Descriptive statistics.
\\n\\n
IntechOpen was founded by scientists, for scientists, in order to make book publishing accessible around the globe. Over the last two decades, this has driven Open Access (OA) book publishing whilst levelling the playing field for global academics. Through our innovative publishing model and the support of the research community, we have now published over 5,700 Open Access books and are visited online by over three million academics every month. These researchers are increasingly working in broad technology-based subjects, driving multidisciplinary academic endeavours into human health, environment, and technology.
\\n\\nBy listening to our community, and in order to serve these rapidly growing areas which lie at the core of IntechOpen's expertise, we are launching a portfolio of Open Science journals:
\\n\\nAll three journals will publish under an Open Access model and embrace Open Science policies to help support the changing needs of academics in these fast-moving research areas. There will be direct links to preprint servers and data repositories, allowing full reproducibility and rapid dissemination of published papers to help accelerate the pace of research. Each journal has renowned Editors in Chief who will work alongside a global Editorial Board, delivering robust single-blind peer review. Supported by our internal editorial teams, this will ensure our authors will receive a quick, user-friendly, and personalised publishing experience.
\\n\\n"By launching our journals portfolio we are introducing new, dedicated homes for interdisciplinary technology-focused researchers to publish their work, whilst embracing Open Science and creating a unique global home for academics to disseminate their work. We are taking a leap toward Open Science continuing and expanding our fundamental commitment to openly sharing scientific research across the world, making it available for the benefit of all." Dr. Sara Uhac, IntechOpen CEO
\\n\\n"Our aim is to promote and create better science for a better world by increasing access to information and the latest scientific developments to all scientists, innovators, entrepreneurs and students and give them the opportunity to learn, observe and contribute to knowledge creation. Open Science promotes a swifter path from research to innovation to produce new products and services." Alex Lazinica, IntechOpen founder
\\n\\nIn conclusion, Natalia Reinic Babic, Head of Journal Publishing and Open Science at IntechOpen adds:
\\n\\n“On behalf of the journal team I’d like to thank all our Editors in Chief, Editorial Boards, internal supporting teams, and our scientific community for their continuous support in making this portfolio a reality - we couldn’t have done it without you! With your support in place, we are confident these journals will become as impactful and successful as our book publishing program and bring us closer to a more open (science) future.”
\\n\\nWe invite you to visit the journals homepage and learn more about the journal’s Editorial Boards, scope and vision as all three journals are now open for submissions.
\\n\\nFeel free to share this news on social media and help us mark this memorable moment!
\\n\\n\\n"}]',published:!0,mainMedia:{caption:"",originalUrl:"/media/original/237"}},components:[{type:"htmlEditorComponent",content:'
After years of being acknowledged as the world's leading publisher of Open Access books, today, we are proud to announce we’ve successfully launched a portfolio of Open Science journals covering rapidly expanding areas of interdisciplinary research.
\n\n\n\nIntechOpen was founded by scientists, for scientists, in order to make book publishing accessible around the globe. Over the last two decades, this has driven Open Access (OA) book publishing whilst levelling the playing field for global academics. Through our innovative publishing model and the support of the research community, we have now published over 5,700 Open Access books and are visited online by over three million academics every month. These researchers are increasingly working in broad technology-based subjects, driving multidisciplinary academic endeavours into human health, environment, and technology.
\n\nBy listening to our community, and in order to serve these rapidly growing areas which lie at the core of IntechOpen's expertise, we are launching a portfolio of Open Science journals:
\n\nAll three journals will publish under an Open Access model and embrace Open Science policies to help support the changing needs of academics in these fast-moving research areas. There will be direct links to preprint servers and data repositories, allowing full reproducibility and rapid dissemination of published papers to help accelerate the pace of research. Each journal has renowned Editors in Chief who will work alongside a global Editorial Board, delivering robust single-blind peer review. Supported by our internal editorial teams, this will ensure our authors will receive a quick, user-friendly, and personalised publishing experience.
\n\n"By launching our journals portfolio we are introducing new, dedicated homes for interdisciplinary technology-focused researchers to publish their work, whilst embracing Open Science and creating a unique global home for academics to disseminate their work. We are taking a leap toward Open Science continuing and expanding our fundamental commitment to openly sharing scientific research across the world, making it available for the benefit of all." Dr. Sara Uhac, IntechOpen CEO
\n\n"Our aim is to promote and create better science for a better world by increasing access to information and the latest scientific developments to all scientists, innovators, entrepreneurs and students and give them the opportunity to learn, observe and contribute to knowledge creation. Open Science promotes a swifter path from research to innovation to produce new products and services." Alex Lazinica, IntechOpen founder
\n\nIn conclusion, Natalia Reinic Babic, Head of Journal Publishing and Open Science at IntechOpen adds:
\n\n“On behalf of the journal team I’d like to thank all our Editors in Chief, Editorial Boards, internal supporting teams, and our scientific community for their continuous support in making this portfolio a reality - we couldn’t have done it without you! With your support in place, we are confident these journals will become as impactful and successful as our book publishing program and bring us closer to a more open (science) future.”
\n\nWe invite you to visit the journals homepage and learn more about the journal’s Editorial Boards, scope and vision as all three journals are now open for submissions.
\n\nFeel free to share this news on social media and help us mark this memorable moment!
\n\n\n'}],latestNews:[{slug:"webinar-introduction-to-open-science-wednesday-18-may-1-pm-cest-20220518",title:"Webinar: Introduction to Open Science | Wednesday 18 May, 1 PM CEST"},{slug:"step-in-the-right-direction-intechopen-launches-a-portfolio-of-open-science-journals-20220414",title:"Step in the Right Direction: IntechOpen Launches a Portfolio of Open Science Journals"},{slug:"let-s-meet-at-london-book-fair-5-7-april-2022-olympia-london-20220321",title:"Let’s meet at London Book Fair, 5-7 April 2022, Olympia London"},{slug:"50-books-published-as-part-of-intechopen-and-knowledge-unlatched-ku-collaboration-20220316",title:"50 Books published as part of IntechOpen and Knowledge Unlatched (KU) Collaboration"},{slug:"intechopen-joins-the-united-nations-sustainable-development-goals-publishers-compact-20221702",title:"IntechOpen joins the United Nations Sustainable Development Goals Publishers Compact"},{slug:"intechopen-signs-exclusive-representation-agreement-with-lsr-libros-servicios-y-representaciones-s-a-de-c-v-20211123",title:"IntechOpen Signs Exclusive Representation Agreement with LSR Libros Servicios y Representaciones S.A. de C.V"},{slug:"intechopen-expands-partnership-with-research4life-20211110",title:"IntechOpen Expands Partnership with Research4Life"},{slug:"introducing-intechopen-book-series-a-new-publishing-format-for-oa-books-20210915",title:"Introducing IntechOpen Book Series - A New Publishing Format for OA Books"}]},book:{item:{type:"book",id:"5450",leadTitle:null,fullTitle:"Polymerase Chain Reaction for Biomedical Applications",title:"Polymerase Chain Reaction for Biomedical Applications",subtitle:null,reviewType:"peer-reviewed",abstract:"Do you want to know the details that should be taken into consideration in order to have accurate conventional and real-time PCR results? If so, this book is for you. Polymerase Chain Reaction for Biomedical Applications is a collection of chapters for both novice and experienced scientists and technologists aiming to address obtaining an optimized real-time PCR result, simultaneous processing of a large number of samples and assays, performing PCR and RT-PCR on cell lysate without extraction of DNA or RNA, detecting false-positive PCR results, detecting organisms in viral and microbial diseases and hospital environment, following safety assessments of food products, and using PCR for introduction of mutations. This is a must-have book for any PCR laboratory.",isbn:"978-953-51-2796-3",printIsbn:"978-953-51-2795-6",pdfIsbn:"978-953-51-4133-4",doi:"10.5772/62968",price:119,priceEur:129,priceUsd:155,slug:"polymerase-chain-reaction-for-biomedical-applications",numberOfPages:184,isOpenForSubmission:!1,isInWos:1,isInBkci:!0,hash:"fcc7a13834f2748241be560e9c512a76",bookSignature:"Ali Samadikuchaksaraei",publishedDate:"December 14th 2016",coverURL:"https://cdn.intechopen.com/books/images_new/5450.jpg",numberOfDownloads:25961,numberOfWosCitations:41,numberOfCrossrefCitations:27,numberOfCrossrefCitationsByBook:0,numberOfDimensionsCitations:61,numberOfDimensionsCitationsByBook:0,hasAltmetrics:1,numberOfTotalCitations:129,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"April 12th 2016",dateEndSecondStepPublish:"May 3rd 2016",dateEndThirdStepPublish:"August 7th 2016",dateEndFourthStepPublish:"November 5th 2016",dateEndFifthStepPublish:"December 5th 2016",currentStepOfPublishingProcess:5,indexedIn:"1,2,3,4,5,6,8,9",editedByType:"Edited by",kuFlag:!1,featuredMarkup:null,editors:[{id:"187501",title:"Prof.",name:"Ali",middleName:null,surname:"Samadikuchaksaraei",slug:"ali-samadikuchaksaraei",fullName:"Ali Samadikuchaksaraei",profilePictureURL:"https://mts.intechopen.com/storage/users/187501/images/system/187501.jpeg",biography:"Professor Samadikuchaksaraei has received his MD degree from Iran University of Medical Sciences in 1998 and his Ph.D. degree from Imperial College London in 2005. His expertise is mainly focused on regenerative niche engineering for skeletal, integumentary and respiratory systems using stem cells and biomimetic materials. His publications, including book chapters, editorials, abstracts and original articles are published in collaboration with more than 300 scientists from more than 60 institutes in 10 different countries around the world. Some of his research outputs have been patented for commercial purposes. Prof. Samadikuchaksaraei serves on the editorial boards of several journals and regularly reviews for many high-profile publishers. Additionally, he reviews for granting bodies and patent and intellectual properties state organizations.",institutionString:"Iran University of Medical Sciences",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"0",totalChapterViews:"0",totalEditedBooks:"2",institution:{name:"Iran University of Medical Sciences",institutionURL:null,country:{name:"Iran"}}}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,coeditorOne:null,coeditorTwo:null,coeditorThree:null,coeditorFour:null,coeditorFive:null,topics:[{id:"384",title:"Chemical Biology",slug:"chemical-biology"}],chapters:[{id:"53176",title:"Guidelines for Successful Quantitative Gene Expression in Real- Time qPCR Assays",doi:"10.5772/65850",slug:"guidelines-for-successful-quantitative-gene-expression-in-real-time-qpcr-assays",totalDownloads:3555,totalCrossrefCites:3,totalDimensionsCites:4,hasAltmetrics:0,abstract:"This chapter was developed to provide some important guidelines for studies with quantitative PCR (qPCR) using either dyes or probes, citing several essential components necessary for a good PCR assay. The efficiency and specificity of quantitative PCR (qPCR) depend on several parameters related to mRNA quantification that must be controlled to avoid mistakes in data interpretation. Avoiding contamination with proteins, carbohydrate and phenolic compounds during RNA extraction and purification processes will improve RNA quality and provide reliable results. Specific primers and sensible probes are also crucial to intensify efficiency, specificity and fluorescence. Other parameters such as the optimization of primer concentrations and efficiency primer curves must be done. During gene-expression profile quantification, qPCR assays using reference genes are required to normalize the target gene expression data. These reference genes are checked for stability to identify the most stable genes among a group of candidate genes that will be used to normalize the qPCR data, using programs such as geNorm, BestKeeper and NormFinder. Additionally, the choice of appropriate reference genes for a specific experimental condition is fundamental. The main aim of this chapter is to provide guidelines and highlight precautions to obtain a successful qPCR assays.",signatures:"Antônio José Rocha, Rafael de Souza Miranda, Antônio Juscelino\nSudário Sousa and André Luis Coelho da Silva",downloadPdfUrl:"/chapter/pdf-download/53176",previewPdfUrl:"/chapter/pdf-preview/53176",authors:[{id:"188806",title:"Dr.",name:"Antônio",surname:"Rocha",slug:"antonio-rocha",fullName:"Antônio Rocha"}],corrections:null},{id:"52927",title:"High-Throughput Platforms in Real-Time PCR and Applications",doi:"10.5772/65760",slug:"high-throughput-platforms-in-real-time-pcr-and-applications",totalDownloads:2266,totalCrossrefCites:8,totalDimensionsCites:19,hasAltmetrics:0,abstract:"The miniaturization of reactions by designing nanoliter-scale PCR platforms, as Taqman® OpenArray®, Dynamic Array™, or SmartChip, has been a big step forward in real-time PCR. Each platform has some particular characteristics that differentiate them. These nanoliter-scale PCR platforms enable substantial savings in the amount of reagents and sample because the reaction volumes are at nanoliter levels. In addition, it is possible to perform thousands of reactions in a few hours. Therefore, high-throughput real-time PCR platforms result in promising systems that are capable of processing a large number of samples simultaneously and also to perform a large number of assays per sample. All of this can be translated in the amazing applicability of this technology in all kinds of analytical fields, such as medical research, animal science, and food safety, among others.",signatures:"Alexandre Lamas, Carlos Manuel Franco, Patricia Regal, José\nManuel Miranda, Beatriz Vázquez and Alberto Cepeda",downloadPdfUrl:"/chapter/pdf-download/52927",previewPdfUrl:"/chapter/pdf-preview/52927",authors:[{id:"127648",title:"Ms.",name:"Patricia",surname:"Regal",slug:"patricia-regal",fullName:"Patricia Regal"},{id:"171990",title:"Dr.",name:"José Manuel",surname:"Miranda",slug:"jose-manuel-miranda",fullName:"José Manuel Miranda"},{id:"189907",title:"Dr.",name:"Carlos",surname:"Franco",slug:"carlos-franco",fullName:"Carlos Franco"},{id:"194841",title:"Dr.",name:"Alexandre",surname:"Lamas",slug:"alexandre-lamas",fullName:"Alexandre Lamas"},{id:"194842",title:"Dr.",name:"Beatriz",surname:"Vázquez",slug:"beatriz-vazquez",fullName:"Beatriz Vázquez"},{id:"194843",title:"Dr.",name:"Aberto",surname:"Cepeda",slug:"aberto-cepeda",fullName:"Aberto Cepeda"}],corrections:null},{id:"52692",title:"Hot Cell-Direct PCR Aimed at Specific Cell Detection",doi:"10.5772/65806",slug:"hot-cell-direct-pcr-aimed-at-specific-cell-detection",totalDownloads:1584,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:1,abstract:"Since the polymerase chain reaction (PCR) was proposed, it has become an essential method in the field of biological gene analysis, providing a method to amplify DNA sequences of interest. To detect and/or analyze genes in cells, the gene or expressed gene must first be extracted before PCR. This procedure takes time and may result in the loss of samples. In order to avoid such drawbacks, two methods, hot cell-direct PCR and reverse transcription-PCR (RT-PCR), were invented, to detect genes in cells. Using hot cell-direct PCR, specific genes in microbial cells such as invA in Salmonella enterica have been easily detected and applied to discriminate Archaea from bacteria. As hot cell-direct PCR and RT-PCR are fairly simple processes, they can be applied to detect genes in single cells. We developed an original compact disc (CD)-shaped microfluidic device with microchambers for single-cell isolation and a detection system for expressed genes in isolated single cells in a microchamber on the device. We succeeded in the detection of PCR and RT-PCR products in individual cells and successfully detected S. enterica cells by hot cell-direct PCR. Expressed genes in Jurkat cells—human leukemia T cells—were analyzed by this method.",signatures:"Izumi Kubo, Yuko H. Itoh and Shunsuke Furutani",downloadPdfUrl:"/chapter/pdf-download/52692",previewPdfUrl:"/chapter/pdf-preview/52692",authors:[{id:"190351",title:"Prof.",name:"Izumi",surname:"Kubo",slug:"izumi-kubo",fullName:"Izumi Kubo"}],corrections:null},{id:"52932",title:"Regulatory Concern of Polymerase Chain Reaction (PCR) Carryover Contamination",doi:"10.5772/66294",slug:"regulatory-concern-of-polymerase-chain-reaction-pcr-carryover-contamination",totalDownloads:3271,totalCrossrefCites:4,totalDimensionsCites:13,hasAltmetrics:1,abstract:"Currently, DNA amplification techniques have become important detection tools. However, the extreme sensitivity of such techniques can easily result in contamination. This is a major problem in using these techniques routinely in a regulatory agency such as the Food and Drug Administration (FDA) because false-positive polymerase chain reaction (PCR) results will fail our mission. Preventing PCR carryover contamination and a capacity to rapidly determine false PCR positives are crucial. In the past, several methods have been used to prevent amplicon carryover contamination. In this chapter, we provide practical suggestions for PCR carryover contamination detection and prevention that work well with most PCR applications in our laboratory.",signatures:"Yuan Hu",downloadPdfUrl:"/chapter/pdf-download/52932",previewPdfUrl:"/chapter/pdf-preview/52932",authors:[{id:"189420",title:"Dr.",name:"Yuan",surname:"Hu",slug:"yuan-hu",fullName:"Yuan Hu"}],corrections:null},{id:"52768",title:"Multiplex Polymerase Chain Reaction Assay for Early Diagnosis of Viral Infection",doi:"10.5772/65771",slug:"multiplex-polymerase-chain-reaction-assay-for-early-diagnosis-of-viral-infection",totalDownloads:1929,totalCrossrefCites:2,totalDimensionsCites:5,hasAltmetrics:0,abstract:"Viral reactivation is one of the most serious complications for immunocompromised patients. Under immunosuppressive conditions, some viruses can be reactivated solely or simultaneously and may thus cause life-threatening infection. Therefore, the prompt and proper diagnosis of viral reactivation is important for the initiation of preemptive therapy. For this purpose, we recently developed a multiplex-virus polymerase chain reaction (PCR) assay. The multiplex PCR assay is designed to qualitatively measure the genomic DNA of 12 viruses at once: cytomegalovirus (CMV), human herpesvirus type 6 (HHV-6), HHV-7, HHV-8, Epstein-Barr virus (EBV), varicella-zoster virus (VZV), BK virus (BKV), JC virus (JCV), parvovirus B19 (ParvoB19), herpes simplex virus type 1 (HSV-1), HSV-2, and hepatitis B virus (HBV). When a specific PCR signal is obtained, the viral load is determined by a quantitative real-time PCR. The qualitative multiplex and quantitative real-time PCR procedures take only 3 hours to complete. With this assay system, we can identify viremia at the early stage and thereby prevent it from progressing to overt and symptomatic viral infection in immunocompromised patients, such as those receiving hematopoietic stem cell transplantation.",signatures:"Hiroko Tsunemine, Yuriko Yoshioka, Miho Nagao, Yasuhiro\nTomaru, Toshiharu Saitoh, Souichi Adachi, Norio Shimizu and\nTakayuki Takahashi",downloadPdfUrl:"/chapter/pdf-download/52768",previewPdfUrl:"/chapter/pdf-preview/52768",authors:[{id:"175658",title:"Dr.",name:"Hiroko",surname:"Tsunemine",slug:"hiroko-tsunemine",fullName:"Hiroko Tsunemine"}],corrections:null},{id:"52716",title:"The Advantages of Using Multiplex PCR for the Simultaneous Detection of Six Sexually Transmitted Diseases",doi:"10.5772/65901",slug:"the-advantages-of-using-multiplex-pcr-for-the-simultaneous-detection-of-six-sexually-transmitted-dis",totalDownloads:2447,totalCrossrefCites:2,totalDimensionsCites:2,hasAltmetrics:1,abstract:"Sexually transmitted diseases (STDs) are among the most common infections. Their clinical identification is difficult because STDs are often asymptomatic. Untreated infections with these pathogens can in time lead to serious consequences. It is documented that isolation of some of these bacteria from cultures is very difficult. Because there is a large number of STD pathogens which can generate coinfections, their simultaneous detection in a unique sample is very important. Multiplex polymerase chain reaction (PCR) is an advanced method of molecular biology which allows for simultaneous detection of multiple pathogens in the same sample. The advantages of the multiplex PCR method were assessed by various researchers by comparing the diagnosis results obtained with different other conventional methods. The sensitivity and specificity of these methods were analyzed on different specimens in comparison to traditional methods, such as culture media or direct microscopic examination. These studies demonstrated beyond any doubt that the multiplex PCR system is highly effective in the detection of each of multiple STD pathogens depicted from a single specimen and argued for multiplex PCR superiority in terms of sensitivity and rapidity.",signatures:"Mihaela L. Vică, Horea V. Matei and Costel V. Siserman",downloadPdfUrl:"/chapter/pdf-download/52716",previewPdfUrl:"/chapter/pdf-preview/52716",authors:[{id:"189561",title:"Dr.",name:"Mihaela Laura",surname:"Vica",slug:"mihaela-laura-vica",fullName:"Mihaela Laura Vica"},{id:"192251",title:"Dr.",name:"Horea Vladi",surname:"Matei",slug:"horea-vladi-matei",fullName:"Horea Vladi Matei"},{id:"192252",title:"Dr.",name:"Costel Vasile",surname:"Siserman",slug:"costel-vasile-siserman",fullName:"Costel Vasile Siserman"}],corrections:null},{id:"52654",title:"A Real-Time PCR-Based Diagnostic Test for Organisms in Respiratory Tract Infection",doi:"10.5772/65740",slug:"a-real-time-pcr-based-diagnostic-test-for-organisms-in-respiratory-tract-infection",totalDownloads:2020,totalCrossrefCites:3,totalDimensionsCites:7,hasAltmetrics:0,abstract:"Respiratory tract infection, especially pneumonia, is a significant cause of morbidity and mortality worldwide. Although rapid and accurate identification of the pathogens and the corresponding treatment, which is based on the microbiological results, is required in the healthcare setting, the current clinical tests lack high sensitivity and flexibility. As of yet, a comprehensive approach has not been able to work these issues out. Meanwhile, the development of molecular techniques enables the detection of organisms from respiratory specimens speedily as well as precisely and aids the settlement of such issues. With our novel approach that employs relative quantification, we successfully set the cutoff value to discriminate the causative pathogen from colonizing commensal organisms by real-time PCR. In this way, a diagnostic system for respiratory pathogens was devised and validated through clinical sample testing. In this chapter, a real-time PCR-based test capable of differentiating causative pathogens in respiratory specimens is described, and also its principle and the utility of this approach are illustrated.",signatures:"Takashi Hirama",downloadPdfUrl:"/chapter/pdf-download/52654",previewPdfUrl:"/chapter/pdf-preview/52654",authors:[{id:"189067",title:"Dr.",name:"Takashi",surname:"Hirama",slug:"takashi-hirama",fullName:"Takashi Hirama"}],corrections:null},{id:"52868",title:"PCR Technique for the Microbial Analysis of Inanimate Hospital Environment",doi:"10.5772/65742",slug:"pcr-technique-for-the-microbial-analysis-of-inanimate-hospital-environment",totalDownloads:1942,totalCrossrefCites:4,totalDimensionsCites:7,hasAltmetrics:0,abstract:"Discipline of molecular ecology and molecular techniques such as polymerase chain reaction (PCR) offers a possibility to study and reveal the microbial diversity in environmental settings with complicated mixed communities, non-culturable organisms, interfering contaminants and low levels of target DNA. Hospital environment represents a new ecological niche for clinically important nosocomial pathogens and antibiotic-resistant microorganisms, which have been commonly found on various hospital surfaces. Accurate characterization of microbial communities depends on several factors, starting with sample collection and conditional enrichment step. In the step of nucleic acid isolation and purification, the DNA, as a dominant signature molecule, is extracted followed by removing co-extracted impurities. PCR target sequences are often 16S rDNA gene, functional gene probes or species-specific probes, depending on the objective of the study. Furthermore, properly prepared PCR amplicons can serve as a basis for characterizing microbial community. The PCR technique is a powerful tool for the analysis of microbial diversity of environmental ecosystems. In a hospital environment, advantages of detecting pathogens and antibiotic-resistant bacteria need to be pointed out.",signatures:"Urška Rozman and Sonja Šostar Turk",downloadPdfUrl:"/chapter/pdf-download/52868",previewPdfUrl:"/chapter/pdf-preview/52868",authors:[{id:"189776",title:"Ph.D.",name:"Urška",surname:"Rozman",slug:"urska-rozman",fullName:"Urška Rozman"}],corrections:null},{id:"52972",title:"PCR: A Powerful Method in Food Safety Field",doi:"10.5772/65738",slug:"pcr-a-powerful-method-in-food-safety-field",totalDownloads:2394,totalCrossrefCites:1,totalDimensionsCites:4,hasAltmetrics:1,abstract:"In this chapter, application of the polymerase chain reaction (PCR) technique in food safety, considering all the branches of this concept, is presented. The area of interest contains important analysis for both human health and the identification of food adulteration. PCR techniques used for detection of genetically modified organisms (GMO) in different matrices, identification of different animal species in meat and dairy products, as well as the detection of food infection with food-borne pathogens and toxicogenic fungi are described. The working methods and result analysis are exemplified, starting with DNA isolation adjusted to different matrices, detection of target genes, and validation for all of these methods. Techniques of simplex PCR, primer multiplexing, primer design, validation of the laboratory methods, optimization of the PCR results, and result interpretation through the analysis of the electrophoresis gels and sequencing data are studied. At the same time, the obtained results, the obstacles encountered, and how they were overcome could be an example for specific analysis developed with less resources and also for adapting the existent validated methods to the new laboratory conditions. The practical applicability and the consumer’s demands are of great importance and always must be considered in developing and validating those methods.",signatures:"Oana-Maria Boldura and Sorina Popescu",downloadPdfUrl:"/chapter/pdf-download/52972",previewPdfUrl:"/chapter/pdf-preview/52972",authors:[{id:"189429",title:"Prof.",name:"Oana-Maria",surname:"Boldura",slug:"oana-maria-boldura",fullName:"Oana-Maria Boldura"},{id:"189451",title:"Prof.",name:"Sorina",surname:"Popescu",slug:"sorina-popescu",fullName:"Sorina Popescu"}],corrections:null},{id:"52975",title:"Site‐Directed Mutagenesis by Polymerase Chain Reaction",doi:"10.5772/66429",slug:"site-directed-mutagenesis-by-polymerase-chain-reaction",totalDownloads:4557,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"Since genomic data are widely available, many strategies have been implemented to reveal the function of specific nucleotides or amino acids in promoter regions or proteins, respectively. One of the methods most commonly used to determine the impact of mutations is the site‐directed mutagenesis using the polymerase chain reaction (PCR). There are different published protocols to develop single or multiple site‐directed mutagenesis. In this chapter, we reviewed the enzymes commonly used in site‐directed mutagenesis, the methods for simple and multiple site‐directed mutagenesis in large constructs, mediated by insertion of restriction sites. Other methods reviewed include high‐throughput site‐directed mutagenesis using oligonucleotides synthesized on DNA chips, and those based on multi‐site‐directed mutagenesis, based on recombination. Software tools to design site‐directed mutagenesis primers are also presented.",signatures:"Fabiola Castorena‐Torres, Katia Peñuelas‐Urquides and Mario\nBermúdez de León",downloadPdfUrl:"/chapter/pdf-download/52975",previewPdfUrl:"/chapter/pdf-preview/52975",authors:[{id:"188810",title:"Dr.",name:"Mario",surname:"Bermúdez De León",slug:"mario-bermudez-de-leon",fullName:"Mario Bermúdez De León"},{id:"188821",title:"Dr.",name:"Fabiola",surname:"Castorena Torres",slug:"fabiola-castorena-torres",fullName:"Fabiola Castorena Torres"},{id:"198351",title:"Dr.",name:"Katia",surname:"Peñuelas Urquides",slug:"katia-penuelas-urquides",fullName:"Katia Peñuelas Urquides"}],corrections:null}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"},subseries:null,tags:null},relatedBooks:[{type:"book",id:"7639",title:"Bioinformatics Tools for Detection and Clinical Interpretation of Genomic Variations",subtitle:null,isOpenForSubmission:!1,hash:"94f9f01b510ca80812f0eee467f9428b",slug:"bioinformatics-tools-for-detection-and-clinical-interpretation-of-genomic-variations",bookSignature:"Ali Samadikuchaksaraei and Morteza Seifi",coverURL:"https://cdn.intechopen.com/books/images_new/7639.jpg",editedByType:"Edited by",editors:[{id:"187501",title:"Prof.",name:"Ali",surname:"Samadikuchaksaraei",slug:"ali-samadikuchaksaraei",fullName:"Ali Samadikuchaksaraei"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"2553",title:"Lipid Peroxidation",subtitle:null,isOpenForSubmission:!1,hash:"b39734aa940b2d63ae5e8773d3dd5280",slug:"lipid-peroxidation",bookSignature:"Angel Catala",coverURL:"https://cdn.intechopen.com/books/images_new/2553.jpg",editedByType:"Edited by",editors:[{id:"196544",title:"Prof.",name:"Angel",surname:"Catala",slug:"angel-catala",fullName:"Angel Catala"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"2323",title:"Carbohydrates",subtitle:"Comprehensive Studies on Glycobiology and Glycotechnology",isOpenForSubmission:!1,hash:"f7c2e6a3566eee14c9884ad0820a6416",slug:"carbohydrates-comprehensive-studies-on-glycobiology-and-glycotechnology",bookSignature:"Chuan-Fa Chang",coverURL:"https://cdn.intechopen.com/books/images_new/2323.jpg",editedByType:"Edited by",editors:[{id:"145728",title:"Prof.",name:"Chuan-Fa",surname:"Chang",slug:"chuan-fa-chang",fullName:"Chuan-Fa Chang"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"372",title:"Aflatoxins",subtitle:"Biochemistry and Molecular Biology",isOpenForSubmission:!1,hash:"b7f7359995dc5ee04e12df282495f77e",slug:"aflatoxins-biochemistry-and-molecular-biology",bookSignature:"Ramón Gerardo Guevara-González",coverURL:"https://cdn.intechopen.com/books/images_new/372.jpg",editedByType:"Edited by",editors:[{id:"62559",title:"Dr.",name:"Ramon G.",surname:"Guevara-Gonzalez",slug:"ramon-g.-guevara-gonzalez",fullName:"Ramon G. Guevara-Gonzalez"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"2524",title:"Dehydrogenases",subtitle:null,isOpenForSubmission:!1,hash:"33ceb84d728f6a5033ba0389f83db79b",slug:"dehydrogenases",bookSignature:"Rosa Angela Canuto",coverURL:"https://cdn.intechopen.com/books/images_new/2524.jpg",editedByType:"Edited by",editors:[{id:"149584",title:"Prof.",name:"Rosa Angela",surname:"Canuto",slug:"rosa-angela-canuto",fullName:"Rosa Angela Canuto"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"2558",title:"Milk Protein",subtitle:null,isOpenForSubmission:!1,hash:"592dc83a9c30e990c850c0dec87d5d7a",slug:"milk-protein",bookSignature:"Walter L. Hurley",coverURL:"https://cdn.intechopen.com/books/images_new/2558.jpg",editedByType:"Edited by",editors:[{id:"136109",title:"Dr.",name:"Walter",surname:"Hurley",slug:"walter-hurley",fullName:"Walter Hurley"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"2290",title:"Protein-Protein Interactions",subtitle:"Computational and Experimental Tools",isOpenForSubmission:!1,hash:"5ad647ca09bc52a34fb2d02f38aa2455",slug:"protein-protein-interactions-computational-and-experimental-tools",bookSignature:"Weibo Cai and Hao Hong",coverURL:"https://cdn.intechopen.com/books/images_new/2290.jpg",editedByType:"Edited by",editors:[{id:"119750",title:"Dr.",name:"Weibo",surname:"Cai",slug:"weibo-cai",fullName:"Weibo Cai"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"2064",title:"Integrative Proteomics",subtitle:null,isOpenForSubmission:!1,hash:"82fe979a574466e287d597db196c0d67",slug:"integrative-proteomics",bookSignature:"Hon-Chiu Eastwood Leung, Tsz-Kwong Man and Ricardo J. Flores",coverURL:"https://cdn.intechopen.com/books/images_new/2064.jpg",editedByType:"Edited by",editors:[{id:"105582",title:"Dr.",name:"Hon-Chiu",surname:"Leung",slug:"hon-chiu-leung",fullName:"Hon-Chiu Leung"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"2555",title:"Lipoproteins",subtitle:"Role in Health and Diseases",isOpenForSubmission:!1,hash:"30f398afbe882fe1db3d1013952f3f13",slug:"lipoproteins-role-in-health-and-diseases",bookSignature:"Sasa Frank and Gerhard Kostner",coverURL:"https://cdn.intechopen.com/books/images_new/2555.jpg",editedByType:"Edited by",editors:[{id:"135586",title:"Prof.",name:"Gerhard",surname:"Kostner",slug:"gerhard-kostner",fullName:"Gerhard Kostner"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"4597",title:"Basic Principles and Clinical Significance of Oxidative Stress",subtitle:null,isOpenForSubmission:!1,hash:"89a2f0b8113514563d387fba14e963bd",slug:"basic-principles-and-clinical-significance-of-oxidative-stress",bookSignature:"Sivakumar Joghi Thatha Gowder",coverURL:"https://cdn.intechopen.com/books/images_new/4597.jpg",editedByType:"Edited by",editors:[{id:"118572",title:"Dr.",name:"Sivakumar Joghi",surname:"Thatha Gowder",slug:"sivakumar-joghi-thatha-gowder",fullName:"Sivakumar Joghi Thatha Gowder"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}}],ofsBooks:[]},correction:{item:{id:"79244",slug:"corrigendum-to-vascular-repair-and-remodeling-a-review",title:"Corrigendum to: Vascular Repair and Remodeling: A Review",doi:null,correctionPDFUrl:"https://cdn.intechopen.com/pdfs/79244.pdf",downloadPdfUrl:"/chapter/pdf-download/79244",previewPdfUrl:"/chapter/pdf-preview/79244",totalDownloads:null,totalCrossrefCites:null,bibtexUrl:"/chapter/bibtex/79244",risUrl:"/chapter/ris/79244",chapter:{id:"54438",slug:"vascular-repair-and-remodeling-a-review",signatures:"Nicolás F. Renna, Rodrigo Garcia, Jesica Ramirez and Roberto M.\nMiatello",dateSubmitted:"May 26th 2016",dateReviewed:"January 16th 2017",datePrePublished:null,datePublished:"April 5th 2017",book:{id:"5682",title:"Physiologic and Pathologic Angiogenesis",subtitle:"Signaling Mechanisms and Targeted Therapy",fullTitle:"Physiologic and Pathologic Angiogenesis - Signaling Mechanisms and Targeted Therapy",slug:"physiologic-and-pathologic-angiogenesis-signaling-mechanisms-and-targeted-therapy",publishedDate:"April 5th 2017",bookSignature:"Dan Simionescu and Agneta Simionescu",coverURL:"https://cdn.intechopen.com/books/images_new/5682.jpg",licenceType:"CC BY 3.0",editedByType:"Edited by",editors:[{id:"66196",title:"Dr.",name:"Dan",middleName:"T.",surname:"Simionescu",slug:"dan-simionescu",fullName:"Dan Simionescu"}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"}},authors:[{id:"192616",title:"Dr.",name:"Nicolás",middleName:null,surname:"Renna",fullName:"Nicolás Renna",slug:"nicolas-renna",email:"nicolasfede@gmail.com",position:null,institution:{name:"National University of Cuyo",institutionURL:null,country:{name:"Argentina"}}},{id:"202536",title:"Dr.",name:"Rodrigo",middleName:"Damián",surname:"García",fullName:"Rodrigo García",slug:"rodrigo-garcia",email:"rodridg@hotmail.com",position:null,institution:null},{id:"202537",title:"Dr.",name:"Jesica",middleName:null,surname:"Ramirez",fullName:"Jesica Ramirez",slug:"jesica-ramirez",email:"jesicamagali@hotmail.com",position:null,institution:null},{id:"202539",title:"Dr.",name:"Roberto M.",middleName:null,surname:"Miatello",fullName:"Roberto M. Miatello",slug:"roberto-m.-miatello",email:"rmmiatello@gmail.com",position:null,institution:null}]}},chapter:{id:"54438",slug:"vascular-repair-and-remodeling-a-review",signatures:"Nicolás F. Renna, Rodrigo Garcia, Jesica Ramirez and Roberto M.\nMiatello",dateSubmitted:"May 26th 2016",dateReviewed:"January 16th 2017",datePrePublished:null,datePublished:"April 5th 2017",book:{id:"5682",title:"Physiologic and Pathologic Angiogenesis",subtitle:"Signaling Mechanisms and Targeted Therapy",fullTitle:"Physiologic and Pathologic Angiogenesis - Signaling Mechanisms and Targeted Therapy",slug:"physiologic-and-pathologic-angiogenesis-signaling-mechanisms-and-targeted-therapy",publishedDate:"April 5th 2017",bookSignature:"Dan Simionescu and Agneta Simionescu",coverURL:"https://cdn.intechopen.com/books/images_new/5682.jpg",licenceType:"CC BY 3.0",editedByType:"Edited by",editors:[{id:"66196",title:"Dr.",name:"Dan",middleName:"T.",surname:"Simionescu",slug:"dan-simionescu",fullName:"Dan Simionescu"}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"}},authors:[{id:"192616",title:"Dr.",name:"Nicolás",middleName:null,surname:"Renna",fullName:"Nicolás Renna",slug:"nicolas-renna",email:"nicolasfede@gmail.com",position:null,institution:{name:"National University of Cuyo",institutionURL:null,country:{name:"Argentina"}}},{id:"202536",title:"Dr.",name:"Rodrigo",middleName:"Damián",surname:"García",fullName:"Rodrigo García",slug:"rodrigo-garcia",email:"rodridg@hotmail.com",position:null,institution:null},{id:"202537",title:"Dr.",name:"Jesica",middleName:null,surname:"Ramirez",fullName:"Jesica Ramirez",slug:"jesica-ramirez",email:"jesicamagali@hotmail.com",position:null,institution:null},{id:"202539",title:"Dr.",name:"Roberto M.",middleName:null,surname:"Miatello",fullName:"Roberto M. Miatello",slug:"roberto-m.-miatello",email:"rmmiatello@gmail.com",position:null,institution:null}]},book:{id:"5682",title:"Physiologic and Pathologic Angiogenesis",subtitle:"Signaling Mechanisms and Targeted Therapy",fullTitle:"Physiologic and Pathologic Angiogenesis - Signaling Mechanisms and Targeted Therapy",slug:"physiologic-and-pathologic-angiogenesis-signaling-mechanisms-and-targeted-therapy",publishedDate:"April 5th 2017",bookSignature:"Dan Simionescu and Agneta Simionescu",coverURL:"https://cdn.intechopen.com/books/images_new/5682.jpg",licenceType:"CC BY 3.0",editedByType:"Edited by",editors:[{id:"66196",title:"Dr.",name:"Dan",middleName:"T.",surname:"Simionescu",slug:"dan-simionescu",fullName:"Dan Simionescu"}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"}}},ofsBook:{item:{type:"book",id:"11816",leadTitle:null,title:"Latest Breakthroughs in Understanding and Treating Angioedema and Urticaria",subtitle:null,reviewType:"peer-reviewed",abstract:"
\r\n\tDespite being common dermatological disorders, angioedema and urticaria have been poorly understood throughout history. The real cause of developing their unpredictable debilitating symptoms has remained a mystery among doctors and scientists for years. However, the past decade was a turning point in uncovering the pathophysiology of these entities and discovering new treatments for cases that are resistant to conventional therapy. This book will discuss the most recent breakthroughs in the molecular mechanism of angioedema and urticaria, while providing updates on the newest possible etiologies and causes of these conditions, including hereditary angioedema with or without C1 inhibitor deficiency, FXII-related malfunctions, and chronic spontaneous urticaria (CSU). Moreover, the book intends to underline the latest treatments that have revolutionized the management of these diseases, with a focus on the different rising therapies in resistant cases, especially the newest monoclonal antibodies. This will update dermatologists and physicians on the myriad options we can use in cases that were once considered impossible to treat.
",isbn:"978-1-83969-228-4",printIsbn:"978-1-83969-227-7",pdfIsbn:"978-1-83969-232-1",doi:null,price:0,priceEur:0,priceUsd:0,slug:null,numberOfPages:0,isOpenForSubmission:!0,isSalesforceBook:!1,isNomenclature:!1,hash:"0315e9d2989fad5f055e4ad856f77b76",bookSignature:"Dr. Charbel Skayem and Dr. Tu Anh Duong",publishedDate:null,coverURL:"https://cdn.intechopen.com/books/images_new/11816.jpg",keywords:"Hereditary, C1 Inhibitor, C1q, IgE-Mediated, Non-IgE-Mediated, Histamine, Degranulation, Mast Cells, Neuropeptide, Hypersensitivity, Auto-Immune, Anti-histamine",numberOfDownloads:null,numberOfWosCitations:0,numberOfCrossrefCitations:null,numberOfDimensionsCitations:null,numberOfTotalCitations:null,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"April 20th 2022",dateEndSecondStepPublish:"June 29th 2022",dateEndThirdStepPublish:"August 28th 2022",dateEndFourthStepPublish:"November 16th 2022",dateEndFifthStepPublish:"January 15th 2023",dateConfirmationOfParticipation:null,remainingDaysToSecondStep:"17 hours",secondStepPassed:!0,areRegistrationsClosed:!1,currentStepOfPublishingProcess:3,editedByType:null,kuFlag:!1,biosketch:"Dr. Charbel Skayem is a dermatologist and venereologist in Assistance Publique-Hôpitaux de Paris(AP-HP), the largest university hospital system in Europe, and Gustave Roussy, the first cancer research hospital in Europe and among the top 5 best-specialized hospitals in the world. Dr. Skayem graduated from the Sorbonne University, University of Paris, and Paris-Saclay University, and is a member of several dermatological societies and academies, and the author of numerous articles in top leading journals.",coeditorOneBiosketch:"Dermatologist in Assistance Publique des Hôpitaux de Paris (AP-HP), the largest university hospital system in Europe, Paris-Saclay University, and a numeric design engineer in medicine. President of teledermatology in the French Society of dermatology and coordinator of TELDERM AP-HP.",coeditorTwoBiosketch:null,coeditorThreeBiosketch:null,coeditorFourBiosketch:null,coeditorFiveBiosketch:null,editors:[{id:"461547",title:"Dr.",name:"Charbel",middleName:null,surname:"Skayem",slug:"charbel-skayem",fullName:"Charbel Skayem",profilePictureURL:"https://mts.intechopen.com/storage/users/461547/images/system/461547.jpg",biography:"Dr Charbel Skayem is a dermatologist, venereologist, and dermatologic surgeon practicing in Assistance Publique des Hôpitaux de Paris (AP-HP), the largest university hospital system in Europe and one of the largest in the world, and in Gustave Roussy, the first cancer-research hospital in Europe and among the top 5 best specialized university hospitals in the world. Lebanese in origin, he moved to Paris to pursue his residency in dermatology and venereology, followed by several fellowships, graduating from the Sorbonne University, the University of Paris, and Paris-Saclay University. His initial experiences in different worldwide-renowned pioneer referral departments have given him a wide expertise in multiple branches of his specialty: oncodermatology, advanced dermatologic surgery, inflammatory and infectious dermatology, teledermatology, sexually transmitted diseases, and cosmetic and laser dermatology. Dr Skayem is a member of different dermatological societies and academies. He was a laureate of several scholarships and awards. He was a distinguished speaker in many global congresses. Despite his youth, he served as a reviewer and editor, has written several chapters in books, and numerous articles in leading peer-reviewed medical journals.",institutionString:"Assistance Publique -Hopitaux De Paris",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"0",totalChapterViews:"0",totalEditedBooks:"0",institution:{name:"Assistance Publique -Hopitaux De Paris",institutionURL:null,country:{name:"France"}}}],coeditorOne:{id:"462437",title:"Dr.",name:"Tu Anh",middleName:null,surname:"Duong",slug:"tu-anh-duong",fullName:"Tu Anh Duong",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y00003QRGVyQAP/Profile_Picture_2022-04-21T07:34:55.png",biography:"Dr Tu Anh Duong is a dermatologist in Assistance Publique des Hôpitaux de Paris (AP-HP), the largest hospital system in Europe, Paris-Saclay University, and a numeric design engineer in medicine. Affiliated to leading referral departments in dermatology (in toxic epidermal necrolysis, bullous dermatological diseases, severe cutaneous adverse reactions, dermatological emergencies, and neurofibromatosis), Dr. Duong has wide expertise in severe skin disorders. She is an innovator in the field of teledermatology and is the president of teledermatology in the French Society of dermatology, the coordinator of TELDERM AP-HP, and Chair Avenir Santé Numérique. Her innovations and interest fields lie in dermatological emergencies and engineering dermatological numeric transformations and conversions. Dr. Duong is the author of hundreds of articles in leading peer-reviewed medical journals.",institutionString:"Assistance Publique -Hopitaux De Paris",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"0",totalChapterViews:"0",totalEditedBooks:"0",institution:{name:"Assistance Publique -Hopitaux De Paris",institutionURL:null,country:{name:"France"}}},coeditorTwo:null,coeditorThree:null,coeditorFour:null,coeditorFive:null,topics:[{id:"16",title:"Medicine",slug:"medicine"}],chapters:null,productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"},personalPublishingAssistant:{id:"466998",firstName:"Dragan",lastName:"Miljak",middleName:"Anton",title:"Mr.",imageUrl:"https://mts.intechopen.com/storage/users/466998/images/21564_n.jpg",email:"dragan@intechopen.com",biography:"As an Author Service Manager my responsibilities include monitoring and facilitating all publishing activities for authors and editors. From chapter submission and review, to approval and revision, copy-editing and design, until final publication, I work closely with authors and editors to ensure a simple and easy publishing process. A unique name with a unique work ethic right at your service."}},relatedBooks:[{type:"book",id:"6550",title:"Cohort Studies in Health Sciences",subtitle:null,isOpenForSubmission:!1,hash:"01df5aba4fff1a84b37a2fdafa809660",slug:"cohort-studies-in-health-sciences",bookSignature:"R. Mauricio Barría",coverURL:"https://cdn.intechopen.com/books/images_new/6550.jpg",editedByType:"Edited by",editors:[{id:"88861",title:"Dr.",name:"R. Mauricio",surname:"Barría",slug:"r.-mauricio-barria",fullName:"R. Mauricio Barría"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"9500",title:"Recent Advances in Bone Tumours and Osteoarthritis",subtitle:null,isOpenForSubmission:!1,hash:"ea4ec0d6ee01b88e264178886e3210ed",slug:"recent-advances-in-bone-tumours-and-osteoarthritis",bookSignature:"Hiran Amarasekera",coverURL:"https://cdn.intechopen.com/books/images_new/9500.jpg",editedByType:"Edited by",editors:[{id:"67634",title:"Dr.",name:"Hiran",surname:"Amarasekera",slug:"hiran-amarasekera",fullName:"Hiran Amarasekera"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"1591",title:"Infrared Spectroscopy",subtitle:"Materials Science, Engineering and Technology",isOpenForSubmission:!1,hash:"99b4b7b71a8caeb693ed762b40b017f4",slug:"infrared-spectroscopy-materials-science-engineering-and-technology",bookSignature:"Theophile Theophanides",coverURL:"https://cdn.intechopen.com/books/images_new/1591.jpg",editedByType:"Edited by",editors:[{id:"37194",title:"Dr.",name:"Theophile",surname:"Theophanides",slug:"theophile-theophanides",fullName:"Theophile Theophanides"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"3161",title:"Frontiers in Guided Wave Optics and Optoelectronics",subtitle:null,isOpenForSubmission:!1,hash:"deb44e9c99f82bbce1083abea743146c",slug:"frontiers-in-guided-wave-optics-and-optoelectronics",bookSignature:"Bishnu Pal",coverURL:"https://cdn.intechopen.com/books/images_new/3161.jpg",editedByType:"Edited by",editors:[{id:"4782",title:"Prof.",name:"Bishnu",surname:"Pal",slug:"bishnu-pal",fullName:"Bishnu Pal"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"3092",title:"Anopheles mosquitoes",subtitle:"New insights into malaria vectors",isOpenForSubmission:!1,hash:"c9e622485316d5e296288bf24d2b0d64",slug:"anopheles-mosquitoes-new-insights-into-malaria-vectors",bookSignature:"Sylvie Manguin",coverURL:"https://cdn.intechopen.com/books/images_new/3092.jpg",editedByType:"Edited by",editors:[{id:"50017",title:"Prof.",name:"Sylvie",surname:"Manguin",slug:"sylvie-manguin",fullName:"Sylvie Manguin"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"371",title:"Abiotic Stress in Plants",subtitle:"Mechanisms and Adaptations",isOpenForSubmission:!1,hash:"588466f487e307619849d72389178a74",slug:"abiotic-stress-in-plants-mechanisms-and-adaptations",bookSignature:"Arun Shanker and B. Venkateswarlu",coverURL:"https://cdn.intechopen.com/books/images_new/371.jpg",editedByType:"Edited by",editors:[{id:"58592",title:"Dr.",name:"Arun",surname:"Shanker",slug:"arun-shanker",fullName:"Arun Shanker"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"72",title:"Ionic Liquids",subtitle:"Theory, Properties, New Approaches",isOpenForSubmission:!1,hash:"d94ffa3cfa10505e3b1d676d46fcd3f5",slug:"ionic-liquids-theory-properties-new-approaches",bookSignature:"Alexander Kokorin",coverURL:"https://cdn.intechopen.com/books/images_new/72.jpg",editedByType:"Edited by",editors:[{id:"19816",title:"Prof.",name:"Alexander",surname:"Kokorin",slug:"alexander-kokorin",fullName:"Alexander Kokorin"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"314",title:"Regenerative Medicine and Tissue Engineering",subtitle:"Cells and Biomaterials",isOpenForSubmission:!1,hash:"bb67e80e480c86bb8315458012d65686",slug:"regenerative-medicine-and-tissue-engineering-cells-and-biomaterials",bookSignature:"Daniel Eberli",coverURL:"https://cdn.intechopen.com/books/images_new/314.jpg",editedByType:"Edited by",editors:[{id:"6495",title:"Dr.",name:"Daniel",surname:"Eberli",slug:"daniel-eberli",fullName:"Daniel Eberli"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"57",title:"Physics and Applications of Graphene",subtitle:"Experiments",isOpenForSubmission:!1,hash:"0e6622a71cf4f02f45bfdd5691e1189a",slug:"physics-and-applications-of-graphene-experiments",bookSignature:"Sergey Mikhailov",coverURL:"https://cdn.intechopen.com/books/images_new/57.jpg",editedByType:"Edited by",editors:[{id:"16042",title:"Dr.",name:"Sergey",surname:"Mikhailov",slug:"sergey-mikhailov",fullName:"Sergey Mikhailov"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"1373",title:"Ionic Liquids",subtitle:"Applications and Perspectives",isOpenForSubmission:!1,hash:"5e9ae5ae9167cde4b344e499a792c41c",slug:"ionic-liquids-applications-and-perspectives",bookSignature:"Alexander Kokorin",coverURL:"https://cdn.intechopen.com/books/images_new/1373.jpg",editedByType:"Edited by",editors:[{id:"19816",title:"Prof.",name:"Alexander",surname:"Kokorin",slug:"alexander-kokorin",fullName:"Alexander Kokorin"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}}]},chapter:{item:{type:"chapter",id:"62636",title:"Evaluation of Retinal Nerve Fiber Layer and Inner Macular Layers in Primary Open-Angle Glaucoma with Spectral-Domain Optical Coherence Tomography",doi:"10.5772/intechopen.79102",slug:"evaluation-of-retinal-nerve-fiber-layer-and-inner-macular-layers-in-primary-open-angle-glaucoma-with",body:'\nAccording to the American Academy of Ophthalmology the medical term glaucoma is used for a group of diseases that damage the optic nerve (ON) as distinctive type of optic neuropathy characterized by structural (cupping of optic nerve head—ONH, changes in connective tissue structural elements and number of the nerve fibers in ON) and functional changes (typical visual field defects). Increased intraocular pressure (IOP) is one of the most common risk factors associated with developing and progressing of the disease, but its presence or absence does not change the above-mentioned glaucoma definition [1].
\nEpidemiological studies found that the glaucoma at the end of twentieth century covered more than 60 million people around the world. Prognostic studies show an increasing trend of the number of affected patients, and in 2020 it is going to be approximately 80 million people, and in 2040 approximately 112 million [2, 3, 4]. Cataract and glaucoma are leading causes of blindness worldwide. Because of the reversibility of the vision after cataract extraction, the glaucoma remains the leading cause of irreversible blindness. The large number of glaucoma patients, irreversible vision loss and the impact on the life quality of the affected people are just part of the reasons for making glaucoma one of the diseases with big social influence.
\nGlaucoma is characterized by irreversible loss of ganglion cells, which axons form the ON. Ganglion cells are localized in three retinal layers—inner plexiform layer or IPL (their dendrites), ganglion cell layer or GCL (their bodies), and retinal nerve fiber layer or RNFL (their axons). Therefore exactly the above-mentioned layers are those, which glaucoma affects accompanying typical visual field defects [5]. Chronic and progressive loss of neuroretinal tissue is cardinal feature of glaucomatous optic neuropathy (GON) and criterion for diagnosis [6].
\nAll afferent pathways in the ON start from a layer of photoreceptors (cones and rods), which is located in the retina on area more than 1000 mm2. In ONH all fibers are concentrated on surface with approximately 2–3 mm2 area [7, 8]. From the body of each ganglion cell comes out a nerve fiber or axon, which moves toward the ONH. So that it can be called conglomerate consisted of all converging axons, which are as mentioned above part of the retina and form a layer—RNFL [9]. Nowhere else the ganglions’ nerve fibers are not so much compact as they are in ONH, and this is what determines the importance of peripapillary RNFL thickness in diagnosis and follow up of patients with GON.
\nThese are some characteristics of RNFL [9, 10]:
Papillomacular nerve fiber bundle—it starts from ganglion cells in the foveolar region. The nerve fibers from nasal foveolar area move straight toward the temporal border of ONH, and those from the temporal part make a slight arc around the nasal nerve fibers and then join to the straight bundle.
Superior and inferior retinal arcades—they are created by later formed nerve fibers and ganglion cells originating temporal to the fovea. They arc around the macula and papillomacular bundle to enter the ONH.
Temporal raphe or seam—it extends from the fovea to the temporal part of the retina and consists of very few axons, because by rule the nerve fibers from the upper half of the retina do not pass the horizontal meridian to the arcuate course of the nerve fibers from the lower part of the retina, and vice versa.
An extremely large collection of nerve fibers in the superior and inferior quadrant of the ONH—namely these two regions are set to be more vulnerable for glaucomatous damages.
Nasal nerve fibers—they move radially toward the ONH.
Exact location of the nerve fibers in the ONH according to their position in the fundus—the more peripheral retinal location the more central ONH localization.
Basic features, used to make an assessment of RNFL images [9]:
Striations of RNFL—normally RNFL can be seen as striated bright and dark lines in the areas of superior and inferior temporal blood vessels in healthy eyes. If atrophy is presented (<50 μm RNFL thickness) the striations of the background disappear and bright lines cannot be seen because of the RNFL loss [11] (see Figure 1).
Defects of the background brightness—they can be diffuse loss and localized defects (wedge-shaped and cleft-shaped). The width in the cleft-shaped defects is the same along the full length, however the width in wedge-shaped defects is different, peripherally they are wider and become narrower toward the ONH. This could be explained with convergent course of the nerve fibers. Diffuse defects have an impact over the complete RNFL thickness in the fundus and also their diagnosis is more difficult from localized defects.
Visualization of the blood vessels—normally RNFL covers retinal blood vessels. That’s why small and medium-sized blood vessels have unclear contours and look misty. When RNFL atrophy appears, then blood vessels can be seen clearly because of the less covering from the nerve fiber layer.
RNFL striations in superior temporal area of the fundus in healthy eye of 52 years old female.
All nerve fibers are arranged in a specific way in the ONH not only in each and every human being but also in each and every of the human eyes. Equal quantity nerve fibers may look in a different way in the borders of ONHs with dissimilar disc area, depth of the lamina cribrosa, and height of the scleral canal [12]. Equal functional capacitate could be presented by different looking structures and vice versa—equal looking structures could have different functional activity [13, 14, 15].
\nThe RNFL thickness depends on: age, ethnicity, number and thickness of the nerve fibers, quantity of the glia, quantity of the blood vessels, disk area of the ONH, axial length of the eye (Ax). The thickness of the measured RNFL depends also on: the stage of peripapillary atrophy/conus myopicus, vitreoretinal tractions. The excavation (cupping) depends on: disc area, number and thickness of the nerve fibers, quantity of the glia [14]. Normally in the course of time the RNFL thickness decreases with age normally with 4000–5000 axons per year [16, 17, 18, 19] and this is approximately 2.0 μm/decade or 0.2% per year at mean thickness 100 μm [20]. The ON consists of 700,000–1.4 million nerve fibers and the RNFL thickness in healthy people has a wide variety of a norm. The usage of absolute values restricts the process of distinguishing healthy from glaucoma patients [20]. Therefore some authors talk about “modulation of RNFL thickness”—it shows the relative loss of nerve fibers as difference between the biggest and smallest measured value of RNFL thickness in a retinal region of interest [21].
\nWhen assessing glaucomatous damages it is appropriate to measure the RNFL thickness, because thinning of this layer correlated directly with ganglion cells loss, which is the basic pathophysiological event [22]. Evaluation of the RNFL thickness is important for early glaucoma diagnosis before appearing of the clinical manifestations of the disease. It is proven that 40–50% of the nerve fibers are should be dropped out before developing of the visual field defects [23]. Clinical evaluation of the RNFL with red-free photography shows that thinning of the layer can be seen in 60% of the pictures 6 years before appearing of clinical manifestation of the defects in visual field [24]. These facts show that structural changes occur before the functional ones. Typical visual field defects in glaucoma are nasal step, arcuate scotoma, paracentral scotoma, generalized depression, and progressive worsening of the indices of the standard automated perimetry (SAP) [25].
\nSometimes in glaucoma visual field defects can be seen without appearance of structural glaucomatous changes. It is possible also in equal RNFL loss to be obtained a different clinical finding according to initial RNFL thickness. This could be explained with the following: visual field defects appear after 40% loss of the nerve fibers. Each man is born with different quantity nerve fibers. If a person owns very thick for human population RNFL, the loss of 40% nerve fibers probably will not give any significant results in optical coherence tomography (OCT)—the line thickness will be in the middle of the green zone, the zone shows lack of disease. Then this individual is going to have functional defects with normal RNFL thickness. If another person is born with thin RNFL, the loss of 40% nerve fibers will give significant results—OCT line thickness will be close to the yellow zone or in the zone. Then this individual is going to have functional defects with pathological thin RNFL [14, 15].
\nIn the early glaucoma stage it is considered that the affected ganglion cells decrease their functional processes before they die and this leads to decreasing of the visual functions without an obvious structural changes. This is the reason why a patient has functional manifestations of glaucoma in combination with normal RNFL thickness [14].
\nThe most distant nerve fibers from ONH originate exactly from these farthest parts of the ganglion cells in the retina and they are located deeply in the RNFL. They pass closely to the scleral edge and most peripherally in the ON [26]. These nerve fibers that originate from the closest to the ONH parts of the retina are located superficially in the RNFL and pass centrally in the ON. It is thought that the nerve fibers, which are located superficially in the RNFL, are more vulnerable in glaucoma, and their damage is associated with an enlargement of the blind spot.
\nIt is also believed that chronically increased IOP leads to compression of the circulation of the Elschnig’s border tissue and its atrophy. Then lamina cribrosa starts posteriorization. It is considered therefore that it is a reason for stretching and rupturing of the nerve fibers which are closest to the scleral edge. Only nerve fibers in prelaminar region can drop out consequently, because they are separated and not in bundles. The affecting of the nerve fibers is from peripheral to central region [26, 27]. Unordered affecting of the nerve fibers can be seen in acute angle closure glaucoma.
\nThe aim of our research is to assess and compare the peripapillary RNFL (pRNFL) thickness diagnostic capability with those of three macular parameters—macular RNFL (mRNFL) thickness, GCL+ (ganglion cell layer with inner plexiform layer thickness), and GCL++ (mRNFL and GCL+) in primary open-angle glaucoma patients with spectral-domain OCT (SD-OCT).
\nAll participants (healthy volunteers and patients) included in current clinical study were examined in the university eye clinic of Alexandrovska Hospital, Sofia, Bulgaria for total period of time—a year and 3 months. This is a prospective observational study of 414 participants (483 eyes) aged 45–84 years (mean 66.7 ± 8.7), male—132, and female—282. All patients were distributed into six groups:
\nIst group (
IInd group (
IIIrd group (
IVth group (
Vth group (
VIth group (
The following inclusion and exclusion criteria were defined for the groups:
\nInclusion criteria for the control group: healthy participants without congenital or acquired general or eye diseases exception of early age-related cataract; people without family history and other risk factors for glaucoma; best corrected visual acuity (BCVA) = 1.0; refraction error in ±4.00 dsph and ±1.00 dcyl; IOP under 21 mmHg measured with Goldmann tonometer according to central corneal thickness (CCT) values; open anterior chamber angle class III–IV Shaffer Angle Classification System; ocular fundus without glaucomatous damages—vital optic nerve head (ONH), ISNT rule in norm, C/D Ratio < 0.5 PD and interocular asymmetry in C/D Ratio ≤ 0.2 PD; normal SAP (Glaucoma Hemifield Test—within normal limits, p ˃ 0.05 for MD and PSD indices).
\nInclusion criteria for OH group: patients with OH and any other coexisting ocular and general pathology; BCVA = 1.0; refraction error in ±4.00 dsph and ±1.00 dcyl; permanent elevation of IOP more than 21 mmHg measured with Goldmann tonometer without treatment and corrected according to the CCT values and daytime pressure curves; open anterior chamber angle; lack of pathological changes in the fundus; normal SAP.
\nInclusion criteria for Preperimetric glaucoma group: BCVA = 1.0; refraction error in already shown limits; permanent elevation of IOP more than 21 mmHg; open anterior chamber angle; fundus glaucomatous changes: interocular asymmetry in C/D Ratio ≥ 0.2 PD, vertical elongated excavation, thinning of optic disc rim, local thinning of neuroretinal rim, violated ISNT rule, defects in RNFL thickness (diffuse or local), normal SAP.
\nInclusion criteria for perimetric glaucoma groups: BCVA = 1.0 for early stage glaucoma group and BCVA ≥ 0.2 for moderate and advanced stage of POAG; refraction error in already shown limits; permanent elevation of IOP more than 21 mmHg; open anterior chamber angle; fundus glaucomatous changes: interocular asymmetry in C/D Ratio ≥ 0.2 PD, vertical elongated excavation, thinning of optic disc rim, local thinning of neuroretinal rim, violated ISNT rule, defects in RNFL thickness (diffuse or local), ONH hemorrhages; typical for glaucoma visual field defects in SAP corresponding with changes in ONH; glaucoma perimetric stage was defined as changes in SAP based on Hodapp-Parrish-Anderson classification.
\nExclusion criteria: best corrected visual acuity ≤ 0.2; age < 45 years and > 85 years; refraction error beyond already shown limits; normotensive glaucoma, angle closure glaucoma; macular pathology, diabetic retinopathy, nonglaucomatous opticopathy; previous eye surgery (exception cataract refractive surgery with intraocular lens implantation); coexisting neurological pathology which can influence on the visual field results.
\nAll patients underwent full ophthalmological examination including: a complete case history for eye and general diseases; family history; refraction and best corrected visual acuity; slit-lamp examination; indirect fundus biomicroscopy; contact ultrasound pachymetry (OcuScan RxP - Alcon, Forth Worth, Texas, USA); Goldmann tonometry; indirect gonioscopy (Goldmann three-mirror gonioprism/Shaffer classification, 1960); SAP - SITA Standard 24-2, HFA II (Carl Zeiss Meditec, Dublin, CA, USA) with near correction if necessary. Only reliable perimetry results with total error rate (loss of fixation and false-positive and false-negative results) lower than 25%. The stage of POAG changes was determined using Hodapp-Parrish-Anderson classification.
\nOptical coherence tomography: All patients underwent SD-ОСТ of both eyes with dilated pupils by one examiner using Topcon 3D OCT 2000 (FA plus) (Topcon Corporation, Japan), software version - 8.11.
\nThe following programs were used:
Circle program evaluated peripapillary RNFL thickness. From Circle protocol we analyzed the following parameters: (1) Total pRNFL—showed the average thickness in 360°; (2) Sup pRNFL—showed the thickness in the superior 90°; (3) Inf pRNFL—showed the thickness in the inferior 90°; (4) Nas pRNFL—showed the thickness in the nasal 90°; (5) Temp pRNFL—showed the thickness in the temporal 90° (see Figure 2, right).
3D Macula (V) program is used for the internal macular layers thickness evaluation in area of 7 mm2. The following parameters were analyzed: (1) Sup mRNFL (Sup mRNFL)—mRNFL thickness in the upper half; (2) Inf mRNFL (Inf mRNFL)—in the lower half; (3) Total mRNFL (Total mRNFL)—in the whole macular area (see Figure 2, left).
Glaucoma analysis—Macula protocol (left) and Circle protocol (right).
Only OCT protocols with scan quality over 50%, no artifacts from eye or body movements, blinking, and lack of macular pathology (edema, drusen, holes) were included in the analysis.
\nStatistical methods: For statistically significant were considered the differences with P values <0.05. We used descriptive, dispersion and ROC-analysis to evaluate diagnostic accuracy, specificity, and sensitivity. A comparison was made between Ist group and IInd, Ist and IIIrd and so long. With comparison analysis we searched for statistical significant difference between some of the parameters’ values in specificity and sensitivity.
\nThe descriptive statistics can be seen in Table 1, and mean values of the all RNFL parameters in Table 2. In Table 3 can be seen the ROC analysis and the diagnostic capabilities of the eight OCT parameters in each group. The RNFL parameter with highest diagnostic potential in the groups—PPG (AUROC = 0.879), early (AUROC = 0.929), moderate (AUROC = 0.989) and advanced glaucoma (AUROC = 1.000) is Total mRNFL followed by Inf mRNFL and Inf pRNFL. The RNFL parameters with lowest diagnostic potential in all glaucoma stages are Nas pRNFL, Temp RNFL. A single RNFL parameter (Total mRNFL) was measured with highest diagnostic accuracy for glaucoma in all of its stages - from PPG to advanced glaucoma. In the current investigation, it is shown for the first time the higher diagnostic ability of macular RNFL from those of peripapillary RNFL. In OH group, we found that Inf mRNFL has the highest diagnostic possibilities but without any clinical significance, because none of the RNFL parameters change significantly in patients with OH in comparison with control group.
\nGroup | \nSex | \nNumber | \nAge (years) | \n|||
---|---|---|---|---|---|---|
Mean | \nSD | \nMin | \nMax | \n|||
Controls | \nMen—m | \n30 | \n61.7 | \n9.7 | \n48.0 | \n81.0 | \n
Women—f | \n120 | \n63.4 | \n8.9 | \n45.0 | \n84.0 | \n|
All | \n150 | \n63.0 | \n9.0 | \n45.0 | \n84.0 | \n|
OH | \nm | \n10 | \n59.4 | \n10.6 | \n45.0 | \n76.0 | \n
f | \n21 | \n60.4 | \n8.6 | \n45.0 | \n72.0 | \n|
All | \n31 | \n60.1 | \n9.2 | \n45.0 | \n76.0 | \n|
Preperimetric glaucoma | \nm | \n20 | \n68.6 | \n6.9 | \n51.0 | \n81.0 | \n
f | \n29 | \n64.7 | \n7.6 | \n45.0 | \n74.0 | \n|
All | \n49 | \n66.3 | \n7.5 | \n45.0 | \n81.0 | \n|
Early glaucoma | \nm | \n30 | \n71.7 | \n6.0 | \n58.0 | \n81.0 | \n
f | \n50 | \n68.5 | \n8.7 | \n45.0 | \n82.0 | \n|
All | \n80 | \n69.7 | \n7.9 | \n45.0 | \n82.0 | \n|
Moderate glaucoma | \nm | \n11 | \n70.5 | \n10.2 | \n45.0 | \n82.0 | \n
f | \n23 | \n70.4 | \n7.9 | \n57.0 | \n81.0 | \n|
All | \n34 | \n70.4 | \n8.5 | \n45.0 | \n82.0 | \n|
Advanced glaucoma | \nm | \n31 | \n67.7 | \n11.5 | \n45.0 | \n83.0 | \n
f | \n39 | \n70.7 | \n8.2 | \n45.0 | \n84.0 | \n|
All | \n70 | \n69.5 | \n9.8 | \n45.0 | \n84.0 | \n
Descriptive statistics.
Parameter (μm) | \nControls | \nOH | \nPreperimetric glaucoma | \nEarly glaucoma | \nModerate glaucoma | \nAdvanced glaucoma | \n
---|---|---|---|---|---|---|
Mean ± SD | \nMean ± SD | \nMean ± SD | \nMean ± SD | \nMean ± SD | \nMean ± SD | \n|
MD [dB] | \n−0.24 ± 1.30 | \n−0.05 ± 1.15 | \n−0.60 ± 1.13 | \n−2.73 ± 1.85 | \n−8.65 ± 1.77 | \n−21.44 ± 5.81 | \n
PSD [dB] | \n1.72 ± 0.38 | \n1.59 ± 0.32 | \n1.82 ± 0.33 | \n3.72 ± 1.73 | \n7.84 ± 2.66 | \n9.26 ± 3.15 | \n
Sup mRNFL | \n36.09 ± 4.30 | \n36.46 ± 5.77 | \n30.58 ± 3.75 | \n28.90 ± 4.90 | \n24.60 ± 6.85 | \n16.78 ± 6.58 | \n
Inf mRNFL | \n39.22 ± 5.27 | \n38.16 ± 4.69 | \n31.34 ± 5.01 | \n29.44 ± 5.81 | \n25.05 ± 7.04 | \n14.20 ± 6.33 | \n
Total mRNFL | \n37.67 ± 4.23 | \n37.30 ± 4.67 | \n31.05 ± 4.06 | \n29.21 ± 4.37 | \n25.00 ± 4.75 | \n15.48 ± 5.58 | \n
Sup pRNFL | \n122.31 ± 12.09 | \n128.70 ± 14.86 | \n111.34 ± 17.17 | \n101.47 ± 14.18 | \n90.38 ± 20.29 | \n77.47 ± 16.26 | \n
Inf pRNFL | \n136.86 ± 14.46 | \n137.66 ± 14.65 | \n115.74 ± 18.14 | \n108.05 ± 22.62 | \n90.53 ± 23.59 | \n69.22 ± 15.03 | \n
Nas pRNFL | \n90.55 ± 14.73 | \n92.24 ± 18.47 | \n81.98 ± 19.99 | \n82.23 ± 18.00 | \n74.58 ± 19.36 | \n66.99 ± 16.63 | \n
Temp pRNFL | \n81.62 ± 11.76 | \n85.92 ± 16.12 | \n74.26 ± 14.88 | \n72.98 ± 15.62 | \n69.28 ± 17.88 | \n60.35 ± 15.76 | \n
Total pRNFL | \n107.84 ± 7.95 | \n111.14 ± 10.25 | \n95.92 ± 12.26 | \n90.81 ± 12.48 | \n81.15 ± 15.28 | \n68.46 ± 12.32 | \n
Mean values and standard deviation (SD) of RNFL in all groups.
Parameter | \nOH | \nPreperimetric glaucoma | \nEarly glaucoma | \nModerate glaucoma | \nAdvanced glaucoma | \n
---|---|---|---|---|---|
AUROC | \nAUROC | \nAUROC | \nAUROC | \nAUROC | \n|
Sup pRNFL | \n0.364 | \n0.694 | \n0.866 | \n0.903 | \n0.983 | \n
Inf pRNFL | \n0.472 | \n0.820 | \n0.867 | \n0.957 | \n0.999 | \n
Nas pRNFL | \n0.486 | \n0.627 | \n0.643 | \n0.731 | \n0.874 | \n
Temp pRNFL | \n0.428 | \n0.678 | \n0.687 | \n0.719 | \n0.893 | \n
Total pRNFL | \n0.412 | \n0.791 | \n0.900 | \n0.947 | \n0.993 | \n
Sup mRNFL | \n0.514 | \n0.839 | \n0.886 | \n0.907 | \n0.996 | \n
Inf mRNFL | \n0.864 | \n0.907 | \n0.951 | \n0.997 | \n|
Total mRNFL | \n0.535 | \n
ROC-analysis.
AUROC values allowed us to create ROC curves in all groups as we included the five RNFL parameters with the best results (Figures 3, 4, 5, 6).
\nPPG.
Early glaucoma.
Moderate glaucoma.
Advanced glaucoma.
After that we used comparison analysis to demonstrate if statistical significant difference exists between diagnostic possibilities of RNFL parameters in all groups. In Table 4 can be seen only two significant differences in AUROC values between Sup mRNFL (0.907) and Total mRNFL, and between Total mRNFL (0.929) and Inf pRNFL (0.867). Although, we found the highest diagnostic potential in all glaucoma groups for Total mRNFL, our comparison analysis showed that these possibilities are not statistical significant with exception of the above-mentioned two examples. For instance, in the stage of PPG, we were not able to find any statistical significant differences between the best five diagnostic RNFL parameters (Table 4), so they have equal abilities to diagnose glaucoma patients in this particular stage. In the stage of early POAG, we found significant difference in diagnostic abilities between Inf pRNFL and Total mRNFL, so that it would be better if the clinician uses not the first five but the four best diagnostic parameters from Table 3.
\nAUROC comparisons | \nControls vs. | \n||||
---|---|---|---|---|---|
PPG | \nEarly glaucoma | \nModerate glaucoma | \nAdvanced glaucoma | \n||
p | \np | \np | \np | \n||
Sup mRNFL | \nInf mRNFL | \n0.587 | \n0.486 | \n0.280 | \n0.806 | \n
Sup mRNFL | \nTotal mRNFL | \n0.339 | \n0.136 | \n0.238 | \n|
Sup mRNFL | \nInf pRNFL | \n0.680 | \n0.571 | \n0.178 | \n0.469 | \n
Sup mRNFL | \nTotal pRNFL | \n0.329 | \n0.673 | \n0.327 | \n0.603 | \n
Inf mRNFL | \nTotal mRNFL | \n0.702 | \n0.410 | \n0.115 | \n0.275 | \n
Inf mRNFL | \nInf pRNFL | \n0.315 | \n0.204 | \n0.832 | \n0.601 | \n
Inf mRNFL | \nTotal pRNFL | \n0.125 | \n0.796 | \n0.884 | \n0.451 | \n
Total mRNFL | \nInf pRNFL | \n0.172 | \n0.050 | \n0.339 | \n|
Total mRNFL | \nTotal pRNFL | \n0.062 | \n0.296 | \n0.068 | \n0.132 | \n
Inf pRNFL | \nTotal pRNFL | \n0.558 | \n0.326 | \n0.694 | \n0.245 | \n
Comparison analysis in AUROC values in all groups.
We evaluated also sensitivity, specificity and cut-off values for the same RNFL parameters. In PPG group there are two parameters with highest and almost equal values of the sensitivity and specificity—Total mRNFL (sensitivity—0.83, specificity—0.77) and Inf mRNFL (sensitivity—0.82, specificity—0.79). These two parameters keep their high and close values of sensitivity also in the group of early perimetric glaucoma: Total mRNFL—0.93 и Inf mRNFL—0.90. The parameter with highest value of specificity in the same group is Total pRNFL—0.89, and after it are these parameters: Total mRNFL—0.81 and Inf mRNFL—0.79. In the PPG group with highest values is Total mRNFL (sensitivity—0.97 and specificity—0.95), and after it is Inf mRNFL (sensitivity—0.94 and specificity—0.85). It is observed very small differences in the values between investigated parameters, which decrease in advanced glaucoma group. With highest sensitivity (1.00) and specificity (1.00) in advanced glaucoma group is Total mRNFL, and after it is Inf pRNFL (1.00; 0.99) and Inf mRNFL (0.99, 0.99).
\nIn Table 5 can be seen the AUROC values of the macular parameters—Total mRNFL, Total GCL+ (ganglion cell layer/GCL + inner plexiform layer/IPL) and Total GCL++ (GCL + IPL + mRNFL) from protocol Glaucoma Analysis—Macula (see Figure 3, left). Lowest diagnostic accuracy for glaucoma in all investigated stages possesses the parameter—GCL+. The highest area under the curve has GCL++ (0.919, 0.932) in PPG group, Total mRNFL in the moderate glaucoma group (0.989), and the both parameters reach maximal possibilities for diagnosis in advanced glaucoma group (1.000). We also applied comparison analysis to find significance in diagnostic capabilities (AUROC values) between macular parameters. The results from this analysis could be seen in Table 6. Significance can be seen in the values between Total mRNFL and Total GCL+, and between Total GCL++ and Total GCL+. We did not find a difference between Total mRNFL and Total GCL++. This mean that the whole ganglion cell layer (consists of three sub-layers), which is presented by GCL++ parameter, has an equivalent diagnostic potential of those of Total mRNFL, which presents only one of the macular sub-layers (the most inner layer consists of the nerve fibers). The less accurate diagnostic potential from macular OCT parameters we found for GCL++. Therefore we exclude this parameter as an accurate in glaucoma diagnosis.
\nParameter | \nControls vs. | \n|||
---|---|---|---|---|
PPG | \nEarly glaucoma | \nModerate glaucoma | \nAdvanced glaucoma | \n|
AUROC | \nAUROC | \nAUROC | \nAUROC | \n|
Total mRNFL | \n0.879 | \n0.929 | \n0.989 | \n1.000 | \n
Total GCL+ | \n0.839 | \n0.858 | \n0.939 | \n0.993 | \n
Total GCL++ | \n0.919 | \n0.932 | \n0.987 | \n1.000 | \n
AUROC values of the GCL map parameters.
AUROC comparisons | \nControls vs. | \n||||
---|---|---|---|---|---|
PPG | \nEarly glaucoma | \nModerate glaucoma | \nAdvanced glaucoma | \n||
p | \np | \np | \np | \n||
Total mRNFL | \nTotal GCL+ | \n0.336 | \n|||
Total mRNFL | \nTotal GCL++ | \n0.268 | \n0.897 | \n0.819 | \n1.000 | \n
Total GCL+ | \nTotal GCL++ | \n
Comparison analysis between macular parameters’ AUROC values.
In the current research we found that the Topcon OCT parameter—Total mRNFL has the highest diagnostic accuracy in the very early stage of glaucoma, in which only structural changes could be seen (PPG). It is important to know its diagnostic possibilities compared with those of other OCT parameters, because it allows the clinicians to precise the early diagnosis, appropriate treatment and the most important for the patients—prevention of the vision loss. The results showed that this parameter also has the highest diagnostic possibilities in all perimetric glaucoma stages. These conclusions we made only after comparative analysis in diagnostic accuracy between all OCT parameters (peripapillary and macular) had been applied.
\nThere are not many researches, which investigate macular RNFL as a separate parameter not as a part of whole ganglion cell complex. Not enough data was collected about characteristics, correlations and diagnostic possibilities of mRNFL.
\nIn 2005 for the first time was created software algorithm for automated segmentation of retinal layers in Stratus OCT (OCT III). It helped authors differentiate four macular layers—macular nerve fiber layer (mNFL); inner retinal complex (IRC) consisting of ganglion cells, inner plexiform layer and inner nuclear layer; outer plexiform layer (OPL); outer retinal complex (ORC), consisting of outer nuclear layer, inner and outer photoreceptor segments. When the authors investigated diagnostic accuracy they found the highest values in mNFL+IRC (0.97), and lowest in OPL (0.56). Diagnostic accuracy of OPL and ORC was significantly lower from mNFL, IRC, mNFL+IRC and circumpapillary nerve fiber layer (cpNFL) (p ≤ 0.01). They found that AUROC values of IRC, mNFL+IRC and cpNFL were significantly higher from whole retinal thickness (p ≤ 0.049). It was not found significant differences between parameters with best diagnostic possibilities—mNFL, IRC, mNFL+IRC and cpNFL (p ≥ 0.15). The two parameters—ORC and OPL were found also to have almost permanent thickness in patients with glaucoma in comparison with healthy volunteers [28].
\nIn the beginning of the era “OCT diagnostics in glaucoma” was found that the whole retinal thickness decreases. Later with the initiation of spectral domain OCT (SD-OCT) in the clinical practice inner macular layers (mRNFL, GCL, IPL) were called a complex (ganglion cell complex—GCC), which consists of the bodies, dendrites and axons of the ganglion cells [29]. A self-evident fact is that GCC has significantly higher possibilities for glaucoma diagnosis than the thickness of the whole retina.
\nMwanza et al. investigated diagnostic accuracy of GCIPL (ganglion cell + inner plexiform layer), RNFL ONH parameters [30]. They found that GCIPL diagnostic possibilities are between 0.918 and 0.956, and there values are comparable with the best diagnostic parameters—RNFL (between 0.933 and 0.939) and ONH parameters (0.910 and 0.962) without statistically significant difference between them.
\nThere are two conceptions of ganglion cell loss in glaucoma. In the first—the dendrites die before the bodies, and the most resistant part of the cell of glaucoma damage are their axons. Therefore, it is reasonable to investigate the thickness of GCL + IPL separately from mRNFL. On the other hand IPL consists of the dendrites not only the ganglion cells but also the bipolar cells, and it is believed as more correctly to measure the thickness of mRNFL+GCL together.
\nPeripapillary RNFL is a proved glaucoma diagnostic parameter and also ganglion cell complex. Predominantly of the glaucoma comparisons in diagnostic accuracy are between pRNFL and GCC in different OCT devices.
\nThe current research investigate a new SD-OCT macular parameter—mRNFL and its diagnostic possibilities for different stages of POAG. It proves that mRNFL could be used in every day clinical practice of the ophthalmologist as independent parameter with very high diagnostic possibilities for early stages of glaucoma when only structural changes are visible.
\nNow we are working on creating of staging system based on Total mRNFL values (cut-off values) in each glaucoma group. It could give possibilities for the ophthalmologists to use the values of this parameter in everyday clinical practice to make diagnosis and follow-up of the glaucoma patients. This grading system will be the only of the OCT structural systems created up to date. Total mRNFL has the potential to be one of the best OCT diagnostic parameters and we as researchers must find how to use it in the diagnosis of very early glaucoma changes.
\nWe would like to acknowledge with much appreciation the crucial role of: Assoc. Prof. Todor Kundurjiev, PhD (Medical University of Sofia, Bulgaria, Department of Social Medicine and Health Management) who made the whole statistical analysis for the current research.
\nThe authors declare that there is no conflict of interest.
I would like to express my special appreciation and thanks to my teacher:
\nAssociate Professor Galina G. Dimitrova, MD, PhD.
\nYou have been a tremendous mentor for me. I would like to thank you for encouraging my work and for allowing me to grow as a research scientist. Your advice on both science and on my clinical practice has been invaluable. I appreciate this more than you know.
\nCancer is the dreadful disease in which cells divide uncontrollably and, at a later stage, begin attacking neighboring tissues. Hereditary mutations, toxin exposure, radiation exposure, alcohol usage, smoking, and radical lifestyle changes are all known to cause cancer. Early detection of cancer results in good therapy. The traditional diagnostic procedures of X-ray, CT-scan, and tissue biopsy are unable to detect it at an early stage, resulting in a delay in treatment that has resulted in the death of several people globally due to cancer [1, 2]. Substantial advances in cancer biology have resulted in the discovery of various biomolecules that are especially linked to cancer progression and development, and therefore referred to as “biomarkers.” Biomarkers are basically alterations which are cellular, biochemical, and molecular changes that can be used to identify or monitor a normal, abnormal, or just a biological process. They are utilized to test and evaluate pathogenic processes, normal biological processes, and the pharmacological response to a treatment intervention objectively. Biomarkers could be classified based on their chemical nature and functionality that can be identified using transcriptomics, metabolomics, genomics and proteomics (Figure 1) [3, 4].
Analysis of potential biomarkers using different integrated bioinformatics analysis assay platforms such as DNA based from FISH assay platform, RNA based biomarkers from micro arrays, protein based biomarkers from proteomic profiles and metabolites based on biomarkers from metabolomics profiles which led to screening of various kinds of cancer resulting in identification of potential candidate genes for prognostic therapeutic approach.
Usually, living cells have a finite life span, and their genome deoxy ribonucleic acid (DNA) transcribes into ribonucleic acid (RNA), which upon translation results in the creation of proteins that participate in numerous physiological and metabolic processes required by the body. Any change in these mechanisms, such as a mutation in DNA, causes disruption which leads to a dreadful disease namely, Cancer. The detection of mutations in DNA can be used to predict Cancer risk [5]. Consequently, measurement of RNA, protein, and metabolite expression levels can provide important information about illness progression and profiling. There are more than 200 types of cancer reported, however in this chapter, we gathered and presented information about various biomarkers associated with top 5 types of cancer in the world, which can be exploited in designing of sensitive and effective diagnostic technology for early detection of cancer. Basically, various types of biomarkers associated with different types of cancer and their identification using integrated bioinformatic analysis will be discussed. Besides, a brief insight on integrated bioinformatics analysis tools and databases have also been discussed.
Biomarkers have been generally known to play crucial role in the association with different cancer resulting in therapeutic aspects. These could be constructed with the help of advanced integrated bioinformatics analysis tools which could provide an ease to identify biomarkers which could be treated as potential candidates to treat diversities of Cancer. We have listed biomarkers associated with various types of cancer using integrated bioinformatics approaches in Table 1. The mechanistic insight regarding how the databases can be utilized to extract and identify various biomarkers associated with respective cancers have been depicted in Figure 2.
S. No. | Type of cancer | Biomarkers identified | Investigators | References |
---|---|---|---|---|
1 | Lung Cancer | TOP2A, CCNB1, CCNA2, UBE2C, KIF20A, and IL-6 | Ni et al., 2018 | [6] |
2 | CDC20, ECT2, KIF20A, MKI67, TPX2, and TYMS | Dai et al., 2020 | [7] | |
3 | DDX5, DDX11, DDX55 and DDX56 | Cui et al., 2021 | [8] | |
4 | NDC80, BUB1B, PLK1, CDC20, and MAD2L1 | Liao et al., 2019 | [9] | |
5 | UBE2T, UNF2, CDKN3, ANLN, CCNB2, and CKAP2L | Tu et al., 2019 | [10] | |
6 | UBE2C, AURKA, CCNA2, CDC20, CCNB1, TOP2A, ASPM, MAD2L1, and KIF11 | Liu et al., 2020 | [11] | |
7 | Gastric Cancer | CST2, AADAC, SERPINE1, COL8A1, SMPD3, ASPN, ITGBL1, MAP7D2, and PLEKHS1 | Liu et al., 2018 | [12] |
8 | FN1, COL1A1, INHBA, and CST1 | Wang et al., 2020 | [13] | |
9 | COL1A2 | Rong et al., 2018 | [14] | |
10 | LINC01018, LOC553137, MIR4435-2HG, and TTTY14 | Miao et al., 2017 | [15] | |
11 | UCA1, HOTTIP, and HMGA1P4 | Zang et al., 2019 | [16] | |
12 | Liver Cancer | PBK, ASPM, NDC80, AURKA, TPX2, KIF2C, and centromere protein F | Ji et al., 2020 | [17] |
13 | miR1055p, miR7675p, miR12665p, miR47465p, miR500a3p, miR11803p, and miR1395p | Shen et al., 2020 | [18] | |
14 | BUB1, CCNB2, CDC20, CDK1, KIF20A, KIF2C, RACGAP1 and CEP55 | Li et al., 2017 | [19] | |
15 | Breast Cancer | TXN, ANXA2, TPM4, LOXL2, TPRN, ADCY6, TUBA1C, and CMIP | Wang et al., 2019 | [20] |
16 | ADH1A, IGSF10, and the 14 microRNAs | Wu et al., 2021 | [21] | |
17 | TPX2, KIF2C, CDCA8, BUB1B, and CCNA2 | Cai et al., 2019 | [22] | |
18 | CDC45, PLK1, BUB1B, CDC20, AURKA and MAD2L1 | Wu et al., 2020 | [23] | |
19 | Colorectal Cancer | SLC4A4, NFE2L3, GLDN, PCOLCE2, TIMP1, CCL28, SCGB2A1, AXIN2, and MMP1 | Chen et al., 2019 | [24] |
20 | BLACAT1 | Dai et al., 2017 | [25] | |
21 | HMMR, PAICS, ETFDH, and SCG2 | Sun et al., 2021 | [26] | |
22 | hsa-miR-183-5p, hsa-miR-21-5p, hsa-miR-195-5p and hsa-miR-497-5p | Falzone et al., 2018 | [27] |
Biomarkers identified by using integrated bioinformatics tools, associated with various types of cancer such as lung cancer, gastric cancer, colorectal cancer.
The schematic representation of extraction of datasets from the GEO database then the identification of DEGs followed by its functional analysis and subsequent qPCR validation leading to identification of small molecule known as biomarker for treating Cancer.
Lung cancer is the most common cancer-related death around the globe. Despite great attempts to enhance treatment approaches in previous decades, the clinical outcome of traditional therapies such as surgery, radiation, and chemotherapy remains poor when compared to other major forms of cancer such as colon, prostate, and breast cancers. The challenges in making an early-stage diagnosis of lung cancer and the high recurrence rate after curative treatments are the main reasons for the lack of improvement in prognosis [28]. To improve the clinical result of lung cancer treatments, it is critical to identify and validate diagnostic and prognostic biomarkers. Therefore, here in this section of chapter we have reviewed studies led by certain researchers for identification of the lung cancer biomarkers using integrated bioinformatics analysis. There are mainly 2 types of the lung cancer. In 80–85% cases, the type of lung cancer is non-small cell lung cancer (NSCLC). The main subtypes of which are adenocarcinoma, squamous cell carcinoma, and large cell carcinoma. These subtypes generally begin from different types of the lung cells that are grouped together as NSCLC and their treatment and prognoses are almost similar. The other type is small cell lung cancer (SCLC) and around 10–15% of all lung cancers are SCLC and it is sometimes called oat cell cancer. SCLC grows and spread faster than NSCLC.
In a study by Ni et al., four GEO datasets GSE18842, GSE19804, GSE43458, and GSE62113, were extracted form Gene Expression Omnibus (GEO) database into which the limma package was used to assess differentially expressed genes (DEGs) between NSCLC and normal samples, and the RobustRankAggreg (RRA) programme was used to undertake gene integration. Furthermore, they established the protein–protein interaction (PPI) network of these DEGs using the Search Tool for the Retrieval of Interacting Genes database (STRING), Cytoscape, and Molecular Complex Detection (MCODE). Funrich (http://www.funrich.org)and OmicShare (https://www.omicshare.com/tools/)were also conducted to ensure functional enrichment and pathway enrichment analysis for DEGs. Besides this, they used the gene Expression Profiling Interactive Analysis (GEPIA) and Kaplan Meier-plotter (KM) online datasets to analyze the expressions and prognostic values of top genes. Hence, it led to the identification of a total of 249 DEGs including 113 upregulated and 136 downregulated after gene integration. Followed by this, they established a PPI network with 166 nodes and 1784 protein pairings resulting in TOP2A, CCNB1, CCNA2, UBE2C, KIF20A, and IL-6 to be considered as possible important genes, whereas they further added, the mitotic cell cycle pathway to play a crucial role in NSCLC advancement resulting in its employment as a novel biomarker for NSCLC diagnosis and to guide synthesis medication [6].
In another study by Dai et al., 6 key biomarkers associated with non- small cell lung cancer in which GEO2R were analyzed to examine three microarray datasets from the Gene Expression Omnibus collection along with the enrichment analysis which was performed using Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes. Further, the String database, Cytoscape, and the MCODE plug-in were then used to build a PPI network and screen hub genes using the String database, Cytoscape, and the MCODE plug-in. Kaplan–Meier curves were used to examine overall and disease-free survival of hub genes, as well as the association between target gene expression patterns and tumor grades. To verify enrichment pathways and diagnostic effectiveness of hub genes, researchers performed gene set enrichment analysis and receiver operating characteristic curves. A total of 293 differentially expressed genes were discovered, with cell cycle, ECM–receptor interaction, and malaria being the most prevalent. The PPI network identified 36 hub genes, six of which were reported to have important roles in NSCLC (non- small cell lung cancer) carcinogenesis: CDC20, ECT2, KIF20A, MKI67, TPX2, and TYMS. The target genes discovered can be employed as potential biomarkers to identify and diagnose non- small cell lung cancer as per their investigations [7].
Similarly, in another study by Cui et al., they used integrated bioinformatic analysis of multivariate large-scale databases to assess the potential of DEAD/H box helicases as prognostic indicators and therapeutic targets in lung cancer. They were able to discover four biomarkers with the most significant changes after analyzing the survival and differential expression of these helicases. The unfavorable prognostic factors DDX11, DDX55, and DDX56, as well as the good prognosis factor DDX5, were discovered. MYC signaling is adversely linked with DDX5 gene expression, but favorably associated with DDX11, DDX55, and DDX56 gene expression, according to pathway enrichment analysis led by them. Low mutation levels of TP53 and MUC16, the two most frequently mutated genes in lung cancer, are related with high expression levels of the DDX5 gene. High levels of DDX11, DDX55, and DDX56 gene expression, on the other hand, were linked to high levels of TP53 and MUC16 mutation. The levels of DDX5 gene expression in tumor-infiltrated CD8 + T and B cells are positively correlated, but the other three DEAD box helicases are negatively correlated. Furthermore, while each DDX has a unique miRNA signature, the DDX5-associated miRNA profile is distinct from the miRNA profiles of DDX11, DDX55, and DDX56. The discovery of these four DDX helicases as biomarkers could be considered useful for lung cancer prognostication and targeted treatment development [8].
In another study by Liao et al., they have identified candidate genes associated with the pathogenesis of small cell lung cancer analyzed using integrated bioinformatics tools. GSE60052, GSE43346, GSE15240, and GSE6044 were the four datasets that they downloaded from the Gene Expression Omnibus. R software was used to examine the differentially expressed genes (DEGs) between the SCLC and normal samples. For each dataset, the limma software was utilized. The DEGs from the four datasets were combined using the RobustRankAggreg package. FunRich software and R software were used to conduct functional and route enrichment analyses using the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases, accordingly. The DEGs’ protein–protein interaction (PPI) network was also built using the STRING database and the Cytoscape software. Molecular Complex Detection in Cytoscape software was used to find hub genes and important modules. Ultimately, the Oncomine online database was used to assess the expression values of hub genes. Following the integration of the four datasets, 412 DEGs were discovered, comprising 146 upregulated genes and 266 downregulated genes. The increased DEGs were mostly involved in cell division, cell cycle, and microtubule binding. The complement and coagulation cascades, the cytokine-mediated signaling pathway, and protein binding were all heavily represented among the downregulated DEGs. Based on a subset of the PPI network, eight hub genes and one major module connected to the cell cycle pathway were discovered. Eventually, in comparison to normal tissue, five hub genes were shown to be substantially expressed in SCLC tissue. The cell cycle route may be the one that is most closely linked to SCLC pathophysiology. As a result, follow-up studies in the diagnosis and therapy of SCLC should focus on NDC80, BUB1B, PLK1, CDC20, and MAD2L1 [9].
In another similar study by Tu et al., GEO2R was used to search the mRNA microarray datasets GSE19188, GSE33532, and GSE44077 for differentially expressed genes (DEGs). The DEGs were analyzed for functional and pathway enrichment using the DAVID database. STRING was used to create a protein–protein interaction (PPI) network, which was then displayed in Cytoscape. MCODE was used to analyze the PPI network’s modules. The Kaplan Meier-plotter was used to analyze the overall survival (OS) of genes from MCODE. Total of 221 DEGs were found, with words linked to cell division, cell proliferation, and signal transduction being the most abundant. A PPI network with 221 nodes and 739 edges was created. The PPI network revealed a substantial module containing 27 genes. UBE2T, UNF2, CDKN3, ANLN, CCNB2, and CKAP2L all have high expression levels and have been linked to a poor prognosis in NSCLC patients. Protein binding, ATP binding, cell cycle, and the p53 signaling pathway were among the enriched functions and pathways. DEGs in non- small cell lung cancer (NSCLC) have the potential to be useful targets for diagnosing and treating the disease [10].
In another study by Liu et al., in this prospective investigation, which included 46 tumors and 45 controls, the gene expression profile GSE18842 was acquired from the Gene Expression Omnibus database. They used functional enrichment analysis and KEGG analysis using upregulated differentially expressed genes (uDEGs) and downregulated differentially expressed genes (dDEGs), respectively, after screening differentially expressed genes (DEGs). The STRING database was used to create protein–protein interaction (PPI) networks between DEGs and their corresponding coding protein complexes, which were then examined using Cytoscape. The Kaplan–Meier approach was used to confirm the survival of hub genes. In the TCGA database, the gene expression level heat map of hub genes between NSCLC and neighboring lung tissues was plotted using the GEPIA webserver. After gene integration, they found 368 DEGs (168 uDEGs and 200 dDEGs) in NSCLC samples compared to control samples. They built a PPI network for the DEGs with 249 nodes and 1472 protein pairings on the edges. Survival study confirmed that ten undefined hub genes with the highest connectivity degree (CDK1, UBE2C, AURKA, CCNA2, CDC20, CCNB1, TOP2A, ASPM, MAD2L1, and KIF11) were related with lower overall survival in NSCLC. The GEPIA web tool was used to verify the expression dependability of hub genes. The findings suggested that UBE2C, AURKA, CCNA2, CDC20, CCNB1, TOP2A, ASPM, MAD2L1, and KIF11 are inherent critical biomarkers for diagnosis and prognosis, and that the mitotic cell cycle pathway is a likely signaling pathway contributing to NSCLC progression, according to KEGG analysis. Such genes could be useful diagnostic biomarkers, as well as a new strategy to designing targeted NSCLC treatments [11].
Despite a substantial drop in incidence and death in North America and most Western European countries in recent decades, gastric cancer (GC) remains the fifth most prevalent malignancy worldwide and poses a serious medical burden, particularly in Eastern Asia [29, 30]. The fact that most patients are discovered at an advanced stage, even with metastatic illnesses, and thus miss out on the potential for a curative resection, accounts for the poor 5-year survival in GC [31, 32]. Substantial progress has been made in comprehending the epidemiology, pathophysiology, and molecular mechanisms of GC, as well as in implementing new therapy alternatives like as targeted and immune-based therapies, not all patients react to molecularly targeted medications developed for specific biomarkers [32, 33]. Hence, due to molecular complexity, poor prognosis, and significant reoccurrence of GC, new diagnostic and prognostic biomarkers are urgently needed [34, 35]. Microarray and high-throughput sequencing technologies have advanced in recent years, allowing researchers to decipher important genetic or epigenetic changes in carcinogenesis and discover promising biomarkers for cancer diagnosis, treatment, and prognosis [36]. Nevertheless, integrated bioinformatics methods have been used in cancer research to overcome limited or inconsistent results due to the use of different technology platforms or a small sample size, and a large range of valuable biological information has been revealed [37, 38, 39].
Hence, here we have reviewed a few studies to ensure the role of biomarker identification associated to gastric cancer using integrated bioinformatics analysis tools. In a study by Liu et al., they have considered TOP2A, COL1A1, COL1A2, NDC80, COL3A1, CDKN3, CEP55, TPX2, and TIMP1 which are nine hub genes that may be linked to the etiology of GC. Hence, CST2, AADAC, SERPINE1, COL8A1, SMPD3, ASPN, ITGBL1, MAP7D2, and PLEKHS1 were used to construct a prognostic gene signature that performed well in predicting overall survival. An integrated analysis of several gene expression profile datasets was used by them to find differentially expressed genes between GC and normal gastric tissue samples. Furthermore, protein–protein interaction network and Cox proportional hazards model studies were used to identify key genes related to the pathophysiology and prognosis of GC resulting in their constructed gene signature to be considered as a potential candidate for the biomarker to facilitate the molecular targeting therapy of GC [12].
In a study by Wang et al., they discovered promising biomarkers that could be used to diagnose GC patients. Four Gene Expression Omnibus (GEO) datasets were obtained and examined for differentially expressed genes to look for possible treatment targets for GC (DEGs). The function and pathway enrichment of the discovered DEGs were then investigated using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. A network of protein–protein interactions (PPI) was created. The degree of connection of proteins in the PPI network was calculated using the CytoHubba plugin of Cytoscape, and the two genes with the highest degree of connectivity were chosen for further investigation. The two DEGs with the highest and lowest log Fold Change values were also chosen. Oncomine and the KaplanMeier plotter platform were used to investigate these six important genes further. A total of 99 genes that were upregulated and 172 genes that were downregulated across all four GEO datasets were examined. The Biological Process phrases ‘extracellular matrix organization,’ ‘collagen catabolic process,’ and ‘cell adhesion’ were primarily enriched in the DEGs. The categories ‘ECMreceptor interaction,’ ‘protein digestion and absorption,’ and ‘focal adhesion’ were considerably enriched in these three KEGG pathways. According to Oncomine, ATP4A and ATP4B expression were downregulated in GC, while all other genes were increased. Upregulated expression of the identified important genes was substantially associated with worse overall survival of GC patients, according to the KaplanMeier plotter platform. The current findings imply that FN1, COL1A1, INHBA, and CST1 could be used as gastric cancer biomarkers and treatment targets. Additional research is needed to determine the role of ATP4A and ATP4B in the treatment of gastric cancer [13].
In another study by Rong et al., their research outlines an integrated bioinformatics approach to identifying molecular biomarkers for stomach cancer in cancer tissues of patients. In large gastric cancer cohorts, they reported distinct expression genes from Gene Expression Ominus (GEO). Their findings found that 433 genes in human stomach cancer have significantly distinct expression patterns. Bioinformatic studies and co-expression network design were used to confirm the different gene expression profiles in gastric cancer. They identified collagen type I alpha 2 (COL1A2), which encodes the pro-alpha2 chain of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain, as the key gene in a 37-gene network that modulates cell motility by interacting with the cytoskeleton, based on the co-expression network and top-ranked genes. Immunohistochemistry on human gastric cancer tissue was also used to investigate the predictive function of COL1A2. When compared to normal gastric tissues, COL1A2 was substantially expressed in human gastric cancer. The level of COL1A2 expression was found to be substantially related to histological type and lymph node status after statistical analysis. There were no links found between COL1A2 expression and age, lymph node count, tumor size, or clinical stage. Finally, the unique bioinformatics used in this study led to the discovery of improved diagnostic biomarkers for human stomach cancer, which could aid future research into the crucial change that occurs during the disease’s course [14].
In another study, the goal of their research is to find an lncRNA-related signature that can be used to assess the overall survival of 379 GC patients from The Cancer Genome Atlas (TCGA) database. The univariate and multivariate Cox proportional hazards regression models were used to assess the correlations between survival outcome and the expression of lncRNAs. Overall survival was found to be substantially linked with four lncRNAs (LINC01018, LOC553137, MIR4435-2HG, and TTTY14). These four lncRNAs were combined to form a prognostic signature. There was a strong favorable link between overall survival and GC patients with low-risk scores (P = 0.001). Subsequent research found that the predictive usefulness of this four-lncRNA pattern was unaffected by clinical characteristics. These four lncRNAs were linked to many tumor molecular pathways, according to gene set enrichment analysis. Based on bioinformatics analysis, their research suggests that this unique lncRNA expression pattern could be a helpful diagnostic of prognosis for GC patients [15].
The researchers wanted to see if there were any long noncoding RNAs (lncRNAs) that were linked to the pathophysiology and prognosis of GC. The Gene Expression Omnibus (GEO) database was used to retrieve raw noncoding RNA microarray data (GSE53137, GSE70880, and GSE99417). After gene reannotation and batch normalization, an integrated analysis of various gene expression profiles was used to screen for differentially expressed genes between GC and neighboring normal stomach tissue samples. The Cancer Genome Atlas (TCGA) database validated the presence of differentially expressed genes. To identify hub lncRNAs and explore possible biomarkers related to GC diagnosis and prognosis, researchers used a competitive endogenous RNA (ceRNA) network, Gene Ontology term, and Kyoto Encyclopedia of Genes and Genomes pathway, as well as survival analysis. After intersections of differential genes between the GEO and TCGA databases, a total of 246 integrated differential genes were identified, including 15 lncRNAs and 241 messenger RNAs (mRNAs). Three lncRNAs (UCA1, HOTTIP, and HMGA1P4), 26 microRNAs (miRNAs), and 72 mRNAs make up the ceRNA network. Three lncRNAs controlled the cell cycle and cellular senescence, according to functional analyses. The survival rate of HMGA1P4 was statistically connected to the total survival rate, according to a survival analysis. They discovered that HMGA1P4, a miR-301b/miR-508 target, regulates CCNA2 in the GC and is implicated in cell cycle and senescence. Ultimately, three lncRNAs’ expression levels were shown to be elevated in GC tissues. As a result, three lncRNAs, UCA1, HOTTIP, and HMGA1P4, may play a role in GC development, and their possible functions may be linked to GC prognosis [16].
Liver cancer is among the most frequent malignancies in the world, and it is the second largest cause of cancer death [40, 41]. Due to advances in detection and therapy, people with liver cancer still have a terrible prognosis. Most patients are already in severe stages of symptoms and miss the opportunity to undertake radical resection due to the lack of distinct clinical signs in the early stages. As a result, understanding the pathophysiology of liver cancer aids in early detection, treatment selection, scheduling of follow-up appointments, and prognosis evaluation, all of which can help patients with liver cancer live longer [42]. MicroRNAs (miRNAs) are improperly expressed in a range of tumors and are linked to the pathogenesis of cancers, including liver cancer, according to growing evidence. As tumor suppressor genes or oncogenes, miRNAs play a role in the development of liver cancer. As a result, more research into miRNA expression patterns and consequences could lead to the discovery of new diagnostic or therapeutic targets for liver cancer. Hence, here in this subsection of this chapter we have reviewed certain researches which provide a potential aspect toward identification of biomarkers associated with cancer in relevance to liver utilizing integrated bioinformatics analysis.
Hepatitis B virus (HBV) infection has long been known as a major risk factor for hepatocellular carcinoma (HCC), accounting for at least half of all HCC cases worldwide. Yet, the underlying molecular mechanism of HBV-associated HCC is still unknown. Hence, in an investigation led by Ji et al., they retrieved three microarray datasets from the Gene Expression Omnibus (GEO) collection, including 170 tumoral samples and 181 adjacent normal tissues from the liver of patients with HBV-related HCC which were subjected to integrated analysis of differentially expressed genes (DEGs). Following that, the protein–protein interaction network (PPI) and function and pathway enrichment analyses were carried out. The expression profiles and survival analyses of the ten hub genes selected from the PPI network were carried out. Overall, 329 DEGs were discovered in which 67 were upregulated and 262 were downregulated. PDZ-binding kinase (PBK), abnormal spindle microtubule assembly (ASPM), nuclear division cycle 80 (NDC80), aurora kinase A (AURKA), targeting protein for xenopus kinesin-like protein 2 (TPX2), kinesin family member 2C (KIF2C), and centromere protein F were among the ten DEGs with the highest degree of connectivity (CENPF). Overexpression levels of KIF2C and TPX2 were linked to both poor overall survival and relapse-free survival in a Kaplan–Meier study. Therefore, the hub genes identified in this investigation could be useful in the diagnosis, prognosis, and treatment of HBV-related HCC. Furthermore, their research identifies a number of important biological components (e.g., extracellular exosomes) and signaling pathways that are involved in the progression of HCC caused by HBV, providing a more thorough understanding of the mechanisms underlying HBV-related HCC [17].
In another study by Shen et al., they created nine co-expression modules and discovered that in liver cancer, miR1055p, miR7675p, miR12665p, miR47465p, miR500a3p, miR11803p, and miR1395p were differentially expressed. These miRNAs were found to have a strong link to the prognosis of patients with liver cancer. MiR1055p and miR1395p may be considered separate prognostic variables among them. As a result, seven miRNAs could be used as predictive indicators in the case of liver cancer [18].
In another study by Li et al., The GSE19665, GSE33006, and GSE41804 microarray datasets were obtained from the Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) were found and function enrichment analyses were carried out. STRING and Cytoscape were used to create the protein–protein interaction network (PPI) and perform module analysis. There were a total of 273 DEGs found, with 189 downregulated genes and 84 upregulated genes. Protein activation, complement activation, carbohydrate binding, complement and coagulation cascades, mitotic cell cycle, and oocyte meiosis are among the DEGs’ enhanced activities and pathways. A biological process study found that these genes were primarily abundant in cell division, cell cycle, and nuclear division. BUB1, CDC20, KIF20A, RACGAP1 and CEP55 were found to be involved in the carcinogenesis, invasion, and recurrence of HCC in a survival analysis. Finally, the DEGs and hub genes discovered in this work contribute to our understanding of the molecular pathways underlying HCC carcinogenesis and development, as well as providing candidate targets for HCC diagnosis and treatment [19].
Breast cancer is becoming more common over the world, and it is now considered a serious disease among women. Asia has recently emerged as a high-risk location for breast cancer, ranking first among female malignant tumors [43, 44]. Breast cancer therapy has improved recently as a result of constant efforts and advances in contemporary medicine, and the death rate of breast cancer has decreased dramatically. Recurrence and metastasis of breast cancer, on the other hand, have remained unaddressed and have become the most difficult clinical difficulties [43, 45]. To better understand the functions of tumor-related genes and the roles of tumor cell signaling pathways, researchers are turning to genetic studies. Together bioinformatics and system biology are strong multidisciplinary topics that combine biological information collecting, storage, processing, and distribution, summarize life sciences and computer science, and collect and analyze genetic data [46, 47]. Hence, here in this chapter we have reviewed a few studies led by researchers to identify most prevalent biomarkers associated with breast cancer utilizing integrated bioinformatics approaches.
In an investigation by Wang et al. they have analyzed gene expression profiles of GSE48213 using Gene Expression Omnibus database. Further, validation was done using RNA-seq data and clinical information on breast cancer from The Cancer Genome Atlas. In their study, they identified the gene co- expression network which revealed four modules, one of which was found to be strongly linked with patient survival time. They found that the black module which was found to be basal, was made up of 28 genes; the dark red module which was found to be claudin-low, was made up of 18 genes; the brown module which was found to be luminal, was made up of nine genes; and the midnight blue module was made up of seven genes which was investigated to be nonmalignant. Due to a considerable difference in survival time between the two groups, these modules were clustered into two groups. Hence, TXN and ANXA2 in the nonmalignant module, TPM4 and LOXL2 in the luminal module, TPRN and ADCY6 in the claudin-low module, and TUBA1C and CMIP in the basal module were identified by them as the genes with the highest betweenness, implying that they play a central role in information transfer in the network. Therefore, TXN, ANXA2, TPM4, LOXL2, TPRN, ADCY6, TUBA1C, and CMIP are eight hub genes that have been identified and validated by them as being linked to breast cancer progression and poor prognosis to be considered [20].
In another study by Wu et al., Differentially expressed genes (DEGs) in breast cancer were discovered using three data sets from the GEO database. The functional roles of the DEGs were determined using Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes pathway studies. They also used the Gene Expression Profiling Interactive Analysis (GEPIA), Oncomine, Human Protein Atlas, and Kaplan Meier plotter tool databases to look at the translational and protein expression levels, as well as survival statistics, of DEGs in patients with breast cancer. Using miRWalk and TargetScan, the corresponding change in the expression level of microRNAs in DEGs was predicted, and the expression profiles were evaluated using OncomiR. Finally, RT-qPCR was used to confirm the expression of new DEGs in Chinese breast cancer tissues. ADH1A, IGSF10, and the 14 microRNAs have all been identified as promising new biomarkers for breast cancer diagnosis, therapy, and prognosis [21].
In another study by Cai et al., the Gene Expression Omnibus (GEO) database was used to obtain GSE102484 gene expression profiles. The most potent gene modules related with the metastatic risk of breast cancer were found using weighted gene co-expression network analysis (WGCNA), which yielded a total of 12 modules. 21 network hub genes (MM > 0.90) were kept for further analysis in the most significant module (R2 = 0.68). The biomarkers with the greatest interactions in gene modules were then investigated further using protein–protein interaction (PPI) networks. Five hub genes (TPX2, KIF2C, CDCA8, BUB1B, and CCNA2) were identified as important genes associated with breast cancer progression by the PPI networks. Furthermore, using data from The Cancer Genome Atlas (TCGA) and the Kaplan–Meier (KM) Plotter, the predictive value and differential expression of these genes were confirmed. The mRNA expression levels of these five hub genes have excellent diagnostic value for breast cancer and surrounding tissues, according to a Receiver Operating Characteristic (ROC) curve study. Furthermore, KM Plotter revealed that these five hub genes were substantially related with lower distant metastasis-free survival (DMFS) in the patient group. Five hub genes (TPX2, KIF2C, CDCA8, BUB1B, and CCNA2) linked to the likelihood of distant metastasis were extracted for future study and could be employed as biomarkers to predict breast cancer distant metastasis [22].
In another study by Wu et al., there were a total of 215 DEGs found, with 105 upregulated genes and 110 downregulated genes. The enriched keywords and pathways were primarily linked to cell cycle, proliferation, drug metabolism, and oncogenesis, according to GO and KEGG analyses. Cell Division Cycle 45 (CDC45), Polo Like Kinase 1 (PLK1), BUB1 Mitotic Checkpoint Serine/Threonine Kinase B (BUB1B), Cell Division Cycle 20 (CDC20), Aurora Kinase A (AURKA), and Mitotic Arrest Deficient 2 Like 1 were identified as hub genes from the PPI network (MAD2L1). These hub genes’ resilience was confirmed by survival analysis and expression validation tests [23].
CRC (colorectal cancer) is one of the top causes of death among cancer patients around the world. Older age, male sex, lifestyle, inflammatory bowel illness, and a previous personal history of CRC are all risk factors for the disease. A positive family history is also substantially linked to a higher lifetime relative risk of CRC diagnosis. CRC, on the other hand, is an indolent disease in its early stages, becoming symptomatic only when it evolves to more advanced stages. Numerous attempts have been made to develop adequate screening technologies, but they remain intrusive even now, resulting in reduced attainment rates among large community [48]. Recent breakthroughs in our understanding of the molecular underpinnings and cellular mechanisms of CRC have resulted in the widespread use of particular molecular diagnostics in clinical practice. The patient’s risk is stratified and therapy is decided based on the test results. Conversely, current research into biomarkers associated with colorectal cancer could usher in a new age in diagnosis, risk prediction, and treatment selection. Here, we have reviewed a few investigations led to ensure its attainment using integrated bioinformatics analysis [49].
In an investigation led by Chen et al., they analyzed 207 common DEGs in colorectal cancer using the integrated GEO and TCGA databases into which they constructed a PPI network consists of 70 nodes and 170 edges and identified 10 top hub genes. A prognostic gene signature which includes SLC4A4, NFE2L3, GLDN, PCOLCE2, TIMP1, CCL28, SCGB2A1, AXIN2, and MMP1 was constructed by them which revealed overall survival in patients suffering from CRC. Hence, it could be considered as a good potential candidate for further treatments [24].
In a study by Dai et al., they discovered nine differentially expressed lncRNAs and their putative mRNA targets using integrated data mining. They evaluated key pathways and GO words that are associated to the up-regulated and down-regulated transcripts, respectively, after a series of bioinformatics investigations. Meanwhile, qRT-PCR was used to validate the nine lncRNAs in 30 matched tissues and cell lines, and the results were largely compatible with the microarray data. They also looked for nine lncRNAs in the blood of 30 CRC patients with tissue matching, 30 non-cancer patients, and 30 healthy people. Finally, they discovered that BLACAT1 was important for CRC diagnosis. Between CRC patients and healthy controls, the area under the curve (AUC), sensitivity, and specificity were 0.858 (95% CI: 0.765–0.951), 83.3%, and 76.7%, respectively. Furthermore, BLACAT1 exhibited a particular utility in distinguishing CRC from non-cancer disorders. The findings suggest that significantly elevated lncRNAs as well as associated potential target transcripts could be used as therapeutic targets in CRC patients. Conversely, the lncRNA BLACAT1 could be a new supplemental biomarker for CRC detection [25].
In another study by Sun et al., The Gene Expression Omnibus (GEO) mRNA microarray datasets GSE113513, GSE21510, GSE44076, and GSE32323 were collected and processed with bioinformatics to discover hub genes in CRC development. The GEO2R tool was used to look for differentially expressed genes (DEGs). The DAVID database was used to conduct gene ontology (GO) and KEGG studies. To build a protein–protein interaction (PPI) network and identify essential modules and hub genes, researchers employed the STRING database and Cytoscape software. The DEGs’ survival studies were done using the GEPIA database. Potential medications were screened using the Connectivity Map database. There were a total of 865 DEGs found, with 374 upregulated and 491 downregulated genes. These DEGs were mostly linked to metabolic pathways, cancer pathways, cell cycle pathways, and so on. With 863 nodes and 5817 edges, the PPI network was discovered. HMMR, PAICS, ETFDH, and SCG2 were found to be strongly linked with overall survival of CRC patients in a survival analysis. Blebbistatin and sulconazole have also been discovered as potential treatments [26].
Falzone et al. used the mirDIP gene target analysis in a sample of 19 differentially expressed miRNAs to determine the interaction between miRNAs and the most changed genes in CRC. DIANA-mirPath prediction analysis was used to identify miRNAs that can activate or inhibit genes and pathways involved in colorectal cancer development. As a whole, these studies found that the up-regulated hsa-miR-183-5p and hsa-miR-21-5p, as well as the down-regulated hsa-miR-195-5p and hsa-miR-497-5p, were linked to colorectal cancer development via interactions with the Mismatch Repair pathway and the Wnt, RAS, MAPK, PI3K, TGF-, and p53 signaling pathways [27].
Various integrated bioinformatics databases have been utilized for the identification of prognostic biomarkers in the treatment of various kinds of cancer. Some of which have been enlisted in Table 2 along with database links. The biomarkers associated with different types of Cancers identified with the help of integrated bioinformatics tools depicted in Figure 3.
S. No. | Name of database | Link/URL |
---|---|---|
1 | Gene Expression Omnibus (GEO) | http://www.ncbi.nlm.nih.gov/geo/ |
2 | GEO2R | http://www.ncbi.nlm.nih.gov/geo/geo2r/ |
3 | DAVID | http://david.abcc.ncifcrf.gov/ |
4 | STRING | http://www.bork.embl-heidelberg.de/STRING/ |
5 | Cytoscape | http://www.cytoscape.org/ |
6 | GEPIA | http://gepia2021.cancer-pku.cn/ |
7 | TGCA | https://tcga-data.nci.nih.gov/tcga/ |
8 | Kaplan–Meier (KM) Plotter | http://kmplot.com/analysis/ |
9 | DIANA-mirPath | http://www.microrna.gr/miRPathv3 |
10 | mirDIP | http://ophid.utoronto.ca/mirDIP |
11 | GOplot | http://cran.r-project.org/web/packages/GOplot |
12 | clueGO | http://apps.cytoscape.org/apps/cluego |
13 | MCODE | http://baderlab.org/Software/MCODE |
14 | GTEx | https://gtexportal.org |
15 | Oncomine | http://www.oncomine.org/resource/login.html |
16 | Human Protein Atlas | www.proteinatlas.org |
17 | miRWalk | http://mirwalk.uni-hd.de/ |
18 | TargetScan | www.targetscan.org |
19 | OncomiR | http://www.oncomir.org/oncomir/search_target_miR.html |
List of databases used for data mining.
Mechanistic insight of extraction, construction and identification of biomarkers associated with different kinds of cancers with the help of integrated bioinformatics tools.
The microarray data collection is done using the GEO database which refers to Gene Expression Omnibus. It could be easily accessed via online medium using http://www.ncbi.nlm.nih.gov/geo/link. The GEO database is basically being used to obtain high-throughput gene expression profiles of PTC (Papillary thyroid carcinoma) and normal thyroid tissues. Independent datasets are chosen, and they are all based on the specified platforms, including the relevant tissues. As per our review of various studies which are aforementioned in this chapter, various microarray datasets have been collected using the GEO database and then processed with bioinformatics to discover hub genes. Several new technologies have emerged for the analysis of gene expression and for the identification of cancer biomarkers. One such technology is RNASeq technology which is nowadays considered to be the most up to date technology to analyze gene expression. Into this technology, with the use of NGS (Next generation genome sequencer) the gene expression profile analysis carried out. The first stage in the process is to convert the population of RNA to be sequenced into complementary DNA (cDNA) fragments which is present in biological sample (a cDNA library). This is accomplished using reverse transcription, allowing the RNA to be used in an NGS procedure. After that, the cDNA is fragmented, and adapters are attached to each fragment’s end. The functional elements present on adopters which allowed sequencing. The cDNA library is evaluated by NGS after amplification, size selection, clean-up, and quality verification, yielding short sequences that correspond to all or part of the fragment from which it was formed. The extent to which the library is sequenced is determined by the intended use of the output data. Sequencing can be done in one of two ways: single-end or paired-end. Single-read sequencing is a less expensive and faster method of sequencing cDNA fragments from only one end (approximately 1% of the cost of Sanger sequencing). While paired-end approaches are more expensive since they sequence from both ends, but they provide advantages in post-sequencing data reconstruction. After completing the RNA sequencing technology workflow, the data can be matched to a reference genome if one is available, or built from scratch to provide an RNA sequence map that encompasses the transcriptome. A bioinformatics workflow is developed to discover various alternative biomarkers via LC- MS/MS technique (liquid chromatography coupled tandem mass spectrometry). Further, open Mass spectrometry Search Algorithm is used against the customized alternative splicing database along with the preferred cancer plasma proteome for the identification of respective biomarker [50, 51].
The GEO2R program which could be easily accessed via http://www.ncbi.nlm.nih.gov/geo/geo2r/link, is used for the detection of these differentially expressed genes which are known as DEGs. Further, R package Limma is been utilized to screen out these DEGs.
Followed by the screening of DEGs, the enrichment analysis using GO and KEGG pathway is performed using the database for Annotation, Visualization and Integrated Discovery, commonly known as DAVID database (http://david.abcc.ncifcrf.gov/). This process includes biological processes, cellular components, molecular function and KEGG pathway analysis. Further, the GOplot package of R could be used to display the results of analysis and the pathway analysis results can also be analyzed using the clueGO plug-ins of cytoscape software 3.7.2. [52].
After the enrichment analysis, the PPI network is being built upon using the STRING (http://www.bork.embl-heidelberg.de/STRING/) database which refers to Search Tool for the Retrieval of Interacting Genes/Proteins, to uncover DEG associations based on minimum prescribed interaction scores. Followed by this, using the Cytoscape (http://www.cytoscape.org/) database, the PPI network is then analyzed and visualized. Additionally, MCODE is also one such bioinformatics tool utilized to screen the PPI network’s main module.
At last. The Cancer Genome Atlas (https://tcga-data.nci.nih.gov/tcga/), was utilized to examine the association between important gene expression and survival of patients with PTC (Papillary thyroid carcinoma). RNA expression data from hundreds of samples from the TCGA and GTEx projects was analyzed using the Gene Expression Profiling Interactive Analysis tool (GEPIA) (http://gepia2021.cancer-pku.cn/). Additionally Oncomine, Human Protein Atlas, and Kaplan Meier plotter tool databases could also be used to look at the translational and protein expression levels, as well as survival statistics, of DEGs. Apart from this, miRWalk and TargetScan, were used to predict the corresponding change in the expression level of microRNAs in DEGs and the expression profiles were evaluated using OncomiR. Finally, RT-qPCR has been used to confirm the expression of new DEGs. Hence, the constructed biomarkers could be treated as potential candidates for various kinds of Cancers.
The development of biomarkers for early detection cancer screening and therapy monitoring has biological as well as financial hurdles. The majority of existing cancer detection tools only detect late stage or fully grown cancer, not premalignant or early abnormalities that can be resected and treated. Despite the fact that a screening test may detect cancer just at preclinical stage, it is not suitable for follow-up, and hence may miss micro metastases, limiting the benefits of early identification and treatment [53]. Additional barrier to the development of cancer biomarkers is the fact that cancer is a diverse illness, with several biologically distinct phenotypes that respond differently to treatments. Between cells of a single macroscopic tumor, the nature of its heterogeneity can be found. Biomarker development may be hampered by this variability. As a result, developing biomarkers using genomic and proteomic methods could help to solve the variability challenges [3]. An even more issue is that pre-neoplastic lesions are far more common than aggressive malignancies in several organs, such as the prostate and colon [54]. This addresses the possibility of whether any screening strategy should focus solely on early lesions or should additionally consider the tumor’s behavior. In the last two decades, detailed and comprehensive knowledge of cancer at the cellular and molecular levels has increased dramatically and exponentially, resulting in significant improvements in the characterization of human tumors, which has catalyzed a shift toward the development of targeted therapies, the foundation of molecular diagnostics [55, 56]. Omics technology may serve as the foundation for the development of novel cancer biomarker and/or panels that have significant advantages over currently utilized biomarkers. Omics has enhanced the number of potential biomarkers such as DNA, RNA, and other protein biomolecules that may be studied. The previous idea of single biomarker discovery has lately been supplanted by multi-biomarker discovery of a panel of genes or proteins, raising the question of whether heterogeneous and complex cancers can have a single fingerprint.
Biomarkers in association with cancer are used in oncology and clinical practice for risk assessment, screening, and diagnosis in combination with other diagnostic methods, and most importantly for determining prognosis and treatment response and/or recurrence. Cancer biomarkers can also help with cancer diagnosis at the molecular level. Clinicians and researchers must have a thorough understanding of the molecular aspects, clinical utility, and reliability of biomarkers in order to determine whether or not a biomarker is clinically useful for patient care and whether or not additional evaluation is required before integration into routine care. Biomarkers, through simplifying the integration of therapies and diagnostics, have the potential to play a key role in the development of customized medicine.
Research in the field of cancer-specific biomarkers have provided a promising source of novel diagnostic tools. Various groups have reported that altered cancer-associated biomarkers can be exploited to diagnose and monitor various cancers with greater sensitivity and specificity. Assessment of genomic and transcriptomic biomarkers found to be potentially very sensitive approaches for discriminating between cancerous non-cancerous (benign) conditions. Besides, this one could detect cancers at a much earlier stage by quantitative analysis of potential biomarker associated with specific cancer. Given the possible diagnostic power of genomic, transcriptomic, proteomic, and metabolomic biomarkers, these are currently one of the most promising areas of research in the field of development of cancer prognostic and diagnostics devices.
IntechOpen aims to guarantee that original material is published while at the same time giving significant freedom to our Authors. We uphold a flexible Copyright Policy, guaranteeing that there is no transfer of copyright to the publisher and Authors retain exclusive copyright to their Work.
',metaTitle:"Publication Agreement - Monograph",metaDescription:"IntechOpen aims to guarantee that original material is published while at the same time giving significant freedom to our authors. For that matter, we uphold a flexible copyright policy meaning that there is no transfer of copyright to the publisher and authors retain exclusive copyright to their work.",metaKeywords:null,canonicalURL:"/page/publication-agreement-monograph",contentRaw:'[{"type":"htmlEditorComponent","content":"When submitting a manuscript, the Author is required to accept the Terms and Conditions set out in our Publication Agreement – Monographs/Compacts as follows:
\\n\\nCORRESPONDING AUTHOR'S GRANT OF RIGHTS
\\n\\nSubject to the following Article, the Author grants to IntechOpen, during the full term of copyright, and any extensions or renewals of that term, the following:
\\n\\nThe foregoing licenses shall survive the expiry or termination of this Publication Agreement for any reason.
\\n\\nThe Author, on his or her own behalf and on behalf of any of the Co-Authors, reserves the following rights in the Work but agrees not to exercise them in such a way as to adversely affect IntechOpen's ability to utilize the full benefit of this Publication Agreement: (i) reprographic rights worldwide, other than those which subsist in the typographical arrangement of the Work as published by IntechOpen; and (ii) public lending rights arising under the Public Lending Right Act 1979, as amended from time to time, and any similar rights arising in any part of the world.
\\n\\nThe Author, and any Co-Author, confirms that they are, and will remain, a member of any applicable licensing and collecting society and any successor to that body responsible for administering royalties for the reprographic reproduction of copyright works.
\\n\\nSubject to the license granted above, copyright in the Work and all versions of it created during IntechOpen's editing process, including all published versions, is retained by the Author and any Co-Authors.
\\n\\nSubject to the license granted above, the Author and Co-Authors retain patent, trademark and other intellectual property rights to the Work.
\\n\\nAll rights granted to IntechOpen in this Article are assignable, sublicensable or otherwise transferrable to third parties without the specific approval of the Author or Co-Authors.
\\n\\nThe Author, on his/her own behalf and on behalf of the Co-Authors, will not assert any rights under the Copyright, Designs and Patents Act 1988 to object to derogatory treatment of the Work as a consequence of IntechOpen's changes to the Work arising from the translation of it, corrections and edits for house style, removal of problematic material and other reasonable edits as determined by IntechOpen.
\\n\\nAUTHOR'S DUTIES
\\n\\nWhen distributing or re-publishing the Work, the Author agrees to credit the Monograph/Compacts as the source of first publication, as well as IntechOpen. The Author guarantees that Co-Authors will also credit the Monograph/Compacts as the source of first publication, as well as IntechOpen, when they are distributing or re-publishing the Work.
\\n\\nThe Author agrees to:
\\n\\nThe Author will be held responsible for the payment of the agreed Open Access Publishing Fee before the completion of the project (Monograph/Compacts publication).
\\n\\nAll payments shall be due 30 days from the date of issue of the invoice. The Author or whoever is paying on behalf of the Author and Co-Authors will bear all banking and similar charges incurred.
\\n\\nThe Author shall obtain in writing all consents necessary for the reproduction of any material in which a third-party right exists, including quotations, photographs and illustrations, in all editions of the Work worldwide for the full term of the above licenses, and shall provide to IntechOpen, at its request, the original copies of such consents for inspection or the photocopies of such consents.
\\n\\nThe Author shall obtain written informed consent for publication from those who might recognize themselves or be identified by others, for example from case reports or photographs.
\\n\\nThe Author shall respect confidentiality during and after the termination of this Agreement. The information contained in all correspondence and documents as part of the publishing activity between IntechOpen and the Author and Co-Authors are confidential and are intended only for the recipients. The contents of any communication may not be disclosed publicly and are not intended for unauthorized use or distribution. Any use, disclosure, copying, or distribution is prohibited and may be unlawful.
\\n\\nAUTHOR'S WARRANTY
\\n\\nThe Author and Co-Authors confirm and warrant that the Work does not and will not breach any applicable law or the rights of any third party and, specifically, that the Work contains no matter that is defamatory or that infringes any literary or proprietary rights, intellectual property rights, or any rights of privacy.
\\n\\nThe Author and Co-Authors confirm that: (i) the Work is their original work and is not copied wholly or substantially from any other work or material or any other source; (ii) the Work has not been formally published in any other peer-reviewed journal or in a book or edited collection, and is not under consideration for any such publication; (iii) Authors and any applicable Co-Authors are qualifying persons under section 154 of the Copyright, Designs and Patents Act 1988; (iv) Authors and any applicable Co-Authors have not assigned, and will not during the term of this Publication Agreement purport to assign, any of the rights granted to IntechOpen under this Publication Agreement; and (v) the rights granted by this Publication Agreement are free from any security interest, option, mortgage, charge or lien.
\\n\\nThe Author and Co-Authors also confirm and warrant that: (i) he/she has the power to enter into this Publication Agreement on his or her own behalf and on behalf of each Co-Author; and (ii) has the necessary rights and/or title in and to the Work to grant IntechOpen, on behalf of themselves and any Co-Author, the rights and licences in this Publication Agreement. If the Work was prepared jointly by the Author and Co-Authors, the Author confirms that: (i) all Co-Authors agree to the submission, license and publication of the Work on the terms of this Publication Agreement; and (ii) the Author has the authority to enter into this biding Publication Agreement on behalf of each Co-Author. The Author shall: (i) ensure each Co-Author complies with all relevant provisions of this Publication Agreement, including those relating to confidentiality, performance and standards, as if a party to this Publication Agreement; and (ii) remain primarily liable for all acts and/or omissions of each Co-Author.
\\n\\nThe Author agrees to indemnify IntechOpen harmless against all liabilities, costs, expenses, damages and losses, as well as all reasonable legal costs and expenses suffered or incurred by IntechOpen arising out of, or in connection with, any breach of the agreed confirmations and warranties. This indemnity shall not apply in a situation in which a claim results from IntechOpen's negligence or willful misconduct.
\\n\\nNothing in this Publication Agreement shall have the effect of excluding or limiting any liability for death or personal injury caused by negligence or any other liability that cannot be excluded or limited by applicable law.
\\n\\nTERMINATION
\\n\\nIntechOpen has the right to terminate this Publication Agreement for quality, program, technical or other reasons with immediate effect, including without limitation (i) if the Author and/or any Co-Author commits a material breach of this Publication Agreement; (ii) if the Author and/or any Co-Author (being a private individual) is the subject of a bankruptcy petition, application or order; or (iii) if the Author and/or any Co-Author (as a corporate entity) commences negotiations with all or any class of its creditors with a view to rescheduling any of its debts, or makes a proposal for, or enters into, any compromise or arrangement with any of its creditors.
\\n\\nIn the event of termination, IntechOpen will notify the Author of the decision in writing.
\\n\\nIntechOpen’s DUTIES AND RIGHTS
\\n\\nUnless prevented from doing so by events beyond its reasonable control, IntechOpen, at its discretion, agrees to publish the Work attributing it to the Author and Co-Authors.
\\n\\nUnless prevented from doing so by events beyond its reasonable control, IntechOpen agrees to provide publishing services which include: managing editing (editorial and publishing process coordination, Author assistance); publishing software technology; language copyediting; typesetting; online publishing; hosting and web management; and abstracting and indexing services.
\\n\\nIntechOpen agrees to offer free online access to readers and use reasonable efforts to promote the Publication to relevant audiences.
\\n\\nIntechOpen is granted the authority to enforce the rights from this Publication Agreement on behalf of the Author and Co-Authors against third parties, for example in cases of plagiarism or copyright infringements. In respect of any such infringement or suspected infringement of the copyright in the Work, IntechOpen shall have absolute discretion in addressing any such infringement that is likely to affect IntechOpen's rights under this Publication Agreement, including issuing and conducting proceedings against the suspected infringer.
\\n\\nIntechOpen has the right to include/use the Author and Co-Authors names and likeness in connection with scientific dissemination, retrieval, archiving, web hosting and promotion and marketing of the Work and has the right to contact the Author and Co-Authors until the Work is publicly available on any platform owned and/or operated by IntechOpen.
\\n\\nMISCELLANEOUS
\\n\\nFurther Assurance: The Author shall ensure that any relevant third party, including any Co-Author, shall execute and deliver whatever further documents or deeds and perform such acts as IntechOpen reasonably requires from time to time for the purpose of giving IntechOpen the full benefit of the provisions of this Publication Agreement.
\\n\\nThird Party Rights: A person who is not a party to this Publication Agreement may not enforce any of its provisions under the Contracts (Rights of Third Parties) Act 1999.
\\n\\nEntire Agreement: This Publication Agreement constitutes the entire agreement between the parties in relation to its subject matter. It replaces all prior agreements, draft agreements, arrangements, collateral warranties, collateral contracts, statements, assurances, representations and undertakings of any nature made by, or on behalf of, the parties, whether oral or written, in relation to that subject matter. Each party acknowledges that in entering into this Publication Agreement it has not relied upon any oral or written statements, collateral or other warranties, assurances, representations or undertakings which were made by or on behalf of the other party in relation to the subject matter of this Publication Agreement at any time before its signature (known as the "Pre-Contractual Statements"), other than those which are set out in this Publication Agreement. Each party hereby waives all rights and remedies which might otherwise be available to it in relation to such Pre-Contractual Statements. Nothing in this clause shall exclude or restrict the liability of either party arising out of any fraudulent pre-contract misrepresentation or concealment.
\\n\\nWaiver: No failure or delay by a party to exercise any right or remedy provided under this Publication Agreement or by law shall constitute a waiver of that or any other right or remedy, nor shall it preclude or restrict the further exercise of that or any other right or remedy. No single or partial exercise of such right or remedy shall preclude or restrict the further exercise of that or any other right or remedy.
\\n\\nVariation: No variation of this Publication Agreement shall have effect unless it is in writing and signed by the parties, or their duly authorized representatives.
\\n\\nSeverance: If any provision, or part-provision, of this Publication Agreement is, or becomes invalid, illegal or unenforceable, it shall be deemed modified to the minimum extent necessary to make it valid, legal and enforceable. If such modification is not possible, the relevant provision or part-provision shall be deemed deleted. Any modification to, or deletion of, a provision or part-provision under this clause shall not affect the validity and enforceability of the rest of this Publication Agreement.
\\n\\nNo partnership: Nothing in this Publication Agreement is intended to, or shall be deemed to, establish or create any partnership or joint venture or the relationship of principal and agent or employer and employee between IntechOpen and the Author or any Co-Author, nor authorize any party to make or enter into any commitments for, or on behalf of, any other party.
\\n\\nGoverning law: This Publication Agreement and any dispute or claim, including non-contractual disputes or claims arising out of, or in connection with it, or its subject matter or formation, shall be governed by and construed in accordance with the law of England and Wales. The parties submit to the exclusive jurisdiction of the English courts to settle any dispute or claim arising out of, or in connection with, this Publication Agreement, including any non-contractual disputes or claims.
\\n\\nPolicy last updated: 2018-09-11
\\n"}]'},components:[{type:"htmlEditorComponent",content:'When submitting a manuscript, the Author is required to accept the Terms and Conditions set out in our Publication Agreement – Monographs/Compacts as follows:
\n\nCORRESPONDING AUTHOR'S GRANT OF RIGHTS
\n\nSubject to the following Article, the Author grants to IntechOpen, during the full term of copyright, and any extensions or renewals of that term, the following:
\n\nThe foregoing licenses shall survive the expiry or termination of this Publication Agreement for any reason.
\n\nThe Author, on his or her own behalf and on behalf of any of the Co-Authors, reserves the following rights in the Work but agrees not to exercise them in such a way as to adversely affect IntechOpen's ability to utilize the full benefit of this Publication Agreement: (i) reprographic rights worldwide, other than those which subsist in the typographical arrangement of the Work as published by IntechOpen; and (ii) public lending rights arising under the Public Lending Right Act 1979, as amended from time to time, and any similar rights arising in any part of the world.
\n\nThe Author, and any Co-Author, confirms that they are, and will remain, a member of any applicable licensing and collecting society and any successor to that body responsible for administering royalties for the reprographic reproduction of copyright works.
\n\nSubject to the license granted above, copyright in the Work and all versions of it created during IntechOpen's editing process, including all published versions, is retained by the Author and any Co-Authors.
\n\nSubject to the license granted above, the Author and Co-Authors retain patent, trademark and other intellectual property rights to the Work.
\n\nAll rights granted to IntechOpen in this Article are assignable, sublicensable or otherwise transferrable to third parties without the specific approval of the Author or Co-Authors.
\n\nThe Author, on his/her own behalf and on behalf of the Co-Authors, will not assert any rights under the Copyright, Designs and Patents Act 1988 to object to derogatory treatment of the Work as a consequence of IntechOpen's changes to the Work arising from the translation of it, corrections and edits for house style, removal of problematic material and other reasonable edits as determined by IntechOpen.
\n\nAUTHOR'S DUTIES
\n\nWhen distributing or re-publishing the Work, the Author agrees to credit the Monograph/Compacts as the source of first publication, as well as IntechOpen. The Author guarantees that Co-Authors will also credit the Monograph/Compacts as the source of first publication, as well as IntechOpen, when they are distributing or re-publishing the Work.
\n\nThe Author agrees to:
\n\nThe Author will be held responsible for the payment of the agreed Open Access Publishing Fee before the completion of the project (Monograph/Compacts publication).
\n\nAll payments shall be due 30 days from the date of issue of the invoice. The Author or whoever is paying on behalf of the Author and Co-Authors will bear all banking and similar charges incurred.
\n\nThe Author shall obtain in writing all consents necessary for the reproduction of any material in which a third-party right exists, including quotations, photographs and illustrations, in all editions of the Work worldwide for the full term of the above licenses, and shall provide to IntechOpen, at its request, the original copies of such consents for inspection or the photocopies of such consents.
\n\nThe Author shall obtain written informed consent for publication from those who might recognize themselves or be identified by others, for example from case reports or photographs.
\n\nThe Author shall respect confidentiality during and after the termination of this Agreement. The information contained in all correspondence and documents as part of the publishing activity between IntechOpen and the Author and Co-Authors are confidential and are intended only for the recipients. The contents of any communication may not be disclosed publicly and are not intended for unauthorized use or distribution. Any use, disclosure, copying, or distribution is prohibited and may be unlawful.
\n\nAUTHOR'S WARRANTY
\n\nThe Author and Co-Authors confirm and warrant that the Work does not and will not breach any applicable law or the rights of any third party and, specifically, that the Work contains no matter that is defamatory or that infringes any literary or proprietary rights, intellectual property rights, or any rights of privacy.
\n\nThe Author and Co-Authors confirm that: (i) the Work is their original work and is not copied wholly or substantially from any other work or material or any other source; (ii) the Work has not been formally published in any other peer-reviewed journal or in a book or edited collection, and is not under consideration for any such publication; (iii) Authors and any applicable Co-Authors are qualifying persons under section 154 of the Copyright, Designs and Patents Act 1988; (iv) Authors and any applicable Co-Authors have not assigned, and will not during the term of this Publication Agreement purport to assign, any of the rights granted to IntechOpen under this Publication Agreement; and (v) the rights granted by this Publication Agreement are free from any security interest, option, mortgage, charge or lien.
\n\nThe Author and Co-Authors also confirm and warrant that: (i) he/she has the power to enter into this Publication Agreement on his or her own behalf and on behalf of each Co-Author; and (ii) has the necessary rights and/or title in and to the Work to grant IntechOpen, on behalf of themselves and any Co-Author, the rights and licences in this Publication Agreement. If the Work was prepared jointly by the Author and Co-Authors, the Author confirms that: (i) all Co-Authors agree to the submission, license and publication of the Work on the terms of this Publication Agreement; and (ii) the Author has the authority to enter into this biding Publication Agreement on behalf of each Co-Author. The Author shall: (i) ensure each Co-Author complies with all relevant provisions of this Publication Agreement, including those relating to confidentiality, performance and standards, as if a party to this Publication Agreement; and (ii) remain primarily liable for all acts and/or omissions of each Co-Author.
\n\nThe Author agrees to indemnify IntechOpen harmless against all liabilities, costs, expenses, damages and losses, as well as all reasonable legal costs and expenses suffered or incurred by IntechOpen arising out of, or in connection with, any breach of the agreed confirmations and warranties. This indemnity shall not apply in a situation in which a claim results from IntechOpen's negligence or willful misconduct.
\n\nNothing in this Publication Agreement shall have the effect of excluding or limiting any liability for death or personal injury caused by negligence or any other liability that cannot be excluded or limited by applicable law.
\n\nTERMINATION
\n\nIntechOpen has the right to terminate this Publication Agreement for quality, program, technical or other reasons with immediate effect, including without limitation (i) if the Author and/or any Co-Author commits a material breach of this Publication Agreement; (ii) if the Author and/or any Co-Author (being a private individual) is the subject of a bankruptcy petition, application or order; or (iii) if the Author and/or any Co-Author (as a corporate entity) commences negotiations with all or any class of its creditors with a view to rescheduling any of its debts, or makes a proposal for, or enters into, any compromise or arrangement with any of its creditors.
\n\nIn the event of termination, IntechOpen will notify the Author of the decision in writing.
\n\nIntechOpen’s DUTIES AND RIGHTS
\n\nUnless prevented from doing so by events beyond its reasonable control, IntechOpen, at its discretion, agrees to publish the Work attributing it to the Author and Co-Authors.
\n\nUnless prevented from doing so by events beyond its reasonable control, IntechOpen agrees to provide publishing services which include: managing editing (editorial and publishing process coordination, Author assistance); publishing software technology; language copyediting; typesetting; online publishing; hosting and web management; and abstracting and indexing services.
\n\nIntechOpen agrees to offer free online access to readers and use reasonable efforts to promote the Publication to relevant audiences.
\n\nIntechOpen is granted the authority to enforce the rights from this Publication Agreement on behalf of the Author and Co-Authors against third parties, for example in cases of plagiarism or copyright infringements. In respect of any such infringement or suspected infringement of the copyright in the Work, IntechOpen shall have absolute discretion in addressing any such infringement that is likely to affect IntechOpen's rights under this Publication Agreement, including issuing and conducting proceedings against the suspected infringer.
\n\nIntechOpen has the right to include/use the Author and Co-Authors names and likeness in connection with scientific dissemination, retrieval, archiving, web hosting and promotion and marketing of the Work and has the right to contact the Author and Co-Authors until the Work is publicly available on any platform owned and/or operated by IntechOpen.
\n\nMISCELLANEOUS
\n\nFurther Assurance: The Author shall ensure that any relevant third party, including any Co-Author, shall execute and deliver whatever further documents or deeds and perform such acts as IntechOpen reasonably requires from time to time for the purpose of giving IntechOpen the full benefit of the provisions of this Publication Agreement.
\n\nThird Party Rights: A person who is not a party to this Publication Agreement may not enforce any of its provisions under the Contracts (Rights of Third Parties) Act 1999.
\n\nEntire Agreement: This Publication Agreement constitutes the entire agreement between the parties in relation to its subject matter. It replaces all prior agreements, draft agreements, arrangements, collateral warranties, collateral contracts, statements, assurances, representations and undertakings of any nature made by, or on behalf of, the parties, whether oral or written, in relation to that subject matter. Each party acknowledges that in entering into this Publication Agreement it has not relied upon any oral or written statements, collateral or other warranties, assurances, representations or undertakings which were made by or on behalf of the other party in relation to the subject matter of this Publication Agreement at any time before its signature (known as the "Pre-Contractual Statements"), other than those which are set out in this Publication Agreement. Each party hereby waives all rights and remedies which might otherwise be available to it in relation to such Pre-Contractual Statements. Nothing in this clause shall exclude or restrict the liability of either party arising out of any fraudulent pre-contract misrepresentation or concealment.
\n\nWaiver: No failure or delay by a party to exercise any right or remedy provided under this Publication Agreement or by law shall constitute a waiver of that or any other right or remedy, nor shall it preclude or restrict the further exercise of that or any other right or remedy. No single or partial exercise of such right or remedy shall preclude or restrict the further exercise of that or any other right or remedy.
\n\nVariation: No variation of this Publication Agreement shall have effect unless it is in writing and signed by the parties, or their duly authorized representatives.
\n\nSeverance: If any provision, or part-provision, of this Publication Agreement is, or becomes invalid, illegal or unenforceable, it shall be deemed modified to the minimum extent necessary to make it valid, legal and enforceable. If such modification is not possible, the relevant provision or part-provision shall be deemed deleted. Any modification to, or deletion of, a provision or part-provision under this clause shall not affect the validity and enforceability of the rest of this Publication Agreement.
\n\nNo partnership: Nothing in this Publication Agreement is intended to, or shall be deemed to, establish or create any partnership or joint venture or the relationship of principal and agent or employer and employee between IntechOpen and the Author or any Co-Author, nor authorize any party to make or enter into any commitments for, or on behalf of, any other party.
\n\nGoverning law: This Publication Agreement and any dispute or claim, including non-contractual disputes or claims arising out of, or in connection with it, or its subject matter or formation, shall be governed by and construed in accordance with the law of England and Wales. The parties submit to the exclusive jurisdiction of the English courts to settle any dispute or claim arising out of, or in connection with, this Publication Agreement, including any non-contractual disputes or claims.
\n\nPolicy last updated: 2018-09-11
\n'}]},successStories:{items:[]},authorsAndEditors:{filterParams:{sort:"featured,name"},profiles:[],filtersByRegion:[],offset:0,limit:12,total:null},chapterEmbeded:{data:{}},editorApplication:{success:null,errors:{}},ofsBooks:{filterParams:{hasNoEditors:"0",sort:"dateEndThirdStepPublish",topicId:"8,9,10,11,14,15,20,22,24"},books:[{type:"book",id:"11124",title:"Next-Generation Textiles",subtitle:null,isOpenForSubmission:!0,hash:"093f9e26bb829b8d414d13626aea1086",slug:null,bookSignature:"Dr. Hassan Ibrahim",coverURL:"https://cdn.intechopen.com/books/images_new/11124.jpg",editedByType:null,editors:[{id:"90645",title:"Dr.",name:"Hassan",surname:"Ibrahim",slug:"hassan-ibrahim",fullName:"Hassan Ibrahim"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"11561",title:"Zeolite From Wastes - New Perspectives on Innovative Resources and Their Valorization Process",subtitle:null,isOpenForSubmission:!0,hash:"3ed0dfd842de9cd1143212415903e6ad",slug:null,bookSignature:"Dr. Claudia Belviso",coverURL:"https://cdn.intechopen.com/books/images_new/11561.jpg",editedByType:null,editors:[{id:"61457",title:"Dr.",name:"Claudia",surname:"Belviso",slug:"claudia-belviso",fullName:"Claudia Belviso"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"11922",title:"Watermarking - Recent Advances, New Perspectives and Applications",subtitle:null,isOpenForSubmission:!0,hash:"9843dc1d810407088ed9eef10768a64b",slug:null,bookSignature:"Prof. Joceli Mayer",coverURL:"https://cdn.intechopen.com/books/images_new/11922.jpg",editedByType:null,editors:[{id:"110638",title:"Prof.",name:"Joceli",surname:"Mayer",slug:"joceli-mayer",fullName:"Joceli Mayer"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"11762",title:"Characteristics and Applications of Boron",subtitle:null,isOpenForSubmission:!0,hash:"611776f7f3cc9951a8956d2e3d535a8e",slug:null,bookSignature:"Associate Prof. Chatchawal Wongchoosuk",coverURL:"https://cdn.intechopen.com/books/images_new/11762.jpg",editedByType:null,editors:[{id:"34521",title:"Associate Prof.",name:"Chatchawal",surname:"Wongchoosuk",slug:"chatchawal-wongchoosuk",fullName:"Chatchawal Wongchoosuk"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"11910",title:"Frontiers in Voltammetry",subtitle:null,isOpenForSubmission:!0,hash:"fc53a7599a61ed04a0672a7bca81e9c2",slug:null,bookSignature:"Dr. Rajendrachari Shashanka, Dr. Kiran Kenchappa Somashekharappa, Dr. Sharath Peramenahalli Chikkegouda and Dr. Shamanth Vasanth",coverURL:"https://cdn.intechopen.com/books/images_new/11910.jpg",editedByType:null,editors:[{id:"246025",title:"Dr.",name:"Shashanka",surname:"Rajendrachari",slug:"shashanka-rajendrachari",fullName:"Shashanka Rajendrachari"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"11517",title:"Phase Change Materials - Technology and Applications",subtitle:null,isOpenForSubmission:!0,hash:"1b7a5f2631db5e49399539ade1edf264",slug:null,bookSignature:"Dr. Manish K Rathod",coverURL:"https://cdn.intechopen.com/books/images_new/11517.jpg",editedByType:null,editors:[{id:"236035",title:"Dr.",name:"Manish",surname:"Rathod",slug:"manish-rathod",fullName:"Manish Rathod"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"11451",title:"Molecular Docking - Recent Advances",subtitle:null,isOpenForSubmission:!0,hash:"8c918a1973786c7059752b28601f1329",slug:null,bookSignature:"Dr. Erman Salih Istifli",coverURL:"https://cdn.intechopen.com/books/images_new/11451.jpg",editedByType:null,editors:[{id:"179007",title:"Dr.",name:"Erman Salih",surname:"Istifli",slug:"erman-salih-istifli",fullName:"Erman Salih Istifli"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"11932",title:"New Materials and Enhanced Performance of Sodium-Ion Batteries",subtitle:null,isOpenForSubmission:!0,hash:"75c27a6f2739e8af817bace95b0e50d6",slug:null,bookSignature:"Ph.D. Fatma SARF",coverURL:"https://cdn.intechopen.com/books/images_new/11932.jpg",editedByType:null,editors:[{id:"245850",title:"Ph.D.",name:"Fatma",surname:"SARF",slug:"fatma-sarf",fullName:"Fatma SARF"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"11528",title:"Maintenance Management - Current Challenges, New Developments, and Future Directions",subtitle:null,isOpenForSubmission:!0,hash:"a3e4ad5806a77b0e930fbd4cb191bee2",slug:null,bookSignature:"Prof. Germano Lambert-Torres, Dr. Erik Leandro Bonaldi and Dr. Levy Ely Oliveira",coverURL:"https://cdn.intechopen.com/books/images_new/11528.jpg",editedByType:null,editors:[{id:"112971",title:"Prof.",name:"Germano",surname:"Lambert-Torres",slug:"germano-lambert-torres",fullName:"Germano Lambert-Torres"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"11509",title:"Wireless Power Transfer - Perspectives and Application",subtitle:null,isOpenForSubmission:!0,hash:"f188555eee4211fc24b6cca361983149",slug:null,bookSignature:"Dr. Kim Ho Yeap",coverURL:"https://cdn.intechopen.com/books/images_new/11509.jpg",editedByType:null,editors:[{id:"126825",title:"Dr.",name:"Kim Ho",surname:"Yeap",slug:"kim-ho-yeap",fullName:"Kim Ho Yeap"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"11760",title:"Applications and Use of Diamond",subtitle:null,isOpenForSubmission:!0,hash:"2edcf9a24450d8655e756e1080defe32",slug:null,bookSignature:"Mr. Evgeniy Lipatov",coverURL:"https://cdn.intechopen.com/books/images_new/11760.jpg",editedByType:null,editors:[{id:"21254",title:"Mr.",name:"Evgeniy",surname:"Lipatov",slug:"evgeniy-lipatov",fullName:"Evgeniy Lipatov"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"11486",title:"Climate Change - Recent Observations",subtitle:null,isOpenForSubmission:!0,hash:"741543ff220f5cf688efbf12d3e2f536",slug:null,bookSignature:"Assistant Prof. Terence Epule Epule",coverURL:"https://cdn.intechopen.com/books/images_new/11486.jpg",editedByType:null,editors:[{id:"348146",title:"Assistant Prof.",name:"Terence Epule",surname:"Epule",slug:"terence-epule-epule",fullName:"Terence Epule Epule"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}}],filtersByTopic:[{group:"topic",caption:"Agricultural and Biological Sciences",value:5,count:38},{group:"topic",caption:"Biochemistry, Genetics and Molecular Biology",value:6,count:13},{group:"topic",caption:"Business, Management and Economics",value:7,count:7},{group:"topic",caption:"Chemistry",value:8,count:23},{group:"topic",caption:"Computer and Information Science",value:9,count:24},{group:"topic",caption:"Earth and Planetary Sciences",value:10,count:15},{group:"topic",caption:"Engineering",value:11,count:65},{group:"topic",caption:"Environmental Sciences",value:12,count:10},{group:"topic",caption:"Immunology and Microbiology",value:13,count:16},{group:"topic",caption:"Materials Science",value:14,count:25},{group:"topic",caption:"Mathematics",value:15,count:11},{group:"topic",caption:"Medicine",value:16,count:116},{group:"topic",caption:"Nanotechnology and Nanomaterials",value:17,count:6},{group:"topic",caption:"Neuroscience",value:18,count:4},{group:"topic",caption:"Pharmacology, Toxicology and Pharmaceutical Science",value:19,count:9},{group:"topic",caption:"Physics",value:20,count:9},{group:"topic",caption:"Psychology",value:21,count:10},{group:"topic",caption:"Robotics",value:22,count:2},{group:"topic",caption:"Social Sciences",value:23,count:9},{group:"topic",caption:"Veterinary Medicine and Science",value:25,count:4}],offset:12,limit:12,total:174},popularBooks:{featuredBooks:[],offset:0,limit:12,total:null},hotBookTopics:{hotBooks:[],offset:0,limit:12,total:null},publish:{},publishingProposal:{success:null,errors:{}},books:{featuredBooks:[],latestBooks:[]},subject:{topic:{id:"872",title:"Water Resources",slug:"water-resources",parent:{id:"136",title:"Environmental Sustainability",slug:"environmental-sciences-environmental-sustainability"},numberOfBooks:7,numberOfSeries:0,numberOfAuthorsAndEditors:128,numberOfWosCitations:8,numberOfCrossrefCitations:23,numberOfDimensionsCitations:43,videoUrl:null,fallbackUrl:null,description:null},booksByTopicFilter:{topicId:"872",sort:"-publishedDate",limit:12,offset:0},booksByTopicCollection:[{type:"book",id:"10682",title:"Pathways and Challenges for Efficient Desalination",subtitle:null,isOpenForSubmission:!1,hash:"ca25e9eca70d54deb503d2663f75218c",slug:"pathways-and-challenges-for-efficient-desalination",bookSignature:"Muhammad Wakil Shahzad, Mike Dixon, Giancarlo Barassi, Ben Bin Xu and Yinzhu Jiang",coverURL:"https://cdn.intechopen.com/books/images_new/10682.jpg",editedByType:"Edited by",editors:[{id:"174208",title:"Dr.",name:"Muhammad Wakil",middleName:null,surname:"Shahzad",slug:"muhammad-wakil-shahzad",fullName:"Muhammad Wakil Shahzad"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"10976",title:"Water Conservation",subtitle:"Inevitable Strategy",isOpenForSubmission:!1,hash:"2a5f75a1eed9cb67133fe6ce0f8848f5",slug:"water-conservation-inevitable-strategy",bookSignature:"Murat Eyvaz, Ahmed Albahnasawi, Ercan Gürbulak and Ebubekir Yüksel",coverURL:"https://cdn.intechopen.com/books/images_new/10976.jpg",editedByType:"Edited by",editors:[{id:"170083",title:"Associate Prof.",name:"Murat",middleName:null,surname:"Eyvaz",slug:"murat-eyvaz",fullName:"Murat Eyvaz"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"11074",title:"Water Quality",subtitle:"Factors and Impacts",isOpenForSubmission:!1,hash:"c3f3c2405260fed102e4ef982cff54c6",slug:"water-quality-factors-and-impacts",bookSignature:"Daniel Dunea",coverURL:"https://cdn.intechopen.com/books/images_new/11074.jpg",editedByType:"Edited by",editors:[{id:"180202",title:"Associate Prof.",name:"Daniel",middleName:null,surname:"Dunea",slug:"daniel-dunea",fullName:"Daniel Dunea"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"9981",title:"Groundwater Management and Resources",subtitle:null,isOpenForSubmission:!1,hash:"4f408ce58f029e746911fe558ca4bbd0",slug:"groundwater-management-and-resources",bookSignature:"Bahareh Kalantar",coverURL:"https://cdn.intechopen.com/books/images_new/9981.jpg",editedByType:"Edited by",editors:[{id:"267005",title:"Dr.",name:"Bahareh",middleName:null,surname:"Kalantar",slug:"bahareh-kalantar",fullName:"Bahareh Kalantar"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"8098",title:"Resources of Water",subtitle:null,isOpenForSubmission:!1,hash:"d251652996624d932ef7b8ed62cf7cfc",slug:"resources-of-water",bookSignature:"Prathna Thanjavur Chandrasekaran, Muhammad Salik Javaid, Aftab Sadiq",coverURL:"https://cdn.intechopen.com/books/images_new/8098.jpg",editedByType:"Edited by",editors:[{id:"167917",title:"Dr.",name:"Prathna",middleName:null,surname:"Thanjavur Chandrasekaran",slug:"prathna-thanjavur-chandrasekaran",fullName:"Prathna Thanjavur Chandrasekaran"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"6886",title:"Water and Sustainability",subtitle:null,isOpenForSubmission:!1,hash:"f01b34abf4b3d3329dda4921c461fcf4",slug:"water-and-sustainability",bookSignature:"Prathna Thanjavur Chandrasekaran",coverURL:"https://cdn.intechopen.com/books/images_new/6886.jpg",editedByType:"Edited by",editors:[{id:"167917",title:"Dr.",name:"Prathna",middleName:null,surname:"Thanjavur Chandrasekaran",slug:"prathna-thanjavur-chandrasekaran",fullName:"Prathna Thanjavur Chandrasekaran"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"6184",title:"Applications in Water Systems Management and Modeling",subtitle:null,isOpenForSubmission:!1,hash:"fba712d6246fe9d253e35b1cdb8cd972",slug:"applications-in-water-systems-management-and-modeling",bookSignature:"Daniela Malcangio",coverURL:"https://cdn.intechopen.com/books/images_new/6184.jpg",editedByType:"Edited by",editors:[{id:"205577",title:"Dr.",name:"Daniela",middleName:null,surname:"Malcangio",slug:"daniela-malcangio",fullName:"Daniela Malcangio"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}}],booksByTopicTotal:7,seriesByTopicCollection:[],seriesByTopicTotal:0,mostCitedChapters:[{id:"58856",doi:"10.5772/intechopen.72226",title:"The Effects of Climate Change on Rural-Urban Migration in Sub-Saharan Africa (SSA)—The Cases of Democratic Republic of Congo, Kenya and Niger",slug:"the-effects-of-climate-change-on-rural-urban-migration-in-sub-saharan-africa-ssa-the-cases-of-democr",totalDownloads:1867,totalCrossrefCites:4,totalDimensionsCites:6,abstract:"Water is essential for the existence of living organisms including humans. Water is needed in farms to grow crops, firms and manufacturing industry to produce products and services. This chapter examines water resources availability and management in Sub-Saharan Africa (SSA) in climate change perspective using vector auto-regression (VAR) time series analysis. Water is known to be unevenly distributed among countries and continents around the world, particularly in Sub-Sahara Africa; the water availability varies between member countries and regions in the individual country, water supply systems experience enormous pressure to make water accessible to people in both rural and urban communities. Water security remains to be an integral part of the SSA’s effort to achieve food security and supply, halve poverty and eradicate hunger. This chapter more importantly aims to investigate impact of rainfall and temperature issues––that are climate change proxy variables––on water security and people movement in three Sub-Saharan African countries that are Democratic Republic of Congo, Kenya and Niger. This article assesses some possible causes of migration from rural to urban area using VAR and granger causality tests; this process involves four variables namely Rural Migration ‘MR’, Urban Migration ‘MU’, Rainfall ‘Rain’ and Temperature ‘Temp’. The model predicts rainfall and temperature across 10 years and examines how these changes impact water availability and people movement in relevant countries. This study finds that some countries are experiencing water security challenges upon which large numbers migrate to urban areas. The study reveals that variations in rainfall and temperature have compounded people movements from rural areas. It is noted that the agricultural production in SSA have not improved over time and in fact, it has further decreased due to the move away from rural areas by many farmers.",book:{id:"6184",slug:"applications-in-water-systems-management-and-modeling",title:"Applications in Water Systems Management and Modeling",fullTitle:"Applications in Water Systems Management and Modeling"},signatures:"Omar Moalin Hassan and Gurudeo Anand Tularam",authors:[{id:"148090",title:"Dr.",name:"Gurudeo",middleName:null,surname:"Tularam",slug:"gurudeo-tularam",fullName:"Gurudeo Tularam"},{id:"208956",title:"Mr.",name:"Omar",middleName:null,surname:"Moalin Hassan",slug:"omar-moalin-hassan",fullName:"Omar Moalin Hassan"}]},{id:"60177",doi:"10.5772/intechopen.74914",title:"Application of a Hydrodynamic and Water Quality Model for Inland Surface Water Systems",slug:"application-of-a-hydrodynamic-and-water-quality-model-for-inland-surface-water-systems",totalDownloads:1731,totalCrossrefCites:3,totalDimensionsCites:6,abstract:"This chapter introduces basic concepts, properties, and principles of different processes in inland surface water and analytical methodologies. The fundamentals of surface water hydrodynamics, including water properties, hydrodynamic processes, Cartesian coordinate-based governing equations, and boundary and initial conditions were reviewed. The fate and transport of contaminants in surface water were introduced. Based on aforementioned theory and principles, two hydrodynamic-water quality models were developed for studying a lake and a river, respectively. A stratified 3D model was used to investigate the circulation and E. coli transport in the nearshore region of Lake Michigan. The modeling results show that stratified phenomenon exists in the near region, and a 3D model is necessary even though a previous 2D model works well for the shallow water environment. A 2D depth-averaged water quality model was developed to estimate the fate and transport of four contaminants in the San Joaquin River of California. The modeling results indicate that it took 20 days for these contaminants to transport from the upstream to the downstream in the research domain. These models can be effectively used for inland surface water restoration and management.",book:{id:"6184",slug:"applications-in-water-systems-management-and-modeling",title:"Applications in Water Systems Management and Modeling",fullTitle:"Applications in Water Systems Management and Modeling"},signatures:"Lubo Liu",authors:[{id:"169118",title:"Dr.",name:"Lubo",middleName:null,surname:"Liu",slug:"lubo-liu",fullName:"Lubo Liu"}]},{id:"59309",doi:"10.5772/intechopen.73274",title:"Assessing the Hydrodynamic Pattern in Different Lakes of Malaysia",slug:"assessing-the-hydrodynamic-pattern-in-different-lakes-of-malaysia",totalDownloads:940,totalCrossrefCites:3,totalDimensionsCites:5,abstract:"Hydrodynamic simulations using three-dimensional numerical model were carried out in three different shallow tropical lakes to understand the characteristics of water movement in the respective water bodies. The models were based on meteorological data from the nearest stations and calibrated with current measurement, temperature, or water-level data. The results show good agreement between measured and simulated velocities and/or temperature at certain depth. This study found that the major driving forces of the hydrodynamic pattern were different in the three lakes. Hydrodynamic simulations showed that Bukit Merah and Durian Tunggal reservoirs were more sensitive to wind-driven motion. Floodplain lakes, such as Bera Lake, are more sensitive to flood inflow by the main river during the monsoon season. Convective motion driven by water temperature gradient was important for Bukit Merah and Bera Lake.",book:{id:"6184",slug:"applications-in-water-systems-management-and-modeling",title:"Applications in Water Systems Management and Modeling",fullTitle:"Applications in Water Systems Management and Modeling"},signatures:"Zati Sharip, Shahirwan Aman Shah, Aminuddin Jamin and Juhaimi\nJusoh",authors:[{id:"186369",title:"Dr.",name:"Zati",middleName:null,surname:"Sharip",slug:"zati-sharip",fullName:"Zati Sharip"},{id:"220302",title:"Mr.",name:"Shahirwan",middleName:null,surname:"Aman Shah",slug:"shahirwan-aman-shah",fullName:"Shahirwan Aman Shah"},{id:"220303",title:"Mr.",name:"Aminuddin",middleName:null,surname:"Jamin",slug:"aminuddin-jamin",fullName:"Aminuddin Jamin"},{id:"220304",title:"Mr.",name:"Juhaimi",middleName:null,surname:"Jusoh",slug:"juhaimi-jusoh",fullName:"Juhaimi Jusoh"}]},{id:"71359",doi:"10.5772/intechopen.90652",title:"Effects of Climate Change on Water Resources, Indices, and Related Activities in Colombia",slug:"effects-of-climate-change-on-water-resources-indices-and-related-activities-in-colombia",totalDownloads:713,totalCrossrefCites:3,totalDimensionsCites:4,abstract:"In Colombia, a country with great climatic diversity, the water balance is affected in one way or another by climate change depending on the region. Thus, there may be increases and decreases in precipitation and, in all cases, a huge increase in temperature. This document presents some studies carried out in different areas of the country regarding the effects of climate change on water resources, including its influence on hydroelectric power generation, some changes in the water balance in arid areas, and the opportunity to ensemble climate change scenarios. Likewise, it outlines a possible future water supply-demand relationship, where supply is associated with a change in the water balance and demand with some crops, activities, and sectors that need water to survive. This allows to estimate some future status indices to see the overall picture of climate change in connection with the country’s water resources.",book:{id:"8098",slug:"resources-of-water",title:"Resources of Water",fullTitle:"Resources of Water"},signatures:"Nathaly Güiza-Villa, Carlos Gay-García and Jesús Efren Ospina-Noreña",authors:[{id:"311362",title:"Ph.D.",name:"Jesús Efren",middleName:null,surname:"Ospina-Noreña",slug:"jesus-efren-ospina-norena",fullName:"Jesús Efren Ospina-Noreña"},{id:"311363",title:"Dr.",name:"Carlos",middleName:null,surname:"Gay-García",slug:"carlos-gay-garcia",fullName:"Carlos Gay-García"},{id:"311364",title:"M.Sc.",name:"Nathaly",middleName:null,surname:"Güiza-Villa",slug:"nathaly-guiza-villa",fullName:"Nathaly Güiza-Villa"}]},{id:"65487",doi:"10.5772/intechopen.82825",title:"Sustainable and Resilient Water and Energy Futures: From New Ethics and Choices to Urban Nexus Strategies",slug:"sustainable-and-resilient-water-and-energy-futures-from-new-ethics-and-choices-to-urban-nexus-strate",totalDownloads:1380,totalCrossrefCites:2,totalDimensionsCites:3,abstract:"A safe, secure and affordable water future—for life, health, economy—are foundational outcomes from a new form of ethics for water stewardship and energy management. Current business as usual in water and energy systems have not led to sustainable, healthy nor resilient pathways for urban and rural communities alike. Today, an estimated 400 million people live in cities with significant water shortages. This is while 25% of water is currently lost before even used in urban areas (up to 60% in some cities) due to aging infrastructure. In addition, on average, only 10% of wastewater is treated before returning to water bodies in developing countries. By 2040, more than 66% of the world’s populations could suffer from severe water shortages; and by 2050, an 80% increase in urban water demand (over current levels) may result in one billion city dwellers and 36% (one in three) of cities expected to face water crises. A crisis is often a catalyst for innovation and this chapter is a call to cities to enable strategic responses—moving away from legacy ‘siloed’ infrastructures, over-allocated water resources and emerging ethical dilemmas to integrated water- and energy-related urban nexus strategies.",book:{id:"6886",slug:"water-and-sustainability",title:"Water and Sustainability",fullTitle:"Water and Sustainability"},signatures:"Josh Sperling and Will Sarni",authors:null}],mostDownloadedChaptersLast30Days:[{id:"58856",title:"The Effects of Climate Change on Rural-Urban Migration in Sub-Saharan Africa (SSA)—The Cases of Democratic Republic of Congo, Kenya and Niger",slug:"the-effects-of-climate-change-on-rural-urban-migration-in-sub-saharan-africa-ssa-the-cases-of-democr",totalDownloads:1867,totalCrossrefCites:4,totalDimensionsCites:6,abstract:"Water is essential for the existence of living organisms including humans. Water is needed in farms to grow crops, firms and manufacturing industry to produce products and services. This chapter examines water resources availability and management in Sub-Saharan Africa (SSA) in climate change perspective using vector auto-regression (VAR) time series analysis. Water is known to be unevenly distributed among countries and continents around the world, particularly in Sub-Sahara Africa; the water availability varies between member countries and regions in the individual country, water supply systems experience enormous pressure to make water accessible to people in both rural and urban communities. Water security remains to be an integral part of the SSA’s effort to achieve food security and supply, halve poverty and eradicate hunger. This chapter more importantly aims to investigate impact of rainfall and temperature issues––that are climate change proxy variables––on water security and people movement in three Sub-Saharan African countries that are Democratic Republic of Congo, Kenya and Niger. This article assesses some possible causes of migration from rural to urban area using VAR and granger causality tests; this process involves four variables namely Rural Migration ‘MR’, Urban Migration ‘MU’, Rainfall ‘Rain’ and Temperature ‘Temp’. The model predicts rainfall and temperature across 10 years and examines how these changes impact water availability and people movement in relevant countries. This study finds that some countries are experiencing water security challenges upon which large numbers migrate to urban areas. The study reveals that variations in rainfall and temperature have compounded people movements from rural areas. It is noted that the agricultural production in SSA have not improved over time and in fact, it has further decreased due to the move away from rural areas by many farmers.",book:{id:"6184",slug:"applications-in-water-systems-management-and-modeling",title:"Applications in Water Systems Management and Modeling",fullTitle:"Applications in Water Systems Management and Modeling"},signatures:"Omar Moalin Hassan and Gurudeo Anand Tularam",authors:[{id:"148090",title:"Dr.",name:"Gurudeo",middleName:null,surname:"Tularam",slug:"gurudeo-tularam",fullName:"Gurudeo Tularam"},{id:"208956",title:"Mr.",name:"Omar",middleName:null,surname:"Moalin Hassan",slug:"omar-moalin-hassan",fullName:"Omar Moalin Hassan"}]},{id:"73528",title:"Characteristics and Assessment of Groundwater",slug:"characteristics-and-assessment-of-groundwater",totalDownloads:785,totalCrossrefCites:0,totalDimensionsCites:2,abstract:"Groundwater system is very vital to humanity and the ecosystem. Aquifers are determined based on the absence or presence of water table positioning, that is, confined, unconfined, leaky aquifers and fractured aquifers. The objective of this chapter is to discuss the characteristic and assessment of groundwater within the scope of vertical distribution of GW, types of the aquifer system, types of SW-GW interface, and SW-GW interaction at both local and regional scales. The properties of the aquifer depend on the physical characteristics of the materials (porosity, permeability, specific yield, specific storage, and hydraulic conductivities) which are determined by techniques like resistivity surveys and pumping tests followed by remote sensing and geographic information system for better information on the groundwater system. Furthermore, understanding the SW-GW interactions through available methods (seepage meter, heat tracer, and environmental tracer) is useful in watershed management, that is, risk management and assessment of the aquifer system.",book:{id:"9981",slug:"groundwater-management-and-resources",title:"Groundwater Management and Resources",fullTitle:"Groundwater Management and Resources"},signatures:"Naseem Akhtar, Muhammad Izzuddin Syakir, Mohd Talha Anees, Abdul Qadir and Mohamad Shaiful Yusuff",authors:[{id:"201647",title:"Mr.",name:"Mohd Talha",middleName:null,surname:"Anees",slug:"mohd-talha-anees",fullName:"Mohd Talha Anees"},{id:"203218",title:"Dr.",name:"Muhammad Izzuddin",middleName:null,surname:"Syakir Ishak",slug:"muhammad-izzuddin-syakir-ishak",fullName:"Muhammad Izzuddin Syakir Ishak"},{id:"324417",title:"Ph.D. Student",name:"Naseem",middleName:null,surname:"Akhtar",slug:"naseem-akhtar",fullName:"Naseem Akhtar"},{id:"328134",title:"Mr.",name:"Mohammad Shaiful",middleName:null,surname:"Yusuff",slug:"mohammad-shaiful-yusuff",fullName:"Mohammad Shaiful Yusuff"},{id:"328135",title:"Mr.",name:"Abdul",middleName:null,surname:"Qadir",slug:"abdul-qadir",fullName:"Abdul Qadir"}]},{id:"73757",title:"Groundwater Recharges Technology for Water Resource Management: A Case Study",slug:"groundwater-recharges-technology-for-water-resource-management-a-case-study",totalDownloads:574,totalCrossrefCites:0,totalDimensionsCites:0,abstract:"The irregularity in monsoon has severely affected the water availability at surface and sub-surface systems. Diminishing surface and sub-surface availability has not only decreased the water availability, but it additionally affected the ecosystem and increased disastrous situations like floods and droughts, resulting problems of stress on groundwater recharge. Groundwater recharge is a technique by which infiltrated water passes through the unsaturated region of groundwater and joins the water table. It is based upon soil type, land use land cover, geomorphology, geophysical and climate (viz. rainfall, temperature, humidity etc.) characteristics of a region. Over the years, due to variations in weather pattern and overexploitation of aquifers groundwater recharge has decreased and groundwater level has reduced in the most parts of the country. This has led to severe water deficit problems in several parts of the country. This can be solved by different direct and indirect methods of groundwater recharge technology. This technology can reduce the wastage of water and enhance groundwater availability for uses in different sector like irrigation, domestic and industrial uses.",book:{id:"9981",slug:"groundwater-management-and-resources",title:"Groundwater Management and Resources",fullTitle:"Groundwater Management and Resources"},signatures:"Jatoth Veeranna and Pawan Jeet",authors:[{id:"325776",title:"Dr.",name:"Pawan",middleName:null,surname:"Jeet",slug:"pawan-jeet",fullName:"Pawan Jeet"},{id:"328200",title:"Mr.",name:"Jatoth",middleName:null,surname:"Veeranna",slug:"jatoth-veeranna",fullName:"Jatoth Veeranna"}]},{id:"62709",title:"Sustainability of Irrigation in Uzbekistan: Implications for Women Farmers",slug:"sustainability-of-irrigation-in-uzbekistan-implications-for-women-farmers",totalDownloads:1039,totalCrossrefCites:1,totalDimensionsCites:2,abstract:"This chapter focuses on a discussion of how global efforts to align local irrigation management with the good governance principles affect the lives of the rural poor, specifically women. Drawing in empirical data collected in post-soviet Uzbekistan, I illuminate unexpected effects of an apparently well-intended irrigation project on those categories of farmers whose connections to state apparatus of agricultural commerce of cotton were weak. Using fieldwork data from a village largely affected by desiccation of Aral Sea, I describe the everyday struggles by these people, who are mostly women, engage to make their living and provide subsistence to their families in situation of economic trauma, environmental disaster, and massive outmigration of male population. This analysis puts forward the local voices of real people whose lives are being restructured by sustainability oriented actions. Such perspective is often missed in scholarly and professional literature. These findings are hoped to assist policy developers in formulating irrigation programs in ways that would embrace sustainability both in terms of environmental and social justice.",book:{id:"6886",slug:"water-and-sustainability",title:"Water and Sustainability",fullTitle:"Water and Sustainability"},signatures:"Elena Kim",authors:null},{id:"71359",title:"Effects of Climate Change on Water Resources, Indices, and Related Activities in Colombia",slug:"effects-of-climate-change-on-water-resources-indices-and-related-activities-in-colombia",totalDownloads:713,totalCrossrefCites:3,totalDimensionsCites:4,abstract:"In Colombia, a country with great climatic diversity, the water balance is affected in one way or another by climate change depending on the region. Thus, there may be increases and decreases in precipitation and, in all cases, a huge increase in temperature. This document presents some studies carried out in different areas of the country regarding the effects of climate change on water resources, including its influence on hydroelectric power generation, some changes in the water balance in arid areas, and the opportunity to ensemble climate change scenarios. Likewise, it outlines a possible future water supply-demand relationship, where supply is associated with a change in the water balance and demand with some crops, activities, and sectors that need water to survive. This allows to estimate some future status indices to see the overall picture of climate change in connection with the country’s water resources.",book:{id:"8098",slug:"resources-of-water",title:"Resources of Water",fullTitle:"Resources of Water"},signatures:"Nathaly Güiza-Villa, Carlos Gay-García and Jesús Efren Ospina-Noreña",authors:[{id:"311362",title:"Ph.D.",name:"Jesús Efren",middleName:null,surname:"Ospina-Noreña",slug:"jesus-efren-ospina-norena",fullName:"Jesús Efren Ospina-Noreña"},{id:"311363",title:"Dr.",name:"Carlos",middleName:null,surname:"Gay-García",slug:"carlos-gay-garcia",fullName:"Carlos Gay-García"},{id:"311364",title:"M.Sc.",name:"Nathaly",middleName:null,surname:"Güiza-Villa",slug:"nathaly-guiza-villa",fullName:"Nathaly Güiza-Villa"}]}],onlineFirstChaptersFilter:{topicId:"872",limit:6,offset:0},onlineFirstChaptersCollection:[],onlineFirstChaptersTotal:0},preDownload:{success:null,errors:{}},subscriptionForm:{success:null,errors:{}},aboutIntechopen:{},privacyPolicy:{},peerReviewing:{},howOpenAccessPublishingWithIntechopenWorks:{},sponsorshipBooks:{sponsorshipBooks:[],offset:8,limit:8,total:0},allSeries:{pteSeriesList:[{id:"14",title:"Artificial Intelligence",numberOfPublishedBooks:9,numberOfPublishedChapters:89,numberOfOpenTopics:6,numberOfUpcomingTopics:0,issn:"2633-1403",doi:"10.5772/intechopen.79920",isOpenForSubmission:!0},{id:"7",title:"Biomedical Engineering",numberOfPublishedBooks:12,numberOfPublishedChapters:104,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2631-5343",doi:"10.5772/intechopen.71985",isOpenForSubmission:!0}],lsSeriesList:[{id:"11",title:"Biochemistry",numberOfPublishedBooks:32,numberOfPublishedChapters:318,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2632-0983",doi:"10.5772/intechopen.72877",isOpenForSubmission:!0},{id:"25",title:"Environmental Sciences",numberOfPublishedBooks:1,numberOfPublishedChapters:12,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2754-6713",doi:"10.5772/intechopen.100362",isOpenForSubmission:!0},{id:"10",title:"Physiology",numberOfPublishedBooks:11,numberOfPublishedChapters:141,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2631-8261",doi:"10.5772/intechopen.72796",isOpenForSubmission:!0}],hsSeriesList:[{id:"3",title:"Dentistry",numberOfPublishedBooks:8,numberOfPublishedChapters:129,numberOfOpenTopics:2,numberOfUpcomingTopics:0,issn:"2631-6218",doi:"10.5772/intechopen.71199",isOpenForSubmission:!0},{id:"6",title:"Infectious Diseases",numberOfPublishedBooks:13,numberOfPublishedChapters:113,numberOfOpenTopics:3,numberOfUpcomingTopics:1,issn:"2631-6188",doi:"10.5772/intechopen.71852",isOpenForSubmission:!0},{id:"13",title:"Veterinary Medicine and Science",numberOfPublishedBooks:11,numberOfPublishedChapters:105,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2632-0517",doi:"10.5772/intechopen.73681",isOpenForSubmission:!0}],sshSeriesList:[{id:"22",title:"Business, Management and Economics",numberOfPublishedBooks:1,numberOfPublishedChapters:19,numberOfOpenTopics:2,numberOfUpcomingTopics:1,issn:"2753-894X",doi:"10.5772/intechopen.100359",isOpenForSubmission:!0},{id:"23",title:"Education and Human Development",numberOfPublishedBooks:0,numberOfPublishedChapters:5,numberOfOpenTopics:1,numberOfUpcomingTopics:1,issn:null,doi:"10.5772/intechopen.100360",isOpenForSubmission:!0},{id:"24",title:"Sustainable Development",numberOfPublishedBooks:0,numberOfPublishedChapters:15,numberOfOpenTopics:5,numberOfUpcomingTopics:0,issn:null,doi:"10.5772/intechopen.100361",isOpenForSubmission:!0}],testimonialsList:[{id:"6",text:"It is great to work with the IntechOpen to produce a worthwhile collection of research that also becomes a great educational resource and guide for future research endeavors.",author:{id:"259298",name:"Edward",surname:"Narayan",institutionString:null,profilePictureURL:"https://mts.intechopen.com/storage/users/259298/images/system/259298.jpeg",slug:"edward-narayan",institution:{id:"3",name:"University of Queensland",country:{id:null,name:"Australia"}}}},{id:"13",text:"The collaboration with and support of the technical staff of IntechOpen is fantastic. The whole process of submitting an article and editing of the submitted article goes extremely smooth and fast, the number of reads and downloads of chapters is high, and the contributions are also frequently cited.",author:{id:"55578",name:"Antonio",surname:"Jurado-Navas",institutionString:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRisIQAS/Profile_Picture_1626166543950",slug:"antonio-jurado-navas",institution:{id:"720",name:"University of Malaga",country:{id:null,name:"Spain"}}}}]},series:{item:{id:"13",title:"Veterinary Medicine and Science",doi:"10.5772/intechopen.73681",issn:"2632-0517",scope:"Paralleling similar advances in the medical field, astounding advances occurred in Veterinary Medicine and Science in recent decades. These advances have helped foster better support for animal health, more humane animal production, and a better understanding of the physiology of endangered species to improve the assisted reproductive technologies or the pathogenesis of certain diseases, where animals can be used as models for human diseases (like cancer, degenerative diseases or fertility), and even as a guarantee of public health. Bridging Human, Animal, and Environmental health, the holistic and integrative “One Health” concept intimately associates the developments within those fields, projecting its advancements into practice. This book series aims to tackle various animal-related medicine and sciences fields, providing thematic volumes consisting of high-quality significant research directed to researchers and postgraduates. It aims to give us a glimpse into the new accomplishments in the Veterinary Medicine and Science field. By addressing hot topics in veterinary sciences, we aim to gather authoritative texts within each issue of this series, providing in-depth overviews and analysis for graduates, academics, and practitioners and foreseeing a deeper understanding of the subject. Forthcoming texts, written and edited by experienced researchers from both industry and academia, will also discuss scientific challenges faced today in Veterinary Medicine and Science. In brief, we hope that books in this series will provide accessible references for those interested or working in this field and encourage learning in a range of different topics.",coverUrl:"https://cdn.intechopen.com/series/covers/13.jpg",latestPublicationDate:"June 17th, 2022",hasOnlineFirst:!0,numberOfPublishedBooks:11,editor:{id:"38652",title:"Prof.",name:"Rita",middleName:null,surname:"Payan-Carreira",slug:"rita-payan-carreira",fullName:"Rita Payan-Carreira",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRiFPQA0/Profile_Picture_1614601496313",biography:"Rita Payan Carreira earned her Veterinary Degree from the Faculty of Veterinary Medicine in Lisbon, Portugal, in 1985. She obtained her Ph.D. in Veterinary Sciences from the University of Trás-os-Montes e Alto Douro, Portugal. After almost 32 years of teaching at the University of Trás-os-Montes and Alto Douro, she recently moved to the University of Évora, Department of Veterinary Medicine, where she teaches in the field of Animal Reproduction and Clinics. Her primary research areas include the molecular markers of the endometrial cycle and the embryo–maternal interaction, including oxidative stress and the reproductive physiology and disorders of sexual development, besides the molecular determinants of male and female fertility. She often supervises students preparing their master's or doctoral theses. She is also a frequent referee for various journals.",institutionString:null,institution:{name:"University of Évora",institutionURL:null,country:{name:"Portugal"}}},editorTwo:null,editorThree:null},subseries:{paginationCount:6,paginationItems:[{id:"19",title:"Animal Science",coverUrl:"https://cdn.intechopen.com/series_topics/covers/19.jpg",editor:{id:"259298",title:"Dr.",name:"Edward",middleName:null,surname:"Narayan",slug:"edward-narayan",fullName:"Edward Narayan",profilePictureURL:"https://mts.intechopen.com/storage/users/259298/images/system/259298.jpeg",biography:"Dr. Edward Narayan graduated with Ph.D. degree in Biology from the University of the South Pacific and pioneered non-invasive reproductive and stress endocrinology tools for amphibians - the novel development and validation of non-invasive enzyme immunoassays for the evaluation of reproductive hormonal cycle and stress hormone responses to environmental stressors. \nDr. Narayan leads the Stress Lab (Comparative Physiology and Endocrinology) at the University of Queensland. A dynamic career research platform which is based on the thematic areas of comparative vertebrate physiology, stress endocrinology, reproductive endocrinology, animal health and welfare, and conservation biology. \nEdward has supervised 40 research students and published over 60 peer reviewed research.",institutionString:null,institution:{name:"University of Queensland",institutionURL:null,country:{name:"Australia"}}},editorTwo:null,editorThree:null,editorialBoard:[{id:"258334",title:"Dr.",name:"Carlos Eduardo",middleName:null,surname:"Fonseca-Alves",slug:"carlos-eduardo-fonseca-alves",fullName:"Carlos Eduardo Fonseca-Alves",profilePictureURL:"https://mts.intechopen.com/storage/users/258334/images/system/258334.jpg",institutionString:null,institution:{name:"Universidade Paulista",institutionURL:null,country:{name:"Brazil"}}},{id:"191123",title:"Dr.",name:"Juan José",middleName:null,surname:"Valdez-Alarcón",slug:"juan-jose-valdez-alarcon",fullName:"Juan José Valdez-Alarcón",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bSBfcQAG/Profile_Picture_1631354558068",institutionString:"Universidad Michoacana de San Nicolás de Hidalgo",institution:{name:"Universidad Michoacana de San Nicolás de Hidalgo",institutionURL:null,country:{name:"Mexico"}}},{id:"161556",title:"Dr.",name:"Maria Dos Anjos",middleName:null,surname:"Pires",slug:"maria-dos-anjos-pires",fullName:"Maria Dos Anjos Pires",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bS8q2QAC/Profile_Picture_1633432838418",institutionString:null,institution:{name:"University of Trás-os-Montes and Alto Douro",institutionURL:null,country:{name:"Portugal"}}},{id:"209839",title:"Dr.",name:"Marina",middleName:null,surname:"Spinu",slug:"marina-spinu",fullName:"Marina Spinu",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRLXpQAO/Profile_Picture_1630044895475",institutionString:null,institution:{name:"University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca",institutionURL:null,country:{name:"Romania"}}},{id:"92185",title:"Dr.",name:"Sara",middleName:null,surname:"Savic",slug:"sara-savic",fullName:"Sara Savic",profilePictureURL:"https://mts.intechopen.com/storage/users/92185/images/system/92185.jfif",institutionString:'Scientific Veterinary Institute "Novi Sad"',institution:{name:'Scientific Veterinary Institute "Novi Sad"',institutionURL:null,country:{name:"Serbia"}}}]},{id:"20",title:"Animal Nutrition",coverUrl:"https://cdn.intechopen.com/series_topics/covers/20.jpg",editor:{id:"175967",title:"Dr.",name:"Manuel",middleName:null,surname:"Gonzalez Ronquillo",slug:"manuel-gonzalez-ronquillo",fullName:"Manuel Gonzalez Ronquillo",profilePictureURL:"https://mts.intechopen.com/storage/users/175967/images/system/175967.png",biography:"Dr. Manuel González Ronquillo obtained his doctorate degree from the University of Zaragoza, Spain, in 2001. He is a research professor at the Faculty of Veterinary Medicine and Animal Husbandry, Autonomous University of the State of Mexico. He is also a level-2 researcher. He received a Fulbright-Garcia Robles fellowship for a postdoctoral stay at the US Dairy Forage Research Center, Madison, Wisconsin, USA in 2008–2009. He received grants from Alianza del Pacifico for a stay at the University of Magallanes, Chile, in 2014, and from Consejo Nacional de Ciencia y Tecnología (CONACyT) to work in the Food and Agriculture Organization’s Animal Production and Health Division (AGA), Rome, Italy, in 2014–2015. He has collaborated with researchers from different countries and published ninety-eight journal articles. He teaches various degree courses in zootechnics, sheep production, and agricultural sciences and natural resources.\n\nDr. Ronquillo’s research focuses on the evaluation of sustainable animal diets (StAnD), using native resources of the region, decreasing carbon footprint, and applying meta-analysis and mathematical models for a better understanding of animal production.",institutionString:null,institution:{name:"Universidad Autónoma del Estado de México",institutionURL:null,country:{name:"Mexico"}}},editorTwo:null,editorThree:null,editorialBoard:[{id:"175762",title:"Dr.",name:"Alfredo J.",middleName:null,surname:"Escribano",slug:"alfredo-j.-escribano",fullName:"Alfredo J. Escribano",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRGnzQAG/Profile_Picture_1633076636544",institutionString:"Consultant and Independent Researcher in Industry Sector, Spain",institution:null},{id:"310962",title:"Dr.",name:"Amlan",middleName:"Kumar",surname:"Patra",slug:"amlan-patra",fullName:"Amlan Patra",profilePictureURL:"https://mts.intechopen.com/storage/users/310962/images/system/310962.jpg",institutionString:null,institution:{name:"West Bengal University of Animal and Fishery Sciences",institutionURL:null,country:{name:"India"}}},{id:"216995",title:"Prof.",name:"Figen",middleName:null,surname:"Kırkpınar",slug:"figen-kirkpinar",fullName:"Figen Kırkpınar",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRMzxQAG/Profile_Picture_1625722918145",institutionString:null,institution:{name:"Ege University",institutionURL:null,country:{name:"Turkey"}}}]},{id:"28",title:"Animal Reproductive Biology and Technology",coverUrl:"https://cdn.intechopen.com/series_topics/covers/28.jpg",editor:{id:"177225",title:"Prof.",name:"Rosa Maria Lino Neto",middleName:null,surname:"Pereira",slug:"rosa-maria-lino-neto-pereira",fullName:"Rosa Maria Lino Neto Pereira",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bS9wkQAC/Profile_Picture_1624519982291",biography:"Rosa Maria Lino Neto Pereira (DVM, MsC, PhD and) is currently a researcher at the Genetic Resources and Biotechnology Unit of the National Institute of Agrarian and Veterinarian Research (INIAV, Portugal). She is the head of the Reproduction and Embryology Laboratories and was lecturer of Reproduction and Reproductive Biotechnologies at Veterinary Medicine Faculty. She has over 25 years of experience working in reproductive biology and biotechnology areas with a special emphasis on embryo and gamete cryopreservation, for research and animal genetic resources conservation, leading research projects with several peer-reviewed papers. Rosa Pereira is member of the ERFP-FAO Ex situ Working Group and of the Management Commission of the Portuguese Animal Germplasm Bank.",institutionString:"The National Institute for Agricultural and Veterinary Research. Portugal",institution:null},editorTwo:null,editorThree:null,editorialBoard:[{id:"90066",title:"Dr.",name:"Alexandre",middleName:"Rodrigues",surname:"Silva",slug:"alexandre-silva",fullName:"Alexandre Silva",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRt8pQAC/Profile_Picture_1622531020756",institutionString:null,institution:{name:"Universidade Federal Rural do Semi-Árido",institutionURL:null,country:{name:"Brazil"}}},{id:"176987",title:"Ph.D.",name:"María-José",middleName:"Carrascosa",surname:"Argente",slug:"maria-jose-argente",fullName:"María-José Argente",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bS9vOQAS/Profile_Picture_1630330499537",institutionString:null,institution:{name:"Miguel Hernandez University",institutionURL:null,country:{name:"Spain"}}},{id:"321396",title:"Prof.",name:"Muhammad Subhan",middleName:null,surname:"Qureshi",slug:"muhammad-subhan-qureshi",fullName:"Muhammad Subhan Qureshi",profilePictureURL:"https://mts.intechopen.com/storage/users/321396/images/system/321396.jpg",institutionString:null,institution:{name:"University of Agriculture",institutionURL:null,country:{name:"Pakistan"}}},{id:"183723",title:"Dr.",name:"Xiaojun",middleName:null,surname:"Liu",slug:"xiaojun-liu",fullName:"Xiaojun Liu",profilePictureURL:"https://mts.intechopen.com/storage/users/183723/images/system/183723.jpg",institutionString:null,institution:null}]}]},overviewPageOFChapters:{paginationCount:10,paginationItems:[{id:"82380",title:"Evolution of Parasitism and Pathogenic Adaptations in Certain Medically Important Fungi",doi:"10.5772/intechopen.105206",signatures:"Gokul Shankar Sabesan, Ranjit Singh AJA, Ranjith Mehenderkar and Basanta Kumar Mohanty",slug:"evolution-of-parasitism-and-pathogenic-adaptations-in-certain-medically-important-fungi",totalDownloads:3,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Fungal Infectious Diseases - Annual Volume 2022",coverURL:"https://cdn.intechopen.com/books/images_new/11400.jpg",subseries:{id:"4",title:"Fungal Infectious Diseases"}}},{id:"82367",title:"Spatial Variation and Factors Associated with Unsuppressed HIV Viral Load among Women in an HIV Hyperendemic Area of KwaZulu-Natal, South Africa",doi:"10.5772/intechopen.105547",signatures:"Adenike O. Soogun, Ayesha B.M. Kharsany, Temesgen Zewotir and Delia North",slug:"spatial-variation-and-factors-associated-with-unsuppressed-hiv-viral-load-among-women-in-an-hiv-hype",totalDownloads:9,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"HIV-AIDS - Updates, Perspectives and Applications",coverURL:"https://cdn.intechopen.com/books/images_new/11575.jpg",subseries:{id:"6",title:"Viral Infectious Diseases"}}},{id:"82193",title:"Enterococcal Infections: Recent Nomenclature and emerging trends",doi:"10.5772/intechopen.104792",signatures:"Kavita Raja",slug:"enterococcal-infections-recent-nomenclature-and-emerging-trends",totalDownloads:6,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Streptococcal Infections",coverURL:"https://cdn.intechopen.com/books/images_new/10828.jpg",subseries:{id:"3",title:"Bacterial Infectious Diseases"}}},{id:"82207",title:"Management Strategies in Perinatal HIV",doi:"10.5772/intechopen.105451",signatures:"Kayla Aleshire and Rima Bazzi",slug:"management-strategies-in-perinatal-hiv",totalDownloads:7,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"HIV-AIDS - Updates, Perspectives and Applications",coverURL:"https://cdn.intechopen.com/books/images_new/11575.jpg",subseries:{id:"6",title:"Viral Infectious Diseases"}}}]},overviewPagePublishedBooks:{paginationCount:13,paginationItems:[{type:"book",id:"6667",title:"Influenza",subtitle:"Therapeutics and Challenges",coverURL:"https://cdn.intechopen.com/books/images_new/6667.jpg",slug:"influenza-therapeutics-and-challenges",publishedDate:"September 19th 2018",editedByType:"Edited by",bookSignature:"Shailendra K. Saxena",hash:"105e347b2d5dbbe6b593aceffa051efa",volumeInSeries:1,fullTitle:"Influenza - Therapeutics and Challenges",editors:[{id:"158026",title:"Prof.",name:"Shailendra K.",middleName:null,surname:"Saxena",slug:"shailendra-k.-saxena",fullName:"Shailendra K. Saxena",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRET3QAO/Profile_Picture_2022-05-10T10:10:26.jpeg",biography:"Professor Dr. Shailendra K. Saxena is a vice dean and professor at King George's Medical University, Lucknow, India. His research interests involve understanding the molecular mechanisms of host defense during human viral infections and developing new predictive, preventive, and therapeutic strategies for them using Japanese encephalitis virus (JEV), HIV, and emerging viruses as a model via stem cell and cell culture technologies. His research work has been published in various high-impact factor journals (Science, PNAS, Nature Medicine) with a high number of citations. He has received many awards and honors in India and abroad including various Young Scientist Awards, BBSRC India Partnering Award, and Dr. JC Bose National Award of Department of Biotechnology, Min. of Science and Technology, Govt. of India. Dr. Saxena is a fellow of various international societies/academies including the Royal College of Pathologists, United Kingdom; Royal Society of Medicine, London; Royal Society of Biology, United Kingdom; Royal Society of Chemistry, London; and Academy of Translational Medicine Professionals, Austria. He was named a Global Leader in Science by The Scientist. He is also an international opinion leader/expert in vaccination for Japanese encephalitis by IPIC (UK).",institutionString:"King George's Medical University",institution:{name:"King George's Medical University",institutionURL:null,country:{name:"India"}}}]},{type:"book",id:"7064",title:"Current Perspectives in Human Papillomavirus",subtitle:null,coverURL:"https://cdn.intechopen.com/books/images_new/7064.jpg",slug:"current-perspectives-in-human-papillomavirus",publishedDate:"May 2nd 2019",editedByType:"Edited by",bookSignature:"Shailendra K. Saxena",hash:"d92a4085627bab25ddc7942fbf44cf05",volumeInSeries:2,fullTitle:"Current Perspectives in Human Papillomavirus",editors:[{id:"158026",title:"Prof.",name:"Shailendra K.",middleName:null,surname:"Saxena",slug:"shailendra-k.-saxena",fullName:"Shailendra K. Saxena",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRET3QAO/Profile_Picture_2022-05-10T10:10:26.jpeg",biography:"Professor Dr. Shailendra K. Saxena is a vice dean and professor at King George's Medical University, Lucknow, India. His research interests involve understanding the molecular mechanisms of host defense during human viral infections and developing new predictive, preventive, and therapeutic strategies for them using Japanese encephalitis virus (JEV), HIV, and emerging viruses as a model via stem cell and cell culture technologies. His research work has been published in various high-impact factor journals (Science, PNAS, Nature Medicine) with a high number of citations. He has received many awards and honors in India and abroad including various Young Scientist Awards, BBSRC India Partnering Award, and Dr. JC Bose National Award of Department of Biotechnology, Min. of Science and Technology, Govt. of India. Dr. Saxena is a fellow of various international societies/academies including the Royal College of Pathologists, United Kingdom; Royal Society of Medicine, London; Royal Society of Biology, United Kingdom; Royal Society of Chemistry, London; and Academy of Translational Medicine Professionals, Austria. He was named a Global Leader in Science by The Scientist. He is also an international opinion leader/expert in vaccination for Japanese encephalitis by IPIC (UK).",institutionString:"King George's Medical University",institution:{name:"King George's Medical University",institutionURL:null,country:{name:"India"}}}]},{type:"book",id:"7123",title:"Current Topics in Neglected Tropical Diseases",subtitle:null,coverURL:"https://cdn.intechopen.com/books/images_new/7123.jpg",slug:"current-topics-in-neglected-tropical-diseases",publishedDate:"December 4th 2019",editedByType:"Edited by",bookSignature:"Alfonso J. Rodriguez-Morales",hash:"61c627da05b2ace83056d11357bdf361",volumeInSeries:3,fullTitle:"Current Topics in Neglected Tropical Diseases",editors:[{id:"131400",title:"Prof.",name:"Alfonso J.",middleName:null,surname:"Rodriguez-Morales",slug:"alfonso-j.-rodriguez-morales",fullName:"Alfonso J. Rodriguez-Morales",profilePictureURL:"https://mts.intechopen.com/storage/users/131400/images/system/131400.png",biography:"Dr. Rodriguez-Morales is an expert in tropical and emerging diseases, particularly zoonotic and vector-borne diseases (especially arboviral diseases). He is the president of the Travel Medicine Committee of the Pan-American Infectious Diseases Association (API), as well as the president of the Colombian Association of Infectious Diseases (ACIN). He is a member of the Committee on Tropical Medicine, Zoonoses, and Travel Medicine of ACIN. He is a vice-president of the Latin American Society for Travel Medicine (SLAMVI) and a Member of the Council of the International Society for Infectious Diseases (ISID). Since 2014, he has been recognized as a Senior Researcher, at the Ministry of Science of Colombia. He is a professor at the Faculty of Medicine of the Fundacion Universitaria Autonoma de las Americas, in Pereira, Risaralda, Colombia. He is an External Professor, Master in Research on Tropical Medicine and International Health, Universitat de Barcelona, Spain. He is also a professor at the Master in Clinical Epidemiology and Biostatistics, Universidad Científica del Sur, Lima, Peru. In 2021 he has been awarded the “Raul Isturiz Award” Medal of the API. Also, in 2021, he was awarded with the “Jose Felix Patiño” Asclepius Staff Medal of the Colombian Medical College, due to his scientific contributions to COVID-19 during the pandemic. He is currently the Editor in Chief of the journal Travel Medicine and Infectious Diseases. His Scopus H index is 47 (Google Scholar H index, 68).",institutionString:"Institución Universitaria Visión de las Américas, Colombia",institution:null}]},{type:"book",id:"7839",title:"Malaria",subtitle:null,coverURL:"https://cdn.intechopen.com/books/images_new/7839.jpg",slug:"malaria",publishedDate:"December 11th 2019",editedByType:"Edited by",bookSignature:"Fyson H. Kasenga",hash:"91cde4582ead884cb0f355a19b67cd56",volumeInSeries:4,fullTitle:"Malaria",editors:[{id:"86725",title:"Dr.",name:"Fyson",middleName:"Hanania",surname:"Kasenga",slug:"fyson-kasenga",fullName:"Fyson Kasenga",profilePictureURL:"https://mts.intechopen.com/storage/users/86725/images/system/86725.jpg",biography:"Dr. Kasenga is a graduate of Tumaini University, Kilimanjaro Christian Medical College, Moshi, Tanzania and Umeå University, Sweden. He obtained a Master’s degree in Public Health and PhD in Public Health and Epidemiology. He has a background in Clinical Medicine and has taken courses at higher diploma levels in public health from University of Transkei, Republic of South Africa, and African Medical and Research Foundation (AMREF) in Nairobi, Kenya. Dr. Kasenga worked in different places in and outside Malawi, and has held various positions, such as Licensed Medical Officer, HIV/AIDS Programme Officer, HIV/AIDS resource person in the International Department of Diakonhjemet College, Oslo, Norway. He also managed an Integrated HIV/AIDS Prevention programme for over 5 years. He is currently working as a Director for the Health Ministries Department of Malawi Union of the Seventh Day Adventist Church. Dr. Kasenga has published over 5 articles on HIV/AIDS issues focusing on Prevention of Mother to Child Transmission of HIV (PMTCT), including a book chapter on HIV testing counseling (currently in press). Dr. Kasenga is married to Grace and blessed with three children, a son and two daughters: Happy, Lettice and Sungani.",institutionString:"Malawi Adventist University",institution:{name:"Malawi Adventist University",institutionURL:null,country:{name:"Malawi"}}}]}]},openForSubmissionBooks:{},onlineFirstChapters:{paginationCount:23,paginationItems:[{id:"82392",title:"Nanomaterials as Novel Biomarkers for Cancer Nanotheranostics: State of the Art",doi:"10.5772/intechopen.105700",signatures:"Hao Yu, Zhihai Han, Cunrong Chen and Leisheng Zhang",slug:"nanomaterials-as-novel-biomarkers-for-cancer-nanotheranostics-state-of-the-art",totalDownloads:2,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Biotechnology - Biosensors, Biomaterials and Tissue Engineering - Annual Volume 2022",coverURL:"https://cdn.intechopen.com/books/images_new/11405.jpg",subseries:{id:"9",title:"Biotechnology - Biosensors, Biomaterials and Tissue Engineering"}}},{id:"82184",title:"Biological Sensing Using Infrared SPR Devices Based on ZnO",doi:"10.5772/intechopen.104562",signatures:"Hiroaki Matsui",slug:"biological-sensing-using-infrared-spr-devices-based-on-zno",totalDownloads:4,totalCrossrefCites:0,totalDimensionsCites:0,authors:[{name:"Hiroaki",surname:"Matsui"}],book:{title:"Biosignal Processing",coverURL:"https://cdn.intechopen.com/books/images_new/11153.jpg",subseries:{id:"7",title:"Bioinformatics and Medical Informatics"}}},{id:"82122",title:"Recent Advances in Biosensing in Tissue Engineering and Regenerative Medicine",doi:"10.5772/intechopen.104922",signatures:"Alma T. Banigo, Chigozie A. Nnadiekwe and Emmanuel M. Beasi",slug:"recent-advances-in-biosensing-in-tissue-engineering-and-regenerative-medicine",totalDownloads:13,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Biosignal Processing",coverURL:"https://cdn.intechopen.com/books/images_new/11153.jpg",subseries:{id:"7",title:"Bioinformatics and Medical Informatics"}}},{id:"82080",title:"The Clinical Usefulness of Prostate Cancer Biomarkers: Current and Future Directions",doi:"10.5772/intechopen.103172",signatures:"Donovan McGrowder, Lennox Anderson-Jackson, Lowell Dilworth, Shada Mohansingh, Melisa Anderson Cross, Sophia Bryan, Fabian Miller, Cameil Wilson-Clarke, Chukwuemeka Nwokocha, Ruby Alexander-Lindo and Shelly McFarlane",slug:"the-clinical-usefulness-of-prostate-cancer-biomarkers-current-and-future-directions",totalDownloads:14,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Cancer Bioinformatics",coverURL:"https://cdn.intechopen.com/books/images_new/10661.jpg",subseries:{id:"7",title:"Bioinformatics and Medical Informatics"}}},{id:"82005",title:"Non-Invasive Approach for Glucose Detection in Urine Quality using Its Image Analysis",doi:"10.5772/intechopen.104791",signatures:"Anton Yudhana, Liya Yusrina Sabila, Arsyad Cahya Subrata, Hendriana Helda Pratama and Muhammad Syahrul Akbar",slug:"non-invasive-approach-for-glucose-detection-in-urine-quality-using-its-image-analysis",totalDownloads:4,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Biosignal Processing",coverURL:"https://cdn.intechopen.com/books/images_new/11153.jpg",subseries:{id:"7",title:"Bioinformatics and Medical Informatics"}}},{id:"81778",title:"Influence of Mechanical Properties of Biomaterials on the Reconstruction of Biomedical Parts via Additive Manufacturing Techniques: An Overview",doi:"10.5772/intechopen.104465",signatures:"Babatunde Olamide Omiyale, Akeem Abiodun Rasheed, Robinson Omoboyode Akinnusi and Temitope Olumide Olugbade",slug:"influence-of-mechanical-properties-of-biomaterials-on-the-reconstruction-of-biomedical-parts-via-add",totalDownloads:9,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Biotechnology - Biosensors, Biomaterials and Tissue Engineering - Annual Volume 2022",coverURL:"https://cdn.intechopen.com/books/images_new/11405.jpg",subseries:{id:"9",title:"Biotechnology - Biosensors, Biomaterials and Tissue Engineering"}}},{id:"81751",title:"NanoBioSensors: From Electrochemical Sensors Improvement to Theranostic Applications",doi:"10.5772/intechopen.102552",signatures:"Anielle C.A. Silva, Eliete A. Alvin, Lais S. de Jesus, Caio C.L. de França, Marílya P.G. da Silva, Samaysa L. Lins, Diógenes Meneses, Marcela R. Lemes, Rhanoica O. Guerra, Marcos V. da Silva, Carlo J.F. de Oliveira, Virmondes Rodrigues Junior, Renata M. Etchebehere, Fabiane C. de Abreu, Bruno G. Lucca, Sanívia A.L. Pereira, Rodrigo C. Rosa and Noelio O. Dantas",slug:"nanobiosensors-from-electrochemical-sensors-improvement-to-theranostic-applications",totalDownloads:12,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Biosignal Processing",coverURL:"https://cdn.intechopen.com/books/images_new/11153.jpg",subseries:{id:"7",title:"Bioinformatics and Medical Informatics"}}},{id:"81766",title:"Evolution of Organoids in Oncology",doi:"10.5772/intechopen.104251",signatures:"Allen Thayakumar Basanthakumar, Janitha Chandrasekhar Darlybai and Jyothsna Ganesh",slug:"evolution-of-organoids-in-oncology",totalDownloads:14,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Organoids",coverURL:"https://cdn.intechopen.com/books/images_new/11430.jpg",subseries:null}},{id:"81678",title:"Developmental Studies on Practical Enzymatic Phosphate Ion Biosensors and Microbial BOD Biosensors, and New Insights into the Future Perspectives of These Biosensor Fields",doi:"10.5772/intechopen.104377",signatures:"Hideaki Nakamura",slug:"developmental-studies-on-practical-enzymatic-phosphate-ion-biosensors-and-microbial-bod-biosensors-a",totalDownloads:5,totalCrossrefCites:0,totalDimensionsCites:0,authors:[{name:"Hideaki",surname:"Nakamura"}],book:{title:"Biosignal Processing",coverURL:"https://cdn.intechopen.com/books/images_new/11153.jpg",subseries:{id:"7",title:"Bioinformatics and Medical Informatics"}}},{id:"81547",title:"Organoids and Commercialization",doi:"10.5772/intechopen.104706",signatures:"Anubhab Mukherjee, Aprajita Sinha, Maheshree Maibam, Bharti Bisht and Manash K. Paul",slug:"organoids-and-commercialization",totalDownloads:51,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Organoids",coverURL:"https://cdn.intechopen.com/books/images_new/11430.jpg",subseries:null}}]},subseriesFiltersForOFChapters:[{caption:"Biotechnology - Biosensors, Biomaterials and Tissue Engineering",value:9,count:2,group:"subseries"},{caption:"Bioinformatics and Medical Informatics",value:7,count:17,group:"subseries"}],publishedBooks:{paginationCount:13,paginationItems:[{type:"book",id:"7102",title:"Pneumonia",subtitle:null,coverURL:"https://cdn.intechopen.com/books/images_new/7102.jpg",slug:"pneumonia",publishedDate:"May 11th 2022",editedByType:"Edited by",bookSignature:"Nima Rezaei",hash:"9fd70142814192dcec58a176749f1b60",volumeInSeries:13,fullTitle:"Pneumonia",editors:[{id:"116250",title:"Dr.",name:"Nima",middleName:null,surname:"Rezaei",slug:"nima-rezaei",fullName:"Nima Rezaei",profilePictureURL:"https://mts.intechopen.com/storage/users/116250/images/system/116250.jpg",institutionString:"Tehran University of Medical Sciences",institution:{name:"Tehran University of Medical Sciences",institutionURL:null,country:{name:"Iran"}}}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null},{type:"book",id:"9615",title:"Chikungunya Virus",subtitle:"A Growing Global Public Health Threat",coverURL:"https://cdn.intechopen.com/books/images_new/9615.jpg",slug:"chikungunya-virus-a-growing-global-public-health-threat",publishedDate:"February 9th 2022",editedByType:"Edited by",bookSignature:"Jean Engohang-Ndong",hash:"c960d94a63867dd12a8ab15176a3ff06",volumeInSeries:12,fullTitle:"Chikungunya Virus - A Growing Global Public Health Threat",editors:[{id:"180733",title:"Dr.",name:"Jean",middleName:null,surname:"Engohang-Ndong",slug:"jean-engohang-ndong",fullName:"Jean Engohang-Ndong",profilePictureURL:"https://mts.intechopen.com/storage/users/180733/images/system/180733.png",institutionString:"Kent State University",institution:{name:"Kent State University",institutionURL:null,country:{name:"United States of America"}}}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null},{type:"book",id:"9619",title:"Epstein-Barr Virus",subtitle:"New Trends",coverURL:"https://cdn.intechopen.com/books/images_new/9619.jpg",slug:"epstein-barr-virus-new-trends",publishedDate:"December 22nd 2021",editedByType:"Edited by",bookSignature:"Emmanuel Drouet",hash:"a2128c53becb6064589570cbe8d976f8",volumeInSeries:11,fullTitle:"Epstein-Barr Virus - New Trends",editors:[{id:"188773",title:"Prof.",name:"Emmanuel",middleName:null,surname:"Drouet",slug:"emmanuel-drouet",fullName:"Emmanuel Drouet",profilePictureURL:"https://mts.intechopen.com/storage/users/188773/images/system/188773.png",institutionString:null,institution:{name:"Grenoble Alpes University",institutionURL:null,country:{name:"France"}}}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null},{type:"book",id:"9525",title:"Insights Into Drug Resistance in Staphylococcus aureus",subtitle:null,coverURL:"https://cdn.intechopen.com/books/images_new/9525.jpg",slug:"insights-into-drug-resistance-in-staphylococcus-aureus",publishedDate:"December 8th 2021",editedByType:"Edited by",bookSignature:"Amjad Aqib",hash:"98bb6c1ddb067da67185c272f81c0a27",volumeInSeries:10,fullTitle:"Insights Into Drug Resistance in Staphylococcus aureus",editors:[{id:"229220",title:"Dr.",name:"Amjad",middleName:"Islam",surname:"Aqib",slug:"amjad-aqib",fullName:"Amjad Aqib",profilePictureURL:"https://mts.intechopen.com/storage/users/229220/images/system/229220.png",institutionString:"Cholistan University of Veterinary and Animal Sciences",institution:null}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null},{type:"book",id:"9614",title:"Advances in Candida albicans",subtitle:null,coverURL:"https://cdn.intechopen.com/books/images_new/9614.jpg",slug:"advances-in-candida-albicans",publishedDate:"November 17th 2021",editedByType:"Edited by",bookSignature:"Xinhui Wang",hash:"31d6882518ca749b12715266eed0a018",volumeInSeries:9,fullTitle:"Advances in Candida albicans",editors:[{id:"296531",title:"Dr.",name:"Xinhui",middleName:null,surname:"Wang",slug:"xinhui-wang",fullName:"Xinhui Wang",profilePictureURL:"https://mts.intechopen.com/storage/users/296531/images/system/296531.jpg",institutionString:"Qinghai Normal University",institution:{name:"University of Luxembourg",institutionURL:null,country:{name:"Luxembourg"}}}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null},{type:"book",id:"9528",title:"Current Topics and Emerging Issues in Malaria Elimination",subtitle:null,coverURL:"https://cdn.intechopen.com/books/images_new/9528.jpg",slug:"current-topics-and-emerging-issues-in-malaria-elimination",publishedDate:"July 21st 2021",editedByType:"Edited by",bookSignature:"Alfonso J. Rodriguez-Morales",hash:"7f178329cc42e691efe226b32f14e2ea",volumeInSeries:8,fullTitle:"Current Topics and Emerging Issues in Malaria Elimination",editors:[{id:"131400",title:"Prof.",name:"Alfonso J.",middleName:null,surname:"Rodriguez-Morales",slug:"alfonso-j.-rodriguez-morales",fullName:"Alfonso J. Rodriguez-Morales",profilePictureURL:"https://mts.intechopen.com/storage/users/131400/images/system/131400.png",institutionString:"Institución Universitaria Visión de las Américas, Colombia",institution:null}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null},{type:"book",id:"9613",title:"Dengue Fever in a One Health Perspective",subtitle:null,coverURL:"https://cdn.intechopen.com/books/images_new/9613.jpg",slug:"dengue-fever-in-a-one-health-perspective",publishedDate:"October 28th 2020",editedByType:"Edited by",bookSignature:"Márcia Aparecida Sperança",hash:"77ecce8195c11092230b4156df6d83ff",volumeInSeries:7,fullTitle:"Dengue Fever in a One Health Perspective",editors:[{id:"176579",title:"Dr.",name:"Márcia Aparecida",middleName:null,surname:"Sperança",slug:"marcia-aparecida-speranca",fullName:"Márcia Aparecida Sperança",profilePictureURL:"https://mts.intechopen.com/storage/users/176579/images/system/176579.jpg",institutionString:null,institution:{name:"Universidade Federal do ABC",institutionURL:null,country:{name:"Brazil"}}}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null},{type:"book",id:"7981",title:"Overview on Echinococcosis",subtitle:null,coverURL:"https://cdn.intechopen.com/books/images_new/7981.jpg",slug:"overview-on-echinococcosis",publishedDate:"April 22nd 2020",editedByType:"Edited by",bookSignature:"Fethi Derbel and Meriem Braiki",hash:"24dee9209f3fd6b7cd28f042da0076f0",volumeInSeries:6,fullTitle:"Overview on Echinococcosis",editors:[{id:"62900",title:"Prof.",name:"Fethi",middleName:null,surname:"Derbel",slug:"fethi-derbel",fullName:"Fethi Derbel",profilePictureURL:"https://mts.intechopen.com/storage/users/62900/images/system/62900.jpeg",institutionString:"Clinique les Oliviers",institution:null}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null},{type:"book",id:"7887",title:"Hepatitis B and C",subtitle:null,coverURL:"https://cdn.intechopen.com/books/images_new/7887.jpg",slug:"hepatitis-b-and-c",publishedDate:"April 8th 2020",editedByType:"Edited by",bookSignature:"Luis Rodrigo",hash:"8dd6dab483cf505d83caddaeaf497f2c",volumeInSeries:5,fullTitle:"Hepatitis B and C",editors:[{id:"73208",title:"Prof.",name:"Luis",middleName:null,surname:"Rodrigo",slug:"luis-rodrigo",fullName:"Luis Rodrigo",profilePictureURL:"https://mts.intechopen.com/storage/users/73208/images/system/73208.jpg",institutionString:"University of Oviedo",institution:{name:"University of Oviedo",institutionURL:null,country:{name:"Spain"}}}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null},{type:"book",id:"7839",title:"Malaria",subtitle:null,coverURL:"https://cdn.intechopen.com/books/images_new/7839.jpg",slug:"malaria",publishedDate:"December 11th 2019",editedByType:"Edited by",bookSignature:"Fyson H. Kasenga",hash:"91cde4582ead884cb0f355a19b67cd56",volumeInSeries:4,fullTitle:"Malaria",editors:[{id:"86725",title:"Dr.",name:"Fyson",middleName:"Hanania",surname:"Kasenga",slug:"fyson-kasenga",fullName:"Fyson Kasenga",profilePictureURL:"https://mts.intechopen.com/storage/users/86725/images/system/86725.jpg",institutionString:"Malawi Adventist University",institution:{name:"Malawi Adventist University",institutionURL:null,country:{name:"Malawi"}}}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null},{type:"book",id:"7123",title:"Current Topics in Neglected Tropical Diseases",subtitle:null,coverURL:"https://cdn.intechopen.com/books/images_new/7123.jpg",slug:"current-topics-in-neglected-tropical-diseases",publishedDate:"December 4th 2019",editedByType:"Edited by",bookSignature:"Alfonso J. Rodriguez-Morales",hash:"61c627da05b2ace83056d11357bdf361",volumeInSeries:3,fullTitle:"Current Topics in Neglected Tropical Diseases",editors:[{id:"131400",title:"Prof.",name:"Alfonso J.",middleName:null,surname:"Rodriguez-Morales",slug:"alfonso-j.-rodriguez-morales",fullName:"Alfonso J. Rodriguez-Morales",profilePictureURL:"https://mts.intechopen.com/storage/users/131400/images/system/131400.png",institutionString:"Institución Universitaria Visión de las Américas, Colombia",institution:null}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null},{type:"book",id:"7064",title:"Current Perspectives in Human Papillomavirus",subtitle:null,coverURL:"https://cdn.intechopen.com/books/images_new/7064.jpg",slug:"current-perspectives-in-human-papillomavirus",publishedDate:"May 2nd 2019",editedByType:"Edited by",bookSignature:"Shailendra K. Saxena",hash:"d92a4085627bab25ddc7942fbf44cf05",volumeInSeries:2,fullTitle:"Current Perspectives in Human Papillomavirus",editors:[{id:"158026",title:"Prof.",name:"Shailendra K.",middleName:null,surname:"Saxena",slug:"shailendra-k.-saxena",fullName:"Shailendra K. Saxena",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRET3QAO/Profile_Picture_2022-05-10T10:10:26.jpeg",institutionString:"King George's Medical University",institution:{name:"King George's Medical University",institutionURL:null,country:{name:"India"}}}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null}]},subseriesFiltersForPublishedBooks:[{group:"subseries",caption:"Bacterial Infectious Diseases",value:3,count:2},{group:"subseries",caption:"Parasitic Infectious Diseases",value:5,count:4},{group:"subseries",caption:"Viral Infectious Diseases",value:6,count:7}],publicationYearFilters:[{group:"publicationYear",caption:"2022",value:2022,count:2},{group:"publicationYear",caption:"2021",value:2021,count:4},{group:"publicationYear",caption:"2020",value:2020,count:3},{group:"publicationYear",caption:"2019",value:2019,count:3},{group:"publicationYear",caption:"2018",value:2018,count:1}],authors:{paginationCount:229,paginationItems:[{id:"318170",title:"Dr.",name:"Aneesa",middleName:null,surname:"Moolla",slug:"aneesa-moolla",fullName:"Aneesa Moolla",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/318170/images/system/318170.png",biography:"Dr. Aneesa Moolla has extensive experience in the diverse fields of health care having previously worked in dental private practice, at the Red Cross Flying Doctors association, and in healthcare corporate settings. She is now a lecturer at the University of Witwatersrand, South Africa, and a principal researcher at the Health Economics and Epidemiology Research Office (HE2RO), South Africa. Dr. Moolla holds a Ph.D. in Psychology with her research being focused on mental health and resilience. In her professional work capacity, her research has further expanded into the fields of early childhood development, mental health, the HIV and TB care cascades, as well as COVID. She is also a UNESCO-trained International Bioethics Facilitator.",institutionString:"University of the Witwatersrand",institution:{name:"University of the Witwatersrand",country:{name:"South Africa"}}},{id:"419588",title:"Ph.D.",name:"Sergio",middleName:"Alexandre",surname:"Gehrke",slug:"sergio-gehrke",fullName:"Sergio Gehrke",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y000038WgMKQA0/Profile_Picture_2022-06-02T11:44:20.jpg",biography:"Dr. Sergio Alexandre Gehrke is a doctorate holder in two fields. The first is a Ph.D. in Cellular and Molecular Biology from the Pontificia Catholic University, Porto Alegre, Brazil, in 2010 and the other is an International Ph.D. in Bioengineering from the Universidad Miguel Hernandez, Elche/Alicante, Spain, obtained in 2020. In 2018, he completed a postdoctoral fellowship in Materials Engineering in the NUCLEMAT of the Pontificia Catholic University, Porto Alegre, Brazil. He is currently the Director of the Postgraduate Program in Implantology of the Bioface/UCAM/PgO (Montevideo, Uruguay), Director of the Cathedra of Biotechnology of the Catholic University of Murcia (Murcia, Spain), an Extraordinary Full Professor of the Catholic University of Murcia (Murcia, Spain) as well as the Director of the private center of research Biotecnos – Technology and Science (Montevideo, Uruguay). Applied biomaterials, cellular and molecular biology, and dental implants are among his research interests. He has published several original papers in renowned journals. In addition, he is also a Collaborating Professor in several Postgraduate programs at different universities all over the world.",institutionString:null,institution:{name:"Universidad Católica San Antonio de Murcia",country:{name:"Spain"}}},{id:"342152",title:"Dr.",name:"Santo",middleName:null,surname:"Grace Umesh",slug:"santo-grace-umesh",fullName:"Santo Grace Umesh",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/342152/images/16311_n.jpg",biography:null,institutionString:null,institution:{name:"SRM Dental College",country:{name:"India"}}},{id:"333647",title:"Dr.",name:"Shreya",middleName:null,surname:"Kishore",slug:"shreya-kishore",fullName:"Shreya Kishore",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/333647/images/14701_n.jpg",biography:"Dr. Shreya Kishore completed her Bachelor in Dental Surgery in Chettinad Dental College and Research Institute, Chennai, and her Master of Dental Surgery (Orthodontics) in Saveetha Dental College, Chennai. She is also Invisalign certified. She’s working as a Senior Lecturer in the Department of Orthodontics, SRM Dental College since November 2019. She is actively involved in teaching orthodontics to the undergraduates and the postgraduates. Her clinical research topics include new orthodontic brackets, fixed appliances and TADs. She’s published 4 articles in well renowned indexed journals and has a published patency of her own. Her private practice is currently limited to orthodontics and works as a consultant in various clinics.",institutionString:null,institution:{name:"SRM Dental College",country:{name:"India"}}},{id:"323731",title:"Prof.",name:"Deepak M.",middleName:"Macchindra",surname:"Vikhe",slug:"deepak-m.-vikhe",fullName:"Deepak M. Vikhe",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/323731/images/13613_n.jpg",biography:"Dr Deepak M.Vikhe .\n\n\t\n\tDr Deepak M.Vikhe , completed his Masters & PhD in Prosthodontics from Rural Dental College, Loni securing third rank in the Pravara Institute of Medical Sciences Deemed University. He was awarded Dr.G.C.DAS Memorial Award for Research on Implants at 39th IPS conference Dubai (U A E).He has two patents under his name. He has received Dr.Saraswati medal award for best research for implant study in 2017.He has received Fully funded scholarship to Spain ,university of Santiago de Compostela. He has completed fellowship in Implantlogy from Noble Biocare. \nHe has attended various conferences and CDE programmes and has national publications to his credit. His field of interest is in Implant supported prosthesis. Presently he is working as a associate professor in the Dept of Prosthodontics, Rural Dental College, Loni and maintains a successful private practice specialising in Implantology at Rahata.\n\nEmail: drdeepak_mvikhe@yahoo.com..................",institutionString:null,institution:{name:"Pravara Institute of Medical Sciences",country:{name:"India"}}},{id:"204110",title:"Dr.",name:"Ahmed A.",middleName:null,surname:"Madfa",slug:"ahmed-a.-madfa",fullName:"Ahmed A. Madfa",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/204110/images/system/204110.jpg",biography:"Dr. Madfa is currently Associate Professor of Endodontics at Thamar University and a visiting lecturer at Sana'a University and University of Sciences and Technology. He has more than 6 years of experience in teaching. His research interests include root canal morphology, functionally graded concept, dental biomaterials, epidemiology and dental education, biomimetic restoration, finite element analysis and endodontic regeneration. Dr. Madfa has numerous international publications, full articles, two patents, a book and a book chapter. Furthermore, he won 14 international scientific awards. Furthermore, he is involved in many academic activities ranging from editorial board member, reviewer for many international journals and postgraduate students' supervisor. Besides, I deliver many courses and training workshops at various scientific events. Dr. Madfa also regularly attends international conferences and holds administrative positions (Deputy Dean of the Faculty for Students’ & Academic Affairs and Deputy Head of Research Unit).",institutionString:"Thamar University",institution:null},{id:"210472",title:"Dr.",name:"Nermin",middleName:"Mohammed Ahmed",surname:"Yussif",slug:"nermin-yussif",fullName:"Nermin Yussif",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/210472/images/system/210472.jpg",biography:"Dr. Nermin Mohammed Ahmed Yussif is working at the Faculty of dentistry, University for October university for modern sciences and arts (MSA). Her areas of expertise include: periodontology, dental laserology, oral implantology, periodontal plastic surgeries, oral mesotherapy, nutrition, dental pharmacology. She is an editor and reviewer in numerous international journals.",institutionString:"MSA University",institution:null},{id:"204606",title:"Dr.",name:"Serdar",middleName:null,surname:"Gözler",slug:"serdar-gozler",fullName:"Serdar Gözler",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/204606/images/system/204606.jpeg",biography:"Dr. Serdar Gözler has completed his undergraduate studies at the Marmara University Faculty of Dentistry in 1978, followed by an assistantship in the Prosthesis Department of Dicle University Faculty of Dentistry. Starting his PhD work on non-resilient overdentures with Assoc. Prof. Hüsnü Yavuzyılmaz, he continued his studies with Prof. Dr. Gürbüz Öztürk of Istanbul University Faculty of Dentistry Department of Prosthodontics, this time on Gnatology. He attended training programs on occlusion, neurology, neurophysiology, EMG, radiology and biostatistics. In 1982, he presented his PhD thesis \\Gerber and Lauritzen Occlusion Analysis Techniques: Diagnosis Values,\\ at Istanbul University School of Dentistry, Department of Prosthodontics. As he was also working with Prof. Senih Çalıkkocaoğlu on The Physiology of Chewing at the same time, Gözler has written a chapter in Çalıkkocaoğlu\\'s book \\Complete Prostheses\\ entitled \\The Place of Neuromuscular Mechanism in Prosthetic Dentistry.\\ The book was published five times since by the Istanbul University Publications. Having presented in various conferences about occlusion analysis until 1998, Dr. Gözler has also decided to use the T-Scan II occlusion analysis method. Having been personally trained by Dr. Robert Kerstein on this method, Dr. Gözler has been lecturing on the T-Scan Occlusion Analysis Method in conferences both in Turkey and abroad. Dr. Gözler has various articles and presentations on Digital Occlusion Analysis methods. He is now Head of the TMD Clinic at Prosthodontic Department of Faculty of Dentistry , Istanbul Aydın University , Turkey.",institutionString:"Istanbul Aydin University",institution:{name:"Istanbul Aydın University",country:{name:"Turkey"}}},{id:"240870",title:"Ph.D.",name:"Alaa Eddin Omar",middleName:null,surname:"Al Ostwani",slug:"alaa-eddin-omar-al-ostwani",fullName:"Alaa Eddin Omar Al Ostwani",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/240870/images/system/240870.jpeg",biography:"Dr. Al Ostwani Alaa Eddin Omar received his Master in dentistry from Damascus University in 2010, and his Ph.D. in Pediatric Dentistry from Damascus University in 2014. Dr. Al Ostwani is an assistant professor and faculty member at IUST University since 2014. \nDuring his academic experience, he has received several awards including the scientific research award from the Union of Arab Universities, the Syrian gold medal and the international gold medal for invention and creativity. Dr. Al Ostwani is a Member of the International Association of Dental Traumatology and the Syrian Society for Research and Preventive Dentistry since 2017. He is also a Member of the Reviewer Board of International Journal of Dental Medicine (IJDM), and the Indian Journal of Conservative and Endodontics since 2016.",institutionString:"International University for Science and Technology.",institution:{name:"Islamic University of Science and Technology",country:{name:"India"}}},{id:"42847",title:"Dr.",name:"Belma",middleName:null,surname:"Işik Aslan",slug:"belma-isik-aslan",fullName:"Belma Işik Aslan",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/42847/images/system/42847.jpg",biography:"Dr. Belma IşIk Aslan was born in 1976 in Ankara-TURKEY. After graduating from TED Ankara College in 1994, she attended to Gazi University, Faculty of Dentistry in Ankara. She completed her PhD in orthodontic education at Gazi University between 1999-2005. Dr. Işık Aslan stayed at the Providence Hospital Craniofacial Institude and Reconstructive Surgery in Michigan, USA for three months as an observer. She worked as a specialist doctor at Gazi University, Dentistry Faculty, Department of Orthodontics between 2005-2014. She was appointed as associate professor in January, 2014 and as professor in 2021. Dr. Işık Aslan still works as an instructor at the same faculty. She has published a total of 35 articles, 10 book chapters, 39 conference proceedings both internationally and nationally. Also she was the academic editor of the international book 'Current Advances in Orthodontics'. She is a member of the Turkish Orthodontic Society and Turkish Cleft Lip and Palate Society. She is married and has 2 children. Her knowledge of English is at an advanced level.",institutionString:"Gazi University Dentistry Faculty Department of Orthodontics",institution:null},{id:"178412",title:"Associate Prof.",name:"Guhan",middleName:null,surname:"Dergin",slug:"guhan-dergin",fullName:"Guhan Dergin",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/178412/images/6954_n.jpg",biography:"Assoc. Prof. Dr. Gühan Dergin was born in 1973 in Izmit. He graduated from Marmara University Faculty of Dentistry in 1999. He completed his specialty of OMFS surgery in Marmara University Faculty of Dentistry and obtained his PhD degree in 2006. In 2005, he was invited as a visiting doctor in the Oral and Maxillofacial Surgery Department of the University of North Carolina, USA, where he went on a scholarship. Dr. Dergin still continues his academic career as an associate professor in Marmara University Faculty of Dentistry. He has many articles in international and national scientific journals and chapters in books.",institutionString:null,institution:{name:"Marmara University",country:{name:"Turkey"}}},{id:"178414",title:"Prof.",name:"Yusuf",middleName:null,surname:"Emes",slug:"yusuf-emes",fullName:"Yusuf Emes",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/178414/images/6953_n.jpg",biography:"Born in Istanbul in 1974, Dr. Emes graduated from Istanbul University Faculty of Dentistry in 1997 and completed his PhD degree in Istanbul University faculty of Dentistry Department of Oral and Maxillofacial Surgery in 2005. He has papers published in international and national scientific journals, including research articles on implantology, oroantral fistulas, odontogenic cysts, and temporomandibular disorders. Dr. Emes is currently working as a full-time academic staff in Istanbul University faculty of Dentistry Department of Oral and Maxillofacial Surgery.",institutionString:null,institution:{name:"Istanbul University",country:{name:"Turkey"}}},{id:"192229",title:"Ph.D.",name:"Ana Luiza",middleName:null,surname:"De Carvalho Felippini",slug:"ana-luiza-de-carvalho-felippini",fullName:"Ana Luiza De Carvalho Felippini",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/192229/images/system/192229.jpg",biography:null,institutionString:"University of São Paulo",institution:{name:"University of Sao Paulo",country:{name:"Brazil"}}},{id:"256851",title:"Prof.",name:"Ayşe",middleName:null,surname:"Gülşen",slug:"ayse-gulsen",fullName:"Ayşe Gülşen",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/256851/images/9696_n.jpg",biography:"Dr. Ayşe Gülşen graduated in 1990 from Faculty of Dentistry, University of Ankara and did a postgraduate program at University of Gazi. \nShe worked as an observer and research assistant in Craniofacial Surgery Departments in New York, Providence Hospital in Michigan and Chang Gung Memorial Hospital in Taiwan. \nShe works as Craniofacial Orthodontist in Department of Aesthetic, Plastic and Reconstructive Surgery, Faculty of Medicine, University of Gazi, Ankara Turkey since 2004.",institutionString:"Univeristy of Gazi",institution:null},{id:"255366",title:"Prof.",name:"Tosun",middleName:null,surname:"Tosun",slug:"tosun-tosun",fullName:"Tosun Tosun",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/255366/images/7347_n.jpg",biography:"Graduated at the Faculty of Dentistry, University of Istanbul, Turkey in 1989;\nVisitor Assistant at the University of Padua, Italy and Branemark Osseointegration Center of Treviso, Italy between 1993-94;\nPhD thesis on oral implantology in University of Istanbul and was awarded the academic title “Dr.med.dent.”, 1997;\nHe was awarded the academic title “Doç.Dr.” (Associated Professor) in 2003;\nProficiency in Botulinum Toxin Applications, Reading-UK in 2009;\nMastership, RWTH Certificate in Laser Therapy in Dentistry, AALZ-Aachen University, Germany 2009-11;\nMaster of Science (MSc) in Laser Dentistry, University of Genoa, Italy 2013-14.\n\nDr.Tosun worked as Research Assistant in the Department of Oral Implantology, Faculty of Dentistry, University of Istanbul between 1990-2002. \nHe worked part-time as Consultant surgeon in Harvard Medical International Hospitals and John Hopkins Medicine, Istanbul between years 2007-09.\u2028He was contract Professor in the Department of Surgical and Diagnostic Sciences (DI.S.C.), Medical School, University of Genova, Italy between years 2011-16. \nSince 2015 he is visiting Professor at Medical School, University of Plovdiv, Bulgaria. \nCurrently he is Associated Prof.Dr. at the Dental School, Oral Surgery Dept., Istanbul Aydin University and since 2003 he works in his own private clinic in Istanbul, Turkey.\u2028\nDr.Tosun is reviewer in journal ‘Laser in Medical Sciences’, reviewer in journal ‘Folia Medica\\', a Fellow of the International Team for Implantology, Clinical Lecturer of DGZI German Association of Oral Implantology, Expert Lecturer of Laser&Health Academy, Country Representative of World Federation for Laser Dentistry, member of European Federation of Periodontology, member of Academy of Laser Dentistry. Dr.Tosun presents papers in international and national congresses and has scientific publications in international and national journals. He speaks english, spanish, italian and french.",institutionString:null,institution:{name:"Istanbul Aydın University",country:{name:"Turkey"}}},{id:"171887",title:"Prof.",name:"Zühre",middleName:null,surname:"Akarslan",slug:"zuhre-akarslan",fullName:"Zühre Akarslan",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/171887/images/system/171887.jpg",biography:"Zühre Akarslan was born in 1977 in Cyprus. She graduated from Gazi University Faculty of Dentistry, Ankara, Turkey in 2000. \r\nLater she received her Ph.D. degree from the Oral Diagnosis and Radiology Department; which was recently renamed as Oral and Dentomaxillofacial Radiology, from the same university. \r\nShe is working as a full-time Associate Professor and is a lecturer and an academic researcher. \r\nHer expertise areas are dental caries, cancer, dental fear and anxiety, gag reflex in dentistry, oral medicine, and dentomaxillofacial radiology.",institutionString:"Gazi University",institution:{name:"Gazi University",country:{name:"Turkey"}}},{id:"256417",title:"Associate Prof.",name:"Sanaz",middleName:null,surname:"Sadry",slug:"sanaz-sadry",fullName:"Sanaz Sadry",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/256417/images/8106_n.jpg",biography:null,institutionString:null,institution:null},{id:"272237",title:"Dr.",name:"Pinar",middleName:"Kiymet",surname:"Karataban",slug:"pinar-karataban",fullName:"Pinar Karataban",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/272237/images/8911_n.png",biography:"Assist.Prof.Dr.Pınar Kıymet Karataban, DDS PhD \n\nDr.Pınar Kıymet Karataban was born in Istanbul in 1975. After her graduation from Marmara University Faculty of Dentistry in 1998 she started her PhD in Paediatric Dentistry focused on children with special needs; mainly children with Cerebral Palsy. She finished her pHD thesis entitled \\'Investigation of occlusion via cast analysis and evaluation of dental caries prevalance, periodontal status and muscle dysfunctions in children with cerebral palsy” in 2008. She got her Assist. Proffessor degree in Istanbul Aydın University Paediatric Dentistry Department in 2015-2018. ın 2019 she started her new career in Bahcesehir University, Istanbul as Head of Department of Pediatric Dentistry. In 2020 she was accepted to BAU International University, Batumi as Professor of Pediatric Dentistry. She’s a lecturer in the same university meanwhile working part-time in private practice in Ege Dental Studio (https://www.egedisklinigi.com/) a multidisciplinary dental clinic in Istanbul. Her main interests are paleodontology, ancient and contemporary dentistry, oral microbiology, cerebral palsy and special care dentistry. She has national and international publications, scientific reports and is a member of IAPO (International Association for Paleodontology), IADH (International Association of Disability and Oral Health) and EAPD (European Association of Pediatric Dentistry).",institutionString:null,institution:null},{id:"202198",title:"Dr.",name:"Buket",middleName:null,surname:"Aybar",slug:"buket-aybar",fullName:"Buket Aybar",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/202198/images/6955_n.jpg",biography:"Buket Aybar, DDS, PhD, was born in 1971. She graduated from Istanbul University, Faculty of Dentistry, in 1992 and completed her PhD degree on Oral and Maxillofacial Surgery in Istanbul University in 1997.\nDr. Aybar is currently a full-time professor in Istanbul University, Faculty of Dentistry Department of Oral and Maxillofacial Surgery. She has teaching responsibilities in graduate and postgraduate programs. Her clinical practice includes mainly dentoalveolar surgery.\nHer topics of interest are biomaterials science and cell culture studies. She has many articles in international and national scientific journals and chapters in books; she also has participated in several scientific projects supported by Istanbul University Research fund.",institutionString:null,institution:null},{id:"260116",title:"Dr.",name:"Mehmet",middleName:null,surname:"Yaltirik",slug:"mehmet-yaltirik",fullName:"Mehmet Yaltirik",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/260116/images/7413_n.jpg",biography:"Birth Date 25.09.1965\r\nBirth Place Adana- Turkey\r\nSex Male\r\nMarrial Status Bachelor\r\nDriving License Acquired\r\nMother Tongue Turkish\r\n\r\nAddress:\r\nWork:University of Istanbul,Faculty of Dentistry, Department of Oral Surgery and Oral Medicine 34093 Capa,Istanbul- TURKIYE",institutionString:null,institution:null},{id:"172009",title:"Dr.",name:"Fatma Deniz",middleName:null,surname:"Uzuner",slug:"fatma-deniz-uzuner",fullName:"Fatma Deniz Uzuner",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/172009/images/7122_n.jpg",biography:"Dr. Deniz Uzuner was born in 1969 in Kocaeli-TURKEY. After graduating from TED Ankara College in 1986, she attended the Hacettepe University, Faculty of Dentistry in Ankara. \nIn 1993 she attended the Gazi University, Faculty of Dentistry, Department of Orthodontics for her PhD education. After finishing the PhD education, she worked as orthodontist in Ankara Dental Hospital under the Turkish Government, Ministry of Health and in a special Orthodontic Clinic till 2011. Between 2011 and 2016, Dr. Deniz Uzuner worked as a specialist in the Department of Orthodontics, Faculty of Dentistry, Gazi University in Ankara/Turkey. In 2016, she was appointed associate professor. Dr. Deniz Uzuner has authored 23 Journal Papers, 3 Book Chapters and has had 39 oral/poster presentations. She is a member of the Turkish Orthodontic Society. Her knowledge of English is at an advanced level.",institutionString:null,institution:null},{id:"332914",title:"Dr.",name:"Muhammad Saad",middleName:null,surname:"Shaikh",slug:"muhammad-saad-shaikh",fullName:"Muhammad Saad Shaikh",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Jinnah Sindh Medical University",country:{name:"Pakistan"}}},{id:"315775",title:"Dr.",name:"Feng",middleName:null,surname:"Luo",slug:"feng-luo",fullName:"Feng Luo",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Sichuan University",country:{name:"China"}}},{id:"423519",title:"Dr.",name:"Sizakele",middleName:null,surname:"Ngwenya",slug:"sizakele-ngwenya",fullName:"Sizakele Ngwenya",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"University of the Witwatersrand",country:{name:"South Africa"}}},{id:"419270",title:"Dr.",name:"Ann",middleName:null,surname:"Chianchitlert",slug:"ann-chianchitlert",fullName:"Ann Chianchitlert",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Walailak University",country:{name:"Thailand"}}},{id:"419271",title:"Dr.",name:"Diane",middleName:null,surname:"Selvido",slug:"diane-selvido",fullName:"Diane Selvido",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Walailak University",country:{name:"Thailand"}}},{id:"419272",title:"Dr.",name:"Irin",middleName:null,surname:"Sirisoontorn",slug:"irin-sirisoontorn",fullName:"Irin Sirisoontorn",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Walailak University",country:{name:"Thailand"}}},{id:"355660",title:"Dr.",name:"Anitha",middleName:null,surname:"Mani",slug:"anitha-mani",fullName:"Anitha Mani",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"SRM Dental College",country:{name:"India"}}},{id:"355612",title:"Dr.",name:"Janani",middleName:null,surname:"Karthikeyan",slug:"janani-karthikeyan",fullName:"Janani Karthikeyan",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"SRM Dental College",country:{name:"India"}}},{id:"334400",title:"Dr.",name:"Suvetha",middleName:null,surname:"Siva",slug:"suvetha-siva",fullName:"Suvetha Siva",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"SRM Dental College",country:{name:"India"}}}]}},subseries:{item:{id:"22",type:"subseries",title:"Applied Intelligence",keywords:"Machine Learning, Intelligence Algorithms, Data Science, Artificial Intelligence, Applications on Applied Intelligence",scope:"This field is the key in the current industrial revolution (Industry 4.0), where the new models and developments are based on the knowledge generation on applied intelligence. The motor of the society is the industry and the research of this topic has to be empowered in order to increase and improve the quality of our lives.",coverUrl:"https://cdn.intechopen.com/series_topics/covers/22.jpg",hasOnlineFirst:!0,hasPublishedBooks:!0,annualVolume:11418,editor:{id:"27170",title:"Prof.",name:"Carlos",middleName:"M.",surname:"Travieso-Gonzalez",slug:"carlos-travieso-gonzalez",fullName:"Carlos Travieso-Gonzalez",profilePictureURL:"https://mts.intechopen.com/storage/users/27170/images/system/27170.jpeg",biography:"Carlos M. Travieso-González received his MSc degree in Telecommunication Engineering at Polytechnic University of Catalonia (UPC), Spain in 1997, and his Ph.D. degree in 2002 at the University of Las Palmas de Gran Canaria (ULPGC-Spain). He is a full professor of signal processing and pattern recognition and is head of the Signals and Communications Department at ULPGC, teaching from 2001 on subjects on signal processing and learning theory. His research lines are biometrics, biomedical signals and images, data mining, classification system, signal and image processing, machine learning, and environmental intelligence. He has researched in 52 international and Spanish research projects, some of them as head researcher. He is co-author of 4 books, co-editor of 27 proceedings books, guest editor for 8 JCR-ISI international journals, and up to 24 book chapters. He has over 450 papers published in international journals and conferences (81 of them indexed on JCR – ISI - Web of Science). He has published seven patents in the Spanish Patent and Trademark Office. He has been a supervisor on 8 Ph.D. theses (11 more are under supervision), and 130 master theses. He is the founder of The IEEE IWOBI conference series and the president of its Steering Committee, as well as the founder of both the InnoEducaTIC and APPIS conference series. He is an evaluator of project proposals for the European Union (H2020), Medical Research Council (MRC, UK), Spanish Government (ANECA, Spain), Research National Agency (ANR, France), DAAD (Germany), Argentinian Government, and the Colombian Institutions. He has been a reviewer in different indexed international journals (<70) and conferences (<250) since 2001. He has been a member of the IASTED Technical Committee on Image Processing from 2007 and a member of the IASTED Technical Committee on Artificial Intelligence and Expert Systems from 2011. \n\nHe has held the general chair position for the following: ACM-APPIS (2020, 2021), IEEE-IWOBI (2019, 2020 and 2020), A PPIS (2018, 2019), IEEE-IWOBI (2014, 2015, 2017, 2018), InnoEducaTIC (2014, 2017), IEEE-INES (2013), NoLISP (2011), JRBP (2012), and IEEE-ICCST (2005)\n\nHe is an associate editor of the Computational Intelligence and Neuroscience Journal (Hindawi – Q2 JCR-ISI). He was vice dean from 2004 to 2010 in the Higher Technical School of Telecommunication Engineers at ULPGC and the vice dean of Graduate and Postgraduate Studies from March 2013 to November 2017. He won the “Catedra Telefonica” Awards in Modality of Knowledge Transfer, 2017, 2018, and 2019 editions, and awards in Modality of COVID Research in 2020.\n\nPublic References:\nResearcher ID http://www.researcherid.com/rid/N-5967-2014\nORCID https://orcid.org/0000-0002-4621-2768 \nScopus Author ID https://www.scopus.com/authid/detail.uri?authorId=6602376272\nScholar Google https://scholar.google.es/citations?user=G1ks9nIAAAAJ&hl=en \nResearchGate https://www.researchgate.net/profile/Carlos_Travieso",institutionString:null,institution:{name:"University of Las Palmas de Gran Canaria",institutionURL:null,country:{name:"Spain"}}},editorTwo:null,editorThree:null,series:{id:"14",title:"Artificial Intelligence",doi:"10.5772/intechopen.79920",issn:"2633-1403"},editorialBoard:[{id:"13633",title:"Prof.",name:"Abdelhamid",middleName:null,surname:"Mellouk",slug:"abdelhamid-mellouk",fullName:"Abdelhamid Mellouk",profilePictureURL:"https://mts.intechopen.com/storage/users/13633/images/1567_n.jpg",institutionString:null,institution:{name:"Paris 12 Val de Marne University",institutionURL:null,country:{name:"France"}}},{id:"109268",title:"Dr.",name:"Ali",middleName:null,surname:"Al-Ataby",slug:"ali-al-ataby",fullName:"Ali Al-Ataby",profilePictureURL:"https://mts.intechopen.com/storage/users/109268/images/7410_n.jpg",institutionString:null,institution:{name:"University of Liverpool",institutionURL:null,country:{name:"United Kingdom"}}},{id:"3807",title:"Dr.",name:"Carmelo",middleName:"Jose Albanez",surname:"Bastos-Filho",slug:"carmelo-bastos-filho",fullName:"Carmelo Bastos-Filho",profilePictureURL:"https://mts.intechopen.com/storage/users/3807/images/624_n.jpg",institutionString:null,institution:{name:"Universidade de Pernambuco",institutionURL:null,country:{name:"Brazil"}}},{id:"38850",title:"Dr.",name:"Efren",middleName:null,surname:"Gorrostieta Hurtado",slug:"efren-gorrostieta-hurtado",fullName:"Efren Gorrostieta Hurtado",profilePictureURL:"https://mts.intechopen.com/storage/users/38850/images/system/38850.jpg",institutionString:null,institution:{name:"Autonomous University of Queretaro",institutionURL:null,country:{name:"Mexico"}}},{id:"239041",title:"Prof.",name:"Yang",middleName:null,surname:"Yi",slug:"yang-yi",fullName:"Yang Yi",profilePictureURL:"https://mts.intechopen.com/storage/users/239041/images/system/239041.jpeg",institutionString:"Virginia Tech",institution:{name:"Virginia Tech",institutionURL:null,country:{name:"United States of America"}}}]},onlineFirstChapters:{paginationCount:1,paginationItems:[{id:"81321",title:"Velocity Planning via Model-Based Reinforcement Learning: Demonstrating Results on PILCO for One-Dimensional Linear Motion with Bounded Acceleration",doi:"10.5772/intechopen.103690",signatures:"Hsuan-Cheng Liao, Han-Jung Chou and Jing-Sin Liu",slug:"velocity-planning-via-model-based-reinforcement-learning-demonstrating-results-on-pilco-for-one-dime",totalDownloads:3,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Applied Intelligence - Annual Volume 2022",coverURL:"https://cdn.intechopen.com/books/images_new/11418.jpg",subseries:{id:"22",title:"Applied Intelligence"}}}]},publishedBooks:{},testimonialsList:[{id:"18",text:"It was great publishing with IntechOpen, the process was straightforward and I had support all along.",author:{id:"71579",name:"Berend",surname:"Olivier",institutionString:"Utrecht University",profilePictureURL:"https://mts.intechopen.com/storage/users/71579/images/system/71579.png",slug:"berend-olivier",institution:{id:"253",name:"Utrecht University",country:{id:null,name:"Netherlands"}}}},{id:"27",text:"The opportunity to work with a prestigious publisher allows for the possibility to collaborate with more research groups interested in animal nutrition, leading to the development of new feeding strategies and food valuation while being more sustainable with the environment, allowing more readers to learn about the subject.",author:{id:"175967",name:"Manuel",surname:"Gonzalez Ronquillo",institutionString:null,profilePictureURL:"https://mts.intechopen.com/storage/users/175967/images/system/175967.png",slug:"manuel-gonzalez-ronquillo",institution:{id:"6221",name:"Universidad Autónoma del Estado de México",country:{id:null,name:"Mexico"}}}},{id:"8",text:"I work with IntechOpen for a number of reasons: their professionalism, their mission in support of Open Access publishing, and the quality of their peer-reviewed publications, but also because they believe in equality.",author:{id:"202192",name:"Catrin",surname:"Rutland",institutionString:null,profilePictureURL:"https://mts.intechopen.com/storage/users/202192/images/system/202192.png",slug:"catrin-rutland",institution:{id:"134",name:"University of Nottingham",country:{id:null,name:"United Kingdom"}}}}]},submityourwork:{pteSeriesList:[{id:"14",title:"Artificial Intelligence",numberOfPublishedBooks:9,numberOfPublishedChapters:89,numberOfOpenTopics:6,numberOfUpcomingTopics:0,issn:"2633-1403",doi:"10.5772/intechopen.79920",isOpenForSubmission:!0},{id:"7",title:"Biomedical Engineering",numberOfPublishedBooks:12,numberOfPublishedChapters:104,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2631-5343",doi:"10.5772/intechopen.71985",isOpenForSubmission:!0}],lsSeriesList:[{id:"11",title:"Biochemistry",numberOfPublishedBooks:32,numberOfPublishedChapters:318,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2632-0983",doi:"10.5772/intechopen.72877",isOpenForSubmission:!0},{id:"25",title:"Environmental Sciences",numberOfPublishedBooks:1,numberOfPublishedChapters:12,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2754-6713",doi:"10.5772/intechopen.100362",isOpenForSubmission:!0},{id:"10",title:"Physiology",numberOfPublishedBooks:11,numberOfPublishedChapters:141,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2631-8261",doi:"10.5772/intechopen.72796",isOpenForSubmission:!0}],hsSeriesList:[{id:"3",title:"Dentistry",numberOfPublishedBooks:8,numberOfPublishedChapters:129,numberOfOpenTopics:2,numberOfUpcomingTopics:0,issn:"2631-6218",doi:"10.5772/intechopen.71199",isOpenForSubmission:!0},{id:"6",title:"Infectious Diseases",numberOfPublishedBooks:13,numberOfPublishedChapters:113,numberOfOpenTopics:3,numberOfUpcomingTopics:1,issn:"2631-6188",doi:"10.5772/intechopen.71852",isOpenForSubmission:!0},{id:"13",title:"Veterinary Medicine and Science",numberOfPublishedBooks:11,numberOfPublishedChapters:105,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2632-0517",doi:"10.5772/intechopen.73681",isOpenForSubmission:!0}],sshSeriesList:[{id:"22",title:"Business, Management and Economics",numberOfPublishedBooks:1,numberOfPublishedChapters:19,numberOfOpenTopics:2,numberOfUpcomingTopics:1,issn:"2753-894X",doi:"10.5772/intechopen.100359",isOpenForSubmission:!0},{id:"23",title:"Education and Human Development",numberOfPublishedBooks:0,numberOfPublishedChapters:5,numberOfOpenTopics:1,numberOfUpcomingTopics:1,issn:null,doi:"10.5772/intechopen.100360",isOpenForSubmission:!0},{id:"24",title:"Sustainable Development",numberOfPublishedBooks:0,numberOfPublishedChapters:15,numberOfOpenTopics:5,numberOfUpcomingTopics:0,issn:null,doi:"10.5772/intechopen.100361",isOpenForSubmission:!0}],subseriesList:[],annualVolumeBook:{},thematicCollection:[],selectedSeries:null,selectedSubseries:null},seriesLanding:{item:null},libraryRecommendation:{success:null,errors:{},institutions:[]},route:{name:"profile.detail",path:"/profiles/287492",hash:"",query:{},params:{id:"287492"},fullPath:"/profiles/287492",meta:{},from:{name:null,path:"/",hash:"",query:{},params:{},fullPath:"/",meta:{}}}},function(){var e;(e=document.currentScript||document.scripts[document.scripts.length-1]).parentNode.removeChild(e)}()