Chromatographic and spectrometric methodologies used recently in lipoproteomic analysis.
\\n\\n
Released this past November, the list is based on data collected from the Web of Science and highlights some of the world’s most influential scientific minds by naming the researchers whose publications over the previous decade have included a high number of Highly Cited Papers placing them among the top 1% most-cited.
\\n\\nWe wish to congratulate all of the researchers named and especially our authors on this amazing accomplishment! We are happy and proud to share in their success!
Note: Edited in March 2021
\\n"}]',published:!0,mainMedia:{caption:"Highly Cited",originalUrl:"/media/original/117"}},components:[{type:"htmlEditorComponent",content:'IntechOpen is proud to announce that 191 of our authors have made the Clarivate™ Highly Cited Researchers List for 2020, ranking them among the top 1% most-cited.
\n\nThroughout the years, the list has named a total of 261 IntechOpen authors as Highly Cited. Of those researchers, 69 have been featured on the list multiple times.
\n\n\n\nReleased this past November, the list is based on data collected from the Web of Science and highlights some of the world’s most influential scientific minds by naming the researchers whose publications over the previous decade have included a high number of Highly Cited Papers placing them among the top 1% most-cited.
\n\nWe wish to congratulate all of the researchers named and especially our authors on this amazing accomplishment! We are happy and proud to share in their success!
Note: Edited in March 2021
\n'}],latestNews:[{slug:"intechopen-supports-asapbio-s-new-initiative-publish-your-reviews-20220729",title:"IntechOpen Supports ASAPbio’s New Initiative Publish Your Reviews"},{slug:"webinar-introduction-to-open-science-wednesday-18-may-1-pm-cest-20220518",title:"Webinar: Introduction to Open Science | Wednesday 18 May, 1 PM CEST"},{slug:"step-in-the-right-direction-intechopen-launches-a-portfolio-of-open-science-journals-20220414",title:"Step in the Right Direction: IntechOpen Launches a Portfolio of Open Science Journals"},{slug:"let-s-meet-at-london-book-fair-5-7-april-2022-olympia-london-20220321",title:"Let’s meet at London Book Fair, 5-7 April 2022, Olympia London"},{slug:"50-books-published-as-part-of-intechopen-and-knowledge-unlatched-ku-collaboration-20220316",title:"50 Books published as part of IntechOpen and Knowledge Unlatched (KU) Collaboration"},{slug:"intechopen-joins-the-united-nations-sustainable-development-goals-publishers-compact-20221702",title:"IntechOpen joins the United Nations Sustainable Development Goals Publishers Compact"},{slug:"intechopen-signs-exclusive-representation-agreement-with-lsr-libros-servicios-y-representaciones-s-a-de-c-v-20211123",title:"IntechOpen Signs Exclusive Representation Agreement with LSR Libros Servicios y Representaciones S.A. de C.V"},{slug:"intechopen-expands-partnership-with-research4life-20211110",title:"IntechOpen Expands Partnership with Research4Life"}]},book:{item:{type:"book",id:"5361",leadTitle:null,fullTitle:"Energy Efficient Buildings",title:"Energy Efficient Buildings",subtitle:null,reviewType:"peer-reviewed",abstract:"This book discusses energy efficient buildings and the role they play in our efforts to address climate change, energy consumption and greenhouse gas emissions by considering buildings and the construction sector's unique position along a critical path to decarbonisation from a multi-perspective and holistic viewpoint. Topics covered in the book range from daylighting, building topology comparison, building envelope design, zero energy homes in hot arid regions, life-cycle considerations and energy efficiency analysis to managing energy demand through equipment selection. Each chapter addresses an important aspect of energy efficient building and serves as a vital building block towards constructing a timely and relevant body of knowledge in energy efficient buildings.",isbn:"978-953-51-2876-2",printIsbn:"978-953-51-2875-5",pdfIsbn:"978-953-51-6691-7",doi:"10.5772/62592",price:119,priceEur:129,priceUsd:155,slug:"energy-efficient-buildings",numberOfPages:214,isOpenForSubmission:!1,isInWos:null,isInBkci:!1,hash:"b479633e09d7149b2fb8014839035f5a",bookSignature:"Eng Hwa Yap",publishedDate:"January 18th 2017",coverURL:"https://cdn.intechopen.com/books/images_new/5361.jpg",numberOfDownloads:22660,numberOfWosCitations:28,numberOfCrossrefCitations:28,numberOfCrossrefCitationsByBook:2,numberOfDimensionsCitations:52,numberOfDimensionsCitationsByBook:2,hasAltmetrics:1,numberOfTotalCitations:108,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"February 29th 2016",dateEndSecondStepPublish:"March 21st 2016",dateEndThirdStepPublish:"June 25th 2016",dateEndFourthStepPublish:"September 23rd 2016",dateEndFifthStepPublish:"October 23rd 2016",currentStepOfPublishingProcess:5,indexedIn:"1,2,3,4,5,6,7",editedByType:"Edited by",kuFlag:!1,featuredMarkup:null,editors:[{id:"185577",title:"Associate Prof.",name:"Eng Hwa",middleName:null,surname:"Yap",slug:"eng-hwa-yap",fullName:"Eng Hwa Yap",profilePictureURL:"https://mts.intechopen.com/storage/users/185577/images/system/185577.jpeg",biography:"In the Faculty of Transdisciplinary Innovation, Eng Hwa Yap is\nthe Acting Associate Dean: Teaching and Learning (Operations).\nHe also leads the Bachelor of Technology and Innovation (BTi)\nas the Course Director. His research at the university focuses\non curated multidisciplinary and mixed approaches of enquiry\nto understand and respond to complex problems surrounding\ntechnology, environmental sustainability, and future energy\nsystems. In his research, Eng Hwa uses methods centering on systems thinking to\ninvestigate emerging and contemporary issues in technologies and their interaction\nwith society, the environment, economics, and policy.",institutionString:"University of Technology Sydney",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"0",totalChapterViews:"0",totalEditedBooks:"2",institution:{name:"University of Technology Sydney",institutionURL:null,country:{name:"Australia"}}}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,coeditorOne:null,coeditorTwo:null,coeditorThree:null,coeditorFour:null,coeditorFive:null,topics:[{id:"764",title:"Design Engineering",slug:"engineering-energy-engineering-design-engineering"}],chapters:[{id:"53600",title:"Incorporating Sustainable Development Principles into Building Design",doi:"10.5772/66825",slug:"incorporating-sustainable-development-principles-into-building-design",totalDownloads:2087,totalCrossrefCites:0,totalDimensionsCites:1,hasAltmetrics:0,abstract:"The main aim of this research is to utilise the focus of sustainable design to compare the material and subsequent environmental impacts of multi‐storey structures situated in Australia. The structure types under investigation were characterised by post‐tensioned and conventionally reinforced floor and roof flat plate slab systems. The foundation designs are undertaken for isolated spread footings on 32 structural model types with constant external dimensions which were composed of floor and roof slabs of varying concrete strength, span length and construction method, with all footing designs providing equivalent structural performance. The results from this study have reinforced the evidence that post‐tensioned construction can have significant effects in reducing material requirements and provide increased structural and environmental efficiency. Through reducing the frame mass, the footing systems were able to be designed using significantly less embodied energy when compared to the reinforced concrete structures. It is also noted that further investigation in the foundational requirements of these models is warranted, with the need to investigate the use of mat foundations for cases where isolated spread footings have required more than 50% of the structural plan area and for the footings that have required excessively thick sections to resist large shearing actions for larger spanned cases at 10 and 13.33 m.",signatures:"Jeung-Hwan Doh and Dane Miller",downloadPdfUrl:"/chapter/pdf-download/53600",previewPdfUrl:"/chapter/pdf-preview/53600",authors:[{id:"186139",title:"Dr.",name:"Jeung-Hwan",surname:"Doh",slug:"jeung-hwan-doh",fullName:"Jeung-Hwan Doh"},{id:"186949",title:"Dr.",name:"Dane",surname:"Miller",slug:"dane-miller",fullName:"Dane Miller"},{id:"374321",title:"Dr.",name:"Jeung-Hwan",surname:"Doh",slug:"jeung-hwan-doh",fullName:"Jeung-Hwan Doh"},{id:"374322",title:"Dr.",name:"Dane",surname:"Miller",slug:"dane-miller",fullName:"Dane Miller"}],corrections:null},{id:"52745",title:"Daylighting is More than an Energy Saving Issue",doi:"10.5772/65866",slug:"daylighting-is-more-than-an-energy-saving-issue",totalDownloads:1457,totalCrossrefCites:1,totalDimensionsCites:1,hasAltmetrics:1,abstract:"The focus in this chapter is to find adequate solutions to optimize daylighting of buildings. The climatic context is limited to Nordic European countries. As the knowledge of the positive impact of daylight on the human health increases and people spend most of their lives in buildings, the main objective is to create interiors with an optimum level of daylight for humans in a way which may also contribute to energy savings.",signatures:"Barbara Szybinska Matusiak",downloadPdfUrl:"/chapter/pdf-download/52745",previewPdfUrl:"/chapter/pdf-preview/52745",authors:[{id:"186233",title:"Prof.",name:"Barbara",surname:"Matusiak",slug:"barbara-matusiak",fullName:"Barbara Matusiak"},{id:"372328",title:"Dr.",name:"Barbara Szybinska",surname:"Matusiak",slug:"barbara-szybinska-matusiak",fullName:"Barbara Szybinska Matusiak"}],corrections:null},{id:"53060",title:"Energy Efficiency of Lightweight Steel-Framed Buildings",doi:"10.5772/66136",slug:"energy-efficiency-of-lightweight-steel-framed-buildings",totalDownloads:2807,totalCrossrefCites:1,totalDimensionsCites:7,hasAltmetrics:0,abstract:"The market share of lightweight steel‐framed (LSF) construction system has grown over the last decades, mainly in low‐rise residential buildings, due to its advantages such as having small weight with high mechanical strength; reduced disruption on‐site and speed of construction; great potential for recycling and reuse; high architectural flexibility for retrofitting purposes; easy prefabrication, allowing modular construction; economy in transportation and handling; superior quality given off‐site manufacture control; and excellent stability of shape in case of humidity and resistance to insect damage. However, given the high thermal conductivity of steel and the lightness of this type of construction, it may also have some drawbacks if not well designed and executed. Therefore, special attention should be given to the LSF building envelope in order to minimize thermal bridges. Moreover, given the usual reduced thermal mass, several strategies could be implemented to increase thermal inertia, consequently reduce indoor temperature fluctuations, enhance the occupants comfort and increase energy efficiency. In this chapter an overview of the main features related to the thermal behaviour and energy efficiency of LSF buildings is provided alongside some related case studies.",signatures:"Paulo Santos",downloadPdfUrl:"/chapter/pdf-download/53060",previewPdfUrl:"/chapter/pdf-preview/53060",authors:[{id:"186441",title:"Prof.",name:"Paulo",surname:"Santos",slug:"paulo-santos",fullName:"Paulo Santos"},{id:"372973",title:"Dr.",name:"Paulo",surname:"Santos",slug:"paulo-santos",fullName:"Paulo Santos"}],corrections:null},{id:"53729",title:"Building Typology Comparison Between Courtyard and Atrium Buildings: A Study of Thermal Comfort and Energy Performance Factors in Different Climate Zones",doi:"10.5772/66669",slug:"building-typology-comparison-between-courtyard-and-atrium-buildings-a-study-of-thermal-comfort-and-e",totalDownloads:2153,totalCrossrefCites:0,totalDimensionsCites:3,hasAltmetrics:0,abstract:"The aim of the study in this chapter is to investigate performances shown by courtyard buildings, used widely both as microclimate regulators and as city-wide climate stabilizers especially in the hot-dry climate regions. Furthermore, this study examines atrium buildings having an increasing usage rate in recent years and the presence of comfort problems in particular which have not been resolved for different climate regions. Wind velocity measurements are performed in 36 different points determined in X and Y directions and 17 different points in the Z dimension on the outside of the courtyard considered in this study. In addition, both atrium building typology model and courtyard building typology model are obtained by taking the average courtyard dimensions seen in many regions; by covering open space courtyard section of the geometry with a transparent glass, atrium and courtyard typologies can be obtained. Furthermore, thermal comfort states and energy performances of these two different building typologies in interior courtyard and in building internal volumes for hot-dry, hot-humid and cold climate region conditions as well as the effect of solar radiation values exposing the building surfaces and solar movements during the day on the thermal performance on the building are analysed with CFD FloEFD and Star CCM+ software.",signatures:"Enes Yasa",downloadPdfUrl:"/chapter/pdf-download/53729",previewPdfUrl:"/chapter/pdf-preview/53729",authors:[{id:"185967",title:"Associate Prof.",name:"Enes",surname:"Yasa",slug:"enes-yasa",fullName:"Enes Yasa"},{id:"374708",title:"Dr.",name:"Enes",surname:"Yasa",slug:"enes-yasa",fullName:"Enes Yasa"}],corrections:null},{id:"53557",title:"Energy-Efficient Building Design in the Context of Building Life Cycle",doi:"10.5772/66670",slug:"energy-efficient-building-design-in-the-context-of-building-life-cycle",totalDownloads:5553,totalCrossrefCites:19,totalDimensionsCites:30,hasAltmetrics:1,abstract:"Energy is one of the most important catalysts in wealth generation, economic growth, and social development in all countries. Buildings have a significant share in total energy consumed globally; therefore, they have a profound impact upon the environment. Energy is used in every stage of building life cycle (these stages are choice of locality, architectural design, structural systems and material selection, building construction, usage and maintenance, demolition, reuse-regain-recycle, and waste disposal). According to World Watch Institute data, buildings are responsible for the annual consumption of 40% of the world's energy. Energy consumption of buildings can be reduced significantly in every stage of a building life cycle. This study investigated the energy-efficient methods in building life cycle. In this context, we give information about the life cycle of building and explain energy-efficient guiding principles in life cycle stages.",signatures:"Izzet Yüksek and Tülay Tikansak Karadayi",downloadPdfUrl:"/chapter/pdf-download/53557",previewPdfUrl:"/chapter/pdf-preview/53557",authors:[{id:"186397",title:"Dr.",name:"İzzet",surname:"Yüksek",slug:"izzet-yuksek",fullName:"İzzet Yüksek"},{id:"186398",title:"Prof.",name:"Tülay",surname:"Tıkansak Karadayı",slug:"tulay-tikansak-karadayi",fullName:"Tülay Tıkansak Karadayı"},{id:"374197",title:"Dr.",name:"Izzet",surname:"Yüksek",slug:"izzet-yuksek",fullName:"Izzet Yüksek"},{id:"374198",title:"Dr.",name:"Tülay Tikansak",surname:"Karadayi",slug:"tulay-tikansak-karadayi",fullName:"Tülay Tikansak Karadayi"}],corrections:null},{id:"51988",title:"Energy Efficiency in Manufacturing Facilities: Assessment, Analysis and Implementation",doi:"10.5772/64902",slug:"energy-efficiency-in-manufacturing-facilities-assessment-analysis-and-implementation",totalDownloads:2576,totalCrossrefCites:5,totalDimensionsCites:7,hasAltmetrics:0,abstract:"Manufacturing facilities are one among the largest consumers of energy. Efforts to improve energy efficiency are an increasing concern for many manufacturing facility engineering managers. This can be achieved by evaluating energy end uses (e.g., lighting, processing equipment, and heating, air conditioning, and ventilation (HVAC) systems), and by implementing measures to reduce the total amount of energy consumed for one or more of the end uses. Throughout the 40 years of its existence, the US Department of Energy’s Industrial Assessment Center program has developed an array of techniques to improve energy efficiency in industrial facilities. This chapter discusses recommended assessment procedures and observed energy-saving opportunities for some of the most common industrial energy systems. These systems include lighting, compressed air, boilers and steam systems, manufacturing process heating, HVAC, pumps, and fans. Implementation of these assessment recommendations has been demonstrated to increase efficiency and to thus reduce energy consumption and associated costs. While every manufacturing facility is different, and their component industrial energy systems equally unique, this chapter presents a set of analytical guidelines that can be used as a template by engineering practitioners to evaluate their facility energy use and implement subsequent energy conservation measures.",signatures:"Gary P. Moynihan and Frank L. Barringer",downloadPdfUrl:"/chapter/pdf-download/51988",previewPdfUrl:"/chapter/pdf-preview/51988",authors:[{id:"16974",title:"Dr.",name:"Gary",surname:"Moynihan",slug:"gary-moynihan",fullName:"Gary Moynihan"},{id:"193958",title:"Mr.",name:"Frank",surname:"Barringer",slug:"frank-barringer",fullName:"Frank Barringer"},{id:"371713",title:"Dr.",name:"Frank L.",surname:"Barringer",slug:"frank-l.-barringer",fullName:"Frank L. Barringer"}],corrections:null},{id:"53147",title:"Energy Efficiency of Hydronic Heating System in Retrofitted Buildings",doi:"10.5772/66364",slug:"energy-efficiency-of-hydronic-heating-system-in-retrofitted-buildings",totalDownloads:1666,totalCrossrefCites:0,totalDimensionsCites:1,hasAltmetrics:0,abstract:"Since central-based heating systems with radiators are among the most widely used heating systems in Europe, retrofit measures should include heat distribution and heat emission elements. One of the most cost-effective measures for heating systems is the replacement of the heat generator and circulating pump while preserving the distribution system and heat emitters. Reducing the heating demand results in an oversized existing heating system and thus enables a reduction in flow rates and supply temperatures. These steps should be taken to enhance the efficiency of the heating system without affecting the level of thermal comfort. This chapter focuses on the issue of energy efficiency in retrofitted buildings by optimizing the existing heating systems. The heating equipment is considered as one system, and it is not intended to improve only individual component efficiency. The optimization goal is to achieve a recommended or prescribed thermal comfort level with minimal energy use.",signatures:"Matjaž Prek",downloadPdfUrl:"/chapter/pdf-download/53147",previewPdfUrl:"/chapter/pdf-preview/53147",authors:[{id:"144302",title:"Dr.",name:"Matjaz",surname:"Prek",slug:"matjaz-prek",fullName:"Matjaz Prek"},{id:"373192",title:"Dr.",name:"Matjaž",surname:"Prek",slug:"matjaz-prek",fullName:"Matjaž Prek"}],corrections:null},{id:"53738",title:"Managing Energy Demand in Buildings through Appropriate Equipment Specification and Use",doi:"10.5772/66363",slug:"managing-energy-demand-in-buildings-through-appropriate-equipment-specification-and-use",totalDownloads:2164,totalCrossrefCites:1,totalDimensionsCites:1,hasAltmetrics:0,abstract:"The high demand for electrical energy in virtually all human endeavour has engendered the continuous rapid growth of electricity production, transmission and distribution worldwide. Every habitable building structure usually requires electrical appliances and mechanical systems such that the cost of electrical and mechanical installation in a building is generally between 10% and 35% of the total construction cost. This chapter examines the equipment used for electrical, mechanical and lighting systems in contemporary buildings in Cape Town, South Africa, towards determining those materials and equipment aiding energy efficiency in these buildings. The research employs a multiple case study approach, consisting of recently completed buildings. The study established that the equipment used in these contemporary buildings to effect a reduction in energy consumption are compliant with the main specifications and policies guiding energy efficiency in buildings in South Africa and that owners of the Case Study buildings obtained a significant reduction in power consumption as a result of the installation of the identified equipment. Based on these findings, the study concludes that a building that is compliant with energy efficient systems installation standards will experience a significant reduction in utility bills, and savings for commercial buildings and private property owners.",signatures:"Abimbola Olukemi Windapo",downloadPdfUrl:"/chapter/pdf-download/53738",previewPdfUrl:"/chapter/pdf-preview/53738",authors:[{id:"186236",title:"Prof.",name:"Abimbola Olukemi",surname:"Windapo",slug:"abimbola-olukemi-windapo",fullName:"Abimbola Olukemi Windapo"},{id:"374738",title:"Dr.",name:"Abimbola Olukemi",surname:"Windapo",slug:"abimbola-olukemi-windapo",fullName:"Abimbola Olukemi Windapo"}],corrections:null},{id:"53183",title:"Towards Near Zero Energy Home",doi:"10.5772/66224",slug:"towards-near-zero-energy-home",totalDownloads:2197,totalCrossrefCites:1,totalDimensionsCites:1,hasAltmetrics:0,abstract:"In the context of building design, as investment in the built-environment continues to grow, the requirement to deliver low-energy buildings will become ever more pressing as natural resources dwindle and consumer energy costs increase. Energy efficiency awareness and regulations continue to rise in the Gulf Cooperation Council (GCC) countries but the majority of building stock of which the larger share in energy consumption has not been designed for energy efficiency. The design and construction of buildings in hot humid climates require high-energy consumption typically for air conditioning due to higher thermal loads. Regionally, there is a rising concern on the current rate of energy consumption due to air conditioning. The global sustainability assessment system (GSAS), a performance-based system raised the bar of efficient design by the development of stringent energy passive design benchmarks on the thermal cooling need in buildings. This chapter introduces the simulation measures undertaken to reduce the cooling need using a ‘showcase’ house or the ‘near Zero Energy Home’ (nZEH), which is currently under construction. The chapter presents and discusses the Be Lean, Be Clean and Be Green strategies that used to reduce the cooling demand by more than 80% and the overall energy consumption by 75%.",signatures:"Esam Elsarrag and Yousef Alhorr",downloadPdfUrl:"/chapter/pdf-download/53183",previewPdfUrl:"/chapter/pdf-preview/53183",authors:[{id:"62641",title:"Dr.",name:"Esam",surname:"Elsarrag",slug:"esam-elsarrag",fullName:"Esam Elsarrag"},{id:"186304",title:"Prof.",name:"Yousef",surname:"Alhorr",slug:"yousef-alhorr",fullName:"Yousef Alhorr"},{id:"373274",title:"Dr.",name:"Esam",surname:"Elsarrag",slug:"esam-elsarrag",fullName:"Esam Elsarrag"},{id:"373275",title:"Dr.",name:"Yousef",surname:"Alhorr",slug:"yousef-alhorr",fullName:"Yousef Alhorr"}],corrections:null}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"},subseries:null,tags:null},relatedBooks:[{type:"book",id:"7200",title:"Green Energy and Environment",subtitle:null,isOpenForSubmission:!1,hash:"72ad3cb35d7eb84855d6cb05c6e73897",slug:"green-energy-and-environment",bookSignature:"Eng Hwa Yap and Andrew Huey Ping Tan",coverURL:"https://cdn.intechopen.com/books/images_new/7200.jpg",editedByType:"Edited by",editors:[{id:"185577",title:"Associate Prof.",name:"Eng Hwa",surname:"Yap",slug:"eng-hwa-yap",fullName:"Eng Hwa Yap"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"8890",title:"Design Optimization of Wind Energy Conversion Systems with Applications",subtitle:null,isOpenForSubmission:!1,hash:"a2ce9419202c074e3aee8dff0d87326c",slug:"design-optimization-of-wind-energy-conversion-systems-with-applications",bookSignature:"Karam Y. Maalawi",coverURL:"https://cdn.intechopen.com/books/images_new/8890.jpg",editedByType:"Edited by",editors:[{id:"18593",title:"Prof.",name:"Karam",surname:"Maalawi",slug:"karam-maalawi",fullName:"Karam Maalawi"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"1591",title:"Infrared Spectroscopy",subtitle:"Materials Science, Engineering and Technology",isOpenForSubmission:!1,hash:"99b4b7b71a8caeb693ed762b40b017f4",slug:"infrared-spectroscopy-materials-science-engineering-and-technology",bookSignature:"Theophile Theophanides",coverURL:"https://cdn.intechopen.com/books/images_new/1591.jpg",editedByType:"Edited by",editors:[{id:"37194",title:"Dr.",name:"Theophile",surname:"Theophanides",slug:"theophile-theophanides",fullName:"Theophile Theophanides"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"3161",title:"Frontiers in Guided Wave Optics and Optoelectronics",subtitle:null,isOpenForSubmission:!1,hash:"deb44e9c99f82bbce1083abea743146c",slug:"frontiers-in-guided-wave-optics-and-optoelectronics",bookSignature:"Bishnu Pal",coverURL:"https://cdn.intechopen.com/books/images_new/3161.jpg",editedByType:"Edited by",editors:[{id:"4782",title:"Prof.",name:"Bishnu",surname:"Pal",slug:"bishnu-pal",fullName:"Bishnu Pal"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"371",title:"Abiotic Stress in Plants",subtitle:"Mechanisms and Adaptations",isOpenForSubmission:!1,hash:"588466f487e307619849d72389178a74",slug:"abiotic-stress-in-plants-mechanisms-and-adaptations",bookSignature:"Arun Shanker and B. Venkateswarlu",coverURL:"https://cdn.intechopen.com/books/images_new/371.jpg",editedByType:"Edited by",editors:[{id:"58592",title:"Dr.",name:"Arun",surname:"Shanker",slug:"arun-shanker",fullName:"Arun Shanker"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"3092",title:"Anopheles mosquitoes",subtitle:"New insights into malaria vectors",isOpenForSubmission:!1,hash:"c9e622485316d5e296288bf24d2b0d64",slug:"anopheles-mosquitoes-new-insights-into-malaria-vectors",bookSignature:"Sylvie Manguin",coverURL:"https://cdn.intechopen.com/books/images_new/3092.jpg",editedByType:"Edited by",editors:[{id:"50017",title:"Prof.",name:"Sylvie",surname:"Manguin",slug:"sylvie-manguin",fullName:"Sylvie Manguin"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"72",title:"Ionic Liquids",subtitle:"Theory, Properties, New Approaches",isOpenForSubmission:!1,hash:"d94ffa3cfa10505e3b1d676d46fcd3f5",slug:"ionic-liquids-theory-properties-new-approaches",bookSignature:"Alexander Kokorin",coverURL:"https://cdn.intechopen.com/books/images_new/72.jpg",editedByType:"Edited by",editors:[{id:"19816",title:"Prof.",name:"Alexander",surname:"Kokorin",slug:"alexander-kokorin",fullName:"Alexander Kokorin"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"2270",title:"Fourier Transform",subtitle:"Materials Analysis",isOpenForSubmission:!1,hash:"5e094b066da527193e878e160b4772af",slug:"fourier-transform-materials-analysis",bookSignature:"Salih Mohammed Salih",coverURL:"https://cdn.intechopen.com/books/images_new/2270.jpg",editedByType:"Edited by",editors:[{id:"111691",title:"Dr.Ing.",name:"Salih",surname:"Salih",slug:"salih-salih",fullName:"Salih Salih"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"117",title:"Artificial Neural Networks",subtitle:"Methodological Advances and Biomedical Applications",isOpenForSubmission:!1,hash:null,slug:"artificial-neural-networks-methodological-advances-and-biomedical-applications",bookSignature:"Kenji Suzuki",coverURL:"https://cdn.intechopen.com/books/images_new/117.jpg",editedByType:"Edited by",editors:[{id:"3095",title:"Prof.",name:"Kenji",surname:"Suzuki",slug:"kenji-suzuki",fullName:"Kenji Suzuki"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"3828",title:"Application of Nanotechnology in Drug Delivery",subtitle:null,isOpenForSubmission:!1,hash:"51a27e7adbfafcfedb6e9683f209cba4",slug:"application-of-nanotechnology-in-drug-delivery",bookSignature:"Ali Demir Sezer",coverURL:"https://cdn.intechopen.com/books/images_new/3828.jpg",editedByType:"Edited by",editors:[{id:"62389",title:"PhD.",name:"Ali Demir",surname:"Sezer",slug:"ali-demir-sezer",fullName:"Ali Demir Sezer"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}}],ofsBooks:[]},correction:{item:{id:"79356",slug:"addendum-an-overview-of-pet-radiopharmaceuticals-in-clinical-use-regulatory-quality-and-pharmacopeia",title:"Addendum - An Overview of PET Radiopharmaceuticals in Clinical Use: Regulatory, Quality and Pharmacopeia Monographs of the United States and Europe",doi:null,correctionPDFUrl:"https://cdn.intechopen.com/pdfs/66068.pdf",downloadPdfUrl:"/chapter/pdf-download/66068",previewPdfUrl:"/chapter/pdf-preview/66068",totalDownloads:null,totalCrossrefCites:null,bibtexUrl:"/chapter/bibtex/66068",risUrl:"/chapter/ris/66068",chapter:{id:"62269",slug:"an-overview-of-pet-radiopharmaceuticals-in-clinical-use-regulatory-quality-and-pharmacopeia-monograp",signatures:"Ya-Yao Huang",dateSubmitted:"February 25th 2018",dateReviewed:"May 31st 2018",datePrePublished:"November 5th 2018",datePublished:"July 24th 2019",book:{id:"7373",title:"Nuclear Medicine Physics",subtitle:null,fullTitle:"Nuclear Medicine Physics",slug:"nuclear-medicine-physics",publishedDate:"July 24th 2019",bookSignature:"Aamir Shahzad and Sajid Bashir",coverURL:"https://cdn.intechopen.com/books/images_new/7373.jpg",licenceType:"CC BY 3.0",editedByType:"Edited by",editors:[{id:"288354",title:"Dr.",name:"Aamir",middleName:null,surname:"Shahzad",slug:"aamir-shahzad",fullName:"Aamir Shahzad"}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"}},authors:[{id:"247754",title:"Prof.",name:"Ya-Yao",middleName:null,surname:"Huang",fullName:"Ya-Yao Huang",slug:"ya-yao-huang",email:"careyyh@ntuh.gov.tw",position:null,institution:{name:"National Taiwan University Hospital",institutionURL:null,country:{name:"Taiwan"}}}]}},chapter:{id:"62269",slug:"an-overview-of-pet-radiopharmaceuticals-in-clinical-use-regulatory-quality-and-pharmacopeia-monograp",signatures:"Ya-Yao Huang",dateSubmitted:"February 25th 2018",dateReviewed:"May 31st 2018",datePrePublished:"November 5th 2018",datePublished:"July 24th 2019",book:{id:"7373",title:"Nuclear Medicine Physics",subtitle:null,fullTitle:"Nuclear Medicine Physics",slug:"nuclear-medicine-physics",publishedDate:"July 24th 2019",bookSignature:"Aamir Shahzad and Sajid Bashir",coverURL:"https://cdn.intechopen.com/books/images_new/7373.jpg",licenceType:"CC BY 3.0",editedByType:"Edited by",editors:[{id:"288354",title:"Dr.",name:"Aamir",middleName:null,surname:"Shahzad",slug:"aamir-shahzad",fullName:"Aamir Shahzad"}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"}},authors:[{id:"247754",title:"Prof.",name:"Ya-Yao",middleName:null,surname:"Huang",fullName:"Ya-Yao Huang",slug:"ya-yao-huang",email:"careyyh@ntuh.gov.tw",position:null,institution:{name:"National Taiwan University Hospital",institutionURL:null,country:{name:"Taiwan"}}}]},book:{id:"7373",title:"Nuclear Medicine Physics",subtitle:null,fullTitle:"Nuclear Medicine Physics",slug:"nuclear-medicine-physics",publishedDate:"July 24th 2019",bookSignature:"Aamir Shahzad and Sajid Bashir",coverURL:"https://cdn.intechopen.com/books/images_new/7373.jpg",licenceType:"CC BY 3.0",editedByType:"Edited by",editors:[{id:"288354",title:"Dr.",name:"Aamir",middleName:null,surname:"Shahzad",slug:"aamir-shahzad",fullName:"Aamir Shahzad"}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"}}},ofsBook:{item:{type:"book",id:"11699",leadTitle:null,title:"Neonatal Surgery",subtitle:null,reviewType:"peer-reviewed",abstract:"This book will be a self-contained collection of scholarly papers targeting an audience of practicing researchers, academics, PhD students and other scientists. The contents of the book will be written by multiple authors and edited by experts in the field.",isbn:null,printIsbn:null,pdfIsbn:null,doi:null,price:0,priceEur:0,priceUsd:0,slug:null,numberOfPages:0,isOpenForSubmission:!0,isSalesforceBook:!1,isNomenclature:!1,hash:"e52adaee8e54f51c2ba4972daeb410f7",bookSignature:"",publishedDate:null,coverURL:"https://cdn.intechopen.com/books/images_new/11699.jpg",keywords:null,numberOfDownloads:null,numberOfWosCitations:0,numberOfCrossrefCitations:null,numberOfDimensionsCitations:null,numberOfTotalCitations:null,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"September 30th 2021",dateEndSecondStepPublish:"October 21st 2021",dateEndThirdStepPublish:"December 20th 2021",dateEndFourthStepPublish:"March 10th 2022",dateEndFifthStepPublish:"May 9th 2022",dateConfirmationOfParticipation:null,remainingDaysToSecondStep:"10 months",secondStepPassed:!0,areRegistrationsClosed:!0,currentStepOfPublishingProcess:1,editedByType:null,kuFlag:!1,biosketch:null,coeditorOneBiosketch:null,coeditorTwoBiosketch:null,coeditorThreeBiosketch:null,coeditorFourBiosketch:null,coeditorFiveBiosketch:null,editors:null,coeditorOne:null,coeditorTwo:null,coeditorThree:null,coeditorFour:null,coeditorFive:null,topics:[{id:"16",title:"Medicine",slug:"medicine"}],chapters:null,productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"},personalPublishingAssistant:null},relatedBooks:[{type:"book",id:"6550",title:"Cohort Studies in Health Sciences",subtitle:null,isOpenForSubmission:!1,hash:"01df5aba4fff1a84b37a2fdafa809660",slug:"cohort-studies-in-health-sciences",bookSignature:"R. Mauricio Barría",coverURL:"https://cdn.intechopen.com/books/images_new/6550.jpg",editedByType:"Edited by",editors:[{id:"88861",title:"Dr.",name:"R. Mauricio",surname:"Barría",slug:"r.-mauricio-barria",fullName:"R. Mauricio Barría"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"9500",title:"Recent Advances in Bone Tumours and Osteoarthritis",subtitle:null,isOpenForSubmission:!1,hash:"ea4ec0d6ee01b88e264178886e3210ed",slug:"recent-advances-in-bone-tumours-and-osteoarthritis",bookSignature:"Hiran Amarasekera",coverURL:"https://cdn.intechopen.com/books/images_new/9500.jpg",editedByType:"Edited by",editors:[{id:"67634",title:"Dr.",name:"Hiran",surname:"Amarasekera",slug:"hiran-amarasekera",fullName:"Hiran Amarasekera"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"1591",title:"Infrared Spectroscopy",subtitle:"Materials Science, Engineering and Technology",isOpenForSubmission:!1,hash:"99b4b7b71a8caeb693ed762b40b017f4",slug:"infrared-spectroscopy-materials-science-engineering-and-technology",bookSignature:"Theophile Theophanides",coverURL:"https://cdn.intechopen.com/books/images_new/1591.jpg",editedByType:"Edited by",editors:[{id:"37194",title:"Dr.",name:"Theophile",surname:"Theophanides",slug:"theophile-theophanides",fullName:"Theophile Theophanides"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"3161",title:"Frontiers in Guided Wave Optics and Optoelectronics",subtitle:null,isOpenForSubmission:!1,hash:"deb44e9c99f82bbce1083abea743146c",slug:"frontiers-in-guided-wave-optics-and-optoelectronics",bookSignature:"Bishnu Pal",coverURL:"https://cdn.intechopen.com/books/images_new/3161.jpg",editedByType:"Edited by",editors:[{id:"4782",title:"Prof.",name:"Bishnu",surname:"Pal",slug:"bishnu-pal",fullName:"Bishnu Pal"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"371",title:"Abiotic Stress in Plants",subtitle:"Mechanisms and Adaptations",isOpenForSubmission:!1,hash:"588466f487e307619849d72389178a74",slug:"abiotic-stress-in-plants-mechanisms-and-adaptations",bookSignature:"Arun Shanker and B. Venkateswarlu",coverURL:"https://cdn.intechopen.com/books/images_new/371.jpg",editedByType:"Edited by",editors:[{id:"58592",title:"Dr.",name:"Arun",surname:"Shanker",slug:"arun-shanker",fullName:"Arun Shanker"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"3092",title:"Anopheles mosquitoes",subtitle:"New insights into malaria vectors",isOpenForSubmission:!1,hash:"c9e622485316d5e296288bf24d2b0d64",slug:"anopheles-mosquitoes-new-insights-into-malaria-vectors",bookSignature:"Sylvie Manguin",coverURL:"https://cdn.intechopen.com/books/images_new/3092.jpg",editedByType:"Edited by",editors:[{id:"50017",title:"Prof.",name:"Sylvie",surname:"Manguin",slug:"sylvie-manguin",fullName:"Sylvie Manguin"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"72",title:"Ionic Liquids",subtitle:"Theory, Properties, New Approaches",isOpenForSubmission:!1,hash:"d94ffa3cfa10505e3b1d676d46fcd3f5",slug:"ionic-liquids-theory-properties-new-approaches",bookSignature:"Alexander Kokorin",coverURL:"https://cdn.intechopen.com/books/images_new/72.jpg",editedByType:"Edited by",editors:[{id:"19816",title:"Prof.",name:"Alexander",surname:"Kokorin",slug:"alexander-kokorin",fullName:"Alexander Kokorin"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"2270",title:"Fourier Transform",subtitle:"Materials Analysis",isOpenForSubmission:!1,hash:"5e094b066da527193e878e160b4772af",slug:"fourier-transform-materials-analysis",bookSignature:"Salih Mohammed Salih",coverURL:"https://cdn.intechopen.com/books/images_new/2270.jpg",editedByType:"Edited by",editors:[{id:"111691",title:"Dr.Ing.",name:"Salih",surname:"Salih",slug:"salih-salih",fullName:"Salih Salih"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"117",title:"Artificial Neural Networks",subtitle:"Methodological Advances and Biomedical Applications",isOpenForSubmission:!1,hash:null,slug:"artificial-neural-networks-methodological-advances-and-biomedical-applications",bookSignature:"Kenji Suzuki",coverURL:"https://cdn.intechopen.com/books/images_new/117.jpg",editedByType:"Edited by",editors:[{id:"3095",title:"Prof.",name:"Kenji",surname:"Suzuki",slug:"kenji-suzuki",fullName:"Kenji Suzuki"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"3828",title:"Application of Nanotechnology in Drug Delivery",subtitle:null,isOpenForSubmission:!1,hash:"51a27e7adbfafcfedb6e9683f209cba4",slug:"application-of-nanotechnology-in-drug-delivery",bookSignature:"Ali Demir Sezer",coverURL:"https://cdn.intechopen.com/books/images_new/3828.jpg",editedByType:"Edited by",editors:[{id:"62389",title:"PhD.",name:"Ali Demir",surname:"Sezer",slug:"ali-demir-sezer",fullName:"Ali Demir Sezer"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}}]},chapter:{item:{type:"chapter",id:"73207",title:"Lipoproteomics: Methodologies and Analysis of Lipoprotein-Associated Proteins along with the Drug Intervention",doi:"10.5772/intechopen.93634",slug:"lipoproteomics-methodologies-and-analysis-of-lipoprotein-associated-proteins-along-with-the-drug-int",body:'The emerging high-throughput omics such as genomics, transcriptomics, proteomics, and metabolomics have been used in the search of new biomarkers in several diseases. Proteomic analyses or metabolomics and lipidomics as well as complementary technologies such as mass spectrometry (MS) (i.e., LC-MS-MS and MALDI-TOF/TOF), nuclear magnetic resonance spectroscopy, and other omics technologies, are being widely used in the search of new sources of markers, candidates for vaccine, and alteration of expression patterns in response to environmental changes and signaling pathways in different diseases. The search for proteins in the dynamic system of a proteome requires various proteomics approaches and the use of proteomics is crucial for the early disease diagnosis, prognosis, and to monitor the disease development. The proteomics is essential for the understanding of complex biochemical processes, and the high-throughput proteomics increases the depth of proteome coverage.
Lipoproteins are macromolecular complex particles of lipids and proteins, which are related to the extracellular transport of lipids in many organisms [1]. Besides, lipoproteins have been also implicated as important host defense mediators and in the initiation of immune responses [2, 3, 4]. Lipid content has long been recognized as a critical factor in lipoprotein metabolism that acts as an important determinant of human health [5]. However, in last years, apolipoproteins and lipoprotein-associated proteins have been taken on relevance regarding lipoprotein metabolism since they serve as a frame for their assembly, maintain their structure, and interact with the membrane receptors and enzymes. Therefore, these proteins could be crucial in the identification of biomarkers related to diseases due to the fact that they are being studied under a global approach, denominated lipoproteome [6, 7].
Lipoproteins are complex protein particles of an amphipathic nature, structurally are formed by an outer layer of phospholipids, free cholesterol, and apolipoproteins, and inside contain a nucleus of cholesterol esters and triglycerides [8]. When the lipoprotein complex is formed, the orientation of the hydrophilic proportions is toward the outside and the lipophilic proportions toward the interior; this structural characteristic allows the complex to have the ability to emulsify fats in extracellular fluids [8]. Based on their density defined by the protein to lipids ratios, lipoproteins are grouped into six classes: chylomicrons, very low density lipoproteins (VLDL), intermediate density lipoproteins (IDL), low density lipoproteins (LDL), high density lipoproteins (HDL), and lipoprotein (a) [Lp (a)] [8, 9].
Triglycerides, derived from dietary fat absorption by the small intestine, are carried by the chylomicrons into blood. After triglyceride digestion to free fatty acids (FFA) by lipases in the peripheral tissues, the size of these particles is reduced, which leads to the formation of chylomicron remnants. These latter are cleared by liver uptake via LDL receptor-related protein (LRP). To synthesize VLDL, newly synthesized triglyceride and cholesterol by the liver are incorporated into chylomicron remnants [8, 10]. These large triglyceride-rich VLDLs are released to the circulation and travel to peripheral tissues, where the lipase digestion of triglycerides occurs resulting in the IDL formation (VLDL remnants). These IDL particles can be either cleared from the circulation by the liver in a similar manner to that described for chylomicrons remnants or can be digested by hepatic lipase to generate cholesterol-rich LDL particles, which is taken up by the peripheral tissues via LDL receptor to supply their cholesterol requirements [10].
The HDL lipoproteins have the highest relative density as compared to other lipoproteins despite being the smallest in size and heterogeneous in terms of composition. These HDL particles play an important role in the transport of reverse cholesterol as a carrier in the movement of cholesterol from peripheral tissues back into the liver [11]. The liver and intestine, practically in response to the lipolysis of triglyceride-rich lipoproteins, synthesize and secrete the nascent discoid HDLs that consist primarily of phospholipids and free cholesterol. Then, these particles reach the plasma where additional exchangeable apolipoproteins are picked up and excess free cholesterol is removed from both extrahepatic cells and other circulating lipoproteins, forming mature spherical HDL particles. Finally, these cholesterol-rich HDL particles can be delivered back to the liver [10, 12].
The protein cargo related to lipoproteins consists of apolipoproteins and lipoprotein-associated proteins. These proteins play key roles in lipoprotein metabolism such as structural component, enzyme interaction, and receptors recognition, among other functions [7]. Changes in the quantity and type of these proteins could be involved in the outcome of diseases related to lipid metabolism. Therefore, the proteomic analysis of lipoproteins is an important notion for the present revision.
The apolipoproteins can be classified as integral or peripheral, either they act as constituent components of the plasma membrane of the lipoproteins or they bind to the membrane and can be exchanged between one complex and another, respectively. Apolipoproteins are distributed throughout all lipoproteins and subfractions, with the proportion of them varying in each family. These variations in the amount of proteins present in the lipoprotein families in their membrane gives them their capacity to interact with different tissues [7, 10].
Within the apolipoprotein A (ApoA) group is the apolipoprotein A-I (ApoA-I), the main protein component of HDL, which plays multiple roles in the transport of cholesterol, in addition to having been linked to the regulation of some functions of the inflammatory and immune response [13]. ApoA-II, also found in HDL, acts as an enzymatic inhibitor of lipoproteins and liver lipases. The apolipoproteins are capable of binding to each other to modify the interaction of the lipoprotein complex, as is the case with apoA-I and apoA-II in LDL [10].
ApoA-IV is mainly synthesized in the small intestine where it is attached by enterocytes to the chylomicrons and secreted during a high-fat meal intake. ApoA-IV is associated with HDL and chylomicron remnants in circulation, but a significant portion is free [14]. On the other hand, ApoA-V participates in the regulation of triglyceride levels in plasma [15]. ApoA-V is expressed only in the liver and circulates at low concentrations. Despite this, apoA-V can be recovered in association with plasma lipoproteins [16].
The apolipoprotein B (ApoB), the main protein component of chylomicrons, LDL, VLDL, IDL, and Lp (a), is encoded by a single gene that gives rises to two isoforms: ApoB-48 and ApoB-100. ApoB-48 is produced exclusively in the intestine and is the major structural protein of chylomicrons and chylomicron remnants. ApoB-100 is expressed in the liver and is found only in VLDL, IDL, LDL, and Lp (a). ApoB-containing lipoproteins are characterized by a spherical shape and contain one single apoB-48 or apoB-100 molecule per lipoprotein [17].
Apolipoprotein C (ApoC) works as an inhibitor of certain processes and modulators of catabolism. ApoC is mainly synthesized in the liver and easily transferred between lipoprotein particles and therefore are found associated with chylomicrons, VLDL, and HDL [10]. ApoC-I is responsible for inhibiting the binding of lipoprotein to its receptor as well as the function of the esterified cholesterol transfer protein (CETP). ApoC-II is an essential cofactor of the lipoprotein lipase involved in triglyceride hydrolysis. Both apoC-III and apoC-IV inhibit triglyceride hydrolysis. In addition, apoC-III decreases the clearance of VLDL [10, 18].
Several tissues synthesize apolipoprotein E (ApoE), an exchangeable apolipoprotein associated with chylomicrons, chylomicron remnants, VLDL, IDL, and a subgroup of HDL particles, but the liver and intestine are the principal producers of circulating ApoE [10]. ApoE functions as a lipoprotein ligator with hepatocytes (clearance of apoE-containing lipoproteins) and peripheral cells related to the LDL receptor. ApoCs and apoEs are interchangeable between complexes during the conversion of VLDL to LDL. ApoEs may function as a lecithin-cholesterol acyltransferase (LCAT) activator and influence the activity of hepatic lipase and CETP [19, 20].
Proteins belonging to the lipocalins family such as apoD, apoM, the orosomucoid protein, and retinol-binding protein (RBP) interact with the surface receptors of the cells, intervening in the formation of macromolecular complexes. It has been described that apoD binds to apoA-II and apoB-100 by means of disulfide bridges; it is associated with LCAT, so it is involved in the transport of lipids in the blood system. ApoJ is involved in a wide variety of processes, acting as a chaperone protein and its main role is the inhibition of lipid transfer and is related to cell death. There are apoproteins such as apoF whose function is unknown; however, there is a theory of their role in abnormal lipid composition inhibiting CETP in the small and dense LDL particles (sdLDL) [21, 22].
In addition to apolipoproteins, lipoproteins require other proteins with specific activities to interact with the environment, which are denominated as lipoprotein-associated proteins. Between these proteins are included phospholipase A2, whose activity indicates the presence of sdLDL in plasma [23]. Serum amyloid A protein (SAA) is associated with several lipoproteins, especially in LDL [24]. Some proteins such as albumin and prenyl cysteine oxidase (PCYOX1) are responsible for protecting against oxidation by lipoproteins such as LDL and splitting the thioether bond of the prenyl cysteine generating H2O2, respectively. Also, proteomic studies have identified the apoL-I, PON1, and PAF-AH in small amounts linked to the complexes [25, 26].
The function of many of the proteins that interact with lipoproteins is still unknown. The proteomic analyses have shown that the lipoprotein-associated proteins are involved in cardiovascular risk, immune system, inflammatory processes, among others [7, 26]. However, there is no guide for the analysis of lipoproteins at the proteomic level basically because it depends on the biological question to be investigated, and this determines the approach and the methods or tools to be used as well as the technological platform to perform it.
Before describing the methodologies used for lipoproteomic studies, it is important to mention some general characteristics of lipoprotein-associated proteins and the importance of the research question to establish a good methodological flow chart to address this question. We will start by mentioning that must first consider the lipoprotein obtention by using methods for separation, concentration, and protein stability due to the heterogeneity of these particles.
Diverse chromatographic techniques—prior to proteomic techniques—have been used for the separation of plasma lipoproteins as well as the protein content, such as capillary electrophoresis, size exclusion, cation exchange, gel filtration, fast protein liquid, among other chromatographic techniques [7, 27, 28, 29, 30, 31, 32].
The two-dimensional electrophoresis (2-DE) is one of the most usual methods that have been applied to separate the protein cargo related to lipoproteins. Although usually, the first-dimensional separation applied for the proteins in the 2-DE is on base of their charge (isoelectrofocusing), some lipoproteomic analyses have substituted this step by either one of the abovementioned methods to separate lipoproteins or electrophoresis on native gels, followed by SDS-PAGE, which could be denominated as gel-based lipoproteomics [27, 33, 34, 35].
Lipoprotein-associated proteins are not easy to study by proteomic methods, mainly the embedded in the lipoprotein membranes. These latter have rigid transmembrane domains that contain α-helices or β-barrels, which stabilize the protein by strong secondary structural characteristics and these regions can resist proteolytic digestion [6, 36]. Thus, the protein identification for lipoproteomes has been mainly performed by two methods: mass spectrometry and resonance magnetic nuclear.
Modern mass spectrometry (MS) techniques have allowed for thorough characterizations of the lipoproteins [37]. However, different experimental conditions have been reported to avoid contamination of the biological samples as well as selective and optimized methods to detect lipoproteins, usually liquid-phase separation techniques, prior to spectrometric techniques [36, 37]. Thus, different spectrometric experimentation conditions have been reported for the study of lipoproteins and apoproteins, respectively (Table 1).
Pre-analysis | Chromatography | Mass spectrometry | Analysis | Post-analysis | ||
---|---|---|---|---|---|---|
Source | Methods | chromatographic technique = Instrumentation (method) | Mass analyzer = Instrumentation (method) | Database search | Number of lipoproteins and apoproteins | References |
Mice C57BL/6 J: plasma | Gel filtration/size exclusion chromatography, phospholipid-containing particles using CSH | LC-ESI = C18 reverse phase column (GRACE; 150 × 0.500 mm) | Quadrupole/TOF = 4800 scans, mass range: 300–1800 | Swiss-Prot protein knowledge base for | VLDL/LDL: 32, HDL: 104; lipid poor lipoproteins: 55 | [37] |
Human serum (HDL) | Trypsin, delipidation, gold nanoparticles and LDI-MS, EDX, SPE, FT-IR | MALDI-MS = nr | Swiss-Prot, Lipidmaps database | HDL (delipidation): 10 (prior), 6 (after). HDL (anion exchangers): 23 | [38] | |
Mice C57BL/6 J: apoA-I KO and apoA-II KO, apoA-I KO and WT: plasma | Particles of CSH. Plasma separation by size exclusion chromatography | LC-ESI = column (C18 reverse phase (150 × 0.500 mm)) | Quadrupole/TOF = MS/MS. tolerance were set to ±35 PPM, and up to 3 missed tryptic cleavage sites were allowed | UniProtKB/Swiss-Prot Protein Knowledgebase, Peptide Prophet algorithm | HDL: WT:25 vs. ApoA-I KO: 21; ApoA-II KO: 11 vs. WT: 14; and ApoA-IV: 6 KO vs. WT: 7 | [28] |
17 Subjects (exposure to organic pollutants): plasma (HDL) | Ultracentrifugation (290,000 g, 15°C, 4 h) | nLC: Column C18, ESI | Linear ion trap-Orbitrap: CID. ms/ms: 0.6 Da | MaxQuant v1.5.0, human Uniprot/Swiss-prot database | LCMS permit identified the pathway of interaction between HDL-proteins | [39] |
34 Men (2 dietary: weight loss/high car): plasma (LDL, ApoC-III) | ELISA assay | MALDI-TOF: 500 laser shots mass spectra | Detection and concentrations values of 12 apoC-III glycoforms | [40] | ||
Mice (female, LDLr−/−, 8 week old): plasma (VLDL, LDL, HDL) | FPLC, ELISA assay | LC | Orbitrap: mass tolerance: 0.8 Da | Swiss-Prot database | HDL: 91 LDL: 49 VLDL: 39 | [41] |
1000 Children (6–8 years, Nepal rural zone): plasma | Cation exchange chromatography | LC | Orbitrap | Refseq 40 database | Apo-AI, Apo-AII, Apo-CIII | [42] |
458 Children (6–11 years, exposure to environmental tobacco smoke): serum (HDL, LDL) | SPE-HPLC-TIS-MS/MS | LC detected various polyfluoroalkyl substances in serum A negative association PFOS and non-HDL | [43] | |||
57 Males (exposed to arsenicum): plasma, urinary (LDL, HDL, Lp(a), Apo-A1, Apo-B) | Centrifuged at 3500 rpm for 10 min | ICP-MS: extraction voltage −100 V, Rf power 1400 W, focus voltage 12 V, and nebulizer gas flow rate (using a Burgener Miramist nebulizer) 0.83 L/min. Dwell times were 50 ms for 75As and 10 ms for internal standard (72 Ge) | ICP-MS: Potential risk of the arsenic on lipoproteins and apolipoproteins | [44] | ||
Human healthy, normolipidemic males: plasma (LDL) | Gel filtration chromatography, ultracentrifugation. | nLC: column (IntePepMap 100, C18, particle size 3 um), flow rate = 300 nL/min ESI: 2.5 kV, 150°C | Triple quadrupole TOF: mass tolerance: 50 mDa, 350–1800 | UniProtKB/Swiss-Prot Protein Knowledgebase, Peptide Prophet algorithm | LC-MS permit the abundance protein as well as antioxidant activity | [30] |
110 Samples (purchased) | Phospholipids: UHPLC: column (2.1 × 100 mm, 1.7 uM particle), flow rate: 0.7 mL/min, ESI | Qtrap: MRM scanning, negative and positive mode | Multiquant software functions, JMP (SAS Institute) | LC-MS analysis of serum/lipoproteins | [45] | |
23 Healthy volunteers: serum | Gel filtration chromatography, sequential ultracentrifugation, immunoassay | UPLC: column (UPLCR BEH C8), floe rate: 450 uL/min., 60°C, autosampler: 4°C ESI | Triple-quadrupole: positive ion mode. Quantification of plasmalogens: Capillary voltage: 3500 V, source temperature 80°C, desolvation: 400°C, cone voltage: 35 V. CE: 20–32 eV | LCMS: distribution of each molecular species in plasmalogen and choline plasmalogen | [46] | |
Healthy volunteers: plasma (VLDL, LDL, HDL) | Ultracentrifugation, SDS-PAGE, size exclusion chromatography, circular dichroism, spectroscopy, spectropolarimeter | MALDI-TOF: 337 nm nitrogen laser, positive ion: 20 kV | Identification of apolipoproteins released from VLDL by mass spectrometry | [47] | ||
20 Patients with lipoproteins (a) (18–70 years): plasma (LDL, HDL) | Ultracentrifugation, ELISA | LC: solid-phase extraction | Triple Quadrupole-linear ion tramp: SRM. (energy collision: 34 V, Q1: 786–788; Q2: 1069–1072 | LC-MS system: concentrations of lipid, lipoprotein and apolipoprotein | [48] | |
Healthy donors (nonlipidemic, 24–65 years, purchased samples): plasma (HDL, LDL) | Ultracentrifugation (330,000 g, 6 h), SDS-PAGE and Western blotting, Negative stain electron microscopy | UPLC: column (Kinetex EVOC18), ESI | Triple-quadrupole: lipid species were analyzed by selected reaction monitoring (from 141 to 369 | Extraction and ionization efficacy by calculating analyte/ISTD ratios (AU) and expressed as AU/mg protein | LCS permit the separation of a mixture in HDL protein | [49] |
12 Healthy male (36–67 years): plasma (HDL, LDL) | Ultracentrifugation (40,000 rpm, 44 h, 15°C), fractioned, apoA-I was detected by Western blotting, internal standars | HILIC-UHPLC-FLD: Glycan chromatography column, 150 × 2.1 mm i.d., 1.7 μm BEH particles, flow rate of 0.56 mL/min | MALDI-TOF-MS: 25 kV, acceleration voltage: 140 ns extraction delay, mass window: 1000–5000 | LDL: 18 HDL: 22 N-glycome of human plasma lipoproteins | [50] | |
16 Healthy adults: plasma (HDL, apoA-I, apoB) | Ultracentrifugation. PRM analysis (shotgun proteomics experiments). | UPLC: flow rate: 0.6 uL, column (Xbridge BEH C18) ESI | Orbitrap: PRM mode, isolation window: 2 Th, HCD: 27%, orbitrap analyzer: 15,000 resolution, AGC: 5 × 104, maximum ion time 30 ms | PeptideAtlas mass spectral database | Meal macronutrient content HDL composition in the postprandial state | [51] |
47 Volunteers: serum (HDL, non-HDL). | Anti-apoAI magnetic nanoparticles (10 mg) and serum (5 μl) were mixed FTIR, X-ray diffraction | ID/LC/MS system: LC: column (waters symmetry C18), flow rate: 0.3 mL/min. APCI: corona current: 5 uA, source temperature: 450°C | API 4000 tandem mass spectrometer (triple-quadrupole): Collision energy: 26 eV, collision exit potential: 6 V | ID/LC-MS permit the monitoring serum in clinical settings to dyslipidemia and atherosclerosis | [52] |
Chromatographic and spectrometric methodologies used recently in lipoproteomic analysis.
SRM: selected reaction monitoring. HPLC: high performance liquid chromatography. HILIC-UHPLC-FLD: hydrophilic-interaction ultra-high-performance liquid chromatography with fluorescence detection. LC: liquid Chromatography. UPLC: ultra-performance liquid chromatography. MALDI: matrix-assisted laser desorption/ionization. TOF: time of flight. Qtrap or LTQ linear trap: triple quadrupole-linear ion trap. CE: collision energy. MS/MS: tandem mass spectrometry. PRM: parallel reaction monitoring. AGC: automatic gain control. ID/LC/MS: isotope dilution liquid chromatography mass spectrometry. ESI: electrospray. nESI: nano-electrospray. FPLC: fast protein liquid chromatography. MRM: multiple reaction monitoring. HILIC: silica-based and solid-core reverse phase after hydrophilic interaction. ICP-MS: Inductively coupled plasma-mass spectrometer. SPE: solid phase extraction. SPE-HPLC-TIS-MS/MS: solid phase extraction coupled to high performance liquid chromatography-turbo ion spray ionization-tandem mass spectrometry. SEC-FPLC: size exclusion chromatography by fast protein liquid chromatography. APCI: atmospheric pressure chemical ionization. CID: collision-induced dissociation. LDI-MS: laser desorption/ionization mass spectrometry, EDX: dispersive X-Ray Spectroscopy. CSH: calcium silica hydrate. WT: wild-type. KO: knockout. FTIR: Fourier transformation infrared.
There are several challenges in the lipoproteomic analyses performed with the most advanced mass spectrometry methods. Some of them are the abundance of proteins from the biological source and the lipoprotein(s) purification steps, which conditioned the protein content and constitution. However, if this obstacle can be overcome, the mass spectrometry analysis has been demonstrated to be a useful tool to identify a diverse array of proteins related to lipoproteins, avoiding aberrant integration of unexpected proteins by reducing the suppression of ionization at high peptide resolution [53].
On the other hand, the nuclear magnetic resonance (NMR) technique permit, besides protein identification, can provide information of the lipoproteins at both molecule and atomic levels under physiological or ‘near-physiological’ conditions. The signal most used to quantify lipoproteins is the methyl signal because give a specific response in the lipids that travel inside the lipoproteins [54, 55]. In this way, proton spectroscopy has been the most used nuclear magnetic resonance technique to quantify lipoproteins, but it is not the only one as will be seen later (Table 2).
Pre-analysis | | Spectroscopy experiment NMR type | Post-analysis | References | |
---|---|---|---|---|
Source | isolation method | |||
133 Caucasian participants (T2DM, >18 years): serum (VLDL, LDL, and HDL) | 1H: FT, 400 MHz | Determinate the lipoprotein subtraction characteristics | [56] | |
98 people (T2DM/ nor T2DM): plasma (GlycA vs. Lp-PLA2) | Centrifugation (1400 g, 15 min) | 1H: 400 MHz, 47°C, CaEDTA resonance at 2.519 ppm was used as the internal chemical | GlycA is correlated with LP-PLA2 in plasma (person without T2DM/MetS) | [57] |
23 patients with primary aldosteronism: plasma (HDL, VLDL, LDL, ApoB, and ApoA-I) | Immunoturbidimetric assay | 1H: 400 MHz, 47°C. (LipoProfile-3 algorithm) | Circulating LDL may contribute to adrenal steroidogenesis in humans | [58] |
115 nondiabetic women (35–55 years, mediterranean diet, physical exercise, 2 years): plasma (HDL. LDL) | 1H | Lipoprotein size, particle and subclass concentrations | [54] | |
Human serum (purchased) spiked into phlebotomy tubes (LDL, HDL) | Centrifugation (3000 g, 5 min, 4 h) | 1H: 600 MHz | Lipoprotein subclass analysis standardized by tube type and tube size to prevent risk of analytical interference. | [59] |
Patients with HFrEF (782), HFpEF (1004), and no HF (4742): plasma (HDL) | NMR LipoProfile-3 algorithm | Quantify concentrations of HDL. Phenotyped cohorts of HFrEF, HFpEF, and patients without HF | [60] | |
4897 subjects: plasma (LDL) | 1H: 400 MHz | Differentiate in the size of particle in LDL profile | [61] | |
309 patients (MACE): plasma (HDL, LDL, and VLDL). Control:902 | 1H | Neither baseline HDL nor the change in HDL on treatment with dalcetrapib or placebo was associated with risk of MACE after ACS | [62] | |
Normal volunteer: plasma (HDL, LDL, andVLDL) | Sequential ultracentrifuge | 1H, 13C, 15N: 600.55 MHz, 47°C, different pressures | Show the spatial arrangement, phase behavior and molecular dynamics in the particle core | [55] |
3446 participants (HDL, LDL, and VLDL) | 1H: LipoProfile-3 algorithm | Association between FGF21 and NAFLD | [63] |
NMR methodology applied recently in lipoprotein-based analyses.
MHz: megahertz (106). MetS or MS: metabolic syndrome. T2DM: type 2 Diabetes mellitus. FT: Fourier transforms. CaEDTA: EDTA mono calcium. GlycA: glycoprotein acetylation. Lp-PLA2: lipoprotein-associated phospholipase A2. HFrEF: reduced ejection fractions. HFpEF: preserved ejection fraction. HF: heart failure. MACE: major adverse cardiovascular events. FGF21: fibroplast growth factor21. NAFLD: nonalcoholic fatty liver disease.
Furthermore, not only 1H NMR has been the technique used for the study of lipoproteomics but also the two-dimensional NMR techniques have been used. The two-dimensional heteronuclear 13C▬1H chemical-shift made it possible to analyze macromolecular complexes like HDL, but with a limited resolution in reduced peaks above 50 ppm and the limited resolution in the 29–33 ppm region, inclusive with artifacts that would later be discarded by the spectra of one dimension (1H and 13C) [64].
In addition, the material to be used for the spectroscopic analysis should be the optimal one to avoid contamination, as in the case of a tube used for the collection of biological material and used in clinical research, which should be specifically for the analysis of lipoproteins [59].
Also, the experimental conditions do not always favor the use of NMR for the analysis of lipoproteins. Thus, mass spectrometry permits the particle identification via LC-MS system in contrast to NMR spectroscopy, which failed. Due to that, the NMR spectroscopy makes HDL particle quantification only in a physiological setting: full serum or plasma but not in HDL-containing suspensions [49]. Therefore, the technician must consider the biological and technical variables for an assertive lipoproteomic analysis.
The lipoproteomic analyses have been focused on understanding the functional mechanisms underlying apolipoproteins and lipoprotein-associated proteins that can be used to develop new diagnostic and/or prognostic biomarkers for many lipoproteins’ metabolism-related illness.
The HDL lipoprotein fraction has been the most studied according to lipoproteomic relationships with different diseases. Among the HDL-associated proteins, ApoC-III levels have been seen increased in the patients with either a lupus nephritis (lupus erythematosus) or with a cerebral lacunar infarction, which could be related with a reduced anti-inflammatory activity of HDL particles [65, 66].
However, HDL-carried ApoC-III has been more implicated in cardiovascular disease (CVD) risk. One of the first evaluations was performed with coronary artery disease patients, which exhibit increased levels of ApoC-III [67]. Recently, in a cohort study, it was demonstrated that HDL-carried ApoC-III is related to a higher risk for coronary heart disease [68].
In addition to ApoC-III and ApoC-II, other HDL-associated apolipoproteins, were proposed as biomarkers for CVD risk in patients with chronic hemodialysis [69]. The ApoC-III/ApoC-II/ApoE levels in VLDL lipoproteins, independent of HDL, were associated with incident CVD, which supports the concept of targeting triacylglycerol-rich lipoproteins to reduce the CVD risk [70].
Other HDL-associated apolipoproteins have been associated with cardiac pathologies. For example, ApoA-I, ApoA-IV, ApoE, and ApoL1 levels have been seen enriched in HDL3 fraction from patients with acute coronary syndrome (ACV), with a concomitant reduction of these apolipoproteins in the HDL2 fraction [71]. Furthermore, ApoC-I was significantly decreased in the HDL particles of coronary heart disease (CAD) patients in comparison to normal individuals [72]. The existence of CAD has recently been correlated with an HDL apolipoproteomic score, independent of circulating ApoA-I and ApoB rates and other typical cardiovascular risk factors [73].
Regarding HDL-associated proteins, many of them have been related to heart illness. For example, serotransferrin, haptoglobin, hemopexin, complement factor B, ras-related protein Rab-7b, and paraoxonase-3 (PON3) levels have been seen reduced in HDL from patients with some cardiac pathology. Meanwhile, PON1, alpha-1B-glycoprotein, vitamin D-binding protein, alpha-1-antitrypsin (A1AT), acid ceramidase, serum amyloid A and P proteins, sphingosine-1-phosphate, filamin A, and pulmonary surfactant-associated protein B are increased in HDL fractions from patients with cardiometabolic disorders [69, 71, 72, 74, 75].
Diabetes is, perhaps, the major controllable risk factor for CVD. In particular, related to the HDL fraction, ApoA-I, ApoA-II, ApoA-IV, ApoE, ApoJ, as well as PON1, transthyretin, complement C3, and vitamin D-binding protein have shown changes in patients with type 2 diabetes (T2D) [76, 77]. In contrast, individuals with type 1 diabetes (T1D) had proteomic alterations of their HDL particles. For example, the complement factor H-related protein 2 was elevated, independent of glucose control, in T1D patients in comparison to healthy controls. Also, the optimal glucose control corrected the elevated levels of the alpha-1-beta glycoprotein and inter alpha trypsin inhibitor 4. Furthermore, the HDL particles in T1DM individuals, independent of glucose control, exhibit a higher abundance of irreversible post-translational modifications of HDL-associated apolipoproteins [78, 79].
Also, in psoriatic patients, the levels of HDL-associated ApoA-I exhibit a significant reduction, whereas levels of apoA-II, serum amyloid A, C3, and A1AT, among other proteins were increased [80]. Besides C3 and C9, complement factor B, as well as ApoJ, fibrinogen, haptoglobin, and serum amyloid A have been also increased in HDL fraction from patients with rheumatoid arthritis [81]. Taken together, these results suggest that HDL-associated proteins could be involved in anti-inflammatory properties in chronic illness.
Concerning other diseases, the proteome of HDL particles has been used to identify protein markers. In nonalcoholic fatty liver disease, including individuals with nonalcoholic steatohepatitis, changes in the abundance of HDL-associated proteins such as antithrombin III and plasminogen have been identified [82]. Although not directly to HDL particles, proteomic analysis of some HDL-related apolipoproteins has been associated with viral diseases. For example, a change in the expression level of ApoA-I was suggested as a specific and appropriate alternative to conventional HIV diagnosis and progression measurements in clinical research settings [83]. Increased concentration of ApoM in sera patients with HBV infection have been detected [84]. Recently, a downregulation of ApoA-I and ApoM levels have been associated with the severity of COVID-19 infection [85].
In addition to HDL, only other few lipoproteomic studies have been developed to discover changes in lipoproteins-associated proteins in diverse pathologies. Proteomic studies of LDL have reported that carry apolipoproteins AI, A-II, CI, C-II, C-III, C-IV, D, E, and F, in addition to apoB, as well as clusterin, C3, C4a, and C4b, and PON1 that are also associated to this particles [86]. Serum amyloid A levels were found to increase in all lipoprotein fractions, especially in LDL from atherosclerotic patients [24]. An enriched content of all the apoC-III isoforms and a lower content of apoA-I, apoC-I, and apoE were detected in the sdLDL from subjects with metabolic syndrome and subclinical peripheral atherosclerosis [87]. VLDL, IDL, and LDL fractions from Alzheimer patients exhibit low levels of complement C3 [88].
Target discovery, which is an important step in the drug development process, includes discovering and validating targets associated with the diseases. It is increasingly recognized that, in many instances, metabolomics is used to identify novel biomarkers, which can help in the discovery of therapeutic strategies for many diseases [89].
Until now, lipoproteomics has proved to be an effective method for identifying candidate cardiovascular disease markers. Studying the profiles of protein expression in drug-treated patients contributes to the discovery of multiple drug-specific targets. Traditionally, statin therapy has proven efficacy in reducing cardiovascular events, but as aforementioned, the identification of HDL-associated proteins is variable. The statin therapy has a notorious effect on the increment of A1AT associated to the large fraction of HDL particles enhancing its anti-inflammatory functionality, which may interfere with the statins outcome on reducing cholesterol levels [37]. In addition, a relationship between the CAD treatment and the HDL proteome was demonstrated using statin and niacin therapy, observing that ApoE and ApoC-II levels are enriched in the HDL3 fraction, which contains less ApoJ levels [90].
The increment in the HDL levels by CETP inhibitors is another biomarker that has continued to be disappointing in clinical research. In fact, in patients with deficiency of CETP (CETP-D), the HDL particles are enriched with ApoE, angiopoietin-like3 protein, and complement regulatory proteins such as C3, C4a, C4b, and C9 that could be associated to the increased atherogenic profile in CETP-D patients [91].
Thus, it is important to consider that among the diverse HDL populations not all possess a cardioprotective effect [92]. Therefore, we need a better knowledge of the protein cargo of the HDL populations with anti-atherogenic actions. A comprehensive understanding of the HDL proteomics can lead to the design of more effective anti-atherogenic drugs based on the activity of HDL-associated proteins that will provide new therapeutic strategies at the molecular level.
In the dynamic drug discovery process, the different proteomic methods, which include MS-proteomics, expand beyond the general aim of target drug discovery. It must consider the drug-protein interactions as well as elucidate the mode of action of candidate drug molecules [93]. Thus, novel HDL-based therapeutic agents, besides the traditional statins, will need consider to the functional HDL lipoproteomics to characterize the interaction of the drug with the HDL-associated proteins. This is necessary as an attempt either to elucidate the mechanism of action by direct drug-protein interaction or as a biomarker for drug validation by monitoring the pharmacological effect through an increase of HDL populations with an anti-atherogenic protein cargo.
The lipoproteins can be involved in different pathologies related to lipid metabolism such as atherosclerosis, cardiovascular risk, obesity, metabolic síndrome, and diabetes, among others. However, the protein cargo of these particles has been associated with several functions, which differ from the amply recognized as structural composition and receptors recognition during their function as lipid transporters. For this reason, the identification of variants of apolipoproteins and lipoprotein-associated proteins has been an important referent to detect new biomarkers. In fact, as we described here, several methodologies have been developed to improve the lipoproteomic profile.
Despite these studies, the majority concord that both biological source and lipoprotein purification are important steps to avoid protein contamination, principally from samples that are used directly as serum or plasma. Also, the most used method to identify the lipoprotein-associated proteins is the mass spectrometry, but some limits are presented that depend on the platforms to apply this methodology. Also, it is important to highlight that not everyone has the facility to use this methodology and it is necessary to develop new methodologies to apply in clinical fields to ensure discoveries about these proteins both in new lipoproteins’ fractions and new diseases.
Also, the lipoproteomic analyses could be a new clinical area to evaluate the therapy of the pathologies described here as prognostic analytes. In this sense, the HDL lipoproteomics is, perhaps, the more advanced field considering the evaluations of populations with statins’ treatment. However, novel HDL therapeutic agents must consider the functional lipoproteomics of these particles. Finally, these lipoproteomes can help us to describe the molecular mechanism to understand the interaction of apolipoproteins as well as the lipoprotein-carried proteins to support the other omics such as lipidomics and metabolomics.
This work was supported by the Autonomous University of Mexico City (UACM) awarded to MEAS.
The authors declare no conflict of interest.
The European Society of Human Reproduction and Embryology (ESHRE) report from February 2018 shows that Europe continues to lead the world with approximately 50% of all reported in vitro fertilization (IVF) cycles. The latest figures are available for 2014, and these show that 39 European countries reported almost 800.000 cycles, which compare to 150.000 cycles in the USA or 65.000 cycles in Australia and New Zealand (https://www.eshre.eu/~/media/sitecore-files/Guidelines/ART-fact-sheet_vFebr18_VG.pdf?la=en). Although there are no official reports from China, it is estimated that this country performs more than 800.000 IVF cycles with a tendency to grow. In summary, the ESHRE estimates that around 2.5 million IVF cycles is performed every year and that about 500.000 babies are born following the IVF.
\nThe pregnancy rate depends on the embryo quality, and in Europe in 2014, the mean pregnancy rate per embryo transfer was 35% after IVF, 33% after ICSI, 30% after frozen embryo transfer, and 59% after egg donation. It is observed that pregnancy rates are higher in younger patients (<35 years).
\nThere are large differences between countries concerning the number of embryos transferred and the resulting multiple births that can occur following the multiple embryo transfer. Multiple pregnancies are classified as high-risk pregnancies and pose a significant health risk to both the mother and baby. However, regardless of that, the practice of multiple embryo transfer is still present, and it is being widely used in order to increase the chances of pregnancy. Currently, efforts are made to reduce the number of multiple embryo transfer, and a mean of 1.81 embryos per transfer is being reported. In Europe, according to the report of the European Society of Human Reproduction and Embryology, the number of multiple delivery rates for embryo transfers dropped from 26.9% in year 2000 to 17.5% in 2014 (latest data available), and it is expected to decline further. Some countries, e.g., Sweden, has reached a very low multiple delivery rate, and the single embryo transfer was performed in more than two-thirds of all transfers.
\nThe importance of IVF in society is also highlighted by the 2010 Nobel Prize which has been awarded to Robert Edwards who is, together with Robert Steptoe, the “father” of the fist “IVF-Baby.”
\nThe IVF process is an utmost stressful, both physically and psychologically, for both parents but primarily for the mother-in-being. A number of physical examinations and medical procedures are being performed in order to ensure that the process will lead to a successful pregnancy.
\nDuring the IVF process, especially when decisions are made which embryo shall be transferred in order to ensure the greatest chance for success, the best possible decision, based on the fast and accurate information on the status of the embryo, is needed. Due to the increasing age of mothers-to-be, during the past years, a steady increase in pregnancies following artificial reproductive techniques (ART), namely, IVF and intra-cytoplasmatic sperm injection (ICSI), was observed. The reasons for this development must be seen in economic, educational, and social factors, which lead to steadily rising rates of elderly patients. With increasing age, fecundability and fertility decrease. However, the advances made in reproductive medicine itself might be one of the reasons, why an increasing number of women delay childbearing, hoping for late babies and consciously making decisions to use the help of reproductive medicine.
\nThe elevation of pregnancy rates for IVF procedures is certainly needed in order to reduce the burden on patients and lower costs affiliated with the procedures, which very often must be repeated until a pregnancy is reached and the embryo develops to successful birth. New technologies and methods more efficient than the current approach using PGS are needed. One new approach could be the analysis of the embryos’ proteome during the IVF process. This means to analyze the specific proteins that are secreted by the embryo in the IVF surrounding of the culture medium. Routinely, the medium left after embryo transfer is being discarded. The multiple embryo transfer occurring during the IVF procedure is necessary after the fertilization step, and the spent media are a rich source of biological material that can be used for diagnostic purpose. This procedure of embryo transfer to a new culture medium is essential for the embryo’s development and occurs daily or at least when the embryos have achieved the stage of embryoblast at day 4.
\nThe spent medium can be useful for a detailed examination of proteins and lipids that were secreted by the embryo during each stage of development and can be used for the estimation of the “embryo quality” and selection of corresponding embryos for primary transfer.
\nAn overview of technologies and approaches being used and a simplified experimental approach are presented in this chapter. The method used in our laboratory is used just as an example of the technologies used, and a number of different approaches will be discussed.
\nEmbryo cultivation medium is usually being discarded during the IVF procedure although it can be used for multiple diagnostic and prognostic procedures. Certainly, ethical and moral guidelines and procedures must be observed, and the ethical board or other corresponding bodies must approve of the use of this material. The following experiments have been performed by using cultivation media from IVF media upon approval from the ethical boards of the Medical University in Vienna and the University of Linz in Austria. Samples were collected during different stages of embryo development and analyzed using methods established for analysis of low samples amounts.
\nTrypsin for protein digestion was purchased from Promega Inc. (Vienna, Austria). Solvents for HPLC—methanol (MeOH), acetonitrile (AcN), 2,2,2-trifluoroethanol (TFE), formic acid (FA), heptafluorobutyric acid (HFBA), iodoacetamide (IAA), triethylammonium bicarbonate (TEAB), and dithiothreitol—were purchased from Sigma-Aldrich (Vienna, Austria).
\nOne of the most important steps when analyzing embryo cultivation media is the depletion of serum albumin present in these samples. Tarasova et al. [1] described the innovative method of using immobilized antihuman albumin antibodies for depletion of small sample volumes. Briefly, for depletion of culture media samples from selected embryos, the sample was diluted using the phosphate buffered saline buffer (pH 7.4) consisting of 0.01 M phosphate buffer, 0.0027 M KCl, and 0.14 M NaCl. This buffer was further also used as a washing buffer A upon the sample loading. In order to ensure full sample recovery from the depletion column, a ready-to-use elution buffer from Agilent (pH 2.25) (Agilent Technologies, CA, USA) was used as buffer B. We have developed a new column for the depletion of human albumin by immobilizing the antihuman albumin antibodies to the monolithic support disk, the CIMac-HSA column, especially for analysis of small sample amounts, which also occur in IVF samples. This column was used in an ICS-5000 inert HPLC system (Dionex-Thermo Scientific, Germering, Germany) for albumin depletion with a column flow rate of 0.3 mL/min. Upon sample injection, the loading and washing buffer A was pumped through the column for 5 min, and the flow-through fraction was collected (V = 350 μL). This fractions’ volume corresponds to the full absorbance peak and contains all proteins that were not trapped on the column. The albumin was trapped by the interaction with the antibodies on the column’s surface, and it was eluted by increasing the amount of the eluting mobile phase from 5 to 10 min. The column was, finally, flushed with the loading buffer A for additional 4 min, and this step was followed by an additional washing step with buffer B and, finally, equilibrating step using, again, the loading buffer A for 13 min. The total time for completing this depletion protocol is 30 min when applying the column flow rate of 0.3 mL/min. During this time, the very important column wash step and the complete re-equilibration of the column preceding the next depletion run is being perfromed. The flow rate used was selected for maximizing the protein’s interaction time and was a compromise between the speed and efficacy of operation. If desired, higher column flow rates can be used without losing much of the column’s performance [2], but this shall be carefully examined and optimized.
\nProteins in both collected fractions and non-depleted samples were depleted using a standard protocol with trypsin. Due to a high concentration of phosphate buffer in collected fractions, 1 M triethylammonium bicarbonate (TEAB) was added to the fractions in order to reach a final concentration of 50 mM TEAB and to dilute the phosphate buffer. For protein denaturation, 10 μL of 0.1% (w/v) Rapigest in 50 m MTEAB were added to all fractions, and reduction of disulfide bonds was performed using dithiothreitol (final concentration of 5 mM DTT) and incubating the reaction mixture for 30 min at 60°C.
\nFor a successful trypsinization, alkylation was performed using iodoacetamide at a final concentration of 15 mM IAA. Upon addition of IAA, the sample was incubated at room temperature, in the dark, for 30 min. An excess of iodoacetamide is inhibiting the trypsin action and needs to be neutralized, which was achieved by adding 2 μL of 50 mM DTT in 50 mM TEAB, vigorous mixing for 2 min at room temperature. Finally, 10 μL of 0.2 μg/μL trypsin solution were added, and samples were incubated for 16 h at 37°C in an incubation oven. Tryptic activity was stopped by acidifying the solution with 1% TFA solution.
\nFor LC–MS/MS analysis, 20 μL of the digested and diluted sample (aqueous 0.1% TFA at a ratio of 2:3 (v/v)) were injected for LC–MS/MS analysis.
\nA Dionex UltiMate 3000 RSLC nanoLC system (Dionex-Thermo Scientific) was used for the nanoHPLC separation of tryptic peptides. Mobile phases used for chromatographic separation of tryptic peptides were as follows: (A) 5% of acetonitrile in aqueous 0.1% formic acid and (B) methanol, trifluoroethanol, water and acetonitrile (30:10:10:50 (v/v/v/v) and 0.1% of formic acid). The sample was loaded onto the trap column for washing the residula salts and focusing of the analytes as a small sample front by using loading mobile phase consisting of 0.1% aqueous TFA chilled to 3°C.
\nThe nonlinear gradient of 75 min total running time was used for HPLC separations of tryptic peptides. The gradient was composed using sequential linear steps:
1.0% B min−1 for 7 min.
0.5% B min−1 gradient for 38 min.
1.5% B min − 1 for 20 min.
3.0% B min−1 for 10 min.
The HPLC flow was introduced into the maXis Impact UHR TOF mass spectrometer (Bruker Daltonics, Bremen, Germany), and the LC–MS/MS analysis was performed in triplicate for each sample. For peptide fragmentation (MS/MS), collision-induced dissociation was used, and all MS/MS data were acquired in a 300–2000 m/z range using a three-second cycle without further optimization of the instrument’s parameters. In order to prevent unnecessary fragmentation of ions with low intensity, the lower threshold for precursor ions was set to 1000 counts. Due to tailing of some peptides and frequently observed co-elution of peaks, a dynamic exclusion was employed, and the duration was set to 120 s. In case where the precursor ion showed higher intensity after the exclusion period of 120 s, it was reconsidered for an additional MS/MS event.
\nDatabase search for protein identification was performed using the all-taxonomy Swiss-Prot database (http://www.uniprot.org). For this search, X! Tandem [3] with following parameters was used:
Precursor mass tolerance of ±10 ppm.
Fragment mass tolerance of ±0.3 Da.
Maximum of two missed cleavages were allowed.
Some posttranslational modifications of peptides due to the sample preparation and due to biological processes can be expected. For the experiment described, cysteine carbamidomethylation was selected as the fixed modification and methionine oxidation; phosphorylation of serine, threonine, and tyrosine residues, as well as the acetylation of lysine and N-terminal residues, were selected as variable modifications.
\nAll data analysis for the experiments described in this manuscript was analyzed using the method described by Tarasova et al. [1]. The use of pyteomics.pepxmltk converter (https://pypi.python.org/pypi/pyteomics.pepxmltk) and search was described elsewhere [4]. Briefly, this platform was used to convert X! Tandem files to the standard pep.xml format and perform data analysis. All identifications, upon database search, were filtered to meet the requirement of the 1.0% FDR at peptide level. Search results were submitted for a post-search validation using the MPscore software, described earlier [5]. Quantitative information on identified proteins is a substantial requirement for determining the differences between biological samples, and all proteins fulfilling the identification and validation requirements were quantified using the label-free quantitation approach called normalized spectral index (SIn) (Griffin et al.) [6].
\nThe presence of albumin in cultivating medium is necessary for the normal development of embryos. However, its presence is a significant burden for proteomics analysis, and it must be removed prior to further analysis steps. The removal of albumin has been extensively discussed and described in a number of publications [1, 2, 7, 8, 9]. Different groups have used a number of methods such as immunodepleting chromatography, molecular weight cutoff filters, peptide libraries, size exclusion chromatography, etc. All these methods have some advantages but also show disadvantages. In case of depleting the albumin from IVF cultivating medium, the very low sample volume (max. of 40 μl) must be taken into consideration. Furthermore, depletion method must be performed fast and must be reproducible over a large number of samples, if intended to be used for fast analysis of clinical samples that shall help making the decision on which fertilized oocyte shall be transferred first and which ones shall be frozen for later procedures. The use of a novel immunoaffinity-based convective interaction media analytical columns (CIMac) for depletion of HSA (CIMac-HSA) was performed in this study, and it proved that it can be used for fast and reproducible albumin depletion from minute sample amounts. The column’s architecture and the convective flow-through columns’ channels enable a flow rate-independent binding capacity and excellent chromatographic resolution. These characteristics give CIMac-αHSA column some important analytical benefits like shorten time of analysis in comparison to common chromatographic depletion of albumin using silica-based columns, which is an extraordinary important parameter for clinical use. The albumin content in different batches of cultivating medium differs strongly, and it also differs strongly between different suppliers. Therefore, the depletion method must be selected in a way that can be applied for a variety of samples. However, independently of the selected methods, the removal of albumin from the cultivating medium results with a number of identified proteins of which some can be of importance for embryo development and for later pregnancy development.
\n\nFigure 1 shows two SDS-gel lanes for the separation of two media upon embryo transfer. Since albumin originates from different donors and is being mixed at the manufacturing site, it will certainly contain a number of other proteins that may interfere with proteins secreted from the developing embryo. Also, secreted proteins might bind on albumin and, therefore, be “invisible” for proteomics analysis.
\nLanes 2 and 4 show an example of the separation of proteins secreted in culturing media on silver-stained SDS gel. As expected, large spots for human serum albumin (HSA) are observed for both samples with significantly lower amounts of other proteins. Since the culturing media have been declared to contain only HSA, the proteins below are secreted from the fertilized oocyte (lane 2) and unfertilized oocyte (lane 4).
In addition to that, the human proteome, as any proteome, is dynamic, and it is constantly changing through internal and external stimuli. Proteins being translated from RNA are directly responsible for cellular function, but different gene expression studies have, unfortunately, shown that a reliable prediction of proteins’ function or abundance cannot be made. As for the human proteome, significant advances have been made for the analysis of the whole proteome and of the proteome of different disease states [3, 5, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20]. Bearing all of it in mind, the analysis of proteins obtained from the cultivating media is challenging, and the results are not always conclusive.
\n\nFigure 2 shows the results obtained upon analyzing four different cultivating media used in routine IVF procedures and upon embryo transfer.
\nIdentified proteins and their relative quantities in albumin-free fractions after performing albumin depletion of culturing media samples on two different depletion columns. Proteins identified either in the CIMac-αHSA FT or the Seppro FT1 fractions are labeled with B and S, followed by Swiss-Prot protein ID. Median values of normalized spectral indexes (SI) and standard deviations are shown. Median SIHSA in non-depleted samples is marked with red dashes. More than two peptide-spectrum matches were assumed for each of the listed proteins according to Tarasova et al. [
Dyrlund et al. [21] have performed the analysis of unconditioned commercial embryo culture media and have identified a number of proteins upon depletion and digestion of a total of 5 mL of media. The amount of 5 mL, however, will never be available if samples are retrieved during the actual IVF process. However, this analysis showed that this amount contains an astounding amount of 25 mg of albumin. Here, a total of 111 proteins with different concentrations in different batches of the media were identified in addition to albumin. The sample of unconditioned media also contained eight proteins previously suggested as possible markers of embryonic viability, e.g., proteoglycan-4, serotransferrin, vitamin-D-binding protein, ubiquitin, etc.
\nAnother study performed on IVF medium examined the batch-to-batch variations and showed that variation in both protein amounts and protein identifications can be expected [22]. When studying these media, it is important to pay attention to the fact that both media, the control and the media where embryos grow, must be from the same batch in order to make valid comparison.
\nSpent IVF medium is a valuable source for proteins that can be used as putative biomarkers in IVF. The major focus of researchers has been on secreted proteins, but the proteins already present in the medium can be equally valuable and must be considered in the pursuit for biomarkers of embryos’ “quality.” It is very possible that proteins already present in the medium may be necessary for embryo development, and the uptake or degradation of specific proteins might correlate to ascertain embryo development or lack thereof. Therefore, these media must be evaluated for their potential to differentiate embryos’ success rates and track the proteins, which can be potential biomarkers. Additionally, the safety of these proteins in culture media for the offspring should also be evaluated.
\nProteins identified by Dyrlund et al. were also reported by Katze-Jaff upon analysis of the secretome of the individually cultured human embryos, and a hint was made that these proteins could be related to embryo’s morphology and thus its viability [23, 24, 25]. They have reported that ubiquitin was upregulated in developing blastocytes as compared to degenerating blastocytes. However, there was no correlation between the ubiquitin upregulation and the observed pregnancy rates.
\nCortezzi et al. [26] reported the identification of proteins in both positive and negative groups of embryos, i.e., embryos termed viable for transfer and does who were not selected. For positive-implantation group, protein, called Jumonji (JARID2), was reported. This protein composes a histone methyltransferase complex called polycomb repressive complex 2 (PRC2), which modifies chromatin methylation to silence many embryonic patterning genes, acting as a negative regulator of cell proliferation signaling. This results with a restricted gene expression to an appropriate cell population that is essential for development, differentiation, and maintenance of cell fates.
\nIn the same study, the testis-specific gene 10 protein (TSGA10) was identified only in negative samples. TSGA10 is a perinuclear protein, and it was described to participate in actively dividing and fetal differentiating tissues in mice embryos.
\nKatz-Jaffe et al. reported the identification of heparin-binding epidermal growth factor EGF-like growth factor precursor (HB-EGF) [24]. This growth factor precursor belongs to the EGF family growth factors, and it is found in the membrane-anchored form (proHB-EGF) and the soluble form (sHB-EGF). The soluble form is produced from the proteolytic cleavage of proHB-EGF at the extracellular domain. Furthermore, soluble HB-EGF has been observed to be a growth stimulator, and it does significantly improve the blastocyst development and hatching when added to serum-free medium. However, other studies from an in vitro model system indicate that proHB-EGF might function in cell-to-cell signaling by a juxtacrine mechanism inhibiting growth activity [27]. Since this protein was identified in degenerating blastocytes, its upregulation might contribute to the lack of further development.
\nA cystatin-like precursor is needed for successful mammalian implantation for a controlled trophoblastic invasion of the maternal uterine epithelium. It must be available and functional [28] since this invasion involves the extracellular degradation of the uterine matrix by a variety of proteinases, and one of the crucial ones are cysteine proteinases. Cystatins are known to inhibit cysteine proteinases, and their upregulation will most probably contribute to failure of implantation of degenerating blastocysts. Therefore, it is not only of importance to identify these proteins, the possible biomarkers, but also to validate these findings in the human.
\nProteomics is a promising technology for the identification and validation of possible biomarkers for embryo selection in ART. As listed by Dyrlund et al., a growing list of secreted proteins has been identified that could further contribute to this field [21]. However, the challenge ahead of the research still includes the reliable and reproducible identification of a proteomics secretome signature. This signature shall be directly associated with embryo viability and the success of the procedure, i.e., successful pregnancy, and child’s birth. This is a very challenging task not only due to the complexity, heterogeneity, and diversity of human embryos but also because of irreproducibility of used culturing media and contaminant proteins therein.
\nAnother challenge for the clinical use of proteomics methods is the speed of the proteomics analysis. Sample preparation, measurement, and data analysis of a sample is currently not feasible within the time window needed for IVF. Current sample analysis methods require at least half a day for the fastest proteomics method available, which is too long. Nevertheless, proteomics methods can contribute to identification and validation of putative biomarkers, which once clinically confirmed, can be analyzed using other, faster, methods upon building corresponding antibodies.
\nCustomer Satisfaction is of paramount importance at IntechOpen and we take all complaints very seriously. Our Authors, their institutions, and other purchasers, if dissatisfied with the service provided, or the product purchased, can file a written complaint to IntechOpen, 5 Princes Gate Court, London, SW7 2QJ, UK or via the following e-mail address: info@intechopen.com.
',metaTitle:"Customer Complaints",metaDescription:"Our authors, their institutions and other purchasers, if unsatisfied with the service provided or the product purchased, can file a written complaint at IN TECH d.o.o offices at Janeza Trdine 9, 51000 Rijeka, Croatia, or via the following e-mail address: info@intechopen.com.",metaKeywords:null,canonicalURL:null,contentRaw:'[{"type":"htmlEditorComponent","content":"Receipt of complaints will be acknowledged in writing and Intech Limited will respond fully to concerns within 15 business days.
\\n\\nCustomers have the right to terminate the contract without giving any reason (written notice of termination). The deadline for said termination is fourteen (14) days from the date of receipt of goods. Returns are at the expense of the Customer and must be made within the fourteen (14) days from the date of the written notice of termination. Intech Limited will process refunds to the Customer without undue delay.
\\n\\nIn the event that the Publisher ships damaged or misbound copies of products, or duplicate or incorrect copies of the products are received by the Customer, the Publisher will accept returns at the Publisher's expense, provided notice of such damaged or incorrect shipment is given to the Publisher within fourteen (14) working days from the date of receipt.
\\n\\nPublishing errors, including but not limited to typographical errors, having no significant effect on the editorial content or design characteristics of the products, cannot be considered a reason for rejecting payment or, as the case may be, modifying the agreed price.
\\n\\nAt the Publisher's request, the customer should provide evidence of the damaged or incorrect shipment. The Publisher will refund or ship the ordered products without delays.
\\n"}]'},components:[{type:"htmlEditorComponent",content:"Receipt of complaints will be acknowledged in writing and Intech Limited will respond fully to concerns within 15 business days.
\n\nCustomers have the right to terminate the contract without giving any reason (written notice of termination). The deadline for said termination is fourteen (14) days from the date of receipt of goods. Returns are at the expense of the Customer and must be made within the fourteen (14) days from the date of the written notice of termination. Intech Limited will process refunds to the Customer without undue delay.
\n\nIn the event that the Publisher ships damaged or misbound copies of products, or duplicate or incorrect copies of the products are received by the Customer, the Publisher will accept returns at the Publisher's expense, provided notice of such damaged or incorrect shipment is given to the Publisher within fourteen (14) working days from the date of receipt.
\n\nPublishing errors, including but not limited to typographical errors, having no significant effect on the editorial content or design characteristics of the products, cannot be considered a reason for rejecting payment or, as the case may be, modifying the agreed price.
\n\nAt the Publisher's request, the customer should provide evidence of the damaged or incorrect shipment. The Publisher will refund or ship the ordered products without delays.
\n"}]},successStories:{items:[]},authorsAndEditors:{filterParams:{},profiles:[{id:"396",title:"Dr.",name:"Vedran",middleName:null,surname:"Kordic",slug:"vedran-kordic",fullName:"Vedran Kordic",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/396/images/7281_n.png",biography:"After obtaining his Master's degree in Mechanical Engineering he continued his education at the Vienna University of Technology where he obtained his PhD degree in 2004. He worked as a researcher at the Automation and Control Institute, Faculty of Electrical Engineering, Vienna University of Technology until 2008. His studies in robotics lead him not only to a PhD degree but also inspired him to co-found and build the International Journal of Advanced Robotic Systems - world's first Open Access journal in the field of robotics.",institutionString:null,institution:{name:"TU Wien",country:{name:"Austria"}}},{id:"441",title:"Ph.D.",name:"Jaekyu",middleName:null,surname:"Park",slug:"jaekyu-park",fullName:"Jaekyu Park",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/441/images/1881_n.jpg",biography:null,institutionString:null,institution:{name:"LG Corporation (South Korea)",country:{name:"Korea, South"}}},{id:"465",title:"Dr.",name:"Christian",middleName:null,surname:"Martens",slug:"christian-martens",fullName:"Christian Martens",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Rheinmetall (Germany)",country:{name:"Germany"}}},{id:"479",title:"Dr.",name:"Valentina",middleName:null,surname:"Colla",slug:"valentina-colla",fullName:"Valentina Colla",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/479/images/358_n.jpg",biography:null,institutionString:null,institution:{name:"Sant'Anna School of Advanced Studies",country:{name:"Italy"}}},{id:"494",title:"PhD",name:"Loris",middleName:null,surname:"Nanni",slug:"loris-nanni",fullName:"Loris Nanni",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/494/images/system/494.jpg",biography:"Loris Nanni received his Master Degree cum laude on June-2002 from the University of Bologna, and the April 26th 2006 he received his Ph.D. in Computer Engineering at DEIS, University of Bologna. On September, 29th 2006 he has won a post PhD fellowship from the university of Bologna (from October 2006 to October 2008), at the competitive examination he was ranked first in the industrial engineering area. He extensively served as referee for several international journals. He is author/coauthor of more than 100 research papers. He has been involved in some projects supported by MURST and European Community. His research interests include pattern recognition, bioinformatics, and biometric systems (fingerprint classification and recognition, signature verification, face recognition).",institutionString:null,institution:null},{id:"496",title:"Dr.",name:"Carlos",middleName:null,surname:"Leon",slug:"carlos-leon",fullName:"Carlos Leon",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"University of Seville",country:{name:"Spain"}}},{id:"512",title:"Dr.",name:"Dayang",middleName:null,surname:"Jawawi",slug:"dayang-jawawi",fullName:"Dayang Jawawi",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"University of Technology Malaysia",country:{name:"Malaysia"}}},{id:"528",title:"Dr.",name:"Kresimir",middleName:null,surname:"Delac",slug:"kresimir-delac",fullName:"Kresimir Delac",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/528/images/system/528.jpg",biography:"K. Delac received his B.Sc.E.E. degree in 2003 and is currentlypursuing a Ph.D. degree at the University of Zagreb, Faculty of Electrical Engineering andComputing. His current research interests are digital image analysis, pattern recognition andbiometrics.",institutionString:null,institution:{name:"University of Zagreb",country:{name:"Croatia"}}},{id:"557",title:"Dr.",name:"Andon",middleName:"Venelinov",surname:"Topalov",slug:"andon-topalov",fullName:"Andon Topalov",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/557/images/1927_n.jpg",biography:"Dr. Andon V. Topalov received the MSc degree in Control Engineering from the Faculty of Information Systems, Technologies, and Automation at Moscow State University of Civil Engineering (MGGU) in 1979. He then received his PhD degree in Control Engineering from the Department of Automation and Remote Control at Moscow State Mining University (MGSU), Moscow, in 1984. From 1985 to 1986, he was a Research Fellow in the Research Institute for Electronic Equipment, ZZU AD, Plovdiv, Bulgaria. In 1986, he joined the Department of Control Systems, Technical University of Sofia at the Plovdiv campus, where he is presently a Full Professor. He has held long-term visiting Professor/Scholar positions at various institutions in South Korea, Turkey, Mexico, Greece, Belgium, UK, and Germany. And he has coauthored one book and authored or coauthored more than 80 research papers in conference proceedings and journals. His current research interests are in the fields of intelligent control and robotics.",institutionString:null,institution:{name:"Technical University of Sofia",country:{name:"Bulgaria"}}},{id:"585",title:"Prof.",name:"Munir",middleName:null,surname:"Merdan",slug:"munir-merdan",fullName:"Munir Merdan",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/585/images/system/585.jpg",biography:"Munir Merdan received the M.Sc. degree in mechanical engineering from the Technical University of Sarajevo, Bosnia and Herzegovina, in 2001, and the Ph.D. degree in electrical engineering from the Vienna University of Technology, Vienna, Austria, in 2009.Since 2005, he has been at the Automation and Control Institute, Vienna University of Technology, where he is currently a Senior Researcher. His research interests include the application of agent technology for achieving agile control in the manufacturing environment.",institutionString:null,institution:null},{id:"605",title:"Prof",name:"Dil",middleName:null,surname:"Hussain",slug:"dil-hussain",fullName:"Dil Hussain",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/605/images/system/605.jpg",biography:"Dr. Dil Muhammad Akbar Hussain is a professor of Electronics Engineering & Computer Science at the Department of Energy Technology, Aalborg University Denmark. Professor Akbar has a Master degree in Digital Electronics from Govt. College University, Lahore Pakistan and a P-hD degree in Control Engineering from the School of Engineering and Applied Sciences, University of Sussex United Kingdom. Aalborg University has Two Satellite Campuses, one in Copenhagen (Aalborg University Copenhagen) and the other in Esbjerg (Aalborg University Esbjerg).\n· He is a member of prestigious IEEE (Institute of Electrical and Electronics Engineers), and IAENG (International Association of Engineers) organizations. \n· He is the chief Editor of the Journal of Software Engineering.\n· He is the member of the Editorial Board of International Journal of Computer Science and Software Technology (IJCSST) and International Journal of Computer Engineering and Information Technology. \n· He is also the Editor of Communication in Computer and Information Science CCIS-20 by Springer.\n· Reviewer For Many Conferences\nHe is the lead person in making collaboration agreements between Aalborg University and many universities of Pakistan, for which the MOU’s (Memorandum of Understanding) have been signed.\nProfessor Akbar is working in Academia since 1990, he started his career as a Lab demonstrator/TA at the University of Sussex. After finishing his P. hD degree in 1992, he served in the Industry as a Scientific Officer and continued his academic career as a visiting scholar for a number of educational institutions. In 1996 he joined National University of Science & Technology Pakistan (NUST) as an Associate Professor; NUST is one of the top few universities in Pakistan. In 1999 he joined an International Company Lineo Inc, Canada as Manager Compiler Group, where he headed the group for developing Compiler Tool Chain and Porting of Operating Systems for the BLACKfin processor. The processor development was a joint venture by Intel and Analog Devices. In 2002 Lineo Inc., was taken over by another company, so he joined Aalborg University Denmark as an Assistant Professor.\nProfessor Akbar has truly a multi-disciplined career and he continued his legacy and making progress in many areas of his interests both in teaching and research. He has contributed in stochastic estimation of control area especially, in the Multiple Target Tracking and Interactive Multiple Model (IMM) research, Ball & Beam Control Problem, Robotics, Levitation Control. He has contributed in developing Algorithms for Fingerprint Matching, Computer Vision and Face Recognition. He has been supervising Pattern Recognition, Formal Languages and Distributed Processing projects for several years. He has reviewed many books on Management, Computer Science. Currently, he is an active and permanent reviewer for many international conferences and symposia and the program committee member for many international conferences.\nIn teaching he has taught the core computer science subjects like, Digital Design, Real Time Embedded System Programming, Operating Systems, Software Engineering, Data Structures, Databases, Compiler Construction. In the Engineering side, Digital Signal Processing, Computer Architecture, Electronics Devices, Digital Filtering and Engineering Management.\nApart from his Academic Interest and activities he loves sport especially, Cricket, Football, Snooker and Squash. He plays cricket for Esbjerg city in the second division team as an opener wicket keeper batsman. He is a very good player of squash but has not played squash since his arrival in Denmark.",institutionString:null,institution:null},{id:"611",title:"Prof.",name:"T",middleName:null,surname:"Nagarajan",slug:"t-nagarajan",fullName:"T Nagarajan",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Universiti Teknologi Petronas",country:{name:"Malaysia"}}}],filtersByRegion:[{group:"region",caption:"North America",value:1,count:13404},{group:"region",caption:"Middle and South America",value:2,count:11681},{group:"region",caption:"Africa",value:3,count:4213},{group:"region",caption:"Asia",value:4,count:22421},{group:"region",caption:"Australia and Oceania",value:5,count:2020},{group:"region",caption:"Europe",value:6,count:33697}],offset:12,limit:12,total:135705},chapterEmbeded:{data:{}},editorApplication:{success:null,errors:{}},ofsBooks:{filterParams:{hasNoEditors:"0",sort:"dateEndThirdStepPublish",topicId:"16,19,25"},books:[{type:"book",id:"11027",title:"Basics of Hypoglycemia",subtitle:null,isOpenForSubmission:!0,hash:"98ebc1e36d02be82c204b8fd5d24f97a",slug:null,bookSignature:"Dr. Alok Raghav",coverURL:"https://cdn.intechopen.com/books/images_new/11027.jpg",editedByType:null,editors:[{id:"334465",title:"Dr.",name:"Alok",surname:"Raghav",slug:"alok-raghav",fullName:"Alok Raghav"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"11709",title:"Type 1 Diabetes Mellitus",subtitle:null,isOpenForSubmission:!0,hash:"cc0e61f864a2a8a9595f4975ce301f70",slug:null,bookSignature:"Dr. Shilpa Mehta",coverURL:"https://cdn.intechopen.com/books/images_new/11709.jpg",editedByType:null,editors:[{id:"342545",title:"Dr.",name:"Shilpa",surname:"Mehta",slug:"shilpa-mehta",fullName:"Shilpa Mehta"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"11814",title:"Liposomes - Recent Advances, New Perspectives and Applications",subtitle:null,isOpenForSubmission:!0,hash:"62d8542d18b8cddcf507f7948b2ae74b",slug:null,bookSignature:"Dr. Rajeev K. Tyagi",coverURL:"https://cdn.intechopen.com/books/images_new/11814.jpg",editedByType:null,editors:[{id:"269120",title:"Dr.",name:"Rajeev",surname:"Tyagi",slug:"rajeev-tyagi",fullName:"Rajeev Tyagi"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"11889",title:"Sexual Disorders and Dysfunctions",subtitle:null,isOpenForSubmission:!0,hash:"b988fda30a4e2364ee9d47e417bd0ba9",slug:null,bookSignature:"Dr. Dhastagir Sultan Sheriff",coverURL:"https://cdn.intechopen.com/books/images_new/11889.jpg",editedByType:null,editors:[{id:"167875",title:"Dr.",name:"Dhastagir Sultan",surname:"Sheriff",slug:"dhastagir-sultan-sheriff",fullName:"Dhastagir Sultan Sheriff"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"11867",title:"Echocardiography",subtitle:null,isOpenForSubmission:!0,hash:"d9159ce31733bf78cc2a79b18c225994",slug:null,bookSignature:"Dr. Gabriel Cismaru",coverURL:"https://cdn.intechopen.com/books/images_new/11867.jpg",editedByType:null,editors:[{id:"191888",title:"Dr.",name:"Gabriel",surname:"Cismaru",slug:"gabriel-cismaru",fullName:"Gabriel Cismaru"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"12107",title:"Contemporary Topics in Patient Safety - Volume 2",subtitle:null,isOpenForSubmission:!0,hash:"3fe674b93710773f0db746ca96d6e048",slug:null,bookSignature:"Dr. Philip Salen and Dr. Stanislaw P. Stawicki",coverURL:"https://cdn.intechopen.com/books/images_new/12107.jpg",editedByType:null,editors:[{id:"217603",title:"Dr.",name:"Philip",surname:"Salen",slug:"philip-salen",fullName:"Philip Salen"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"11591",title:"The Wounds of Our Mother Psychoanalysis - New Models for a Psychoanalysis in Crisis",subtitle:null,isOpenForSubmission:!0,hash:"c6a104ee38fec8d9ba8aa139a33003ce",slug:null,bookSignature:"Dr. Paolo Azzone",coverURL:"https://cdn.intechopen.com/books/images_new/11591.jpg",editedByType:null,editors:[{id:"324882",title:"Dr.",name:"Paolo",surname:"Azzone",slug:"paolo-azzone",fullName:"Paolo Azzone"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"11894",title:"Advances in Muscular Dystrophy Research - From Cellular and Molecular Basis to Therapies",subtitle:null,isOpenForSubmission:!0,hash:"8438d4a2b753a62d529eb68d6ade6597",slug:null,bookSignature:"Dr. Gisela Gaina",coverURL:"https://cdn.intechopen.com/books/images_new/11894.jpg",editedByType:null,editors:[{id:"242747",title:"Dr.",name:"Gisela",surname:"Gaina",slug:"gisela-gaina",fullName:"Gisela Gaina"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"11855",title:"Diabetic Foot - Recent Advances",subtitle:null,isOpenForSubmission:!0,hash:"9803b17d7d00c8eab822a0ab53d209b0",slug:null,bookSignature:"Dr. Alok Raghav",coverURL:"https://cdn.intechopen.com/books/images_new/11855.jpg",editedByType:null,editors:[{id:"334465",title:"Dr.",name:"Alok",surname:"Raghav",slug:"alok-raghav",fullName:"Alok Raghav"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"12105",title:"E-cigarettes and Health",subtitle:null,isOpenForSubmission:!0,hash:"3f372f37c421b5fc9a01f31341d478c7",slug:null,bookSignature:"Dr. Victor Hoe and Dr. Li Ping Wong",coverURL:"https://cdn.intechopen.com/books/images_new/12105.jpg",editedByType:null,editors:[{id:"267448",title:"Dr.",name:"Victor",surname:"Hoe",slug:"victor-hoe",fullName:"Victor Hoe"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"12101",title:"Healthcare Access - New Threats, New Approaches",subtitle:null,isOpenForSubmission:!0,hash:"da4020a2f408a62e168c093b7d5bdf4a",slug:null,bookSignature:"Prof. Ayse Emel Onal",coverURL:"https://cdn.intechopen.com/books/images_new/12101.jpg",editedByType:null,editors:[{id:"25840",title:"Prof.",name:"Ayse Emel",surname:"Onal",slug:"ayse-emel-onal",fullName:"Ayse Emel Onal"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"12104",title:"Viral Outbreaks - Global Trends and Perspectives",subtitle:null,isOpenForSubmission:!0,hash:"681a60ff84a29b9f72de9b662bab9c38",slug:null,bookSignature:"Prof. Shailendra K. Saxena",coverURL:"https://cdn.intechopen.com/books/images_new/12104.jpg",editedByType:null,editors:[{id:"158026",title:"Prof.",name:"Shailendra K.",surname:"Saxena",slug:"shailendra-k.-saxena",fullName:"Shailendra K. Saxena"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}}],filtersByTopic:[{group:"topic",caption:"Agricultural and Biological Sciences",value:5,count:22},{group:"topic",caption:"Biochemistry, Genetics and Molecular Biology",value:6,count:6},{group:"topic",caption:"Business, Management and Economics",value:7,count:4},{group:"topic",caption:"Chemistry",value:8,count:15},{group:"topic",caption:"Computer and Information Science",value:9,count:18},{group:"topic",caption:"Earth and Planetary Sciences",value:10,count:8},{group:"topic",caption:"Engineering",value:11,count:37},{group:"topic",caption:"Environmental Sciences",value:12,count:5},{group:"topic",caption:"Immunology and Microbiology",value:13,count:8},{group:"topic",caption:"Materials Science",value:14,count:15},{group:"topic",caption:"Mathematics",value:15,count:8},{group:"topic",caption:"Medicine",value:16,count:61},{group:"topic",caption:"Nanotechnology and Nanomaterials",value:17,count:2},{group:"topic",caption:"Neuroscience",value:18,count:2},{group:"topic",caption:"Pharmacology, Toxicology and Pharmaceutical Science",value:19,count:7},{group:"topic",caption:"Physics",value:20,count:5},{group:"topic",caption:"Psychology",value:21,count:5},{group:"topic",caption:"Robotics",value:22,count:2},{group:"topic",caption:"Social Sciences",value:23,count:8},{group:"topic",caption:"Veterinary Medicine and Science",value:25,count:2}],offset:12,limit:12,total:70},popularBooks:{featuredBooks:[{type:"book",id:"7827",title:"Interpersonal Relationships",subtitle:null,isOpenForSubmission:!1,hash:"ebf41f4d17c75010eb3294cc8cac3d47",slug:"interpersonal-relationships",bookSignature:"Martha Peaslee Levine",coverURL:"https://cdn.intechopen.com/books/images_new/7827.jpg",editors:[{id:"186919",title:"Dr.",name:"Martha",middleName:null,surname:"Peaslee Levine",slug:"martha-peaslee-levine",fullName:"Martha Peaslee Levine"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter"}},{type:"book",id:"10808",title:"Current Concepts in Dental Implantology",subtitle:"From Science to Clinical Research",isOpenForSubmission:!1,hash:"4af8830e463f89c57515c2da2b9777b0",slug:"current-concepts-in-dental-implantology-from-science-to-clinical-research",bookSignature:"Dragana Gabrić and Marko Vuletić",coverURL:"https://cdn.intechopen.com/books/images_new/10808.jpg",editors:[{id:"26946",title:"Prof.",name:"Dragana",middleName:null,surname:"Gabrić",slug:"dragana-gabric",fullName:"Dragana Gabrić"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter"}},{type:"book",id:"10796",title:"Extracellular Vesicles",subtitle:"Role in Diseases, Pathogenesis and Therapy",isOpenForSubmission:!1,hash:"eb5407fcf93baff7bca3fae5640153a2",slug:"extracellular-vesicles-role-in-diseases-pathogenesis-and-therapy",bookSignature:"Manash K. Paul",coverURL:"https://cdn.intechopen.com/books/images_new/10796.jpg",editors:[{id:"319365",title:"Assistant Prof.",name:"Manash K.",middleName:null,surname:"Paul",slug:"manash-k.-paul",fullName:"Manash K. Paul"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter"}},{type:"book",id:"10908",title:"Advances in Decision Making",subtitle:null,isOpenForSubmission:!1,hash:"126486f7f91e18e2e3539a32c38be7b1",slug:"advances-in-decision-making",bookSignature:"Fausto Pedro García Márquez",coverURL:"https://cdn.intechopen.com/books/images_new/10908.jpg",editors:[{id:"22844",title:"Prof.",name:"Fausto Pedro",middleName:null,surname:"García Márquez",slug:"fausto-pedro-garcia-marquez",fullName:"Fausto Pedro García Márquez"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter"}},{type:"book",id:"95",title:"Applications and Experiences of Quality Control",subtitle:null,isOpenForSubmission:!1,hash:"4bcb22b1eee68210a977a97d5a0f363a",slug:"applications-and-experiences-of-quality-control",bookSignature:"Ognyan Ivanov",coverURL:"https://cdn.intechopen.com/books/images_new/95.jpg",editors:[{id:"22230",title:"Prof.",name:"Ognyan",middleName:null,surname:"Ivanov",slug:"ognyan-ivanov",fullName:"Ognyan Ivanov"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter"}},{type:"book",id:"2160",title:"MATLAB",subtitle:"A Fundamental Tool for Scientific Computing and Engineering Applications - Volume 1",isOpenForSubmission:!1,hash:"dd9c658341fbd264ed4f8d9e6aa8ca29",slug:"matlab-a-fundamental-tool-for-scientific-computing-and-engineering-applications-volume-1",bookSignature:"Vasilios N. Katsikis",coverURL:"https://cdn.intechopen.com/books/images_new/2160.jpg",editors:[{id:"12289",title:"Prof.",name:"Vasilios",middleName:"N.",surname:"Katsikis",slug:"vasilios-katsikis",fullName:"Vasilios Katsikis"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter"}},{type:"book",id:"3560",title:"Advances in Landscape Architecture",subtitle:null,isOpenForSubmission:!1,hash:"a20614517ec5f7e91188fe8e42832138",slug:"advances-in-landscape-architecture",bookSignature:"Murat Özyavuz",coverURL:"https://cdn.intechopen.com/books/images_new/3560.jpg",editors:[{id:"93073",title:"Dr.",name:"Murat",middleName:null,surname:"Ozyavuz",slug:"murat-ozyavuz",fullName:"Murat Ozyavuz"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter"}},{type:"book",id:"10739",title:"Global Decline of Insects",subtitle:null,isOpenForSubmission:!1,hash:"543783652b9092962a8fa4bed38eeb17",slug:"global-decline-of-insects",bookSignature:"Hamadttu Abdel Farag El-Shafie",coverURL:"https://cdn.intechopen.com/books/images_new/10739.jpg",editors:[{id:"192142",title:"Dr.",name:"Hamadttu",middleName:null,surname:"Abdel Farag El-Shafie",slug:"hamadttu-abdel-farag-el-shafie",fullName:"Hamadttu Abdel Farag El-Shafie"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter"}},{type:"book",id:"10911",title:"Higher Education",subtitle:"New Approaches to Accreditation, Digitalization, and Globalization in the Age of Covid",isOpenForSubmission:!1,hash:"223a02337498e535e967174c1f648fbc",slug:"higher-education-new-approaches-to-accreditation-digitalization-and-globalization-in-the-age-of-covid",bookSignature:"Lee Waller and Sharon Waller",coverURL:"https://cdn.intechopen.com/books/images_new/10911.jpg",editors:[{id:"263301",title:"Dr.",name:"Lee",middleName:null,surname:"Waller",slug:"lee-waller",fullName:"Lee Waller"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter"}},{type:"book",id:"3568",title:"Recent Advances in Plant in vitro Culture",subtitle:null,isOpenForSubmission:!1,hash:"830bbb601742c85a3fb0eeafe1454c43",slug:"recent-advances-in-plant-in-vitro-culture",bookSignature:"Annarita Leva and Laura M. R. Rinaldi",coverURL:"https://cdn.intechopen.com/books/images_new/3568.jpg",editors:[{id:"142145",title:"Dr.",name:"Annarita",middleName:null,surname:"Leva",slug:"annarita-leva",fullName:"Annarita Leva"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter"}},{type:"book",id:"3737",title:"MATLAB",subtitle:"Modelling, Programming and Simulations",isOpenForSubmission:!1,hash:null,slug:"matlab-modelling-programming-and-simulations",bookSignature:"Emilson Pereira Leite",coverURL:"https://cdn.intechopen.com/books/images_new/3737.jpg",editors:[{id:"12051",title:"Prof.",name:"Emilson",middleName:null,surname:"Pereira Leite",slug:"emilson-pereira-leite",fullName:"Emilson Pereira Leite"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter"}},{type:"book",id:"1770",title:"Gel Electrophoresis",subtitle:"Principles and Basics",isOpenForSubmission:!1,hash:"279701f6c802cf02deef45103e0611ff",slug:"gel-electrophoresis-principles-and-basics",bookSignature:"Sameh Magdeldin",coverURL:"https://cdn.intechopen.com/books/images_new/1770.jpg",editors:[{id:"123648",title:"Dr.",name:"Sameh",middleName:null,surname:"Magdeldin",slug:"sameh-magdeldin",fullName:"Sameh Magdeldin"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter"}}],offset:12,limit:12,total:4802},hotBookTopics:{hotBooks:[],offset:0,limit:12,total:null},publish:{},publishingProposal:{success:null,errors:{}},books:{featuredBooks:[{type:"book",id:"7827",title:"Interpersonal Relationships",subtitle:null,isOpenForSubmission:!1,hash:"ebf41f4d17c75010eb3294cc8cac3d47",slug:"interpersonal-relationships",bookSignature:"Martha Peaslee Levine",coverURL:"https://cdn.intechopen.com/books/images_new/7827.jpg",publishedDate:"July 27th 2022",numberOfDownloads:7175,editors:[{id:"186919",title:"Dr.",name:"Martha",middleName:null,surname:"Peaslee Levine",slug:"martha-peaslee-levine",fullName:"Martha Peaslee Levine"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter"}},{type:"book",id:"10808",title:"Current Concepts in Dental Implantology",subtitle:"From Science to Clinical Research",isOpenForSubmission:!1,hash:"4af8830e463f89c57515c2da2b9777b0",slug:"current-concepts-in-dental-implantology-from-science-to-clinical-research",bookSignature:"Dragana Gabrić and Marko Vuletić",coverURL:"https://cdn.intechopen.com/books/images_new/10808.jpg",publishedDate:"July 27th 2022",numberOfDownloads:1981,editors:[{id:"26946",title:"Prof.",name:"Dragana",middleName:null,surname:"Gabrić",slug:"dragana-gabric",fullName:"Dragana Gabrić"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter"}},{type:"book",id:"10796",title:"Extracellular Vesicles",subtitle:"Role in Diseases, Pathogenesis and Therapy",isOpenForSubmission:!1,hash:"eb5407fcf93baff7bca3fae5640153a2",slug:"extracellular-vesicles-role-in-diseases-pathogenesis-and-therapy",bookSignature:"Manash K. Paul",coverURL:"https://cdn.intechopen.com/books/images_new/10796.jpg",publishedDate:"July 20th 2022",numberOfDownloads:2308,editors:[{id:"319365",title:"Assistant Prof.",name:"Manash K.",middleName:null,surname:"Paul",slug:"manash-k.-paul",fullName:"Manash K. Paul"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter"}},{type:"book",id:"10908",title:"Advances in Decision Making",subtitle:null,isOpenForSubmission:!1,hash:"126486f7f91e18e2e3539a32c38be7b1",slug:"advances-in-decision-making",bookSignature:"Fausto Pedro García Márquez",coverURL:"https://cdn.intechopen.com/books/images_new/10908.jpg",publishedDate:"July 27th 2022",numberOfDownloads:1473,editors:[{id:"22844",title:"Prof.",name:"Fausto Pedro",middleName:null,surname:"García Márquez",slug:"fausto-pedro-garcia-marquez",fullName:"Fausto Pedro García Márquez"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter"}},{type:"book",id:"95",title:"Applications and Experiences of Quality Control",subtitle:null,isOpenForSubmission:!1,hash:"4bcb22b1eee68210a977a97d5a0f363a",slug:"applications-and-experiences-of-quality-control",bookSignature:"Ognyan Ivanov",coverURL:"https://cdn.intechopen.com/books/images_new/95.jpg",publishedDate:"April 26th 2011",numberOfDownloads:318571,editors:[{id:"22230",title:"Prof.",name:"Ognyan",middleName:null,surname:"Ivanov",slug:"ognyan-ivanov",fullName:"Ognyan Ivanov"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter"}},{type:"book",id:"2160",title:"MATLAB",subtitle:"A Fundamental Tool for Scientific Computing and Engineering Applications - Volume 1",isOpenForSubmission:!1,hash:"dd9c658341fbd264ed4f8d9e6aa8ca29",slug:"matlab-a-fundamental-tool-for-scientific-computing-and-engineering-applications-volume-1",bookSignature:"Vasilios N. Katsikis",coverURL:"https://cdn.intechopen.com/books/images_new/2160.jpg",publishedDate:"September 26th 2012",numberOfDownloads:271836,editors:[{id:"12289",title:"Prof.",name:"Vasilios",middleName:"N.",surname:"Katsikis",slug:"vasilios-katsikis",fullName:"Vasilios Katsikis"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter"}},{type:"book",id:"3560",title:"Advances in Landscape Architecture",subtitle:null,isOpenForSubmission:!1,hash:"a20614517ec5f7e91188fe8e42832138",slug:"advances-in-landscape-architecture",bookSignature:"Murat Özyavuz",coverURL:"https://cdn.intechopen.com/books/images_new/3560.jpg",publishedDate:"July 1st 2013",numberOfDownloads:243450,editors:[{id:"93073",title:"Dr.",name:"Murat",middleName:null,surname:"Ozyavuz",slug:"murat-ozyavuz",fullName:"Murat Ozyavuz"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter"}},{type:"book",id:"10739",title:"Global Decline of Insects",subtitle:null,isOpenForSubmission:!1,hash:"543783652b9092962a8fa4bed38eeb17",slug:"global-decline-of-insects",bookSignature:"Hamadttu Abdel Farag El-Shafie",coverURL:"https://cdn.intechopen.com/books/images_new/10739.jpg",publishedDate:"July 20th 2022",numberOfDownloads:1582,editors:[{id:"192142",title:"Dr.",name:"Hamadttu",middleName:null,surname:"Abdel Farag El-Shafie",slug:"hamadttu-abdel-farag-el-shafie",fullName:"Hamadttu Abdel Farag El-Shafie"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter"}},{type:"book",id:"10911",title:"Higher Education",subtitle:"New Approaches to Accreditation, Digitalization, and Globalization in the Age of Covid",isOpenForSubmission:!1,hash:"223a02337498e535e967174c1f648fbc",slug:"higher-education-new-approaches-to-accreditation-digitalization-and-globalization-in-the-age-of-covid",bookSignature:"Lee Waller and Sharon Waller",coverURL:"https://cdn.intechopen.com/books/images_new/10911.jpg",publishedDate:"July 13th 2022",numberOfDownloads:2082,editors:[{id:"263301",title:"Dr.",name:"Lee",middleName:null,surname:"Waller",slug:"lee-waller",fullName:"Lee Waller"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter"}},{type:"book",id:"3568",title:"Recent Advances in Plant in vitro Culture",subtitle:null,isOpenForSubmission:!1,hash:"830bbb601742c85a3fb0eeafe1454c43",slug:"recent-advances-in-plant-in-vitro-culture",bookSignature:"Annarita Leva and Laura M. R. Rinaldi",coverURL:"https://cdn.intechopen.com/books/images_new/3568.jpg",publishedDate:"October 17th 2012",numberOfDownloads:256294,editors:[{id:"142145",title:"Dr.",name:"Annarita",middleName:null,surname:"Leva",slug:"annarita-leva",fullName:"Annarita Leva"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter"}}],latestBooks:[{type:"book",id:"8452",title:"Organizational Conflict",subtitle:"New Insights",isOpenForSubmission:!1,hash:"96bdaaba38a7850a7e7379aa5a505748",slug:"organizational-conflict-new-insights",bookSignature:"Josiane Fahed-Sreih",coverURL:"https://cdn.intechopen.com/books/images_new/8452.jpg",editedByType:"Edited by",publishedDate:"August 17th 2022",editors:[{id:"103784",title:"Dr.",name:"Josiane",middleName:null,surname:"Fahed-Sreih",slug:"josiane-fahed-sreih",fullName:"Josiane Fahed-Sreih"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"10778",title:"Model-Based Control Engineering",subtitle:"Recent Design and Implementations for Varied Applications",isOpenForSubmission:!1,hash:"e39a567d9b6d2a45d0a1d927362c9005",slug:"model-based-control-engineering-recent-design-and-implementations-for-varied-applications",bookSignature:"Umar Zakir Abdul Hamid and Ahmad `Athif Mohd Faudzi",coverURL:"https://cdn.intechopen.com/books/images_new/10778.jpg",editedByType:"Edited by",publishedDate:"August 17th 2022",editors:[{id:"268173",title:"Dr.",name:"Umar Zakir Abdul",middleName:null,surname:"Hamid",slug:"umar-zakir-abdul-hamid",fullName:"Umar Zakir Abdul Hamid"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"10780",title:"Current Trends in Orthodontics",subtitle:null,isOpenForSubmission:!1,hash:"badce0e23eb5176fd653b049d5295c0a",slug:"current-trends-in-orthodontics",bookSignature:"Farid Bourzgui",coverURL:"https://cdn.intechopen.com/books/images_new/10780.jpg",editedByType:"Edited by",publishedDate:"August 17th 2022",editors:[{id:"52177",title:"Prof.",name:"Farid",middleName:null,surname:"Bourzgui",slug:"farid-bourzgui",fullName:"Farid Bourzgui"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"10793",title:"Molecular Mechanisms in Cancer",subtitle:null,isOpenForSubmission:!1,hash:"3ed2817275edb3de6f5683602314706e",slug:"molecular-mechanisms-in-cancer",bookSignature:"Metin Budak and Rajamanickam Rajkumar",coverURL:"https://cdn.intechopen.com/books/images_new/10793.jpg",editedByType:"Edited by",publishedDate:"August 17th 2022",editors:[{id:"226275",title:"Ph.D.",name:"Metin",middleName:null,surname:"Budak",slug:"metin-budak",fullName:"Metin Budak"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"11308",title:"Selected Topics on Infant Feeding",subtitle:null,isOpenForSubmission:!1,hash:"213c3e403327a2919eca1dc5e82a0ec3",slug:"selected-topics-on-infant-feeding",bookSignature:"Isam Jaber AL-Zwaini and Haider Hadi AL-Musawi",coverURL:"https://cdn.intechopen.com/books/images_new/11308.jpg",editedByType:"Edited by",publishedDate:"August 17th 2022",editors:[{id:"30993",title:"Prof.",name:"Isam Jaber",middleName:null,surname:"Al-Zwaini",slug:"isam-jaber-al-zwaini",fullName:"Isam Jaber Al-Zwaini"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"10808",title:"Current Concepts in Dental Implantology",subtitle:"From Science to Clinical Research",isOpenForSubmission:!1,hash:"4af8830e463f89c57515c2da2b9777b0",slug:"current-concepts-in-dental-implantology-from-science-to-clinical-research",bookSignature:"Dragana Gabrić and Marko Vuletić",coverURL:"https://cdn.intechopen.com/books/images_new/10808.jpg",editedByType:"Edited by",publishedDate:"August 17th 2022",editors:[{id:"26946",title:"Prof.",name:"Dragana",middleName:null,surname:"Gabrić",slug:"dragana-gabric",fullName:"Dragana Gabrić"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"11331",title:"Secondary Metabolites",subtitle:"Trends and Reviews",isOpenForSubmission:!1,hash:"7d6274f42d5441e537c5fa744bc84523",slug:"secondary-metabolites-trends-and-reviews",bookSignature:"Ramasamy Vijayakumar and Suresh Selvapuram Sudalaimuthu Raja",coverURL:"https://cdn.intechopen.com/books/images_new/11331.jpg",editedByType:"Edited by",publishedDate:"August 17th 2022",editors:[{id:"176044",title:"Dr.",name:"Ramasamy",middleName:null,surname:"Vijayakumar",slug:"ramasamy-vijayakumar",fullName:"Ramasamy Vijayakumar"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"10820",title:"Data Clustering",subtitle:null,isOpenForSubmission:!1,hash:"086d299ffd05aacd2311c3ca4ebf0d3a",slug:"data-clustering",bookSignature:"Niansheng Tang",coverURL:"https://cdn.intechopen.com/books/images_new/10820.jpg",editedByType:"Edited by",publishedDate:"August 17th 2022",editors:[{id:"221831",title:"Prof.",name:"Niansheng",middleName:null,surname:"Tang",slug:"niansheng-tang",fullName:"Niansheng Tang"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"10827",title:"Oral Health Care",subtitle:"An Important Issue of the Modern Society",isOpenForSubmission:!1,hash:"9a0ceb9ced4598aea3f3723f6dc4ea04",slug:"oral-health-care-an-important-issue-of-the-modern-society",bookSignature:"Lavinia Cosmina Ardelean and Laura Cristina Rusu",coverURL:"https://cdn.intechopen.com/books/images_new/10827.jpg",editedByType:"Edited by",publishedDate:"August 17th 2022",editors:[{id:"180569",title:"Dr.",name:"Lavinia",middleName:null,surname:"Ardelean",slug:"lavinia-ardelean",fullName:"Lavinia Ardelean"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"11139",title:"Geochemistry and Mineral Resources",subtitle:null,isOpenForSubmission:!1,hash:"928cebbdce21d9b3f081267b24f12dfb",slug:"geochemistry-and-mineral-resources",bookSignature:"Hosam M. Saleh and Amal I. Hassan",coverURL:"https://cdn.intechopen.com/books/images_new/11139.jpg",editedByType:"Edited by",publishedDate:"August 17th 2022",editors:[{id:"144691",title:"Prof.",name:"Hosam M.",middleName:null,surname:"Saleh",slug:"hosam-m.-saleh",fullName:"Hosam M. Saleh"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}}]},subject:{topic:{id:"92",title:"Information and Knowledge Engineering",slug:"information-and-knowledge-engineering",parent:{id:"9",title:"Computer and Information Science",slug:"computer-and-information-science"},numberOfBooks:44,numberOfSeries:0,numberOfAuthorsAndEditors:891,numberOfWosCitations:745,numberOfCrossrefCitations:706,numberOfDimensionsCitations:1260,videoUrl:null,fallbackUrl:null,description:null},booksByTopicFilter:{topicId:"92",sort:"-publishedDate",limit:12,offset:0},booksByTopicCollection:[{type:"book",id:"10652",title:"Information Extraction and Object Tracking in Digital Video",subtitle:null,isOpenForSubmission:!1,hash:"d13718b2d986d058d55cf91e69bf21c0",slug:"information-extraction-and-object-tracking-in-digital-video",bookSignature:"Antonio José Ribeiro Neves and Francisco Javier Gallegos-Funes",coverURL:"https://cdn.intechopen.com/books/images_new/10652.jpg",editedByType:"Edited by",editors:[{id:"1177",title:"Prof.",name:"António",middleName:"J. R.",surname:"José Ribeiro Neves",slug:"antonio-jose-ribeiro-neves",fullName:"António José Ribeiro Neves"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"10693",title:"Open Data",subtitle:null,isOpenForSubmission:!1,hash:"0da9c2560358c1bc738e737aeb28f6a2",slug:"open-data",bookSignature:"Vijayalakshmi Kakulapati",coverURL:"https://cdn.intechopen.com/books/images_new/10693.jpg",editedByType:"Edited by",editors:[{id:"355072",title:"Prof.",name:"Vijayalakshmi",middleName:null,surname:"Kakulapati",slug:"vijayalakshmi-kakulapati",fullName:"Vijayalakshmi Kakulapati"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"10260",title:"Digital Service Platforms",subtitle:null,isOpenForSubmission:!1,hash:"11dab65781b3c4347022c56477311f46",slug:"digital-service-platforms",bookSignature:"Kyeong Kang",coverURL:"https://cdn.intechopen.com/books/images_new/10260.jpg",editedByType:"Edited by",editors:[{id:"2114",title:"Dr.",name:"Kyeong",middleName:null,surname:"Kang",slug:"kyeong-kang",fullName:"Kyeong Kang"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"9885",title:"Information Systems",subtitle:"Intelligent Information Processing Systems, Natural Language Processing, Affective Computing and Artificial Intelligence, and an Attempt to Build a Conversational Nursing Robot",isOpenForSubmission:!1,hash:"793751ee53f02ca84c8fe298a66208c9",slug:"information-systems-intelligent-information-processing-systems-natural-language-processing-affective-computing-and-artificial-intelligence-and-an-attempt-to-build-a-conversational-nursing-robot",bookSignature:"Kazuyuki Matsumoto",coverURL:"https://cdn.intechopen.com/books/images_new/9885.jpg",editedByType:"Edited by",editors:[{id:"195756",title:"Dr.",name:"Kazuyuki",middleName:null,surname:"Matsumoto",slug:"kazuyuki-matsumoto",fullName:"Kazuyuki Matsumoto"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"9975",title:"Digital Libraries",subtitle:"Advancing Open Science",isOpenForSubmission:!1,hash:"ac50e32e9acf00b04326c35b0b8f62f9",slug:"digital-libraries-advancing-open-science",bookSignature:"Sadia Vancauwenbergh",coverURL:"https://cdn.intechopen.com/books/images_new/9975.jpg",editedByType:"Edited by",editors:[{id:"235835",title:"Dr.",name:"Sadia",middleName:null,surname:"Vancauwenbergh",slug:"sadia-vancauwenbergh",fullName:"Sadia Vancauwenbergh"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"10208",title:"Data Integrity and Quality",subtitle:null,isOpenForSubmission:!1,hash:"f8ed9de4128cec41977b8759bedd7adc",slug:"data-integrity-and-quality",bookSignature:"Santhosh Kumar Balan",coverURL:"https://cdn.intechopen.com/books/images_new/10208.jpg",editedByType:"Edited by",editors:[{id:"330426",title:"Dr.",name:"B. Santhosh",middleName:null,surname:"Kumar",slug:"b.-santhosh-kumar",fullName:"B. Santhosh Kumar"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"8779",title:"Linked Open Data",subtitle:"Applications, Trends and Future Developments",isOpenForSubmission:!1,hash:"5860ff20764f7549ff218e9d5e112fef",slug:"linked-open-data-applications-trends-and-future-developments",bookSignature:"Kingsley Okoye",coverURL:"https://cdn.intechopen.com/books/images_new/8779.jpg",editedByType:"Edited by",editors:[{id:"219803",title:"Dr.",name:"Kingsley",middleName:null,surname:"Okoye",slug:"kingsley-okoye",fullName:"Kingsley Okoye"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"7780",title:"Ontological Analyses in Science, Technology and Informatics",subtitle:null,isOpenForSubmission:!1,hash:"72c8b15505d4716d94f299061496ef48",slug:"ontological-analyses-in-science-technology-and-informatics",bookSignature:"Andino Maseleno and Marini Othman",coverURL:"https://cdn.intechopen.com/books/images_new/7780.jpg",editedByType:"Edited by",editors:[{id:"219663",title:"Dr.",name:"Andino",middleName:null,surname:"Maseleno",slug:"andino-maseleno",fullName:"Andino Maseleno"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"8850",title:"Harnessing Knowledge, Innovation and Competence in Engineering of Mission Critical Systems",subtitle:null,isOpenForSubmission:!1,hash:"25ef9074be50f4e5c1f6cb7298e1b68d",slug:"harnessing-knowledge-innovation-and-competence-in-engineering-of-mission-critical-systems",bookSignature:"Ali G. Hessami",coverURL:"https://cdn.intechopen.com/books/images_new/8850.jpg",editedByType:"Edited by",editors:[{id:"108303",title:"Prof.",name:"Ali G.",middleName:null,surname:"Hessami",slug:"ali-g.-hessami",fullName:"Ali G. Hessami"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"8141",title:"Social Media and Machine Learning",subtitle:null,isOpenForSubmission:!1,hash:"155aa6c54dc411b5d2a1498f10f9417e",slug:"social-media-and-machine-learning",bookSignature:"Alberto Cano",coverURL:"https://cdn.intechopen.com/books/images_new/8141.jpg",editedByType:"Edited by",editors:[{id:"200724",title:"Dr.",name:"Alberto",middleName:null,surname:"Cano",slug:"alberto-cano",fullName:"Alberto Cano"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"9470",title:"Open Scientific Data",subtitle:"Why Choosing and Reusing the RIGHT DATA Matters",isOpenForSubmission:!1,hash:"898ef46a10e74ff18d1253b5200741ab",slug:"open-scientific-data-why-choosing-and-reusing-the-right-data-matters",bookSignature:"Vera J. Lipton",coverURL:"https://cdn.intechopen.com/books/images_new/9470.jpg",editedByType:"Authored by",editors:[{id:"307100",title:"Dr.",name:"Vera",middleName:null,surname:"Lipton",slug:"vera-lipton",fullName:"Vera Lipton"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"3",chapterContentType:"chapter",authoredCaption:"Authored by"}},{type:"book",id:"5844",title:"Ontology in Information Science",subtitle:null,isOpenForSubmission:!1,hash:"922bcfea0d27e7e004542ce3adca6d20",slug:"ontology-in-information-science",bookSignature:"Ciza Thomas",coverURL:"https://cdn.intechopen.com/books/images_new/5844.jpg",editedByType:"Edited by",editors:[{id:"43680",title:"Prof.",name:"Ciza",middleName:null,surname:"Thomas",slug:"ciza-thomas",fullName:"Ciza Thomas"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}}],booksByTopicTotal:44,seriesByTopicCollection:[],seriesByTopicTotal:0,mostCitedChapters:[{id:"38735",doi:"10.5772/51066",title:"Biomedical Named Entity Recognition: A Survey of Machine-Learning Tools",slug:"biomedical-named-entity-recognition-a-survey-of-machine-learning-tools",totalDownloads:5134,totalCrossrefCites:23,totalDimensionsCites:46,abstract:null,book:{id:"2746",slug:"theory-and-applications-for-advanced-text-mining",title:"Theory and Applications for Advanced Text Mining",fullTitle:"Theory and Applications for Advanced Text Mining"},signatures:"David Campos, Sérgio Matos and José Luís Oliveira",authors:[{id:"72193",title:"Dr.",name:"Jose Luis",middleName:null,surname:"Oliveira",slug:"jose-luis-oliveira",fullName:"Jose Luis Oliveira"},{id:"152991",title:"Dr.",name:"Sérgio",middleName:null,surname:"Matos",slug:"sergio-matos",fullName:"Sérgio Matos"},{id:"152992",title:"MSc.",name:"David",middleName:null,surname:"Campos",slug:"david-campos",fullName:"David Campos"}]},{id:"65993",doi:"10.5772/intechopen.84856",title:"Automatic Speech Emotion Recognition Using Machine Learning",slug:"automatic-speech-emotion-recognition-using-machine-learning",totalDownloads:4653,totalCrossrefCites:21,totalDimensionsCites:43,abstract:"This chapter presents a comparative study of speech emotion recognition (SER) systems. Theoretical definition, categorization of affective state and the modalities of emotion expression are presented. To achieve this study, an SER system, based on different classifiers and different methods for features extraction, is developed. Mel-frequency cepstrum coefficients (MFCC) and modulation spectral (MS) features are extracted from the speech signals and used to train different classifiers. Feature selection (FS) was applied in order to seek for the most relevant feature subset. Several machine learning paradigms were used for the emotion classification task. A recurrent neural network (RNN) classifier is used first to classify seven emotions. Their performances are compared later to multivariate linear regression (MLR) and support vector machines (SVM) techniques, which are widely used in the field of emotion recognition for spoken audio signals. Berlin and Spanish databases are used as the experimental data set. This study shows that for Berlin database all classifiers achieve an accuracy of 83% when a speaker normalization (SN) and a feature selection are applied to the features. For Spanish database, the best accuracy (94 %) is achieved by RNN classifier without SN and with FS.",book:{id:"8141",slug:"social-media-and-machine-learning",title:"Social Media and Machine Learning",fullTitle:"Social Media and Machine Learning"},signatures:"Leila Kerkeni, Youssef Serrestou, Mohamed Mbarki, Kosai Raoof, Mohamed Ali Mahjoub and Catherine Cleder",authors:[{id:"247090",title:"Ph.D. Student",name:"Leila",middleName:null,surname:"Kerkeni",slug:"leila-kerkeni",fullName:"Leila Kerkeni"}]},{id:"13173",doi:"10.5772/13222",title:"Glucose Prediction in Type 1 and Type 2 Diabetic Patients Using Data Driven Techniques",slug:"glucose-prediction-in-type-1-and-type-2-diabetic-patients-using-data-driven-techniques",totalDownloads:3665,totalCrossrefCites:0,totalDimensionsCites:27,abstract:null,book:{id:"1358",slug:"knowledge-oriented-applications-in-data-mining",title:"Knowledge-Oriented Applications in Data Mining",fullTitle:"Knowledge-Oriented Applications in Data Mining"},signatures:"Eleni I. Georga, Vasilios C. Protopappas and Dimitrios I. Fotiadis",authors:[{id:"14138",title:"Prof.",name:"Eleni",middleName:null,surname:"Georga",slug:"eleni-georga",fullName:"Eleni Georga"},{id:"16827",title:"Dr.",name:"Vasilios C.",middleName:null,surname:"Protopappas",slug:"vasilios-c.-protopappas",fullName:"Vasilios C. Protopappas"},{id:"16828",title:"Prof.",name:"Dimitrios",middleName:null,surname:"Fotiadis",slug:"dimitrios-fotiadis",fullName:"Dimitrios Fotiadis"}]},{id:"13162",doi:"10.5772/13683",title:"Data Mining Using RFM Analysis",slug:"data-mining-using-rfm-analysis",totalDownloads:21801,totalCrossrefCites:17,totalDimensionsCites:26,abstract:null,book:{id:"1358",slug:"knowledge-oriented-applications-in-data-mining",title:"Knowledge-Oriented Applications in Data Mining",fullTitle:"Knowledge-Oriented Applications in Data Mining"},signatures:"Derya Birant",authors:[{id:"15609",title:"Dr.",name:"Derya",middleName:null,surname:"Birant",slug:"derya-birant",fullName:"Derya Birant"}]},{id:"12352",doi:"10.5772/10361",title:"Swarm Robotics: An Extensive Research Review",slug:"swarm-robotics-an-extensive-research-review",totalDownloads:7229,totalCrossrefCites:3,totalDimensionsCites:25,abstract:null,book:{id:"5331",slug:"advanced-knowledge-application-in-practice",title:"Advanced Knowledge Application in Practice",fullTitle:"Advanced Knowledge Application in Practice"},signatures:"Yogeswaran M. and Ponnambalam S. G.",authors:null}],mostDownloadedChaptersLast30Days:[{id:"65993",title:"Automatic Speech Emotion Recognition Using Machine Learning",slug:"automatic-speech-emotion-recognition-using-machine-learning",totalDownloads:4654,totalCrossrefCites:21,totalDimensionsCites:43,abstract:"This chapter presents a comparative study of speech emotion recognition (SER) systems. Theoretical definition, categorization of affective state and the modalities of emotion expression are presented. To achieve this study, an SER system, based on different classifiers and different methods for features extraction, is developed. Mel-frequency cepstrum coefficients (MFCC) and modulation spectral (MS) features are extracted from the speech signals and used to train different classifiers. Feature selection (FS) was applied in order to seek for the most relevant feature subset. Several machine learning paradigms were used for the emotion classification task. A recurrent neural network (RNN) classifier is used first to classify seven emotions. Their performances are compared later to multivariate linear regression (MLR) and support vector machines (SVM) techniques, which are widely used in the field of emotion recognition for spoken audio signals. Berlin and Spanish databases are used as the experimental data set. This study shows that for Berlin database all classifiers achieve an accuracy of 83% when a speaker normalization (SN) and a feature selection are applied to the features. For Spanish database, the best accuracy (94 %) is achieved by RNN classifier without SN and with FS.",book:{id:"8141",slug:"social-media-and-machine-learning",title:"Social Media and Machine Learning",fullTitle:"Social Media and Machine Learning"},signatures:"Leila Kerkeni, Youssef Serrestou, Mohamed Mbarki, Kosai Raoof, Mohamed Ali Mahjoub and Catherine Cleder",authors:[{id:"247090",title:"Ph.D. Student",name:"Leila",middleName:null,surname:"Kerkeni",slug:"leila-kerkeni",fullName:"Leila Kerkeni"}]},{id:"37307",title:"Cyber Security",slug:"cybersecurity-in-the-real-world-implications-and-applications",totalDownloads:4094,totalCrossrefCites:0,totalDimensionsCites:0,abstract:null,book:{id:"1923",slug:"security-enhanced-applications-for-information-systems",title:"Security Enhanced Applications for Information Systems",fullTitle:"Security Enhanced Applications for Information Systems"},signatures:"Barry Lunt, Dale Rowe and Joseph Ekstrom",authors:[{id:"110690",title:"Prof.",name:"Barry",middleName:null,surname:"Lunt",slug:"barry-lunt",fullName:"Barry Lunt"},{id:"124554",title:"Prof.",name:"Joseph",middleName:null,surname:"Ekstrom",slug:"joseph-ekstrom",fullName:"Joseph Ekstrom"},{id:"124555",title:"Prof.",name:"Dale",middleName:null,surname:"Rowe",slug:"dale-rowe",fullName:"Dale Rowe"}]},{id:"48924",title:"Effective eLearning and eTeaching — A Theoretical Model",slug:"effective-elearning-and-eteaching-a-theoretical-model",totalDownloads:3076,totalCrossrefCites:7,totalDimensionsCites:10,abstract:"Distance learning is increasingly becoming an option for learners that were previously denied higher education opportunities due to elitist systems, cost, academic preparation, or personal circumstances. It is also a means to help nations meet goals to increase the percentage of individuals with post-secondary education in order to address workforce needs. However, learners and instructors often have concerns with their ability to be successful in a distance learning environment. This chapter presents a theoretical model for eLearning and eTeaching aimed at helping learners and instructors successfully navigate distance learning courses. Examples of course activities corresponding to the model components are shared. A qualitative analysis of learner self-reflections demonstrates the efficacy of the model in terms of increased autonomy, self-regulation, and targeted skills.",book:{id:"4792",slug:"e-learning-instructional-design-organizational-strategy-and-management",title:"E-Learning",fullTitle:"E-Learning - Instructional Design, Organizational Strategy and Management"},signatures:"Maureen Snow Andrade",authors:[{id:"96902",title:"Dr.",name:"Maureen",middleName:null,surname:"Snow Andrade",slug:"maureen-snow-andrade",fullName:"Maureen Snow Andrade"}]},{id:"37322",title:"Mobile System Applied to Species Distribution Modelling",slug:"mobile-system-applied-to-species-distribution-modelling",totalDownloads:2028,totalCrossrefCites:0,totalDimensionsCites:0,abstract:null,book:{id:"2505",slug:"innovative-information-systems-modelling-techniques",title:"Innovative Information Systems Modelling Techniques",fullTitle:"Innovative Information Systems Modelling Techniques"},signatures:"Álvaro Silva, Pedro Corrêa and Carlos Valêncio",authors:[{id:"116016",title:"MSc.",name:"Alvaro",middleName:"Fagner Rodrigues Da",surname:"Silva",slug:"alvaro-silva",fullName:"Alvaro Silva"},{id:"116660",title:"Dr.",name:"Pedro Luiz",middleName:null,surname:"Pizzigatti Correa",slug:"pedro-luiz-pizzigatti-correa",fullName:"Pedro Luiz Pizzigatti Correa"},{id:"140676",title:"Dr.",name:"Carlos Roberto",middleName:null,surname:"Valêncio",slug:"carlos-roberto-valencio",fullName:"Carlos Roberto Valêncio"}]},{id:"38450",title:"Microwave Antenna Performance Metrics",slug:"microwave-antenna-performance-metrics",totalDownloads:15117,totalCrossrefCites:0,totalDimensionsCites:0,abstract:null,book:{id:"2203",slug:"data-acquisition-applications",title:"Data Acquisition Applications",fullTitle:"Data Acquisition Applications"},signatures:"Paul Osaretin Otasowie",authors:[{id:"145221",title:"Dr.",name:"Paul",middleName:"Osaretin",surname:"Otasowie",slug:"paul-otasowie",fullName:"Paul Otasowie"}]}],onlineFirstChaptersFilter:{topicId:"92",limit:6,offset:0},onlineFirstChaptersCollection:[{id:"82418",title:"Bayesian Networks for Decision Support in Emergency Response: A Model for Missing Person Investigations",slug:"bayesian-networks-for-decision-support-in-emergency-response-a-model-for-missing-person-investigatio",totalDownloads:10,totalDimensionsCites:0,doi:"10.5772/intechopen.105047",abstract:"The successful operation of Emergency services (Police, Fire, Medical Emergency) relies heavily upon Information Systems and particularly Decision Support Systems. Missing person cases consume resources from the already overstretched resources of Police Forces. Such cases predominantly come from at-risk groups such as children in care, people suffering from depression, or elderly people suffering from dementia. This chapter reviews current practices used for missing person cases and describes a decision support model based on Bayesian networks.",book:{id:"11068",title:"Contemporary Issues in Information Systems - A Global Perspective",coverURL:"https://cdn.intechopen.com/books/images_new/11068.jpg"},signatures:"Denis Reilly"},{id:"77251",title:"The Applicability of Internet Voting in Africa",slug:"the-applicability-of-internet-voting-in-africa",totalDownloads:102,totalDimensionsCites:0,doi:"10.5772/intechopen.98576",abstract:"The covid-19 pandemic has brought about new ways of conducting business through the use of Information Communication Technologies and elections have not been spared either. Internet voting is another form of strengthening democracy through the use of Information Communication Technologies. Africa lags in the implementation of electronic voting, especially Internet voting. This chapter applied a critical socio-technical analysis that analyses factors that influence the applicability of Internet voting within the African context. The researcher applied desktop research which included 30 journals to gather data from the Internet and other documentation sources. The findings reveal that decision-makers can partially implement Internet voting in some of the countries in Africa like Kenya, Libya, Nigeria, Morocco, Mauritius, Tunisia, and Seychelles. To successfully implement Internet voting, the decision-makers in African nations have to fully invest in the Information Communication Technology infrastructure, provide the necessary security, legislation and carry out intensive voter education to build trust among voters.",book:{id:"11068",title:"Contemporary Issues in Information Systems - A Global Perspective",coverURL:"https://cdn.intechopen.com/books/images_new/11068.jpg"},signatures:"Paul Sambo"},{id:"79473",title:"Interdisciplinary Integrated Tools to Problem Solving 2.0",slug:"interdisciplinary-integrated-tools-to-problem-solving-2-0",totalDownloads:54,totalDimensionsCites:0,doi:"10.5772/intechopen.101456",abstract:"Everyone understands the events they witness or read about according to their mental models, and that is one of the main reasons there are a lot of disagreements at workplaces and between friends and families. Considering this situation, plus the difficulty that most people face when trying to conceptualize problems, I suggest a course that includes series methodologies, working synergistically to deal with this problem that goes from understanding the differences between people to test multiple hypotheses and planning the solution implementation. Since 2014, I have been teaching with some colleagues this tool in the format of a short course that articulates systems thinking, mapping studies, information quality, and competing hypotheses. This course has been presented often not only in Argentina and also in Peru with great success. Considering the pandemic situation, since 2020, it has been taught virtually. The latest modification to the original structure of the course was the incorporation of the Gantt chart to design the implementation of the solution found. This paper will present our course and the logic behind it, its outcomes, and how it evolved with the different iterations.",book:{id:"11068",title:"Contemporary Issues in Information Systems - A Global Perspective",coverURL:"https://cdn.intechopen.com/books/images_new/11068.jpg"},signatures:"Maria J. Espona"},{id:"76540",title:"Basic Concepts of Information Systems",slug:"basic-concepts-of-information-systems",totalDownloads:323,totalDimensionsCites:0,doi:"10.5772/intechopen.97644",abstract:"This chapter covers the basic concepts of the information systems (IS) field to prepare the reader to quickly approach the book’s other chapters: the Definition of information, the notion of system, and, more particularly, information systems. We also discuss the typology of IS according to the managerial level and decision-making in the IS. Furthermore, we describe information systems applications covering functional areas and focusing on the execution of business processes across the enterprise, including all management levels. We briefly discuss the aspects related to IS security that ensure the protection and integrity of information. We continue our exploration by presenting several metrics, mainly financial, to assess the added value of IS in companies. Next, we present a brief description of a very fashionable approach to make the information system evolve in all coherence, which is the urbanization of IS. We conclude this chapter with some IS challenges focusing on the leading causes of IS implementation’s failure and success.",book:{id:"11068",title:"Contemporary Issues in Information Systems - A Global Perspective",coverURL:"https://cdn.intechopen.com/books/images_new/11068.jpg"},signatures:"Leila Zemmouchi-Ghomari"},{id:"77302",title:"Clinical Pathway for Improving Quality Service and Cost Containtment in Hospital",slug:"clinical-pathway-for-improving-quality-service-and-cost-containtment-in-hospital",totalDownloads:112,totalDimensionsCites:0,doi:"10.5772/intechopen.98596",abstract:"The explanation begins with the Clinical Pathway in Hospital which describes how the Clinical Pathway is used in relation to 2 things: Components-Linkages and Step-Problems-Optimal Solution, followed by Linkages Clinical Pathway with Quality Improvement and Cost Containment, which describes the relationship of each. Followed by the Clinical Pathway for Service Quality: which consists of: (1) Clinical Pathway for Service Quality, (2) Patient Safety for Service Quality Improvement, (3) The role of alogarithm, thereby clarifying the form of clinical pathways in quality improvement efforts that ensure service improvement by still maintain the quality that is maintained during the cost containment. The Clinical Pathway in Cost Containment describes the roles of: (1) Link of Components, (2) Procedure, (3) Unit Cost, so that cost containment efforts can be made in the form of cost containment optimally while maintaining quality does not need to decrease. Clinical Pathway in New Era is a newly developed algorithm related to current and future conditions. This is related to: (1) New Era in Pandemic Covid-19, (2) Clinical Pathway in Non Curative Service, (3) Clinical Pathway in Technology Services, (4) Clinical Pathway in Technological Rerelated while continuing to carry out quality improvement and cost containment simultaneously. Concluton: clinical pathway in hospital can be used as a system for Quality Improvement and Cost Containment, related to New Era in Pandemic Covid-19, Non Curative Service, Technology Services and Technological Rerelated.",book:{id:"11068",title:"Contemporary Issues in Information Systems - A Global Perspective",coverURL:"https://cdn.intechopen.com/books/images_new/11068.jpg"},signatures:"Boy Subirosa Sabarguna"}],onlineFirstChaptersTotal:5},preDownload:{success:null,errors:{}},subscriptionForm:{success:null,errors:{}},aboutIntechopen:{},privacyPolicy:{},peerReviewing:{},howOpenAccessPublishingWithIntechopenWorks:{},sponsorshipBooks:{sponsorshipBooks:[],offset:8,limit:8,total:0},allSeries:{pteSeriesList:[{id:"14",title:"Artificial Intelligence",numberOfPublishedBooks:11,numberOfPublishedChapters:91,numberOfOpenTopics:6,numberOfUpcomingTopics:0,issn:"2633-1403",doi:"10.5772/intechopen.79920",isOpenForSubmission:!0},{id:"7",title:"Biomedical Engineering",numberOfPublishedBooks:12,numberOfPublishedChapters:108,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2631-5343",doi:"10.5772/intechopen.71985",isOpenForSubmission:!0}],lsSeriesList:[{id:"11",title:"Biochemistry",numberOfPublishedBooks:33,numberOfPublishedChapters:333,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2632-0983",doi:"10.5772/intechopen.72877",isOpenForSubmission:!0},{id:"25",title:"Environmental Sciences",numberOfPublishedBooks:1,numberOfPublishedChapters:19,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2754-6713",doi:"10.5772/intechopen.100362",isOpenForSubmission:!0},{id:"10",title:"Physiology",numberOfPublishedBooks:14,numberOfPublishedChapters:145,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2631-8261",doi:"10.5772/intechopen.72796",isOpenForSubmission:!0}],hsSeriesList:[{id:"3",title:"Dentistry",numberOfPublishedBooks:11,numberOfPublishedChapters:144,numberOfOpenTopics:2,numberOfUpcomingTopics:0,issn:"2631-6218",doi:"10.5772/intechopen.71199",isOpenForSubmission:!0},{id:"6",title:"Infectious Diseases",numberOfPublishedBooks:13,numberOfPublishedChapters:125,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2631-6188",doi:"10.5772/intechopen.71852",isOpenForSubmission:!0},{id:"13",title:"Veterinary Medicine and Science",numberOfPublishedBooks:11,numberOfPublishedChapters:113,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2632-0517",doi:"10.5772/intechopen.73681",isOpenForSubmission:!0}],sshSeriesList:[{id:"22",title:"Business, Management and Economics",numberOfPublishedBooks:1,numberOfPublishedChapters:23,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2753-894X",doi:"10.5772/intechopen.100359",isOpenForSubmission:!0},{id:"23",title:"Education and Human Development",numberOfPublishedBooks:0,numberOfPublishedChapters:12,numberOfOpenTopics:1,numberOfUpcomingTopics:1,issn:null,doi:"10.5772/intechopen.100360",isOpenForSubmission:!0},{id:"24",title:"Sustainable Development",numberOfPublishedBooks:1,numberOfPublishedChapters:19,numberOfOpenTopics:5,numberOfUpcomingTopics:0,issn:"2753-6580",doi:"10.5772/intechopen.100361",isOpenForSubmission:!0}],testimonialsList:[{id:"13",text:"The collaboration with and support of the technical staff of IntechOpen is fantastic. The whole process of submitting an article and editing of the submitted article goes extremely smooth and fast, the number of reads and downloads of chapters is high, and the contributions are also frequently cited.",author:{id:"55578",name:"Antonio",surname:"Jurado-Navas",institutionString:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRisIQAS/Profile_Picture_1626166543950",slug:"antonio-jurado-navas",institution:{id:"720",name:"University of Malaga",country:{id:null,name:"Spain"}}}},{id:"6",text:"It is great to work with the IntechOpen to produce a worthwhile collection of research that also becomes a great educational resource and guide for future research endeavors.",author:{id:"259298",name:"Edward",surname:"Narayan",institutionString:null,profilePictureURL:"https://mts.intechopen.com/storage/users/259298/images/system/259298.jpeg",slug:"edward-narayan",institution:{id:"3",name:"University of Queensland",country:{id:null,name:"Australia"}}}}]},series:{item:{id:"11",title:"Biochemistry",doi:"10.5772/intechopen.72877",issn:"2632-0983",scope:"Biochemistry, the study of chemical transformations occurring within living organisms, impacts all areas of life sciences, from molecular crystallography and genetics to ecology, medicine, and population biology. Biochemistry examines macromolecules - proteins, nucleic acids, carbohydrates, and lipids – and their building blocks, structures, functions, and interactions. Much of biochemistry is devoted to enzymes, proteins that catalyze chemical reactions, enzyme structures, mechanisms of action and their roles within cells. Biochemistry also studies small signaling molecules, coenzymes, inhibitors, vitamins, and hormones, which play roles in life processes. Biochemical experimentation, besides coopting classical chemistry methods, e.g., chromatography, adopted new techniques, e.g., X-ray diffraction, electron microscopy, NMR, radioisotopes, and developed sophisticated microbial genetic tools, e.g., auxotroph mutants and their revertants, fermentation, etc. More recently, biochemistry embraced the ‘big data’ omics systems. Initial biochemical studies have been exclusively analytic: dissecting, purifying, and examining individual components of a biological system; in the apt words of Efraim Racker (1913 –1991), “Don’t waste clean thinking on dirty enzymes.” Today, however, biochemistry is becoming more agglomerative and comprehensive, setting out to integrate and describe entirely particular biological systems. The ‘big data’ metabolomics can define the complement of small molecules, e.g., in a soil or biofilm sample; proteomics can distinguish all the comprising proteins, e.g., serum; metagenomics can identify all the genes in a complex environment, e.g., the bovine rumen. This Biochemistry Series will address the current research on biomolecules and the emerging trends with great promise.",coverUrl:"https://cdn.intechopen.com/series/covers/11.jpg",latestPublicationDate:"August 17th, 2022",hasOnlineFirst:!0,numberOfPublishedBooks:33,editor:{id:"31610",title:"Dr.",name:"Miroslav",middleName:null,surname:"Blumenberg",slug:"miroslav-blumenberg",fullName:"Miroslav Blumenberg",profilePictureURL:"https://mts.intechopen.com/storage/users/31610/images/system/31610.jpg",biography:"Miroslav Blumenberg, Ph.D., was born in Subotica and received his BSc in Belgrade, Yugoslavia. He completed his Ph.D. at MIT in Organic Chemistry; he followed up his Ph.D. with two postdoctoral study periods at Stanford University. Since 1983, he has been a faculty member of the RO Perelman Department of Dermatology, NYU School of Medicine, where he is codirector of a training grant in cutaneous biology. Dr. Blumenberg’s research is focused on the epidermis, expression of keratin genes, transcription profiling, keratinocyte differentiation, inflammatory diseases and cancers, and most recently the effects of the microbiome on the skin. He has published more than 100 peer-reviewed research articles and graduated numerous Ph.D. and postdoctoral students.",institutionString:null,institution:{name:"New York University Langone Medical Center",institutionURL:null,country:{name:"United States of America"}}},editorTwo:null,editorThree:null},subseries:{paginationCount:6,paginationItems:[{id:"22",title:"Applied Intelligence",coverUrl:"https://cdn.intechopen.com/series_topics/covers/22.jpg",isOpenForSubmission:!0,editor:{id:"27170",title:"Prof.",name:"Carlos",middleName:"M.",surname:"Travieso-Gonzalez",slug:"carlos-travieso-gonzalez",fullName:"Carlos Travieso-Gonzalez",profilePictureURL:"https://mts.intechopen.com/storage/users/27170/images/system/27170.jpeg",biography:"Carlos M. Travieso-González received his MSc degree in Telecommunication Engineering at Polytechnic University of Catalonia (UPC), Spain in 1997, and his Ph.D. degree in 2002 at the University of Las Palmas de Gran Canaria (ULPGC-Spain). He is a full professor of signal processing and pattern recognition and is head of the Signals and Communications Department at ULPGC, teaching from 2001 on subjects on signal processing and learning theory. His research lines are biometrics, biomedical signals and images, data mining, classification system, signal and image processing, machine learning, and environmental intelligence. He has researched in 52 international and Spanish research projects, some of them as head researcher. He is co-author of 4 books, co-editor of 27 proceedings books, guest editor for 8 JCR-ISI international journals, and up to 24 book chapters. He has over 450 papers published in international journals and conferences (81 of them indexed on JCR – ISI - Web of Science). He has published seven patents in the Spanish Patent and Trademark Office. He has been a supervisor on 8 Ph.D. theses (11 more are under supervision), and 130 master theses. He is the founder of The IEEE IWOBI conference series and the president of its Steering Committee, as well as the founder of both the InnoEducaTIC and APPIS conference series. He is an evaluator of project proposals for the European Union (H2020), Medical Research Council (MRC, UK), Spanish Government (ANECA, Spain), Research National Agency (ANR, France), DAAD (Germany), Argentinian Government, and the Colombian Institutions. He has been a reviewer in different indexed international journals (<70) and conferences (<250) since 2001. He has been a member of the IASTED Technical Committee on Image Processing from 2007 and a member of the IASTED Technical Committee on Artificial Intelligence and Expert Systems from 2011. \n\nHe has held the general chair position for the following: ACM-APPIS (2020, 2021), IEEE-IWOBI (2019, 2020 and 2020), A PPIS (2018, 2019), IEEE-IWOBI (2014, 2015, 2017, 2018), InnoEducaTIC (2014, 2017), IEEE-INES (2013), NoLISP (2011), JRBP (2012), and IEEE-ICCST (2005)\n\nHe is an associate editor of the Computational Intelligence and Neuroscience Journal (Hindawi – Q2 JCR-ISI). He was vice dean from 2004 to 2010 in the Higher Technical School of Telecommunication Engineers at ULPGC and the vice dean of Graduate and Postgraduate Studies from March 2013 to November 2017. He won the “Catedra Telefonica” Awards in Modality of Knowledge Transfer, 2017, 2018, and 2019 editions, and awards in Modality of COVID Research in 2020.\n\nPublic References:\nResearcher ID http://www.researcherid.com/rid/N-5967-2014\nORCID https://orcid.org/0000-0002-4621-2768 \nScopus Author ID https://www.scopus.com/authid/detail.uri?authorId=6602376272\nScholar Google https://scholar.google.es/citations?user=G1ks9nIAAAAJ&hl=en \nResearchGate https://www.researchgate.net/profile/Carlos_Travieso",institutionString:null,institution:{name:"University of Las Palmas de Gran Canaria",institutionURL:null,country:{name:"Spain"}}},editorTwo:null,editorThree:null},{id:"23",title:"Computational Neuroscience",coverUrl:"https://cdn.intechopen.com/series_topics/covers/23.jpg",isOpenForSubmission:!0,editor:{id:"14004",title:"Dr.",name:"Magnus",middleName:null,surname:"Johnsson",slug:"magnus-johnsson",fullName:"Magnus Johnsson",profilePictureURL:"https://mts.intechopen.com/storage/users/14004/images/system/14004.png",biography:"Dr Magnus Johnsson is a cross-disciplinary scientist, lecturer, scientific editor and AI/machine learning consultant from Sweden. \n\nHe is currently at Malmö University in Sweden, but also held positions at Lund University in Sweden and at Moscow Engineering Physics Institute. \nHe holds editorial positions at several international scientific journals and has served as a scientific editor for books and special journal issues. \nHis research interests are wide and include, but are not limited to, autonomous systems, computer modeling, artificial neural networks, artificial intelligence, cognitive neuroscience, cognitive robotics, cognitive architectures, cognitive aids and the philosophy of mind. \n\nDr. Johnsson has experience from working in the industry and he has a keen interest in the application of neural networks and artificial intelligence to fields like industry, finance, and medicine. \n\nWeb page: www.magnusjohnsson.se",institutionString:null,institution:{name:"Malmö University",institutionURL:null,country:{name:"Sweden"}}},editorTwo:null,editorThree:null},{id:"24",title:"Computer Vision",coverUrl:"https://cdn.intechopen.com/series_topics/covers/24.jpg",isOpenForSubmission:!0,editor:{id:"294154",title:"Prof.",name:"George",middleName:null,surname:"Papakostas",slug:"george-papakostas",fullName:"George Papakostas",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002hYaGbQAK/Profile_Picture_1624519712088",biography:"George A. Papakostas has received a diploma in Electrical and Computer Engineering in 1999 and the M.Sc. and Ph.D. degrees in Electrical and Computer Engineering in 2002 and 2007, respectively, from the Democritus University of Thrace (DUTH), Greece. Dr. Papakostas serves as a Tenured Full Professor at the Department of Computer Science, International Hellenic University, Greece. Dr. Papakostas has 10 years of experience in large-scale systems design as a senior software engineer and technical manager, and 20 years of research experience in the field of Artificial Intelligence. Currently, he is the Head of the “Visual Computing” division of HUman-MAchines INteraction Laboratory (HUMAIN-Lab) and the Director of the MPhil program “Advanced Technologies in Informatics and Computers” hosted by the Department of Computer Science, International Hellenic University. He has (co)authored more than 150 publications in indexed journals, international conferences and book chapters, 1 book (in Greek), 3 edited books, and 5 journal special issues. His publications have more than 2100 citations with h-index 27 (GoogleScholar). His research interests include computer/machine vision, machine learning, pattern recognition, computational intelligence. \nDr. Papakostas served as a reviewer in numerous journals, as a program\ncommittee member in international conferences and he is a member of the IAENG, MIR Labs, EUCogIII, INSTICC and the Technical Chamber of Greece (TEE).",institutionString:null,institution:{name:"International Hellenic University",institutionURL:null,country:{name:"Greece"}}},editorTwo:null,editorThree:null},{id:"25",title:"Evolutionary Computation",coverUrl:"https://cdn.intechopen.com/series_topics/covers/25.jpg",isOpenForSubmission:!0,editor:{id:"136112",title:"Dr.",name:"Sebastian",middleName:null,surname:"Ventura Soto",slug:"sebastian-ventura-soto",fullName:"Sebastian Ventura Soto",profilePictureURL:"https://mts.intechopen.com/storage/users/136112/images/system/136112.png",biography:"Sebastian Ventura is a Spanish researcher, a full professor with the Department of Computer Science and Numerical Analysis, University of Córdoba. Dr Ventura also holds the positions of Affiliated Professor at Virginia Commonwealth University (Richmond, USA) and Distinguished Adjunct Professor at King Abdulaziz University (Jeddah, Saudi Arabia). Additionally, he is deputy director of the Andalusian Research Institute in Data Science and Computational Intelligence (DaSCI) and heads the Knowledge Discovery and Intelligent Systems Research Laboratory. He has published more than ten books and over 300 articles in journals and scientific conferences. Currently, his work has received over 18,000 citations according to Google Scholar, including more than 2200 citations in 2020. In the last five years, he has published more than 60 papers in international journals indexed in the JCR (around 70% of them belonging to first quartile journals) and he has edited some Springer books “Supervised Descriptive Pattern Mining” (2018), “Multiple Instance Learning - Foundations and Algorithms” (2016), and “Pattern Mining with Evolutionary Algorithms” (2016). He has also been involved in more than 20 research projects supported by the Spanish and Andalusian governments and the European Union. He currently belongs to the editorial board of PeerJ Computer Science, Information Fusion and Engineering Applications of Artificial Intelligence journals, being also associate editor of Applied Computational Intelligence and Soft Computing and IEEE Transactions on Cybernetics. Finally, he is editor-in-chief of Progress in Artificial Intelligence. He is a Senior Member of the IEEE Computer, the IEEE Computational Intelligence, and the IEEE Systems, Man, and Cybernetics Societies, and the Association of Computing Machinery (ACM). Finally, his main research interests include data science, computational intelligence, and their applications.",institutionString:null,institution:{name:"University of Córdoba",institutionURL:null,country:{name:"Spain"}}},editorTwo:null,editorThree:null},{id:"26",title:"Machine Learning and Data Mining",coverUrl:"https://cdn.intechopen.com/series_topics/covers/26.jpg",isOpenForSubmission:!0,editor:{id:"24555",title:"Dr.",name:"Marco Antonio",middleName:null,surname:"Aceves Fernandez",slug:"marco-antonio-aceves-fernandez",fullName:"Marco Antonio Aceves Fernandez",profilePictureURL:"https://mts.intechopen.com/storage/users/24555/images/system/24555.jpg",biography:"Dr. Marco Antonio Aceves Fernandez obtained his B.Sc. (Eng.) in Telematics from the Universidad de Colima, Mexico. He obtained both his M.Sc. and Ph.D. from the University of Liverpool, England, in the field of Intelligent Systems. He is a full professor at the Universidad Autonoma de Queretaro, Mexico, and a member of the National System of Researchers (SNI) since 2009. Dr. Aceves Fernandez has published more than 80 research papers as well as a number of book chapters and congress papers. He has contributed in more than 20 funded research projects, both academic and industrial, in the area of artificial intelligence, ranging from environmental, biomedical, automotive, aviation, consumer, and robotics to other applications. He is also a honorary president at the National Association of Embedded Systems (AMESE), a senior member of the IEEE, and a board member of many institutions. His research interests include intelligent and embedded systems.",institutionString:"Universidad Autonoma de Queretaro",institution:{name:"Autonomous University of Queretaro",institutionURL:null,country:{name:"Mexico"}}},editorTwo:null,editorThree:null},{id:"27",title:"Multi-Agent Systems",coverUrl:"https://cdn.intechopen.com/series_topics/covers/27.jpg",isOpenForSubmission:!0,editor:{id:"148497",title:"Dr.",name:"Mehmet",middleName:"Emin",surname:"Aydin",slug:"mehmet-aydin",fullName:"Mehmet Aydin",profilePictureURL:"https://mts.intechopen.com/storage/users/148497/images/system/148497.jpg",biography:"Dr. Mehmet Emin Aydin is a Senior Lecturer with the Department of Computer Science and Creative Technology, the University of the West of England, Bristol, UK. His research interests include swarm intelligence, parallel and distributed metaheuristics, machine learning, intelligent agents and multi-agent systems, resource planning, scheduling and optimization, combinatorial optimization. Dr. Aydin is currently a Fellow of Higher Education Academy, UK, a member of EPSRC College, a senior member of IEEE and a senior member of ACM. In addition to being a member of advisory committees of many international conferences, he is an Editorial Board Member of various peer-reviewed international journals. He has served as guest editor for a number of special issues of peer-reviewed international journals.",institutionString:null,institution:{name:"University of the West of England",institutionURL:null,country:{name:"United Kingdom"}}},editorTwo:null,editorThree:null}]},overviewPageOFChapters:{paginationCount:6,paginationItems:[{id:"82526",title:"Deep Multiagent Reinforcement Learning Methods Addressing the Scalability Challenge",doi:"10.5772/intechopen.105627",signatures:"Theocharis Kravaris and George A. Vouros",slug:"deep-multiagent-reinforcement-learning-methods-addressing-the-scalability-challenge",totalDownloads:19,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Multi-Agent Technologies and Machine Learning",coverURL:"https://cdn.intechopen.com/books/images_new/11445.jpg",subseries:{id:"27",title:"Multi-Agent Systems"}}},{id:"82196",title:"Multi-Features Assisted Age Invariant Face Recognition and Retrieval Using CNN with Scale Invariant Heat Kernel Signature",doi:"10.5772/intechopen.104944",signatures:"Kamarajugadda Kishore Kumar and Movva Pavani",slug:"multi-features-assisted-age-invariant-face-recognition-and-retrieval-using-cnn-with-scale-invariant-",totalDownloads:14,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Pattern Recognition - New Insights",coverURL:"https://cdn.intechopen.com/books/images_new/11442.jpg",subseries:{id:"26",title:"Machine Learning and Data Mining"}}},{id:"82063",title:"Evaluating Similarities and Differences between Machine Learning and Traditional Statistical Modeling in Healthcare Analytics",doi:"10.5772/intechopen.105116",signatures:"Michele Bennett, Ewa J. Kleczyk, Karin Hayes and Rajesh Mehta",slug:"evaluating-similarities-and-differences-between-machine-learning-and-traditional-statistical-modelin",totalDownloads:7,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Machine Learning and Data Mining - Annual Volume 2022",coverURL:"https://cdn.intechopen.com/books/images_new/11422.jpg",subseries:{id:"26",title:"Machine Learning and Data Mining"}}},{id:"81791",title:"Self-Supervised Contrastive Representation Learning in Computer Vision",doi:"10.5772/intechopen.104785",signatures:"Yalin Bastanlar and Semih Orhan",slug:"self-supervised-contrastive-representation-learning-in-computer-vision",totalDownloads:59,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Pattern Recognition - New Insights",coverURL:"https://cdn.intechopen.com/books/images_new/11442.jpg",subseries:{id:"26",title:"Machine Learning and Data Mining"}}}]},overviewPagePublishedBooks:{paginationCount:11,paginationItems:[{type:"book",id:"7723",title:"Artificial Intelligence",subtitle:"Applications in Medicine and Biology",coverURL:"https://cdn.intechopen.com/books/images_new/7723.jpg",slug:"artificial-intelligence-applications-in-medicine-and-biology",publishedDate:"July 31st 2019",editedByType:"Edited by",bookSignature:"Marco Antonio Aceves-Fernandez",hash:"a3852659e727f95c98c740ed98146011",volumeInSeries:1,fullTitle:"Artificial Intelligence - Applications in Medicine and Biology",editors:[{id:"24555",title:"Dr.",name:"Marco Antonio",middleName:null,surname:"Aceves Fernandez",slug:"marco-antonio-aceves-fernandez",fullName:"Marco Antonio Aceves Fernandez",profilePictureURL:"https://mts.intechopen.com/storage/users/24555/images/system/24555.jpg",biography:"Dr. Marco Antonio Aceves Fernandez obtained his B.Sc. (Eng.) in Telematics from the Universidad de Colima, Mexico. He obtained both his M.Sc. and Ph.D. from the University of Liverpool, England, in the field of Intelligent Systems. He is a full professor at the Universidad Autonoma de Queretaro, Mexico, and a member of the National System of Researchers (SNI) since 2009. Dr. Aceves Fernandez has published more than 80 research papers as well as a number of book chapters and congress papers. He has contributed in more than 20 funded research projects, both academic and industrial, in the area of artificial intelligence, ranging from environmental, biomedical, automotive, aviation, consumer, and robotics to other applications. He is also a honorary president at the National Association of Embedded Systems (AMESE), a senior member of the IEEE, and a board member of many institutions. His research interests include intelligent and embedded systems.",institutionString:"Universidad Autonoma de Queretaro",institution:{name:"Autonomous University of Queretaro",institutionURL:null,country:{name:"Mexico"}}}]},{type:"book",id:"7726",title:"Swarm Intelligence",subtitle:"Recent Advances, New Perspectives and Applications",coverURL:"https://cdn.intechopen.com/books/images_new/7726.jpg",slug:"swarm-intelligence-recent-advances-new-perspectives-and-applications",publishedDate:"December 4th 2019",editedByType:"Edited by",bookSignature:"Javier Del Ser, Esther Villar and Eneko Osaba",hash:"e7ea7e74ce7a7a8e5359629e07c68d31",volumeInSeries:2,fullTitle:"Swarm Intelligence - Recent Advances, New Perspectives and Applications",editors:[{id:"49813",title:"Dr.",name:"Javier",middleName:null,surname:"Del Ser",slug:"javier-del-ser",fullName:"Javier Del Ser",profilePictureURL:"https://mts.intechopen.com/storage/users/49813/images/system/49813.png",biography:"Prof. Dr. Javier Del Ser received his first PhD in Telecommunication Engineering (Cum Laude) from the University of Navarra, Spain, in 2006, and a second PhD in Computational Intelligence (Summa Cum Laude) from the University of Alcala, Spain, in 2013. He is currently a principal researcher in data analytics and optimisation at TECNALIA (Spain), a visiting fellow at the Basque Center for Applied Mathematics (BCAM) and a part-time lecturer at the University of the Basque Country (UPV/EHU). His research interests gravitate on the use of descriptive, prescriptive and predictive algorithms for data mining and optimization in a diverse range of application fields such as Energy, Transport, Telecommunications, Health and Industry, among others. In these fields he has published more than 240 articles, co-supervised 8 Ph.D. theses, edited 6 books, coauthored 7 patents and participated/led more than 40 research projects. He is a Senior Member of the IEEE, and a recipient of the Biscay Talent prize for his academic career.",institutionString:"Tecnalia Research & Innovation",institution:{name:"Tecnalia",institutionURL:null,country:{name:"Spain"}}}]},{type:"book",id:"7656",title:"Fuzzy Logic",subtitle:null,coverURL:"https://cdn.intechopen.com/books/images_new/7656.jpg",slug:"fuzzy-logic",publishedDate:"February 5th 2020",editedByType:"Edited by",bookSignature:"Constantin Volosencu",hash:"54f092d4ffe0abf5e4172a80025019bc",volumeInSeries:3,fullTitle:"Fuzzy Logic",editors:[{id:"1063",title:"Prof.",name:"Constantin",middleName:null,surname:"Volosencu",slug:"constantin-volosencu",fullName:"Constantin Volosencu",profilePictureURL:"https://mts.intechopen.com/storage/users/1063/images/system/1063.png",biography:"Prof. Dr. Constantin Voloşencu graduated as an engineer from\nPolitehnica University of Timișoara, Romania, where he also\nobtained a doctorate degree. He is currently a full professor in\nthe Department of Automation and Applied Informatics at the\nsame university. Dr. Voloşencu is the author of ten books, seven\nbook chapters, and more than 160 papers published in journals\nand conference proceedings. He has also edited twelve books and\nhas twenty-seven patents to his name. He is a manager of research grants, editor in\nchief and member of international journal editorial boards, a former plenary speaker, a member of scientific committees, and chair at international conferences. His\nresearch is in the fields of control systems, control of electric drives, fuzzy control\nsystems, neural network applications, fault detection and diagnosis, sensor network\napplications, monitoring of distributed parameter systems, and power ultrasound\napplications. He has developed automation equipment for machine tools, spooling\nmachines, high-power ultrasound processes, and more.",institutionString:'"Politechnica" University Timişoara',institution:null}]},{type:"book",id:"9963",title:"Advances and Applications in Deep Learning",subtitle:null,coverURL:"https://cdn.intechopen.com/books/images_new/9963.jpg",slug:"advances-and-applications-in-deep-learning",publishedDate:"December 9th 2020",editedByType:"Edited by",bookSignature:"Marco Antonio Aceves-Fernandez",hash:"0d51ba46f22e55cb89140f60d86a071e",volumeInSeries:4,fullTitle:"Advances and Applications in Deep Learning",editors:[{id:"24555",title:"Dr.",name:"Marco Antonio",middleName:null,surname:"Aceves Fernandez",slug:"marco-antonio-aceves-fernandez",fullName:"Marco Antonio Aceves Fernandez",profilePictureURL:"https://mts.intechopen.com/storage/users/24555/images/system/24555.jpg",biography:"Dr. Marco Antonio Aceves Fernandez obtained his B.Sc. (Eng.) in Telematics from the Universidad de Colima, Mexico. He obtained both his M.Sc. and Ph.D. from the University of Liverpool, England, in the field of Intelligent Systems. He is a full professor at the Universidad Autonoma de Queretaro, Mexico, and a member of the National System of Researchers (SNI) since 2009. Dr. Aceves Fernandez has published more than 80 research papers as well as a number of book chapters and congress papers. He has contributed in more than 20 funded research projects, both academic and industrial, in the area of artificial intelligence, ranging from environmental, biomedical, automotive, aviation, consumer, and robotics to other applications. He is also a honorary president at the National Association of Embedded Systems (AMESE), a senior member of the IEEE, and a board member of many institutions. His research interests include intelligent and embedded systems.",institutionString:"Universidad Autonoma de Queretaro",institution:{name:"Autonomous University of Queretaro",institutionURL:null,country:{name:"Mexico"}}}]}]},openForSubmissionBooks:{paginationCount:1,paginationItems:[{id:"11478",title:"Recent Advances in the Study of Dyslexia",coverURL:"https://cdn.intechopen.com/books/images_new/11478.jpg",hash:"26764a18c6b776698823e0e1c3022d2f",secondStepPassed:!0,currentStepOfPublishingProcess:3,submissionDeadline:"June 30th 2022",isOpenForSubmission:!0,editors:[{id:"294281",title:"Prof.",name:"Jonathan",surname:"Glazzard",slug:"jonathan-glazzard",fullName:"Jonathan Glazzard"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null}]},onlineFirstChapters:{paginationCount:21,paginationItems:[{id:"83000",title:"Purine and Pyrimidine Pathways as Antimalarial Targets",doi:"10.5772/intechopen.106468",signatures:"Yacoba V.T. Minnow and Vern L. Schramm",slug:"purine-and-pyrimidine-pathways-as-antimalarial-targets",totalDownloads:1,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Malaria - Recent Advances, and New Perspectives",coverURL:"https://cdn.intechopen.com/books/images_new/11576.jpg",subseries:{id:"5",title:"Parasitic Infectious Diseases"}}},{id:"83065",title:"Interventions and Practical Approaches to Reduce the Burden of Malaria on School-Aged Children",doi:"10.5772/intechopen.106469",signatures:"Andrew Macnab",slug:"interventions-and-practical-approaches-to-reduce-the-burden-of-malaria-on-school-aged-children",totalDownloads:4,totalCrossrefCites:0,totalDimensionsCites:0,authors:[{name:"Andrew",surname:"Macnab"}],book:{title:"Malaria - Recent Advances, and New Perspectives",coverURL:"https://cdn.intechopen.com/books/images_new/11576.jpg",subseries:{id:"5",title:"Parasitic Infectious Diseases"}}},{id:"82804",title:"Psychiatric Problems in HIV Care",doi:"10.5772/intechopen.106077",signatures:"Seggane Musisi and Noeline Nakasujja",slug:"psychiatric-problems-in-hiv-care",totalDownloads:2,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Future Opportunities and Tools for Emerging Challenges for HIV/AIDS Control",coverURL:"https://cdn.intechopen.com/books/images_new/11575.jpg",subseries:{id:"6",title:"Viral Infectious Diseases"}}},{id:"82827",title:"Epidemiology and Control of Schistosomiasis",doi:"10.5772/intechopen.105170",signatures:"Célestin Kyambikwa Bisangamo",slug:"epidemiology-and-control-of-schistosomiasis",totalDownloads:6,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"New Horizons for Schistosomiasis Research",coverURL:"https://cdn.intechopen.com/books/images_new/10829.jpg",subseries:{id:"5",title:"Parasitic Infectious Diseases"}}},{id:"82817",title:"Perspective Chapter: Microfluidic Technologies for On-Site Detection and Quantification of Infectious Diseases - The Experience with SARS-CoV-2/COVID-19",doi:"10.5772/intechopen.105950",signatures:"Andres Escobar and Chang-qing Xu",slug:"perspective-chapter-microfluidic-technologies-for-on-site-detection-and-quantification-of-infectious",totalDownloads:3,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"SARS-CoV-2 Variants - Two Years After",coverURL:"https://cdn.intechopen.com/books/images_new/11573.jpg",subseries:{id:"6",title:"Viral Infectious Diseases"}}},{id:"82667",title:"Perspective Chapter: Analysis of SARS-CoV-2 Indirect Spreading Routes and Possible Countermeasures",doi:"10.5772/intechopen.105914",signatures:"Cesare Saccani, Marco Pellegrini and Alessandro Guzzini",slug:"perspective-chapter-analysis-of-sars-cov-2-indirect-spreading-routes-and-possible-countermeasures",totalDownloads:10,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"SARS-CoV-2 Variants - Two Years After",coverURL:"https://cdn.intechopen.com/books/images_new/11573.jpg",subseries:{id:"6",title:"Viral Infectious Diseases"}}},{id:"82559",title:"Perspective Chapter: Bioinformatics Study of the Evolution of SARS-CoV-2 Spike Protein",doi:"10.5772/intechopen.105915",signatures:"Črtomir Podlipnik, Radostina Alexandrova, Sebastian Pleško, Urban Bren and Marko Jukič",slug:"perspective-chapter-bioinformatics-study-of-the-evolution-of-sars-cov-2-spike-protein",totalDownloads:20,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"SARS-CoV-2 Variants - Two Years After",coverURL:"https://cdn.intechopen.com/books/images_new/11573.jpg",subseries:{id:"6",title:"Viral Infectious Diseases"}}},{id:"82521",title:"Challenges in Platelet Functions in HIV/AIDS Management",doi:"10.5772/intechopen.105731",signatures:"Gordon Ogweno",slug:"challenges-in-platelet-functions-in-hiv-aids-management",totalDownloads:16,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Future Opportunities and Tools for Emerging Challenges for HIV/AIDS Control",coverURL:"https://cdn.intechopen.com/books/images_new/11575.jpg",subseries:{id:"6",title:"Viral Infectious Diseases"}}},{id:"82701",title:"Pathology of Streptococcal Infections",doi:"10.5772/intechopen.105814",signatures:"Yutaka Tsutsumi",slug:"pathology-of-streptococcal-infections",totalDownloads:8,totalCrossrefCites:0,totalDimensionsCites:0,authors:[{name:"Yutaka",surname:"Tsutsumi"}],book:{title:"Streptococcal Infections",coverURL:"https://cdn.intechopen.com/books/images_new/10828.jpg",subseries:{id:"3",title:"Bacterial Infectious Diseases"}}},{id:"82634",title:"Bacterial Sexually Transmitted Disease",doi:"10.5772/intechopen.105747",signatures:"Lebeza Alemu Tenaw",slug:"bacterial-sexually-transmitted-disease",totalDownloads:12,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Bacterial Sexually Transmitted Infections - New Findings, Diagnosis, Treatment, and Prevention",coverURL:"https://cdn.intechopen.com/books/images_new/11569.jpg",subseries:{id:"3",title:"Bacterial Infectious Diseases"}}}]},subseriesFiltersForOFChapters:[{caption:"Fungal Infectious Diseases",value:4,count:1,group:"subseries"},{caption:"Parasitic Infectious Diseases",value:5,count:5,group:"subseries"},{caption:"Bacterial Infectious Diseases",value:3,count:5,group:"subseries"},{caption:"Viral Infectious Diseases",value:6,count:10,group:"subseries"}],publishedBooks:{paginationCount:1,paginationItems:[{type:"book",id:"10843",title:"Persistent Organic Pollutants (POPs)",subtitle:"Monitoring, Impact and Treatment",coverURL:"https://cdn.intechopen.com/books/images_new/10843.jpg",slug:"persistent-organic-pollutants-pops-monitoring-impact-and-treatment",publishedDate:"April 13th 2022",editedByType:"Edited by",bookSignature:"Mohamed Nageeb Rashed",hash:"f5b1589f0a990b6114fef2dadc735dd9",volumeInSeries:1,fullTitle:"Persistent Organic Pollutants (POPs) - Monitoring, Impact and Treatment",editors:[{id:"63465",title:"Prof.",name:"Mohamed Nageeb",middleName:null,surname:"Rashed",slug:"mohamed-nageeb-rashed",fullName:"Mohamed Nageeb Rashed",profilePictureURL:"https://mts.intechopen.com/storage/users/63465/images/system/63465.gif",institutionString:null,institution:{name:"Aswan University",institutionURL:null,country:{name:"Egypt"}}}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null}]},subseriesFiltersForPublishedBooks:[{group:"subseries",caption:"Pollution",value:38,count:1}],publicationYearFilters:[{group:"publicationYear",caption:"2022",value:2022,count:1}],authors:{paginationCount:755,paginationItems:[{id:"310674",title:"Dr.",name:"Pravin",middleName:null,surname:"Kendrekar",slug:"pravin-kendrekar",fullName:"Pravin Kendrekar",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/310674/images/system/310674.jpg",biography:"Dr. Pravin Kendrekar, MSc, MBA, Ph.D., is currently a visiting scientist at the Lipid Nanostructure Laboratory, University of Central Lancashire, England. He previously worked as a post-doctoral fellow at the Ben-Gurion University of Negev, Israel; University of the Free State, South Africa; and Central University of Technology Bloemfontein, South Africa. He obtained his Ph.D. in Organic Chemistry from Nagaoka University of Technology, Japan. He has published more than seventy-four journal articles and attended several national and international conferences as speaker and chair. Dr. Kendrekar has received many international awards. He has several funded projects, namely, anti-malaria drug development, MRSA, and SARS-CoV-2 activity of curcumin and its formulations. He has filed four patents in collaboration with the University of Central Lancashire and Mayo Clinic Infectious Diseases. His present research includes organic synthesis, drug discovery and development, biochemistry, nanoscience, and nanotechnology.",institutionString:"Visiting Scientist at Lipid Nanostructures Laboratory, Centre for Smart Materials, School of Natural Sciences, University of Central Lancashire",institution:null},{id:"428125",title:"Dr.",name:"Vinayak",middleName:null,surname:"Adimule",slug:"vinayak-adimule",fullName:"Vinayak Adimule",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/428125/images/system/428125.jpg",biography:"Dr. Vinayak Adimule, MSc, Ph.D., is a professor and dean of R&D, Angadi Institute of Technology and Management, India. He has 15 years of research experience as a senior research scientist and associate research scientist in R&D organizations. He has published more than fifty research articles as well as several book chapters. He has two Indian patents and two international patents to his credit. Dr. Adimule has attended, chaired, and presented papers at national and international conferences. He is a guest editor for Topics in Catalysis and other journals. He is also an editorial board member, life member, and associate member for many international societies and research institutions. His research interests include nanoelectronics, material chemistry, artificial intelligence, sensors and actuators, bio-nanomaterials, and medicinal chemistry.",institutionString:"Angadi Institute of Technology and Management",institution:null},{id:"284317",title:"Prof.",name:"Kantharaju",middleName:null,surname:"Kamanna",slug:"kantharaju-kamanna",fullName:"Kantharaju Kamanna",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/284317/images/21050_n.jpg",biography:"Prof. K. Kantharaju has received Bachelor of science (PCM), master of science (Organic Chemistry) and Doctor of Philosophy in Chemistry from Bangalore University. He worked as a Executive Research & Development @ Cadila Pharmaceuticals Ltd, Ahmedabad. He received DBT-postdoc fellow @ Molecular Biophysics Unit, Indian Institute of Science, Bangalore under the supervision of Prof. P. Balaram, later he moved to NIH-postdoc researcher at Drexel University College of Medicine, Philadelphia, USA, after his return from postdoc joined NITK-Surthakal as a Adhoc faculty at department of chemistry. Since from August 2013 working as a Associate Professor, and in 2016 promoted to Profeesor in the School of Basic Sciences: Department of Chemistry and having 20 years of teaching and research experiences.",institutionString:null,institution:{name:"Rani Channamma University, Belagavi",country:{name:"India"}}},{id:"158492",title:"Prof.",name:"Yusuf",middleName:null,surname:"Tutar",slug:"yusuf-tutar",fullName:"Yusuf Tutar",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/158492/images/system/158492.jpeg",biography:"Prof. Dr. Yusuf Tutar conducts his research at the Hamidiye Faculty of Pharmacy, Department of Basic Pharmaceutical Sciences, Division of Biochemistry, University of Health Sciences, Turkey. He is also a faculty member in the Molecular Oncology Program. He obtained his MSc and Ph.D. at Oregon State University and Texas Tech University, respectively. He pursued his postdoctoral studies at Rutgers University Medical School and the National Institutes of Health (NIH/NIDDK), USA. His research focuses on biochemistry, biophysics, genetics, molecular biology, and molecular medicine with specialization in the fields of drug design, protein structure-function, protein folding, prions, microRNA, pseudogenes, molecular cancer, epigenetics, metabolites, proteomics, genomics, protein expression, and characterization by spectroscopic and calorimetric methods.",institutionString:"University of Health Sciences",institution:null},{id:"180528",title:"Dr.",name:"Hiroyuki",middleName:null,surname:"Kagechika",slug:"hiroyuki-kagechika",fullName:"Hiroyuki Kagechika",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/180528/images/system/180528.jpg",biography:"Hiroyuki Kagechika received his bachelor’s degree and Ph.D. in Pharmaceutical Sciences from the University of Tokyo, Japan, where he served as an associate professor until 2004. He is currently a professor at the Institute of Biomaterials and Bioengineering (IBB), Tokyo Medical and Dental University (TMDU). From 2010 to 2012, he was the dean of the Graduate School of Biomedical Science. Since 2012, he has served as the vice dean of the Graduate School of Medical and Dental Sciences. He has been the director of the IBB since 2020. Dr. Kagechika’s major research interests are the medicinal chemistry of retinoids, vitamins D/K, and nuclear receptors. He has developed various compounds including a drug for acute promyelocytic leukemia.",institutionString:"Tokyo Medical and Dental University",institution:{name:"Tokyo Medical and Dental University",country:{name:"Japan"}}},{id:"94311",title:"Prof.",name:"Martins",middleName:"Ochubiojo",surname:"Ochubiojo Emeje",slug:"martins-ochubiojo-emeje",fullName:"Martins Ochubiojo Emeje",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/94311/images/system/94311.jpeg",biography:"Martins Emeje obtained a BPharm with distinction from Ahmadu Bello University, Nigeria, and an MPharm and Ph.D. from the University of Nigeria (UNN), where he received the best Ph.D. award and was enlisted as UNN’s “Face of Research.” He established the first nanomedicine center in Nigeria and was the pioneer head of the intellectual property and technology transfer as well as the technology innovation and support center. Prof. Emeje’s several international fellowships include the prestigious Raman fellowship. He has published more than 150 articles and patents. He is also the head of R&D at NIPRD and holds a visiting professor position at Nnamdi Azikiwe University, Nigeria. He has a postgraduate certificate in Project Management from Walden University, Minnesota, as well as a professional teaching certificate and a World Bank certification in Public Procurement. Prof. Emeje was a national chairman of academic pharmacists in Nigeria and the 2021 winner of the May & Baker Nigeria Plc–sponsored prize for professional service in research and innovation.",institutionString:"National Institute for Pharmaceutical Research and Development",institution:{name:"National Institute for Pharmaceutical Research and Development",country:{name:"Nigeria"}}},{id:"436430",title:"Associate Prof.",name:"Mesut",middleName:null,surname:"Işık",slug:"mesut-isik",fullName:"Mesut Işık",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/436430/images/19686_n.jpg",biography:null,institutionString:null,institution:{name:"Bilecik University",country:{name:"Turkey"}}},{id:"268659",title:"Ms.",name:"Xianquan",middleName:null,surname:"Zhan",slug:"xianquan-zhan",fullName:"Xianquan Zhan",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/268659/images/8143_n.jpg",biography:"Dr. Zhan received his undergraduate and graduate training in the fields of preventive medicine and epidemiology and statistics at the West China University of Medical Sciences in China during 1989 to 1999. He received his post-doctoral training in oncology and cancer proteomics for two years at the Cancer Research Institute of Human Medical University in China. In 2001, he went to the University of Tennessee Health Science Center (UTHSC) in USA, where he was a post-doctoral researcher and focused on mass spectrometry and cancer proteomics. Then, he was appointed as an Assistant Professor of Neurology, UTHSC in 2005. He moved to the Cleveland Clinic in USA as a Project Scientist/Staff in 2006 where he focused on the studies of eye disease proteomics and biomarkers. He returned to UTHSC as an Assistant Professor of Neurology in the end of 2007, engaging in proteomics and biomarker studies of lung diseases and brain tumors, and initiating the studies of predictive, preventive, and personalized medicine (PPPM) in cancer. In 2010, he was promoted to Associate Professor of Neurology, UTHSC. Currently, he is a Professor at Xiangya Hospital of Central South University in China, Fellow of Royal Society of Medicine (FRSM), the European EPMA National Representative in China, Regular Member of American Association for the Advancement of Science (AAAS), European Cooperation of Science and Technology (e-COST) grant evaluator, Associate Editors of BMC Genomics, BMC Medical Genomics, EPMA Journal, and Frontiers in Endocrinology, Executive Editor-in-Chief of Med One. He has\npublished 116 peer-reviewed research articles, 16 book chapters, 2 books, and 2 US patents. His current main research interest focuses on the studies of cancer proteomics and biomarkers, and the use of modern omics techniques and systems biology for PPPM in cancer, and on the development and use of 2DE-LC/MS for the large-scale study of human proteoforms.",institutionString:null,institution:{name:"Xiangya Hospital Central South University",country:{name:"China"}}},{id:"40482",title:null,name:"Rizwan",middleName:null,surname:"Ahmad",slug:"rizwan-ahmad",fullName:"Rizwan Ahmad",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/40482/images/system/40482.jpeg",biography:"Dr. Rizwan Ahmad is a University Professor and Coordinator, Quality and Development, College of Medicine, Imam Abdulrahman bin Faisal University, Saudi Arabia. Previously, he was Associate Professor of Human Function, Oman Medical College, Oman, and SBS University, Dehradun. Dr. Ahmad completed his education at Aligarh Muslim University, Aligarh. He has published several articles in peer-reviewed journals, chapters, and edited books. His area of specialization is free radical biochemistry and autoimmune diseases.",institutionString:"Imam Abdulrahman Bin Faisal University",institution:{name:"Imam Abdulrahman Bin Faisal University",country:{name:"Saudi Arabia"}}},{id:"41865",title:"Prof.",name:"Farid A.",middleName:null,surname:"Badria",slug:"farid-a.-badria",fullName:"Farid A. Badria",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/41865/images/system/41865.jpg",biography:"Farid A. Badria, Ph.D., is the recipient of several awards, including The World Academy of Sciences (TWAS) Prize for Public Understanding of Science; the World Intellectual Property Organization (WIPO) Gold Medal for best invention; Outstanding Arab Scholar, Kuwait; and the Khwarizmi International Award, Iran. He has 250 publications, 12 books, 20 patents, and several marketed pharmaceutical products to his credit. He continues to lead research projects on developing new therapies for liver, skin disorders, and cancer. Dr. Badria was listed among the world’s top 2% of scientists in medicinal and biomolecular chemistry in 2019 and 2020. He is a member of the Arab Development Fund, Kuwait; International Cell Research Organization–United Nations Educational, Scientific and Cultural Organization (ICRO–UNESCO), Chile; and UNESCO Biotechnology France",institutionString:"Mansoura University",institution:{name:"Mansoura University",country:{name:"Egypt"}}},{id:"329385",title:"Dr.",name:"Rajesh K.",middleName:"Kumar",surname:"Singh",slug:"rajesh-k.-singh",fullName:"Rajesh K. Singh",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/329385/images/system/329385.png",biography:"Dr. Singh received a BPharm (2003) and MPharm (2005) from Panjab University, Chandigarh, India, and a Ph.D. (2013) from Punjab Technical University (PTU), Jalandhar, India. He has more than sixteen years of teaching experience and has supervised numerous postgraduate and Ph.D. students. He has to his credit more than seventy papers in SCI- and SCOPUS-indexed journals, fifty-five conference proceedings, four books, six Best Paper Awards, and five projects from different government agencies. He is currently an editorial board member of eight international journals and a reviewer for more than fifty scientific journals. He received Top Reviewer and Excellent Peer Reviewer Awards from Publons in 2016 and 2017, respectively. He is also on the panel of The International Reviewer for reviewing research proposals for grants from the Royal Society. He also serves as a Publons Academy mentor and Bentham brand ambassador.",institutionString:"Punjab Technical University",institution:{name:"Punjab Technical University",country:{name:"India"}}},{id:"142388",title:"Dr.",name:"Thiago",middleName:"Gomes",surname:"Gomes Heck",slug:"thiago-gomes-heck",fullName:"Thiago Gomes Heck",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/142388/images/7259_n.jpg",biography:null,institutionString:null,institution:{name:"Universidade Regional do Noroeste do Estado do Rio Grande do Sul",country:{name:"Brazil"}}},{id:"336273",title:"Assistant Prof.",name:"Janja",middleName:null,surname:"Zupan",slug:"janja-zupan",fullName:"Janja Zupan",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/336273/images/14853_n.jpeg",biography:"Janja Zupan graduated in 2005 at the Department of Clinical Biochemistry (superviser prof. dr. Janja Marc) in the field of genetics of osteoporosis. Since November 2009 she is working as a Teaching Assistant at the Faculty of Pharmacy, Department of Clinical Biochemistry. In 2011 she completed part of her research and PhD work at Institute of Genetics and Molecular Medicine, University of Edinburgh. She finished her PhD entitled The influence of the proinflammatory cytokines on the RANK/RANKL/OPG in bone tissue of osteoporotic and osteoarthritic patients in 2012. From 2014-2016 she worked at the Institute of Biomedical Sciences, University of Aberdeen as a postdoctoral research fellow on UK Arthritis research project where she gained knowledge in mesenchymal stem cells and regenerative medicine. She returned back to University of Ljubljana, Faculty of Pharmacy in 2016. She is currently leading project entitled Mesenchymal stem cells-the keepers of tissue endogenous regenerative capacity facing up to aging of the musculoskeletal system funded by Slovenian Research Agency.",institutionString:null,institution:{name:"University of Ljubljana",country:{name:"Slovenia"}}},{id:"357453",title:"Dr.",name:"Radheshyam",middleName:null,surname:"Maurya",slug:"radheshyam-maurya",fullName:"Radheshyam Maurya",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/357453/images/16535_n.jpg",biography:null,institutionString:null,institution:{name:"University of Hyderabad",country:{name:"India"}}},{id:"418340",title:"Dr.",name:"Jyotirmoi",middleName:null,surname:"Aich",slug:"jyotirmoi-aich",fullName:"Jyotirmoi Aich",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y000038Ugi5QAC/Profile_Picture_2022-04-15T07:48:28.png",biography:"Biotechnologist with 15 years of research including 6 years of teaching experience. Demonstrated record of scientific achievements through consistent publication record (H index = 13, with 874 citations) in high impact journals such as Nature Communications, Oncotarget, Annals of Oncology, PNAS, and AJRCCM, etc. Strong research professional with a post-doctorate from ACTREC where I gained experimental oncology experience in clinical settings and a doctorate from IGIB where I gained expertise in asthma pathophysiology. A well-trained biotechnologist with diverse experience on the bench across different research themes ranging from asthma to cancer and other infectious diseases. An individual with a strong commitment and innovative mindset. Have the ability to work on diverse projects such as regenerative and molecular medicine with an overall mindset of improving healthcare.",institutionString:"DY Patil Deemed to Be University",institution:null},{id:"349288",title:"Prof.",name:"Soumya",middleName:null,surname:"Basu",slug:"soumya-basu",fullName:"Soumya Basu",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y000035QxIDQA0/Profile_Picture_2022-04-15T07:47:01.jpg",biography:"Soumya Basu, Ph.D., is currently working as an Associate Professor at Dr. D. Y. Patil Biotechnology and Bioinformatics Institute, Dr. D. Y. Patil Vidyapeeth, Pune, Maharashtra, India. With 16+ years of trans-disciplinary research experience in Drug Design, development, and pre-clinical validation; 20+ research article publications in journals of repute, 9+ years of teaching experience, trained with cross-disciplinary education, Dr. Basu is a life-long learner and always thrives for new challenges.\r\nHer research area is the design and synthesis of small molecule partial agonists of PPAR-γ in lung cancer. She is also using artificial intelligence and deep learning methods to understand the exosomal miRNA’s role in cancer metastasis. Dr. Basu is the recipient of many awards including the Early Career Research Award from the Department of Science and Technology, Govt. of India. She is a reviewer of many journals like Molecular Biology Reports, Frontiers in Oncology, RSC Advances, PLOS ONE, Journal of Biomolecular Structure & Dynamics, Journal of Molecular Graphics and Modelling, etc. She has edited and authored/co-authored 21 journal papers, 3 book chapters, and 15 abstracts. She is a Board of Studies member at her university. She is a life member of 'The Cytometry Society”-in India and 'All India Cell Biology Society”- in India.",institutionString:"Dr. D.Y. Patil Vidyapeeth, Pune",institution:{name:"Dr. D.Y. Patil Vidyapeeth, Pune",country:{name:"India"}}},{id:"354817",title:"Dr.",name:"Anubhab",middleName:null,surname:"Mukherjee",slug:"anubhab-mukherjee",fullName:"Anubhab Mukherjee",position:null,profilePictureURL:"https://intech-files.s3.amazonaws.com/0033Y0000365PbRQAU/ProfilePicture%202022-04-15%2005%3A11%3A18.480",biography:"A former member of Laboratory of Nanomedicine, Brigham and Women’s Hospital, Harvard University, Boston, USA, Dr. Anubhab Mukherjee is an ardent votary of science who strives to make an impact in the lives of those afflicted with cancer and other chronic/acute ailments. He completed his Ph.D. from CSIR-Indian Institute of Chemical Technology, Hyderabad, India, having been skilled with RNAi, liposomal drug delivery, preclinical cell and animal studies. He pursued post-doctoral research at College of Pharmacy, Health Science Center, Texas A & M University and was involved in another postdoctoral research at Department of Translational Neurosciences and Neurotherapeutics, John Wayne Cancer Institute, Santa Monica, California. In 2015, he worked in Harvard-MIT Health Sciences & Technology as a visiting scientist. He has substantial experience in nanotechnology-based formulation development and successfully served various Indian organizations to develop pharmaceuticals and nutraceutical products. He is an inventor in many US patents and an author in many peer-reviewed articles, book chapters and books published in various media of international repute. Dr. Mukherjee is currently serving as Principal Scientist, R&D at Esperer Onco Nutrition (EON) Pvt. Ltd. and heads the Hyderabad R&D center of the organization.",institutionString:"Esperer Onco Nutrition Pvt Ltd.",institution:null},{id:"319365",title:"Assistant Prof.",name:"Manash K.",middleName:null,surname:"Paul",slug:"manash-k.-paul",fullName:"Manash K. Paul",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/319365/images/system/319365.png",biography:"Manash K. Paul is a scientist and Principal Investigator at the University of California Los Angeles. He has contributed significantly to the fields of stem cell biology, regenerative medicine, and lung cancer. His research focuses on various signaling processes involved in maintaining stem cell homeostasis during the injury-repair process, deciphering the lung stem cell niche, pulmonary disease modeling, immuno-oncology, and drug discovery. He is currently investigating the role of extracellular vesicles in premalignant lung cell migration and detecting the metastatic phenotype of lung cancer via artificial intelligence-based analyses of exosomal Raman signatures. Dr. Paul also works on spatial multiplex immunofluorescence-based tissue mapping to understand the immune repertoire in lung cancer. Dr. Paul has published in more than sixty-five peer-reviewed international journals and is highly cited. He is the recipient of many awards, including the UCLA Vice Chancellor’s award and the 2022 AAISCR-R Vijayalaxmi Award for Innovative Cancer Research. He is a senior member of the Institute of Electrical and Electronics Engineers (IEEE) and an editorial board member for several international journals.",institutionString:"University of California Los Angeles",institution:{name:"University of California Los Angeles",country:{name:"United States of America"}}},{id:"311457",title:"Dr.",name:"Júlia",middleName:null,surname:"Scherer Santos",slug:"julia-scherer-santos",fullName:"Júlia Scherer Santos",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/311457/images/system/311457.jpg",biography:"Dr. Júlia Scherer Santos works in the areas of cosmetology, nanotechnology, pharmaceutical technology, beauty, and aesthetics. Dr. Santos also has experience as a professor of graduate courses. Graduated in Pharmacy, specialization in Cosmetology and Cosmeceuticals applied to aesthetics, specialization in Aesthetic and Cosmetic Health, and a doctorate in Pharmaceutical Nanotechnology. Teaching experience in Pharmacy and Aesthetics and Cosmetics courses. She works mainly on the following subjects: nanotechnology, cosmetology, pharmaceutical technology, aesthetics.",institutionString:"Universidade Federal de Juiz de Fora",institution:{name:"Universidade Federal de Juiz de Fora",country:{name:"Brazil"}}},{id:"219081",title:"Dr.",name:"Abdulsamed",middleName:null,surname:"Kükürt",slug:"abdulsamed-kukurt",fullName:"Abdulsamed Kükürt",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/219081/images/system/219081.png",biography:"Dr. Kükürt graduated from Uludağ University in Turkey. He started his academic career as a Research Assistant in the Department of Biochemistry at Kafkas University. In 2019, he completed his Ph.D. program in the Department of Biochemistry at the Institute of Health Sciences. He is currently working at the Department of Biochemistry, Kafkas University. He has 27 published research articles in academic journals, 11 book chapters, and 37 papers. He took part in 10 academic projects. He served as a reviewer for many articles. He still serves as a member of the review board in many academic journals. He is currently working on the protective activity of phenolic compounds in disorders associated with oxidative stress and inflammation.",institutionString:null,institution:{name:"Kafkas University",country:{name:"Turkey"}}},{id:"178366",title:"Dr.",name:"Volkan",middleName:null,surname:"Gelen",slug:"volkan-gelen",fullName:"Volkan Gelen",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/178366/images/system/178366.jpg",biography:"Volkan Gelen is a Physiology specialist who received his veterinary degree from Kafkas University in 2011. Between 2011-2015, he worked as an assistant at Atatürk University, Faculty of Veterinary Medicine, Department of Physiology. In 2016, he joined Kafkas University, Faculty of Veterinary Medicine, Department of Physiology as an assistant professor. Dr. Gelen has been engaged in various academic activities at Kafkas University since 2016. There he completed 5 projects and has 3 ongoing projects. He has 60 articles published in scientific journals and 20 poster presentations in scientific congresses. His research interests include physiology, endocrine system, cancer, diabetes, cardiovascular system diseases, and isolated organ bath system studies.",institutionString:"Kafkas University",institution:{name:"Kafkas University",country:{name:"Turkey"}}},{id:"418963",title:"Dr.",name:"Augustine Ododo",middleName:"Augustine",surname:"Osagie",slug:"augustine-ododo-osagie",fullName:"Augustine Ododo Osagie",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/418963/images/16900_n.jpg",biography:"Born into the family of Osagie, a prince of the Benin Kingdom. I am currently an academic in the Department of Medical Biochemistry, University of Benin. Part of the duties are to teach undergraduate students and conduct academic research.",institutionString:null,institution:{name:"University of Benin",country:{name:"Nigeria"}}},{id:"192992",title:"Prof.",name:"Shagufta",middleName:null,surname:"Perveen",slug:"shagufta-perveen",fullName:"Shagufta Perveen",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/192992/images/system/192992.png",biography:"Prof. Shagufta Perveen is a Distinguish Professor in the Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia. Dr. Perveen has acted as the principal investigator of major research projects funded by the research unit of King Saud University. She has more than ninety original research papers in peer-reviewed journals of international repute to her credit. She is a fellow member of the Royal Society of Chemistry UK and the American Chemical Society of the United States.",institutionString:"King Saud University",institution:{name:"King Saud University",country:{name:"Saudi Arabia"}}},{id:"49848",title:"Dr.",name:"Wen-Long",middleName:null,surname:"Hu",slug:"wen-long-hu",fullName:"Wen-Long Hu",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/49848/images/system/49848.jpg",biography:"Wen-Long Hu is Chief of the Division of Acupuncture, Department of Chinese Medicine at Kaohsiung Chang Gung Memorial Hospital, as well as an adjunct associate professor at Fooyin University and Kaohsiung Medical University. Wen-Long is President of Taiwan Traditional Chinese Medicine Medical Association. He has 28 years of experience in clinical practice in laser acupuncture therapy and 34 years in acupuncture. He is an invited speaker for lectures and workshops in laser acupuncture at many symposiums held by medical associations. He owns the patent for herbal preparation and producing, and for the supercritical fluid-treated needle. Dr. Hu has published three books, 12 book chapters, and more than 30 papers in reputed journals, besides serving as an editorial board member of repute.",institutionString:"Kaohsiung Chang Gung Memorial Hospital",institution:{name:"Kaohsiung Chang Gung Memorial Hospital",country:{name:"Taiwan"}}},{id:"298472",title:"Prof.",name:"Andrey V.",middleName:null,surname:"Grechko",slug:"andrey-v.-grechko",fullName:"Andrey V. Grechko",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/298472/images/system/298472.png",biography:"Andrey Vyacheslavovich Grechko, Ph.D., Professor, is a Corresponding Member of the Russian Academy of Sciences. He graduated from the Semashko Moscow Medical Institute (Semashko National Research Institute of Public Health) with a degree in Medicine (1998), the Clinical Department of Dermatovenerology (2000), and received a second higher education in Psychology (2009). Professor A.V. Grechko held the position of Сhief Physician of the Central Clinical Hospital in Moscow. He worked as a professor at the faculty and was engaged in scientific research at the Medical University. Starting in 2013, he has been the initiator of the creation of the Federal Scientific and Clinical Center for Intensive Care and Rehabilitology, Moscow, Russian Federation, where he also serves as Director since 2015. He has many years of experience in research and teaching in various fields of medicine, is an author/co-author of more than 200 scientific publications, 13 patents, 15 medical books/chapters, including Chapter in Book «Metabolomics», IntechOpen, 2020 «Metabolomic Discovery of Microbiota Dysfunction as the Cause of Pathology».",institutionString:"Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology",institution:null},{id:"199461",title:"Prof.",name:"Natalia V.",middleName:null,surname:"Beloborodova",slug:"natalia-v.-beloborodova",fullName:"Natalia V. Beloborodova",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/199461/images/system/199461.jpg",biography:'Natalia Vladimirovna Beloborodova was educated at the Pirogov Russian National Research Medical University, with a degree in pediatrics in 1980, a Ph.D. in 1987, and a specialization in Clinical Microbiology from First Moscow State Medical University in 2004. She has been a Professor since 1996. Currently, she is the Head of the Laboratory of Metabolism, a division of the Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russian Federation. N.V. Beloborodova has many years of clinical experience in the field of intensive care and surgery. She studies infectious complications and sepsis. She initiated a series of interdisciplinary clinical and experimental studies based on the concept of integrating human metabolism and its microbiota. Her scientific achievements are widely known: she is the recipient of the Marie E. Coates Award \\"Best lecturer-scientist\\" Gustafsson Fund, Karolinska Institutes, Stockholm, Sweden, and the International Sepsis Forum Award, Pasteur Institute, Paris, France (2014), etc. Professor N.V. Beloborodova wrote 210 papers, five books, 10 chapters and has edited four books.',institutionString:"Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology",institution:null},{id:"354260",title:"Ph.D.",name:"Tércio Elyan",middleName:"Azevedo",surname:"Azevedo Martins",slug:"tercio-elyan-azevedo-martins",fullName:"Tércio Elyan Azevedo Martins",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/354260/images/16241_n.jpg",biography:"Graduated in Pharmacy from the Federal University of Ceará with the modality in Industrial Pharmacy, Specialist in Production and Control of Medicines from the University of São Paulo (USP), Master in Pharmaceuticals and Medicines from the University of São Paulo (USP) and Doctor of Science in the program of Pharmaceuticals and Medicines by the University of São Paulo. Professor at Universidade Paulista (UNIP) in the areas of chemistry, cosmetology and trichology. Assistant Coordinator of the Higher Course in Aesthetic and Cosmetic Technology at Universidade Paulista Campus Chácara Santo Antônio. Experience in the Pharmacy area, with emphasis on Pharmacotechnics, Pharmaceutical Technology, Research and Development of Cosmetics, acting mainly on topics such as cosmetology, antioxidant activity, aesthetics, photoprotection, cyclodextrin and thermal analysis.",institutionString:null,institution:{name:"University of Sao Paulo",country:{name:"Brazil"}}},{id:"334285",title:"Ph.D. Student",name:"Sameer",middleName:"Kumar",surname:"Jagirdar",slug:"sameer-jagirdar",fullName:"Sameer Jagirdar",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/334285/images/14691_n.jpg",biography:"I\\'m a graduate student at the center for biosystems science and engineering at the Indian Institute of Science, Bangalore, India. I am interested in studying host-pathogen interactions at the biomaterial interface.",institutionString:null,institution:{name:"Indian Institute of Science Bangalore",country:{name:"India"}}},{id:"329248",title:"Dr.",name:"Md. Faheem",middleName:null,surname:"Haider",slug:"md.-faheem-haider",fullName:"Md. Faheem Haider",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/329248/images/system/329248.jpg",biography:"Dr. Md. Faheem Haider completed his BPharm in 2012 at Integral University, Lucknow, India. In 2014, he completed his MPharm with specialization in Pharmaceutics at Babasaheb Bhimrao Ambedkar University, Lucknow, India. He received his Ph.D. degree from Jamia Hamdard University, New Delhi, India, in 2018. He was selected for the GPAT six times and his best All India Rank was 34. Currently, he is an assistant professor at Integral University. Previously he was an assistant professor at IIMT University, Meerut, India. He has experience teaching DPharm, Pharm.D, BPharm, and MPharm students. He has more than five publications in reputed journals to his credit. Dr. Faheem’s research area is the development and characterization of nanoformulation for the delivery of drugs to various organs.",institutionString:"Integral University",institution:{name:"Integral University",country:{name:"India"}}},{id:"329795",title:"Dr.",name:"Mohd Aftab",middleName:"Aftab",surname:"Siddiqui",slug:"mohd-aftab-siddiqui",fullName:"Mohd Aftab Siddiqui",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/329795/images/system/329795.png",biography:"Dr. Mohd Aftab Siddiqui is an assistant professor in the Faculty of Pharmacy, Integral University, Lucknow, India, where he obtained a Ph.D. in Pharmacology in 2020. He also obtained a BPharm and MPharm from the same university in 2013 and 2015, respectively. His area of research is the pharmacological screening of herbal drugs/natural products in liver cancer and cardiac diseases. He is a member of many professional bodies and has guided many MPharm and PharmD research projects. Dr. Siddiqui has many national and international publications and one German patent to his credit.",institutionString:"Integral University",institution:null}]}},subseries:{item:{id:"8",type:"subseries",title:"Bioinspired Technology and Biomechanics",keywords:"Bioinspired Systems, Biomechanics, Assistive Technology, Rehabilitation",scope:'Bioinspired technologies take advantage of understanding the actual biological system to provide solutions to problems in several areas. Recently, bioinspired systems have been successfully employing biomechanics to develop and improve assistive technology and rehabilitation devices. The research topic "Bioinspired Technology and Biomechanics" welcomes studies reporting recent advances in bioinspired technologies that contribute to individuals\' health, inclusion, and rehabilitation. Possible contributions can address (but are not limited to) the following research topics: Bioinspired design and control of exoskeletons, orthoses, and prostheses; Experimental evaluation of the effect of assistive devices (e.g., influence on gait, balance, and neuromuscular system); Bioinspired technologies for rehabilitation, including clinical studies reporting evaluations; Application of neuromuscular and biomechanical models to the development of bioinspired technology.',coverUrl:"https://cdn.intechopen.com/series_topics/covers/8.jpg",hasOnlineFirst:!0,hasPublishedBooks:!0,annualVolume:11404,editor:{id:"144937",title:"Prof.",name:"Adriano",middleName:"De Oliveira",surname:"Andrade",slug:"adriano-andrade",fullName:"Adriano Andrade",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRC8QQAW/Profile_Picture_1625219101815",biography:"Dr. Adriano de Oliveira Andrade graduated in Electrical Engineering at the Federal University of Goiás (Brazil) in 1997. He received his MSc and PhD in Biomedical Engineering respectively from the Federal University of Uberlândia (UFU, Brazil) in 2000 and from the University of Reading (UK) in 2005. He completed a one-year Post-Doctoral Fellowship awarded by the DFAIT (Foreign Affairs and International Trade Canada) at the Institute of Biomedical Engineering of the University of New Brunswick (Canada) in 2010. Currently, he is Professor in the Faculty of Electrical Engineering (UFU). He has authored and co-authored more than 200 peer-reviewed publications in Biomedical Engineering. He has been a researcher of The National Council for Scientific and Technological Development (CNPq-Brazil) since 2009. He has served as an ad-hoc consultant for CNPq, CAPES (Coordination for the Improvement of Higher Education Personnel), FINEP (Brazilian Innovation Agency), and other funding bodies on several occasions. He was the Secretary of the Brazilian Society of Biomedical Engineering (SBEB) from 2015 to 2016, President of SBEB (2017-2018) and Vice-President of SBEB (2019-2020). He was the head of the undergraduate program in Biomedical Engineering of the Federal University of Uberlândia (2015 - June/2019) and the head of the Centre for Innovation and Technology Assessment in Health (NIATS/UFU) since 2010. He is the head of the Postgraduate Program in Biomedical Engineering (UFU, July/2019 - to date). He was the secretary of the Parkinson's Disease Association of Uberlândia (2018-2019). Dr. Andrade's primary area of research is focused towards getting information from the neuromuscular system to understand its strategies of organization, adaptation and controlling in the context of motor neuron diseases. His research interests include Biomedical Signal Processing and Modelling, Assistive Technology, Rehabilitation Engineering, Neuroengineering and Parkinson's Disease.",institutionString:null,institution:{name:"Federal University of Uberlândia",institutionURL:null,country:{name:"Brazil"}}},editorTwo:null,editorThree:null,series:{id:"7",title:"Biomedical Engineering",doi:"10.5772/intechopen.71985",issn:"2631-5343"},editorialBoard:[{id:"49517",title:"Prof.",name:"Hitoshi",middleName:null,surname:"Tsunashima",slug:"hitoshi-tsunashima",fullName:"Hitoshi Tsunashima",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002aYTP4QAO/Profile_Picture_1625819726528",institutionString:null,institution:{name:"Nihon University",institutionURL:null,country:{name:"Japan"}}},{id:"425354",title:"Dr.",name:"Marcus",middleName:"Fraga",surname:"Vieira",slug:"marcus-vieira",fullName:"Marcus Vieira",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y00003BJSgIQAX/Profile_Picture_1627904687309",institutionString:null,institution:{name:"Universidade Federal de Goiás",institutionURL:null,country:{name:"Brazil"}}},{id:"196746",title:"Dr.",name:"Ramana",middleName:null,surname:"Vinjamuri",slug:"ramana-vinjamuri",fullName:"Ramana Vinjamuri",profilePictureURL:"https://mts.intechopen.com/storage/users/196746/images/system/196746.jpeg",institutionString:"University of Maryland, Baltimore County",institution:{name:"University of Maryland, Baltimore County",institutionURL:null,country:{name:"United States of America"}}}]},onlineFirstChapters:{paginationCount:18,paginationItems:[{id:"83115",title:"Fungi and Oomycetes–Allies in Eliminating Environmental Pathogens",doi:"10.5772/intechopen.106498",signatures:"Iasmina Luca",slug:"fungi-and-oomycetes-allies-in-eliminating-environmental-pathogens",totalDownloads:0,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Animal Welfare - New Insights",coverURL:"https://cdn.intechopen.com/books/images_new/11579.jpg",subseries:{id:"19",title:"Animal Science"}}},{id:"82991",title:"Diseases of the Canine Prostate Gland",doi:"10.5772/intechopen.105835",signatures:"Sabine Schäfer-Somi",slug:"diseases-of-the-canine-prostate-gland",totalDownloads:8,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Recent Advances in Canine Medicine",coverURL:"https://cdn.intechopen.com/books/images_new/11580.jpg",subseries:{id:"19",title:"Animal Science"}}},{id:"82773",title:"Canine Transmissible Venereal Tumor: An Infectious Neoplasia in Dogs",doi:"10.5772/intechopen.106150",signatures:"Chanokchon Setthawongsin, Somporn Techangamsuwan and Anudep Rungsipipat",slug:"canine-transmissible-venereal-tumor-an-infectious-neoplasia-in-dogs",totalDownloads:17,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Recent Advances in Canine Medicine",coverURL:"https://cdn.intechopen.com/books/images_new/11580.jpg",subseries:{id:"19",title:"Animal Science"}}},{id:"82797",title:"Anatomical Guide to the Paranasal Sinuses of Domestic Animals",doi:"10.5772/intechopen.106157",signatures:"Mohamed A.M. Alsafy, Samir A.A. El-Gendy and Catrin Sian Rutland",slug:"anatomical-guide-to-the-paranasal-sinuses-of-domestic-animals",totalDownloads:8,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Updates on Veterinary Anatomy and Physiology",coverURL:"https://cdn.intechopen.com/books/images_new/10665.jpg",subseries:{id:"19",title:"Animal Science"}}},{id:"82457",title:"Canine Hearing Management",doi:"10.5772/intechopen.105515",signatures:"Peter M. Skip Scheifele, Devan Marshall, Stephen Lee, Paul Reid, Thomas McCreery and David Byrne",slug:"canine-hearing-management",totalDownloads:9,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Recent Advances in Canine Medicine",coverURL:"https://cdn.intechopen.com/books/images_new/11580.jpg",subseries:{id:"19",title:"Animal Science"}}},{id:"82285",title:"Parvovirus Vectors: The Future of Gene Therapy",doi:"10.5772/intechopen.105085",signatures:"Megha Gupta",slug:"parvovirus-vectors-the-future-of-gene-therapy",totalDownloads:16,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Recent Advances in Canine Medicine",coverURL:"https://cdn.intechopen.com/books/images_new/11580.jpg",subseries:{id:"19",title:"Animal Science"}}},{id:"82170",title:"Equine Stress: Neuroendocrine Physiology and Pathophysiology",doi:"10.5772/intechopen.105045",signatures:"Milomir Kovac, Tatiana Vladimirovna Ippolitova, Sergey Pozyabin, Ruslan Aliev, Viktoria Lobanova, Nevena Drakul and Catrin S. Rutland",slug:"equine-stress-neuroendocrine-physiology-and-pathophysiology",totalDownloads:33,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Updates on Veterinary Anatomy and Physiology",coverURL:"https://cdn.intechopen.com/books/images_new/10665.jpg",subseries:{id:"19",title:"Animal Science"}}},{id:"81793",title:"Canine parvovirus-2: An Emerging Threat to Young Pets",doi:"10.5772/intechopen.104846",signatures:"Mithilesh Singh, Rajendran Manikandan, Ujjwal Kumar De, Vishal Chander, Babul Rudra Paul, Saravanan Ramakrishnan and Darshini Maramreddy",slug:"canine-parvovirus-2-an-emerging-threat-to-young-pets",totalDownloads:34,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Recent Advances in Canine Medicine",coverURL:"https://cdn.intechopen.com/books/images_new/11580.jpg",subseries:{id:"19",title:"Animal Science"}}},{id:"81271",title:"The Diversity of Parvovirus Telomeres",doi:"10.5772/intechopen.102684",signatures:"Marianne Laugel, Emilie Lecomte, Eduard Ayuso, Oumeya Adjali, Mathieu Mével and Magalie Penaud-Budloo",slug:"the-diversity-of-parvovirus-telomeres",totalDownloads:43,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Recent Advances in Canine Medicine",coverURL:"https://cdn.intechopen.com/books/images_new/11580.jpg",subseries:{id:"19",title:"Animal Science"}}},{id:"79209",title:"Virtual Physiology: A Tool for the 21st Century",doi:"10.5772/intechopen.99671",signatures:"Carmen Nóbrega, Maria Aires Pereira, Catarina Coelho, Isabel Brás, Ana Cristina Mega, Carla Santos, Fernando Esteves, Rita Cruz, Ana I. Faustino-Rocha, Paula A. Oliveira, João Mesquita and Helena Vala",slug:"virtual-physiology-a-tool-for-the-21st-century",totalDownloads:165,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Updates on Veterinary Anatomy and Physiology",coverURL:"https://cdn.intechopen.com/books/images_new/10665.jpg",subseries:{id:"19",title:"Animal Science"}}},{id:"78543",title:"Pulmonary Vein: Embryology, Anatomy, Function and Disease",doi:"10.5772/intechopen.100051",signatures:"Chan I-Ping and Hsueh Tung",slug:"pulmonary-vein-embryology-anatomy-function-and-disease",totalDownloads:196,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Updates on Veterinary Anatomy and Physiology",coverURL:"https://cdn.intechopen.com/books/images_new/10665.jpg",subseries:{id:"19",title:"Animal Science"}}},{id:"78564",title:"Anatomy of the Rhesus Monkey (Macaca mulatta): The Essentials for the Biomedical Researcher",doi:"10.5772/intechopen.99067",signatures:"Christophe Casteleyn and Jaco Bakker",slug:"anatomy-of-the-rhesus-monkey-macaca-mulatta-the-essentials-for-the-biomedical-researcher",totalDownloads:413,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Updates on Veterinary Anatomy and Physiology",coverURL:"https://cdn.intechopen.com/books/images_new/10665.jpg",subseries:{id:"19",title:"Animal Science"}}},{id:"77999",title:"Bronchus-Associated Lymphoid Tissue (BALT) Histology and Its Role in Various Pathologies",doi:"10.5772/intechopen.99366",signatures:"Tuba Parlak Ak",slug:"bronchus-associated-lymphoid-tissue-balt-histology-and-its-role-in-various-pathologies",totalDownloads:231,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Updates on Veterinary Anatomy and Physiology",coverURL:"https://cdn.intechopen.com/books/images_new/10665.jpg",subseries:{id:"19",title:"Animal Science"}}},{id:"78242",title:"Genomic Instability and Cyto-Genotoxic Damage in Animal Species",doi:"10.5772/intechopen.99685",signatures:"María Evarista Arellano-García, Olivia Torres-Bugarín, Maritza Roxana García-García, Daniel García-Flores, Yanis Toledano-Magaña, Cinthya Sofia Sanabria-Mora, Sandra Castro-Gamboa and Juan Carlos García-Ramos",slug:"genomic-instability-and-cyto-genotoxic-damage-in-animal-species",totalDownloads:159,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Updates on Veterinary Anatomy and Physiology",coverURL:"https://cdn.intechopen.com/books/images_new/10665.jpg",subseries:{id:"19",title:"Animal Science"}}},{id:"78503",title:"Biomechanics of the Canine Elbow Joint",doi:"10.5772/intechopen.99569",signatures:"Thomas Rohwedder",slug:"biomechanics-of-the-canine-elbow-joint",totalDownloads:212,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Updates on Veterinary Anatomy and Physiology",coverURL:"https://cdn.intechopen.com/books/images_new/10665.jpg",subseries:{id:"19",title:"Animal Science"}}},{id:"78018",title:"Application of Noble Metals in the Advances in Animal Disease Diagnostics",doi:"10.5772/intechopen.99162",signatures:"Gabriel Alexis S.P. Tubalinal, Leonard Paulo G. Lucero, Jim Andreus V. Mangahas, Marvin A. Villanueva and Claro N. Mingala",slug:"application-of-noble-metals-in-the-advances-in-animal-disease-diagnostics",totalDownloads:118,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Updates on Veterinary Anatomy and Physiology",coverURL:"https://cdn.intechopen.com/books/images_new/10665.jpg",subseries:{id:"19",title:"Animal Science"}}}]},publishedBooks:{paginationCount:5,paginationItems:[{type:"book",id:"8737",title:"Rabies Virus at the Beginning of 21st Century",subtitle:null,coverURL:"https://cdn.intechopen.com/books/images_new/8737.jpg",slug:"rabies-virus-at-the-beginning-of-21st-century",publishedDate:"May 11th 2022",editedByType:"Edited by",bookSignature:"Sergey Tkachev",hash:"49cce3f548da548c718c865feb343509",volumeInSeries:9,fullTitle:"Rabies Virus at the Beginning of 21st Century",editors:[{id:"61139",title:"Dr.",name:"Sergey",middleName:null,surname:"Tkachev",slug:"sergey-tkachev",fullName:"Sergey Tkachev",profilePictureURL:"https://mts.intechopen.com/storage/users/61139/images/system/61139.png",institutionString:"Russian Academy of Sciences",institution:{name:"Russian Academy of Sciences",institutionURL:null,country:{name:"Russia"}}}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null},{type:"book",id:"10497",title:"Canine Genetics, Health and Medicine",subtitle:null,coverURL:"https://cdn.intechopen.com/books/images_new/10497.jpg",slug:"canine-genetics-health-and-medicine",publishedDate:"June 2nd 2021",editedByType:"Edited by",bookSignature:"Catrin Rutland",hash:"b91512e31ce34032e560362e6cbccc1c",volumeInSeries:7,fullTitle:"Canine Genetics, Health and Medicine",editors:[{id:"202192",title:"Dr.",name:"Catrin",middleName:null,surname:"Rutland",slug:"catrin-rutland",fullName:"Catrin Rutland",profilePictureURL:"https://mts.intechopen.com/storage/users/202192/images/system/202192.png",institutionString:null,institution:{name:"University of Nottingham",institutionURL:null,country:{name:"United Kingdom"}}}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null},{type:"book",id:"9081",title:"Equine Science",subtitle:null,coverURL:"https://cdn.intechopen.com/books/images_new/9081.jpg",slug:"equine-science",publishedDate:"September 23rd 2020",editedByType:"Edited by",bookSignature:"Catrin Rutland and Albert Rizvanov",hash:"ac415ef2f5450fa80fdb9cf6cf32cd2d",volumeInSeries:5,fullTitle:"Equine Science",editors:[{id:"202192",title:"Dr.",name:"Catrin",middleName:null,surname:"Rutland",slug:"catrin-rutland",fullName:"Catrin Rutland",profilePictureURL:"https://mts.intechopen.com/storage/users/202192/images/system/202192.png",institutionString:null,institution:{name:"University of Nottingham",institutionURL:null,country:{name:"United Kingdom"}}}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null},{type:"book",id:"8524",title:"Lactation in Farm Animals",subtitle:"Biology, Physiological Basis, Nutritional Requirements, and Modelization",coverURL:"https://cdn.intechopen.com/books/images_new/8524.jpg",slug:"lactation-in-farm-animals-biology-physiological-basis-nutritional-requirements-and-modelization",publishedDate:"January 22nd 2020",editedByType:"Edited by",bookSignature:"Naceur M'Hamdi",hash:"2aa2a9a0ec13040bbf0455e34625504e",volumeInSeries:3,fullTitle:"Lactation in Farm Animals - Biology, Physiological Basis, Nutritional Requirements, and Modelization",editors:[{id:"73376",title:"Dr.",name:"Naceur",middleName:null,surname:"M'Hamdi",slug:"naceur-m'hamdi",fullName:"Naceur M'Hamdi",profilePictureURL:"https://mts.intechopen.com/storage/users/73376/images/system/73376.jpg",institutionString:null,institution:null}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null},{type:"book",id:"7144",title:"Veterinary Anatomy and Physiology",subtitle:null,coverURL:"https://cdn.intechopen.com/books/images_new/7144.jpg",slug:"veterinary-anatomy-and-physiology",publishedDate:"March 13th 2019",editedByType:"Edited by",bookSignature:"Catrin Sian Rutland and Valentina Kubale",hash:"75cdacb570e0e6d15a5f6e69640d87c9",volumeInSeries:2,fullTitle:"Veterinary Anatomy and Physiology",editors:[{id:"202192",title:"Dr.",name:"Catrin",middleName:null,surname:"Rutland",slug:"catrin-rutland",fullName:"Catrin Rutland",profilePictureURL:"https://mts.intechopen.com/storage/users/202192/images/system/202192.png",institutionString:null,institution:{name:"University of Nottingham",institutionURL:null,country:{name:"United Kingdom"}}}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null}]},testimonialsList:[{id:"8",text:"I work with IntechOpen for a number of reasons: their professionalism, their mission in support of Open Access publishing, and the quality of their peer-reviewed publications, but also because they believe in equality.",author:{id:"202192",name:"Catrin",surname:"Rutland",institutionString:null,profilePictureURL:"https://mts.intechopen.com/storage/users/202192/images/system/202192.png",slug:"catrin-rutland",institution:{id:"134",name:"University of Nottingham",country:{id:null,name:"United Kingdom"}}}},{id:"18",text:"It was great publishing with IntechOpen, the process was straightforward and I had support all along.",author:{id:"71579",name:"Berend",surname:"Olivier",institutionString:"Utrecht University",profilePictureURL:"https://mts.intechopen.com/storage/users/71579/images/system/71579.png",slug:"berend-olivier",institution:{id:"253",name:"Utrecht University",country:{id:null,name:"Netherlands"}}}},{id:"27",text:"The opportunity to work with a prestigious publisher allows for the possibility to collaborate with more research groups interested in animal nutrition, leading to the development of new feeding strategies and food valuation while being more sustainable with the environment, allowing more readers to learn about the subject.",author:{id:"175967",name:"Manuel",surname:"Gonzalez Ronquillo",institutionString:null,profilePictureURL:"https://mts.intechopen.com/storage/users/175967/images/system/175967.png",slug:"manuel-gonzalez-ronquillo",institution:{id:"6221",name:"Universidad Autónoma del Estado de México",country:{id:null,name:"Mexico"}}}}]},submityourwork:{pteSeriesList:[{id:"14",title:"Artificial Intelligence",numberOfPublishedBooks:11,numberOfPublishedChapters:91,numberOfOpenTopics:6,numberOfUpcomingTopics:0,issn:"2633-1403",doi:"10.5772/intechopen.79920",isOpenForSubmission:!0},{id:"7",title:"Biomedical Engineering",numberOfPublishedBooks:12,numberOfPublishedChapters:108,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2631-5343",doi:"10.5772/intechopen.71985",isOpenForSubmission:!0}],lsSeriesList:[{id:"11",title:"Biochemistry",numberOfPublishedBooks:33,numberOfPublishedChapters:333,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2632-0983",doi:"10.5772/intechopen.72877",isOpenForSubmission:!0},{id:"25",title:"Environmental Sciences",numberOfPublishedBooks:1,numberOfPublishedChapters:19,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2754-6713",doi:"10.5772/intechopen.100362",isOpenForSubmission:!0},{id:"10",title:"Physiology",numberOfPublishedBooks:14,numberOfPublishedChapters:145,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2631-8261",doi:"10.5772/intechopen.72796",isOpenForSubmission:!0}],hsSeriesList:[{id:"3",title:"Dentistry",numberOfPublishedBooks:11,numberOfPublishedChapters:144,numberOfOpenTopics:2,numberOfUpcomingTopics:0,issn:"2631-6218",doi:"10.5772/intechopen.71199",isOpenForSubmission:!0},{id:"6",title:"Infectious Diseases",numberOfPublishedBooks:13,numberOfPublishedChapters:124,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2631-6188",doi:"10.5772/intechopen.71852",isOpenForSubmission:!0},{id:"13",title:"Veterinary Medicine and Science",numberOfPublishedBooks:11,numberOfPublishedChapters:113,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2632-0517",doi:"10.5772/intechopen.73681",isOpenForSubmission:!0}],sshSeriesList:[{id:"22",title:"Business, Management and Economics",numberOfPublishedBooks:1,numberOfPublishedChapters:23,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2753-894X",doi:"10.5772/intechopen.100359",isOpenForSubmission:!0},{id:"23",title:"Education and Human Development",numberOfPublishedBooks:0,numberOfPublishedChapters:12,numberOfOpenTopics:1,numberOfUpcomingTopics:1,issn:null,doi:"10.5772/intechopen.100360",isOpenForSubmission:!0},{id:"24",title:"Sustainable Development",numberOfPublishedBooks:1,numberOfPublishedChapters:19,numberOfOpenTopics:5,numberOfUpcomingTopics:0,issn:"2753-6580",doi:"10.5772/intechopen.100361",isOpenForSubmission:!0}],subseriesList:[{id:"3",title:"Bacterial Infectious Diseases",scope:"