty can potentially have damaging consequences for the construction projects [7,8]. Therefore nowadays, the risk analysis and management continue to be a major feature of the project management of construction projects in an attempt to deal effectively with uncertainty and unexpected events and to achieve project success.
Construction projects are always unique and risks raise from a number of the different sources [9,10]. Construction projects are inherently complex and dynamic, and involving multiple feedback processes [11,12]. A lot of participants – individuals and organisations are actively involved in the construction project, and they interests may be positively or negatively affected as a result of the project execution or project completion [4]. Different participants with different experience and skills usually have different expectations and interests [13]. This naturally creates problems and confusion for even the most experienced project managers and contractors.
Cost of risk is a concept many construction companies have never thought about despite the fact that it is one of the largest expense items [14]. Risk management helps the key project participants – client, contractor or developer, consultant, and supplier – to meet their commitments and minimize negative impacts on construction project performance in relation to cost, time and quality objectives. Traditionally, practitioners have tended to associate construction project success with these three aspects of time, cost and quality outcomes.
The current economic downturn and challenges in a highly competitive Lithuania’s construction sector require contractors to manage risks by themselves. This paper reports the research that aims to examine the risk analysis and risk management practices in the Lithuanian construction companies.
In today’s post-crisis economy effective risk management is a critical component of any winning management strategy. Risk management is one of the nine knowledge areas propagated by the Project Management Institute (PMI). The PMBOK® Guide recognises nine knowledge areas typical of almost all projects. The nine knowledge areas are [4]:
Project integration management.
Project scope management.
Project time management.
Project cost management.
Project quality management.
Project human resource management.
Project communications management.
Project risk management.
Project procurement management.
Although these knowledge areas are all equally important from a project manager’s point of view, in practice a project manager might determine the key areas which will have the greatest impact on the outcome of the project.
Each PMI knowledge area in itself contains some or all of the project management processes. For example, project risk management includes [4]:
Risk management planning;
Risk identification;
Qualitative risk analysis;
Quantitative risk analysis;
Risk response planning;
Risk monitoring and control.
Risk management is probably the most difficult aspect of project management. A project manager must be able to recognise and identify the root causes of risks and to trace these causes through the project to their consequences. Furthermore, risk management in the construction project management context is a comprehensive and systematic way of identifying, analyzing and responding to risks to achieve the project objectives [5,6]. The use of risk management from the early stages of a project, where major decisions such as choice of alignment and selection of construction methods can be influenced, is essential [15]. The benefits of the risk management process include identifying and analyzing risks, and improvement of construction project management processes and effective use of resources.
The construction industry is heterogeneous and enormously complex. There are several major classifications of construction that differ markedly from one another: housing, non-residential building, heavy, highway, utility, and industrial [16]. Construction projects include new construction, renovation, and demolition for both residential and non-residential projects, as well as public works projects, such as streets, roads, highways, utility plants, bridges, tunnels, and overpasses. The success parameters for any project are in time completion, within specific budget and requisite performance (technical requirement). The main barriers for their achievement are the change in the project environment. The problem multiplies with the size of the project as uncertainties in project outcome increase with size [17,18]. Large construction projects are exposed to uncertain environment because of such factors as planning, design and construction complexity, presence of various interest groups (owner, consultants, contractors, suppliers, etc.), resources (manpower, materials, equipment, and funds) availability, environmental factors, the economic and political environment and statutory regulations.
Construction projects can be unpredictable. Managing risks in construction projects has been recognized as a very important process in order to achieve project objectives in terms of time, cost, quality, safety and environmental sustainability [19]. Project risk management is an iterative process: the process is beneficial when is implemented in a systematic manner throughout the lifecycle of a construction project, from the planning stage to completion.
In the European Union construction is the sector most at risk of accidents, with more than 1300 people being killed in construction accidents every year. Worldwide, construction workers are three times more likely to be killed and twice as likely to be injured as workers in other occupations. The costs of these accidents are immense to the individual, to the employer and to society. They can amount to an appreciable proportion of the contract price [20].
Construction activities in Lithuania provided employment to an estimated 93.7 thousand persons in 2011, while an annual turnover in excess of EUR 1.91 billion [21]. Construction is one of Lithuania’s largest industries. Unfortunately it has also the occupational health and safety problems. More construction workers are killed, injured or suffer ill-health than in any other industry. In 2011, 13 construction workers killed whilst at work, compared to 7 industrial workers and 4 agricultural workers. In comparison with 2010, the number of fatal accidents in construction enterprises increased by more than 2 times, i.e. from 6 to 13 cases has been reported [22].
The risk analysis and management techniques have been described in detail by many authors [23-27]. A typical risk management process includes the following key steps [28]:
Risk identification;
Risk assessment;
Risk mitigation;
Risk monitoring.
Risk identification is the first and perhaps the most important step in the risk management process, as it attempts to identify the source and type of risks. It includes the recognition of potential risk event conditions in the construction project and the clarification of risk responsibilities [29]. Risk identification develops the basis for the next steps: analysis and control of risk management. Corrects risk identification ensures risk management effectiveness. Carbone and Tippett [30] stated that the identification and mitigation of project risks are crucial steps in managing successful projects.
The PMBOK® Guide [4] defines a project risk as “an uncertain event or condition that, if it occurs, has a positive or negative effect on at least one project objective”. There are many possible risks which could lead to the failure of the construction project, and through the project, it is very important what risk factors are acting simultaneously. As stated by Raz et al. [31], too many project risks as undesirable events may cause construction project delays, excessive spending, unsatisfactory project results or even total failure.
Many approaches on risk classification have been suggested in the literature for effective construction project risk management. Tah and Carr [32] categorized risks into two groups in accordance with the nature of the risks, i.e. external and internal risks. Combining the fuzzy logic and a work breakdown structure, the authors grouped risks into six subsets: local, global, economic, physical, political and technological change. According to Wang et al. [33], the classification of the risks depends mainly upon whether the project is local or international. The internal risks are relevant to all projects irrespective of whether they are local or international. International projects tend to be subjected to the external risk such as unawareness of the social conditions, economic and political scenarios, unknown and new procedural formalities, regulatory framework and governing authority, etc.
According the PMBOK® Guide [4], the risks are categorized into such groups: technical, external, organizational, environmental, or project management. Some categories of risk that affect a construction project are similar to risks for other investment projects, whether it is an investment in common stocks or government bonds, and some are specific to construction. The risk identification process would have highlighted risks that may be considered by project management to be more significant and selected for further analysis [34]. Risk identification is an iterative process because new risks may become known as the project progresses through its life cycle and previously-identified risks may drop out [35]. Construction projects carry complex risks for all involved—including owners, consultants, contractors, and suppliers—that can increase when construction takes place near an active facility or congested area. Risks include geological or pollution-related conditions, interference with ongoing operations, construction accidents, as well as design and construction faults that may negatively impact the project both construction and when the project is complete.
Generally two broad categories, namely, qualitative and quantitative analysis are distinguished in literature on risk assessment. A qualitative analysis allows the key risk factors to be identified. Risk factors may be identified through a data-driven (quantitative) methodology or qualitative process such as interviews, brainstorming, and checklists. It is considered as an evaluation process which involves description of each risk and its impacts or the subjective labelling of risk (high/medium/low) in terms of both risk impact and probability of its occurrence [19]. Qualitative risk analysis assesses the impact and likelihood of the identified risks and develops prioritized lists of the risks for further analysis or direct mitigation. Carr and Tah [36] introduced a hierarchical risk breakdown structure (HRBS), and the HRBS represents a formal model for qualitative risk assessment. Quantitative analysis involves more sophisticated techniques and methods to investigate and analyze construction project risks. Quantitative risk analysis attempts to estimate the frequency of risks and the magnitude of their consequences by different methods such as the decision tree analysis, the cost risk analysis, and Monte Carlo simulation [37]. The application of the quantitative risk analysis allows the construction project exposure to be modelled, and quantifies the probability of occurrence of the identified risk factors as well as their potential impact.
Various risk management tools are available, but unfortunately they are not suitable for many industries, organizations and projects [38]. Although today’s organizations appreciate the benefits of managing risks in construction projects, formal risk analysis and management techniques are rarely used due to lack of knowledge and to doubts on the suitability of these techniques for construction projects.
There are four alternative strategies – risk avoidance, risk transfer, risk mitigation, and risk acceptance, for treating risks in a construction project. As stated by Hillson [39], risk mitigation and risk response development is often the weakest part of the risk management process. The proper management of risks requires that they be identified and allocated in a well-defined manner. This can only be achieved if contracting parties comprehend their risk responsibilities, risk event conditions, and risk handling capabilities [40].
Before the crisis (2004-2008), due to a lack of contractors’ responsibilities and control in various steps of a project’s development, the time and quality performance levels of construction projects in the Lithuania were generally inadequate or even poor. In construction projects, many parties are involved such as owner, consultant, contractor, sub-contractor, and supplier. Each party has its own risks. Risk transfer means the shift of risk responsibility to another party either by insurance or by contract. Wang and Chou [29] reported that contractors usually use three methods to transfer risk in construction projects:
through insurance to insurance companies;
through subcontracting to subcontractor;
through modifying the contract terms and conditions to client or other parties.
Construction projects can be managed using various risk management tools and techniques. Ahmed et al. [23] reviewed techniques that can be used for development of risk management tools for engineering projects. Techniques for context establishment, risk identification, risk assessment and treatment were provided. Application of risk management tools depends on the nature of the project, organization’s policy, project management strategy, risk attitude of the project team members, and availability of the re-sources [12]. A risk assessor model (RAM) presented by Jannadi and Almishari [41] was developed to determine risk scores for various construction activities. The model provides an acceptability level for the risks and determines a quantitative justification for the proposed remedy.
Risks and uncertainties, involved in construction projects, cause cost overrun, schedule delay and lack of quality during the progression of the projects and at their end [28,29,42]. As stated by Baloi and Price [43], poor cost performance of construction projects seems to be the norm rather than the exception, and both clients and contractors suffer significant financial losses due to cost overruns.
Oyegoke et al. [44] discusses the problems of managing risk and uncertainty in construction project due to the owner dissatisfaction in project outcome and dynamism within agile construction environment. The authors identified some areas in supply chain processes which are prone to greater risks and uncertainty and propose an agile management principle based on the concept of integration and fragmentation in product development and execution processes respectively.
Many authors have reviewed problems on time performance in construction projects [43,45,46]. Aibinu and Odenyinka [46] investigated and assessed the causes of delays in building projects in Nigeria. The nine factor categories evaluated include: client-, contractor-, quantity surveyor-, architect-, structural engineer-, services engineer-, supplier-, and subcontractor-caused delays, and external factors (i.e. delays not caused by the project participants). Finally, ten overall delay factors were identified, namely: contractors’ financial difficulties, client’ cash flow problems, architects’ incomplete drawings, subcontractors’ slow mobilization, equipment break-down and maintenance problems, suppliers; late delivery of ordered materials, incomplete structural drawings, contractors’ planning and scheduling problems, price escalation, and subcontractors’ financial difficulties. The authors pointed the poor risk management as one of the principal delay factors and concluded that actions and inactions of construction project participants contribute to overall project delays.
According to Baloi and Price [43], the construction contractors highlight that delay in payments is common both in private and public projects, with the public sector being the worse defaulter. Moreover, most types of contracts presume compensation clauses for delay in payments, but clients rarely agree to pay the interests due to the contract. Nasir et al. [47] analysed schedule risks and developed a comprehensive construction schedule risk model is referred to as Evaluating Risk in Construction–Schedule Model (ERIC-S). The ERIC-S model provides decision support to project owners, consultants, and re-searchers as a project delay prediction tool. Similarly, the Cost-Time-Risk diagram (CTR) proposed by Aramvareekul and Seider [48] helps project managers consider project risk issues while monitoring and controlling their project schedule and cost performance in one diagram.
The performance by the project management team highly influences the success of a construction project. Some of the incidental risks associated with poor project management performance are [49]:
Unclear or unattainable project objectives;
Poor scoping;
Poor estimation;
Budget based on incomplete data;
Contractual problems;
Insurance problems;
Delays;
Quality concerns;
Insufficient time for testing.
Many authors have recognized the value of trust within the project business. Lewicki and Bunker [50] emphasize that trust is a critical success element to most business, professional, and employment relationships. Trust is argued to improve the inter-organizational relationships among principal actors in project development, such as owners, contractors, and suppliers [51]. According by Krane et al. [52] trust between project owners and project managers is crucial for project success.
In business relations, as stated by Kaklauskas et al. [53], the global economic crisis brought about distrust of other stakeholders. Trust reinforces the relationships of the critical stakeholder that often determine the success of a project [51,54,55]. Ward and Chapman [56] concluded that stakeholders are a major source of uncertainty in construction projects. Smyth et al. [57] note that trust provides an important resource for creating greater probability and certainty. Wilkinson [58] found that project management companies need to overcome problems in their relationships with other professionals on the project team and with the client. For the success of construction projects, there is a need for alignment of the project owners’ interests and the project management team\'s interests and trust between them.
Construction projects are tendered and executed under different contract systems and payment methods [59]. According by Zaghloul and Hartman [60], there is no possibility to eliminate all the risks associated with a specific project. All that can be done is to regulate the risk allocated to different parties and then to properly manage the risk. Chapman and Ward [61] argue that the contract choice decisions are central to both stakeholder management and the management of risk and uncertainty. The authors proposed an integrated approach based on a balanced incentive and risk sharing (BIARS) approach to contracting as well as a best practice approach to risk management in terms of the whole project life cycle.
Contractors generally aim to make an acceptable range of profit margin. Profit margins in the industry have been low for most contractors on projects in recent years. Correct understanding and allocation of risk helps for contractors to avoid erosion of the profit margin. Ökmen and Öztas [62] proposed a new simulation-based model – the correlated cost risk analysis model (CCRAM) – to analyse the construction costs under uncertainty when the costs and risk-factors are correlated. The CCRAM model captures the correlation between the costs and risk-factors indirectly and qualitatively. Baloi and Price [63] determined the most critical risk factors affecting construction cost performance. The authors stated that global risk factors pose more challenges to contractors, which are less familiar with them. The authors introduced a fuzzy decision framework for a systematic modelling, analysis and management of global risk factors affecting construction cost performance from contractor’s perspective and at a project level. Similarly, Ismail et al. [64] provide a ‘Level-Severity-Probability’ approach to determine the critical risk source and factors. Fuzzy logic is used in the proposed methodology for evaluation of the risk level, severity and probability. As stated by Zeng et al. [65], the application of fuzzy reasoning techniques provides an effective tool to handle the uncertainties and subjectivities arising in the construction project.
The review of the literature revealed a wide range of risk types and sources in construction projects, and that various risk management methods and techniques can be employed in the management of construction projects in order to control potential risks.
The aims of the research were: first, to identify contractors’ opinion on the significance of the construction projects risks; and second, to explore the risk analysis and risk management practices in the Lithuanian construction companies.
The initial survey was distributed during February through March 2008. A second, similar questionnaire was distributed during February through March 2009. A questionnaire containing three sections was developed to facilitate data collection. The first section includes the respondents’ opinion on the risk factor in terms of its probability and impact to overall construction project success. The second section includes the respondents’ opinion on the risk consequences for construction project performance measures as well as the risk assessment and response practices. The third section aims to collect the background information of the respondents, e.g. their age, gender, position, education, work experience and professional background.
The questionnaire of first survey was distributed either personally or via e-mail to 40 members of top and middle management in the construction companies. A sample of 40 practitioners received the questionnaire and 38 valid questionnaires were returned for analysis with a response rate of 95%. The second questionnaire was distributed either personally or via e-mail to 35 members of top and middle management in the construction companies. Of the 35 questionnaires distributed in the second survey, 35 were returned, but 5 were incompletely completed and so were excluded from the data analysis. The response rate was 86%.
In both surveys, the baseline characteristics of the respondents were relatively similar. Of the 38 respondents in the first study, site managers comprise 29%, project managers 26%, other position senior managers 21%, civil engineers 16%, and designing engineers 8%. Of the 30 respondents in the second study, site managers, project managers, and other position senior managers comprise 80%.
The Likert scale was selected to obtain the probability of the risk factors in construction project that are identified in the literature review. A 5-point Likert scale was adopted, where 1 represented “rare”, 2 “occasional”, 3 “somewhat frequent”, 4 “frequent”, and 5 “very frequent”. Likewise, the Likert scale was selected to obtain the impact of the risk factors in construction project that are identified in the literature review. A 5-point Likert scale was adopted, where 1 represented “very low”, 2 “low”, 3 “moderate”, 4 “high”, and 5 “very high”.
The latest survey was carried out in December 2010-January 2011. In a subsequent study, the role of risk factors at a project level was addressed. A questionnaire containing three sections was developed to facilitate data collection. The first section includes the respondents’ opinion on the risk consequences for construction project performance measures as well as the risk assessment and response practices. The second section includes the respondents’ opinion on the risk factor in terms of its probability and impact to overall construction project success. The third section aims to collect the background information of the respondents, e.g. their age, gender, position, education, work experience and professional background.
The third questionnaire was distributed either personally or via e–mail to 23 members of top and middle management in the construction company and 23 valid questionnaires were returned for analysis in time to be included in the analysis (100% overall response rate). Of the 23 respondents in the study, site managers, project managers, and other position senior managers comprise 91%.
In all surveys, the majority of the respondents have more than 15 years’ experience in construction/project management or working knowledge of construction/project management activities. Based on work experience and employment position, it was inferred that the respondents have adequate knowledge of the activities associated with construction project risk. This makes them as reliable and credible sources of information which is crucial to satisfy the research goal. The procedure, findings, and relevant discussion of the analyses are detailed in the following section.
As outlined in Section 2, risk factors on construction projects can be split into two major groups:
Internal risks, which fall within the control of clients, consultants and contractors.
External risks, which include risk elements that are not in the control of key stakeholders.
The potential risk sub-factors were adapted from studies by Chapman and Ward [25], Tah and Carr [32], Perera et al. [40], Pinto et al. [51], Baloi and Price [63], Kartam and Kartam [66], Lahdenperä [67], Majamaa et al. [68], Mbachu and Nkado [69], Mitkus and Trinkūnienė [70], and Yang et al. [71].
In order to illustrate the respondents’ opinions regarding the importance of analysed risk factors, an average was calculated for each factor. Next, the Kendall coefficient of concordance W [72,73] was calculated to test the reliability of the responses, and significance testing was based on the Chi-square distribution at the 1% significance level. The
In both surveys, the respondents agree as regards the external risks impact and probability. The respondents agree as regards the external risks impact, what can be judged by values
Natural forces;
Inflation and interest rate;
Fiscal policy.
In the second survey, the top three important external risks identified are:
Fiscal policy;
Natural forces;
Political controls.
Probability assessment of risks of the external project constrains is reflected in Fig. 1. Impact assessment of risks of the external project constrains is reflected in Fig. 2.
The risk management perceivers are the project participants, and a contractor is any entity which has the power to influence project decision making directly. Related to experience, only 11% of the respondents affirmed that they have experience in risk management. Most of them are project manager and have more than 15 years’ experience; it proofs that the relationship between risk perception and experience of respondents. And even 34% of the respondents affirmed that they have no experience in risk management, while 55% of the respondents affirmed that they do not have enough experience in risk management. And 97% of the respondents answered that risks must be managed at the early stages of the construction project.
Probability assessment of external project risks
Impact assessment of external project risks
In terms of the sources and providers of the data and information required in the risk analysis, the most frequently used technique is experiential or documented knowledge analysis with 92% of the respondents’ agreement in the first survey, and 93% of the respondents’ agreement in the second survey (Fig. 3). And the project documentation reviews, project team brainstorming, and analysis of other information resources are frequently used in the risk assessment.
Comparison between the two surveys in terms of risk analysis showed a decrease in reviews of project documentation, from 63% in the first survey to 47% in the second survey, as well as greater use of experts’ judgement, from 26% in the first survey to 43% in the second survey, and project team brain-storming, from 45% in the first survey to 53% in the second survey, in the risk assessment.
Risk analysis practices in construction projects
Risk response techniques employed for construction projects
In terms of the risk response tools and techniques, the most frequently used tool is performance bonds and warranties with 95% of the respondents’ agreement in the first survey, and 77% of the respondents’ agreement in the second survey (Fig. 4). And the some resource reservation, insurance, and risk transference to another project party are frequently used risk response techniques.
Comparison between the two surveys in terms of risk response tools and techniques showed a decrease of performance bond and warranties, from 95% in the first survey to 77% in the second survey, and resource reservation, from 61% in the first survey to 50% in the latter survey; as well as greater use of risk transference to another party, from 50% in the first survey to 53% in the second survey, and insurance, from 55% in the first survey to 57% in the latter survey, for the risk responses.
In last survey (2010-2011), the respondents agree as regards the project level risks impact, what can be judged by values
Construction risks;
Design risks;
Project management risks.
Overall assessment of risks of the internal project risks is reflected in Fig. 5. Risk priority is utilized during response planning and risk monitoring. It is critical to understand the priority for each risk as it allows the project team to properly understand the relative importance of each risk.
Assessment of project level risk categories
Table 1 shows that the controllable risk sources as identified in the study could be further broken down into seven sub-categories: design risks, external risks, environmental risks, organizational risks, project management risks, right of way risks, and construction risks which fall within the control of the project team.
Categories | Likelihood 1 (rare)-5 (very frequent) | Impact 1 (very low)-5 (very high) | |
Design risks | |||
D1 | Design errors and omissions | 4 | 5 |
D2 | Design process takes longer than anticipated | 3 | 4 |
D3 | Stakeholders request late changes | 3 | 3 |
D4 | Failure to carry out the works in accordance with the contract | 3 | 3 |
External risks | |||
Ex1 | New stakeholders emerge and request changes | 2 | 4 |
Ex2 | Public objections | 1 | 3 |
Ex3 | Laws and local standards change | 1 | 3 |
Ex4 | Tax change | 1 | 4 |
Environmental risks | |||
En1 | Environmental analysis incomplete | 2 | 4 |
En2 | New alternatives required to avoid, mitigate or minimize environmental impact | 2 | 4 |
Organizational risks | |||
O1 | Inexperienced workforce and staff turnover | 3 | 3 |
O2 | Delayed deliveries | 3 | 3 |
O3 | Lack of protection on a construction site | 2 | 4 |
Project management risks | |||
PM1 | Failure to comply with contractual quality requirements | 3 | 4 |
PM2 | Scheduling errors, contractor delays | 4 | 4 |
PM3 | Project team conflicts | 3 | 3 |
Right of way risks | |||
R1 | Expired temporary construction permits | 1 | 4 |
R2 | Contradictions in the construction documents | 2 | 3 |
Construction risks | |||
C1 | Construction cost overruns | 4 | 4 |
C2 | Technology changes | 2 | 4 |
Risk categories
Very high | |||||||
High | PM2, C1 | D1 | |||||
Moderate | D3, D4, O1, O2, PM3 | D2, PM1 | |||||
Low | R2 | Ex1, En1, En2, O3, C2 | |||||
Very low | Ex2, Ex3 | Ex4, R1 | |||||
Rare | Occasional | Somewhat frequent | Frequent | Very frequent | |||
Risk matrix
Once the risks and probabilities are determined, the risk score can be calculated. Risk score is detailed in Table 1. The probability and impact matrix (Fig. 6) illustrates a risk rating assignment for individual risk factors in the identified risks categories. The risk matrix shows the combination of impact and probability that in turn yield a risk priority (shown by the red, yellow, and green colour). Qualitative risk analysis can lead to further analysis in quantitative risk analysis or directly to risk response planning.
Twenty risk factors were established to be significant under the internal risks categories. Under the design risk category, design errors/omissions and design process delays were the most frequently mentioned risk factors attributed to the contractors. Under the project management risk category, scheduling errors and failure to comply with contractual quality requirements were the most frequently mentioned risk factors. Under the construction risk category, construction cost overruns and technology changes were the most frequently mentioned risk factors attributed to the contractors. Respondents believed that these risk events are responsible for poor quality of work, delays and associated losses. Risks with high impact and high probability, such as D1 (design errors and omissions), C1 (construction cost overruns), and PM2 (scheduling errors, contractor delays) are required further analysis, including quantification, and aggressive risk management.
An effective risk management process encourages the construction company to identify and quantify risks and to consider risk containment and risk reduction policies. Construction companies that manage risk effectively and efficiently enjoy financial savings, and greater productivity, improved success rates of new projects and better decision making.
Risk management in the construction project management context is a comprehensive and systematic way of identifying, analyzing and responding to risks to achieve the project objectives. The research results show that the Lithuanian construction company significantly differ from the construction companies in foreign countries in the adoption of risk management practices. To management the risk effectively and efficiently, the contractor must understand risk responsibilities, risk event conditions, risk preference, and risk management capabilities.
The lack of experience makes it very difficult to change Lithuanian contractors’ attitude towards risk management. Nevertheless, the construction companies need to include risk as an integral part of their project management. In our view, the use of risk management in the Lithuanian construction companies is low to moderate, with little differences between the types, sizes and risk tolerance of the organizations, and experience and risk tolerance of the individual respondents.
Qualitative methods of risk assessment are used in construction companies most frequently, ahead of quantitative methods. In construction project risk management, risks may be compared by placing them on a matrix of risk impact against a probability. Mitigation options are then derived from predefined limits to ensure the risk tolerance and appetite of the construction company.
The risk management framework for construction projects can be improved by combining qualitative and quantitative methodologies to risk analysis.
Food matrices having water activities above 0.9 and wet food processing environments are wonderlands for microorganism multiplication and biofilm development. Biofilms are considered of great concern in regard to functioning of mechanical parts that may be blocked, to energy consumption, which becomes higher when heat transfer decreases, and to corrosion as corrosion rate of surfaces increases underneath biofilms (corrosion grows 10–1000 times faster causing loss of material and increasing porosity) but their presence in food and food processing environments is also a serious public health risk due to problems associated with foodborne illnesses and food spoilage [1].
\nThe biofilms that are threatening the safety of food products are produced by some pathogenic bacteria such as
Biofilms are formed on all types of surfaces existing in food plants ranging from plastic, glass, metal, cement, to wood and food products [4]. Usually, biofilms form a monolayer or more often multilayers, in which bacteria may undergo a significant change in physiology with an increased tolerance to environmental stresses [5].
\n\n
\n
Flagella, pili, and membrane proteins are also used by
In general terms, different
\n
\n
\n
Within the biofilm,
Four mechanisms based on the flagellar motility of
It has been showed that, in its planktonic form,
Over time, beside the conditions that favor the biofilm formation in food processing plants, the genetic background of biofilm forming microorganisms was also intensively studied. At each step of biofilm development and dispersal, there is a specific genetic signal control.
\nThe
In the biofilm formation, the attachment step is a prerequisite in which flagella and type I pili-mediated motilities are critical for the initial interaction between the cells and surface.
\nIn order to find out the roles of the genes and regulatory pathway controlling the biofilm formation, researchers applied one or two genome-wide approaches, like transposon insertion mutagenesis or/and transcriptome analyses. With a transposon mutagenesis library, it was possible to identify 70
Gene/KEGG/protein encoded | \nGene function | \nRole | \nBacterium | \nRef. | \n
---|---|---|---|---|
\n | \nMotility Flagella bio-synthesis | \nCell adhesion and bacterial attachment | \n\n | \n[23–25] | \n
\n | \n||||
\n | \nEnable the polymerization of the flagellin monomers; flagellar capping protein | \n[148] | \n||
\n | \nFlagellar motor rotation | \n|||
\n | \nDNA-binding transcription factor activity | \nPositive regulation of single species biofilm formation | \n\n | \n|
\n | \nEnable bacteria to colonize the host epithelium | \nCell adhesion | \n\n | \n[30, 31] | \n
\n | \nMotility bacterial-type flagellum assembly | \n|||
\n | \nFimbrial bio-synthesis | \n|||
\n | \nRepresses fimbria genes | \nSingle-species biofilm | \n||
\n | \nAcetylglucosaminyl transferase activity, cell adhesion | \nInvolved in the polymerization of a biofilm adhesin polysaccharide | \n\n | \n[149] | \n
\n | \nInvolved in gluconeogenesis pathway | \nRole in adherence | \n[150] | \n|
\n | \nMediates binding to human platelets | \nPlays a positive role in biofilm formation | \n[151] | \n|
\n | \nRegulatory role in sporulation | \nSingle-species surface biofilm formation | \n\n | \n[152] [153] | \n
\n | \nTransition to growth phase; flagellum formation | \nBiofilm formation | \n[154, 155] | \n|
\n | \nControls the rotational direction of flagella | \nMotility, cell adhesion | \n\n | \n[156] | \n
\n | \nPromotes the attachment to the surface | \n[157] | \n||
\n | ||||
\n | \nCatalyzes the first step in the D-alanylation of lipoteichoic acid (LTA) | \nCell wall biogenesis | \n\n | \n[24] | \n
\n | \nCarrier protein involved in the D-alanylation of LTA | \n|||
\n | \nInvolved in the transport of activated D-alanine through the membrane | \n\n | \n||
\n \n | \nCell adhesion | \nMediates interactions with components of the extracellular matrix to promote bacterial adhesion | \n\n | \n[158] | \n
\n | \nStress response, response to copper ion | \nRegulation of biofilm formation. May repress cell–cell interaction and cell surface interaction | \n\n | \n[159] | \n
\n | \nStress response to hydrogen peroxide and to DNA damage | \nSingle-species biofilm formation; enhanced flagellar motility | \n\n | \n[160] | \n
\n | \nDNA-binding transcription activator activity | \nThe master regulator for adhesive curli fimbriae expression | \n[161] | \n|
\n | \nDNA-binding transcription factor activity | \nActivates transcription of | \n[162] | \n|
\n | \nNegatively regulates transcription of the | \nDNA-binding protein master regulator of biofilm formation | \n\n | \n[163, 164] | \n
\n | \nProduction of exopolysaccharide | \nBiofilm maintenance | \n[165] | \n|
\n | \n[166] | \n|||
\n | \nRegulatory role. Induces genes involved in biofilm formation | \nDirecting the early stages of colony development | \n||
\n | \nCatalyzes the interconversion between glucose-6-phosphate and alpha-glucose-1-phosphate | \nExopolysaccharide synthesis | \n[167] | \n|
\n | \nBiofilm production under low-nutrient concentrations | \n\n | \n | \n[156] | \n
\n | \nIdentical protein binding | \nMajor component of the biofilm extracellular matrix | \n\n | \n[168] | \n
\n | \nImportant for proper anchoring and polymerization of TasA fibers at the cell surface | \nEssential for biofilm formation | \n\n | \n[169] | \n
\n | \nCleavage of the signal sequence of TasA and TapA | \n\n | \n||
\n | \nConfers a specific microstructure to the biofilm surface | \nConfers hydrophobicity to the biofilm | \n\n | \n[170–171] | \n
\n | \nSynthesis of colanic acid | \nInvolved in the pathway slime polysaccharide biosynthesis | \n\n | \n[172] | \n
\n | \n\n | \n[173] | \n||
\n | \nRegulation of biofilm formation | \nIn the glucose presences, cells showed increased biofilm formation | \n\n | \n[33] | \n
\n | \nMotility-quorum sensing cell proliferation | \nBiofilm architecture | \n[172] | \n|
\n | \nEfflux transmembrane transporter activity | \nRepresses biofilm formation and motility | \n[31] | \n|
\n | \nControls cell motility, size, aggregation, and production of extracellular DNA and extracellular polysaccharides | \nBiofilm dispersal | \n\n | \n[174] | \n
\n | \nSpecific DNA-binding protein | \nMatrix density Cellulose production | \n\n | \n[175–177] | \n
\n | \nLarge surface proteins family | \nBacterial adhesion Biofilm maturation | \n||
\n | \nCell surface-associated protein implicated in bacterial attachment | \nAggregation of unicellular organisms; cell adhesion | \n\n | \n[178] | \n
\n | \nExport of PNAG across the cell membrane | \n\n | \n[149] | \n|
\n \n | \nEnzymes that catalyze the first step in the acetogenesis from pyruvate | \nOrganic free radical synthesis | \n[29] | \n|
\n | \nGlobal regulator of a few genes with important roles in biofilm development | \nBiofilm formation process in a cell density-dependent manner | \n\n | \n[179] | \n
\n | \nInvolved in proteolytic processing | \nQuorum Sensing | \n\n | \n[180] | \n
lmo0048/Putative AgrB-like protein | \nInvolved in proteolytic processing | \n\n \n | \n||
\n | \nHistidine kinase activity | \n\n | \n[181] | \n|
\n Accessory gene regulator | \nA response regulator | \n[182] [183] | \n||
\n | \nProteolytic processing of | \n\n | \n[184] | \n|
luxS/lmo1288/S-ribosyl-homo-cysteine lyase | \nCatalysis of precursor molecules of AI-2 | \n\n | \n[48] [49] | \n|
\n | \nPhospho-relay sensor kinase activity | \n\n | \n
List of genes with significant role in biofilm formation within pathogenic microorganisms (UniprotKB database).
Extracellular and surface proteins such as internalin A and BapL, respectively, have been found to be involved in the initial bacterial adhesion in
\n
In the case of
Moreover,
Microcolonies are formed by cell proliferation, and many genes involved in cell division, cell wall biogenesis, virulence and motility, stress response, and transcriptional regulation factors are expressed.
\n\nTable 1 shows a selection of the genes that are expressed in all the steps of biofilm formation or are upregulated under influence of different biotic or abiotic factors. It was reported that the ∆
The mature biofilm evolves from microcolonies and this development is associated with EPS production. The biofilm matrix of
Furthermore, the structural proteins encoded by
An important polysaccharide identified in the matrix biofilm of many pathogenic bacteria is the colanic acid, which plays an important physiological role for bacteria living in biofilm. This EPS is synthesized by specific enzymes encoded by
Consequently, the transition from the planktonic state to the biofilm state is critical and it is subjected to a strict gene regulation, essential for matrix synthesis, cell aggregation, and cell signaling.
\nNevertheless, bacteria of multiple genetic backgrounds communicate by regulating their relationship of cooperativeness through a mechanism called quorum sensing (QS) in which the bacterial cells are having social interactions with each other through small diffusible signal molecules called autoinducers, thus contributing to the biofilm development [10].
\nQuorum sensing process described in the 1970s is involved in the control of various gene expressions through chemical signaling molecules that are synthesized in response to cell population density [39]. When bacteria start to sense their critical biomass, they answer by activating or repressing genes from 10% of bacteria genome [40]. The system has been described for both Gram-negative and Gram-positive bacteria.
\nAmong QS, other two important regulators are known to control biofilm shape and structure: cyclic diguanosine-5′-monophosphate (c-di-GMP) and small RNAs. For example,
The c-di-GMP involvement in
Gram-positive bacteria such as
The accessory gene regulator of
For
The
Since biofilms act as a barrier that protects the embedded cells against cleaning and disinfecting agents [51], the control of biofilm is an issue that is currently addressed to find effective solutions that can prevent biofilm formation or eliminate the already formed one. Biocontrol of biofilms by using bacteriocins, disruptive enzymes, essential oils, or bacteriophages is gaining importance, as well as using nanoemulsions and nanoparticles. These new methods are promising strategies with remarkable results in the fight against biofilms.
\nBacteriocins are antimicrobial peptides ribosomally produced by an extensive range of bacteria to inhibit or kill competing microorganisms in a micro-ecological system [52, 53]. The most studied bacteriocin and the only one allowed presently as food-grade additive is nisin, a lantibiotic with proven effects against many Gram-positive bacteria including foodborne pathogens [54]. This bacteriocin was shown to penetrate the biofilm formed by
Another way to prevent biofilms development is represented by the adsorption of these bioactive compounds on the surfaces that come into contact with foods [59]. In this case, Nisaplin adsorbed to three types of food-contact surfaces commonly encountered in food processing plants, namely stainless steel, polyethylene terephthalate (PET), and rubber, reduced the adhesion ability of food-isolated
A bacteriocin found to markedly inhibit the biofilm formed by
Disruptive enzymes, such as proteases, glycosidases, amylases, cellulases, or DNAses, are considered a green alternative to chemical treatments often used in the fight against biofilms’ formation in food-related environments [2]. Such enzymes do not have toxic effects and are used both alone and as part of the industrial detergents’ composition to improve their cleaning efficacy [64–66].
\nProteases are a class of enzymes that catalyzes the cleavage of proteins’ peptide bonds. Although they are produced by all living organisms, microbial proteolytic enzymes are preferred over animal or plant origin proteases. The most commonly used source of bacterial proteases is represented by those produced by the genus
Polysaccharide-hydrolyzing enzymes were indicated to remove the biofilms formed by
Plant essential oils (EOs) are rich in phytochemical compounds, which are secondary metabolites produced by plants as defense mechanism against pathogens [74]. Regarding microbial inactivation, EOs have been reported to mainly affect the cellular membrane by permeabilization [75]. This leads to the disruption of vital cellular processes, including energy production, membrane transport, and metabolic regulatory functions [76].
\nStudies evaluating the potential of EOs as disinfectants were conducted. Leonard et al. [77] assessed the bioactivity of
Thyme EO has proven antimicrobial properties [80]. In terms of biofilm inhibition capacity, this EO was shown to inhibit significantly the biofilm formed by
Eugenol is a phytochemical compound preponderantly found in aromatic plants [89]. Interestingly, a study showed that this substance was able to inhibit the intracellular signaling pathway called quorum sensing in the case of biofilms formed by methicillin-resistant
Bacteriophages are viruses that infect bacterial cells. They use the genetic machinery of their host cells to replicate, killing bacteria when reaching a sufficiently high number to produce lysis [92]. They are abundantly encountered anywhere host bacteria live [93] and, therefore, their potential is presently harnessed as natural antimicrobial agents to control pathogenic bacteria in food products and food-related environments [94]. One of the bacteriophages’ applications that is intensively explored targets biofilm-forming bacteria that are relevant for food industry, including
Although it is generally thought that biofilms confer resistance to bacteriophages, these bacterial predators developed several mechanisms to destroy bacteria communities. Once they reach the EPS (extracellular polymeric substances) producing host, they start to replicate, resulting in an increased number and, implicitly, in a progressive degradation of the biofilms and prevention of their regeneration. Bacteriophages can also express or induce the expression from within host genome of depolymerizing enzymes that degrade EPS. Nevertheless, they can also infect persister cells, which are dormant variants of regular bacterial cells that are highly resistant to antibiotics. In this case, the lysis process is triggered once persister bacteria are reactivated [96].
\nScientists [97] reported the ability of a bred phage to reduce L-form biofilms formed by
Currently, controlling biofilm formation by nanotechnology-based antimicrobials is of industrial interest, nanoemulsions and nanoparticles (NPs) with antibiofilm activity being an alternative to conventional methods.
\nRecently, some studies made on model system (polystyrene well plates) and real systems (fresh pineapple, tofu, and lettuce) indicated that nanoemulsions of EOs have significantly higher antibiofilm activity compared to pure EOs (Table 2). Antimicrobial efficacy of nanoemulsions is dependent on the droplet size and electrical properties of nanoemulsions [102, 103], nature of bacteria [75, 104], and food matrix [105–107].
\nNanoemulsion | \nParticle size, nm | \nBiofilm-forming bacteria | \nMode of action | \nRef. | \n
---|---|---|---|---|
EO of | \n73 | \n\n | \nInhibit the ability of bacteria to attach to surfaces | \n[185] | \n
EO of | \n78.46 ± 0.51 | \n\n | \nReduce the adhesion of pathogenic bacteria to surfaces | \n[186] | \n
Trans-CA | \n>100 <100 | \n\n | \nMembrane disruption by destabilization of lipids | \n[187] | \n
Linalool | \n10.9 ± 0.1 | \n\n | \nCell membrane integrity disruption | \n[107] | \n
Antibiofilm activity of essential oil (EO) nanoemulsions.
Nanoparticles (NPs) can be used for both inhibition of biofilm formation and eradication of already formed ones [108].
\nIn the last period, NPs with natural compounds gained increased interest because it was demonstrated that the inorganic capsules can protect the natural products with antimicrobial activity [109]. In this respect, cinnamaldehyde-encapsulated chitosan nanoparticles, garlic-silver NPs, and “tree of tee” oil NPs were used to combat biofilm formation by
Metal-based NPs (silver, gold, and metal oxides) with antimicrobial activity can be used to create different nanocomposite materials able to prevent bacterial adhesiveness to food-contact surfaces and equipment. Wu and coworkers [114] showed that cysteine dithiothreitol and beta-mercaptoethanol were able to reduce
The ability of two types of superparamagnetic iron oxide (IONs and IONs coated with 3-aminopropyltriethoxysilane) to inhibit biofilm formation by
Some food technologies belonging to alternative technologies seem to be successful for preventing the biofilm formation and/or for targeting resistant microorganisms and making them more susceptible to molecular interventions in order to hinder their biofilm formation ability. Among these technologies are included plasma treatments, ultrasound treatments, light-based technologies, pulsed electric fields (PEF), and high hydrostatic pressures. With the exception of ultrasound treatments that can be used to fight against biofilms formed on mechanical parts or pipes, the others are mostly applied for food matrix decontamination.
\nPlasma is generated when the added energy ionizes a gas, which is composed of ions, neutrals, and electrons. Plasma treatment is a surface treatment that has a low penetration depth and was reported to be effective against biofilms, depending on the type of surface biofilms are formed on, the distance between plasma and surface, and the thickness or the microbial load.
\nPlasma sources for producing nonthermal plasma at atmospheric pressure are plasma jets, dielectric barrier discharges (DBD), corona discharges, and microwave discharges. Different other characteristics of the plasma have been reported to influence the biofilms’ inactivation such as the setup (electrode configuration), the exposure mode, the operating gas, the frequency, the plasma intensity (voltage), and the time of exposure [122].
\nResearches [123] showed that the efficacy of DBD in-package atmospheric cold plasma (ACP) against
Govaert et al. [122] studied the influence of different plasma characteristics on the inactivation of
It was shown that ACP is a promising technique but alone cannot achieve complete biofilm inactivation and thus it should be complemented by other surface treatments. Possibility to combine ACP with different biocides such as hydrogen peroxide, sodium hypochlorite, ethylenediaminetetraacetic acid, chlorhexidine, octenidine, and polyhexanide applied before or after the plasma treatment was tested by [126] to reduce biofilms cultivated on titanium discs. Also, Gupta et al. [127] studied the antimicrobial effect of an ACP, plasma jet combined with chlorhexidine, for the sterilization of the biofilms formed by
Ultrasound (US) is a form of energy generated by sound waves at frequencies that are too high to be detected by the human hearing (>16 kHz). The US band is also divided into low frequency (16 kHz−1 MHz) and high frequency (>1 MHz) bands.
\nUS was used as biofilm removal method; however, many studies demonstrated that it should be complemented by other inactivation methods [129, 130]. For example, [130] demonstrated that US removed a significant amount of
Combination of US with mild heat and slightly acidic electrolyzed water was used to test the inactivation of
Synergistic effects were registered also for ultrasound (US; 37 kHz, 380 W for 10–60 min) assisted by peroxyacetic acid (PAA; 50–200 ppm) on reducing
The efficacy of US (37 kHz, 200 W, for 30 min)-assisted chemical cleaning methods (10% alcohols, 2.5% benzalkonium chloride, and 2.5% didecyl dimethyl ammonium chloride) for the removal of
Ultraviolet (UV) light technology is based on the emission of radiation within the ultraviolet region (100–400 nm). The antimicrobial behavior of UV light is based on the formation of DNA photoproducts that inhibit transcription and replication and can lead to cell death [135]. Since the absorption of the DNA is in the 200–280 nm range with the maximum at 254 nm, this wavelength of the UV-C range is called germicidal UV light [136].
\nPulsed light (PL) is the next-generation approach to UV delivery. PL is a technology that can be used to decontaminate surfaces by generating short-time high-energy light pulses (millions or thousands of a second) of an intense broad spectrum (200–1100 nm). PL can be used to decontaminate a great variety of foods as well as to decontaminate contact surfaces, thus improving safety in foods and extending their shelf life [137]. The antimicrobial effect is based on strand breaks that lead to the destruction/chemical modification of the DNA and thus prevent the replication of the bacterial cell [138].
\nRecently, Rajkovic and coworkers [139] evaluated the efficacy of pulsed UV light treatments to reduce
A comprehensive review in the literature underlined the various researches directed mainly at inactivation of pathogens in food or on surfaces and for preventing biofilm formation [137]. While there are often considerable differences in the rate of microbial inactivation by PL, a maximum reduction of 3-log was typically achieved, which is below the reduction performance standard of 5-log required by HACCP regulation [138].
\nRegarding the combined methods, synergistic interaction between gallic acid and UV-A light was able to inactivate
However, PL technology limitation related to the inability to effectively treat uneven food surfaces with crevices, the presence of organic material, and large microbial populations generating shading effects should also be taken into account. Future innovation in PL technology will seek to improve fluence efficiency, for example by considering alternative light sources such as LEDs [141], reflective surfaces included in the treatment chamber, using materials such as titanium dioxide to augment irradiation efficacy [138], and other combination of treatments assisted by PL, based on hurdle approach.
\nPulsed electric field (PEF) is a food processing technology that applies short, high-voltage pulses, across a food material placed between two or more electrodes. The pulses enhance cell permeability by damaging the cell membrane, and if the transmembrane potential is sufficiently high, it produces electroporation. Further, if pores are not resealed, it results in cell death. Most of the food applications are designed for liquid flow through pipes where in a certain region the liquid passes in-between the electrodes area that applies the PEFs [142].
\nThermosonication (TS) was investigated in combination with PEF to determine its effects on inactivation and sublethal injury of
PEF demonstrated synergistic potential in combination with additives (EDTA or triethyl citrate) to inactivate
There is a lot of potential demonstrated by PEF and the combination with different other hurdles could contribute to the elimination of persistent clones able to form biofilms.
\nHigh pressure processing (HPP) is a cutting-edge technology that represents an alternative to conventional processing. HPP has the ability to inactivate microorganisms and enzymes and has a minimal impact on sensorial and nutritional properties of food [145, 146].
\nCombined with other different hurdles, the pressure-assisted processing could be oriented toward a more targeted inactivation of pathogens and prevention of biofilm formation.
\nRecent studies were focused on
However,
Understanding better the molecular mechanisms of stress-related genes will allow to better target pathogen inactivation and to select the right hurdle combination and parameters of unconventional technologies to able to reduce the susceptibility of certain pathogens to form biofilms. These types of studies are just at the beginning and many more researches are expected to focus on these topics in the near future.
\nPathogenic and toxigenic bacteria are able to form biofilms, structures that protect the cells and allow them to remain postsanitation in the food processing environment.
\nSpecific genes are expressed in all the steps of biofilm formation or are upregulated under influence of different biotic or abiotic factors. Genes codify for cell surface structures and appendages (flagella, curli, fimbriae, and pili) that are facilitating biofilm formation by helping bacteria to move toward surfaces and to adhere to them, for extracellular polymeric substances that stabilize the biofilms and protect the cells and for quorum sensing communication.
\nScientists developed novel agents and strategies to control biofilm formation or removal. Their application to the food industry would contribute to eradication of undesirable bacteria from food-processing environments and, subsequently, from food products.
\nThis study was supported by a grant of the Executive Agency for Higher Education, Research, Development and Innovation Funding in Romania awarded to the Dunarea de Jos University of Galati (International and European Cooperation—Subprogramme 3.2—Horizon 2020—Contract no: 15/2017), an institution that is a member of the ERA-IB2 consortium “SafeFood” (ID: ERA-IB-16-014).
\nFlorentina Ionela Bucur’s work has been funded by the European Social Fund through the Sectoral Operational Programme Human Capital 2014–2020, through the Financial Agreement with the title “Scholarships for entrepreneurial education among doctoral students and postdoctoral researchers (Be Entrepreneur!),” Contract no. 51680/09.07.2019—SMIS code: 124539.
\nThese Terms and Conditions outline the rules and regulations pertaining to the use of IntechOpen’s website www.intechopen.com and all the subdomains owned by IntechOpen located at 5 Princes Gate Court, London, SW7 2QJ, United Kingdom.
',metaTitle:"Terms and Conditions",metaDescription:"These terms and conditions outline the rules and regulations for the use of IntechOpen Website at https://intechopen.com and all its subdomains owned by Intech Limited located at 7th floor, 10 Lower Thames Street, London, EC3R 6AF, UK.",metaKeywords:null,canonicalURL:"/page/terms-and-conditions",contentRaw:'[{"type":"htmlEditorComponent","content":"By accessing the website at www.intechopen.com you are agreeing to be bound by these Terms of Service, all applicable laws and regulations, and agree that you are responsible for compliance with any applicable local laws. Use and/or access to this site is based on full agreement and compliance of these Terms. All materials contained on this website are protected by applicable copyright and trademark laws.
\\n\\nThe following terminology applies to these Terms and Conditions, Privacy Statement, Disclaimer Notice, and any or all Agreements:
\\n\\n“Client”, “Customer”, “You” and “Your” refers to you, the person accessing this website and accepting the Company’s Terms and Conditions;
\\n\\n“The Company”, “Ourselves”, “We”, “Our” and “Us”, refers to our Company, IntechOpen;
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\\n\\nAny use of the above terminology, or other words in the singular, plural, capitalization and/or he/she or they, are taken as interchangeable.
\\n\\nUnless otherwise stated, IntechOpen and/or its licensors own the intellectual property rights for all materials on www.intechopen.com. All intellectual property rights are reserved. You may view, download, share, link and print pages from www.intechopen.com for your own personal use, subject to the restrictions set out in these Terms and Conditions.
\\n\\nWe employ the use of cookies. By using the IntechOpen website you consent to the use of cookies in accordance with IntechOpen’s Privacy Policy. Most modern day interactive websites use cookies to enable the retrieval of user details for each visit. On our site, cookies are predominantly used to enable functionality and ease of use for those visiting the site.
\\n\\nIn no circumstances shall IntechOpen or its suppliers be liable for any damages (including, without limitation, damages for loss of data or profit, or due to business interruption) arising out of the use, or inability to use, the materials on IntechOpen's websites, even if IntechOpen or an IntechOpen authorized representative has been notified orally or in writing of the possibility of such damage. Some jurisdictions do not allow limitations on implied warranties, or limitations of liability for consequential or incidental damages; consequently, these limitations may not apply to you.
\\n\\nIntechopen.com website content and services are provided on an "AS IS" and an "AS AVAILABLE" basis. Material appearing on www.intechopen.com could include minor technical, typographical, or photographic errors. IntechOpen may make changes to any material contained on its website at any time without notice.
\\n\\nIntechOpen has no formal affiliation to any external sites that link to www.intechopen.com, unless otherwise specifically stated. As such, it is not responsible for content that appears on any such sites. The inclusion of any link to IntechOpen does not imply endorsement by IntechOpen. Use of any such linked website is done solely at the user's own discretion.
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\\n\\nIf you find any link on our website, or any linked website, objectionable for any reason, please Contact Us. We will consider all requests to remove links but will have no obligation to do so.
\\n\\nWithout prior approval and express written permission, you may not create frames around our web pages or use other techniques that alter in any way the visual presentation or appearance of our website.
\\n\\nIntechOpen may revise its Terms of Service for its website at any time without notice. By using this website, you are agreeing to be bound by the current version of all Terms at the time of use.
\\n\\nThese Terms and Conditions are governed by and construed in accordance with the laws of the United Kingdom and you irrevocably submit to the exclusive jurisdiction of the courts in London, United Kingdom.
\\n\\nCroatian version of Terms and Conditions available here
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\n\nThe following terminology applies to these Terms and Conditions, Privacy Statement, Disclaimer Notice, and any or all Agreements:
\n\n“Client”, “Customer”, “You” and “Your” refers to you, the person accessing this website and accepting the Company’s Terms and Conditions;
\n\n“The Company”, “Ourselves”, “We”, “Our” and “Us”, refers to our Company, IntechOpen;
\n\n“Party”, “Parties”, or “Us”, refers to both the Client and ourselves, or either the Client or ourselves.
\n\nAll Terms refer to the offer, acceptance, and consideration of payment necessary to provide assistance to the Client in the most appropriate manner, whether by formal meetings of a fixed duration, or by any other agreed means, for the express purpose of meeting the Client’s needs in respect of provision of the Company’s stated services/products, and in accordance with, and subject to, the prevailing laws of the United Kingdom.
\n\nAny use of the above terminology, or other words in the singular, plural, capitalization and/or he/she or they, are taken as interchangeable.
\n\nUnless otherwise stated, IntechOpen and/or its licensors own the intellectual property rights for all materials on www.intechopen.com. All intellectual property rights are reserved. You may view, download, share, link and print pages from www.intechopen.com for your own personal use, subject to the restrictions set out in these Terms and Conditions.
\n\nWe employ the use of cookies. By using the IntechOpen website you consent to the use of cookies in accordance with IntechOpen’s Privacy Policy. Most modern day interactive websites use cookies to enable the retrieval of user details for each visit. On our site, cookies are predominantly used to enable functionality and ease of use for those visiting the site.
\n\nIn no circumstances shall IntechOpen or its suppliers be liable for any damages (including, without limitation, damages for loss of data or profit, or due to business interruption) arising out of the use, or inability to use, the materials on IntechOpen's websites, even if IntechOpen or an IntechOpen authorized representative has been notified orally or in writing of the possibility of such damage. Some jurisdictions do not allow limitations on implied warranties, or limitations of liability for consequential or incidental damages; consequently, these limitations may not apply to you.
\n\nIntechopen.com website content and services are provided on an "AS IS" and an "AS AVAILABLE" basis. Material appearing on www.intechopen.com could include minor technical, typographical, or photographic errors. IntechOpen may make changes to any material contained on its website at any time without notice.
\n\nIntechOpen has no formal affiliation to any external sites that link to www.intechopen.com, unless otherwise specifically stated. As such, it is not responsible for content that appears on any such sites. The inclusion of any link to IntechOpen does not imply endorsement by IntechOpen. Use of any such linked website is done solely at the user's own discretion.
\n\nWe reserve the right of ownership over our entire website www.intechopen.com, and all contents. By using our services, you agree to remove all links to our website immediately upon request. We also reserve the right to amend these Terms and Conditions and our linking policy at any time. By continuing to link to our website, you agree to be bound to, and abide by, these linking Terms and Conditions.
\n\nIf you find any link on our website, or any linked website, objectionable for any reason, please Contact Us. We will consider all requests to remove links but will have no obligation to do so.
\n\nWithout prior approval and express written permission, you may not create frames around our web pages or use other techniques that alter in any way the visual presentation or appearance of our website.
\n\nIntechOpen may revise its Terms of Service for its website at any time without notice. By using this website, you are agreeing to be bound by the current version of all Terms at the time of use.
\n\nThese Terms and Conditions are governed by and construed in accordance with the laws of the United Kingdom and you irrevocably submit to the exclusive jurisdiction of the courts in London, United Kingdom.
\n\nCroatian version of Terms and Conditions available here
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He is a full professor of signal processing and pattern recognition and is head of the Signals and Communications Department at ULPGC, teaching from 2001 on subjects on signal processing and learning theory. His research lines are biometrics, biomedical signals and images, data mining, classification system, signal and image processing, machine learning, and environmental intelligence. He has researched in 52 international and Spanish research projects, some of them as head researcher. He is co-author of 4 books, co-editor of 27 proceedings books, guest editor for 8 JCR-ISI international journals, and up to 24 book chapters. He has over 450 papers published in international journals and conferences (81 of them indexed on JCR – ISI - Web of Science). He has published seven patents in the Spanish Patent and Trademark Office. He has been a supervisor on 8 Ph.D. theses (11 more are under supervision), and 130 master theses. 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Dr. Mohammadi received his BSc. and MSc. degrees in Mechanical Engineering from Sharif University of Technology, Tehran, Iran, and his PhD. degree in Biomedical Engineering (biomaterials) from the University of Western Ontario. He was a postdoctoral trainee for almost four years at University of Calgary and Harvard Medical School. He is an industry innovator having created the technology to produce lifelike synthetic platforms that can be used for the simulation of almost all cardiovascular reconstructive surgeries. He’s been heavily involved in the design and development of cardiovascular devices and technology for the past 10 years. He is currently an Assistant Professor with the University of British Colombia, Canada.",institutionString:"University of British Columbia",institution:{name:"University of British Columbia",institutionURL:null,country:{name:"Canada"}}}]}]},openForSubmissionBooks:{paginationCount:3,paginationItems:[{id:"11570",title:"Influenza - New Approaches",coverURL:"https://cdn.intechopen.com/books/images_new/11570.jpg",hash:"157b379b9d7a4bf5e2cc7a742f155a44",secondStepPassed:!0,currentStepOfPublishingProcess:3,submissionDeadline:"May 10th 2022",isOpenForSubmission:!0,editors:[{id:"139889",title:"Dr.",name:"Seyyed Shamsadin",surname:"Athari",slug:"seyyed-shamsadin-athari",fullName:"Seyyed Shamsadin Athari"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null},{id:"11569",title:"Bacterial Sexually Transmitted Infections - New Findings, Diagnosis, Treatment, and Prevention",coverURL:"https://cdn.intechopen.com/books/images_new/11569.jpg",hash:"069d6142ecb0d46d14920102d48c0e9d",secondStepPassed:!0,currentStepOfPublishingProcess:3,submissionDeadline:"May 31st 2022",isOpenForSubmission:!0,editors:[{id:"189561",title:"Dr.",name:"Mihaela Laura",surname:"Vica",slug:"mihaela-laura-vica",fullName:"Mihaela Laura Vica"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null},{id:"11568",title:"Staphylococcal Infections - Recent Advances and Perspectives",coverURL:"https://cdn.intechopen.com/books/images_new/11568.jpg",hash:"92c881664d1921c7f2d0fee34b78cd08",secondStepPassed:!1,currentStepOfPublishingProcess:2,submissionDeadline:"July 8th 2022",isOpenForSubmission:!0,editors:[{id:"59719",title:"Dr.",name:"Jaime",surname:"Bustos-Martínez",slug:"jaime-bustos-martinez",fullName:"Jaime Bustos-Martínez"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null}]},onlineFirstChapters:{},subseriesFiltersForOFChapters:[],publishedBooks:{},subseriesFiltersForPublishedBooks:[],publicationYearFilters:[],authors:{paginationCount:301,paginationItems:[{id:"116250",title:"Dr.",name:"Nima",middleName:null,surname:"Rezaei",slug:"nima-rezaei",fullName:"Nima Rezaei",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/116250/images/system/116250.jpg",biography:"Professor Nima Rezaei obtained an MD from Tehran University of Medical Sciences, Iran. He also obtained an MSc in Molecular and Genetic Medicine, and a Ph.D. in Clinical Immunology and Human Genetics from the University of Sheffield, UK. He also completed a short-term fellowship in Pediatric Clinical Immunology and Bone Marrow Transplantation at Newcastle General Hospital, England. Dr. Rezaei is a Full Professor of Immunology and Vice Dean of International Affairs and Research, at the School of Medicine, Tehran University of Medical Sciences, and the co-founder and head of the Research Center for Immunodeficiencies. He is also the founding president of the Universal Scientific Education and Research Network (USERN). Dr. Rezaei has directed more than 100 research projects and has designed and participated in several international collaborative projects. He is an editor, editorial assistant, or editorial board member of more than forty international journals. He has edited more than 50 international books, presented more than 500 lectures/posters in congresses/meetings, and published more than 1,100 scientific papers in international journals.",institutionString:"Tehran University of Medical Sciences",institution:{name:"Tehran University of Medical Sciences",country:{name:"Iran"}}},{id:"180733",title:"Dr.",name:"Jean",middleName:null,surname:"Engohang-Ndong",slug:"jean-engohang-ndong",fullName:"Jean Engohang-Ndong",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/180733/images/system/180733.png",biography:"Dr. Jean Engohang-Ndong was born and raised in Gabon. After obtaining his Associate Degree of Science at the University of Science and Technology of Masuku, Gabon, he continued his education in France where he obtained his BS, MS, and Ph.D. in Medical Microbiology. He worked as a post-doctoral fellow at the Public Health Research Institute (PHRI), Newark, NJ for four years before accepting a three-year faculty position at Brigham Young University-Hawaii. Dr. Engohang-Ndong is a tenured faculty member with the academic rank of Full Professor at Kent State University, Ohio, where he teaches a wide range of biological science courses and pursues his research in medical and environmental microbiology. Recently, he expanded his research interest to epidemiology and biostatistics of chronic diseases in Gabon.",institutionString:"Kent State University",institution:{name:"Kent State University",country:{name:"United States of America"}}},{id:"188773",title:"Prof.",name:"Emmanuel",middleName:null,surname:"Drouet",slug:"emmanuel-drouet",fullName:"Emmanuel Drouet",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/188773/images/system/188773.png",biography:"Emmanuel Drouet, PharmD, is a Professor of Virology at the Faculty of Pharmacy, the University Grenoble-Alpes, France. As a head scientist at the Institute of Structural Biology in Grenoble, Dr. Drouet’s research investigates persisting viruses in humans (RNA and DNA viruses) and the balance with our host immune system. He focuses on these viruses’ effects on humans (both their impact on pathology and their symbiotic relationships in humans). He has an excellent track record in the herpesvirus field, and his group is engaged in clinical research in the field of Epstein-Barr virus diseases. He is the editor of the online Encyclopedia of Environment and he coordinates the Universal Health Coverage education program for the BioHealth Computing Schools of the European Institute of Science.",institutionString:null,institution:{name:"Grenoble Alpes University",country:{name:"France"}}},{id:"131400",title:"Prof.",name:"Alfonso J.",middleName:null,surname:"Rodriguez-Morales",slug:"alfonso-j.-rodriguez-morales",fullName:"Alfonso J. Rodriguez-Morales",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/131400/images/system/131400.png",biography:"Dr. Rodriguez-Morales is an expert in tropical and emerging diseases, particularly zoonotic and vector-borne diseases (especially arboviral diseases). He is the president of the Travel Medicine Committee of the Pan-American Infectious Diseases Association (API), as well as the president of the Colombian Association of Infectious Diseases (ACIN). He is a member of the Committee on Tropical Medicine, Zoonoses, and Travel Medicine of ACIN. He is a vice-president of the Latin American Society for Travel Medicine (SLAMVI) and a Member of the Council of the International Society for Infectious Diseases (ISID). Since 2014, he has been recognized as a Senior Researcher, at the Ministry of Science of Colombia. He is a professor at the Faculty of Medicine of the Fundacion Universitaria Autonoma de las Americas, in Pereira, Risaralda, Colombia. He is an External Professor, Master in Research on Tropical Medicine and International Health, Universitat de Barcelona, Spain. He is also a professor at the Master in Clinical Epidemiology and Biostatistics, Universidad Científica del Sur, Lima, Peru. In 2021 he has been awarded the “Raul Isturiz Award” Medal of the API. Also, in 2021, he was awarded with the “Jose Felix Patiño” Asclepius Staff Medal of the Colombian Medical College, due to his scientific contributions to COVID-19 during the pandemic. He is currently the Editor in Chief of the journal Travel Medicine and Infectious Diseases. His Scopus H index is 47 (Google Scholar H index, 68).",institutionString:"Institución Universitaria Visión de las Américas, Colombia",institution:null},{id:"332819",title:"Dr.",name:"Chukwudi Michael",middleName:"Michael",surname:"Egbuche",slug:"chukwudi-michael-egbuche",fullName:"Chukwudi Michael Egbuche",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/332819/images/14624_n.jpg",biography:"I an Dr. Chukwudi Michael Egbuche. I am a Senior Lecturer in the Department of Parasitology and Entomology, Nnamdi Azikiwe University, Awka.",institutionString:null,institution:{name:"Nnamdi Azikiwe University",country:{name:"Nigeria"}}},{id:"284232",title:"Mr.",name:"Nikunj",middleName:"U",surname:"Tandel",slug:"nikunj-tandel",fullName:"Nikunj Tandel",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/284232/images/8275_n.jpg",biography:'Mr. Nikunj Tandel has completed his Master\'s degree in Biotechnology from VIT University, India in the year of 2012. He is having 8 years of research experience especially in the field of malaria epidemiology, immunology, and nanoparticle-based drug delivery system against the infectious diseases, autoimmune disorders and cancer. He has worked for the NIH funded-International Center of Excellence in Malaria Research project "Center for the study of complex malaria in India (CSCMi)" in collaboration with New York University. The preliminary objectives of the study are to understand and develop the evidence-based tools and interventions for the control and prevention of malaria in different sites of the INDIA. Alongside, with the help of next-generation genomics study, the team has studied the antimalarial drug resistance in India. Further, he has extended his research in the development of Humanized mice for the study of liver-stage malaria and identification of molecular marker(s) for the Artemisinin resistance. At present, his research focuses on understanding the role of B cells in the activation of CD8+ T cells in malaria. Received the CSIR-SRF (Senior Research Fellow) award-2018, FIMSA (Federation of Immunological Societies of Asia-Oceania) Travel Bursary award to attend the IUIS-IIS-FIMSA Immunology course-2019',institutionString:"Nirma University",institution:{name:"Nirma University",country:{name:"India"}}},{id:"334383",title:"Ph.D.",name:"Simone",middleName:"Ulrich",surname:"Ulrich Picoli",slug:"simone-ulrich-picoli",fullName:"Simone Ulrich Picoli",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/334383/images/15919_n.jpg",biography:"Graduated in Pharmacy from Universidade Luterana do Brasil (1999), Master in Agricultural and Environmental Microbiology from Federal University of Rio Grande do Sul (2002), Specialization in Clinical Microbiology from Universidade de São Paulo, USP (2007) and PhD in Sciences in Gastroenterology and Hepatology (2012). She is currently an Adjunct Professor at Feevale University in Medicine and Biomedicine courses and a permanent professor of the Academic Master\\'s Degree in Virology. She has experience in the field of Microbiology, with an emphasis on Bacteriology, working mainly on the following topics: bacteriophages, bacterial resistance, clinical microbiology and food microbiology.",institutionString:null,institution:{name:"Universidade Feevale",country:{name:"Brazil"}}},{id:"229220",title:"Dr.",name:"Amjad",middleName:"Islam",surname:"Aqib",slug:"amjad-aqib",fullName:"Amjad Aqib",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/229220/images/system/229220.png",biography:"Dr. Amjad Islam Aqib obtained a DVM and MSc (Hons) from University of Agriculture Faisalabad (UAF), Pakistan, and a PhD from the University of Veterinary and Animal Sciences Lahore, Pakistan. Dr. Aqib joined the Department of Clinical Medicine and Surgery at UAF for one year as an assistant professor where he developed a research laboratory designated for pathogenic bacteria. Since 2018, he has been Assistant Professor/Officer in-charge, Department of Medicine, Manager Research Operations and Development-ORIC, and President One Health Club at Cholistan University of Veterinary and Animal Sciences, Bahawalpur, Pakistan. He has nearly 100 publications to his credit. His research interests include epidemiological patterns and molecular analysis of antimicrobial resistance and modulation and vaccine development against animal pathogens of public health concern.",institutionString:"Cholistan University of Veterinary and Animal Sciences",institution:null},{id:"333753",title:"Dr.",name:"Rais",middleName:null,surname:"Ahmed",slug:"rais-ahmed",fullName:"Rais Ahmed",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/333753/images/20168_n.jpg",biography:null,institutionString:null,institution:{name:"University of Agriculture Faisalabad",country:{name:"Pakistan"}}},{id:"62900",title:"Prof.",name:"Fethi",middleName:null,surname:"Derbel",slug:"fethi-derbel",fullName:"Fethi Derbel",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/62900/images/system/62900.jpeg",biography:"Professor Fethi Derbel was born in 1960 in Tunisia. He received his medical degree from the Sousse Faculty of Medicine at Sousse, University of Sousse, Tunisia. He completed his surgical residency in General Surgery at the University Hospital Farhat Hached of Sousse and was a member of the Unit of Liver Transplantation in the University of Rennes, France. He then worked in the Department of Surgery at the Sahloul University Hospital in Sousse. Professor Derbel is presently working at the Clinique les Oliviers, Sousse, Tunisia. His hospital activities are mostly concerned with laparoscopic, colorectal, pancreatic, hepatobiliary, and gastric surgery. He is also very interested in hernia surgery and performs ventral hernia repairs and inguinal hernia repairs. He has been a member of the GREPA and Tunisian Hernia Society (THS). During his residency, he managed patients suffering from diabetic foot, and he was very interested in this pathology. For this reason, he decided to coordinate a book project dealing with the diabetic foot. Professor Derbel has published many articles in journals and collaborates intensively with IntechOpen Access Publisher as an editor.",institutionString:"Clinique les Oliviers",institution:null},{id:"300144",title:"Dr.",name:"Meriem",middleName:null,surname:"Braiki",slug:"meriem-braiki",fullName:"Meriem Braiki",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/300144/images/system/300144.jpg",biography:"Dr. Meriem Braiki is a specialist in pediatric surgeon from Tunisia. She was born in 1985. She received her medical degree from the University of Medicine at Sousse, Tunisia. She achieved her surgical residency training periods in Pediatric Surgery departments at University Hospitals in Monastir, Tunis and France.\r\nShe is currently working at the Pediatric surgery department, Sidi Bouzid Hospital, Tunisia. Her hospital activities are mostly concerned with laparoscopic, parietal, urological and digestive surgery. She has published several articles in diffrent journals.",institutionString:"Sidi Bouzid Regional Hospital",institution:null},{id:"229481",title:"Dr.",name:"Erika M.",middleName:"Martins",surname:"de Carvalho",slug:"erika-m.-de-carvalho",fullName:"Erika M. de Carvalho",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/229481/images/6397_n.jpg",biography:null,institutionString:null,institution:{name:"Oswaldo Cruz Foundation",country:{name:"Brazil"}}},{id:"186537",title:"Prof.",name:"Tonay",middleName:null,surname:"Inceboz",slug:"tonay-inceboz",fullName:"Tonay Inceboz",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/186537/images/system/186537.jfif",biography:"I was graduated from Ege University of Medical Faculty (Turkey) in 1988 and completed his Med. PhD degree in Medical Parasitology at the same university. I became an Associate Professor in 2008 and Professor in 2014. I am currently working as a Professor at the Department of Medical Parasitology at Dokuz Eylul University, Izmir, Turkey.\n\nI have given many lectures, presentations in different academic meetings. I have more than 60 articles in peer-reviewed journals, 18 book chapters, 1 book editorship.\n\nMy research interests are Echinococcus granulosus, Echinococcus multilocularis (diagnosis, life cycle, in vitro and in vivo cultivation), and Trichomonas vaginalis (diagnosis, PCR, and in vitro cultivation).",institutionString:"Dokuz Eylül University",institution:{name:"Dokuz Eylül University",country:{name:"Turkey"}}},{id:"71812",title:"Prof.",name:"Hanem Fathy",middleName:"Fathy",surname:"Khater",slug:"hanem-fathy-khater",fullName:"Hanem Fathy Khater",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/71812/images/1167_n.jpg",biography:"Prof. Khater is a Professor of Parasitology at Benha University, Egypt. She studied for her doctoral degree, at the Department of Entomology, College of Agriculture, Food and Natural Resources, University of Missouri, Columbia, USA. She has completed her Ph.D. degrees in Parasitology in Egypt, from where she got the award for “the best scientific Ph.D. dissertation”. She worked at the School of Biological Sciences, Bristol, England, the UK in controlling insects of medical and veterinary importance as a grant from Newton Mosharafa, the British Council. Her research is focused on searching of pesticides against mosquitoes, house flies, lice, green bottle fly, camel nasal botfly, soft and hard ticks, mites, and the diamondback moth as well as control of several parasites using safe and natural materials to avoid drug resistances and environmental contamination.",institutionString:null,institution:{name:"Banha University",country:{name:"Egypt"}}},{id:"99780",title:"Prof.",name:"Omolade",middleName:"Olayinka",surname:"Okwa",slug:"omolade-okwa",fullName:"Omolade Okwa",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/99780/images/system/99780.jpg",biography:"Omolade Olayinka Okwa is presently a Professor of Parasitology at Lagos State University, Nigeria. She has a PhD in Parasitology (1997), an MSc in Cellular Parasitology (1992), and a BSc (Hons) Zoology (1990) all from the University of Ibadan, Nigeria. She teaches parasitology at the undergraduate and postgraduate levels. She was a recipient of a Commonwealth fellowship supported by British Council tenable at the Centre for Entomology and Parasitology (CAEP), Keele University, United Kingdom between 2004 and 2005. She was awarded an Honorary Visiting Research Fellow at the same university from 2005 to 2007. \nShe has been an external examiner to the Department of Veterinary Microbiology and Parasitology, University of Ibadan, MSc programme between 2010 and 2012. She is a member of the Nigerian Society of Experimental Biology (NISEB), Parasitology and Public Health Society of Nigeria (PPSN), Science Association of Nigeria (SAN), Zoological Society of Nigeria (ZSN), and is Vice Chairperson of the Organisation of Women in Science (OWSG), LASU chapter. She served as Head of Department of Zoology and Environmental Biology, Lagos State University from 2007 to 2010 and 2014 to 2016. She is a reviewer for several local and international journals such as Unilag Journal of Science, Libyan Journal of Medicine, Journal of Medicine and Medical Sciences, and Annual Research and Review in Science. \nShe has authored 45 scientific research publications in local and international journals, 8 scientific reviews, 4 books, and 3 book chapters, which includes the books “Malaria Parasites” and “Malaria” which are IntechOpen access publications.",institutionString:"Lagos State University",institution:{name:"Lagos State University",country:{name:"Nigeria"}}},{id:"273100",title:"Dr.",name:"Vijay",middleName:null,surname:"Gayam",slug:"vijay-gayam",fullName:"Vijay Gayam",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/273100/images/system/273100.jpeg",biography:"Dr. Vijay Bhaskar Reddy Gayam is currently practicing as an internist at Interfaith Medical Center in Brooklyn, New York, USA. He is also a Clinical Assistant Professor at the SUNY Downstate University Hospital and Adjunct Professor of Medicine at the American University of Antigua. He is a holder of an M.B.B.S. degree bestowed to him by Osmania Medical College and received his M.D. at Interfaith Medical Center. His career goals thus far have heavily focused on direct patient care, medical education, and clinical research. He currently serves in two leadership capacities; Assistant Program Director of Medicine at Interfaith Medical Center and as a Councilor for the American\r\nFederation for Medical Research. As a true academician and researcher, he has more than 50 papers indexed in international peer-reviewed journals. He has also presented numerous papers in multiple national and international scientific conferences. His areas of research interest include general internal medicine, gastroenterology and hepatology. He serves as an editor, editorial board member and reviewer for multiple international journals. His research on Hepatitis C has been very successful and has led to multiple research awards, including the 'Equity in Prevention and Treatment Award” from the New York Department of Health Viral Hepatitis Symposium (2018) and the 'Presidential Poster Award” awarded to him by the American College of Gastroenterology (2018). He was also awarded 'Outstanding Clinician in General Medicine” by Venus International Foundation for his extensive research expertise and services, perform over and above the standard expected in the advancement of healthcare, patient safety and quality of care.",institutionString:"Interfaith Medical Center",institution:{name:"Interfaith Medical Center",country:{name:"United States of America"}}},{id:"93517",title:"Dr.",name:"Clement",middleName:"Adebajo",surname:"Meseko",slug:"clement-meseko",fullName:"Clement Meseko",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/93517/images/system/93517.jpg",biography:"Dr. Clement Meseko obtained DVM and PhD degree in Veterinary Medicine and Virology respectively. He has worked for over 20 years in both private and public sectors including the academia, contributing to knowledge and control of infectious disease. Through the application of epidemiological skill, classical and molecular virological skills, he investigates viruses of economic and public health importance for the mitigation of the negative impact on people, animal and the environment in the context of Onehealth. \r\nDr. Meseko’s field experience on animal and zoonotic diseases and pathogen dynamics at the human-animal interface over the years shaped his carrier in research and scientific inquiries. He has been part of the investigation of Highly Pathogenic Avian Influenza incursions in sub Saharan Africa and monitors swine Influenza (Pandemic influenza Virus) agro-ecology and potential for interspecies transmission. He has authored and reviewed a number of journal articles and book chapters.",institutionString:"National Veterinary Research Institute",institution:{name:"National Veterinary Research Institute",country:{name:"Nigeria"}}},{id:"158026",title:"Prof.",name:"Shailendra K.",middleName:null,surname:"Saxena",slug:"shailendra-k.-saxena",fullName:"Shailendra K. Saxena",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRET3QAO/Profile_Picture_2022-05-10T10:10:26.jpeg",biography:"Professor Dr. Shailendra K. Saxena is a vice dean and professor at King George's Medical University, Lucknow, India. His research interests involve understanding the molecular mechanisms of host defense during human viral infections and developing new predictive, preventive, and therapeutic strategies for them using Japanese encephalitis virus (JEV), HIV, and emerging viruses as a model via stem cell and cell culture technologies. His research work has been published in various high-impact factor journals (Science, PNAS, Nature Medicine) with a high number of citations. He has received many awards and honors in India and abroad including various Young Scientist Awards, BBSRC India Partnering Award, and Dr. JC Bose National Award of Department of Biotechnology, Min. of Science and Technology, Govt. of India. Dr. Saxena is a fellow of various international societies/academies including the Royal College of Pathologists, United Kingdom; Royal Society of Medicine, London; Royal Society of Biology, United Kingdom; Royal Society of Chemistry, London; and Academy of Translational Medicine Professionals, Austria. He was named a Global Leader in Science by The Scientist. He is also an international opinion leader/expert in vaccination for Japanese encephalitis by IPIC (UK).",institutionString:"King George's Medical University",institution:{name:"King George's Medical University",country:{name:"India"}}},{id:"94928",title:"Dr.",name:"Takuo",middleName:null,surname:"Mizukami",slug:"takuo-mizukami",fullName:"Takuo Mizukami",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/94928/images/6402_n.jpg",biography:null,institutionString:null,institution:{name:"National Institute of Infectious Diseases",country:{name:"Japan"}}},{id:"233433",title:"Dr.",name:"Yulia",middleName:null,surname:"Desheva",slug:"yulia-desheva",fullName:"Yulia Desheva",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/233433/images/system/233433.png",biography:"Dr. Yulia Desheva is a leading researcher at the Institute of Experimental Medicine, St. Petersburg, Russia. She is a professor in the Stomatology Faculty, St. Petersburg State University. She has expertise in the development and evaluation of a wide range of live mucosal vaccines against influenza and bacterial complications. Her research interests include immunity against influenza and COVID-19 and the development of immunization schemes for high-risk individuals.",institutionString:'Federal State Budgetary Scientific Institution "Institute of Experimental Medicine"',institution:null},{id:"238958",title:"Mr.",name:"Atamjit",middleName:null,surname:"Singh",slug:"atamjit-singh",fullName:"Atamjit Singh",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/238958/images/6575_n.jpg",biography:null,institutionString:null,institution:null},{id:"252058",title:"M.Sc.",name:"Juan",middleName:null,surname:"Sulca",slug:"juan-sulca",fullName:"Juan Sulca",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/252058/images/12834_n.jpg",biography:null,institutionString:null,institution:null},{id:"191392",title:"Dr.",name:"Marimuthu",middleName:null,surname:"Govindarajan",slug:"marimuthu-govindarajan",fullName:"Marimuthu Govindarajan",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/191392/images/5828_n.jpg",biography:"Dr. M. Govindarajan completed his BSc degree in Zoology at Government Arts College (Autonomous), Kumbakonam, and MSc, MPhil, and PhD degrees at Annamalai University, Annamalai Nagar, Tamil Nadu, India. He is serving as an assistant professor at the Department of Zoology, Annamalai University. His research interests include isolation, identification, and characterization of biologically active molecules from plants and microbes. He has identified more than 20 pure compounds with high mosquitocidal activity and also conducted high-quality research on photochemistry and nanosynthesis. He has published more than 150 studies in journals with impact factor and 2 books in Lambert Academic Publishing, Germany. He serves as an editorial board member in various national and international scientific journals.",institutionString:null,institution:null},{id:"274660",title:"Dr.",name:"Damodar",middleName:null,surname:"Paudel",slug:"damodar-paudel",fullName:"Damodar Paudel",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/274660/images/8176_n.jpg",biography:"I am DrDamodar Paudel,currently working as consultant Physician in Nepal police Hospital.",institutionString:null,institution:null},{id:"241562",title:"Dr.",name:"Melvin",middleName:null,surname:"Sanicas",slug:"melvin-sanicas",fullName:"Melvin Sanicas",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/241562/images/6699_n.jpg",biography:null,institutionString:null,institution:null},{id:"337446",title:"Dr.",name:"Maria",middleName:null,surname:"Zavala-Colon",slug:"maria-zavala-colon",fullName:"Maria Zavala-Colon",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"University of Puerto Rico, Medical Sciences Campus",country:{name:"United States of America"}}},{id:"338856",title:"Mrs.",name:"Nur Alvira",middleName:null,surname:"Pascawati",slug:"nur-alvira-pascawati",fullName:"Nur Alvira Pascawati",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Universitas Respati Yogyakarta",country:{name:"Indonesia"}}},{id:"441116",title:"Dr.",name:"Jovanka M.",middleName:null,surname:"Voyich",slug:"jovanka-m.-voyich",fullName:"Jovanka M. 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Dr Ventura also holds the positions of Affiliated Professor at Virginia Commonwealth University (Richmond, USA) and Distinguished Adjunct Professor at King Abdulaziz University (Jeddah, Saudi Arabia). Additionally, he is deputy director of the Andalusian Research Institute in Data Science and Computational Intelligence (DaSCI) and heads the Knowledge Discovery and Intelligent Systems Research Laboratory. He has published more than ten books and over 300 articles in journals and scientific conferences. Currently, his work has received over 18,000 citations according to Google Scholar, including more than 2200 citations in 2020. In the last five years, he has published more than 60 papers in international journals indexed in the JCR (around 70% of them belonging to first quartile journals) and he has edited some Springer books “Supervised Descriptive Pattern Mining” (2018), “Multiple Instance Learning - Foundations and Algorithms” (2016), and “Pattern Mining with Evolutionary Algorithms” (2016). He has also been involved in more than 20 research projects supported by the Spanish and Andalusian governments and the European Union. He currently belongs to the editorial board of PeerJ Computer Science, Information Fusion and Engineering Applications of Artificial Intelligence journals, being also associate editor of Applied Computational Intelligence and Soft Computing and IEEE Transactions on Cybernetics. Finally, he is editor-in-chief of Progress in Artificial Intelligence. 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