\r\n\toxygen-free atmosphere. Biochar has been used for many years as a soil amendment and in general soil applications. Nonetheless, biochar is far more than a mere soil amendment. In this review, we report all the applications of biochar including environmental remediation, energy storage, composites, and catalyst production. In this book, we intend to collect contributions from worldwide experts in the field of biochar production and utilization providing a general overview of the recent uses of biochar in material science, thus presenting this cheap and waste-derived material as a high value-added carbonaceous source. Furthermore, we are aiming to give readers a handy and effective tool to easily understand how this field is interesting and diverse. It is a goal that this book could be easily used by any reader with a strong scientific background ranging from scientific company advisors to academic members. Nonetheless, students enrolled in scientific undergraduate and graduate programs could be consulted to this text for any further and deeper investigation. In the end, we intend to propose a very high scientific content book that could represent the reference text for any consideration and future study about biochar for the next years.
",isbn:"978-1-80356-252-0",printIsbn:"978-1-80356-251-3",pdfIsbn:"978-1-80356-253-7",doi:null,price:0,priceEur:0,priceUsd:0,slug:null,numberOfPages:0,isOpenForSubmission:!1,isSalesforceBook:!1,isNomenclature:!1,hash:"af29d12de2a10f46f574776213120e9e",bookSignature:"Dr. Mattia Bartoli, Dr. Mauro Giorcelli and Prof. Alberto Tagliaferro",publishedDate:null,coverURL:"https://cdn.intechopen.com/books/images_new/11537.jpg",keywords:"Energy Storage, Battery, Environmental Remediation, Catalysis, Reactors, Fast Pyrolysis, Slow Pyrolysis, Microwave Pyrolysis, Porosity, Raman, Monolith, Hard Carbon",numberOfDownloads:79,numberOfWosCitations:0,numberOfCrossrefCitations:0,numberOfDimensionsCitations:0,numberOfTotalCitations:0,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"February 10th 2022",dateEndSecondStepPublish:"April 13th 2022",dateEndThirdStepPublish:"June 12th 2022",dateEndFourthStepPublish:"August 31st 2022",dateEndFifthStepPublish:"October 30th 2022",dateConfirmationOfParticipation:null,remainingDaysToSecondStep:"3 months",secondStepPassed:!0,areRegistrationsClosed:!0,currentStepOfPublishingProcess:4,editedByType:null,kuFlag:!1,biosketch:"A cutting-edge researcher in waste-to-value technologies for application in drop-in fuels, green chemicals, and material science. 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\n\t\t\t
1. Introduction
\n\t\t\t
Systemic insecticides were first developed in the 1950s, with the introduction of soluble organophosphorus (OP) compounds such as dimethoate, demeton-S-methyl, mevinphos and phorate. They were valuable in controlling sucking pests and burrowing larvae in many crops, their main advantage being their translocation to all tissues of the treated plant. Systemic carbamates followed in the 1960s with aldicarb and carbofuran. Since then, both insecticidal classes comprise a large number of broad-spectrum insecticides used in agriculture all over the world. Nowadays, OPs are the most common pesticides used in tropical, developing countries such as the Philippines and Vietnam, where 22 and 17% of the respective agrochemicals are ‘extremely hazardous’ [126], i.e. classified as WHO class I. Systemic insect growth regulators were developed during the 1980-90s, and comprise only a handful of compounds, which are more selective than their predecessors. Since 1990 onwards, cartap, fipronil and neonicotinoids are replacing the old hazardous chemicals in most developed and developing countries alike [137].
\n\t\t\t
Through seed coatings and granular applications, systemic insecticides pose minimal risk of pesticide drift or worker exposure in agricultural, nurseries and urban settings. Neonicotinoids and fipronil are also preferred because they appear to be less toxic to fish and terrestrial vertebrates. Initially proposed as environmentally friendly agrochemicals [129], their use in Integrated Pest Management (IPM) programs has been questioned by recent research that shows their negative impact on predatory and parasitic agents [221, 258, 299]. New formulations have been developed to optimize the bioavailability of neonicotinoids, as well as combined formulations with pyrethroids and other insecticides with the aim of broadening the insecticidal spectrum and avoid resistance by pests [83]. Indeed, as with any other chemical used in pest control, resistance to imidacloprid by whitefly (Bemisia tabaci), cotton aphids (Aphis gossypii) and other pests is rendering ineffective this and other neonicotinoids such as acetamiprid, thiacloprid and nitenpyram [247, 269].
\n\t\t\t
This chapter examines the negative impacts that systemic insecticides have on organisms, populations and ecosystems. The efficacy of these products in controlling the target pests is assumed and not dealt with here – only the effects on non-target organisms and communities are considered.
\n\t\t
\n\t\t
\n\t\t\t
2. Exposure to systemic insecticides
\n\t\t\t
Unlike typical contact insecticides, that are usually taken up through the arthropod’s cuticle or skin of animals, systemic insecticides get into the organisms mainly through feeding on the treated plants or contaminated soil. Thus, monocrotophos and imidacloprid are more lethal to honey bees (Apis mellifera) through feeding than contact exposure [143]. Residual or contact exposure affects also some pests and non-target species alike.
\n\t\t\t
Systemic insecticides are applied directly to the crop soil and seedlings in glasshouses using flowable solutions or granules, and often as seed-dressings, with foliar applications and drenching being less common. Being quite water soluble (Table 1), these insecticides are readily taken up by the plant roots or incorporated into the tissues of the growing plants as they develop, so the pests that come to eat them ingest a lethal dose and die. Sucking insects in particular are fatally exposed to systemic insecticides, as sap carries the most concentrated fraction of the poisonous chemical for a few weeks [124], whereas leaf-eating species such as citrus thrips and red mites may not be affected [30]. Systemic insecticides contaminate all plant tissues, from the roots to leaves and flowers, where active residues can be found up to 45-90 days [175, 187], lasting as long as in soil. Thus, pollen and nectar of the flowers get contaminated [33], and residues of imidacloprid and aldicarb have been found at levels above 1 mg/kg in the United States [200]. Guttation drops, in particular, can be contaminated with residues as high as 100-345 mg/L of neonicotinoids during 10-15 days following application [272]. Because these insecticides are incorporated in the flesh of fruits, the highly poisonous aldicarb is prohibited in edible crops such as watermelons, as it has caused human poisoning [106].
\n\t\t\t
As with all poisonous chemicals spread in the environment, not only the target insect pests get affected: any other organism that feeds on the treated plants receives a dose as well, and may die or suffer sublethal effects. For example, uptake of aldicarb by plants and worms results in contamination of the vertebrate fauna up to 90 days after application [41], and honey bees may collect pollen contaminated with neonicotinoids to feed their larvae, which are thus poisoned and die [125]. Newly emerged worker bees are most susceptible to insecticides, followed by foraging workers, while nursery workers are the least susceptible within 72 h of treatment [80]. Insects and mites can negatively be affected by systemic insecticides whenever they feed on:
\n\t\t\t
pollen, nectar, plant tissue, sap or guttation drops contaminated with the active ingredient (primary poisoning);
prey or hosts that have consumed leaves contaminated with the active ingredient (secondary poisoning).
\n\t\t\t
Parasitoids may be indirectly affected because foliar, drench or granular applications may decrease host population to levels that are not enough to sustain them. Furthermore, host quality may be unacceptable for egg laying by parasitoid females [54]. Small insectivorous animals (e.g. amphibians, reptiles, birds, shrews and bats) can also suffer from primary poisoning if the residual insecticide or its metabolites in the prey are still active. It should be noticed that some metabolites of imidacloprid, thiamethoxam, fipronil and 50% of carbamates are as toxic as the parent compounds [29]. Thus, two species of predatory miridbugs were negatively affected by residues and metabolites of fipronil applied to rice crops [159]. However, since systemic insecticides do not bioaccumulate in organisms, there is little risk of secondary poisoning through the food chain.
\n\t\t\n\t\t\t
Apart from feeding, direct contact exposure may also occur when the systemic insecticides are sprayed on foliage. In these cases, using a silicone adjuvant (Sylgard 309) reduces the contact exposure of honey bees to carbofuran, methomyl and imidacloprid, but increases it for fipronil [184]. In general the susceptibility of bees to a range of insecticides is: wild bees > honey bee > bumble bee [185]. In reality a combination of both contact and feeding exposure occurs, which is more deadly than either route of exposure alone [152, 218].
\n\t\t\t\n\t\t\t
In soil, residues of acephate and methomyl account for most of the cholinesterase inhibition activity found in mixtures of insecticides [233]. Fortunately, repeated applications of these insecticides induces microbial adaptation, which degrade the active compounds faster over time [250]. Degradation of carbamates and OPs in tropical soils or vegetation is also faster than on temperate regions, due mainly to microbial activity [46]. Some neonicotinoids are degraded by soil microbes [172], and the yeast Rhodotorula mucilaginosa can degrade acetamiprid but none of the other neonicotinoids [63], which are quite persistent in this media (Table 2).
\n\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\n\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\tChemical
\n\t\t\t\t\t\t
Group
\n\t\t\t\t\t\t
Vapour Pressure(mPa, 25oC)
\n\t\t\t\t\t\t
Solubilityin water (mg/L)
\n\t\t\t\t\t\t
Log Kow#
\n\t\t\t\t\t\t
GUS index*
\n\t\t\t\t\t\t
Leaching potential
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
aldicarb
\n\t\t\t\t\t\t
C
\n\t\t\t\t\t\t
3.87
\n\t\t\t\t\t\t
4930
\n\t\t\t\t\t\t
1.15
\n\t\t\t\t\t\t
2.52
\n\t\t\t\t\t\t
moderate
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
bendiocarb
\n\t\t\t\t\t\t
C
\n\t\t\t\t\t\t
4.6
\n\t\t\t\t\t\t
280
\n\t\t\t\t\t\t
1.72
\n\t\t\t\t\t\t
0.77
\n\t\t\t\t\t\t
low
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
butocarboxim
\n\t\t\t\t\t\t
C
\n\t\t\t\t\t\t
10.6
\n\t\t\t\t\t\t
35000
\n\t\t\t\t\t\t
1.1
\n\t\t\t\t\t\t
1.32
\n\t\t\t\t\t\t
low
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
butoxycarboxim
\n\t\t\t\t\t\t
C
\n\t\t\t\t\t\t
0.266
\n\t\t\t\t\t\t
209000
\n\t\t\t\t\t\t
-0.81
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t4.87\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
high
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
carbofuran
\n\t\t\t\t\t\t
C
\n\t\t\t\t\t\t
0.08
\n\t\t\t\t\t\t
322
\n\t\t\t\t\t\t
1.8
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t3.02\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
high
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
ethiofencarb
\n\t\t\t\t\t\t
C
\n\t\t\t\t\t\t
0.5
\n\t\t\t\t\t\t
1900
\n\t\t\t\t\t\t
2.04
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t3.58\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
high
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
methomyl
\n\t\t\t\t\t\t
C
\n\t\t\t\t\t\t
0.72
\n\t\t\t\t\t\t
55000
\n\t\t\t\t\t\t
0.09
\n\t\t\t\t\t\t
2.20
\n\t\t\t\t\t\t
marginal
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
oxamyl
\n\t\t\t\t\t\t
C
\n\t\t\t\t\t\t
0.051
\n\t\t\t\t\t\t
148100
\n\t\t\t\t\t\t
-0.44
\n\t\t\t\t\t\t
2.36
\n\t\t\t\t\t\t
moderate
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
pirimicarb
\n\t\t\t\t\t\t
C
\n\t\t\t\t\t\t
0.43
\n\t\t\t\t\t\t
3100
\n\t\t\t\t\t\t
1.7
\n\t\t\t\t\t\t
2.73
\n\t\t\t\t\t\t
moderate
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
thiodicarb
\n\t\t\t\t\t\t
C
\n\t\t\t\t\t\t
5.7
\n\t\t\t\t\t\t
22.2
\n\t\t\t\t\t\t
1.62
\n\t\t\t\t\t\t
-0.24
\n\t\t\t\t\t\t
low
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
thiofanox
\n\t\t\t\t\t\t
C
\n\t\t\t\t\t\t
22.6
\n\t\t\t\t\t\t
5200
\n\t\t\t\t\t\t
2.16
\n\t\t\t\t\t\t
1.67
\n\t\t\t\t\t\t
low
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
triazamate
\n\t\t\t\t\t\t
C
\n\t\t\t\t\t\t
0.13
\n\t\t\t\t\t\t
433
\n\t\t\t\t\t\t
2.59
\n\t\t\t\t\t\t
-0.9
\n\t\t\t\t\t\t
low
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
cartap
\n\t\t\t\t\t\t
D
\n\t\t\t\t\t\t
1.0 x 10-10\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
200000
\n\t\t\t\t\t\t
-0.95
\n\t\t\t\t\t\t
-
\n\t\t\t\t\t\t
high
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
halofenozide
\n\t\t\t\t\t\t
IGR
\n\t\t\t\t\t\t
<0.013
\n\t\t\t\t\t\t
12.3
\n\t\t\t\t\t\t
3.34
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t3.75\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
high
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
hexaflumuron
\n\t\t\t\t\t\t
IGR
\n\t\t\t\t\t\t
0.059
\n\t\t\t\t\t\t
0.027
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t5.68 \n\t\t\t\t\t\t
\n\t\t\t\t\t\t
-0.03
\n\t\t\t\t\t\t
unlikely to leach
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
novaluron
\n\t\t\t\t\t\t
IGR
\n\t\t\t\t\t\t
0.016
\n\t\t\t\t\t\t
0.003
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t4.3\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
0.03
\n\t\t\t\t\t\t
low
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
teflubenzuron
\n\t\t\t\t\t\t
IGR
\n\t\t\t\t\t\t
0.000013
\n\t\t\t\t\t\t
0.01
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t4.3\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
-0.82
\n\t\t\t\t\t\t
low
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
acetamiprid
\n\t\t\t\t\t\t
N
\n\t\t\t\t\t\t
0.000173
\n\t\t\t\t\t\t
2950
\n\t\t\t\t\t\t
0.8
\n\t\t\t\t\t\t
0.94
\n\t\t\t\t\t\t
low
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
clothianidin
\n\t\t\t\t\t\t
N
\n\t\t\t\t\t\t
2.8 x 10-8\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
340
\n\t\t\t\t\t\t
0.905
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t4.91\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
high
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
dinotefuran
\n\t\t\t\t\t\t
N
\n\t\t\t\t\t\t
0.0017
\n\t\t\t\t\t\t
39830
\n\t\t\t\t\t\t
-0.549
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t4.95\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
high
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
imidacloprid
\n\t\t\t\t\t\t
N
\n\t\t\t\t\t\t
0.0000004
\n\t\t\t\t\t\t
610
\n\t\t\t\t\t\t
0.57
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t3.76\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
high
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
nitenpyram
\n\t\t\t\t\t\t
N
\n\t\t\t\t\t\t
0.0011
\n\t\t\t\t\t\t
590000
\n\t\t\t\t\t\t
-0.66
\n\t\t\t\t\t\t
2.01
\n\t\t\t\t\t\t
moderate
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
thiacloprid
\n\t\t\t\t\t\t
N
\n\t\t\t\t\t\t
0.0000003
\n\t\t\t\t\t\t
184
\n\t\t\t\t\t\t
1.26
\n\t\t\t\t\t\t
1.44
\n\t\t\t\t\t\t
unlikely to leach
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
thiamethoxam
\n\t\t\t\t\t\t
N
\n\t\t\t\t\t\t
0.0000066
\n\t\t\t\t\t\t
4100
\n\t\t\t\t\t\t
-0.13
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t3.82\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
high
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
acephate
\n\t\t\t\t\t\t
OP
\n\t\t\t\t\t\t
0.226
\n\t\t\t\t\t\t
790000
\n\t\t\t\t\t\t
-0.85
\n\t\t\t\t\t\t
1.14
\n\t\t\t\t\t\t
low
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
demeton-S-methyl
\n\t\t\t\t\t\t
OP
\n\t\t\t\t\t\t
40
\n\t\t\t\t\t\t
22000
\n\t\t\t\t\t\t
1.32
\n\t\t\t\t\t\t
0.88
\n\t\t\t\t\t\t
low
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
dicrotophos
\n\t\t\t\t\t\t
OP
\n\t\t\t\t\t\t
9.3
\n\t\t\t\t\t\t
1000000
\n\t\t\t\t\t\t
-0.5
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t3.08\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
high
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
dimethoate
\n\t\t\t\t\t\t
OP
\n\t\t\t\t\t\t
0.25
\n\t\t\t\t\t\t
39800
\n\t\t\t\t\t\t
0.704
\n\t\t\t\t\t\t
1.06
\n\t\t\t\t\t\t
low
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
disulfoton
\n\t\t\t\t\t\t
OP
\n\t\t\t\t\t\t
7.2
\n\t\t\t\t\t\t
25
\n\t\t\t\t\t\t
3.95
\n\t\t\t\t\t\t
1.29
\n\t\t\t\t\t\t
low
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
fenamiphos
\n\t\t\t\t\t\t
OP
\n\t\t\t\t\t\t
0.12
\n\t\t\t\t\t\t
345
\n\t\t\t\t\t\t
3.3
\n\t\t\t\t\t\t
-0.11
\n\t\t\t\t\t\t
low
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
fosthiazate
\n\t\t\t\t\t\t
OP
\n\t\t\t\t\t\t
0.56
\n\t\t\t\t\t\t
9000
\n\t\t\t\t\t\t
1.68
\n\t\t\t\t\t\t
2.48
\n\t\t\t\t\t\t
moderate
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
heptenophos
\n\t\t\t\t\t\t
OP
\n\t\t\t\t\t\t
65
\n\t\t\t\t\t\t
2200
\n\t\t\t\t\t\t
2.32
\n\t\t\t\t\t\t
0.26
\n\t\t\t\t\t\t
low
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
methamidophos
\n\t\t\t\t\t\t
OP
\n\t\t\t\t\t\t
2.3
\n\t\t\t\t\t\t
200000
\n\t\t\t\t\t\t
-0.79
\n\t\t\t\t\t\t
2.18
\n\t\t\t\t\t\t
moderate
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
mevinphos
\n\t\t\t\t\t\t
OP
\n\t\t\t\t\t\t
17
\n\t\t\t\t\t\t
600000
\n\t\t\t\t\t\t
0.127
\n\t\t\t\t\t\t
0.19
\n\t\t\t\t\t\t
low
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
monocrotophos
\n\t\t\t\t\t\t
OP
\n\t\t\t\t\t\t
0.29
\n\t\t\t\t\t\t
818000
\n\t\t\t\t\t\t
-0.22
\n\t\t\t\t\t\t
2.3
\n\t\t\t\t\t\t
moderate
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
omethoate
\n\t\t\t\t\t\t
OP
\n\t\t\t\t\t\t
3.3
\n\t\t\t\t\t\t
10000
\n\t\t\t\t\t\t
-0.74
\n\t\t\t\t\t\t
2.73
\n\t\t\t\t\t\t
moderate
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
oxydemeton-methyl
\n\t\t\t\t\t\t
OP
\n\t\t\t\t\t\t
2.0
\n\t\t\t\t\t\t
1200000
\n\t\t\t\t\t\t
-0.74
\n\t\t\t\t\t\t
0.0
\n\t\t\t\t\t\t
low
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
phorate
\n\t\t\t\t\t\t
OP
\n\t\t\t\t\t\t
112
\n\t\t\t\t\t\t
50
\n\t\t\t\t\t\t
3.86
\n\t\t\t\t\t\t
1.4
\n\t\t\t\t\t\t
low
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
phosphamidon
\n\t\t\t\t\t\t
OP
\n\t\t\t\t\t\t
2.93
\n\t\t\t\t\t\t
1000000
\n\t\t\t\t\t\t
0.79
\n\t\t\t\t\t\t
2.39
\n\t\t\t\t\t\t
moderate
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
thiometon
\n\t\t\t\t\t\t
OP
\n\t\t\t\t\t\t
39.9
\n\t\t\t\t\t\t
200
\n\t\t\t\t\t\t
3.15
\n\t\t\t\t\t\t
0.37
\n\t\t\t\t\t\t
low
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
vamidothion
\n\t\t\t\t\t\t
OP
\n\t\t\t\t\t\t
1.0 x 10-10\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
4000000
\n\t\t\t\t\t\t
-4.21
\n\t\t\t\t\t\t
0.55
\n\t\t\t\t\t\t
low
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
fipronil
\n\t\t\t\t\t\t
PP
\n\t\t\t\t\t\t
0.002
\n\t\t\t\t\t\t
3.78
\n\t\t\t\t\t\t
3.75
\n\t\t\t\t\t\t
2.45
\n\t\t\t\t\t\t
moderate
\n\t\t\t\t\t
\n\t\t\t\t
Table 1.
Physicochemical properties of systemic insecticides.
C = carbamates; D = dithiol; IGR = Insect growth regulator; N = neonicotinoid; OP = organophosphate; PP = phenylpyrazole
# Partition coefficients between n-octanol and water (Kow) indicate bioaccumulation potential when Log Kow > 4.
*The Groundwater Ubiquity Score (GUS) is calculated using soil half-life (DT50) and organic-carbon sorption constant (Koc) as follows: GUS = log(DT50) x (4-log Koc). A compound is likely to leach if GUS > 2.8 and unlikely to leach when GUS < 1.8; other values in between indicate that leaching potential is marginal.
Degradation of systemic insecticides expressed as half-lives in days. Compounds with half-lives longer than 100 days are considered persistent (Sources: Footprint database & [284].
\n\t\t\t\t\t\ta for pH 9
C = carbamates; D = dithiol; IGR = Insect growth regulator; N = neonicotinoid; OP = organophosphate; PP = phenylpyrazole
\n\t\t\t
Aquatic organisms take up easily whatever residues reach the waterbodies, through runoff from treated fields or contaminated groundwater. Some 20% systemic insecticides are prone to leaching, and 45% are mobile in wet soils (Table 1). For example, acephate leaches more easily than methamidophos [305], and so acephate should be restricted or avoided in tropical areas and rice crops [46]. Residues of aldicarb and methomyl in groundwater can have sublethal effects in mammals [215]. Even if residue levels of systemic insecticides in rivers and lakes are usually at ppb levels (μg/L), persistent compounds such as fipronil, neonicotinoids and growth regulators can have chronic effects due to their constant presence throughout several months in the agricultural season [123]. For example, about 1-2% of imidacloprid in treated soil moves into runoff after rainfall events, with the highest concentrations recorded at 0.49 mg/L [12]. Systemic carbamates and OPs do not last long in water because they breakdown through photolysis or hydrolysis in a few days, or are taken up and degraded by aquatic plants [100]. In any case, their presence and frequency of detection in water depends on local usage patterns [39, 171]. The acute toxicity of most systemic compounds is enhanced in aquatic insects and shrimp under saline stress [22, 253].
\n\t\t\t\n\t\t\t
A characteristic feature of most systemic insecticides –except carbamates– is their increased toxicity with exposure time, which results from a constant or chronic uptake through either feeding or aquatic exposure (Figure 1). Effects are more pronounced some time after the initial application [16], and could last up to eight months [286]. Also, as a result of chronic intoxication, there may not be limiting toxic concentrations (e.g. NOEC or NOEL) in compounds that have irreversible mechanism of toxicity, since any concentration will produce an effect as long as there is sufficient exposure during the life of the organism [274]. This is precisely their main advantage for pest control: any concentration of imidacloprid in the range 0.2-1.6 ml/L can reduce the population of mango hoppers (Idioscopus spp.) to zero within three weeks [291]. However, it is also the greatest danger for all non-target species affected, e.g. predators, pollinators and parasitoids. By contrast, contact insecticides act usually in single exposures (e.g. spray droplets, pulse contamination after spraying, etc.) and have the highest effects immediately after application.
\n\t\t\t
Figure 1.
Increasing toxicity of several systemic insecticides with time of exposure. LD50 for acephate to Episyrphus bateatus and for methomyl to Bombus terrestris [75]; LC50 for imidacloprid to Cypridopsis vidua [234] and thiacloprid to Sympetrum striolatum [28].
\n\t\t
\n\t\t
\n\t\t\t
3. Modes of action of systemic insecticides
\n\t\t\t
Before describing their impacts on organisms and ecosystems, a description of the mechanisms of toxicity of systemic insecticides is briefly outlined.
\n\t\t\t
\n\t\t\t\t
3.1. Acetylcholinesterase inhibitors
\n\t\t\t\t
Carbamates and organophosphorus compounds are inhibitors of the acetylcholinesterase enzyme (AChE), thus blocking the transmission of the nervous impulse through the neuronal synapses. The binding of carbamates to the enzyme is slowly reversible and temporary, i.e. < 24 h [197], whereas that of alkyl OPs is irreversible. The binding of methyl-OPs does not last as long as that of alkyl-OPs, and this feature is compound specific [182]. Given their mode of action, all these compounds are broad-spectrum insecticides, extremely toxic to most animal taxa, from worms to mammalian vertebrates. Avian species are often more susceptible to these compounds due to relatively low levels of detoxifying enzymes in birds [207, 297]. Thus, recovery of ducklings exposed to a range of carbamate and OP insecticides occurred within eight days after being depressed 25-58% following dosing [91].
\n\t\t\t
\n\t\t\t
\n\t\t\t\t
3.2. Insecticides acting on nicotinic acetylcholine receptors (nAChR)
\n\t\t\t\t
Neonicotinoids are derived from nicotine, which is found in the nightshade family of plants (Solanaceae), and particularly in tobacco (Nicotiana tabacum). They all are agonists of the nicotinic acetylcholinesterase receptor (nAChR), which mediate fast cholinergic synaptic transmission and play roles in many sensory and cognitive processes in invertebrates. Binding of neonicotinoids to these receptors is irreversible in arthropods [40, 307]. Given that nAChRs are embedded in the membrane at the neuronal synapses, their regeneration seems unlikely because neurons do not grow. The lower affinity of neonicotinoids for mammalian nAChRs has been attributed to the different ionic structure of the vertebrate subtypes [283]. The high toxicity of neonicotinoids to insects and worms is comparable to that of pyrethroids, but aquatic crustaceans, particularly waterfleas, are more tolerant [119, 136].
\n\t\t\t\t
Cartap is a dithiol pro-insecticide that converts to nereistoxin, a natural toxin found in marine Nereis molluscs. Both cartap and nereistoxin are antagonists of the nAChR in insects and other arthropods [164], blocking irreversibly the neuronal functions of these receptors. Unlike neonicotinoids, cartap appears to be very toxic to fish and amphibians [235].
\n\t\t\t
\n\t\t\t
\n\t\t\t\t
3.3. GABA-R antagonists (fipronil)
\n\t\t\t\t
Fipronil is a phenylpyrazole antagonist of the γ-aminobutyric acid (GABA)-gated chloride channel, binding irreversibly to this receptor and impeding the nervous transmission [56]. Its mode of action, therefore, appears to be identical to that of cyclodiene organochlorins (e.g. endosulfan), but fipronil is mostly systemic whereas all cyclodienes are insecticides with contact activity. Interestingly, while aquatic organisms (e.g. cladocerans, fish) are quite tolerant of fipronil, vertebrates are more susceptible to this compound than to the old organochlorins [235].
\n\t\t\t
\n\t\t\t
\n\t\t\t\t
3.4. Insect growth regulators (IGR)
\n\t\t\t\t
Hexaflumuron, novaluron and teflubenzuron are the only systemic benzoylureas in the market. They are chitin inhibitors, blocking the biosynthesis of this essential component of the arthropod’s exoskeleton. As a consequence, insects and other arthropods cannot moult and die during their development. Since their mode of action is restricted to arthropods, benzoylureas are not very toxic to any other animal taxa, e.g. molluscs, vertebrates, etc. [235].
\n\t\t\t\t
Halofenozide is the only systemic compound among the hydrazines, a group of chemicals that mimic the steroidal hormone ecdysone, which promotes moulting in arthropods [71]. The premature moulting in larvae of some insect taxa, particularly in Lepidoptera, prevents them from reaching the adult stage. Toxicity of halofenozide is selective to insects only.
\n\t\t\t
\n\t\t
\n\t\t
\n\t\t\t
4. Effects on organisms and ecosystems
\n\t\t\t
\n\t\t\t\t
4.1. Direct effects on organisms
\n\t\t\t\t
Mortality of non-target organisms exposed to insecticides is mostly due to acute toxicity, particularly in the case of carbamates. However, with systemic compounds there are many observations of long-term suppression of populations that suggest a chronic lethal impact over time. The latter impacts are likely due to persistence of residual activity in the soil, foliage or water in the case of reversible toxicants (i.e. carbamates), or to irreversible and persistent binding in other cases. (note: all application rates and concentrations here refer to the active ingredient).
\n\t\t\t\t
\n\t\t\t\t\t
4.1.1. Acetylcholinesterase inhibitors
\n\t\t\t\t\t
These compounds can have serious impacts on soil organisms of various taxa. Aldicarb and phorate applied to a cotton crop soil at 0.5 and 1 kg/ha, respectively, eliminated or reduced significantly non-target mesofauna, including mites and springtails. Populations of the latter taxa were reduced for more than 60 days (phorate) and 114 days (aldicarb) [17, 225], with the highest effects peaking after 18 days [16]. Granular applications of phorate (250 mg/kg dry soil) killed almost all earthworms, Collembola, Acarina, free-living saprophytic and parasitic nematodes and Protozoa, with populations of Collembola recovering only when residues went below 2 mg/kg [300]. After a single aldicarb application to soil at 2.5 g/m2, Gamasina predatory mites went to extinction within a year [148]. Bendiocarb impacts on predaceous arthropods and oribatid mites were less severe and temporary compared to the impacts of non-systemic OPs, but increased trap catches of ants two weeks after application [55], possibly as a result of a longer-term effect. Many soil arthropods, in particular mites and springtails, were the most affected by dimethoate –and its metabolite omethoate– residues in soil after sprays of 1-2 ml/L in the farms of the Zendan valley, Yemen [4]. Similar observations were made when dimethoate was sprayed on vegetation of arable fields [85] or in soil microcosms [180]; the springtail populations recovered but attained lower densities a year later, while their dominance structure had changed. However, dimethoate or phosphamidon applied in mustard fields produced only a temporary decline, compared to the long-lasting effect of monocrotophos [141]. Collembola populations do not seem to be affected by pirimicarb applications on cereal crops [95].
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Earthworm populations were affected initially after application of phorate and carbofuran to turfgrass, but not thiofanox, and their numbers recovered subsequently [53]. Reduction of earthworm populations by bendiocarb was the highest (99% in one week) among 17 insecticides applied at label rates on turfgrass, with significant effects lasting up to 20 weeks [216]. Juveniles and species living in the surface layers or coming to the soil surface to feed (e.g., Lumbricus terrestris) are most affected, since a high degree of exposure is usually found in the first 2.5 cm of soil [288]. However, systemic carbamates can be selective to plant-parasitic nematodes without affecting fungal or microbial communities [296]. Thus, cholinesterase inhibitors do not have significant impacts on bacteria, fungi and protozoa in soil [133], and consequently do not alter the soil biochemical processes [79]. Nevertheless, a combined dimethoate-carbofuran application reduced active hyphal lengths and the number of active bacteria in a treated forest soil [58].
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Populations of beneficial predators can be decimated initially as much as the target pests, but they usually recover quickly. For example, thiodicarb or its degradation product, methomyl, applied at 0.5 kg/ha on soybean crops, significantly reduced populations of the predatory bugs Tropiconabis capsiformis and Nabis roseipennis within two days after treatment only [25]. Demeton-S-methyl reduced populations of predatory insects on strawberry patches, whereas pirimicarb and heptenophos had no significant effect on spiders, staphylinids and anthocorids, or on hymenopteran parasitoids [76]. While populations of web spiders and carabid beetles are severely reduced by dimethoate applied to cabbage fields and cereal crops [144], pirimicarb does not seem to have much impact on these taxa [97, 195], affecting mainly aphids [131]. Pirimicarb on wheat crops does not impact on ladybirds, but larvae of Episyrphus balteatus are affected [135]. By contrast, longer impacts have been observed with acephate applied at 0.5 kg/ha on rice paddies, which reduced populations of predatory bugs (Cyrtorrhinus lividipennis and Paederus fuscipes) for at least 10 days [155]. Similar rates of acephate on rice and soybean crops reduced spiders populations for three weeks, but they recovered afterwards [181]. In addition, acephate is deadly to three species of whitefly parasitoid species [267].
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Direct mortality of bumble bees (Bombus terrestris) in short exposures to dimethoate is much higher than for heptenophos or ethiofencarb [132]. However, what matters most is the chronic toxicity to the entire bee colony not just the workers. For example, methamidophos contaminated syrup (2 mg/L) produced significant losses of eggs and larvae of honey bees without any appreciable loss of workers after one week of exposure; the colonies would recover completely within 13 weeks if the insecticide was applied only once [301], indicating a long-term impact on the colony. Similarly, the mortality of non-target adult chrysomelid beetles (Gastrophysa polygoni) after foliar treatment with dimethoate on the host plants was low (1.9-7.6%), but because this insecticide was most toxic to the egg stage, the overall beetle population decreased over time due to hatching failure [146].
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Primary poisoning of birds and mammals by ingestion of OP and carbamate granules or coated seeds is still a problem despite the many attempts to reduce these impacts [189, 190]. For example, mortality of birds that ingested granules of carbofuran in a corn field was extensive, affecting waterfowl, small songbirds and mice within 24 hours. Residues up to 17 mg/kg body weight (b.w.) were found in the dead animals [19]. The granular formulation of this carbamate was banned in the mid-1990s by the US EPA after numerous cases of direct poisoning by animals; however, the liquid formulation applied to alfalfa and corn is just as deadly to bees, because this systemic insecticide is present in the pollen of those plants [208]. Phosphamidon sprayed at 1 kg/ha to larch forests in Switzerland caused many bird deaths [243]; large bird mortality was also observed in Canadian spruce forests sprayed with phosphamidon (0.55 kg/ha), particularly among insectivorous warblers. There was good evidence that birds picked up the insecticide from sprayed foliage within a few hours of application [94]. Carbofuran and phosphamidon were the most common pesticides implicated in deaths of wild birds in Korea between 1998-2002 [157], and ducklings died in large numbers when phorate was applied to South Dakota wetlands [73]. Usually birds die when their brain AChE depression is over 75% [92, 114]. Thus, 11 out of 15 blue jays (Cyanocitta cristata) which had depression levels ranging 32-72% after disulfoton was sprayed to pecan groves would die [302], but their carcasses would probably not be found. In orchards sprayed with methomyl, oxamyl or dimethoate, the daily survival rates for nests of Pennsylvania mourning dove (Zenaida macroura) and American robin (Turdus migratorius) were significantly lower than in non-treated orchards, and the species diversity was also lower. Repeated applications of these and other insecticides reduced the reproductive success of doves and robins and may have lowered avian species diversity [93].
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Secondary poisoning with bendiocarb was attributed to 22 birds that had depressed AChE activity after eating contaminated mole crickets and other soil organisms on the applied turfgrass [224]. Several species of raptors were killed or debilitated after consuming waterfowl contaminated with phorate – the fowl had ingested granules of the insecticide that were applied to potato fields a few months earlier [84]. Equally, ladybugs (Hippodamia undecimnotata) fed upon Aphis fabae, which were reared on bean plants treated with carbofuran, experienced a 67% population reduction due to secondary poisoning [206]. Pirimicarb caused 30-40% mortality of Tasmanian brown lacewing (Micromus tasmaniae) larvae when feeding on contaminated 1st instar lettuce aphid (Nasonovia ribisnigri) for three days [298].
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Impacts on aquatic organisms usually do not last more than a month. For example, thiodicarb applied at 0.25-1.0 kg/ha had severe impacts on copepods, mayflies and chironomids in experimental ponds for three weeks, but not so much on aquatic beetle’s larvae; eventually there was recovery of all populations [7]. Pirimicarb can be lethal to common frog (Rana temporaria) tadpoles, but does not appear to have chronic effects [139]. However, vamidothion and acephate are most lethal to non-target organisms in rice crops, and are not recommended in IPM programs [153]. Carbofuran and phorate are very toxic to aquatic invertebrates [140], particularly amphipods and chironomids but not so much to snails, leeches or ostracods [72, 249]. Small negative effects in zooplankton communities (cladocerans copepods and rotifers) were observed in rice paddies treated with carbofuran at recommended application rates, but fish were not affected [107]. Carbofuran should not be used in rice paddies, whether in foliar or granular formulations: not only induces resurgence of the brown planthoppers (Nilaparvata lugens) [122], but it is also more toxic to the freshwater flagellate Euglena gracilis than the non-systemic malation [15]. It reduces populations of coccinellid beetles, carabid beetles, dragonfly and damselfly nymphs, but does not impact much on spiders [255]. However, it appears that carbofuran at 0.2% per ha can double the densities of Stenocypris major ostracods in rice paddies, whereas other insecticides had negative effects on this species [168]. Repeated applications of carbofuran can also have a significant stimulation of the rhizosphere associated nitrogenase activity, with populations of nitrogen-fixing Azospirillum sp., Azotobacter sp. and anaerobic nitrogen-fixing bacteria increasing progressively up to the third application of this insecticide [142].
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4.1.2. Insecticides acting on nAChR
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Direct toxicity of cartap to fish species is not as high as that of other neurotoxic insecticides, with 3-h LC50s between 0.02 and 6.8 mg/L [161, 308]. However, cartap affects negatively several species of Hymenoptera and aphid parasitoids used to control a number of crop pests [14, 77, 147, 270]. This insecticide also inhibits hatching of eggs of the nematode Agamermis unka, a parasite of the rice pest Nilaparvata lugens [50], and reduces significantly the populations of ladybugs and other predatory insects in cotton crops when applied at the recommended rates, i.e. 20 g/ha [109, 169]. In rice paddies, cartap hydrochloride reduced populations of coccinellid beetles, carabid beetles, dragonflies and damselflies by 20-50% [255]. Pollinators such as honey bees and bumble bees can also be seriously reduced in numbers when feeding on crops treated with cartap hydrochloride, which is included among the most toxic insecticides to bees after neonicotinoids and pyrethroids [179, 278]. For all its negative impacts on parasitoids and predatory insects it is hard to understand why cartap was the third most common insecticide (19% of all applications) used in IPM programs in Vietnam a decade ago [31], and is still among the most widely used in rice farms in China [308].
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Cumulative toxicity of neonicotinoids over time of exposure results in long-term pest control compared to the impact of cholinesterase inhibitor insecticides. For example, soil treated with clothianidin at 0.05-0.15% caused increasing mortality in several species of wireworms (Coleoptera: Elateridae), reaching 30-65% after 70 days, whereas chlorpyrifos at 0.15% produced 35% mortality within 30 days but no more afterwards [292]. Soil application of imidacloprid did not eliminate rapidly Asian citrus psyllid (Diaphorina citri) and leafminer (Phyllocnistis citrella) populations, but resulted in chronic residues in leaf tissue and long-term suppression of both pests [245]. Also, soil applications of neonicotinoids are very effective in controlling soil grubs and berry moths (Paralobesia viteana) in vineyards provided there is no irrigation or rain that washes off the insecticide [289]. For the same reason, however, the impact of neonicotinoids on non-target organisms is long-lasting. For example, repeated corn-seed treatement with imidacloprid caused a significant reduction in species richness of rove beetles in three years, even though the abundance of the main species was not affected [88]. In addition to long-term toxicity, acute toxicity of acetamiprid, imidacloprid and thiomethoxam to planthopper and aphid species is similar to that of synthetic pyrethroids, and higher than that of endosulfan or acetylcholinesterease inhibitors [219, 246]. Thus, combinations of pyrethroid-neonicotinoid have been hailed as the panacea for most pest problems as it suppresses all insect resistance [70]. Mixtures of imidacloprid and thiacloprid had additive effects on the toxicity to the nematode Caenorhabditis elegans but not on the earthworm Eisenia fetida [108].
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Acute toxicity of imidacloprid, thiamethoxam, clothianidin, dinotefuran and nitenpyram to honey bees is higher than that of pyrethroids, while toxicity of acetamiprid and thiacloprid is increased by synergism with ergosterol-inhibiting fungicides [134, 242] and antibiotics [116]. Thus, neonicotinoids can pose a high risk to honey bees, bumble bees [176, 263] and wasps [90]. Bees can be killed immediately by direct contact with neonicotinoid droplets ejected from seed drilling machines. Thus, numerous worker bees were killed when seed was coated with clothianidin during drilling of corn in the Upper Rhine Valley (Germany) in spring 2008 [102]. The same problem happened in Italy with thiamethoxam, imidacloprid and clothianidin [105, 285], leading to the banning of this application method on sunflower, canola and corn during 2008-09 [20]. However, most of the time bee colonies are intoxicated by feeding on contaminated pollen and nectar [9, 228]. It has been observed that bee foraging was notably reduced when Indian mustard was treated with 178 mg/ha imidacloprid [10]. Imidacloprid residues in sunflowers are below the no-adverse-effect concentration to honey bees of 20 μg/kg at 48-h [241], with surveys in France showing residue levels in pollen from treated crops in the range 0.1-10 μg/kg and average in nectar of 1.9 μg/kg [33]. However, bees feeding on such contaminated pollen or nectar will reach first sublethal and later lethal levels, with 50% mortality occurring within 1-2 weeks [228, 266]. Such data was disputed [89, 240] as it was in conflict with some long-term field observations of honey bees feeding on sunflowers grown from imidacloprid-treated seeds at 0.24 mg/seed [256]. However, recent evidence suggest that chronic lethality by imidacloprid is implicated in the colony collapse disorder (CCD) that affects honey bees [174]. Based on the fast degradation of imidacloprid in bees (4-5 hours), it is assumed that honey bees which consume higher amounts of imidacloprid die already outside of the hive, before the colony’s demise and before samples are taken, though residues of imidacloprid in bees at 5-8 μg/kg have been found in some cases [111]. Clothianidin residues of 6 μg/kg in pollen from canola fields reduced the number of bumble bee (Bombus impatients) workers slightly (~20%) [96], but exposure to clothianidin-treated canola for three weeks appeared not to have affected honey bee colonies in Canada [61]. Thiamethoxam applied to tomatoes (~150 g/ha) through irrigation water does not have impacts on bumble bees (Bombus terrestris) [244], whereas pollen contaminated with this insecticide causes high mortality and homing failure [125].
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Negative impacts of neonicotinoids on non-target soil arthropods are well documented. A single imidacloprid application to soil reduced the abundance of soil mesofauna as well as predation on eggs of Japanese beetle (Popillia japonica) by 28-76%, with impacts lasting four weeks. The same level of impact was observed with single applications of clothianidin, dinotefuran and thiamethoxam, so the intended pest control at the time of beetle oviposition runs into conflict with unintended effects – disruption of egg predation by non-target predators [210]. Among several insecticides applied to home lawns, only imidacloprid suppressed the abundance of Collembola, Thysanoptera and Coleoptera adults, non-oribatid mites, Hymenoptera, Hemiptera, Coleoptera larvae or Diptera taxonomic groups by 54-62% [209]. Imidacloprid applied to the root of eggplants (10 mg/plant) greatly reduced most arthropod communities and the species diversity during the first month. Small amounts of soil residues that moved into the surrounding pasture affected also some species; however, non-target ground arthropods both inside and outside the crop showed significant impacts only in the two weeks after planting [238], probably due to compensatory immigration from nearby grounds.
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Foliar applications of thiamethoxam and imidacloprid on soybean crops are preferred to seed treatments, as neonicotinoids appear to have lesser impacts on non-target communities than pyrethroids [204]. However, a foliar application of thiacloprid (0.2 kg/ha) to apple trees reduced the population of earwigs (Forficula auricularia), an important predator of psyllids and woolly apple aphid, by 60% in two weeks, while remaining below 50% after six weeks [294]. Branchlets of hemlock (Tsuga canadensis) treated with systemic imidacloprid (1-100 mg/kg) reduced the populations of two non-target predators of the hemlock woolly adelgid (Adelges tsugae) and had both lethal and sublethal effects on them [78]. Clothianidin, thiamethoxam and acetamiprid were as damaging to cotton crop predators as other broad-spectrum insecticides and cartap [169]. All neonicotinoids are lethal to the predatory mirid Pilophorus typicus, a biological control agent against the whitefly Bemisia tabaci, since their residual activity can last for 35 days on the treated plants [201]. The ladybug Serangium japonicum, also a predator of the whitefly, is killed in large numbers when exposed to residues of imidacloprid on cotton leaves applied at the recommended rate (40 ppm) or lower; apparently, the predator was not affected when imidacloprid was applied as systemic insecticide [120]. Clothianidin is 35 times more toxic to the predatory green miridbug (Cyrtorhinus lividipennis 48-h LC50 = 6 μg/L) than to the main pest of rice (Nilaparvata lugens 48-h LC50 = 211 μg/L), thus questioning seriously its application in such crops [221]. Not surprisingly, populations of predatory miridbugs and spiders suffered an initial set back when rice paddies were treated with a mixture of ethiprole+imidacloprid (125 g/ha), and their recovery was slow and never attained the densities of the control plots [154]. Mixtures of ethiprole+imidacloprid and thiamethoxam+l λ-cyhalothrin on rice paddies are also highly toxic to mirid and veliid natural enemies of rice pests, with 100% mortalities recorded in 24 h [159].
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Secondary poisoning with neonicotinoids reduces or eliminates eventually all predatory ladybirds in the treated areas, compromising biological control in IPM programs. Indeed, exposure of larval stages of Adalia bipunctata to imidacloprid, thiamethoxam, and acetamiprid, and adult stages to imidacloprid and thiamethoxam, significantly reduced all the demographic parameters in comparison with a control –except for the mean generation time–, thus resulting in a reduced coccinellid population; adult exposures produced a significant population delay [162]. Eighty percent of 3rd and 4th instar larvae of the ladybug Harmonia axyridis died after feeding for 6 hours on corn seedlings grown from seeds treated with clothianidin, compared to 53% mortality caused by a similar treatment with thiamethoxam; recovery occurred only in 7% of cases [196]. Survival of the ladybird Coleomegilla maculata among flower plants treated with imidacloprid at the label rate was reduced by 62% [251], and Hippodamia undecimnotata fed upon aphids reared on bean plants treated with imidacloprid, experienced a 52% population reduction [206]. Equally, 96% of Tasmanian brown lacewing (Micromus tasmaniae) larvae died after feeding on 1st instar lettuce aphid (Nasonovia ribisnigri) for three days. Low doses did not increase mortality but from days 3 to 8, lacewing larvae showed significant evidence of delayed developmental rate into pupae [298]. Grafton-Cardwell and Wu [110] demonstrated that IGRs, neonicotinoid insecticides, and pyrethroid insecticides have a significant, negative impact on vedalia beetles (Rodolia cardinalis), which are essential to control scale pests in citrus; neonicotinoids were toxic to vedalia larvae feeding on cottony cushion scale that had ingested these insecticides, and survival of adult beetles was also affected but to a lesser extent than other insecticides.
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Recent evidence of the negative impacts of neonicotinoids on parasitoids reinforces that these insecticides are not suitable for IPM [271]. All neonicotinoids are deadly to three whitefly parasitoid species (Eretmocerus spp. and Encarsia formosa), with mortality of adults usually greater than the pupae [267]. Thiamethoxam appears to be less toxic to whitefly parasitoids compared to imidacloprid [202]. Imidacloprid, thiamethoxam and nitenpyram appeared to be the most toxic to the egg parasitoids Trichogramma spp. [231, 299]. For example, the acute toxicity of thiomethoxam and imidacloprid to Trichogramma chilonis, an egg parasitoid of leaf folders widely used in cotton IPM, is about 2000 times higher than that of other insecticides used in rice crops in India, such as acephate or endosulfan [220]. Acute toxicity of imidacloprid is more pronounced on Braconidae parasitoids than on T. chilonis, whereas thiacloprid only reduced the parasitization on Microplitis mediator [192]. Thiacloprid is as toxic to the cabbage aphid Brevicoryne brassicae as to its parasitoid (Diaeretiella rapae), whereas pirimicarb and cypermethrin are more toxic to the aphid and are, therefore, preferred in IPM [3].
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Neonicotinoids pose also risks to aquatic taxa. The synergistic toxicity of imidacloprid+thiacloprid on Daphnia magna [173] implies the combined effect of neonicotinoids on aquatic arthropods would be higher than expected, even if Daphnia is very tolerant of neonicotinoids [119]. Other contaminants, such as the nonylphenol polyethoxylate (R-11) act also synergistically with imidacloprid [49]. Thiacloprid causes delayed lethal and sublethal effects in aquatic arthropods, which can be observed after 4 to 12 d following exposure to single 24-h pulses [28]. Thus, its 5% hazardous concentration (0.72 μg/L) is one order of magnitude lower than predicted environmental concentrations in water [35]. Also, thiacloprid LC50 for survival of midges (Chironomus riparius) is only 1.6 μg/L, and EC50 for emergence 0.54 μg/L [160], so both acute and chronic toxicity reduce the survival and growth of C. tentans and Hyalella azteca [265]. Acute toxicity of neonicotinoids to red swamp crayfish (Procambarus clarkii) is 2-3 orders of magnitude lower than that of pyrethroids [23]; comparative data such as this gives the neonicotinoids an apparent better environmental profile. However, experimental rice mesocosms treated with imidacloprid at label rates (15 kg/ha) eliminated all zooplankton communities for two months, and their recovery did not reach the control population levels four months later. Equally, mayflies, coleoptera larvae and dragonfly nymphs were significantly reduced while residues of imidacloprid in water were above 1 μg/L [117, 237]. Similarly, streams contaminated with a pulse of thiacloprid (0.1-100 μg/L) resulted in long-term (7 months) alteration of the overall invertebrate community structure [27]. However, while aquatic arthropods with low sensitivity to thiacloprid showed only transient effects at 100 μg/L, the most sensitive univoltine species were affected at 0.1 μg/L and did not recover during one year [167].
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4.1.3. Fipronil
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Fipronil is very efficient in controlling locust outbreaks, but causes more hazards than chlorpyrifos and deltamethrin to non-target insects in the sprayed areas, although it is more selective to specific taxa [214, 252]. Thus, abundance, diversity and activity of termites and ants were all reduced in northern Australia after spraying several areas with fipronil for locust control [262], and 45% of the termite colonies died within 10 months of a spraying operation with fipronil for controlling locusts in Madagascar [214]. Reducing the recommended application rates by seven times (0.6-2 g/ha) still achieves 91% elimination of locusts while having lesser impacts on non-target organisms, comparable to those inflicted by carbamate and OP insecticides [18].
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Despite its selectivity, fipronil in maize crops reduced the abundance of arthropod populations of the soil mesofauna more significantly than other systemic insecticides, i.e. carbofuran [59], although springtails are little affected as they avoid feeding on litter contaminated with fipronil and are more tolerant of this insecticide [232]. When applied to citrus orchards, fipronil was among the most detrimental insecticides affecting two Euseius spp. of predatory mites [112]. In rice crops, the effectiveness of fipronil in controlling pests was overshadowed by its negative impact on the predatory miridbugs Cyrtorhinus lividipennis and Tytthus parviceps [159].
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Of greater concern is the impact of this systemic chemical on honey bees and wild bee pollinators. With an acute contact LD50 of 3.5 ng/bee [166] and acute oral LD50 of 3.7-6.0 ng/bee [2], fipronil is among the most toxic insecticides to bees ever developed. Even more worrying is the finding that the adjuvant Sylgard, used to reduce the toxicity of most insecticidal products on bees, increases the toxic effects of fipronil [184]. The systemic nature of this chemical implies that chronic feeding of the bees on nectar contaminated with fipronil caused 100% honey bee mortality after 7 days, even if the residue concentration was about 50 times lower than the acute lethal dose [8]. Residues of fipronil in pollen have been measured as 0.3-0.4 ng/g, which are 30-40 times higher than the concentration inducing significant mortality of bees by chronic intoxication [33]. Unlike neonicotinoids, no residues of fipronil have been found in guttation drops [272].
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The acute toxicity of fipronil to cladocerans is similar to the toxicity to estuarine copepods, with 48-h LC50 in the range 3.5-15.6 μg/L [47, 259], but the chronic toxicity with time of exposure is what determines the fate of the populations exposed. For example, populations of Daphnia pulex went to extinction after exposure to 80 μg/L for 10 days, equivalent to LC75 [259], and 40% of a population of grass shrimps (Palaemonetes pugio) died in 28 days after being exposed to fipronil concentrations of 0.35 μg/L in marsh mesocosms, and none of the shrimps survived when exposed to 5 mg/L during the same period [303]. Such impacts on zooplankton are likely to occur in estuaries, where waters have been found to contain 0.2-16 μg/L of fipronil residues [45, 163], even if no apparent effect on amphipods, mussels nor fish has been observed [37, 303]. Fipronil sprays on water surfaces to control mosquito larvae have negative impacts not only on cladocerans but also on chironomid larvae exposed to chronic feeding on contaminated residues [183, 264]. Studies on rice mesocosms have shown that significant population reductions due to fipronil application at the recommended rates (50 g per seedling box) are not restricted to zooplankton and benthic species, but affect most species of aquatic insects. Moreover, fipronil impacts on aquatic arthropods were more pronounced after a second application in the following year [118], indicating persistence of this insecticide in rice paddies. Chronic toxicity over time explains the long-term toxicity of this systemic compound, so it is not surprising that concentrations of 1.3 μg/L in paddy water were sufficient to kill 100% of dragonfly (Sympetrum infuscatum) nymphs in nine days [138].
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4.1.4. Insect growth regulators
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There is little information about the effect of systemic chitin inhibitors on non-target organisms. Obviously these compounds are harmless to fish at levels above 1 mg/L for a week-long exposures [290], and to all vertebrates in general. IGRs affect mainly the larval stages of Lepidoptera, Coleoptera and Hymenoptera, and their activity last longer than that of other pest control products [178]. The effectiveness of these compounds in controlling target pests is demonstrated by comparing the dietary LC50 of hexaflumuron (0.31 mg/L) to the target cotton worm (Helicoverpa sp.), which is 35 times lower than that of the systemic carbamate thiodicarb and less damaging to non-target predators [64]. Aquatic communities of non-target arthropods in rice fields (e.g. Cladocera, Copepoda, Odonata, Notonectidae, Coleoptera and Chironomidae taxa) were not affected by teflubenzuron applied at rates to control mosquitoes (5.6 mg/ha), even though this IGR remained active for several weeks during autumn and winter periods [239].
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After application of IGRs to a crop, affected insect pests are prey to many species of spiders, some of which are also susceptible to the toxicity of these products, in particular the ground hunter spiders [211]. Larvae and eggs of pests contaminated with systemic IGR are consumed by a number of predators, including earwigs, which undergo secondary poisoning and stop growing beyond the nymph stage [226]. Chitin inhibitors only show effects on the larvae of predatory insects that had consumed treated-prey, not on the adult insects. As a consequence, predatory populations collapse, as it happened with the ladybeetle Chilocorus nigritus that fed on citrus red scales (Aonidiella aurantii) in African orchards that had been treated with teflubenzuron [177]. Teflubenzuron sprayed at 16.4 g/ha for locust control in Mali did not affect the non-target arthropods in the herb layer, whereas ground-living Collembola, Thysanura, Coleoptera and Lepidoptera larvae were reduced by about 50% [151]. Moreover, teflubenzuron has multigenerational impacts: experiments with springtails exposed to artificial soil contaminated with this IGR showed that the F2 generation suffered significantly from its effects even when only the F0 generation had been exposed for 10 days [42]. Secondary poisoning with chitin inhibitors can be detrimental also to parasitoids such as Diadegma semiclausum, which may fail to produce enough cocoons in the treated hosts, but do not seem to affect the parasitism of other Hymenoptera [98]. For instance, novaluron did not affect the parasitisation of Trichogramma pretiosum on mill moth’s caterpillars, a pest of tomato crops [44]. On the other hand, teflubenzuron appears to be harmless to predatory mites [32]. IPM programs must always consider the implications of using systemic chitin inhibitors to control specific pests without destroying their natural predators in the first place.
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Halofenozide does not appear to cause any acute, adverse effects through topical, residual, or dietary exposure of the ground beetle Harpalus pennsylvanicus. In contrast to the negative effects of other systemic insecticides (i.e. imidacloprid), the viability of eggs laid by females fed halofenozide-treated food once, or continuously for 30 days, was not reduced [156].
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4.2. Sublethal effects
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Very often, sublethal effects of systemic insecticides are a first step towards mortality, as they are caused by the same neurotoxic mechanisms. Apart from these, there may be other effects on reproduction, growth, longevity, etc. when organisms are exposed to low, sublethal doses or concentrations. These effects are only observable in individuals that survive the initial exposure, or in species that are tolerant to insecticides. For a review see [69].
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4.2.1. Acetylcholinesterase inhibitors
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Longevity of the parasitoid Microplitis croceipes that fed on nectar from cotton treated with aldicarb was affected for at least 10 days after application, and its foraging ability of the parasitoid’s host was severely impaired for 18 days [257]. Carbofuran caused a significant reduction of adult weight and longevity of the predator ladybug Hippodamia undecimnotata, as well as a 55% reduction in fecundity when fed on aphids contaminated with this insecticide [206]. Longevity and survival of Aphidius ervi, an important parasitoid of the pea aphid (Acyrthosiphon pisum), were significantly reduced after treating with LC25 concentrations of dimethoate or pirimicarb [11]. A significant reduction in body size of females of the predator carabid Pterostichus melas italicus and altered sexual dimorphism were observed after long-term exposure in olives groves treated with dimethoate at a rate that caused 10% mortality after three days [104]. Unlike other insecticides, no behavioural effects of dimethoate or triazamate on honey bees were recorded [67].
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Earthworms (Lumbricus terrestris) experienced significant reduction in growth rate and total protein content after soil applications of aldicarb at LC10 or LC25, but only small amounts of residues were detected in the worms [198]. Aldicarb and phorate can also increase infections by Rhizoctonia stem canker in potato fields [280].
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A typical pattern of sublethal intoxication was revealed when red-winged blackbirds (Agelaius phoeniceus) were exposed to increasing doses of dimethoate: 2 mg/kg b.w. doses produced ataraxia, defecation and diarrhoea; neuromuscular dysfunctions and breathing complications appeared at 3 mg/kg, and by 5 mg/kg muscle paralysis and death occurred. The estimated LC50 was 9.9 mg/kg, and all birds died at doses above 28 mg/kg [38]. Although sublethal AChE depression by acephate (25% brain) did not affect the attack behaviour in American kestrels (Falco sparverius) [229], nor did alter breeding behaviour in American robins (Turdus migratorius) [65], exposure to 256 mg/kg b.w. acephate impaired the migratory orientation of the white-throated sparrow (Zonotrichia albicollis) [295]. Similarly, low doses of demeton–S-methyl did not affect starlings (Sturnus vulgaris) behaviour [279], but doses of 2.5 mg/kg b.w. of dicrotophos administered to female starlings significantly reduced their parental care and feeding of nestlings [113]. Carbofuran orally administered to pigeons (Columba livia) had profound effects on flight time, with pigeons falling off the pace of the flock when doses were between 0.5 and 1.0 mg/kg b.w. [36].
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AChE activities in adductor muscle were depressed in freshwater mussels (Elliptio complanata) exposed for 96 h at concentrations as low as 0.1 mg/L and 1.3 mg/L of aldicarb and acephate respectively, while increasing the water temperature from 21 to 30 oC resulted in mortality [199]. High AChE inhibition (70%) by acephate was not associated with immobility of Daphnia magna, but increasing the concentration of acephate further had a strong detrimental effect on mobility, suggesting that binding sites other than AChE may be involved in acephate toxicity [222].
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Exposure of bluegill fish (Lepomis macrochirus) to 30 μg/L carbofuran decreased significantly adenylate parameters in gill, liver, muscle and stomach tissues after 10 days, and then returned to normal [128]. Also, concentrations of carbofuran at half the LC50 dose for fathead minnow (Pimephales promelas) larvae caused reductions in swimming capacity, increased sensitivity to electric shocks, and a reduction in upper lethal temperature [121]. Enzymes of protein and carbohydrate metabolism were altered (some increased, others decreased) in liver and muscle tissues of the freshwater fish, Clarias batrachus when exposed to 7.7 mg/L of carbofuran for six days, recovering later to normal levels [26]. Exposure of guppies (Brachydanio rerio) to half the recommended dose for dimethoate (0.025 μl/L) caused morphological changes in hepatocytes within three days, as well as necrosis and other abnormalities [227]. When exposed to a range of monocrotophos concentrations (0.01-1.0 mg/L), male goldfish (Carassius auratus) showed higher levels of 17-β-estradiol and vitollogenin and lower levels of testosterone than normal, interfering with gonadotropin synthesis at the pituitary gland [281]. Eggs of the toad Bufo melanostictus exposed to acephate hatched normally, but the tadpoles exhibited deformities such as tail distortions and crooked trunk; decreased pigmentation, peeling of the skin, inactivity, delay in emergence of limbs and completion of metamorphosis were also apparent [103].
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Insecticide mixtures can enhance not only the acute but also the sublethal effects. For example, disulfoton together with endosulfan caused cytological and biochemical changes in liver of rainbow trout (Oncorrhynchus mykiss), independently of their respective modes of action [13]. Mixtures of aldicarb and other insecticides enhanced significantly the establishment of parasitic lungworm nematodes (Rhandias ranae) in leopard frogs (Rana pipiens) some 21 days after infection [101], as the frog’s immune response was suppressed or altered [51]. Similarly, laboratory rats exposed to sublethal mixtures of aldicarb, methomyl and a herbicide (metribuzin) showed learning impairment, immune response and endocrine changes [215].
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4.2.2. Insecticides acting on nAChR
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Laboratory experiments have shown a number of abnormalities such as less melanin pigmentation, wavy notochord, crooked trunk, fuzzy somites, neurogenesis defects and vasculature defects in zebrafish (Danio rerio) embryos exposed to a range of cartap concentrations. The most sensitive organ was the notochord, which displayed defects at concentrations as low as 25 μg/L [308]. It is obvious that essential enzymatic processes are disturbed during embryo development, among which the inhibition of lysyl oxidase is responsible for the notochord undulations observed.
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Imidacloprid does not cause high mortality among eggs or adults of the preparasite nematode Agamermis unka, but impairs the ability of the nematode to infect nymphs of the host brown planthopper (Nilaparvata lugens) [50]. Contrary to this, a synergistic effect of imidacloprid on reproduction of entomopathogenic nematodes against scarab grubs may increase the likelihood of infection by subsequent generations of nematodes, thereby improving their field persistence and biological potential to control grubs. Acetamiprid and thiamethoxam, however, do not show synergist interactions with nematodes [149]. Imidacloprid at 0.1-0.5 mg/kg dry soil disturbs the burrowing ability of Allolobophora spp. earthworms [43], and the highest concentration can also induce sperm deformities in the earthworm Eisenia fetida [306]. Reduction in body mass (7-39%) and cast production (42-97%) in Allolobophora spp. and Lumbricus terrestris have also been observed after 7 days exposure to relevant environmental concentrations of imidacloprid [74]. Residues of imidacloprid in maple leaves from treated forests (3–11 mg/kg) did not affect survival of aquatic leaf-shredding insects or litter-dwelling earthworms. However, feeding rates by aquatic insects and earthworms were reduced, leaf decomposition (mass loss) was decreased, measurable weight losses occurred among earthworms, and aquatic and terrestrial microbial decomposition activity was significantly inhibited, thus reducing the natural decomposition processes in aquatic and terrestrial environments [150].
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The dispersal ability of the seven-spotted ladybirds (Coccinella septempunctata) sprayed with imidacloprid was compromised, and this may have critical consequences for biological control in IPM schemes [21]. A significant reduction of adult weight and longevity of the ladybug Hippodamia undecimnotata, as well as 33% reduction in fecundity were observed when this predatory bug fed on aphids contaminated with imidacloprid [206]. Imidacloprid and fipronil had adverse effects on the immune response of the wolf-spider Pardosa pseudoannulata, reducing significantly its phenoloxidase activity, the total number of hemocytes and encapsulation rate [282]; the implications of such effects on this natural enemy of rice pests are unknown. When applied in the egg-larval or pupal stages, acetamiprid or imidacloprid reduced the parasitisation capacity of F1 and F2 generation females of Trichogramma pretiosum on mill moth’s caterpillars (Anagasta kuehniella), a pest of tomato crops [44]. Longevity of females of the parasitoid Microplitis croceipes that fed on nectar from imidacloprid-treated cotton was affected for at least 10 days after application, while the parasitoid\'s host foraging ability was severely affected from day 2 onwards [257]. Exposure of western subterranean termites (Reticulitermes hesperus) to acetamiprid (1 mg/kg sand) or imidacloprid also impaired locomotion of termites within 1 hour [230].
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Bumble bees (Bombus terrestris) interrupt their activity for several hours when exposed to imidacloprid sprayed on plants [132], and soil treatment at the highest recommended doses extended the handling times of B. impatiens on the complex flowers [194]. Such an impairment affects the bees foraging behaviour and can result in a decreased pollination, lower reproduction and finally in colony mortality due to a lack of food [193]. Although Franklin et al. [96] found that clothianidin residues of 6 μg/kg in canola pollen reduced the production of queens and increased the number of males in B. impatients, their study did not find significant differences with controls due to a high variability in the results. Larval development in wild bees (Osmia lignaria and Megachile rotundata) was delayed significantly when fed pollen contaminated with either imidacloprid or clothianidin at 30 or 300 μg/kg [1]. Honey bees are more sensitive to neonicotinoids than bumble bees: at 6 μg/kg, imidacloprid clearly induced a decrease in the proportion of active bees [57], and 50-500 μg/L affect significantly their activity, with bees spending more time near the food source [273]. Other authors found that lower activity of honey bees during the hours following oral exposure to 100-500 μg/L imidacloprid in syrup is transitory [186]. In any case, that may explain the delayed homing behaviour of honey bees exposed to 100 μg/L imidacloprid in syrup and their disappearance at higher doses [34, 304]. Honey bees fed on syrup contaminated with acetamiprid increased their sensitivity to antennal stimulation by sucrose solutions at doses of 1 μg/bee and had impaired long-term retention of olfactory learning at 0.1 μg/bee. Contact exposure at 0.1 and 0.5 μg/bee increased locomotor activity and water-induced proboscis extension reflex but had no effect on behaviour [82]. Similar response was obtained with honey bees exposed to thiomethoxam by contact, having impaired long-term retention of olfactory learning at 1 ng/bee [8]. Winter bees surviving chronic treatment with imidacloprid and its metabolite (5-OH-imidacloprid) had reduced learning performances than in summer: the lowest-effect concentration of imidacloprid was lower in summer bees (12 μg/kg) than in winter bees (48 μg/kg), indicating a greater sensitivity of honey bees behaviour in summer bees compared to winter bees [68].
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Honey bees infected with the microsporidian Nosema ceranae experienced 7 or 5 times higher mortality than normal when fed syrup contaminated with sublethal doses of thiacloprid (5 mg/L) or fipronil (1 μg/L), respectively [293). N. ceranae is a key factor in the CCD in honey bees [127], and the synergistic effect of these systemic insecticides on Nosema is probably its underlying cause [213]. Suppression of the immune system is not restricted to bees, as a massive infection of medaka fish by a protozoan ectoparasite (Trichodina spp.) when exposed to imidacloprid in rice mesocosms has been documented [236].
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Imidacloprid residues in water as low as 0.1 μg/L are sufficient to reduce head and torax length in mayfly nymphs of Baetis and Epeorus, whether applied as pulses or in continuous exposures for 20 days [6]. At 1 μg/L the insecticides caused feeding inhibition. However, 12-h pulses induced emergence because of stress, whereas constant exposure reduced survivorship progressively. Also, the aquatic worm Lumbriculus variegatus experienced immobility during 4 days when exposed to 0.1-10 μg/L imidacloprid [5].
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4.2.3. Fipronil
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Apart from the extreme acute toxicity of this insecticide to bees, honey bees fed on sucrose syrup contaminated with fipronil (2 μg/kg) reduced significantly their attendance to the feeder [57]. It has also been demonstrated that sublethal concentrations of this insecticide as low as 0.5 ng/bee, whether orally or topically applied, reduce the learning performance of honey bees and impair their olfactory memory but not their locomotor activity [67, 82]. Furthermore, chronic feeding exposure at 1 μg/kg or 0.01 ng/bee reduced learning and orientation, whilst oral treatment of 0.3 ng/bee reduced the number of foraging trips among the exposed workers [66]. In addition to their activity, honey bees fed with sucrose syrup containing 1 μg/L fipronil increased significantly the mortality of bees infected with the endoparasite Nosema ceranae, suggesting a synergistic effect between the insecticide and the pathogen [293]. All these sublethal effects reduce the performance of the hive and help explain the decline in honey bee and wild bee pollinators in many countries [205], although fipronil is not alone in causing this demise – neonicotinoids are equally implicated.
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Female zebra finches (Taeniopygia guttata) fed with single sublethal doses of fipronil (1, 5, and 10 mg/kg b.w.) failed to hatch 6 out of 7 eggs laid. The only chick born was underdeveloped and had fiprole residues in the brain, liver and adipose tissues. By contrast, 12-day-old chicken eggs injected with fipronil (5.5 to 37.5 mg/kg egg weight) hatched normally although the chicks from the highest dose group showed behavioural and developmental abnormalities [145].
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Low residues of fipronil in estuary waters (0.63 μg/L) inhibited reproduction of the copepod Amphiascus tenuiremis by 73-89%, and this effect seems to be more prevalent on males than on females [45]. Even lower residue levels (0.22 μg/L) halted egg extrusion by 71%, whereas exposure to 0.42 μg/L nearly eliminated reproduction (94% failure) on this species. Based on these results from chronic and sublethal toxicity, a three-generation Leslie matrix model predicted a 62% decline in population size of A. tenuiremis at only 0.16 μg/L [47]. Unlike other insecticides, the stress on Ceriodaphnia dubia caused by predatory cues of bluegill fish (Lepomis macrochirus) was significantly exacerbated when the cladocerans were exposed to 80-160 μg/L of fipronil [223]; however, these concentrations are much higher than the residue levels usually found in waters [99, 163].
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While fipronil applied at the recommended rates in rice fields induces biochemical alterations in carp (Cyprinus carpio), such metabolic disturbances do not appear to have any effect on growth nor mortality of this fish after 90 days exposure at <0.65 μg/L [52]. However, similar residue levels (<1 μg/L) reduced significantly the growth of adult medaka fish (Oryzias latipes) after two weeks of exposure, as well as growth of their offspring in the first 35 days, even if residues of fipronil by that time were below the analytical detection limit (0.01 μg/L) [117].
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4.2.4. Insect growth regulators
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Longevity of predatory bug Podisus maculiventris was reduced after preying on Colorado potato beetles that fed on foliage treated with novaluron at 85 g/ha. Females produced fewer eggs and their hatching was significantly suppressed, while 5th instars that also preyed on the beetles failed to moult into adults [62]. Novaluron and hexaflumuron significantly decrease (<30%) the total protists population in the guts of termites (Reticultermes flavipes), thus upsetting their digestive homeostasis [165].
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4.3. Indirect effects on populations and communities
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Indirect effects result from the dynamics of ecosystems. Thus, applications of granular phorate to soil eliminate most soil invertebrates (see 4.1) except for Enchytraeidae worms, which increase in large numbers and take over the leaf-litter decomposition function carried out by the eliminated springtails [300].
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Resurgence or induction of pests by altering the prey-predator relationships in favour of the herbivore species is most common. When carbofuran was applied to corn plantations in Nicaragua, the population levels of the noctuid pest Spodoptera frugiperda increased because of lesser foraging activity by predatory ants [212]. Methomyl eliminated the phytoseiid predatory mite Metaseiulus occidentalis for 10 days, thus causing an increase in Pacific spider mites (Tetranychus pacificus) and leafhopper (Eotetranychus willamettei) populations in the treated vineyards [130]. Unexpected outbreaks of a formerly innocuous herbivore mite (Tetranychus schoenei) were observed after imidacloprid applications to elms in Central Park, New York. A three-year investigation on the outbreaks showed that elimination of its predators and the enhanced fecundity of T.\n\t\t\t\t\tschoenei by this insecticide were responsible for that outcome [268].
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The widespread use of insecticides usually tips the ecological balance in favour of herbivore species. For example, dimethoate sprayed on clover fields indirectly reduced the populations of house mice (Mus musculus) in the treated areas as the insect food source was depleted. However, herbivore species such as prairie voles (Microtus ochrogaster) and prairie deer mouse (Peromyscus maniculatus) increased in density levels [24], since they had more clover available due to either higher clover yields or through less competition with the house mice or both.
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A reduction in arthropod populations often implies starvation of insectivorous animals. For example, densities of two species of lizards and hedgehogs in Madagascar were reduced 45-53% after spraying with fipronil to control a locust outbreak, because their favourite termite prey was almost eliminated (80-91%) by this chemical [214]. However, this type of indirect impact is difficult to observe and measure in birds, since they can move to other areas or change their resource diet. For example, hemlock forests treated with imidacloprid to control hemlock woolly adelgid (Adelges tsugae) reduced significantly Hemiptera and larval Lepidoptera, but not other insect taxa. Although larval Lepidoptera are the primary prey for insectivorous foliage-gleaning birds, many birds were able to find other food resources in the mixed hemlock-deciduous stands that were not treated [87]. Similarly, post-treatment with fipronil for grasshopper control in Wyoming did not affect bird densities, perhaps due to the large initial insect populations; fipronil plots generally had higher avian population densities (nongregarious, insectivores and total birds) than other areas treated with carbaryl [203]. Although some early studies found that fipronil did not have much impact on aquatic communities of Sahelian ponds [158], nor in predatory invertebrates in the Camargue marshes, herons in the latter region avoid rice fields treated with fipronil because of the scarcity of invertebrate food in there [188].
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Food aversion to pesticide-treated seeds or plants is a mechanism that may indirectly ameliorate the toxic effects of systemic insecticides such as carbofuran in mice and other small rodents [170]. Some Collembola species (i.e. Folsomia fimetaria) avoid dimethoate sprayed areas [86], and female parasitoids (Cotesia vestalis) are discouraged from getting to their host –the diamond-back month (Plutella xylostella) – in turnip plants treated with methomyl, whereas clothianidin does not produce aversion [248]. Equally, dimethoate and oxydemeton-methyl sprayed on peach trees discourage honey bees from visiting in the first two days after application, while treatments with imidacloprid, acetamiprid and thiamethoxam allow honey bees visits [246]. This helps explain the high long-term impact of neonicotinoids on bees compared to the effect of OP insecticides, even if imidacloprid at high experimental concentrations in syrup (>0.5 mg/L) may also have repellent effect on honey bees [34].
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5. Risk assessment of systemic insecticides
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All systemic compounds have effects with time of exposure. However, only the persistent chemicals (fipronil, neonicotinoids, cartap and some OPs) have cumulative effects over time, since the non-persistent compounds are quickly degraded in soil and water.
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For risk assessment of these compounds it is important to understand their chronic impacts. Unlike traditional protocols based on acute toxicity, the persistent activity of the parent and toxic metabolites requires that exposure time must be taken into consideration [115]. Concerns about the impacts of dietary feeding on honey bees and other non-target organisms are thus justified [9, 60, 228], because the accumulation of small residue levels ingested repeatedly over time will eventually produce a delayed toxic effect [276]. For example, bees that feed on contaminated nectar and pollen from the treated crops are exposed to residues of imidacloprid and fipronil in the range 0.7-10 μg/kg and 0.3-0.4 μg/kg respectively [33], which appear in 11% and 48% of the pollen surveyed in France [48]. Based on those findings an estimate of the predicted environmental concentrations that bees are ingesting in that country can be made for each insecticide. Since there is a log-to-log linear relationship between concentration and time of exposure [234], the critical levels of residue and time of exposure can be determined.
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The declining populations of predatory and parasitic arthropods after exposure to recommended applications of most systemic insecticides are worrying. In view of the above, it not so much the small concentrations they are exposed to but the time of exposure that makes the population decline progressively over weeks, months and even years of treatment, as described in this chapter. Lethal and sublethal effects on reproduction are equally implicated. This is the reason why systemic insecticides should be evaluated very carefully before using them in IPM schemes. Obviously, recovery rates are essential for the populations affected to come back, and this usually occurs by recolonisation and immigration of individuals from non-affected areas. For example, modelling based on recovery data after dimethoate application to wheat fields [277] demonstrates that a non-target organism that is reduced by only 20% but is unable to recover is likely to be far more at risk from exposure to a pesticide than an organism that is reduced 99% for a short period but has a higher recovery potential.
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The above is also relevant to the impact of small residues of those systemic insecticides that have cumulative effects (e.g. neonicotinoids, fipronil and cartap) on aquatic ecosystems. Because of the short life-cycle of many zooplankton species, the negative population parameters that result from sublethal and chronic effects on such organisms can lead their local populations to extinction [260]. Immediate reductions in populations and species may not always be apparent due to the small residue concentrations and the delayed effects they cause. For example, in recent surveys of pesticide residues in freshwaters of six metropolitan areas of USA, fipronil appears regularly in certain states [254]. Fipronil and its desulfinyl, sulfide, and sulfone degradates were detected at low levels (≤ 0.18–16 μg/L) in estuary waters of Southern California [163], and make some 35% of the residues found in urban waters, with a median level of 0.2-0.44 μg/L, most frequently during the spring-summer season [99]. Imidacloprid was detected in 89% of water samples in agricultural areas of California, with 19% exceeding the US Environmental Protection Agency’s chronic invertebrate Aquatic Life Benchmark of 1.05 μg/L [261]. In the Netherlands, imidacloprid appeared in measurable quantities in 30% of the 4,852 water samples collected between 1998 and 2007 [287]. These figures indicate there is already a widespread contamination of waterways and estuaries with persistent systemic insecticides.
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The first consequence of such contamination is the progressive reduction, and possible elimination, of entire populations of aquatic arthropods from the affected areas. As time is a critical variable in this type of assessment, it is envisaged that should this contamination continue at the current pace over the years to come the biodiversity and functionality of many aquatic ecosystems will be seriously compromised [191]. Secondly, as these organisms are a primary food source of a large number of vertebrates (e.g. fish, frogs and birds), the depletion of their main food resource will inevitably have indirect impacts on the animal populations that depend on them for their own survival. The case of the partridge in England is an example of how a combination of herbicides and insecticides can bring the demise of a non-target species by indirectly suppressing its food requirements [217]. Therefore, warnings about the possible role of environmental contamination with neonicotinoids in steeply declining populations of birds, frogs, hedgehogs, bats and other insectivorous animals are not far fetched and should be taken seriously [275].
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6. Conclusions
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This review has brought some light on the direct, sublethal and indirect effects that systemic insecticides have on species populations and ecosystems. Some long-term impacts have been known for some time (e.g. carbofuran, phorate), but it is the rapid increase in the usage of neonicotinoids and other systemic products that poses a new challenge to the ecological risk assessment of agrochemicals. Indeed, current risk protocols, based on acute, short-term toxic effects are inadequate to cope with the chronic exposure and cumulative, delayed impacts of the new compounds. Awareness of the increasing contamination of the environment with active residues of these chemicals should help regulators and managers to implement new approaches for risk assessment of these substances.
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\n\t\n',keywords:null,chapterPDFUrl:"https://cdn.intechopen.com/pdfs/40497.pdf",chapterXML:"https://mts.intechopen.com/source/xml/40497.xml",downloadPdfUrl:"/chapter/pdf-download/40497",previewPdfUrl:"/chapter/pdf-preview/40497",totalDownloads:3731,totalViews:1299,totalCrossrefCites:10,totalDimensionsCites:38,totalAltmetricsMentions:0,impactScore:12,impactScorePercentile:98,impactScoreQuartile:4,hasAltmetrics:0,dateSubmitted:"May 3rd 2012",dateReviewed:"August 28th 2012",datePrePublished:null,datePublished:"January 30th 2013",dateFinished:"October 25th 2012",readingETA:"0",abstract:null,reviewType:"peer-reviewed",bibtexUrl:"/chapter/bibtex/40497",risUrl:"/chapter/ris/40497",book:{id:"3055",slug:"insecticides-development-of-safer-and-more-effective-technologies"},signatures:"Francisco Sánchez-Bayo, Henk A. Tennekes and Koichi Goka",authors:[{id:"74970",title:"Dr.",name:"Francisco",middleName:null,surname:"Sánchez-Bayo",fullName:"Francisco Sánchez-Bayo",slug:"francisco-sanchez-bayo",email:"sanchezbayo@mac.com",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:{name:"University of Sydney",institutionURL:null,country:{name:"Australia"}}}],sections:[{id:"sec_1",title:"1. Introduction",level:"1"},{id:"sec_2",title:"2. Exposure to systemic insecticides",level:"1"},{id:"sec_3",title:"3. Modes of action of systemic insecticides",level:"1"},{id:"sec_3_2",title:"3.1. Acetylcholinesterase inhibitors",level:"2"},{id:"sec_4_2",title:"3.2. Insecticides acting on nicotinic acetylcholine receptors (nAChR)",level:"2"},{id:"sec_5_2",title:"3.3. GABA-R antagonists (fipronil)",level:"2"},{id:"sec_6_2",title:"3.4. Insect growth regulators (IGR)",level:"2"},{id:"sec_8",title:"4. Effects on organisms and ecosystems",level:"1"},{id:"sec_8_2",title:"4.1. Direct effects on organisms",level:"2"},{id:"sec_8_3",title:"4.1.1. Acetylcholinesterase inhibitors",level:"3"},{id:"sec_9_3",title:"4.1.2. Insecticides acting on nAChR",level:"3"},{id:"sec_10_3",title:"4.1.3. Fipronil",level:"3"},{id:"sec_11_3",title:"4.1.4. Insect growth regulators",level:"3"},{id:"sec_13_2",title:"4.2. Sublethal effects",level:"2"},{id:"sec_13_3",title:"4.2.1. Acetylcholinesterase inhibitors ",level:"3"},{id:"sec_14_3",title:"4.2.2. Insecticides acting on nAChR",level:"3"},{id:"sec_15_3",title:"4.2.3. Fipronil",level:"3"},{id:"sec_15_4",title:"4.2.4. Insect growth regulators",level:"4"},{id:"sec_18_2",title:"4.3. Indirect effects on populations and communities",level:"2"},{id:"sec_20",title:"5. Risk assessment of systemic insecticides",level:"1"},{id:"sec_21",title:"6. Conclusions",level:"1"}],chapterReferences:[{id:"B1",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tAbbott\n\t\t\t\t\t\t\tV. 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M.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMoineau\n\t\t\t\t\t\t\tI.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tGaimon\n\t\t\t\t\t\t\tC.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tBrun\n\t\t\t\t\t\t\tS.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tVermandere\n\t\t\t\t\t\t\tJ. P.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2004\n\t\t\t\t\tA method to quantify and analyze the foraging activity of honey bees: relevance to the sublethal effects induced by systemic insecticides\n\t\t\t\t\tArch. Environ. Contam. Toxicol.\n\t\t\t\t\t47\n\t\t\t\t\t3\n\t\t\t\t\t387\n\t\t\t\t\t395\n\t\t\t\t\n\t\t\t'},{id:"B58",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tColinas\n\t\t\t\t\t\t\tC.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tIngham\n\t\t\t\t\t\t\tE.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMolina\n\t\t\t\t\t\t\tR.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t1994\n\t\t\t\t\tPopulation responses of target and non-target forest soil organisms to selected biocides\n\t\t\t\t\tSoil Biol. Biochem.\n\t\t\t\t\t26\n\t\t\t\t\t1\n\t\t\t\t\t41\n\t\t\t\t\t47\n\t\t\t\t\n\t\t\t'},{id:"B59",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tCortet\n\t\t\t\t\t\t\tJ.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tPoinsot-Balaguer\n\t\t\t\t\t\t\tN.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2000\n\t\t\t\t\tImpact of phytopharmaceutical products on soil microarthropods in an irrigated maize field: The use of the litter bag method.\n\t\t\t\t\tCan. J. Soil Sci.\n\t\t\t\t\t80\n\t\t\t\t\t2\n\t\t\t\t\t237\n\t\t\t\t\t249\n\t\t\t\t\n\t\t\t'},{id:"B60",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tCresswell\n\t\t\t\t\t\t\tJ.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2011\n\t\t\t\t\tA meta-analysis of experiments testing the effects of a neonicotinoid insecticide (imidacloprid) on honey bees\n\t\t\t\t\tEcotoxicology\n\t\t\t\t\t20\n\t\t\t\t\t1\n\t\t\t\t\t149\n\t\t\t\t\t157\n\t\t\t\t\n\t\t\t'},{id:"B61",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tCutler\n\t\t\t\t\t\t\tG. 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R.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tHejazi\n\t\t\t\t\t\t\tM. J.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tGanbalani\n\t\t\t\t\t\t\tG. N.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSaber\n\t\t\t\t\t\t\tM.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2008\n\t\t\t\t\tToxicity of some biorational and conventional insecticides to cotton bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae) and its ectoparasitoid, Habrobracon hebetor (Hymenoptera: Braconidae).\n\t\t\t\t\tJ. Entomol. Soc. Iran\n\t\t\t\t\t28\n\t\t\t\t\t1\n\t\t\t\t\t27\n\t\t\t\t\t37\n\t\t\t\t\n\t\t\t'},{id:"B65",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tDecarie\n\t\t\t\t\t\t\tR.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tDes\n\t\t\t\t\t\t\tGranges. J. L.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tLepine\n\t\t\t\t\t\t\tC.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMorneau\n\t\t\t\t\t\t\tF.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t1993\n\t\t\t\t\tImpact of insecticides on the American robin (Turdus migratorius) in a suburban environment.\n\t\t\t\t\tEnviron. Pollut.\n\t\t\t\t\t80\n\t\t\t\t\t231\n\t\t\t\t\t238\n\t\t\t\t\n\t\t\t'},{id:"B66",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tDecourtye\n\t\t\t\t\t\t\tA.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tDevillers\n\t\t\t\t\t\t\tJ.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tAupinel\n\t\t\t\t\t\t\tP.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tBrun\n\t\t\t\t\t\t\tF.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tBagnis\n\t\t\t\t\t\t\tC.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tFourrier\n\t\t\t\t\t\t\tJ.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tGauthier\n\t\t\t\t\t\t\tM.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2011\n\t\t\t\t\tHoneybee tracking with microchips: a new methodology to measure the effects of pesticides\n\t\t\t\t\tEcotoxicology\n\t\t\t\t\t20\n\t\t\t\t\t2\n\t\t\t\t\t429\n\t\t\t\t\t437\n\t\t\t\t\n\t\t\t'},{id:"B67",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tDecourtye\n\t\t\t\t\t\t\tA.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tDevillers\n\t\t\t\t\t\t\tJ.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tGenecque\n\t\t\t\t\t\t\tE.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMenach\n\t\t\t\t\t\t\tK. L.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tBudzinski\n\t\t\t\t\t\t\tH.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tCluzeau\n\t\t\t\t\t\t\tS.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tPham-Delègue\n\t\t\t\t\t\t\tM. H.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2005\n\t\t\t\t\tComparative sublethal toxicity of nine pesticides on olfactory learning performances of the honeybee Apis mellifera.\n\t\t\t\t\tArch. Environ. Contam. Toxicol.\n\t\t\t\t\t48\n\t\t\t\t\t2\n\t\t\t\t\t242\n\t\t\t\t\t250\n\t\t\t\t\n\t\t\t'},{id:"B68",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tDecourtye\n\t\t\t\t\t\t\tA.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tLacassie\n\t\t\t\t\t\t\tE.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tPham-Delègue\n\t\t\t\t\t\t\tM. H.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2003\n\t\t\t\t\tLearning performances of honeybees (Apis mellifera L) are differentially affected by imidacloprid according to the season.\n\t\t\t\t\tPest Manage. Sci.\n\t\t\t\t\t59\n\t\t\t\t\t3\n\t\t\t\t\t269\n\t\t\t\t\t278\n\t\t\t\t\n\t\t\t'},{id:"B69",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tDesneux\n\t\t\t\t\t\t\tN.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tDecourtye\n\t\t\t\t\t\t\tA.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tDelpuech\n\t\t\t\t\t\t\tJ.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\tM.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2007\n\t\t\t\t\tThe sublethal effects of pesticides on beneficial arthropods.\n\t\t\t\t\tAnnu. Rev. Entomol.\n\t\t\t\t\t52\n\t\t\t\t\t81\n\t\t\t\t\t106\n\t\t\t\t\n\t\t\t'},{id:"B70",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tDewar\n\t\t\t\t\t\t\tA. M.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tHaylock\n\t\t\t\t\t\t\tL. A.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tGarner\n\t\t\t\t\t\t\tB. H.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSands\n\t\t\t\t\t\t\tR. J. N.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tPilbrow\n\t\t\t\t\t\t\tJ.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2005\n\t\t\t\t\tNeonicotinoid seed treatments - the panacea for most pest problems in sugar beet.\n\t\t\t\t\tAspects Appl. Biol.\n\t\t\t\t\t76\n\t\t\t\t\t3\n\t\t\t\t\t12\n\t\t\t\t\n\t\t\t'},{id:"B71",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tDhadialla\n\t\t\t\t\t\t\tT. S.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tCarlson\n\t\t\t\t\t\t\tG. R.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tLe \n\t\t\t\t\t\t\tD. P.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t1998\n\t\t\t\t\tNew insecticides with ecdysteroidal and juvenile hormone activity.\n\t\t\t\t\tAnnu. Rev. Entomol.\n\t\t\t\t\t43\n\t\t\t\t\t1\n\t\t\t\t\t545\n\t\t\t\t\t569\n\t\t\t\t\n\t\t\t'},{id:"B72",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tDieter\n\t\t\t\t\t\t\tC. D.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tDuffy\n\t\t\t\t\t\t\tW. G.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tFlake\n\t\t\t\t\t\t\tL. 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Manage.\n\t\t\t\t\t59\n\t\t\t\t\t3\n\t\t\t\t\t498\n\t\t\t\t\t505\n\t\t\t\t\n\t\t\t'},{id:"B74",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tDittbrenner\n\t\t\t\t\t\t\tN.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tTriebskorn\n\t\t\t\t\t\t\tR.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMoser\n\t\t\t\t\t\t\tI.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tCapowiez\n\t\t\t\t\t\t\tY.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2010\n\t\t\t\t\tPhysiological and behavioural effects of imidacloprid on two ecologically relevant earthworm species (Lumbricus terrestris and Aporrectodea caliginosa)\n\t\t\t\t\tEcotoxicology\n\t\t\t\t\t19\n\t\t\t\t\t1567\n\t\t\t\t\t1573\n\t\t\t\t\n\t\t\t'},{id:"B75",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tDrescher\n\t\t\t\t\t\t\tW.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tGeusen-Pfister\n\t\t\t\t\t\t\tH.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t1991\n\t\t\t\t\tComparative testing of the oral toxicity of acephate, dimethoate and methomyl to honeybees, bumblebees and Syrphidae.\n\t\t\t\t\tActa Horticulturae\n\t\t\t\t\t288\n\t\t\t\t\t133\n\t\t\t\t\t138\n\t\t\t\t\n\t\t\t'},{id:"B76",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tEasterbrook\n\t\t\t\t\t\t\tM. A.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t1997\n\t\t\t\t\tA field assessment of the effects of insecticides on the beneficial fauna of strawberry\n\t\t\t\t\tCrop Protection\n\t\t\t\t\t16\n\t\t\t\t\t2\n\t\t\t\t\t147\n\t\t\t\t\t152\n\t\t\t\t\n\t\t\t'},{id:"B77",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tEcoli\n\t\t\t\t\t\t\tC. C.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMoraes\n\t\t\t\t\t\t\tJ. C.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tVilela\n\t\t\t\t\t\t\tM.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2010\n\t\t\t\t\tSuplementos alimentares e isca toxica no manejo do bicho-mineiro e de seus inimigos naturais.\n\t\t\t\t\tCoffee Sci.\n\t\t\t\t\t5\n\t\t\t\t\t2\n\t\t\t\t\t167\n\t\t\t\t\t172\n\t\t\t\t\n\t\t\t'},{id:"B78",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tEisenback\n\t\t\t\t\t\t\tB. M.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSalom\n\t\t\t\t\t\t\tS. M.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tKok\n\t\t\t\t\t\t\tL. T.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tLagalante\n\t\t\t\t\t\t\tA. F.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2010\n\t\t\t\t\tLethal and sublethal effects of imidacloprid on hemlock woolly adelgid (Hemiptera: Adelgidae) and two introduced predator species\n\t\t\t\t\tJ. Econ. Entomol.\n\t\t\t\t\t103\n\t\t\t\t\t4\n\t\t\t\t\t1222\n\t\t\t\t\t34\n\t\t\t\t\n\t\t\t'},{id:"B79",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tEisenhauer\n\t\t\t\t\t\t\tN.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tKlier\n\t\t\t\t\t\t\tM.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tPartsch\n\t\t\t\t\t\t\tS.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSabais\n\t\t\t\t\t\t\tA. C. W.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tScherber\n\t\t\t\t\t\t\tC.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tWeisser\n\t\t\t\t\t\t\tW. W.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tScheu\n\t\t\t\t\t\t\tS.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2009\n\t\t\t\t\tNo interactive effects of pesticides and plant diversity on soil microbial biomass and respiration\n\t\t\t\t\tAppl. Soil Ecol.\n\t\t\t\t\t42\n\t\t\t\t\t1\n\t\t\t\t\t31\n\t\t\t\t\t36\n\t\t\t\t\n\t\t\t'},{id:"B80",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tEl -Din\n\t\t\t\t\t\t\tH. A. S.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tGirgis\n\t\t\t\t\t\t\tN. R.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t1997\n\t\t\t\t\tSusceptibility of honey bee workers, Apis mellifera L. to nine different insecticides.\n\t\t\t\t\tAnn. Agric. Sci. Moshtohor\n\t\t\t\t\t35\n\t\t\t\t\t4\n\t\t\t\t\t2571\n\t\t\t\t\t2582\n\t\t\t\t\n\t\t\t'},{id:"B81",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tEl -Hassani\n\t\t\t\t\t\t\tA. K.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tDacher\n\t\t\t\t\t\t\tM.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tGary\n\t\t\t\t\t\t\tV.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tLambin\n\t\t\t\t\t\t\tM.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tGauthier\n\t\t\t\t\t\t\tM.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tArmengaud\n\t\t\t\t\t\t\tC.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2008\n\t\t\t\t\tEffects of sublethal doses of acetamiprid and thiamethoxam on the behavior of the honeybee (Apis mellifera)\n\t\t\t\t\tArch. Environ. Contam. Toxicol.\n\t\t\t\t\t54\n\t\t\t\t\t4\n\t\t\t\t\t653\n\t\t\t\t\t661\n\t\t\t\t\n\t\t\t'},{id:"B82",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tEl -Hassani\n\t\t\t\t\t\t\tA. K.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tDacher\n\t\t\t\t\t\t\tM.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tGauthier\n\t\t\t\t\t\t\tM.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tArmengaud\n\t\t\t\t\t\t\tC.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2005\n\t\t\t\t\tEffects of sublethal doses of fipronil on the behavior of the honeybee (Apis mellifera)\n\t\t\t\t\tPharmacol. Biochem. Behavior\n\t\t\t\t\t82\n\t\t\t\t\t1\n\t\t\t\t\t30\n\t\t\t\t\t39\n\t\t\t\t\n\t\t\t'},{id:"B83",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tElbert\n\t\t\t\t\t\t\tA.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tHaas\n\t\t\t\t\t\t\tM.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSpringer\n\t\t\t\t\t\t\tB.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tThielert\n\t\t\t\t\t\t\tW.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tNauen\n\t\t\t\t\t\t\tR.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2008\n\t\t\t\t\tApplied aspects of neonicotinoid uses in crop protection\n\t\t\t\t\tPest Manage. Sci.\n\t\t\t\t\t64\n\t\t\t\t\t11\n\t\t\t\t\t1099\n\t\t\t\t\t1105\n\t\t\t\t\n\t\t\t'},{id:"B84",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tElliott\n\t\t\t\t\t\t\tJ. E.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tWilson\n\t\t\t\t\t\t\tL. K.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tLangelier\n\t\t\t\t\t\t\tK. M.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMineau\n\t\t\t\t\t\t\tP.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSinclair\n\t\t\t\t\t\t\tP. H.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t1997\n\t\t\t\t\tSecondary poisoning of birds of prey by the organophosphorus insecticide, phorate\n\t\t\t\t\tEcotoxicology\n\t\t\t\t\t6\n\t\t\t\t\t4\n\t\t\t\t\t219\n\t\t\t\t\t231\n\t\t\t\t\n\t\t\t'},{id:"B85",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tEndlweber\n\t\t\t\t\t\t\tK.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSchädler\n\t\t\t\t\t\t\tM.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tScheu\n\t\t\t\t\t\t\tS.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2005\n\t\t\t\t\tEffects of foliar and soil insecticide applications on the collembolan community of an early set-aside arable field\n\t\t\t\t\tAppl. Soil Ecol.\n\t\t\t\t\t31\n\t\t\t\t\t1-2\n\t\t\t\t\t136\n\t\t\t\t\t146\n\t\t\t\t\n\t\t\t'},{id:"B86",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tFabian\n\t\t\t\t\t\t\tM.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tPetersen\n\t\t\t\t\t\t\tH.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t1994\n\t\t\t\t\tShort-term effects of the insecticide dimethoate on activity and spatial distribution of a soil inhabiting collembolan Folsomia fimetaria Linne (Collembola:Isotomidae).\n\t\t\t\t\tPedobiologia\n\t\t\t\t\t38\n\t\t\t\t\t4\n\t\t\t\t\t289\n\t\t\t\t\t302\n\t\t\t\t\n\t\t\t'},{id:"B87",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tFalcone\n\t\t\t\t\t\t\tJ. F.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tDe Wald\n\t\t\t\t\t\t\tL. E.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2010\n\t\t\t\t\tComparisons of arthropod and avian assemblages in insecticide-treated and untreated eastern hemlock (Tsuga canadensis L. Carr) stands in Great Smoky Mountains National Park, USA.\n\t\t\t\t\tForest Ecol. Manage.\n\t\t\t\t\t260\n\t\t\t\t\t5\n\t\t\t\t\t856\n\t\t\t\t\t863\n\t\t\t\t\n\t\t\t'},{id:"B88",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tFarinos\n\t\t\t\t\t\t\tG. P.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tde la Poza\n\t\t\t\t\t\t\tM.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tHernandez-Crespo\n\t\t\t\t\t\t\tP.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tOrtego\n\t\t\t\t\t\t\tF.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tCastanera\n\t\t\t\t\t\t\tP.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2008\n\t\t\t\t\tDiversity and seasonal phenology of aboveground arthropods in conventional and transgenic maize crops in Central Spain\n\t\t\t\t\tBiol. Control\n\t\t\t\t\t44\n\t\t\t\t\t3\n\t\t\t\t\t362\n\t\t\t\t\t371\n\t\t\t\t\n\t\t\t'},{id:"B89",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tFaucon\n\t\t\t\t\t\t\tJ. P.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tAurières\n\t\t\t\t\t\t\tC.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tDrajnudel\n\t\t\t\t\t\t\tP.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMathieu\n\t\t\t\t\t\t\tL.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tRibière\n\t\t\t\t\t\t\tM.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMartel\n\t\t\t\t\t\t\tA. C.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tZeggane\n\t\t\t\t\t\t\tS.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tChauzat\n\t\t\t\t\t\t\tM. P.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tAubert\n\t\t\t\t\t\t\tM. F. A.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2005\n\t\t\t\t\tExperimental study on the toxicity of imidacloprid given in syrup to honey bee (Apis mellifera) colonies.\n\t\t\t\t\tPest Manage. Sci.\n\t\t\t\t\t61\n\t\t\t\t\t2\n\t\t\t\t\t111\n\t\t\t\t\t125\n\t\t\t\t\n\t\t\t'},{id:"B90",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tFernandes\n\t\t\t\t\t\t\tM.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tE.d\n\t\t\t\t\t\t\tS.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tFernandes\n\t\t\t\t\t\t\tF. L.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tPicanco\n\t\t\t\t\t\t\tM. C.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tQueiroz\n\t\t\t\t\t\t\tR. B.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSilva\n\t\t\t\t\t\t\tR. S.d\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tHuertas\n\t\t\t\t\t\t\tA. A. G.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2008\n\t\t\t\t\tPhysiological selectivity of insecticides to Apis mellifera (Hymenoptera: Apidae) and Protonectarina sylveirae (Hymenoptera: Vespidae) in citrus\n\t\t\t\t\tSociobiology\n\t\t\t\t\t51\n\t\t\t\t\t3\n\t\t\t\t\t765\n\t\t\t\t\t774\n\t\t\t\t\n\t\t\t'},{id:"B91",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tFleming\n\t\t\t\t\t\t\tW.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tBradbury\n\t\t\t\t\t\t\tS.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t1981\n\t\t\t\t\tRecovery of cholinesterase activity in mallard ducklings administered organophosphorus pesticides.\n\t\t\t\t\tJ. Toxicol. Environ. Health B\n\t\t\t\t\t8\n\t\t\t\t\t5-6\n\t\t\t\t\t885\n\t\t\t\t\t97\n\t\t\t\t\n\t\t\t'},{id:"B92",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tFlickinger\n\t\t\t\t\t\t\tE. L.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tWhite\n\t\t\t\t\t\t\tD. H.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMitchell\n\t\t\t\t\t\t\tC. A.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tLamont\n\t\t\t\t\t\t\tT. G.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t1984\n\t\t\t\t\tMonocrotophos and dicrotophos residues in birds as a result of misuse of organophosphates in Matagorda County, Texas.\n\t\t\t\t\tJ.A.O.A.C.\n\t\t\t\t\t67\n\t\t\t\t\t827\n\t\t\t\t\t828\n\t\t\t\t\n\t\t\t'},{id:"B93",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tFluetsch\n\t\t\t\t\t\t\tK. M.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSparling\n\t\t\t\t\t\t\tD. W.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t1994\n\t\t\t\t\tAvian nesting success and diversity in conventionally and organically managed apple orchards\n\t\t\t\t\tEnviron. Toxicol. Chem.\n\t\t\t\t\t13\n\t\t\t\t\t10\n\t\t\t\t\t1651\n\t\t\t\t\t1659\n\t\t\t\t\n\t\t\t'},{id:"B94",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tFowle\n\t\t\t\t\t\t\tC. D.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t1966The effects of phosphamidon on birds in New Brunswick forests\n\t\t\t\t\tJ. Appl. Ecol.\n\t\t\t\t\t3\n\t\t\t\t\t169\n\t\t\t\t\t170\n\t\t\t\t\n\t\t\t'},{id:"B95",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tFrampton\n\t\t\t\t\t\t\tG. K.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tBrink\n\t\t\t\t\t\t\tP.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tJ.v.d\n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2007\n\t\t\t\t\tCollembola and macroarthropod community responses to carbamate, organophosphate and synthetic pyrethroid insecticides: direct and indirect effects\n\t\t\t\t\tEnviron. Pollut.\n\t\t\t\t\t147\n\t\t\t\t\t1\n\t\t\t\t\t14\n\t\t\t\t\t25\n\t\t\t\t\n\t\t\t'},{id:"B96",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tFranklin\n\t\t\t\t\t\t\tM. T.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tWinston\n\t\t\t\t\t\t\tM. L.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMorandin\n\t\t\t\t\t\t\tL. A.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2004\n\t\t\t\t\tEffects of clothianidin on Bombus impatiens (Hymenoptera: Apidae) colony health and foraging ability\n\t\t\t\t\tJ. Econ. Entomol.\n\t\t\t\t\t97\n\t\t\t\t\t2\n\t\t\t\t\t369\n\t\t\t\t\t373\n\t\t\t\t\n\t\t\t'},{id:"B97",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tFreuler\n\t\t\t\t\t\t\tJ.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tBlandenier\n\t\t\t\t\t\t\tG.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMeyer\n\t\t\t\t\t\t\tH.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tPignon\n\t\t\t\t\t\t\tP.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2001\n\t\t\t\t\tEpigeal fauna in a vegetable agroecosystem.\n\t\t\t\t\tMitteilungen der Schweizerischen Entomologischen Gesellschaft\n\t\t\t\t\t74\n\t\t\t\t\t1-2\n\t\t\t\t\t17\n\t\t\t\t\t42\n\t\t\t\t\n\t\t\t'},{id:"B98",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tFurlong\n\t\t\t\t\t\t\tM. J.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tVerkerk\n\t\t\t\t\t\t\tR. H. 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Saf.\n\t\t\t\t\t80\n\t\t\t\t\t355\n\t\t\t\t\t362\n\t\t\t\t\n\t\t\t'},{id:"B119",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tHayasaka\n\t\t\t\t\t\t\tD.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tKorenaga\n\t\t\t\t\t\t\tT.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSuzuki\n\t\t\t\t\t\t\tK.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSánchez-Bayo\n\t\t\t\t\t\t\tF.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tGoka\n\t\t\t\t\t\t\tK.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2012c\n\t\t\t\t\tDifferences in susceptibility of five cladoceran species to two systemic insecticides, imidacloprid and fipronil\n\t\t\t\t\tEcotoxicology\n\t\t\t\t\t21\n\t\t\t\t\t2\n\t\t\t\t\t421\n\t\t\t\t\t427\n\t\t\t\t\n\t\t\t'},{id:"B120",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tHe\n\t\t\t\t\t\t\tY.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tZhao\n\t\t\t\t\t\t\tJ.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tZheng\n\t\t\t\t\t\t\tY.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tDesneux\n\t\t\t\t\t\t\tN.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tWu\n\t\t\t\t\t\t\tK.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2012\n\t\t\t\t\tLethal effect of imidacloprid on the coccinellid predator Serangium japonicum and sublethal effects on predator voracity and on functional response to the whitefly Bemisia tabaci\n\t\t\t\t\tEcotoxicology\n\t\t\t\t\t1\n\t\t\t\t\t10\n\t\t\t\t\n\t\t\t'},{id:"B121",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tHeath\n\t\t\t\t\t\t\tA. 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Soil Ecol.\n\t\t\t\t\t17\n\t\t\t\t\t3\n\t\t\t\t\t253\n\t\t\t\t\t266\n\t\t\t\t\n\t\t\t'},{id:"B145",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tKitulagodage\n\t\t\t\t\t\t\tM.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tButtemer\n\t\t\t\t\t\t\tW.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tAstheimer\n\t\t\t\t\t\t\tL.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2011\n\t\t\t\t\tAdverse effects of fipronil on avian reproduction and development: maternal transfer of fipronil to eggs in zebra finch Taeniopygia guttata and in ovo exposure in chickens Gallus domesticus.\n\t\t\t\t\tEcotoxicology\n\t\t\t\t\t20\n\t\t\t\t\t4\n\t\t\t\t\t653\n\t\t\t\t\t660\n\t\t\t\t\n\t\t\t'},{id:"B146",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tKjaer\n\t\t\t\t\t\t\tC.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tElmegaard\n\t\t\t\t\t\t\tN.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tAxelsen\n\t\t\t\t\t\t\tJ. A.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tAndersen\n\t\t\t\t\t\t\tP. 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H.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t1997\n\t\t\t\t\tMesostigmata (Gamasina, Uropodina), efficient predators in agroecosystems.\n\t\t\t\t\tAgric. Ecosyst. Environ.\n\t\t\t\t\t62\n\t\t\t\t\t2-3\n\t\t\t\t\t105\n\t\t\t\t\t117\n\t\t\t\t\n\t\t\t'},{id:"B149",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tKoppenhofer\n\t\t\t\t\t\t\tA. M.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tCowles\n\t\t\t\t\t\t\tR. S.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tCowles\n\t\t\t\t\t\t\tE. A.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tFuzy\n\t\t\t\t\t\t\tE. M.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tKaya\n\t\t\t\t\t\t\tH. K.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2003\n\t\t\t\t\tEffect of neonicotinoid synergists on entomopathogenic nematode fitness\n\t\t\t\t\tEntomol. exp. appl.\n\t\t\t\t\t106\n\t\t\t\t\t1\n\t\t\t\t\t7\n\t\t\t\t\t18\n\t\t\t\t\n\t\t\t'},{id:"B150",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tKreutzweiser\n\t\t\t\t\t\t\tD. 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Sci.\n\t\t\t\t\t67\n\t\t\t\t\t10\n\t\t\t\t\t1245\n\t\t\t\t\t1252\n\t\t\t\t\n\t\t\t'},{id:"B173",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tLoureiro\n\t\t\t\t\t\t\tS.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSvendsen\n\t\t\t\t\t\t\tC.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tFerreira\n\t\t\t\t\t\t\tA. L. G.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tPinheiro\n\t\t\t\t\t\t\tC.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tRibeiro\n\t\t\t\t\t\t\tF.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSoares\n\t\t\t\t\t\t\tA. M. V. M.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2010\n\t\t\t\t\tToxicity of three binary mixtures to Daphnia magna: Comparing chemical modes of action and deviations from conceptual models\n\t\t\t\t\tEnviron. Toxicol. Chem.\n\t\t\t\t\t29\n\t\t\t\t\t8\n\t\t\t\t\t1716\n\t\t\t\t\t1726\n\t\t\t\t\n\t\t\t'},{id:"B174",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tLu\n\t\t\t\t\t\t\tC.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tWarchol\n\t\t\t\t\t\t\tK. M.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tCallahan\n\t\t\t\t\t\t\tR. A.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2012\n\t\t\t\t\tIn situ replication of honey bee colony collapse disorder\n\t\t\t\t\tBull. Insectology\n\t\t\t\t\t65\n\t\t\t\t\t1\n\t\t\t\t\t99\n\t\t\t\t\t106\n\t\t\t\t\n\t\t\t'},{id:"B175",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tLue\n\t\t\t\t\t\t\tL. P.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tLewis\n\t\t\t\t\t\t\tC. C.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMelchor\n\t\t\t\t\t\t\tV. E.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t1984\n\t\t\t\t\tThe effect of aldicarb on nematode population and its persistence in carrots, soil and hydroponic solution.\n\t\t\t\t\tJ. Environ. Sci. Health B\n\t\t\t\t\t19\n\t\t\t\t\t3\n\t\t\t\t\t343\n\t\t\t\t\t354\n\t\t\t\t\n\t\t\t'},{id:"B176",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMaccagnani\n\t\t\t\t\t\t\tB.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tFerrari\n\t\t\t\t\t\t\tR.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tZucchi\n\t\t\t\t\t\t\tL.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tBariselli\n\t\t\t\t\t\t\tM.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2008\n\t\t\t\t\tNei medicai dell’emilia-romagna: difendersi dalle cavallette, ma tutelare le apiInformatore Agrario\n\t\t\t\t\t64\n\t\t\t\t\t25\n\t\t\t\t\t53\n\t\t\t\t\t56\n\t\t\t\t\n\t\t\t'},{id:"B177",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMagagula\n\t\t\t\t\t\t\tC. N.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSamways\n\t\t\t\t\t\t\tM. J.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2000\n\t\t\t\t\tEffects of insect growth regulators on Chilocorus nigritus (Fabricius) (Coleoptera: Coccinellidae), a non-target natural enemy of citrus red scale, Aonidiella aurantii (Maskell) (Homoptera: Diaspididae), in southern Africa: evidence from laboratory and field trials.\n\t\t\t\t\tAfrican Entomol.\n\t\t\t\t\t8\n\t\t\t\t\t1\n\t\t\t\t\t47\n\t\t\t\t\t56\n\t\t\t\t\n\t\t\t'},{id:"B178",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMalinowski\n\t\t\t\t\t\t\tH.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2006\n\t\t\t\t\tBioroznorodnosc a ochrona lasu przed szkodliwymi owadami.\n\t\t\t\t\tProgress in Plant Protection\n\t\t\t\t\t46\n\t\t\t\t\t1\n\t\t\t\t\t319\n\t\t\t\t\t325\n\t\t\t\t\n\t\t\t'},{id:"B179",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMarletto\n\t\t\t\t\t\t\tF.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tPatetta\n\t\t\t\t\t\t\tA.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tManino\n\t\t\t\t\t\t\tA.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2003\n\t\t\t\t\tLaboratory assessment of pesticide toxicity to bumble bees.\n\t\t\t\t\tBull. Insectology\n\t\t\t\t\t56\n\t\t\t\t\t1\n\t\t\t\t\t155\n\t\t\t\t\t158\n\t\t\t\t\n\t\t\t'},{id:"B180",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMartikainen\n\t\t\t\t\t\t\tE.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tHaimi\n\t\t\t\t\t\t\tJ.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tAhtiainen\n\t\t\t\t\t\t\tJ.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t1998\n\t\t\t\t\tEffects of dimethoate and benomyl on soil organisms and soil processes- a microcosm study.\n\t\t\t\t\tAppl. Soil Ecol.\n\t\t\t\t\t9\n\t\t\t\t\t1-3\n\t\t\t\t\t381\n\t\t\t\t\t387\n\t\t\t\t\n\t\t\t'},{id:"B181",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMartins\n\t\t\t\t\t\t\tG. L. M.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tToscano\n\t\t\t\t\t\t\tL. C.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tTomquelski\n\t\t\t\t\t\t\tG. V.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMaruyama\n\t\t\t\t\t\t\tW. 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P.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tShanks\n\t\t\t\t\t\t\tC. H.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t1994\n\t\t\t\t\tDifferences between susceptibility of four pollinator species (Hymenoptera: Apoidea) to field weathered insecticide residues.\n\t\t\t\t\tMelanderia\n\t\t\t\t\t50\n\t\t\t\t\t24\n\t\t\t\t\t27\n\t\t\t\t\n\t\t\t'},{id:"B186",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMedrzycki\n\t\t\t\t\t\t\tP.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMontanari\n\t\t\t\t\t\t\tR.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tBortolotti\n\t\t\t\t\t\t\tL.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSabatini\n\t\t\t\t\t\t\tA. G.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMaini\n\t\t\t\t\t\t\tS.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tPorrini\n\t\t\t\t\t\t\tC.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2003\n\t\t\t\t\tEffects of imidacloprid administered in sublethal doses on honey bee behaviour. Laboratory tests.\n\t\t\t\t\tBull. Insectology\n\t\t\t\t\t56\n\t\t\t\t\t1\n\t\t\t\t\t59\n\t\t\t\t\t62\n\t\t\t\t\n\t\t\t'},{id:"B187",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMeher\n\t\t\t\t\t\t\tH. C.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tGajbhiye\n\t\t\t\t\t\t\tV. T.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSingh\n\t\t\t\t\t\t\tG.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tKamra\n\t\t\t\t\t\t\tA.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tChawla\n\t\t\t\t\t\t\tG.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2010\n\t\t\t\t\tPersistence and nematicidal efficacy of carbosulfan, cadusafos, phorate, and triazophos in soil and uptake by chickpea and tomato crops under tropical conditions.\n\t\t\t\t\tJ. Agric. Food Chem.\n\t\t\t\t\t58\n\t\t\t\t\t3\n\t\t\t\t\t1815\n\t\t\t\t\t1822\n\t\t\t\t\n\t\t\t'},{id:"B188",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMesléard\n\t\t\t\t\t\t\tF.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tGarnero\n\t\t\t\t\t\t\tS.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tBeck\n\t\t\t\t\t\t\tN.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tRosecchi\n\t\t\t\t\t\t\tE.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2005\n\t\t\t\t\tUselessness and indirect negative effects of an insecticide on rice field invertebrates.\n\t\t\t\t\tComptes Rendus Biologies\n\t\t\t\t\t328\n\t\t\t\t\t10-11\n\t\t\t\t\t955\n\t\t\t\t\t62\n\t\t\t\t\n\t\t\t'},{id:"B189",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMineau\n\t\t\t\t\t\t\tP.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t1988\n\t\t\t\t\tAvian mortality in agroecosystems. I. 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Risk Assess.\n\t\t\t\t\t17\n\t\t\t\t\t4\n\t\t\t\t\t981\n\t\t\t\t\t995\n\t\t\t\t\n\t\t\t'},{id:"B192",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMoens\n\t\t\t\t\t\t\tJ.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tTirry\n\t\t\t\t\t\t\tL.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tClercq\n\t\t\t\t\t\t\tP.d.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2012\n\t\t\t\t\tSusceptibility of cocooned pupae and adults of the parasitoid Microplitis mediator to selected insecticides\n\t\t\t\t\tPhytoparasitica\n\t\t\t\t\t40\n\t\t\t\t\t1\n\t\t\t\t\t5\n\t\t\t\t\t9\n\t\t\t\t\n\t\t\t'},{id:"B193",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMommaerts\n\t\t\t\t\t\t\tV.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tReynders\n\t\t\t\t\t\t\tS.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tBoulet\n\t\t\t\t\t\t\tJ.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tBesard\n\t\t\t\t\t\t\tL.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSterk\n\t\t\t\t\t\t\tG.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSmagghe\n\t\t\t\t\t\t\tG.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2010\n\t\t\t\t\tRisk assessment for side-effects of neonicotinoids against bumblebees with and without impairing foraging behavior\n\t\t\t\t\tEcotoxicology\n\t\t\t\t\t19\n\t\t\t\t\t1\n\t\t\t\t\t207\n\t\t\t\t\t215\n\t\t\t\t\n\t\t\t'},{id:"B194",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMorandin\n\t\t\t\t\t\t\tL. 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Chem.\n\t\t\t\t\t20\n\t\t\t\t\t10\n\t\t\t\t\t2243\n\t\t\t\t\t2254\n\t\t\t\t\n\t\t\t'},{id:"B196",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMoser\n\t\t\t\t\t\t\tS. E.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tObrycki\n\t\t\t\t\t\t\tJ. J.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2009\n\t\t\t\t\tNon-target effects of neonicotinoid seed treatments; mortality of coccinellid larvae related to zoophytophagy\n\t\t\t\t\tBiol. Control\n\t\t\t\t\t51\n\t\t\t\t\t3\n\t\t\t\t\t487\n\t\t\t\t\t492\n\t\t\t\t\n\t\t\t'},{id:"B197",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMoser\n\t\t\t\t\t\t\tV. C.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMc Daniel\n\t\t\t\t\t\t\tK. L.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tPhillips\n\t\t\t\t\t\t\tP. M.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tLowit\n\t\t\t\t\t\t\tA. B.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2010\n\t\t\t\t\tTime-course, dose-response, and age comparative sensitivity of N-methyl carbamates in rats.\n\t\t\t\t\tToxicol. 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E.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t1996\n\t\t\t\t\tEffects of two cholinesterase-inhibiting pesticides on freshwater mussels\n\t\t\t\t\tEnviron. Toxicol. Chem.\n\t\t\t\t\t15\n\t\t\t\t\t2\n\t\t\t\t\t131\n\t\t\t\t\t137\n\t\t\t\t\n\t\t\t'},{id:"B200",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMullin\n\t\t\t\t\t\t\tC. A.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tFrazier\n\t\t\t\t\t\t\tM.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tFrazier\n\t\t\t\t\t\t\tJ. L.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tAshcraft\n\t\t\t\t\t\t\tS.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSimonds\n\t\t\t\t\t\t\tR.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tD.v\n\t\t\t\t\t\t\tE.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tPettis\n\t\t\t\t\t\t\tJ. 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Sci.\n\t\t\t\t\t55\n\t\t\t\t\t11\n\t\t\t\t\t1077\n\t\t\t\t\t1082\n\t\t\t\t\n\t\t\t'},{id:"B212",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tPerfecto\n\t\t\t\t\t\t\tI.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t1990\n\t\t\t\t\tIndirect and direct effects in a tropical agroecosystem: the maize-pest-ant system in Nicaragua.\n\t\t\t\t\t Ecology\n\t\t\t\t\t71\n\t\t\t\t\t6\n\t\t\t\t\t2125\n\t\t\t\t\t2134\n\t\t\t\t\n\t\t\t'},{id:"B213",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tPettis\n\t\t\t\t\t\t\tJ.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tvan Engelsdorp\n\t\t\t\t\t\t\tD.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tJohnson\n\t\t\t\t\t\t\tJ.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tDively\n\t\t\t\t\t\t\tG.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2012\n\t\t\t\t\tPesticide exposure in honey bees results in increased levels of the gut pathogen NosemaNaturwissenschaften99\n\t\t\t\t\t2\n\t\t\t\t\t153\n\t\t\t\t\t158\n\t\t\t\t\n\t\t\t'},{id:"B214",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tPeveling\n\t\t\t\t\t\t\tR.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMc William\n\t\t\t\t\t\t\tA. 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Chem.\n\t\t\t\t\t30\n\t\t\t\t\t8\n\t\t\t\t\t1926\n\t\t\t\t\t1934\n\t\t\t\t\n\t\t\t'},{id:"B224",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tRainwater\n\t\t\t\t\t\t\tT. R.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tLeopold\n\t\t\t\t\t\t\tV. A.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tHooper\n\t\t\t\t\t\t\tM. J.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tKendall\n\t\t\t\t\t\t\tR. J.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t1995\n\t\t\t\t\tAvian exposure to organophosphorous and carbamate pesticides on a coastal South Carolina golf course.\n\t\t\t\t\tEnviron. Toxicol. Chem.\n\t\t\t\t\t14\n\t\t\t\t\t12\n\t\t\t\t\t2155\n\t\t\t\t\t2161\n\t\t\t\t\n\t\t\t'},{id:"B225",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tRam\n\t\t\t\t\t\t\tS.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tGupta\n\t\t\t\t\t\t\tG. P.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t1994\n\t\t\t\t\tBioefficacy of systemic insecticides against target pest jassid (Amrasca devastans) and their impact on non-target soil microarthropods in cotton (Gossypium spp.).\n\t\t\t\t\tIndian J. Entomol.\n\t\t\t\t\t56\n\t\t\t\t\t4\n\t\t\t\t\t313\n\t\t\t\t\t321\n\t\t\t\t\n\t\t\t'},{id:"B226",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tRedoan\n\t\t\t\t\t\t\tA. C.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tCarvalho\n\t\t\t\t\t\t\tG. A.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tCruz\n\t\t\t\t\t\t\tI.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tFigueiredo\n\t\t\t\t\t\t\tM.d. L. C.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSilva\n\t\t\t\t\t\t\tR. B.d\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2010\n\t\t\t\t\tEfeito de inseticidas usados na cultura do milho (Zea mays L.) sobre ninfas e adultos de Doru luteipes (Scudder) (Dermaptera: Forficulidae) em semicampo.\n\t\t\t\t\tRevista Brasileira de Milho e Sorgo\n\t\t\t\t\t9\n\t\t\t\t\t3\n\t\t\t\t\t223\n\t\t\t\t\t235\n\t\t\t\t\n\t\t\t'},{id:"B227",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tRodrigues\n\t\t\t\t\t\t\tE.d. L.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tFanta\n\t\t\t\t\t\t\tE.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t1998\n\t\t\t\t\tLiver histopathology of the fish Brachydanio rerio Hamilton-Buchman after acute exposure to sublethal levels of the organophosphate dimethoate 500\n\t\t\t\t\tRevista Brasileira de Zoologia\n\t\t\t\t\t15\n\t\t\t\t\t2\n\t\t\t\t\t441\n\t\t\t\t\t450\n\t\t\t\t\n\t\t\t'},{id:"B228",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tRortais\n\t\t\t\t\t\t\tA.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tArnold\n\t\t\t\t\t\t\tG.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tHalm\n\t\t\t\t\t\t\tM. 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Toxicol.\n\t\t\t\t\t13\n\t\t\t\t\t367\n\t\t\t\t\t372\n\t\t\t\t\n\t\t\t'},{id:"B230",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tRust\n\t\t\t\t\t\t\tM. K.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSaran\n\t\t\t\t\t\t\tR. K.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2008\n\t\t\t\t\tToxicity, repellency, and effects of acetamiprid on western subterranean termite (Isoptera: Rhinotermitidae).\n\t\t\t\t\tJ. Econ. Entomol.\n\t\t\t\t\t101\n\t\t\t\t\t4\n\t\t\t\t\t1360\n\t\t\t\t\t1366\n\t\t\t\t\n\t\t\t'},{id:"B231",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSaber\n\t\t\t\t\t\t\tM.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2011\n\t\t\t\t\tAcute and population level toxicity of imidacloprid and fenpyroximate on an important egg parasitoid, Trichogramma cacoeciae (Hymenoptera: Trichogrammatidae)\n\t\t\t\t\tEcotoxicology\n\t\t\t\t\t20\n\t\t\t\t\t6\n\t\t\t\t\t1476\n\t\t\t\t\t1484\n\t\t\t\t\n\t\t\t'},{id:"B232",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSan\n\t\t\t\t\t\t\tMiguel. A.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tRaveton\n\t\t\t\t\t\t\tM.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tLemperiere\n\t\t\t\t\t\t\tG.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tRavanel\n\t\t\t\t\t\t\tP.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2008\n\t\t\t\t\tPhenylpyrazoles impact on Folsomia candida (Collembola). 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Health B\n\t\t\t\t\t45\n\t\t\t\t\t3\n\t\t\t\t\t214\n\t\t\t\t\t221\n\t\t\t\t\n\t\t\t'},{id:"B234",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSánchez-Bayo\n\t\t\t\t\t\t\tF.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2009\n\t\t\t\t\tFrom simple toxicological models to prediction of toxic effects in time\n\t\t\t\t\tEcotoxicology\n\t\t\t\t\t18\n\t\t\t\t\t3\n\t\t\t\t\t343\n\t\t\t\t\t354\n\t\t\t\t\n\t\t\t'},{id:"B235",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSánchez-Bayo\n\t\t\t\t\t\t\tF.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2012\n\t\t\t\t\tInsecticides mode of action in relation to their toxicity to non-target organisms.\n\t\t\t\t\tJ. Environ. Anal. 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Chem.\n\t\t\t\t\t25\n\t\t\t\t\t6\n\t\t\t\t\t1677\n\t\t\t\t\t1687\n\t\t\t\t\n\t\t\t'},{id:"B238",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSánchez-Bayo\n\t\t\t\t\t\t\tF.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tYamashita\n\t\t\t\t\t\t\tH.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tOsaka\n\t\t\t\t\t\t\tR.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tYoneda\n\t\t\t\t\t\t\tM.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tGoka\n\t\t\t\t\t\t\tK.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2007\n\t\t\t\t\tEcological effects of imidacloprid on arthropod communities in and around a vegetable crop.\n\t\t\t\t\tJ. Environ. Sci. Health B\n\t\t\t\t\t42\n\t\t\t\t\t3\n\t\t\t\t\t279\n\t\t\t\t\t286\n\t\t\t\t\n\t\t\t'},{id:"B239",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSchaefer\n\t\t\t\t\t\t\tC. H.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMiura\n\t\t\t\t\t\t\tT.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tDupras\n\t\t\t\t\t\t\tE. F. Jr\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tWilder\n\t\t\t\t\t\t\tW. H.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMulligan\n\t\t\t\t\t\t\tF. S. III\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t1988\n\t\t\t\t\tEfficacy of CME 134 against mosquitoes (Diptera: Culicidae): effects on nontarget organisms and evaluation of potential chemical persistence.\n\t\t\t\t\tJ. Econ. Entomol.\n\t\t\t\t\t81\n\t\t\t\t\t4\n\t\t\t\t\t1128\n\t\t\t\t\t1132\n\t\t\t\t\n\t\t\t'},{id:"B240",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSchmuck\n\t\t\t\t\t\t\tR.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2004\n\t\t\t\t\tEffects of a chronic dietary exposure of the honeybee Apis mellifera (Hymenoptera: Apidae) to imidacloprid.\n\t\t\t\t\tArch. Environ. Contam. 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W.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2003\n\t\t\t\t\tField relevance of a synergistic effect observed in the laboratory between an EBI fungicide and a chloronicotinyl insecticide in the honeybee (Apis mellifera L, Hymenoptera).\n\t\t\t\t\tPest Manage. Sci.\n\t\t\t\t\t59\n\t\t\t\t\t3\n\t\t\t\t\t279\n\t\t\t\t\t286\n\t\t\t\t\n\t\t\t'},{id:"B243",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSchneider\n\t\t\t\t\t\t\tF.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t1966\n\t\t\t\t\tSome pesticide-wildlife problems in Switzerland\n\t\t\t\t\tJ. Appl. Ecol.\n\t\t\t\t\t3\n\t\t\t\t\t15\n\t\t\t\t\t20\n\t\t\t\t\n\t\t\t'},{id:"B244",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSechser\n\t\t\t\t\t\t\tB.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tFreuler\n\t\t\t\t\t\t\tJ.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2003\n\t\t\t\t\tThe impact of thiamethoxam on bumble bee broods (Bombus terrestris L.) following drip application in covered tomato crops\n\t\t\t\t\tAnzeiger fur Schadlingskunde\n\t\t\t\t\t76\n\t\t\t\t\t3\n\t\t\t\t\t74\n\t\t\t\t\t77\n\t\t\t\t\n\t\t\t'},{id:"B245",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSétamou\n\t\t\t\t\t\t\tM.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tRodriguez\n\t\t\t\t\t\t\tD.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSaldana\n\t\t\t\t\t\t\tR.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSchwarzlose\n\t\t\t\t\t\t\tG.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tPalrang\n\t\t\t\t\t\t\tD.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tNelson\n\t\t\t\t\t\t\tS. D.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2010\n\t\t\t\t\tEfficacy and uptake of soil-applied imidacloprid in the control of Asian citrus psyllid and a citrus leafminer, two foliar-feeding citrus pests\n\t\t\t\t\tJ. Econ. Entomol.\n\t\t\t\t\t103\n\t\t\t\t\t5\n\t\t\t\t\t1711\n\t\t\t\t\t9\n\t\t\t\t\n\t\t\t'},{id:"B246",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSharma\n\t\t\t\t\t\t\tD. R.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2010\n\t\t\t\t\tBioefficacy of insecticides against peach leaf curl aphid, Brachycaudus helichrysi (Kaltenbach) in Punjab\n\t\t\t\t\tIndian J. 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Sci.67\n\t\t\t\t\t12\n\t\t\t\t\t1528\n\t\t\t\t\t1533\n\t\t\t\t\n\t\t\t'},{id:"B248",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tShimoda\n\t\t\t\t\t\t\tT.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tYara\n\t\t\t\t\t\t\tK.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tKawazu\n\t\t\t\t\t\t\tK.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2011\n\t\t\t\t\tThe effects of eight insecticides on the foraging behavior of the parasitoid wasp Cotesia vestalisJ. Plant Interact.6\n\t\t\t\t\t2/3\n\t\t\t\t\t189\n\t\t\t\t\t190\n\t\t\t\t\n\t\t\t'},{id:"B249",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSimpson\n\t\t\t\t\t\t\tI. C.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tRoger\n\t\t\t\t\t\t\tP. A.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tOficial\n\t\t\t\t\t\t\tR.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tGrant\n\t\t\t\t\t\t\tI. 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Pest Sci.\n\t\t\t\t\t84\n\t\t\t\t\t1\n\t\t\t\t\t25\n\t\t\t\t\t31\n\t\t\t\t\n\t\t\t'},{id:"B259",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tStark\n\t\t\t\t\t\t\tJ.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tVargas\n\t\t\t\t\t\t\tR.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2005\n\t\t\t\t\tToxicity and hazard assessment of fipronil to Daphnia pulex Ecotoxicol.Environ. Saf. 62\n\t\t\t\t\t1\n\t\t\t\t\t11\n\t\t\t\t\t16\n\t\t\t\t\n\t\t\t'},{id:"B260",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tStark\n\t\t\t\t\t\t\tJ. D.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tBanks\n\t\t\t\t\t\t\tJ. 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J.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tPeveling\n\t\t\t\t\t\t\tR.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2011\n\t\t\t\t\tThe impact of the locust control insecticide fipronil on termites and ants in two contrasting habitats in northern Australia\n\t\t\t\t\tCrop Protection\n\t\t\t\t\t30\n\t\t\t\t\t7\n\t\t\t\t\t814\n\t\t\t\t\t825\n\t\t\t\t\n\t\t\t'},{id:"B263",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSterk\n\t\t\t\t\t\t\tG.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tBenuzzi\n\t\t\t\t\t\t\tM.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2004\n\t\t\t\t\tNuovi fitofarmaci, prove di tossicita sui bombi in serra\n\t\t\t\t\tColture Protette\n\t\t\t\t\t33\n\t\t\t\t\t1\n\t\t\t\t\t75\n\t\t\t\t\t77\n\t\t\t\t\n\t\t\t'},{id:"B264",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tStevens\n\t\t\t\t\t\t\tM. M.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tBurdett\n\t\t\t\t\t\t\tA. S.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMudford\n\t\t\t\t\t\t\tE. M.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tHelliwell\n\t\t\t\t\t\t\tS.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tDoran\n\t\t\t\t\t\t\tG.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2011\n\t\t\t\t\tThe acute toxicity of fipronil to two non-target invertebrates associated with mosquito breeding sites in Australia\n\t\t\t\t\tActa Tropica\n\t\t\t\t\t117\n\t\t\t\t\t2\n\t\t\t\t\t125\n\t\t\t\t\t130\n\t\t\t\t\n\t\t\t'},{id:"B265",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tStoughton\n\t\t\t\t\t\t\tSarah. J.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tKarsten\n\t\t\t\t\t\t\tLiber.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tJoseph\n\t\t\t\t\t\t\tCulp.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tAllan\n\t\t\t\t\t\t\tCessna.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2008\n\t\t\t\t\tAcute and chronic toxicity of imidacloprid to the aquatic invertebrates Chironomus tentans and Hyalella azteca under constant- and pulse-exposure conditions.\n\t\t\t\t\tArch. Environ. Contam. 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Entomol.\n\t\t\t\t\t94\n\t\t\t\t\t6\n\t\t\t\t\t1340\n\t\t\t\t\t1343\n\t\t\t\t\n\t\t\t'},{id:"B271",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tTanaka\n\t\t\t\t\t\t\tT.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMinakuchi\n\t\t\t\t\t\t\tC.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2011\n\t\t\t\t\tInsecticides and parasitoids.\n\t\t\t\t\tIn: Insecticides- Advances in Integrated Pest Management \nPerveen, F., editor, InTech, Rijeka, Croatia\n\t\t\t\t\t115\n\t\t\t\t\t140\n\t\t\t\t\n\t\t\t'},{id:"B272",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tTapparo\n\t\t\t\t\t\t\tA.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tGiorio\n\t\t\t\t\t\t\tC.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMarzaro\n\t\t\t\t\t\t\tM.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMarton\n\t\t\t\t\t\t\tD.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSolda\n\t\t\t\t\t\t\tL.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tGirolami\n\t\t\t\t\t\t\tV.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2011\n\t\t\t\t\tRapid analysis of neonicotinoid insecticides in guttation drops of corn seedlings obtained from coated seeds\n\t\t\t\t\tJ. 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Neurochem.\n\t\t\t\t\t75\n\t\t\t\t\t3\n\t\t\t\t\t1294\n\t\t\t\t\t1303\n\t\t\t\t\n\t\t\t'},{id:"B308",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tZhou\n\t\t\t\t\t\t\tS.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tDong\n\t\t\t\t\t\t\tQ.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tLi\n\t\t\t\t\t\t\tS.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tGuo\n\t\t\t\t\t\t\tJ.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tWang\n\t\t\t\t\t\t\tX.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tZhu\n\t\t\t\t\t\t\tG.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2009\n\t\t\t\t\tDevelopmental toxicity of cartap on zebrafish embryos\n\t\t\t\t\tAquat. Toxicol.\n\t\t\t\t\t95\n\t\t\t\t\t4\n\t\t\t\t\t339\n\t\t\t\t\t346\n\t\t\t\t\n\t\t\t'}],footnotes:[],contributors:[{corresp:"yes",contributorFullName:"Francisco Sánchez-Bayo",address:" sanchezbayo@mac.com",affiliation:'
University of Technology Sydney, Australia
'},{corresp:null,contributorFullName:"Henk A. Tennekes",address:null,affiliation:'
Experimental Toxicology Services (ETS) Nederland BV, The Netherlands
National Institute for Environmental Sciences, Japan
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Cuervo-Parra, Teresa Romero Cortés and Mario Ramirez-\nLepe",authors:[{id:"76645",title:"Dr.",name:"Mario",middleName:null,surname:"Ramirez-Lepe",fullName:"Mario Ramirez-Lepe",slug:"mario-ramirez-lepe"},{id:"177394",title:"Dr.",name:"Jaime Alioscha",middleName:null,surname:"Cuervo-Parra",fullName:"Jaime Alioscha Cuervo-Parra",slug:"jaime-alioscha-cuervo-parra"},{id:"177395",title:"Dr.",name:"Teresa",middleName:null,surname:"Romero-Cortes",fullName:"Teresa Romero-Cortes",slug:"teresa-romero-cortes"}]},{id:"49420",title:"Resistance and Its Management to Microbial and Insect Growth Regulator Larvicides in Mosquitoes",slug:"resistance-and-its-management-to-microbial-and-insect-growth-regulator-larvicides-in-mosquitoes",signatures:"Tianyun Su",authors:[{id:"176406",title:"Ph.D.",name:"Tianyun",middleName:null,surname:"Su",fullName:"Tianyun Su",slug:"tianyun-su"}]},{id:"49174",title:"Optimizing Strategic Insecticide Resistance Management Planning in Malaria Vectors",slug:"optimizing-strategic-insecticide-resistance-management-planning-in-malaria-vectors",signatures:"Emmanuel Chanda",authors:[{id:"175938",title:"Dr.",name:"Emmanuel",middleName:null,surname:"Chanda",fullName:"Emmanuel Chanda",slug:"emmanuel-chanda"}]},{id:"49270",title:"Insecticide Resistance in East Africa — History, Distribution and Drawbacks on Malaria Vectors and Disease Control",slug:"insecticide-resistance-in-east-africa-history-distribution-and-drawbacks-on-malaria-vectors-and-dise",signatures:"Delenasaw Yewhalaw and Eliningaya J. Kweka",authors:[{id:"123576",title:"Prof.",name:"Eliningaya",middleName:null,surname:"Kweka",fullName:"Eliningaya Kweka",slug:"eliningaya-kweka"},{id:"175958",title:"Dr.",name:"Delenasaw",middleName:null,surname:"Yewhalaw",fullName:"Delenasaw Yewhalaw",slug:"delenasaw-yewhalaw"}]},{id:"49298",title:"Emerging Insect-Borne Diseases of Agricultural, Medical and Veterinary Importance",slug:"emerging-insect-borne-diseases-of-agricultural-medical-and-veterinary-importance",signatures:"Marcello Nicoletti, Kadarkarai Murugan and Giovanni Benelli",authors:[{id:"65501",title:"Prof.",name:"Marcello",middleName:null,surname:"Nicoletti",fullName:"Marcello Nicoletti",slug:"marcello-nicoletti"}]},{id:"49941",title:"Insecticide Resistance and Fitness Cost",slug:"insecticide-resistance-and-fitness-cost",signatures:"Thiago Affonso Belinato and Ademir Jesus Martins",authors:[{id:"83198",title:"Prof.",name:"Ademir",middleName:"Jesus",surname:"Martins",fullName:"Ademir Martins",slug:"ademir-martins"}]},{id:"49385",title:"A Review of Insecticide Resistance Status in Botswana",slug:"a-review-of-insecticide-resistance-status-in-botswana",signatures:"N.M. Makate",authors:[{id:"176112",title:"Dr.",name:"Ntebaleng",middleName:null,surname:"Makate",fullName:"Ntebaleng Makate",slug:"ntebaleng-makate"}]},{id:"49354",title:"Effect of Imidacloprid on Bacterial Soil Isolate Bacillus weihenstephanensis",slug:"effect-of-imidacloprid-on-bacterial-soil-isolate-bacillus-weihenstephanensis",signatures:"A.A. Shetti and B.B. Kaliwal",authors:[{id:"78330",title:"Dr.",name:"Basappa",middleName:null,surname:"Kaliwal",fullName:"Basappa Kaliwal",slug:"basappa-kaliwal"}]},{id:"49288",title:"Resistance in Bacteria",slug:"resistance-in-bacteria",signatures:"S.O. Sadashiv and Basappa B. Kaliwal",authors:[{id:"78330",title:"Dr.",name:"Basappa",middleName:null,surname:"Kaliwal",fullName:"Basappa Kaliwal",slug:"basappa-kaliwal"},{id:"176334",title:"Dr.",name:"Sadashiv",middleName:null,surname:"S. O.",fullName:"Sadashiv S. O.",slug:"sadashiv-s.-o."}]},{id:"49761",title:"The Role of Glutathione Transferases in the Development of Insecticide Resistance",slug:"the-role-of-glutathione-transferases-in-the-development-of-insecticide-resistance",signatures:"Zazali Alias",authors:[{id:"176212",title:"Dr.",name:"Zazali",middleName:null,surname:"Alias",fullName:"Zazali Alias",slug:"zazali-alias"}]},{id:"49576",title:"Biological and Biochemical Bases of Pesticides Resistance in Rhipicephalus (Boophilus) microplus",slug:"biological-and-biochemical-bases-of-pesticides-resistance-in-rhipicephalus-boophilus-microplus",signatures:"Rodrigo Rosario-Cruz and Delia Inés Domínguez-García",authors:[{id:"176211",title:"Ph.D.",name:"Rodrigo",middleName:null,surname:"Rosario-Cruz",fullName:"Rodrigo Rosario-Cruz",slug:"rodrigo-rosario-cruz"},{id:"176225",title:"Dr.",name:"Delia Ines",middleName:null,surname:"Domínguez-García",fullName:"Delia Ines Domínguez-García",slug:"delia-ines-dominguez-garcia"}]},{id:"49628",title:"Biochemical Insecticide Resistance in Tea Pests",slug:"biochemical-insecticide-resistance-in-tea-pests",signatures:"Dhiraj Saha",authors:[{id:"176292",title:"Dr.",name:"Dhiraj",middleName:null,surname:"Saha",fullName:"Dhiraj Saha",slug:"dhiraj-saha"}]},{id:"49233",title:"The Role of Volatile Substances Emitted by Cultivated Plant's Roots in Indirect Defense Against Soil Herbivores",slug:"the-role-of-volatile-substances-emitted-by-cultivated-plant-s-roots-in-indirect-defense-against-soil",signatures:"Žiga Laznik and Stanislav Trdan",authors:[{id:"78285",title:"Prof.",name:"Stanislav",middleName:null,surname:"Trdan",fullName:"Stanislav Trdan",slug:"stanislav-trdan"},{id:"78340",title:"Dr.",name:"Žiga",middleName:null,surname:"Laznik",fullName:"Žiga Laznik",slug:"ziga-laznik"}]},{id:"49223",title:"About Previous Investigations Regarding the Role of Glucosinolates in Controlling Brassica Insect Pests in 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1. Introduction
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Myelodysplastic syndromes (MDS) are characterized by peripheral blood cytopenias secondary to the ineffective hematopoiesis, and represent a heterogeneous group of clonal hematologic malignancies in which abnormal multipotent progenitor cells are involved. As a result, there is an increased risk of bleeding diathesis and anemia requiring frequent transfusions, infections, and progression to acute myeloid leukemia [1, 2, 3, 4]. It is a very well-known fact that a large spectrum of genetic mutations is involved in MDS pathogenesis that may affect clinical outcome and response to the treatment. These genetic mutations may control cell cycle by affecting key proteins of spliceosome, DNA repair, kinase signaling, tumor suppressor genes, and transcription factors, changing bone marrow micro environment, resulting in hypercellular bone marrow with peripheral cytopenias through enhanced programmed cell death (PCD) and bone marrow dysfunction [5, 6]. To overcome programmed cell death, hematopoietic growth factors such as erythropoiesis stimulating agents (ESAs) and granulocyte colony stimulating factor (G-CSF) are the 1st step in management of the low-grade MDS recommended by the American Society of Clinical Oncology (ASCO) to reduce early apoptosis [1].
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Thrombopoietic stimulating agents (TSAs), ESAs, G-CSF, antithymocyte globulin (ATG) [7], lenalidomide [8], and hypomethylating agents are some of the non-transplantation options for management of the MDS patients suggesting that immune dysregulation plays a pivotal role in MDS pathogenesis [1]. Although its etiology is not clear, it has been shown that natural killer (NK) cell activity and its response to chemokines is decreased in MDS, and natural killer cells will be progressively more dysfunctional with MDS progression [9]. It has been shown that although dysfunctional regulatory T-cells (Treg), cells in charge of suppressing T helper (Th) activity, contribute in early stages of MDS, Th expands in the later stages of MDS, and there function is significantly reduced with treatment [10].
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Tumor necrosis factor alpha (TNF-α) level is higher in bone marrow and peripheral blood plasma of MDS patients, and may reflect an unfavorable outcome [11, 12]. Study shows that plasma level of 19 cytokines are significantly altered compared with normal individuals, among all of them, C-X-C motif chemokine 10 (CXCL10) and interleukin 6 were associated with shortened survival [13]. The relationship between shorter survival and interleukin 6 levels is very well known, and high producing genotypes of both TNF-α and interleukin 6 are highly associated with transfusion dependency for both anemia and thrombocytopenia, and severity of the bicytopenias [14]. Interferon regulatory factor-1 (IRF-1), a transcriptional activator of interferon system, has anti oncogenic properties, inhibits tumor formation, and regulates innate immune response. It has been shown that IRF-1 mRNA is 10 fold decreased in MDS patients, while it is increased in MDS patients with autoimmune disorders, showing that IRF-1 may promote inflammation and autoimmunity and has a protective roll in MDS patient’s [15].
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Although it has been reported that MDS is significantly associated with autoimmune disorders, it may occur secondary to the autoimmune disorders per se [16] or exposure to the therapeutic agents used for treatment of autoimmune disorders. A retrospective study in Sweden on 1662 MDS patients and 42,878 matched controls revealed that underlying autoimmune disorder increased risk of MDS by 2.1 with highest risks observed with prior autoimmune hemolytic anemia (AIHA), polyarteritis nodosa (PAN), granulomatosis with polyangiitis (GPA), giant cell arteritis (GCA) and aplastic anemia. It was speculated that chronic stimulation of the immune system may act as a trigger and prone the patient to MDS [17]. In a retrospective study of 2471 patients, it was found that MDS occurred subsequent to autoimmune disorders, most commonly rheumatoid arthritis, Sjogren’s syndrome, systemic lupus erythematosus, polyarteritis nodosa, discoid lupus erythematosus, and pernicious anemia were associated with MDS [18]. In a case control study of 80 for MDS patients, it was found that there is strong and statistically significant evidence that MDS occurred after autoimmune disorders with Grave’s disease and Hashimoto’s thyroiditis were among the most important disorders [19]. There is a case report of limited granulomatosis with polyangiitis (GPA) treated with corticosteroids, who did develop myelodysplastic syndrome, papillary thyroid carcinoma, and gastric adenocarcinoma [20].
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There are several reports regarding therapy related MDS in population with autoimmune disorders who underwent disease modifying antirheumatic drugs (DMARDs) therapy. In a retrospective case-control study of 40,011 patients with 27 kinds of autoimmune disorders, 311 patients met the inclusion criteria, 86 of them had MDS, and found azathioprine exposure has increased 7 fold myeloid neoplasms in a median of 8 years. In this study, methotrexate, and mycophenolate mofetil did not elevate the risk of myeloid neoplasms [21]. However, there is a case report regarding association of low-dose oral methotrexate therapy with MDS in a rheumatoid arthritis patient [22]. In another study, from 370 rheumatologic patients who received azathioprine for at least 1 year, 59 patients underwent bone marrow examination and 2 of them found to have MDS. This study revealed that risk of secondary MDS is 100 fold higher in patients who received azathioprine for their rheumatologic disorders. Chromosomal examination of the patients with MDS secondary to the azathioprine showed abnormalities of chromosome 7 in majority of them (8 out of 10) [23]. There is also evidence that MDS patients who have autoimmune disorders might respond to hypomethylating agents such as azacitidine and decitabine [24].
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2. Rheumatologic manifestations of MDS
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It is a very well-known fact that about 10–30% of MDS patients may present with a variety of autoimmune or laboratory manifestations or develop an autoimmune disorder. These manifestations are polymorphic and include leukocytoclastic vasculitis, clubbing, peripheral neuropathy, autoimmune hemolytic anemia, polyarthritis, myositis, acute or systemic vasculitis, Raynaud’s phenomenon, polyarteritis nodosa, vitiligo, iritis, colonic ulcerations, pulmonary involvement, and reported autoimmune disorders include rheumatoid arthritis, Sjogren’s disease, giant cell arteritis, polymyalgia rheumatica, relapsing polychondritis, Behçet’s disease, and systemic lupus erythematosus. Although autoimmune manifestations are mostly seen during the course of MDS, autoimmune disorders may occur before MDS diagnosis. Laboratory manifestations of MDS include hypergammaglobulinemia, hypogammaglobulinemia, monoclonal gammopathy, positive direct antiglobulin test (DAT), positive ANA, rheumatoid factor, cryoglobulinemia, and anti-double-stranded DNA.
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3. Autoimmune disorders in myelodysplastic syndrome
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Several studies support that autoimmune disorders may occur in the setting of MDS, and treating MDS with immunosuppressors may improve the autoimmune disorder. Although there were several case reports of MDS preceding autoimmune disorders, the first comprehensive retrospective study in 1986 on 104 MDS patients revealed two patients had pernicious anemia, two had hypothyroidism, and one had both pernicious anemia and hypothyroidism [23]. Later, in a retrospective study in 1994, five patients with MDS reviewed and revealed polyarthritis with positive rheumatoid factor (RF) and necrotizing vasculitis [25]. In another retrospective study in 1995, 221 patients with MDS reviewed and found 30 patients with autoimmune disorders, and categorized patient to three categories of acute systemic vasculitis or autoimmune disorder, chronic or isolated autoimmune phenomena, and classic connective tissue disorders. Skin vasculitis, arthritis, and fever were among the most common autoimmune manifestations [26].
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A case series in 2002 showed various autoimmune paraneoplastic disorders including vasculitis, pyoderma gangrenosum, Coombs negative autoimmune hemolytic anemia, autoimmune thrombocytopenia, and chronic inflammatory demyelinating polyneuropathy (CIDP) with good response to immunosuppressive therapy [27]. In a retrospective review of 235 MDS patients autoimmune manifestations such as skin vasculitis (24%), noninfectious fever (13%), arthralgia and arthritis (13%), peripheral neuropathy (10%), and pulmonary infiltrates (8%) were more common than systemic vasculitis [28]. In a cohort of 1408 patients with MDS, 391 (28%) had autoimmune disorders, with hypothyroidism (44%), as the most prevalent, and idiopathic thrombocytopenic purpura (12%), rheumatoid arthritis (10%), and psoriasis (7%) were among the common manifestations of autoimmune diseases [29].
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In a retrospective study of 67 MDS patients with autoimmune diseases, neutrophilic dermatosis was the most common autoimmune disease (35.8%), followed by Behçet’s disease (14.9%), and rheumatoid arthritis (13.4%) [30].
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3.1. Vasculitis
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The association between MDS and vasculitis is rare, but more common than solid tumors, and has been described for decades. Cutaneous vasculitis presents by palpable purpura mainly in lower extremities that involves small vessels and is characterized by perivascular inflammation and vessel wall damage by infiltrating neutrophils. Although both cutaneous and systemic vasculitis has been reported in MDS patients, at times they can be seen together in MDS patients. For instance, in a case series of 6 biopsy proven cutaneous vasculitis patients with MDS, 3 patients had evidence of systemic vasculitis [31]. In one case, MDS patient with biopsy proven cutaneous vasculitis developed acute myeloid leukemia within 4 months of vasculitis diagnosis [32]. Henoch-Schonlein purpura, a small vessel vasculitis with IgA dominant immune deposits has been described in MDS patients [33].
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The association of MDS and polyarteritis nodosa (PAN) type medium-vessel vasculitis has been reported [34]. In a retrospective study of 8 patients with chronic myelomonocytic leukemia (CMML), with vasculitis involved the medium-vessel, fulfilling the criteria for classic PAN, the presentation was non-specific, and patients developed atypical manifestations [35]. There is a case report of 43-year-old man who qualified for a diagnosis of PAN and developed systemic vasculitis at the time of chronic myelomonocytic leukemia (CMML) diagnosis [36]. There is a report of two cases with CMML who presented with PAN-like systemic vasculitis with bilateral perirenal hemorrhage and negative antineutrophil cytoplasmic antibody with improvement of vasculitis with systemic steroids [37].
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Although most MDS associated vasculitis described as leukocytoclastic vasculitis, published case reports documented large vessel vasculitis as autoimmune complication of MDS. Aortitis has been reported as an autoimmune manifestation of an MDS patient at presentation [38]. There is a report of two MDS cases that presented with acute large vessel vasculitis with rapid improvement with systemic steroids [39]. In a retrospective analysis of 271 temporal arteritis patients, it was found that 20 patients had malignancy, of which 11 patients had MDS, favoring a relationship between large vessel vasculitis and MDS [40]. There is a case report of Takayasu’s arteritis diagnosed shortly after diagnosis of MDS, with progression to AML regardless of improvement of vasculitis with immunosuppressive treatment [41]. There is a case report of a 71-year-old woman presenting with fever, neck pain, anemia, and thrombocytopenia, with positive positron emission tomography (PET)/CT scan of the aorta and carotid arteries with negative temporal artery biopsy who received the diagnosis of MDS after a bone marrow aspiration analysis [42, 43].
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3.2. Behçet’s disease
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A multisystem, chronic inflammatory disease of unknown etiology, Behçet’s disease (BD) is characterized by recurrent oral and genital ulcers, uveitis, arthritis, and vascular involvement of several organs including pulmonary, central nervous system and gastrointestinal tract. In a retrospective study of 805 BD patients, 16 patients had MDS, from which 43.8% had BD prior to MDS, 18.7% diagnosed after MDS and 37.5% had concurrent BD and MDS. It has been shown that trisomy 8 has been accumulated in all of BD patients with MDS, and these patients more likely to be female, older age, and have fever and ileocecal ulcerations [44]. In a retrospective study of 46 MDS patients, 8 patients had trisomy 8, 5 of them had multiple intestinal ulcers, a common feature of BD. Two of the MDS patients with trisomy 8 and multiple intestinal ulcers were treated with granulocyte-colony stimulating factor (G-CSF), aggravating their symptoms, suggesting G-CSF should be used cautiously in this subgroup of MDS patients [45]. There is several case reports of BD associated with MDS [46, 47, 48, 49]. In a case report of two patients with BD and MDS, it has been suggested that PET/CT may help diagnosis of both BD and MDS with high uptake by bone marrow in MDS patients and genital and gastrointestinal aphthous ulcers in BD patients [50]. It has been suggested that the frequency of gastrointestinal involvement is more common in MDS-associated BD patients than general BD population [51].
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3.3. Inflammatory arthritis
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There are several case reports and small series of inflammatory arthritis and MDS co-occurrence. In a retrospective study of 28 MDS patients, 8 had acute seronegative inflammatory arthritis with good response of arthritis to steroids [52]. In a French multicenter retrospective study of 22 patients with MDS, 77% of patients had polyarthritis, and 68% had symmetric joint involvement. Radiologic erosions are rare, and MDS associated arthritis is more frequent in refractory anemia with excess blast (RAEB) [53].
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3.4. Miscellaneous
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There are several case reports of relapsing polychondritis (RP) presenting as a paraneoplastic disorder in the setting of MDS [54, 55]. In a retrospective study of hematological changes in 19 patients with relapsing polychondritis, MDS was found in three RP patients [56]. Autoimmune hemolytic anemia has been reported in association with MDS [57, 58, 59]. Association of systemic lupus erythematosus and MDS has been reported [60, 61, 62].
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3.5. Immunological abnormalities
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It is a very well-known fact that a spectrum of immunological abnormalities occur in MDS. In a retrospective study of 104 MDS patients, 12.5% had monoclonal gammopathy, 19% had low immunoglobulin levels, 32% had polyclonal rise in serum immunoglobulin level, and 8.1% had positive direct antiglobulin test (DAT) [25]. In a case series of 142 patients with MDS and CMML, 23.2% had non-organ specific autoantibody ANA as the most frequent serologic finding [25]. Thrombocytopenia is a common finding in MDS, and can be seen in up to two third of the patients. In a study of 54 MDS patients with no treatment of transfusions, direct platelet immunofluorescence test for platelet associated IgG was positive in 28 patients. Patients with higher amount of platelet associated IgG, had significantly higher mean platelet volume (MPV), thrombocytopenia and worse outcome [63].
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4. Prognosis
\n
It has been suggested that appearance of skin vasculitis in MDS patients may reflect acute myeloid leukemia transformation. In a prospective study of 157 MDS patients for a median of 44 months, 15 patients (9.55%) experienced skin lesions, and neutrophilic dermatosis (7, 4.46%), specific lesions (5, 3.18%), cutaneous vasculitis (2, 1.27%) and Behçet’s disease (1, 0.63%) were reported. This study revealed that neutrophilic dermatosis was more prevalent in MDS patients, may confer the higher risk of acute myeloid leukemia transformation [64]. In another study of 84 newly diagnosed MDS patients, correlation of cutaneous findings with immunologic parameters and prognostic features of MDS examined, and revealed that 21 patients had skin lesions at presentation, and skin manifestations were a significant predictor of the high-risk MDS subgroup [65].
\n
In a retrospective study of 153 MDS patients, 12% had autoimmune diseases, and 63% has at least one immunological abnormality in test results. In this study, the survival of patients without autoimmune diseases was better than patients with autoimmune disease [66]. However, in a 4 year prospective study of 70 MDS patients, 53 patients without and 13 patients with autoimmune disease, there was no particular difference concerning prognosis between two groups. And patients with autoimmune diseases were not statistically different in survival compared with MDS patients without autoimmune disease [67].
\n
\n
\n
5. Treatment
\n
Autoimmune disorders associated with MDS may predate or occur after MDS diagnosis, and their treatment many be associated with significant side effects in MDS patients. It has been recognized from several case reports that treatment of the underlying MDS may resolve the associated autoimmune disorders. In a transfusion dependent MDS patient who was receiving G-CSF and erythropoietin, neutrophilic dermatosis did not improve with G-CSF withholding. Two months after starting 5-azacitidine, a hypomethylating agent, the skin rash completely resolved, and did not recur after 2 years [68]. In another case series of 3 MDS patients with autoimmune disorders, 5-azacitidine improved both MDS and autoimmune disorders although long term steroid could not be tapered [69].
\n
In a retrospective study of 123 MDS patients with autoimmune disorders, 118 patients (96%) were treated with steroids, and 48% of patients were required a second line treatment for refractory disease or relapse. Although autoimmune disorder treatment did not improve MDS, MDS treatment with 5-azacitidine improved the autoimmune disorder in 9 out of 11 (80%) of patients [70]. In another retrospective study of 123 MDS/CMML patients with autoimmune disorders, 28 patients received at least 5 cycles of azacitidine, 20 of them did not respond to steroids. In 86% of MDS/CMML patients, clinical autoimmune syndromes improved by azacitidine, and prednisone dose tapered in 64% of patients [71].
\n
The overall effect of biologic medications efficacy in MDS patients who presented with autoimmune disorder is not clear. As of today, there is only one retrospective study of MDS patients with autoimmune disorders and biologic medications. In this study of at least one biologic medication, 29 patients followed for at least 3 years. 89% of patients received a biologic after failure or intolerance of two disease modifying anti rheumatic agents (DMARDs), however, 11% of patients received biologics as a first line treatment. Except rituximab, a CD-20 blocker, mainly for vasculitis (58% response), there was partial or insufficient response to TNF-α antagonists, and their efficacy is much less in autoimmune disorders associated with MDS than autoimmune disorders without MDS. Overall, response rate to 5-azacitidine in MDS-associated autoimmune disorders was 67% in favor of a causality relationship between MDS and autoimmune disorders [72].
\n
\n
\n
6. Conclusion
\n
Myelodysplastic syndromes are a heterogeneous group of progressive clonal hematopoietic stem cell disorders characterized by a varying degree of peripheral cytopenia, and increased probability of transformation to acute myeloid leukemia. MDS and particularly CMML are frequently associated with a variety of autoimmune disorders that can be diagnosed concomitantly with MDS or before or after MDS. The heterogeneity and complexity of pathology, clinical manifestations, response to therapy, and prognosis of MDS and its immune dysregulation makes the prognosis of MDS with autoimmune diseases a matter of debate. Prospective, randomized studies are required to confirm the autoimmune diseases role in MDS prognosis.
\n
\n
Acknowledgments
\n
The authors would like to thank Mr. Dixon Bennet for his technical assistance.
\n
\n
Disclosure
\n
None.
\n
\n',keywords:"autoimmune disorders (ADs), myelodysplastic syndrome (MDS), chronic myelomonocytic leukemia (CMML), Behçet’s disease, systemic lupus erythematosus (SLE), azathioprine, azacitidine, tumor necrosis factor alpha (TNF-α), regulatory T-cells (Treg), autoimmune hemolytic anemia (AIHA), vasculitis, chronic inflammatory demyelinating polyneuropathy (CIDP), neutrophilic dermatosis, Henoch-Schonlein purpura, relapsing polychondritis (RP), granulomatosis with polyangiitis (GPA), giant cell arteritis (GCA), polyarteritis nodosa (PAN)",chapterPDFUrl:"https://cdn.intechopen.com/pdfs/63270.pdf",chapterXML:"https://mts.intechopen.com/source/xml/63270.xml",downloadPdfUrl:"/chapter/pdf-download/63270",previewPdfUrl:"/chapter/pdf-preview/63270",totalDownloads:1215,totalViews:302,totalCrossrefCites:0,dateSubmitted:"April 10th 2018",dateReviewed:"August 4th 2018",datePrePublished:"November 5th 2018",datePublished:"February 20th 2019",dateFinished:"August 28th 2018",readingETA:"0",abstract:"Myelodysplastic syndromes are heterogeneous group of clonal hematologic malignancies characterized by peripheral blood cytopenias secondary to the ineffective hematopoiesis. ADs are frequently reported in MDS, the incidence ranging from 10 to 30%, and particularly ADs are more frequently seen at CMML. ADs may prone patient to MDS, especially when immune suppressors such as azathioprine are used for the underlying AD. Both innate and adaptive immune systems, and different cytokines including interleukins, TNF-α, and C-X-C motif chemokine 10 (CXCL10) contribute in immune dysregulation of MDS. Vasculitis, seronegative rheumatoid arthritis, SLE, Behçet’s disease, RP, and AIHA are just some of the ADs occurring concomitantly with MDS. Although hematopoietic growth factors are recommended by the American Society of Clinical Oncology (ASCO), it has been recognized from several case reports that treatment of the underlying MDS may resolve the associated autoimmune disorders. The heterogeneity and complexity of pathology, clinical manifestations, response to therapy, and prognosis of MDS and its immune dysregulation make the prognosis of MDS with autoimmune diseases a matter of debate. Better understanding of the immune dysregulation of MDS in the molecular level may help to design prospective, double blind clinical trials to find the best treatment options for autoimmune disorders associated with MDS.",reviewType:"peer-reviewed",bibtexUrl:"/chapter/bibtex/63270",risUrl:"/chapter/ris/63270",signatures:"Kam A. Newman, Mojtaba Akhtari and Sheda Heidarian",book:{id:"7138",type:"book",title:"Recent Developments in Myelodysplastic Syndromes",subtitle:null,fullTitle:"Recent Developments in Myelodysplastic Syndromes",slug:"recent-developments-in-myelodysplastic-syndromes",publishedDate:"February 20th 2019",bookSignature:"Ota Fuchs",coverURL:"https://cdn.intechopen.com/books/images_new/7138.jpg",licenceType:"CC BY 3.0",editedByType:"Edited by",isbn:"978-1-78985-478-7",printIsbn:"978-1-78985-477-0",pdfIsbn:"978-1-83962-040-9",isAvailableForWebshopOrdering:!0,editors:[{id:"36468",title:"Dr.",name:"Ota",middleName:null,surname:"Fuchs",slug:"ota-fuchs",fullName:"Ota Fuchs"}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"}},authors:[{id:"253996",title:"Dr.",name:"Kam A.",middleName:null,surname:"Newman",fullName:"Kam A. Newman",slug:"kam-a.-newman",email:"knewman2@emc.org",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:{name:"Eisenhower Medical Center",institutionURL:null,country:{name:"United States of America"}}},{id:"264133",title:"Dr.",name:"Mojtaba",middleName:null,surname:"Akhtari",fullName:"Mojtaba Akhtari",slug:"mojtaba-akhtari",email:"mojtaba.akhtari@med.usc.edu",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:null},{id:"264161",title:"Dr.",name:"Sheda",middleName:null,surname:"Heidarian",fullName:"Sheda Heidarian",slug:"sheda-heidarian",email:"SHeidarian@Eisenhowerhealth.org",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:null}],sections:[{id:"sec_1",title:"1. Introduction",level:"1"},{id:"sec_2",title:"2. Rheumatologic manifestations of MDS",level:"1"},{id:"sec_3",title:"3. Autoimmune disorders in myelodysplastic syndrome",level:"1"},{id:"sec_3_2",title:"3.1. Vasculitis",level:"2"},{id:"sec_4_2",title:"3.2. Behçet’s disease",level:"2"},{id:"sec_5_2",title:"3.3. Inflammatory arthritis",level:"2"},{id:"sec_6_2",title:"3.4. Miscellaneous",level:"2"},{id:"sec_7_2",title:"3.5. Immunological abnormalities",level:"2"},{id:"sec_9",title:"4. Prognosis",level:"1"},{id:"sec_10",title:"5. Treatment",level:"1"},{id:"sec_11",title:"6. Conclusion",level:"1"},{id:"sec_12",title:"Acknowledgments",level:"1"},{id:"sec_12",title:"Disclosure",level:"1"}],chapterReferences:[{id:"B1",body:'Newman K, Maness-Harris L, El-Hemaidi I, Akhtari M. Revisiting use of growth factors in myelodysplastic syndromes. Asian Pacific Journal of Cancer Prevention. 2012;13(4):1081-1091\n'},{id:"B2",body:'Sallman DA, Tanaka TN, List A, Bejar R. SOHO state of the art update and next questions: Biology and treatment of myelodysplastic syndromes. 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Platelet associated IgG may be related with thrombocytopenia in patients with myelodysplastic syndromes. Leukemia Research. 2012;36(5):554-559\n'},{id:"B64",body:'Farah C, Bulai Livideanu C, Jegu J, Paul C, Viraben R, Lamant L, et al. Prevalence and prognostic value of cutaneous manifestations in patients with myelodysplastic syndrome. Journal of the European Academy of Dermatology and Venereology. 2010;24(10):1171-1175\n'},{id:"B65",body:'Dalamaga M, Karmaniolas K, Matekovits A, Migdalis I, Papadavid E. Cutaneous manifestations in relation to immunologic parameters in a cohort of primary myelodysplastic syndrome patients. Journal of the European Academy of Dermatology and Venereology. 2008;22(5):543-548\n'},{id:"B66",body:'Okamoto T, Okada M, Mori A, Saheki K, Takatsuka H, Wada H, et al. Correlation between immunological abnormalities and prognosis in myelodysplastic syndrome patients. International Journal of Hematology. 1997;66(3):345-351\n'},{id:"B67",body:'Giannouli S, Voulgarelis M, Zintzaras E, Tzioufas AG, Moutsopoulos HM. Autoimmune phenomena in myelodysplastic syndromes: A 4-yr prospective study. Rheumatology (Oxford, England). 2004;43(5):626-632\n'},{id:"B68",body:'Raj K, Ho A, Creamer JD, du Vivier AW, Salisbury JR, Mufti GJ. Complete response of deep neutrophilic dermatosis associated with myelodysplastic syndrome to 5-azacytidine. The British Journal of Dermatology. 2007;156(5):1039-1041\n'},{id:"B69",body:'Pilorge S, Doleris LM, Dreyfus F, Park S. The autoimmune manifestations associated with myelodysplastic syndrome respond to 5-azacytidine: A report on three cases. British Journal of Haematology. 2011;153(5):664-665\n'},{id:"B70",body:'Mekinian A, Grignano E, Braun T, Decaux O, Liozon E, Costedoat-Chalumeau N, et al. Systemic inflammatory and autoimmune manifestations associated with myelodysplastic syndromes and chronic myelomonocytic leukaemia: A French multicentre retrospective study. Rheumatology (Oxford, England). 2016;55(2):291-300\n'},{id:"B71",body:'Fraison JB, Mekinian A, Grignano E, Kahn JE, Arlet JB, Decaux O, et al. Efficacy of Azacitidine in autoimmune and inflammatory disorders associated with myelodysplastic syndromes and chronic myelomonocytic leukemia. Leukemia Research. 2016;43:13-17\n'},{id:"B72",body:'Mekinian A, Dervin G, Lapidus N, Kahn JE, Terriou L, Liozon E, et al. GFM, SNFMI, CRI and MINHEMON. Biologics in myelodysplastic syndrome-related systemic inflammatory and autoimmune diseases: French multicenter retrospective study of 29 patients. Autoimmunity Reviews. 2017;16(9):903-910\n'}],footnotes:[],contributors:[{corresp:"yes",contributorFullName:"Kam A. Newman",address:"knewman@eisenhowerhealth.org",affiliation:'
Eisenhower Rheumatology Specialty Clinic, Eisenhower Health, Internal Medicine Residency Program, Mike and Jan Salta Health Center, United States
President Elect, Medical Staff, Eisenhower Health, United States
'}],corrections:null},book:{id:"7138",type:"book",title:"Recent Developments in Myelodysplastic Syndromes",subtitle:null,fullTitle:"Recent Developments in Myelodysplastic Syndromes",slug:"recent-developments-in-myelodysplastic-syndromes",publishedDate:"February 20th 2019",bookSignature:"Ota Fuchs",coverURL:"https://cdn.intechopen.com/books/images_new/7138.jpg",licenceType:"CC BY 3.0",editedByType:"Edited by",isbn:"978-1-78985-478-7",printIsbn:"978-1-78985-477-0",pdfIsbn:"978-1-83962-040-9",isAvailableForWebshopOrdering:!0,editors:[{id:"36468",title:"Dr.",name:"Ota",middleName:null,surname:"Fuchs",slug:"ota-fuchs",fullName:"Ota Fuchs"}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"}}},profile:{item:{id:"226652",title:"Dr.",name:"Terry J.",middleName:null,surname:"Ellapen",email:"tellapen1@yahoo.com",fullName:"Terry J. Ellapen",slug:"terry-j.-ellapen",position:null,biography:null,institutionString:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",totalCites:0,totalChapterViews:"0",outsideEditionCount:0,totalAuthoredChapters:"4",totalEditedBooks:"0",personalWebsiteURL:null,twitterURL:null,linkedinURL:null,institution:null},booksEdited:[],chaptersAuthored:[{id:"58562",title:"Biokinetics: A South African Health Profession Evolving from Physical Education and Sport",slug:"biokinetics-a-south-african-health-profession-evolving-from-physical-education-and-sport",abstract:"This chapter describes the South African profession of Biokinetics, which operates within the pathogenic and fortogenic health paradigms. Biokinetics is an exercise therapy profession that exclusively prescribes individulaised exercise and physical activity for rehabilitation and promotion of health and quality of life. Biokinetics differs from physiotherapy primarily due its management of injuries, illnesses and disabilities within the final-phase of rehabilitation. A brief history of the profession and its scope of profession and its alignment within the South African National Health statutory and professional bodies will be presented. The two pedagogic models adopted for the teaching and training of Biokinetics will also be discussed. Interprofessional collaborative partnerships within the medical-rehabilitation fraternity, sport, health and fitness industries and educational employment opportunities will be reviewed. Finally, the idea of internationalisation of the profession of Biokinetics to similar exercise therapy professions such as Clinical Exercise Physiology and Athletic Training will be presented.",signatures:"Terry Jeremy Ellapen, Gert Lukas Strydom, Mariette Swanepoel,\nHenriette Hammill and Yvonne Paul",authors:[{id:"127909",title:"Prof.",name:"Gert Lukas",surname:"Strydom",fullName:"Gert Lukas Strydom",slug:"gert-lukas-strydom",email:"gert.strydom@nwu.ac.za"},{id:"226652",title:"Dr.",name:"Terry J.",surname:"Ellapen",fullName:"Terry J. Ellapen",slug:"terry-j.-ellapen",email:"tellapen1@yahoo.com"},{id:"233593",title:"Dr.",name:"Mariette",surname:"Swanepoel",fullName:"Mariette Swanepoel",slug:"mariette-swanepoel",email:"12262404@nwu.ac.za"},{id:"233594",title:"Dr.",name:"Henriette Valerie",surname:"Hammill",fullName:"Henriette Valerie Hammill",slug:"henriette-valerie-hammill",email:"12782211@nwu.ac.za"},{id:"233596",title:"Prof.",name:"Yvonne",surname:"Paul",fullName:"Yvonne Paul",slug:"yvonne-paul",email:"pauly@tut.ac.za"}],book:{id:"6343",title:"Sport and Exercise Science",slug:"sport-and-exercise-science",productType:{id:"1",title:"Edited Volume"}}},{id:"71148",title:"A Conceptual Framework for the Inclusion of Recreational Therapy within South African Healthcare Paradigms",slug:"a-conceptual-framework-for-the-inclusion-of-recreational-therapy-within-south-african-healthcare-par",abstract:"Final-phase rehabilitation in South Africa is synonymous with the professions of Physiotherapy and Biokinetics; no consideration is given to the contribution of the profession of Recreational Therapy, which successfully contributes to other international healthcare paradigms. The primary aim was to determine whether collaborative relationships exist between South African recreational therapists and physiotherapists or biokineticists. A secondary aim was to review the potential inclusion of the profession of Recreational Therapy within the existing South African patient referral system of the multidisciplinary healthcare paradigm. An electronic search of the Google Scholar and Sabinet databases identified no records regarding interprofessional collaborative relationships between Recreational Therapy, Physiotherapy and Biokinetics. The authors therefore used two indirect records that prescribe interprofessional collaboration among South African healthcare practitioners during final-phase exercise rehabilitation. The quality of these individual records was appraised using the modified Downs and Black Scale in order to reduce bias. While there is a paucity of literature identifying the absence of interprofessional collaborative relationships between Recreational Therapy, Biokinetics, and Physiotherapy, the Health Professions Council of South Africa (HPCSA) guide nevertheless allows for dynamic overlap among final-phase exercise therapists, thereby providing an opportunity for the inclusion of Recreational Therapy within the existing dynamic, multidisciplinary, South African healthcare paradigm. The inclusion of the profession of Recreational Therapy, as part of a collaborative team effort, can be helpful in order to address the multifaceted challenges experienced by many South African patients.",signatures:"Terry Jeremy Ellapen, Mariette Swanepoel, Marco Barnard and Yvonne Paul",authors:[{id:"226652",title:"Dr.",name:"Terry J.",surname:"Ellapen",fullName:"Terry J. Ellapen",slug:"terry-j.-ellapen",email:"tellapen1@yahoo.com"},{id:"233593",title:"Dr.",name:"Mariette",surname:"Swanepoel",fullName:"Mariette Swanepoel",slug:"mariette-swanepoel",email:"12262404@nwu.ac.za"},{id:"233596",title:"Prof.",name:"Yvonne",surname:"Paul",fullName:"Yvonne Paul",slug:"yvonne-paul",email:"pauly@tut.ac.za"},{id:"316231",title:"Mr.",name:"Marco",surname:"Barnard",fullName:"Marco Barnard",slug:"marco-barnard",email:"marcobarnard37@gmail.com"}],book:{id:"7543",title:"Physical Therapy Effectiveness",slug:"physical-therapy-effectiveness",productType:{id:"1",title:"Edited Volume"}}},{id:"74116",title:"The Interprofessional Clinical and Therapeutic Team Strategy to Manage Spinal Cord Injuries",slug:"the-interprofessional-clinical-and-therapeutic-team-strategy-to-manage-spinal-cord-injuries",abstract:"A popular comorbidity of spinal cord injuries is physical deconditioning that frequently prejudice the person to increased risk for secondary non-communicable diseases, such as non-dependent insulin diabetes mellitus, cardiovascular diseases, respiratory diseases, cardiorespiratory diseases, obesity, osteoporosis, arthritis and osteoarthritis. Clinical literature has shown that spinal cord injured individuals have a poor cardiometabolic risk profile that amplifies the likelihood of secondary non-communicable diseases. Components of physical deconditioning include muscle atrophy, decreased aerobic capacity, inflexibility and diminished muscle and endurance. Another problem associated with spinal cord injuries is reliance or dependence on others. The combination of poor physical conditioning and dependence on others often adversely impacts on the individual’s quality of life, limiting their social interaction with others. The adherence to habitual physical activity and exercises has shown to increase conditioning status, improve health and wellbeing, increase independence, and improve confidence and self-image and successful re-integration in community. Therefore it is of paramount importance to increase awareness of the benefits of habitual physical activity and exercise to spinal cord injured patients, medical and clinical practitioners, family and friends. This chapter intends to highlight the health benefits of habitual physical activity in relation to selected secondary non-communicable diseases, and, the importance of interprofessional clinical and therapeutic team strategy to improve the spinal cord injured individuals’ quality of life.",signatures:"Adele Jordaan, Mariette Swanepoel, Yvonne Paul and Terry Jeremy Ellapen",authors:[{id:"226652",title:"Dr.",name:"Terry J.",surname:"Ellapen",fullName:"Terry J. Ellapen",slug:"terry-j.-ellapen",email:"tellapen1@yahoo.com"},{id:"233596",title:"Prof.",name:"Yvonne",surname:"Paul",fullName:"Yvonne Paul",slug:"yvonne-paul",email:"pauly@tut.ac.za"},{id:"331279",title:"Ms.",name:"Adele",surname:"Jordaan",fullName:"Adele Jordaan",slug:"adele-jordaan",email:"Adele3@gmail.com"},{id:"331280",title:"Prof.",name:"Mariëtte",surname:"Swanepoel",fullName:"Mariëtte Swanepoel",slug:"mariette-swanepoel",email:"swanepoelm@nwu.ac.za"}],book:{id:"10347",title:"Therapy Approaches in Neurological Disorders",slug:"therapy-approaches-in-neurological-disorders",productType:{id:"1",title:"Edited Volume"}}},{id:"76728",title:"The Profession of Biokinetics in South Africa: The Need for Access to the Public Healthcare System",slug:"the-profession-of-biokinetics-in-south-africa-the-need-for-access-to-the-public-healthcare-system",abstract:"This chapter reviews the efficacy of the only South African exercise therapy profession (Biokinetics) in the rehabilitation of non-communicable diseases (NCDs). Biokinetics is a South African exercise therapy profession established in 1983 and which operates in both the pathogenic and fortogenic healthcare paradigms. Unfortunately, the profession of Biokinetics is restricted to the South African private healthcare sector. This chapter describes the scope of the profession of Biokinetics, empirical studies illustrating the efficacy of the profession in addressing society’s non-communicable disease epidemic, and the challenges inhibiting the profession from gaining access to the South African public healthcare sector. It is hoped that the presentation and critical appraisal of the empirical evidence which illustrates the contribution of the profession of Biokinetics to the rehabilitation of NCDs justifies the authors’ claims for the inclusion of the aforementioned profession in the South African public healthcare sector.",signatures:"Yvonne Paul, Terry J. Ellapen, Takalani C. Muluvhu and Makwena B. Ntjana",authors:[{id:"226652",title:"Dr.",name:"Terry J.",surname:"Ellapen",fullName:"Terry J. Ellapen",slug:"terry-j.-ellapen",email:"tellapen1@yahoo.com"},{id:"233596",title:"Prof.",name:"Yvonne",surname:"Paul",fullName:"Yvonne Paul",slug:"yvonne-paul",email:"pauly@tut.ac.za"},{id:"414123",title:"Dr.",name:"Takalani C",surname:"Muluvhu",fullName:"Takalani C Muluvhu",slug:"takalani-c-muluvhu",email:"muluvhutc@tut.ac.za"},{id:"414124",title:"Mr.",name:"Makwena B.",surname:"Ntjana",fullName:"Makwena B. Ntjana",slug:"makwena-b.-ntjana",email:"Brinkntjana@gmail.com"}],book:{id:"10705",title:"Healthcare Access",slug:"healthcare-access",productType:{id:"1",title:"Edited Volume"}}}],collaborators:[{id:"144247",title:"Dr.",name:"Andrzej",surname:"Mroczkowski",slug:"andrzej-mroczkowski",fullName:"Andrzej Mroczkowski",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"University of Zielona Góra",institutionURL:null,country:{name:"Poland"}}},{id:"189960",title:"Prof.",name:"Sharon Kay",surname:"Stoll",slug:"sharon-kay-stoll",fullName:"Sharon Kay Stoll",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"University of Idaho",institutionURL:null,country:{name:"United States of America"}}},{id:"205593",title:"Dr.",name:"Heather",surname:"VanMullem",slug:"heather-vanmullem",fullName:"Heather VanMullem",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:null},{id:"211582",title:"Dr.",name:"Jorge",surname:"Sanhueza",slug:"jorge-sanhueza",fullName:"Jorge Sanhueza",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Universidad Mayor",institutionURL:null,country:{name:"Chile"}}},{id:"211583",title:"Dr.",name:"Andrew",surname:"Gardner",slug:"andrew-gardner",fullName:"Andrew Gardner",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"University of Wisconsin School of Medicine and Public Health",institutionURL:null,country:{name:"United States of America"}}},{id:"213781",title:"Dr.",name:"Pete",surname:"Van Mullem",slug:"pete-van-mullem",fullName:"Pete Van Mullem",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:null},{id:"213782",title:"Dr.",name:"Jennifer",surname:"Beller",slug:"jennifer-beller",fullName:"Jennifer Beller",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:null},{id:"218702",title:"Ph.D.",name:"Francisco J.",surname:"Sobrino",slug:"francisco-j.-sobrino",fullName:"Francisco J. Sobrino",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/218702/images/6351_n.jpg",biography:"Medical specialist in Traumatology and Orthopedic surgery, developing my professional career in the field of Sports & Dance Traumatology, Labor Traumatology, Arthroscopic surgery and musculoskeletal injuries Prevention. \nDoctor´s degree in Medicine and Surgery at the Complutense University of Madrid with the qualification of Outstanding Cum laude. Master in Musculoskeletal System Biomechanics and Medical Specialist in Clinical and Functional Anatomy of the Knee at the Complutense University of Madrid. Diploma at Musculoskeletal injuries prevention at Complutense University of Madrid and the Spanish National Health Institute. \nMore than 50 Papers and Conferences in different National and International Congresses related to Traumatology and Orthopedic Surgery.20 articles published in national and international books and Scientific Journals.4 awards at national and international Simposium and Congresses. \nMember of the Spanish Scientific Society of Traumotology and Orthopedic Surgery, Spanish Society of Sports Traumatology, Spanish Society of Labor Traumotology. \nMember of the International Dance Council (CID) UNESCO. \nI am currently Tutor Traumatologist at FREMAP Madrid, Head of the Health Department at Madrid City Council, External Consultant Traumatologist Advisor at the Quiron Group at the San Jose Clinic in Madrid, and at the Anglo American Medical Unit, also in Madrid. Likewise, I am also an External Consultant Traumatologist Advisor of dancers belonging to the main Spanish ballet companies.",institutionString:null,institution:null},{id:"221070",title:"MSc.",name:"Carlos",surname:"Bahamondes",slug:"carlos-bahamondes",fullName:"Carlos Bahamondes",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:null},{id:"221071",title:"Dr.",name:"Claudio",surname:"Hernandez Mosqueira",slug:"claudio-hernandez-mosqueira",fullName:"Claudio Hernandez Mosqueira",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:null}]},generic:{page:{slug:"content-alerts",title:"Content alerts",intro:"
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His studies in robotics lead him not only to a PhD degree but also inspired him to co-found and build the International Journal of Advanced Robotic Systems - world's first Open Access journal in the field of robotics.",institutionString:null,institution:{name:"TU Wien",country:{name:"Austria"}}},{id:"441",title:"Ph.D.",name:"Jaekyu",middleName:null,surname:"Park",slug:"jaekyu-park",fullName:"Jaekyu Park",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/441/images/1881_n.jpg",biography:null,institutionString:null,institution:{name:"LG Corporation (South Korea)",country:{name:"Korea, South"}}},{id:"465",title:"Dr",name:"Christian",middleName:null,surname:"Martens",slug:"christian-martens",fullName:"Christian Martens",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:null},{id:"479",title:"Dr.",name:"Valentina",middleName:null,surname:"Colla",slug:"valentina-colla",fullName:"Valentina Colla",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/479/images/358_n.jpg",biography:null,institutionString:null,institution:{name:"Sant'Anna School of Advanced Studies",country:{name:"Italy"}}},{id:"494",title:"PhD",name:"Loris",middleName:null,surname:"Nanni",slug:"loris-nanni",fullName:"Loris Nanni",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/494/images/system/494.jpg",biography:"Loris Nanni received his Master Degree cum laude on June-2002 from the University of Bologna, and the April 26th 2006 he received his Ph.D. in Computer Engineering at DEIS, University of Bologna. On September, 29th 2006 he has won a post PhD fellowship from the university of Bologna (from October 2006 to October 2008), at the competitive examination he was ranked first in the industrial engineering area. He extensively served as referee for several international journals. He is author/coauthor of more than 100 research papers. He has been involved in some projects supported by MURST and European Community. His research interests include pattern recognition, bioinformatics, and biometric systems (fingerprint classification and recognition, signature verification, face recognition).",institutionString:null,institution:null},{id:"496",title:"Dr.",name:"Carlos",middleName:null,surname:"Leon",slug:"carlos-leon",fullName:"Carlos Leon",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"University of Seville",country:{name:"Spain"}}},{id:"512",title:"Dr.",name:"Dayang",middleName:null,surname:"Jawawi",slug:"dayang-jawawi",fullName:"Dayang Jawawi",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"University of Technology Malaysia",country:{name:"Malaysia"}}},{id:"528",title:"Dr.",name:"Kresimir",middleName:null,surname:"Delac",slug:"kresimir-delac",fullName:"Kresimir Delac",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/528/images/system/528.jpg",biography:"K. Delac received his B.Sc.E.E. degree in 2003 and is currentlypursuing a Ph.D. degree at the University of Zagreb, Faculty of Electrical Engineering andComputing. His current research interests are digital image analysis, pattern recognition andbiometrics.",institutionString:null,institution:{name:"University of Zagreb",country:{name:"Croatia"}}},{id:"557",title:"Dr.",name:"Andon",middleName:"Venelinov",surname:"Topalov",slug:"andon-topalov",fullName:"Andon Topalov",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/557/images/1927_n.jpg",biography:"Dr. Andon V. Topalov received the MSc degree in Control Engineering from the Faculty of Information Systems, Technologies, and Automation at Moscow State University of Civil Engineering (MGGU) in 1979. He then received his PhD degree in Control Engineering from the Department of Automation and Remote Control at Moscow State Mining University (MGSU), Moscow, in 1984. From 1985 to 1986, he was a Research Fellow in the Research Institute for Electronic Equipment, ZZU AD, Plovdiv, Bulgaria. In 1986, he joined the Department of Control Systems, Technical University of Sofia at the Plovdiv campus, where he is presently a Full Professor. He has held long-term visiting Professor/Scholar positions at various institutions in South Korea, Turkey, Mexico, Greece, Belgium, UK, and Germany. And he has coauthored one book and authored or coauthored more than 80 research papers in conference proceedings and journals. His current research interests are in the fields of intelligent control and robotics.",institutionString:null,institution:{name:"Technical University of Sofia",country:{name:"Bulgaria"}}},{id:"585",title:"Prof.",name:"Munir",middleName:null,surname:"Merdan",slug:"munir-merdan",fullName:"Munir Merdan",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/585/images/system/585.jpg",biography:"Munir Merdan received the M.Sc. degree in mechanical engineering from the Technical University of Sarajevo, Bosnia and Herzegovina, in 2001, and the Ph.D. degree in electrical engineering from the Vienna University of Technology, Vienna, Austria, in 2009.Since 2005, he has been at the Automation and Control Institute, Vienna University of Technology, where he is currently a Senior Researcher. His research interests include the application of agent technology for achieving agile control in the manufacturing environment.",institutionString:null,institution:null},{id:"605",title:"Prof",name:"Dil",middleName:null,surname:"Hussain",slug:"dil-hussain",fullName:"Dil Hussain",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/605/images/system/605.jpg",biography:"Dr. Dil Muhammad Akbar Hussain is a professor of Electronics Engineering & Computer Science at the Department of Energy Technology, Aalborg University Denmark. Professor Akbar has a Master degree in Digital Electronics from Govt. College University, Lahore Pakistan and a P-hD degree in Control Engineering from the School of Engineering and Applied Sciences, University of Sussex United Kingdom. Aalborg University has Two Satellite Campuses, one in Copenhagen (Aalborg University Copenhagen) and the other in Esbjerg (Aalborg University Esbjerg).\n· He is a member of prestigious IEEE (Institute of Electrical and Electronics Engineers), and IAENG (International Association of Engineers) organizations. \n· He is the chief Editor of the Journal of Software Engineering.\n· He is the member of the Editorial Board of International Journal of Computer Science and Software Technology (IJCSST) and International Journal of Computer Engineering and Information Technology. \n· He is also the Editor of Communication in Computer and Information Science CCIS-20 by Springer.\n· Reviewer For Many Conferences\nHe is the lead person in making collaboration agreements between Aalborg University and many universities of Pakistan, for which the MOU’s (Memorandum of Understanding) have been signed.\nProfessor Akbar is working in Academia since 1990, he started his career as a Lab demonstrator/TA at the University of Sussex. After finishing his P. hD degree in 1992, he served in the Industry as a Scientific Officer and continued his academic career as a visiting scholar for a number of educational institutions. In 1996 he joined National University of Science & Technology Pakistan (NUST) as an Associate Professor; NUST is one of the top few universities in Pakistan. In 1999 he joined an International Company Lineo Inc, Canada as Manager Compiler Group, where he headed the group for developing Compiler Tool Chain and Porting of Operating Systems for the BLACKfin processor. The processor development was a joint venture by Intel and Analog Devices. In 2002 Lineo Inc., was taken over by another company, so he joined Aalborg University Denmark as an Assistant Professor.\nProfessor Akbar has truly a multi-disciplined career and he continued his legacy and making progress in many areas of his interests both in teaching and research. He has contributed in stochastic estimation of control area especially, in the Multiple Target Tracking and Interactive Multiple Model (IMM) research, Ball & Beam Control Problem, Robotics, Levitation Control. He has contributed in developing Algorithms for Fingerprint Matching, Computer Vision and Face Recognition. He has been supervising Pattern Recognition, Formal Languages and Distributed Processing projects for several years. He has reviewed many books on Management, Computer Science. Currently, he is an active and permanent reviewer for many international conferences and symposia and the program committee member for many international conferences.\nIn teaching he has taught the core computer science subjects like, Digital Design, Real Time Embedded System Programming, Operating Systems, Software Engineering, Data Structures, Databases, Compiler Construction. 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Morales and Sagrario Martínez-Ramírez",authors:[{id:"107401",title:"Dr.",name:"Lucia J",middleName:null,surname:"Fernández",slug:"lucia-j-fernandez",fullName:"Lucia J Fernández"}]},{id:"53128",doi:"10.5772/66368",title:"Phenolic Compounds: Functional Properties, Impact of Processing and Bioavailability",slug:"phenolic-compounds-functional-properties-impact-of-processing-and-bioavailability",totalDownloads:9318,totalCrossrefCites:75,totalDimensionsCites:142,abstract:"In this chapter, we discuss the influence of the processing methods on the content of phenolic compounds in fruits and vegetables. The intake of fruits and vegetables based‐foods are associated with delayed aging and a decreased risk of chronic disease development. Fruits and vegetables can be consumed in natura, but the highest amounts are ingested after some processing methods, such as cooking procedures or sanitizing methods. These methods are directly methods are directly related to alteration on the phenolic content. In addition, the postharvest conditions may modify several phytochemical substances. Phenolic compounds are referred to as phytochemicals found in a large number of foods and beverages. The relative high diversity of these molecules produced by plants must be taken into account when methods of preparation are employed to obtain industrial or homemade products. Phenolic compounds comprise one (phenolic acids) or more (polyphenols) aromatic rings with attached hydroxyl groups in their structures. Their antioxidant capacities are related to these hydroxyl groups and phenolic rings. Despite the antioxidant activity, they have many other beneficial effects on human health. However, before attributing health benefits to these compounds, absorption, distribution, and metabolism of each phenolic compound in the body are important points that should be considered.",book:{id:"5609",slug:"phenolic-compounds-biological-activity",title:"Phenolic Compounds",fullTitle:"Phenolic Compounds - Biological Activity"},signatures:"Igor Otavio Minatel, Cristine Vanz Borges, Maria Izabela Ferreira,\nHector Alonzo Gomez Gomez, Chung-Yen Oliver Chen and\nGiuseppina Pace Pereira Lima",authors:[{id:"146379",title:"Dr.",name:"Giuseppina",middleName:null,surname:"Lima",slug:"giuseppina-lima",fullName:"Giuseppina Lima"},{id:"194002",title:"MSc.",name:"Cristine",middleName:null,surname:"Vanz Borges",slug:"cristine-vanz-borges",fullName:"Cristine Vanz Borges"},{id:"194003",title:"Prof.",name:"Igor Otavio",middleName:null,surname:"Minatel",slug:"igor-otavio-minatel",fullName:"Igor Otavio Minatel"},{id:"194004",title:"Dr.",name:"Maria Izabela",middleName:null,surname:"Ferreira",slug:"maria-izabela-ferreira",fullName:"Maria Izabela Ferreira"},{id:"194005",title:"Prof.",name:"Hector",middleName:null,surname:"Gomez-Gomez",slug:"hector-gomez-gomez",fullName:"Hector Gomez-Gomez"},{id:"194006",title:"Prof.",name:"Chung-Yen Oliver",middleName:null,surname:"Chen",slug:"chung-yen-oliver-chen",fullName:"Chung-Yen Oliver Chen"}]}],mostDownloadedChaptersLast30Days:[{id:"55500",title:"Interpretation of Mass Spectra",slug:"interpretation-of-mass-spectra",totalDownloads:12377,totalCrossrefCites:11,totalDimensionsCites:24,abstract:"The chapter includes an introduction to the main ionisation techniques in mass spectrometry and the way the resulting fragments can be analysed. First, the fundamental notions of mass spectrometry are explained, so that the reader can easily cover this chapter (graphs, main pick, molecular ion, illogical pick, nitrogen rule, etc.). Isotopic percentage and nominal mass calculation are also explained along with fragmentation mechanism. A paragraph emphasises the ionisation energy issues, the basics of ionisation voltage, the developing potential and the energy balance. A frame time of the main theoretical milestones in both theory and experimental mass spectrometry is highlighted here. In the second part of the chapter, the molecular fragmentation for alkanes, iso-alkanes, cycloalkanes, halogen, alcohols, phenols, ethers, carbonyl compounds, carboxylic acids and functional derivatives, nitrogen compounds (amines, nitro compounds), sulphur compounds, heterocycles and biomolecules (amino acids, steroids, triglycerides) is explained. Fragmentation schemes are followed by the simplified spectra, which help the understanding of such complex phenomena. At the end of the chapter, acquisition of mass spectrum is discussed. The chapter presented here is an introduction to mass spectrometry, which, we think, helps the understanding of the mechanism of fragmentation corroborating spectral data and molecular structures.",book:{id:"5735",slug:"mass-spectrometry",title:"Mass Spectrometry",fullTitle:"Mass Spectrometry"},signatures:"Teodor Octavian Nicolescu",authors:[{id:"196775",title:"Dr.",name:"Teodor Octavian",middleName:"Octavian",surname:"Nicolescu",slug:"teodor-octavian-nicolescu",fullName:"Teodor Octavian Nicolescu"}]},{id:"57909",title:"Validation of Analytical Methods",slug:"validation-of-analytical-methods",totalDownloads:6879,totalCrossrefCites:13,totalDimensionsCites:20,abstract:"Method validation is a key element in the establishment of reference methods and within the assessment of a laboratory’s competence in generating dependable analytical records. Validation has been placed within the context of the procedure, generating chemical data. Analytical method validation, thinking about the maximum relevant processes for checking the best parameters of analytical methods, using numerous relevant overall performance indicators inclusive of selectivity, specificity, accuracy, precision, linearity, range, limit of detection (LOD), limit of quantification (LOQ), ruggedness, and robustness are severely discussed in an effort to prevent their misguided utilization and ensure scientific correctness and consistency among publications.",book:{id:"6379",slug:"calibration-and-validation-of-analytical-methods-a-sampling-of-current-approaches",title:"Calibration and Validation of Analytical Methods",fullTitle:"Calibration and Validation of Analytical Methods - A Sampling of Current Approaches"},signatures:"Tentu Nageswara Rao",authors:[{id:"220824",title:"Dr.",name:"Tentu",middleName:null,surname:"Nageswara Rao",slug:"tentu-nageswara-rao",fullName:"Tentu Nageswara Rao"}]},{id:"55440",title:"Solubility Products and Solubility Concepts",slug:"solubility-products-and-solubility-concepts",totalDownloads:3051,totalCrossrefCites:6,totalDimensionsCites:7,abstract:"The chapter refers to a general concept of solubility product Ksp of sparingly soluble hydroxides and different salts and calculation of solubility of some hydroxides, oxides, and different salts in aqueous media. A (criticized) conventional approach, based on stoichiometry of a reaction notation and the solubility product of a precipitate, is compared with the unconventional/correct approach based on charge and concentration balances and a detailed physicochemical knowledge on the system considered, and calculations realized according to generalized approach to electrolytic systems (GATES) principles. An indisputable advantage of the latter approach is proved in simulation of static or dynamic, two-phase nonredox or redox systems.",book:{id:"5891",slug:"descriptive-inorganic-chemistry-researches-of-metal-compounds",title:"Descriptive Inorganic Chemistry Researches of Metal Compounds",fullTitle:"Descriptive Inorganic Chemistry Researches of Metal Compounds"},signatures:"Anna Maria Michałowska-Kaczmarczyk, Aneta Spórna-Kucab and\nTadeusz Michałowski",authors:[{id:"35273",title:"Prof.",name:"Tadeusz",middleName:null,surname:"Michalowski",slug:"tadeusz-michalowski",fullName:"Tadeusz Michalowski"},{id:"203867",title:"Dr.",name:"Anna Maria",middleName:null,surname:"Michałowska-Kaczmarczyk",slug:"anna-maria-michalowska-kaczmarczyk",fullName:"Anna Maria Michałowska-Kaczmarczyk"},{id:"203868",title:"Dr.",name:"Aneta",middleName:null,surname:"Spórna-Kucab",slug:"aneta-sporna-kucab",fullName:"Aneta Spórna-Kucab"}]},{id:"62736",title:"Radioisotope: Applications, Effects, and Occupational Protection",slug:"radioisotope-applications-effects-and-occupational-protection",totalDownloads:4493,totalCrossrefCites:8,totalDimensionsCites:14,abstract:"This chapter presents a brief introduction to radioisotopes, sources and types of radiation, applications, effects, and occupational protection. The natural and artificial sources of radiations are discussed with special reference to natural radioactive decay series and artificial radioisotopes. Applications have played significant role in improving the quality of human life. The application of radioisotopes in tracing, radiography, food preservation and sterilization, eradication of insects and pests, medical diagnosis and therapy, and new variety of crops in agricultural field is briefly described. Radiation interacts with matter to produce excitation and ionization of an atom or molecule; as a result physical and biological effects are produced. These effects and mechanisms are discussed. The dosimetric quantities used in radiological protection are described. Radiological protections and the control of occupational and medical exposures are briefly described.",book:{id:"5903",slug:"principles-and-applications-in-nuclear-engineering-radiation-effects-thermal-hydraulics-radionuclide-migration-in-the-environment",title:"Principles and Applications in Nuclear Engineering",fullTitle:"Principles and Applications in Nuclear Engineering - Radiation Effects, Thermal Hydraulics, Radionuclide Migration in the Environment"},signatures:"Sannappa Jadiyappa",authors:[{id:"239626",title:"Dr.",name:null,middleName:null,surname:"Sannappa J.",slug:"sannappa-j.",fullName:"Sannappa J."}]},{id:"58596",title:"Linearity of Calibration Curves for Analytical Methods: A Review of Criteria for Assessment of Method Reliability",slug:"linearity-of-calibration-curves-for-analytical-methods-a-review-of-criteria-for-assessment-of-method",totalDownloads:7983,totalCrossrefCites:19,totalDimensionsCites:42,abstract:"Calibration curve is a regression model used to predict the unknown concentrations of analytes of interest based on the response of the instrument to the known standards. Some statistical analyses are required to choose the best model fitting to the experimental data and also evaluate the linearity and homoscedasticity of the calibration curve. Using an internal standard corrects for the loss of analyte during sample preparation and analysis provided that it is selected appropriately. After the best regression model is selected, the analytical method needs to be validated using quality control (QC) samples prepared and stored in the same temperature as intended for the study samples. Most of the international guidelines require that the parameters, including linearity, specificity, selectivity, accuracy, precision, lower limit of quantification (LLOQ), matrix effect and stability, be assessed during validation. Despite the highly regulated area, some challenges still exist regarding the validation of some analytical methods including methods when no analyte-free matrix is available.",book:{id:"6379",slug:"calibration-and-validation-of-analytical-methods-a-sampling-of-current-approaches",title:"Calibration and Validation of Analytical Methods",fullTitle:"Calibration and Validation of Analytical Methods - A Sampling of Current Approaches"},signatures:"Seyed Mojtaba Moosavi and Sussan Ghassabian",authors:[{id:"216099",title:"Dr.",name:"Sussan",middleName:null,surname:"Ghassabian",slug:"sussan-ghassabian",fullName:"Sussan Ghassabian"},{id:"216101",title:"Mr.",name:"Seyed Mojtaba",middleName:null,surname:"Moosavi",slug:"seyed-mojtaba-moosavi",fullName:"Seyed Mojtaba Moosavi"}]}],onlineFirstChaptersFilter:{topicId:"8",limit:6,offset:0},onlineFirstChaptersCollection:[{id:"82385",title:"Cyclodextrins as Bricks for Tuning Polymer Properties",slug:"cyclodextrins-as-bricks-for-tuning-polymer-properties",totalDownloads:1,totalDimensionsCites:0,doi:"10.5772/intechopen.105688",abstract:"Cyclodextrins are natural cyclic oligosaccharides with a cone shape delimiting a hydrophobic cavity. The rims of cyclodextrins can be functionalized in order to improve their properties. Based on this, cyclodextrins can be linked to polymer chains, which further allows the tuning of the polymer properties. This review describes the methods of polymer functionalization with cyclodextrins and highlights the changes in the physicochemical properties of these materials. This chapter is focused on polymers in solution and in gel states. Cyclodextrin-based polymers are evaluated by various physicochemical methods, such as rheology, calorimetry, and spectroscopy (electron paramagnetic resonance, fluorescence, nuclear magnetic resonance (NMR), Fourier transform infrared (FT-IR), etc.). Both natural and synthetic polymers are considered in this chapter.",book:{id:"11901",title:"Cyclodextrins - New Perspectives",coverURL:"https://cdn.intechopen.com/books/images_new/11901.jpg"},signatures:"Ludmila Aricov, Anca Ruxandra Leontieș, Iulia Matei and Gabriela Ioniță"},{id:"82358",title:"Water Defluoridation Methods Applied in Rural Areas over the World",slug:"water-defluoridation-methods-applied-in-rural-areas-over-the-world",totalDownloads:14,totalDimensionsCites:0,doi:"10.5772/intechopen.105102",abstract:"Overexposure to fluoride (F) through drinking water is the most widespread water problem in the world, but it has now exacerbated due to rapid population growth rates, adverse climatic changes, and increasing levels of water scarcity. Thus, despite the large amounts of data, which has accrued on mitigation methods of high F is still the primary impediment to drinking water programs among many developing nations. The current review chapter on F mitigation techniques applied world-over is aimed at providing a succinct overview of water defluoridation techniques and strategies being used to combat the impact of human F overexposure. It represents a starting point to understand the prospects of reducing the global F impact. It is anticipated that this work will lay a strong foundation for this and also inform strategies for safeguarding public health and the environment from F pollution.",book:{id:"11209",title:"Fluoride",coverURL:"https://cdn.intechopen.com/books/images_new/11209.jpg"},signatures:"Enos Wamalwa Wambu, Franco Frau, Revocatus Machunda, Lilliane Pasape, Stephen S. Barasa and Giorgio Ghiglieri"},{id:"82221",title:"Solvent Catalysis in the Sensitizer-Mediator Redox Kinetics",slug:"solvent-catalysis-in-the-sensitizer-mediator-redox-kinetics",totalDownloads:2,totalDimensionsCites:0,doi:"10.5772/intechopen.105393",abstract:"The sensitizer-mediator redox reaction is a vital component of the dye-sensitized solar cells (DSSCs). The efficiency and stability of dye-sensitized solar cells are aided by the kinetics of this redox process. Several reaction parameters influence the kinetics of a reaction, and if those parameters are controlled, the rate of the process and its results can be controlled. One of the most important aspects of the sensitizer-mediator interaction is the reaction medium. Aqueous DSSCs are unquestionably a good replacement when it comes to taking a green approach to avoiding toxic, flammable, and volatile organic solvents and their mixtures, which are commonly used in DSSCs and are known to harm the environment while also reducing the lifetime and stability of the DSSCs. The catalytic role of a small volume fraction of organic solvent in the aqueous electron transfer kinetics of a few putative sensitizer-mediator reactions is discussed in this chapter. In binary solvent media including dilute tertiary butyl alcohol (TBA)-water and dilute 1,4-dioxane-water, the reduction of dicyanobis(2,2′-dipyridyl)iron(III) and dicyanobis(1,10-phenanthroline)iron(III) was investigated. The reactions were carried out in a 10% TBA or dioxane to water media with a volume-volume fraction of both solvents using iodide as a reducing agent. The effect of several parameters on the rate constant was also calculated and analyzed.",book:{id:"11217",title:"Recent Advances in Chemical Kinetics",coverURL:"https://cdn.intechopen.com/books/images_new/11217.jpg"},signatures:"Rozina Khattak"},{id:"82282",title:"Pyridine Nucleus as a Directing Group for Metal-Based C–H Bond Activation",slug:"pyridine-nucleus-as-a-directing-group-for-metal-based-c-h-bond-activation",totalDownloads:6,totalDimensionsCites:0,doi:"10.5772/intechopen.105544",abstract:"Carbon-hydrogen (C–H) bond activation involves a methodology for the construction of carbon-X (C–X) bonds where X can be carbon (C), oxygen (O), or the nitrogen (N), allowing the formation of C–C, C–O, or C–N bonds. Among them, the construction of the C–C bond within the aromatic moiety has remained a bottleneck because the abundance of C–H bonds in aromatic molecules possesses almost similar bond dissociation energies comparable to the C–C bond allowing leading to the poor reactivity and selectivity. Secondly, C–H bonds possess low polarity and thus confer them inertness. Considering this, directing group strategy came into existence, where the coordination ability of the heteroatoms such as O and N atoms within the ring was utilized for the direction of the reaction. The use of the heteroatom for the regioselective C–H bond activation is quite advantageous that could be explored immensely for their functionalization. In this chapter, we have congregated the information and put forth the evidence of C–H activation leading to the C–C bond formation in pyridine and pyridine-containing entities.",book:{id:"11562",title:"Chemistry with Pyridine Derivatives",coverURL:"https://cdn.intechopen.com/books/images_new/11562.jpg"},signatures:"Purohit Priyank, Joshi Gaurav and Aggarwal Meenu"},{id:"82236",title:"Alternatives to Soluble Phosphorus Fertilizers in Indian Context",slug:"alternatives-to-soluble-phosphorus-fertilizers-in-indian-context",totalDownloads:7,totalDimensionsCites:0,doi:"10.5772/intechopen.105561",abstract:"Phosphorus is one of the primary nutrients required in crop production. Rock phosphate is the raw material required for the manufacturing of soluble phosphorus fertilizers, which is nonrenewable in nature and expected to last for 50–400 years. The restriction of resources to few geographical locations makes its supply more vulnerable. In India, 90% of the rock phosphate for fertilizer manufacturing is imported. However, the low quality of rock phosphate deposits available in India can be utilized with certain modifications in the form of addition of phosphate-solubilizing bacteria, addition of gypsum, and in the form of phospho-enriched compost. Agriculture, livestock, urban and industrial waste can also prove to be a source of phosphorus through crystallization of struvite. There are encouraging results of struvite compared with soluble phosphorus fertilizers. This will reduce the import dependency in India as well as encourage the Atmanirbhar initiative in phosphorus fertilizer.",book:{id:"11906",title:"Phosphate Minerals",coverURL:"https://cdn.intechopen.com/books/images_new/11906.jpg"},signatures:"Alok Singh Jayara, Rajeew Kumar, Priyanka Pandey, Manoj Kumar Bhatt, Sharad Pandey and Roshan Lal Meena"},{id:"82251",title:"Potassium Persulfate as an Eco-Friendly Oxidant for Oxidative Transformations",slug:"potassium-persulfate-as-an-eco-friendly-oxidant-for-oxidative-transformations",totalDownloads:14,totalDimensionsCites:0,doi:"10.5772/intechopen.104715",abstract:"The formation of carbon-carbon/carbon-heteroatom bonds by oxidative transformations is a hotly debated topic in chemistry. K2S2O8 has emerged as a cost-effective inorganic oxidant for a wide range of oxidative reactions in this setting. This book chapter covers oxidative reactions facilitated by K2S2O8 in the absence of a metal catalyst in detail. Organic chemists may find this book chapter valuable in formulating the mechanistic pathways involving the sulphate radical anion, as well as in the quick and environmentally friendly synthesis of novel chemical species.",book:{id:"11211",title:"Green Chemistry - New Perspectives",coverURL:"https://cdn.intechopen.com/books/images_new/11211.jpg"},signatures:"Bilal Ahmad Mir and Suresh Rajamanickam"}],onlineFirstChaptersTotal:77},preDownload:{success:null,errors:{}},subscriptionForm:{success:null,errors:{}},aboutIntechopen:{},privacyPolicy:{},peerReviewing:{},howOpenAccessPublishingWithIntechopenWorks:{},sponsorshipBooks:{sponsorshipBooks:[],offset:0,limit:8,total:null},allSeries:{pteSeriesList:[{id:"14",title:"Artificial Intelligence",numberOfPublishedBooks:9,numberOfPublishedChapters:89,numberOfOpenTopics:6,numberOfUpcomingTopics:0,issn:"2633-1403",doi:"10.5772/intechopen.79920",isOpenForSubmission:!0},{id:"7",title:"Biomedical Engineering",numberOfPublishedBooks:12,numberOfPublishedChapters:104,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2631-5343",doi:"10.5772/intechopen.71985",isOpenForSubmission:!0}],lsSeriesList:[{id:"11",title:"Biochemistry",numberOfPublishedBooks:32,numberOfPublishedChapters:318,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2632-0983",doi:"10.5772/intechopen.72877",isOpenForSubmission:!0},{id:"25",title:"Environmental Sciences",numberOfPublishedBooks:1,numberOfPublishedChapters:12,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2754-6713",doi:"10.5772/intechopen.100362",isOpenForSubmission:!0},{id:"10",title:"Physiology",numberOfPublishedBooks:11,numberOfPublishedChapters:141,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2631-8261",doi:"10.5772/intechopen.72796",isOpenForSubmission:!0}],hsSeriesList:[{id:"3",title:"Dentistry",numberOfPublishedBooks:8,numberOfPublishedChapters:129,numberOfOpenTopics:2,numberOfUpcomingTopics:0,issn:"2631-6218",doi:"10.5772/intechopen.71199",isOpenForSubmission:!0},{id:"6",title:"Infectious Diseases",numberOfPublishedBooks:13,numberOfPublishedChapters:113,numberOfOpenTopics:3,numberOfUpcomingTopics:1,issn:"2631-6188",doi:"10.5772/intechopen.71852",isOpenForSubmission:!0},{id:"13",title:"Veterinary Medicine and Science",numberOfPublishedBooks:11,numberOfPublishedChapters:106,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2632-0517",doi:"10.5772/intechopen.73681",isOpenForSubmission:!0}],sshSeriesList:[{id:"22",title:"Business, Management and Economics",numberOfPublishedBooks:1,numberOfPublishedChapters:19,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2753-894X",doi:"10.5772/intechopen.100359",isOpenForSubmission:!0},{id:"23",title:"Education and Human Development",numberOfPublishedBooks:0,numberOfPublishedChapters:5,numberOfOpenTopics:1,numberOfUpcomingTopics:1,issn:null,doi:"10.5772/intechopen.100360",isOpenForSubmission:!0},{id:"24",title:"Sustainable Development",numberOfPublishedBooks:0,numberOfPublishedChapters:15,numberOfOpenTopics:5,numberOfUpcomingTopics:0,issn:null,doi:"10.5772/intechopen.100361",isOpenForSubmission:!0}],testimonialsList:[{id:"6",text:"It is great to work with the IntechOpen to produce a worthwhile collection of research that also becomes a great educational resource and guide for future research endeavors.",author:{id:"259298",name:"Edward",surname:"Narayan",institutionString:null,profilePictureURL:"https://mts.intechopen.com/storage/users/259298/images/system/259298.jpeg",slug:"edward-narayan",institution:{id:"3",name:"University of Queensland",country:{id:null,name:"Australia"}}}},{id:"13",text:"The collaboration with and support of the technical staff of IntechOpen is fantastic. The whole process of submitting an article and editing of the submitted article goes extremely smooth and fast, the number of reads and downloads of chapters is high, and the contributions are also frequently cited.",author:{id:"55578",name:"Antonio",surname:"Jurado-Navas",institutionString:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRisIQAS/Profile_Picture_1626166543950",slug:"antonio-jurado-navas",institution:{id:"720",name:"University of Malaga",country:{id:null,name:"Spain"}}}}]},series:{item:{id:"14",title:"Artificial Intelligence",doi:"10.5772/intechopen.79920",issn:"2633-1403",scope:"Artificial Intelligence (AI) is a rapidly developing multidisciplinary research area that aims to solve increasingly complex problems. In today's highly integrated world, AI promises to become a robust and powerful means for obtaining solutions to previously unsolvable problems. This Series is intended for researchers and students alike interested in this fascinating field and its many applications.",coverUrl:"https://cdn.intechopen.com/series/covers/14.jpg",latestPublicationDate:"June 11th, 2022",hasOnlineFirst:!0,numberOfPublishedBooks:9,editor:{id:"218714",title:"Prof.",name:"Andries",middleName:null,surname:"Engelbrecht",slug:"andries-engelbrecht",fullName:"Andries Engelbrecht",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRNR8QAO/Profile_Picture_1622640468300",biography:"Andries Engelbrecht received the Masters and PhD degrees in Computer Science from the University of Stellenbosch, South Africa, in 1994 and 1999 respectively. He is currently appointed as the Voigt Chair in Data Science in the Department of Industrial Engineering, with a joint appointment as Professor in the Computer Science Division, Stellenbosch University. Prior to his appointment at Stellenbosch University, he has been at the University of Pretoria, Department of Computer Science (1998-2018), where he was appointed as South Africa Research Chair in Artifical Intelligence (2007-2018), the head of the Department of Computer Science (2008-2017), and Director of the Institute for Big Data and Data Science (2017-2018). In addition to a number of research articles, he has written two books, Computational Intelligence: An Introduction and Fundamentals of Computational Swarm Intelligence.",institutionString:null,institution:{name:"Stellenbosch University",institutionURL:null,country:{name:"South Africa"}}},editorTwo:null,editorThree:null},subseries:{paginationCount:6,paginationItems:[{id:"22",title:"Applied Intelligence",coverUrl:"https://cdn.intechopen.com/series_topics/covers/22.jpg",isOpenForSubmission:!0,editor:{id:"27170",title:"Prof.",name:"Carlos",middleName:"M.",surname:"Travieso-Gonzalez",slug:"carlos-travieso-gonzalez",fullName:"Carlos Travieso-Gonzalez",profilePictureURL:"https://mts.intechopen.com/storage/users/27170/images/system/27170.jpeg",biography:"Carlos M. Travieso-González received his MSc degree in Telecommunication Engineering at Polytechnic University of Catalonia (UPC), Spain in 1997, and his Ph.D. degree in 2002 at the University of Las Palmas de Gran Canaria (ULPGC-Spain). He is a full professor of signal processing and pattern recognition and is head of the Signals and Communications Department at ULPGC, teaching from 2001 on subjects on signal processing and learning theory. His research lines are biometrics, biomedical signals and images, data mining, classification system, signal and image processing, machine learning, and environmental intelligence. He has researched in 52 international and Spanish research projects, some of them as head researcher. He is co-author of 4 books, co-editor of 27 proceedings books, guest editor for 8 JCR-ISI international journals, and up to 24 book chapters. He has over 450 papers published in international journals and conferences (81 of them indexed on JCR – ISI - Web of Science). He has published seven patents in the Spanish Patent and Trademark Office. He has been a supervisor on 8 Ph.D. theses (11 more are under supervision), and 130 master theses. He is the founder of The IEEE IWOBI conference series and the president of its Steering Committee, as well as the founder of both the InnoEducaTIC and APPIS conference series. He is an evaluator of project proposals for the European Union (H2020), Medical Research Council (MRC, UK), Spanish Government (ANECA, Spain), Research National Agency (ANR, France), DAAD (Germany), Argentinian Government, and the Colombian Institutions. He has been a reviewer in different indexed international journals (<70) and conferences (<250) since 2001. He has been a member of the IASTED Technical Committee on Image Processing from 2007 and a member of the IASTED Technical Committee on Artificial Intelligence and Expert Systems from 2011. \n\nHe has held the general chair position for the following: ACM-APPIS (2020, 2021), IEEE-IWOBI (2019, 2020 and 2020), A PPIS (2018, 2019), IEEE-IWOBI (2014, 2015, 2017, 2018), InnoEducaTIC (2014, 2017), IEEE-INES (2013), NoLISP (2011), JRBP (2012), and IEEE-ICCST (2005)\n\nHe is an associate editor of the Computational Intelligence and Neuroscience Journal (Hindawi – Q2 JCR-ISI). He was vice dean from 2004 to 2010 in the Higher Technical School of Telecommunication Engineers at ULPGC and the vice dean of Graduate and Postgraduate Studies from March 2013 to November 2017. He won the “Catedra Telefonica” Awards in Modality of Knowledge Transfer, 2017, 2018, and 2019 editions, and awards in Modality of COVID Research in 2020.\n\nPublic References:\nResearcher ID http://www.researcherid.com/rid/N-5967-2014\nORCID https://orcid.org/0000-0002-4621-2768 \nScopus Author ID https://www.scopus.com/authid/detail.uri?authorId=6602376272\nScholar Google https://scholar.google.es/citations?user=G1ks9nIAAAAJ&hl=en \nResearchGate https://www.researchgate.net/profile/Carlos_Travieso",institutionString:null,institution:{name:"University of Las Palmas de Gran Canaria",institutionURL:null,country:{name:"Spain"}}},editorTwo:null,editorThree:null},{id:"23",title:"Computational Neuroscience",coverUrl:"https://cdn.intechopen.com/series_topics/covers/23.jpg",isOpenForSubmission:!0,editor:{id:"14004",title:"Dr.",name:"Magnus",middleName:null,surname:"Johnsson",slug:"magnus-johnsson",fullName:"Magnus Johnsson",profilePictureURL:"https://mts.intechopen.com/storage/users/14004/images/system/14004.png",biography:"Dr Magnus Johnsson is a cross-disciplinary scientist, lecturer, scientific editor and AI/machine learning consultant from Sweden. \n\nHe is currently at Malmö University in Sweden, but also held positions at Lund University in Sweden and at Moscow Engineering Physics Institute. \nHe holds editorial positions at several international scientific journals and has served as a scientific editor for books and special journal issues. \nHis research interests are wide and include, but are not limited to, autonomous systems, computer modeling, artificial neural networks, artificial intelligence, cognitive neuroscience, cognitive robotics, cognitive architectures, cognitive aids and the philosophy of mind. \n\nDr. Johnsson has experience from working in the industry and he has a keen interest in the application of neural networks and artificial intelligence to fields like industry, finance, and medicine. \n\nWeb page: www.magnusjohnsson.se",institutionString:null,institution:{name:"Malmö University",institutionURL:null,country:{name:"Sweden"}}},editorTwo:null,editorThree:null},{id:"24",title:"Computer Vision",coverUrl:"https://cdn.intechopen.com/series_topics/covers/24.jpg",isOpenForSubmission:!0,editor:{id:"294154",title:"Prof.",name:"George",middleName:null,surname:"Papakostas",slug:"george-papakostas",fullName:"George Papakostas",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002hYaGbQAK/Profile_Picture_1624519712088",biography:"George A. 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He has (co)authored more than 150 publications in indexed journals, international conferences and book chapters, 1 book (in Greek), 3 edited books, and 5 journal special issues. His publications have more than 2100 citations with h-index 27 (GoogleScholar). His research interests include computer/machine vision, machine learning, pattern recognition, computational intelligence. \nDr. Papakostas served as a reviewer in numerous journals, as a program\ncommittee member in international conferences and he is a member of the IAENG, MIR Labs, EUCogIII, INSTICC and the Technical Chamber of Greece (TEE).",institutionString:null,institution:{name:"International Hellenic University",institutionURL:null,country:{name:"Greece"}}},editorTwo:null,editorThree:null},{id:"25",title:"Evolutionary Computation",coverUrl:"https://cdn.intechopen.com/series_topics/covers/25.jpg",isOpenForSubmission:!0,editor:{id:"136112",title:"Dr.",name:"Sebastian",middleName:null,surname:"Ventura Soto",slug:"sebastian-ventura-soto",fullName:"Sebastian Ventura Soto",profilePictureURL:"https://mts.intechopen.com/storage/users/136112/images/system/136112.png",biography:"Sebastian Ventura is a Spanish researcher, a full professor with the Department of Computer Science and Numerical Analysis, University of Córdoba. Dr Ventura also holds the positions of Affiliated Professor at Virginia Commonwealth University (Richmond, USA) and Distinguished Adjunct Professor at King Abdulaziz University (Jeddah, Saudi Arabia). Additionally, he is deputy director of the Andalusian Research Institute in Data Science and Computational Intelligence (DaSCI) and heads the Knowledge Discovery and Intelligent Systems Research Laboratory. He has published more than ten books and over 300 articles in journals and scientific conferences. Currently, his work has received over 18,000 citations according to Google Scholar, including more than 2200 citations in 2020. In the last five years, he has published more than 60 papers in international journals indexed in the JCR (around 70% of them belonging to first quartile journals) and he has edited some Springer books “Supervised Descriptive Pattern Mining” (2018), “Multiple Instance Learning - Foundations and Algorithms” (2016), and “Pattern Mining with Evolutionary Algorithms” (2016). He has also been involved in more than 20 research projects supported by the Spanish and Andalusian governments and the European Union. He currently belongs to the editorial board of PeerJ Computer Science, Information Fusion and Engineering Applications of Artificial Intelligence journals, being also associate editor of Applied Computational Intelligence and Soft Computing and IEEE Transactions on Cybernetics. Finally, he is editor-in-chief of Progress in Artificial Intelligence. He is a Senior Member of the IEEE Computer, the IEEE Computational Intelligence, and the IEEE Systems, Man, and Cybernetics Societies, and the Association of Computing Machinery (ACM). Finally, his main research interests include data science, computational intelligence, and their applications.",institutionString:null,institution:{name:"University of Córdoba",institutionURL:null,country:{name:"Spain"}}},editorTwo:null,editorThree:null},{id:"26",title:"Machine Learning and Data Mining",coverUrl:"https://cdn.intechopen.com/series_topics/covers/26.jpg",isOpenForSubmission:!0,editor:{id:"24555",title:"Dr.",name:"Marco Antonio",middleName:null,surname:"Aceves Fernandez",slug:"marco-antonio-aceves-fernandez",fullName:"Marco Antonio Aceves Fernandez",profilePictureURL:"https://mts.intechopen.com/storage/users/24555/images/system/24555.jpg",biography:"Dr. Marco Antonio Aceves Fernandez obtained his B.Sc. (Eng.) in Telematics from the Universidad de Colima, Mexico. He obtained both his M.Sc. and Ph.D. from the University of Liverpool, England, in the field of Intelligent Systems. He is a full professor at the Universidad Autonoma de Queretaro, Mexico, and a member of the National System of Researchers (SNI) since 2009. Dr. Aceves Fernandez has published more than 80 research papers as well as a number of book chapters and congress papers. He has contributed in more than 20 funded research projects, both academic and industrial, in the area of artificial intelligence, ranging from environmental, biomedical, automotive, aviation, consumer, and robotics to other applications. He is also a honorary president at the National Association of Embedded Systems (AMESE), a senior member of the IEEE, and a board member of many institutions. 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He is currently a principal researcher in data analytics and optimisation at TECNALIA (Spain), a visiting fellow at the Basque Center for Applied Mathematics (BCAM) and a part-time lecturer at the University of the Basque Country (UPV/EHU). His research interests gravitate on the use of descriptive, prescriptive and predictive algorithms for data mining and optimization in a diverse range of application fields such as Energy, Transport, Telecommunications, Health and Industry, among others. In these fields he has published more than 240 articles, co-supervised 8 Ph.D. theses, edited 6 books, coauthored 7 patents and participated/led more than 40 research projects. 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He has both an MS and Ph.D. in Biomedical Engineering. He was previously a research scientist at the University of California Los Angeles (UCLA) and visiting professor and researcher at the University of North Dakota. He is currently working in artificial intelligence and its applications in medical signal processing. In addition, he is using digital signal processing in medical imaging and speech processing. Dr. Asadpour has developed brain-computer interfacing algorithms and has published books, book chapters, and several journal and conference papers in this field and other areas of intelligent signal processing. He has also designed medical devices, including a laser Doppler monitoring system.",institutionString:"Kaiser Permanente Southern California",institution:null},{id:"169608",title:"Prof.",name:"Marian",middleName:null,surname:"Găiceanu",slug:"marian-gaiceanu",fullName:"Marian Găiceanu",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/169608/images/system/169608.png",biography:"Prof. Dr. Marian Gaiceanu graduated from the Naval and Electrical Engineering Faculty, Dunarea de Jos University of Galati, Romania, in 1997. He received a Ph.D. (Magna Cum Laude) in Electrical Engineering in 2002. Since 2017, Dr. Gaiceanu has been a Ph.D. supervisor for students in Electrical Engineering. He has been employed at Dunarea de Jos University of Galati since 1996, where he is currently a professor. Dr. Gaiceanu is a member of the National Council for Attesting Titles, Diplomas and Certificates, an expert of the Executive Agency for Higher Education, Research Funding, and a member of the Senate of the Dunarea de Jos University of Galati. He has been the head of the Integrated Energy Conversion Systems and Advanced Control of Complex Processes Research Center, Romania, since 2016. He has conducted several projects in power converter systems for electrical drives, power quality, PEM and SOFC fuel cell power converters for utilities, electric vehicles, and marine applications with the Department of Regulation and Control, SIEI S.pA. (2002–2004) and the Polytechnic University of Turin, Italy (2002–2004, 2006–2007). He is a member of the Institute of Electrical and Electronics Engineers (IEEE) and cofounder-member of the IEEE Power Electronics Romanian Chapter. He is a guest editor at Energies and an academic book editor for IntechOpen. He is also a member of the editorial boards of the Journal of Electrical Engineering, Electronics, Control and Computer Science and Sustainability. Dr. Gaiceanu has been General Chairman of the IEEE International Symposium on Electrical and Electronics Engineering in the last six editions.",institutionString:'"Dunarea de Jos" University of Galati',institution:{name:'"Dunarea de Jos" University of Galati',country:{name:"Romania"}}},{id:"4519",title:"Prof.",name:"Jaydip",middleName:null,surname:"Sen",slug:"jaydip-sen",fullName:"Jaydip Sen",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/4519/images/system/4519.jpeg",biography:"Jaydip Sen is associated with Praxis Business School, Kolkata, India, as a professor in the Department of Data Science. His research areas include security and privacy issues in computing and communication, intrusion detection systems, machine learning, deep learning, and artificial intelligence in the financial domain. He has more than 200 publications in reputed international journals, refereed conference proceedings, and 20 book chapters in books published by internationally renowned publishing houses, such as Springer, CRC press, IGI Global, etc. Currently, he is serving on the editorial board of the prestigious journal Frontiers in Communications and Networks and in the technical program committees of a number of high-ranked international conferences organized by the IEEE, USA, and the ACM, USA. He has been listed among the top 2% of scientists in the world for the last three consecutive years, 2019 to 2021 as per studies conducted by the Stanford University, USA.",institutionString:"Praxis Business School",institution:null},{id:"320071",title:"Dr.",name:"Sidra",middleName:null,surname:"Mehtab",slug:"sidra-mehtab",fullName:"Sidra Mehtab",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y00002v6KHoQAM/Profile_Picture_1584512086360",biography:"Sidra Mehtab has completed her BS with honors in Physics from Calcutta University, India in 2018. She has done MS in Data Science and Analytics from Maulana Abul Kalam Azad University of Technology (MAKAUT), Kolkata, India in 2020. Her research areas include Econometrics, Time Series Analysis, Machine Learning, Deep Learning, Artificial Intelligence, and Computer and Network Security with a particular focus on Cyber Security Analytics. Ms. Mehtab has published seven papers in international conferences and one of her papers has been accepted for publication in a reputable international journal. She has won the best paper awards in two prestigious international conferences – BAICONF 2019, and ICADCML 2021, organized in the Indian Institute of Management, Bangalore, India in December 2019, and SOA University, Bhubaneswar, India in January 2021. Besides, Ms. Mehtab has also published two book chapters in two books. Seven of her book chapters will be published in a volume shortly in 2021 by Cambridge Scholars’ Press, UK. Currently, she is working as the joint editor of two edited volumes on Time Series Analysis and Forecasting to be published in the first half of 2021 by an international house. Currently, she is working as a Data Scientist with an MNC in Delhi, India.",institutionString:"NSHM College of Management and Technology",institution:null},{id:"226240",title:"Dr.",name:"Andri Irfan",middleName:null,surname:"Rifai",slug:"andri-irfan-rifai",fullName:"Andri Irfan Rifai",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/226240/images/7412_n.jpg",biography:"Andri IRFAN is a Senior Lecturer of Civil Engineering and Planning. He completed the PhD at the Universitas Indonesia & Universidade do Minho with Sandwich Program Scholarship from the Directorate General of Higher Education and LPDP scholarship. He has been teaching for more than 19 years and much active to applied his knowledge in the project construction in Indonesia. His research interest ranges from pavement management system to advanced data mining techniques for transportation engineering. He has published more than 50 papers in journals and 2 books.",institutionString:null,institution:{name:"Universitas Internasional Batam",country:{name:"Indonesia"}}},{id:"314576",title:"Dr.",name:"Ibai",middleName:null,surname:"Laña",slug:"ibai-lana",fullName:"Ibai Laña",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/314576/images/system/314576.jpg",biography:"Dr. Ibai Laña works at TECNALIA as a data analyst. He received his Ph.D. in Artificial Intelligence from the University of the Basque Country (UPV/EHU), Spain, in 2018. He is currently a senior researcher at TECNALIA. His research interests fall within the intersection of intelligent transportation systems, machine learning, traffic data analysis, and data science. He has dealt with urban traffic forecasting problems, applying machine learning models and evolutionary algorithms. He has experience in origin-destination matrix estimation or point of interest and trajectory detection. Working with large volumes of data has given him a good command of big data processing tools and NoSQL databases. He has also been a visiting scholar at the Knowledge Engineering and Discovery Research Institute, Auckland University of Technology.",institutionString:"TECNALIA Research & Innovation",institution:{name:"Tecnalia",country:{name:"Spain"}}},{id:"314575",title:"Dr.",name:"Jesus",middleName:null,surname:"L. Lobo",slug:"jesus-l.-lobo",fullName:"Jesus L. Lobo",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/314575/images/system/314575.png",biography:"Dr. Jesús López is currently based in Bilbao (Spain) working at TECNALIA as Artificial Intelligence Research Scientist. In most cases, a project idea or a new research line needs to be investigated to see if it is good enough to take into production or to focus on it. That is exactly what he does, diving into Machine Learning algorithms and technologies to help TECNALIA to decide whether something is great in theory or will actually impact on the product or processes of its projects. So, he is expert at framing experiments, developing hypotheses, and proving whether they’re true or not, in order to investigate fundamental problems with a longer time horizon. He is also able to design and develop PoCs and system prototypes in simulation. He has participated in several national and internacional R&D projects.\n\nAs another relevant part of his everyday research work, he usually publishes his findings in reputed scientific refereed journals and international conferences, occasionally acting as reviewer and Programme Commitee member. Concretely, since 2018 he has published 9 JCR (8 Q1) journal papers, 9 conference papers (e.g. ECML PKDD 2021), and he has co-edited a book. He is also active in popular science writing data science stories for reputed blogs (KDNuggets, TowardsDataScience, Naukas). Besides, he has recently embarked on mentoring programmes as mentor, and has also worked as data science trainer.",institutionString:"TECNALIA Research & Innovation",institution:{name:"Tecnalia",country:{name:"Spain"}}},{id:"103779",title:"Prof.",name:"Yalcin",middleName:null,surname:"Isler",slug:"yalcin-isler",fullName:"Yalcin Isler",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRyQ8QAK/Profile_Picture_1628834958734",biography:"Yalcin Isler (1971 - Burdur / Turkey) received the B.Sc. degree in the Department of Electrical and Electronics Engineering from Anadolu University, Eskisehir, Turkey, in 1993, the M.Sc. degree from the Department of Electronics and Communication Engineering, Suleyman Demirel University, Isparta, Turkey, in 1996, the Ph.D. degree from the Department of Electrical and Electronics Engineering, Dokuz Eylul University, Izmir, Turkey, in 2009, and the Competence of Associate Professorship from the Turkish Interuniversity Council in 2019.\n\nHe was Lecturer at Burdur Vocational School in Suleyman Demirel University (1993-2000, Burdur / Turkey), Software Engineer (2000-2002, Izmir / Turkey), Research Assistant in Bulent Ecevit University (2002-2003, Zonguldak / Turkey), Research Assistant in Dokuz Eylul University (2003-2010, Izmir / Turkey), Assistant Professor at the Department of Electrical and Electronics Engineering in Bulent Ecevit University (2010-2012, Zonguldak / Turkey), Assistant Professor at the Department of Biomedical Engineering in Izmir Katip Celebi University (2012-2019, Izmir / Turkey). He is an Associate Professor at the Department of Biomedical Engineering at Izmir Katip Celebi University, Izmir / Turkey, since 2019. In addition to academics, he has also founded Islerya Medical and Information Technologies Company, Izmir / Turkey, since 2017.\n\nHis main research interests cover biomedical signal processing, pattern recognition, medical device design, programming, and embedded systems. He has many scientific papers and participated in several projects in these study fields. He was an IEEE Student Member (2009-2011) and IEEE Member (2011-2014) and has been IEEE Senior Member since 2014.",institutionString:null,institution:{name:"Izmir Kâtip Çelebi University",country:{name:"Turkey"}}},{id:"339677",title:"Dr.",name:"Mrinmoy",middleName:null,surname:"Roy",slug:"mrinmoy-roy",fullName:"Mrinmoy Roy",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/339677/images/16768_n.jpg",biography:"An accomplished Sales & Marketing professional with 12 years of cross-functional experience in well-known organisations such as CIPLA, LUPIN, GLENMARK, ASTRAZENECA across different segment of Sales & Marketing, International Business, Institutional Business, Product Management, Strategic Marketing of HIV, Oncology, Derma, Respiratory, Anti-Diabetic, Nutraceutical & Stomatological Product Portfolio and Generic as well as Chronic Critical Care Portfolio. A First Class MBA in International Business & Strategic Marketing, B.Pharm, D.Pharm, Google Certified Digital Marketing Professional. Qualified PhD Candidate in Operations and Management with special focus on Artificial Intelligence and Machine Learning adoption, analysis and use in Healthcare, Hospital & Pharma Domain. Seasoned with diverse therapy area of Pharmaceutical Sales & Marketing ranging from generating revenue through generating prescriptions, launching new products, and making them big brands with continuous strategy execution at the Physician and Patients level. Moved from Sales to Marketing and Business Development for 3.5 years in South East Asian Market operating from Manila, Philippines. Came back to India and handled and developed Brands such as Gluconorm, Lupisulin, Supracal, Absolut Woman, Hemozink, Fabiflu (For COVID 19), and many more. In my previous assignment I used to develop and execute strategies on Sales & Marketing, Commercialization & Business Development for Institution and Corporate Hospital Business portfolio of Oncology Therapy Area for AstraZeneca Pharma India Ltd. Being a Research Scholar and Student of ‘Operations Research & Management: Artificial Intelligence’ I published several pioneer research papers and book chapters on the same in Internationally reputed journals and Books indexed in Scopus, Springer and Ei Compendex, Google Scholar etc. Currently, I am launching PGDM Pharmaceutical Management Program in IIHMR Bangalore and spearheading the course curriculum and structure of the same. I am interested in Collaboration for Healthcare Innovation, Pharma AI Innovation, Future trend in Marketing and Management with incubation on Healthcare, Healthcare IT startups, AI-ML Modelling and Healthcare Algorithm based training module development. I am also an affiliated member of the Institute of Management Consultant of India, looking forward to Healthcare, Healthcare IT and Innovation, Pharma and Hospital Management Consulting works.",institutionString:null,institution:{name:"Lovely Professional University",country:{name:"India"}}},{id:"310576",title:"Prof.",name:"Erick Giovani",middleName:null,surname:"Sperandio Nascimento",slug:"erick-giovani-sperandio-nascimento",fullName:"Erick Giovani Sperandio Nascimento",position:null,profilePictureURL:"https://intech-files.s3.amazonaws.com/0033Y00002pDKxDQAW/ProfilePicture%202022-06-20%2019%3A57%3A24.788",biography:"Prof. Erick Sperandio is the Lead Researcher and professor of Artificial Intelligence (AI) at SENAI CIMATEC, Bahia, Brazil, also working with Computational Modeling (CM) and HPC. He holds a PhD in Environmental Engineering in the area of Atmospheric Computational Modeling, a Master in Informatics in the field of Computational Intelligence and Graduated in Computer Science from UFES. He currently coordinates, leads and participates in R&D projects in the areas of AI, computational modeling and supercomputing applied to different areas such as Oil and Gas, Health, Advanced Manufacturing, Renewable Energies and Atmospheric Sciences, advising undergraduate, master's and doctoral students. He is the Lead Researcher at SENAI CIMATEC's Reference Center on Artificial Intelligence. In addition, he is a Certified Instructor and University Ambassador of the NVIDIA Deep Learning Institute (DLI) in the areas of Deep Learning, Computer Vision, Natural Language Processing and Recommender Systems, and Principal Investigator of the NVIDIA/CIMATEC AI Joint Lab, the first in Latin America within the NVIDIA AI Technology Center (NVAITC) worldwide program. He also works as a researcher at the Supercomputing Center for Industrial Innovation (CS2i) and at the SENAI Institute of Innovation for Automation (ISI Automação), both from SENAI CIMATEC. He is a member and vice-coordinator of the Basic Board of Scientific-Technological Advice and Evaluation, in the area of Innovation, of the Foundation for Research Support of the State of Bahia (FAPESB). He serves as Technology Transfer Coordinator and one of the Principal Investigators at the National Applied Research Center in Artificial Intelligence (CPA-IA) of SENAI CIMATEC, focusing on Industry, being one of the six CPA-IA in Brazil approved by MCTI / FAPESP / CGI.br. He also participates as one of the representatives of Brazil in the BRICS Innovation Collaboration Working Group on HPC, ICT and AI. He is the coordinator of the Work Group of the Axis 5 - Workforce and Training - of the Brazilian Strategy for Artificial Intelligence (EBIA), and member of the MCTI/EMBRAPII AI Innovation Network Training Committee. He is the coordinator, by SENAI CIMATEC, of the Artificial Intelligence Reference Network of the State of Bahia (REDE BAH.IA). He leads the working group of experts representing Brazil in the Global Partnership on Artificial Intelligence (GPAI), on the theme \"AI and the Pandemic Response\".",institutionString:"Manufacturing and Technology Integrated Campus – SENAI CIMATEC",institution:null},{id:"1063",title:"Prof.",name:"Constantin",middleName:null,surname:"Volosencu",slug:"constantin-volosencu",fullName:"Constantin Volosencu",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/1063/images/system/1063.png",biography:"Prof. Dr. Constantin Voloşencu graduated as an engineer from\nPolitehnica University of Timișoara, Romania, where he also\nobtained a doctorate degree. He is currently a full professor in\nthe Department of Automation and Applied Informatics at the\nsame university. Dr. Voloşencu is the author of ten books, seven\nbook chapters, and more than 160 papers published in journals\nand conference proceedings. He has also edited twelve books and\nhas twenty-seven patents to his name. He is a manager of research grants, editor in\nchief and member of international journal editorial boards, a former plenary speaker, a member of scientific committees, and chair at international conferences. His\nresearch is in the fields of control systems, control of electric drives, fuzzy control\nsystems, neural network applications, fault detection and diagnosis, sensor network\napplications, monitoring of distributed parameter systems, and power ultrasound\napplications. He has developed automation equipment for machine tools, spooling\nmachines, high-power ultrasound processes, and more.",institutionString:"Polytechnic University of Timişoara",institution:{name:"Polytechnic University of Timişoara",country:{name:"Romania"}}},{id:"221364",title:"Dr.",name:"Eneko",middleName:null,surname:"Osaba",slug:"eneko-osaba",fullName:"Eneko Osaba",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/221364/images/system/221364.jpg",biography:"Dr. Eneko Osaba works at TECNALIA as a senior researcher. He obtained his Ph.D. in Artificial Intelligence in 2015. He has participated in more than twenty-five local and European research projects, and in the publication of more than 130 papers. He has performed several stays at universities in the United Kingdom, Italy, and Malta. Dr. Osaba has served as a program committee member in more than forty international conferences and participated in organizing activities in more than ten international conferences. He is a member of the editorial board of the International Journal of Artificial Intelligence, Data in Brief, and Journal of Advanced Transportation. He is also a guest editor for the Journal of Computational Science, Neurocomputing, Swarm, and Evolutionary Computation and IEEE ITS Magazine.",institutionString:"TECNALIA Research & Innovation",institution:{name:"Tecnalia",country:{name:"Spain"}}},{id:"275829",title:"Dr.",name:"Esther",middleName:null,surname:"Villar-Rodriguez",slug:"esther-villar-rodriguez",fullName:"Esther Villar-Rodriguez",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/275829/images/system/275829.jpg",biography:"Dr. Esther Villar obtained a Ph.D. in Information and Communication Technologies from the University of Alcalá, Spain, in 2015. She obtained a degree in Computer Science from the University of Deusto, Spain, in 2010, and an MSc in Computer Languages and Systems from the National University of Distance Education, Spain, in 2012. Her areas of interest and knowledge include natural language processing (NLP), detection of impersonation in social networks, semantic web, and machine learning. Dr. Esther Villar made several contributions at conferences and publishing in various journals in those fields. Currently, she is working within the OPTIMA (Optimization Modeling & Analytics) business of TECNALIA’s ICT Division as a data scientist in projects related to the prediction and optimization of management and industrial processes (resource planning, energy efficiency, etc).",institutionString:"TECNALIA Research & Innovation",institution:{name:"Tecnalia",country:{name:"Spain"}}},{id:"49813",title:"Dr.",name:"Javier",middleName:null,surname:"Del Ser",slug:"javier-del-ser",fullName:"Javier Del Ser",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/49813/images/system/49813.png",biography:"Prof. Dr. Javier Del Ser received his first PhD in Telecommunication Engineering (Cum Laude) from the University of Navarra, Spain, in 2006, and a second PhD in Computational Intelligence (Summa Cum Laude) from the University of Alcala, Spain, in 2013. He is currently a principal researcher in data analytics and optimisation at TECNALIA (Spain), a visiting fellow at the Basque Center for Applied Mathematics (BCAM) and a part-time lecturer at the University of the Basque Country (UPV/EHU). His research interests gravitate on the use of descriptive, prescriptive and predictive algorithms for data mining and optimization in a diverse range of application fields such as Energy, Transport, Telecommunications, Health and Industry, among others. In these fields he has published more than 240 articles, co-supervised 8 Ph.D. theses, edited 6 books, coauthored 7 patents and participated/led more than 40 research projects. He is a Senior Member of the IEEE, and a recipient of the Biscay Talent prize for his academic career.",institutionString:"Tecnalia Research & Innovation",institution:null},{id:"278948",title:"Dr.",name:"Carlos Pedro",middleName:null,surname:"Gonçalves",slug:"carlos-pedro-goncalves",fullName:"Carlos Pedro Gonçalves",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRcmyQAC/Profile_Picture_1564224512145",biography:'Carlos Pedro Gonçalves (PhD) is an Associate Professor at Lusophone University of Humanities and Technologies and a researcher on Complexity Sciences, Quantum Technologies, Artificial Intelligence, Strategic Studies, Studies in Intelligence and Security, FinTech and Financial Risk Modeling. He is also a progammer with programming experience in:\n\nA) Quantum Computing using Qiskit Python module and IBM Quantum Experience Platform, with software developed on the simulation of Quantum Artificial Neural Networks and Quantum Cybersecurity;\n\nB) Artificial Intelligence and Machine learning programming in Python;\n\nC) Artificial Intelligence, Multiagent Systems Modeling and System Dynamics Modeling in Netlogo, with models developed in the areas of Chaos Theory, Econophysics, Artificial Intelligence, Classical and Quantum Complex Systems Science, with the Econophysics models having been cited worldwide and incorporated in PhD programs by different Universities.\n\nReceived an Arctic Code Vault Contributor status by GitHub, due to having developed open source software preserved in the \\"Arctic Code Vault\\" for future generations (https://archiveprogram.github.com/arctic-vault/), with the Strategy Analyzer A.I. module for decision making support (based on his PhD thesis, used in his Classes on Decision Making and in Strategic Intelligence Consulting Activities) and QNeural Python Quantum Neural Network simulator also preserved in the \\"Arctic Code Vault\\", for access to these software modules see: https://github.com/cpgoncalves. He is also a peer reviewer with outsanding review status from Elsevier journals, including Physica A, Neurocomputing and Engineering Applications of Artificial Intelligence. 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Currently working as an Assistant Professor in the Department of Mathematics, Institute of Applied Science, Mangalayatan University, Aligarh. She taught so many courses of Mathematics of UG and PG level. Her research Area of Expertise is Functional Analysis & Sequence Spaces. She has been working on Ideal Convergence of double sequence. She has published 17 research papers in National and International Journals including Cogent Mathematics, Filomat, Journal of Intelligent and Fuzzy Systems, Advances in Difference Equations, Journal of Mathematical Analysis, Journal of Mathematical & Computer Science etc. She has also reviewed few research papers for the and international journals. 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