Comparison of the nutritional quality of vermicompost and farmyard manure.
\\n\\n
More than half of the publishers listed alongside IntechOpen (18 out of 30) are Social Science and Humanities publishers. IntechOpen is an exception to this as a leader in not only Open Access content but Open Access content across all scientific disciplines, including Physical Sciences, Engineering and Technology, Health Sciences, Life Science, and Social Sciences and Humanities.
\\n\\nOur breakdown of titles published demonstrates this with 47% PET, 31% HS, 18% LS, and 4% SSH books published.
\\n\\n“Even though ItechOpen has shown the potential of sci-tech books using an OA approach,” other publishers “have shown little interest in OA books.”
\\n\\nAdditionally, each book published by IntechOpen contains original content and research findings.
\\n\\nWe are honored to be among such prestigious publishers and we hope to continue to spearhead that growth in our quest to promote Open Access as a true pioneer in OA book publishing.
\\n\\n\\n\\n
\\n"}]',published:!0,mainMedia:{caption:"IntechOpen Maintains",originalUrl:"/media/original/113"}},components:[{type:"htmlEditorComponent",content:'
Simba Information has released its Open Access Book Publishing 2020 - 2024 report and has again identified IntechOpen as the world’s largest Open Access book publisher by title count.
\n\nSimba Information is a leading provider for market intelligence and forecasts in the media and publishing industry. The report, published every year, provides an overview and financial outlook for the global professional e-book publishing market.
\n\nIntechOpen, De Gruyter, and Frontiers are the largest OA book publishers by title count, with IntechOpen coming in at first place with 5,101 OA books published, a good 1,782 titles ahead of the nearest competitor.
\n\nSince the first Open Access Book Publishing report published in 2016, IntechOpen has held the top stop each year.
\n\n\n\nMore than half of the publishers listed alongside IntechOpen (18 out of 30) are Social Science and Humanities publishers. IntechOpen is an exception to this as a leader in not only Open Access content but Open Access content across all scientific disciplines, including Physical Sciences, Engineering and Technology, Health Sciences, Life Science, and Social Sciences and Humanities.
\n\nOur breakdown of titles published demonstrates this with 47% PET, 31% HS, 18% LS, and 4% SSH books published.
\n\n“Even though ItechOpen has shown the potential of sci-tech books using an OA approach,” other publishers “have shown little interest in OA books.”
\n\nAdditionally, each book published by IntechOpen contains original content and research findings.
\n\nWe are honored to be among such prestigious publishers and we hope to continue to spearhead that growth in our quest to promote Open Access as a true pioneer in OA book publishing.
\n\n\n\n
\n'}],latestNews:[{slug:"intechopen-supports-asapbio-s-new-initiative-publish-your-reviews-20220729",title:"IntechOpen Supports ASAPbio’s New Initiative Publish Your Reviews"},{slug:"webinar-introduction-to-open-science-wednesday-18-may-1-pm-cest-20220518",title:"Webinar: Introduction to Open Science | Wednesday 18 May, 1 PM CEST"},{slug:"step-in-the-right-direction-intechopen-launches-a-portfolio-of-open-science-journals-20220414",title:"Step in the Right Direction: IntechOpen Launches a Portfolio of Open Science Journals"},{slug:"let-s-meet-at-london-book-fair-5-7-april-2022-olympia-london-20220321",title:"Let’s meet at London Book Fair, 5-7 April 2022, Olympia London"},{slug:"50-books-published-as-part-of-intechopen-and-knowledge-unlatched-ku-collaboration-20220316",title:"50 Books published as part of IntechOpen and Knowledge Unlatched (KU) Collaboration"},{slug:"intechopen-joins-the-united-nations-sustainable-development-goals-publishers-compact-20221702",title:"IntechOpen joins the United Nations Sustainable Development Goals Publishers Compact"},{slug:"intechopen-signs-exclusive-representation-agreement-with-lsr-libros-servicios-y-representaciones-s-a-de-c-v-20211123",title:"IntechOpen Signs Exclusive Representation Agreement with LSR Libros Servicios y Representaciones S.A. de C.V"},{slug:"intechopen-expands-partnership-with-research4life-20211110",title:"IntechOpen Expands Partnership with Research4Life"}]},book:{item:{type:"book",id:"854",leadTitle:null,fullTitle:"Micromachining Techniques for Fabrication of Micro and Nano Structures",title:"Micromachining Techniques for Fabrication of Micro and Nano Structures",subtitle:null,reviewType:"peer-reviewed",abstract:"Micromachining is used to fabricate three-dimensional microstructures and it is the foundation of a technology called Micro-Electro-Mechanical-Systems (MEMS). 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Plants have been used for many centuries with the purpose of feeding populations worldwide and to establish or bring back health, well-being, and the cure for several illnesses. The use of medicinal plants is very advantageous in terms of resource on chemical and biological research in natural products area. The plant secondary metabolism yields a wide range of chemical compounds, most of them highly bioactive and whose structural diversity is continuously evolving together with plants [1]. In vegetables, these compounds are the main responsible for chemical defence against fungi, phytopathogens, birds, and other natural predators, being also used by plants to attract pollinators as well, being indispensable to guarantee plant’s survival and its spreading through the globe. However, human population takes advantage of these remarkable properties and uses some compounds produced by diverse organisms including plants, fungi, and sponges to develop new medicines. Those metabolites coming from natural sources will promote the desirable healing action, bringing fewer side effects to the users.
In addition to teas, infusions, plasters, and herbal medicines, many traditional “western drugs” that are widely used nowadays had its origins on medicinal plants, such as (1) aspirin (acetylsalicylic acid—
Important drugs from medicinal plants: aspirin (1), artemisinin (2), and taxol (3).
Medicinal plant species constitute a valuable alternative to conventional medicine in many developing countries; especially in poor communities that inhabit rural areas, lacking access to health services. Several of them use plants as the primary health care, as teas, plasters, infusions, and ointments among others. The traditional use of medicinal plants and natural remedies with no established efficacy and safety is a widespread in many countries around the world. Accordingly, all the information about ethnobotany is of utmost importance: this kind of millenary knowledge built during centuries usually combines information from native indigenous culture, together with acquirements brought by the Europeans and the Africans and provides a more rational use for the local biodiversity.
In other hand, protozoan diseases represent an important health threat in countries of tropical and subtropical regions, causing mortality to their populations [3]. Many neglected tropical diseases (NTDs) transmitted by parasites are reported to have life cycles including man as a secondary host, in which they cause disease. About 37 million individuals are presently infected by parasites around the world. Together with malaria and amoebiasis, the parasitic illnesses are the main cause of thereabout one million deaths per year. Infections caused by protozoan species such as
Leishmaniasis, Chagas’ disease, and human African trypanosomiasis (HAT) are among the most important protozoan parasitic illnesses caused by trypanosomatids. Chagas’ disease, also known as American trypanosomiasis, is a widespread disease, caused by the kinetoplastid protozoan Tripanosome—
That’s why Chagas is recognized as one of the most devastating diseases caused by the parasites of the
This chapter is therefore aimed to review the great potential of natural products that are available in nature (mainly plants and sponges) regarding to the prevention and treatment of Chagas’ disease and the combat of triatomine bugs.
Neglected tropical diseases (NTDs) are often chronic and debilitating illnesses that currently affect over one billion people worldwide. NTDs are a diverse group of infectious diseases that affect primarily rural and low-income populations residing in tropical and subtropical regions worldwide. The World Health Organization (WHO) officially recognized nowadays 17 NTDs, comprising a highly diverse group of bacterial, protozoan, and helminth infections, transmitted via insects, contaminated food, water, and soil, and/or through human-to-human contact. These diseases cause easily over 200,000 deaths per year affecting many millions more around the globe, although the number of new infections appears to be dwindling. NTDs include the three major protozoan diseases: human African trypanosomiasis (HAT or “sleeping sickness”), Chagas’ disease, and leishmaniasis [5]. Dengue, foodborne trematodiases, leprosy, lymphatic filariasis, schistosomiasis, soil transmitted helminthiasis, and trachoma [6] are also classified as NTDs.
The socioeconomic impact of NTDs in the developing countries surpasses that of any other infectious disease (with exception of HIV/AIDS) and perhaps may have permanent socioeconomic effects on many nations. It is such a waste that billions of dollars of productivity are lost to NTDs every year in treatment and prevention costs, besides bearing 149 countries plus the information that the threat of NTDs is no longer confined to nations where these diseases are endemic. Due to globalization and the increasing social, financial, and technological connectedness, the burden to carry NTDs has become global issues.
Massive efforts of community activists, health care workers, scientists, politicians, and economists are required to reduce significantly the significance of public health liability that NTDs oblige. The most effective approach for reducing these diseases is still prevention, due to the absence of affordable or effective curative therapies and the deficiency of preventive vaccines. Between such relevant public health issues and many lives directly or indirectly affected by NTDs, there is education that offers a solution to connect NTD prevention to treatment efforts [7].
Trypanosomiasis is a group of parasitic diseases caused by protozoan from
In 1909, the Brazilian physician and researcher Carlos Chagas discovered the etiologic agent of American trypanosomiasis
Fever, headache, enlarged lymph glands, and swelling of the eyelid, close to the site of the bite of the insect, are some of the more common mild symptoms of the initial American trypanosomiasis acute phase. This infection is characterized by two distinct clinical stages: the acute phase, with high parasitemia, commonly progresses to a subsequent state of latency, and the chronic phase, with clinical manifestations in various organs. The most common symptoms characteristic of the chronic phase are enlargement of heart ventricles and enlarged esophagus or colon [13], and these manifestations are occasionally life threatening [14].
Chagas’ infection has a wild cycle in nature that exists for millions of years. It is believed that some accidental cases involving humans might have happened at the time, similarly as they occur nowadays: when mankind invades vectors’ wild ecotope or when triatomine bugs invade human domiciles. However,
Triatomines have been known since the sixteenth century but they have only settled down on human households with the beginning of the agricultural cycle. The increasing deforestation through the centuries that marked the livestock cycle leads to the removal of the native animals that once were the main sources of nourishment for the triatomines. Hence, these bugs have adapted progressively to inhabit areas surrounding human residences and the interiors of these dwellings. When humans invaded wild ecotopes and became infected, the transmission of Chagas’ disease ceased to be treated as an enzootic disease of wild animals and is so called anthropozoonosis [15].
It is reported for
In this way, it is possible to perceive that
Life cycle of
After triatomines bite an infected mammalian, they ingest the trypomastigotes form of
It is reported that the transmission mechanisms for Chagas’ infection can be divided into two distinct groups [9]:
Principal mechanisms: by means of triatomines (representing around 70% of the cases), blood transfusion (up to 20% of the cases), oral transmission, contaminated food, and placental or birth canal transmission;
Secondary mechanisms: by means of management of infected animals, organ transplants, laboratory accidents, wounds, sexual transmission, contact with menstrual fluid, or sperm contaminated with parasites, and also, the hypothetic cases of purposeful criminal inoculation and contamination of food with
The challenge on searching for new Chagas’ disease drugs remains for decades. Nowadays, the usual recommended traditional treatment is chemotherapic including either one of the two nitro-aromatic heterocyclic compounds (Figure 3) benznidazole (4) and nifurtimox (5).
Recommended chemotherapic drugs (4) and (5) used on treatment for Chagas’ disease.
As cited previously, this infection is clinically characterized by two distinct stages: the acute usually asymptomatic phase, defined by high parasitemia, and a long chronic and progressive phase in which symptoms can manifest after some years. When the patient is in the acute phase of the infection, the treatment with these drugs can cure up to 80% of the cases. Depending on medical orientation, drugs benznidazole (4) and nifurtimox (5) can be administered either separately or simultaneously. However, on the treatment of patients in the chronic phase, drug efficacy decreases dramatically curing only 5–20% of the cases. In addition to this limited therapeutic potential, both compounds feature high toxicity [3].
There are many papers discussing the limitations of the conventional therapeutic approach [10, 12]:
the high dosages of drugs used and the long duration of the treatment, both necessary to produce the desired medicinal effect;
the ineffectiveness of such drugs against all the stages of the disease and all strains of the parasite;
problems related to the lack of efficiency in drugs’ production and distribution;
several toxic effects carried out by these drugs on the patients;
their limited effectiveness during the chronic stage;
regional degrees of effectiveness due to drug resistance and;
the presence of severe side effects leading to the immediate interruption of treatment in a high percentage of the patients.
All those reasons highlight the urgent need for research on new Chagas’ drugs and/or safer alternative treatments.
Through the last decades, many efforts have been made, aiming for an effective treatment for Chagas’ disease without major prejudice to patients’ health. There were meritorious advances regarding to molecular biology field and pathophysiology of Chagas’ disease. However, according to Coura and Viñas [14], those efforts were yet unsuccessful due to:
the usual lack of symptoms in the illness’ acute phase;
the occurrence of various parasites strains (with different drug resistance profile);
the hardness to find a selective and more suitable drug for the parasites and;
the inefficient fund distribution for research while most of investments are aimed to prevention and to develop diagnostic tests.
Most of the current knowledge about parasites’ biology, the identification of potential molecular targets, together with the potential natural molecules from the plant kingdom, has encouraged researchers to keep searching sorely for new drugs against
Nature is a skilled factory that produces a wide variety of chemical substances with broad structural patterns that researchers call as natural products. Most of them are secondary metabolites synthesized by plants that are directly or indirectly related to their vital processes from metabolism to chemical defense and every single way that vegetables relate to the environment.
Searching in the literature, it is possible to find many works about broad classes of secondary metabolites that have proven to be active against
Bioactive natural compounds despite being very attractive sources for new drugs in their original form can also be subjected to derivatization reactions or via synthetic steps, aiming to change chemically functional groups to magnify their bioactivity [14]. In this way, many classes of secondary metabolites, pure compounds, and its derivatives have been specifically tested
Abdel-Sattar and co-workers [8] investigated the
A few other methanolic extracts showed moderate activity while 20 were considered to be inactive against
Another investigated Solanaceae is
Physalins A (6), B (7), D (8), F (9), and G (10) isolated from
Some results are very promising though one of the major problems faced by many research groups on natural products chemistry worldwide is related to the difficulty to obtain pure active secondary metabolites from natural sources. This fact could not be different for physalins: to isolate these compounds and obtain them in the pure form, it is quite difficult and time consuming, usually affording low yields at high costs. So economically, it can become very unattractive to treat any NTDs using pure isolated plant compounds like physalins for example.
On the other hand, the use of potential compounds from natural sources usually presents good alternative. Activity assays were performed on crude ethanolic extract of
Furthermore, the presence of phenolic compounds (Figure 5) like chlorogenic acid (11), rosmarinic acid (12), and coumarin (13) and flavonoids (Figure 6) luteolin (14), kaempferol (15), and vitexin (16) in low concentrations may have been responsible for the weak bioactivity of
Active phenolic compounds (11), (12) and (13) from ethanol extracts of
Active flavonoids (14), (15), (16) and (17) from ethanol extracts of
In
Several
The steroidal glycoalkaloid α-solamargine (18) was found on the ripe fruits of
An early phytochemical analysis showed a very similar profile of secondary metabolites for both species extracts, revealing the presence of triterpenes, phenols, saponins, flavonoids, coumarins, and anthocyanosids on polar extracts. The authors found that biological activity of
Based on activity observed for dichloromethane and ethanol extracts of
More than 20,000 known compounds are triterpenoids produced by plants through squalene cyclization. The terpenes are considered to be the most representative group of phytochemicals [21] being the structural base for several classes of derivatives. Hence, compounds from these classes are very abundant in nature being an attractive group to be screened for biological activities of interest. Hundreds of new terpene-derived molecules exhibiting trypanocidal activity have been described on the past 10 years; some of them have already been assayed
The diterpenoids with an abietane-type skeleton (Figure 8) present in many plants are known to possess a wide range of biological activities, including anti-inflammatory, antibacterial, antifungal, and antimalarial among others. For example, the phenolic abietane ferruginol (20), isolated from the roots of the herb
Active terpenoids (20, 21, and 22) and triterpenes (23 and 24) isolated from plants.
The triterpenes ursolic acid (23) and oleanolic acid (24) obtained in their pure form from
The sesquiterpene caryophyllene (25) and the phenylpropanoid eugenol (26) can be found in nature on many essential oils (Figure 9). Both were tested
Structures of active compounds: caryophyllene (25) and eugenol (26).
Caryophyllene (25) showed higher percentage of parasite inhibition, being capable of eliminate 100% of
Sesquiterpene lactones (Figure 10) are terpenoid derivatives and usually have α,β-unsaturated carbonyl groups that are primarily responsible for mediate their wide spectrum of biological activities. Many compounds from this chemical class often show high activity against
Sesquiterpene lactones: dehydroleucodine (27) and thapsigargin (28).
Interestingly, some of these terpenic molecules are feasible to chemical modification in order to comprehend their mechanisms of action in such organisms or intended to optimize their effectiveness on elimination of parasites. It is possible to strategically perform chemical reactions on specific functional groups on some known natural products. This approach proved to be very effective, once with the increasing on lipophilicity of isolated diterpenes lead to a substantial improvement on their trypanocidal activity, for example. It is also reported that parasites have a rudimentary defence system highly sensitive to oxidative stress, being their main vulnerability [14].
In addition to terpenoids, other group of natural products with very interesting bioactivity is the flavonoids (Figures 6 and 11). They are very abundant in nature being responsible for many interesting properties like antioxidant, anti-inflammatory, and free-radical scavengers. The ethanol leaf extract from the bay cedar,
Flavanones: naringenin (29), sakuranetin (30), and sakuranetin-4′-methyl ether (31).
Flavanones (Figure 11) naringenin (29), sakuranetin (30), and its methylated derivative sakuranetin-4′-methyl ether (31) have their antiparasital activity tested
In this study, the authors reported that sakuranetin (30) presented good activity against all tested
Lectin is the name given to a group containing all sugar-specific agglutinins of nonimmune origin. Those substances were found to be valuable because they could recognize and bind carbohydrates specifically and reversibly. Hence, the lectins have great potential and value in the study of glycoproteins, helping to comprehend the mechanisms of many physiological and pathological processes [25]. The bonding between lectins and some protozoans’ sugars is believed to cause interference in chemical or biological processes that eventually lead to the death of these parasites. Therefore, lectin isolated from triatomine insect
It is evident that many medicinal plants from
Among the major constituents identified on
Some chemical constituents (32 – 51) of active plants essential oils.
Usually the collection of wild herbal populations can result in extracts and essential oils with variable compositions [10]. So, after
Essential oils extracted from fresh leaves of velame,
The crescent need for bioactive molecules that can be used as potential natural drugs, being able to cure diseases and reducing undesirable side effects at the same time, leads the researches all around the world to look to the sea. Many papers available in the literature report the search for new active compounds, and they have found that marine biodiversity is a promising source of natural products with remarkable biological activities. To the best of our knowledge, studies involving marine sponges yield close to 200 new pharmacologically active metabolites every year [28].
Being ancient organisms, some sponges contain diverse groups of metabolically active compounds. Hence, the investigation of biological activity is an important source to obtain extracts or compounds with potential biomedical action. So much that the effect of acetone extract from lyophilized Brazilian and Spanish marine sponges,
Steroids stigmasterol (52),
The trypomastigotes were sensitive to the presence of different concentrations of marine sponge extracts as well. Although the action mechanism of steroids is unknown, it is accepted that these compounds may be initiated at the cell membrane but also via intracellular receptor binding. In addition, steroids may participate in growth regulation, proliferation and cell death, and redox mechanisms [12]. These compounds could participate in a conjugated addition of nucleophilic amino acid residues present in target enzymes on
As discussed through this chapter, triatomine bugs can affect human health acting as vectors transmitting Chagas’ disease to many populations worldwide. The inappropriate use of synthetic insecticides, usually been used to control these insects, is closely linked to the development of resistance in pests, human diseases, and contamination of food and the environment. Resistance to the pyrethroid deltamethrin and other nonnatural insecticides, for example, has been reported in different areas of the Gran Chaco region of Argentina and Bolivia for
For example, Nieto-Sanchez
the active search and elimination of triatomines;
insecticide-based fumigation on infected places;
educational activities managing population.
Those prevention methods are effective in short term for reducing triatomine infestation, although do not prevent reinfestation in the long run. Interestingly, they have also reported practices such as sweeping with brooms made from plants believed to have natural insecticide properties by local residents: herbs such as porotillo (
Those findings suggest that multiple tasks are required to control bug recolonization, especially in poorly constructed houses. The usual use of synthetic chemical insecticides constitutes a fragile short-term solution for controlling Chagas’ disease. There is a need to develop more sustainable long-lasting solutions for Chagas’ disease transmission in areas that have high occurrence of triatomine infestation.
The studies reviewed briefly in this chapter along with many others that have been carried out since the 50s have brought valuable information aiming to contribute to the understanding about the parasite’s life cycle and to highlight the crescent need to clarifying some biomolecular targets and enzymatic mechanisms that could be useful to the development of new natural drugs against
It is personally believed that the cure for Chagas’ disease is hidden somewhere in nature; the scientists are currently working as explorers, prospecting this greatness in molecular levels, searching in every single bush for a viable solution that helps populations suffering for Chagas worldwide. Here in few lines, it was showed the great potential of natural products for the treatment of this parasitic disease. The plentiful Mother Nature furnishes material to the obtention of useful substances emerging from crude extracts, essential oils, and many fractions possessing very complex, variable, and rich composition. In this chapter, was portrayed, several groups of secondary metabolites, such as diterpenes, terpenes, triterpenes, sesquiterpenes, sesquiterpene lactones, steroids, flavonoids, polyketides, lectins, and many others.
Massive efforts of community activists, health care workers, politicians, and economists are also required to reduce significantly the significance of public health liability that NTDs oblige. The most effective approach for reducing these diseases is still prevention, due to the absence of affordable or effective curative therapies and the deficiency of preventive vaccines. Between such relevant public health issues and many lives directly or indirectly affected by NTDs, there is education that offers a solution to connect NTD prevention to treatment efforts.
NPV would like to thank her sister Karina Pacheco Vaz for drawing the scheme in Figure 2 and for the final art for all the figures available on this chapter.
Most of the aquaculture productions in developing countries are practiced in rural areas using semi-intensive fish pond culture systems. This rural aquaculture plays a major role in improving livelihoods, enhancing social equity, advancing gender equality, contributing to global food production (food and nutritional security), and promoting regional economies. Consequently, aquaculture helps curb the high rate of malnutrition occasioned by undernourishment in most rural setups (particularly in Africa) since the superior nutrition in fish improves health by providing food and supporting both mental and physical development and functioning [1]. Besides, unlike some animal protein sources, fish and fish by-products are widely accepted among various social, cultural, and religious backgrounds [2]. Moreover, in developing countries where people have the highest share of fish protein in their diets, the aquaculture sector plays a vital role in maintaining reasonable livelihoods through food and nutrition provision as well as job creation.
Nevertheless, the significant role played by aquaculture and the increasing demand of aquatic organisms as well as the advancement in science and technology, fish farming in most developing countries is still at infancy stages, being practiced secondary and part-time to agriculture.
Fish feed challenge has been identified as one of the limitations to the commercialization of aquaculture, particularly in Africa. This is because farmers prefer and highly depend on fishmeal as the main protein source due to its superior nutritional properties, palatability, and biological value. Consequently, the majority of the farmers do not achieve their full potential due to the cost, scarcity, and ecological implications associated with obtaining the fishmeal.
The majority of rural fish farming is undertaken in semi-intensive culture systems using earthen (polythene lined or cemented) ponds with limited or no supplementary feeding. Therefore, pond fertilization is taken as a crucial step in pond management to promote phytoplankton production that is the key link to the fish food chain. Studies have considered this old age undertaking as a cost-cutting practice that has the potential of enhancing the fish yields up to 2.8 times [3]. Fertilization is undertaken to improve the growth and reproduction of natural food organisms, which include zooplanktons, phytoplanktons, bacteria, microscopic algae, and insects. These natural organisms in water are eaten directly or indirectly depending on the fish-eating behaviors. For example, the herbivorous fish (such as carps) feed on algae and bacteria biomass, the carnivores (such as catfish) consume zooplanktons and insects, while omnivorous fish (such as tilapia) feed on all.
Therefore, when a pond is fertilized for example using manure, some portion is assimilated or stored by the phytoplanktons, while another share is consumed directly by zooplanktons and fish. Another portion goes to pond bottom where it combines with organic matter to promote the development of the microbial community, which apart from being fed by fish, the bacteria and fungi play a crucial role in ponds of decomposing uneaten fish feeds and toxic wastes [3]. There is also another portion of the manure that is adsorbed by pond sediment whereby it improves bottom soil quality and its water retention properties, and in case of decreased nutrients in the pond, these manures are released slowly back to the water body to promote the plankton development.
The fertilizers used are either organic or inorganic. The large-scale fish ponds are disposed to using inorganic fertilizers thanks to their easy storage and distribution as well as consistent and high nutritional contents. However, their utilization is limited by their scarcity, high cost of obtaining them, and the risks of impairing soil fertility, causing environmental degradation through the pollution to receiving waters, CO2 generation, and depletion of fossil fuels. Furthermore, chemical fertilizers if not used properly can cause fish toxicity by decreasing dissolved oxygen concentration and increasing total dissolved solutes, alkalinity, conductivity, and free carbon dioxide [4]. Besides, chemical fertilizer application beyond certain levels leads to decreased pond’s natural productivity due to self-shielding of sunlight by the upper layer of dense crops of phytoplanktons.
On the other hand, small-scale farmers depend on organic fertilizers from agricultural wastes of crops and livestock animals such as cow dung and chicken droppings. Unlike chemical fertilizers, organic manures are cheap and locally available, contain organic matter, can be directly consumed by fish, and improve soil structure and water retention. In addition, the straw-like particles in organic manure provide an attachment substrate for microbes to flourish. In Israel, Schroeder [5, 6] demonstrated that organic manure application in fish culture yields three times more than inorganic fertilizer. Nonetheless, organic manure nutritional content is variable and low and recently, they are becoming scarce and relatively expensive to obtain due to competition from crop and fuel production. In addition, cow manure increases biological oxygen demand (BOD) because of the high content of organic matter that consumes a lot of oxygen during their aerobic decomposition often leading to anorexic zones in pond bottoms. Further, livestock manure poses the risks of pathogen transfer to fish.
Therefore, there is a need to adopt economically and ecologically sustainable organic fertilizers such as vermicompost, which has no biosafety concerns. The vermicomposting biotechnology takes advantage of the voracious, polyphagous, fast-growing, and high reproduction nature of earthworms to consume large amounts of organic waste and excrete vermiwastes. The vermicomposting products are earthworm biomass and vermiwastes: vermicompost (solid waste) and vermiliquid (liquid waste). The earthworm biomass is either fed directly to aquatic organisms or used as the protein source in formulating fish diets [7, 8, 9]. Nonetheless, the earthworms biomass utilization in fish feed production is limited by processing challenges (handling, harvesting, and gut content evacuation) and the presence of anti-nutritional factors (coelom fluid and chitin), which inhibit uptake, digestion, and assimilation by aquatic organisms [8, 9]. The vermiwastes have also been used in formulating fish feeds; however, their utilization is equally limited by relatively low protein contents and processing impediments [10, 11, 12]. In addition, vermicompost utilization is still limited by its efficacy and undeveloped market when compared to compost and chemical fertilizers.
Therefore, this study reviews various applications of the vermiwastes in promoting aquaculture nutrition by improving pond primary production. This is to provide farmers with simple, easily available, and cost-effective fertilizer that can increase fish production and resource utilization without compromising water quality, health standards as well as environmental integrity.
Earthworms are ground-dwelling organisms, which belong to the phylum
In this biotechnology, the earthworms are made to consume large quantities of organic materials, which undergo a biochemical process in the worm’s gut before being deposited as an excreta known as the vermicompost or vermicast or vermiwastes. This complex biological and ecological process of vermicomposting is enhanced by the presence of microorganisms, mucus, and enzymes in the gut of the earthworms to produce a stable and safe compost that contains and can hold more nutrients for over an extended period of time without affecting the environment. The excreta is rich in humus, which contains micro and macronutrients, antibiotics, vitamins, microbes, growth promoters, and fungal communities [13].
The commonly used omnivorous earthworm species in vermicomposting are
The culture substrates for earthworms comprises a wide range of organic materials that can promote the production of fungi, protozoa, and bacteria that are the highly preferred diets of the worms. Earthworms feed on different food materials, such as livestock manure wood, leave, crop and kitchen wastes, sewage sludge, and agro-industrial waste. The commonly used substrates are livestock manure and kitchen wastes. However, the kitchen wastes of onions and meat should be avoided because they kill fish by reducing pH and suffocation, respectively.
Depending on the culture substrate and worms species used, the vermicompost is usually ready within 2 to 4 months, but the vermiliquid can be harvested on daily basis. There are several techniques of separating the earthworms from the compost but the most convenient one is to, stop sprinkling the substrate with water, introduce light from the top side to prompt the worms to bury downwards, and then collect the upper vermicompost. Otherwise, one can starve the earthworm for few days then introduce a new feed substrate at the top prompting the worms to migrate upward then harvest the vermicompost at the base.
The harvested vermicompost has elevated nutrient levels, which are in ready-to-uptake form by plants (unlike compost that requires curing before use) because they are released relatively easily and faster, thus improving plankton and fish growth performance [14, 15, 16, 17, 18]. This is because the earthworms ingest large volumes of nitrogen-laden organic matter, mineralize it in their guts, and excretes it whereby it is stored in the vermicompost inform of nitrate that is more bioavailable to plants when compared to the ammonia form found in conventional compost [19, 20]. This mineralization of nitrates due to vermicomposting and the respiration by earthworms drops the C:N of fresh organic matter to the desired ratio of below 20:1 [12].
Apart from the vermicompost, the vermicomposting process produces vermiliquid, the leachate from the organic waste, and the vermicompost. The vermiliquid (also known as vermiwash or worm tea) just as the vermicompost is an equally nutritious fertilizer because it contains fecal excretion, organic materials, microorganisms, enzymes, earthworms secretions, mucus, and organic acids, and in addition, it contains soluble plant nutrients [21]. Vermiliquid has shown more potential to improve aquaculture nutrition because it further contains cocoons, body parts of worms, small and dead earthworms all that is edible to fish, and some zooplanktons and contributes to more nitrogen to the vermicompost [19, 22]. Besides, the vermiwash is known to contain bacterial biomass, hormones, antibiotics, free amino acids, vitamins (pro-vitamin B and D complex), metabolites suitable for fish growth, feed digestion, disease resistance, and immune boosters [16]. In addition, the vermiliquid has recommendable proteins contents with the potential of substituting basal ingredients in fish feed formulation [10, 11]. Musyoka et al. [12] demonstrated how a mixture of the three vermicomposting products (i.e. earthworms, vermicompost, and vermiliquid) also known as earthworm bedding has superior nutrition that is capable of economically replacing fishmeal in diets of Nile tilapia (
When compared to the traditional compost and other farmyard manures such as cow dung, horse dung, and poultry droppings, vermicompost has been found to be more nutritious, containing relatively good amounts of carbon, nitrogen, phosphorus, calcium, and C:N ratio as shown in Table 1. Nonetheless, the quality of these vermiwastes can be enhanced by using nutritious substrates (preferably a mixture of different susbtrates) and different species of earthworms. For example Musyoka et al. [12] used mixed substrates of kitchen waste, coffee husks, barley waste, and livestock manure and produced vermicompost with superior nutrition when compared to farmyard manure as shown in Table 1. Similarly, when Marsh et al. [24] added shredded cardboard to aquaculture effluent they produced a vermicompost with superior nutrition when compared to Rahman et al. [25] who used cow dung substrate alone as shown in Table 1.
Nutrients (%) | Vermicompost [23] | Vermicompost [12] | Vermicompost [24] | Vermicompost [25] | Farm yard manure [23] |
---|---|---|---|---|---|
Substrate used | — | Kitchen waste, coffee husks, barley waste, and livestock manure | Shredded cardboard and aquaculture effluents | Cow dung | |
Nitrogen | 1.6 | 1.06 | 2.7 | 2.65a | 0.5 |
Phosphorus | 0.7 | 0.35 | 1.6 | 2.21a | 0.2 |
Potassium | 0.8 | 0.66 | 2.7 | — | 0.5 |
Calcium | 0.5 | 4.13 | 8 | 1.83a | 0.9 |
Carbon | — | 12.9 | 40 | 8.76 | — |
C:N ratio | 15.5 | 13.57 | 15.8 | — | 3.3 |
Comparison of the nutritional quality of vermicompost and farmyard manure.
mg 100 g−1 manure.
Studies have shown vermicompost to contain up to seven times more nutritional and plant growth-promoting value thanks to the faster activation of humus when compared to conventional composting [26, 27]. Besides providing nutrition, the humic acid produced during vermicomposting has the ability to suppress the growth of harmful bacteria and fungi (particularly those responsible for mycotoxins production); thus when consumed by animals, it promotes gut health (for increased nutrient utilization), stress management, and immune systems and controls intestinal diseases [28]. Unlike the compost whereby nutrients such as nitrogen are denatured and microbes die due to high temperatures, vermicompost contain a rich microbial community (that remains unchanged even after drying the vermicompost), which has shown to be direct food to zooplanktons and fish as well as being beneficial in reducing pathogenic bacteria [29]. Kaur and Ansal et al. [30] recommended the development of biotechniques for isolating and harvesting of the beneficial microbial biomass present in vermicompost to be used as biocontrol. In addition, the presence of coelom fluid produced by earthworms (whenever they get agitated) makes vermicompost pathogen-free and goes a long way in protecting fish from diseases [29]. Moreover, the vermicomposting presents stable and mature organic matter rich in humic acids that reduce the harmful effects associated with toxic gases produced by undigested or semi-digested organic manure to fish. In addition, the earthworms reduce heavy metal concentrations on organic matter by baring them in their gut and skin whereby they are slowly broken down into non-toxic forms [31]. Further, the non-thermophilic vermicomposting produces fewer greenhouse gases when compared to traditional composting [32]. Other advantages of vermicomposting over traditional composting are, being a friendly technology that is simple, cheap, relatively faster, can be done indoors using locally available organic materials and using less technical expertise. Correspondingly, studies have reported vermicompost to have great potential of replacing inorganic fertilizer. Sinha et al. [33] reported the vermicompost potential to enhance crop growth by up to 30–40% when compared to chemical fertilizers.
In addition, vermicomposting promotes waste treatment and resource utilization particularly in rural areas whereby cheap underutilized agro-industrial residues are in abundance. Importantly, these residues are not directly consumed by humans (unlike fishmeal), hence cheap and have less competition; however, their utilization in aquaculture nutrition is inhibited by unbalanced nutrition, unpalatability, high fiber, the presence of anti-nutritional factors, and have bio-safety, processing, and ethical issues. Various studies have demonstrated the potential of vermicomposting to vaporize these residues to nutritious earthworm biomass, vermicompost, vermiliquid, and earthworm bedding (mixture of the three) [7, 8, 34]. Besides, vermiremediation has been shown to reduce organic solid wastes by up to 75% faster, BOD and TDSS by over 95% and significantly removing chemicals, heavy metal contaminants, and undesirable gases such as H2S, NH3 [35, 36]. With the harvesting and processing challenges of earthworms as well as their bioaccumulation of toxic organic and heavy metals, the utilization of vermicompost in aquaculture has been seen as a suitable alternative [37]. Moreover, the utilization of vermitechnology has also been promoted by the preference to consume organically produced foods, the need to conserve energy, control organic waste pollution, and optimize economical resource utilization strategies.
The phytoplanktons, zooplanktons, benthos, microbes, and detritus are natural foods for aquatic organisms. These planktons are the key link to the food chain of aquatic ecosystems as they are the naturally preferred feeds by fish particularly the juveniles. Any artificial food in fish ponds is only required to supplement the deficiency of natural feeds in terms of quantity and quality. Just like most conventional artificial fish feed sources, the planktons have protein contents ranging between 40 and 60%, which is optimal for culturing fish [38]. Therefore, promoting the productions of planktons to adequate amounts can contribute to the overall development of fish in ponds and would mean less or none of the artificial feeds.
With the intensification of aquaculture, the abundance and diversity of planktons are augmented by fertilizer application. Intense fertilization has been shown to yield between 15 and 32 kg/ha/day with no additional supplemental feed in semi-intensive fish farming, translating to 100% replacement of conventional feed [6]. The vermicompost manure has been credited as potential pond fertilizer because it is nutritious enough to supply nutrients to the planktons [17, 30]. In vermicompost manured ponds, Kumar et al. [39] noticed a correlation between improved phytoplankton biomass and zooplankton abundance and diversity, with 68.38, 19.77, and 11.38% occurrence of rotifers, cladocerans, and copepods, respectively. These authors recommended a vermicompost application rate of 5000 kg/ha/year for optimum water qualities. Consequently, the vermicompost has been recommended as suitable manure for nursery pond management to provide zooplanktons that are the preferred feed diets by fingerlings. Habibnia and Bahram [40] observed improved growth and survival of Rutilis kutum (
On average, the majority of the studies have recommended a vermicompost manure application rate of 10,000 kg/ha/year for optimal water quality parameters, and maximum plankton and fish performance as shown in Table 2.
Vermicomposting substrate used | Parameters tested | Fish tested | Recommended application rate | Author (s) |
---|---|---|---|---|
Cow dung | Water quality, fish growth performance, and plankton biomass | 10,000 kg/ha/year | [42] | |
Cow dung and poultry manure | Water quality, zooplankton production, and growth performance | 15,000 kg/ha/year | [30] | |
Cow dung | Growth performance | Labeo rohita | 10,000 kg/ha/yr | [41] |
Water hyacinth ( | Water quality, plankton production, and fish growth performance | 3970.56 kg/ha/90 days | [15] | |
Water hyacinth ( | Plankton abundance and diversity and fish growth performance | 3970.56 kg/ha/90 day | [16] | |
Cow dung | Growth performance of fish, water quality, and plankton production | 4000.00 kg/ha/90 days | [17] | |
Solid municipal waste | Water quality, soil retention, and fish growth performance | Cat fish, | 15,000 kg/ha/year | [43] |
Livestock manure | Water quality parameters and plankton biomass | 10,000 kg/ha/year | [29] | |
Cow dung | Water quality, plankton production, and fish growth performance | 5550 kg/ha/105 days | [25] | |
Cow dung, pig manure, and poultry manure | Growth performance of fish | 10,000 kg/ha/yr | [44] | |
Cow dung | Growth performance | 10,000 kg/ha/year | [18] | |
Cow manure | Plankton abundance, growth indices, and survival | 10,000 kg/ha/year | [40] | |
Cow dung | Fish growth performance and plankton biomass | 10,000 kg/ha/yr. | [45] |
Utilization of vermicompost in fish ponds.
Further, the vermicompost provides food directly to zooplanktons and fish [39]. Though the vermicompost alone might contain low protein value for feeding fish directly, the microbes adhering to organic manures improve their nutrition to contents suitable for the majority of aquatic organisms. Rahman et al. [25] fertilized monosex
Importantly, vermicompost has been shown to improve aquatic organisms’ survival and growth performance without compromising water quality [30, 41, 46]. Kaur and Gupta [45] indicated that vermicompost fertilizer did not alter the physicochemical properties of pond water and improved the growth performance of both planktons and fish. Likewise, Ansal et al. [47] and Kaur and Ansal [30] reported significantly increased dissolved oxygen concertation in fish ponds fertilized with vermicompost when compared to those fertilized with other organic fertilizers.
With the improved plankton biomass, water quality, the vermicompost manure subsequently produces significantly high fish growth performance. After fertilizing ponds with vermicompost, Godara et al. [29] observed significant high growth of catla (
Just like most organic manures, vermicompost is recommended over inorganic fertilizers. Chakrabarty et al. [15] observed significantly high production of plankton diversity and
On the other hand, the vermiliquid has equally shown the prospects of directly improving plankton productivity, particularly zooplanktons such as
It is interesting to note that a symbiotic system is developed when vermiculture is integrated with aquaculture; the pond wastes provide feed substrates to earthworms, and in return, the earthworms consume and excrete them as biofertilizers for improving fish pond natural productivity. Studies have recommended vermicomposting biotechnology as a suitable bioremediation technic to treat and vaporize aquaculture wastes to stable and safe organic fertilizers and earthworm biomass, that can be reused for increasing pond primary productions and fish feed production, respectively [24, 48]. Aquaculture waste (principally from intensive recirculating systems) is known to be rich in organic matter due to the elevated nutrients in uneaten feeds and the waste products of the fish. The untreated wastes cause pollution, siltation and its use as fertilizer are problematic because they are susceptible to putrefaction and contains disease-causing agents [48]. Therefore, integrating vermiculture into fish farming is fundamental to not only provide nutrition but also help in the recycling of organic wastes including that of aquaculture itself.
Vermicomposting has been recognized as a natural and cheap biotechnique of treating and bio-transforming organic wastes to safe and steady biofertilizers with the potential to promote aquaculture nutrition. This is because vermicompost contains elevated nutrients, organic matter, microbial biomass, humic acid, and exchangeable cations suitable for supplying nutrients for phytoplanktons, providing food directly to zooplanktons and fish as well as improving immunity, disease resistance, and physiochemical properties of water and pond sediment quality. Besides, the biotechnology is highly considered over the traditional composting because it regulates, improves, enhances, and promotes itself, and it conserves nutrients and microbial communities, involves low or no energy, forms little or no sludge, releases minimal greenhouse gases, and is performed on-site using simple structures requiring minimal expertise. This is particularly significant in developing nations, whereby fish farmers are struggling to break-even (even with many underutilized resources) and have less technical know-how, and their purchasing power of chemical fertilizer and commercial fish feeds is very low. Therefore, integrating vermicomposting into fish-cum agricultural activities can not only improve fish yields but also improve water and resource management, thus promoting sustainable aquaculture, and improving food security and ecological balance.
This is a brief overview of the main steps involved in publishing with IntechOpen Compacts, Monographs and Edited Books. Once you submit your proposal you will be appointed a Author Service Manager who will be your single point of contact and lead you through all the described steps below.
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\n\nAfter approval, you will proceed in submitting your full-length manuscript. 50-130 pages for compacts, 130-500 for Monographs & Edited Books.Your full-length manuscript must follow IntechOpen's Author Guidelines and comply with our publishing rules. Once the manuscript is submitted, but before it is forwarded for peer review, it will be screened for plagiarism.
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\n\nIf the manuscript is formally accepted after peer review you will receive a formal Notice of Acceptance, and a price quote.
\n\nThe Open Access Publishing Fee of your IntechOpen Compacts, Monograph or Edited Book depends on the volume of the publication and includes: project management, editorial and peer review services, technical editing, language copyediting, cover design and book layout, book promotion and ISBN assignment.
\n\nWe will send you your price quote and after it has been accepted (by both the author and the publisher), both parties will sign a Statement of Work binding them to adhere to the agreed upon terms.
\n\nAt this step you will also be asked to accept the Copyright Agreement.
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Aalborg University has Two Satellite Campuses, one in Copenhagen (Aalborg University Copenhagen) and the other in Esbjerg (Aalborg University Esbjerg).\n· He is a member of prestigious IEEE (Institute of Electrical and Electronics Engineers), and IAENG (International Association of Engineers) organizations. \n· He is the chief Editor of the Journal of Software Engineering.\n· He is the member of the Editorial Board of International Journal of Computer Science and Software Technology (IJCSST) and International Journal of Computer Engineering and Information Technology. \n· He is also the Editor of Communication in Computer and Information Science CCIS-20 by Springer.\n· Reviewer For Many Conferences\nHe is the lead person in making collaboration agreements between Aalborg University and many universities of Pakistan, for which the MOU’s (Memorandum of Understanding) have been signed.\nProfessor Akbar is working in Academia since 1990, he started his career as a Lab demonstrator/TA at the University of Sussex. After finishing his P. hD degree in 1992, he served in the Industry as a Scientific Officer and continued his academic career as a visiting scholar for a number of educational institutions. In 1996 he joined National University of Science & Technology Pakistan (NUST) as an Associate Professor; NUST is one of the top few universities in Pakistan. In 1999 he joined an International Company Lineo Inc, Canada as Manager Compiler Group, where he headed the group for developing Compiler Tool Chain and Porting of Operating Systems for the BLACKfin processor. The processor development was a joint venture by Intel and Analog Devices. In 2002 Lineo Inc., was taken over by another company, so he joined Aalborg University Denmark as an Assistant Professor.\nProfessor Akbar has truly a multi-disciplined career and he continued his legacy and making progress in many areas of his interests both in teaching and research. 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He is currently a full professor in\nthe Department of Automation and Applied Informatics at the\nsame university. Dr. Voloşencu is the author of ten books, seven\nbook chapters, and more than 160 papers published in journals\nand conference proceedings. He has also edited twelve books and\nhas twenty-seven patents to his name. He is a manager of research grants, editor in\nchief and member of international journal editorial boards, a former plenary speaker, a member of scientific committees, and chair at international conferences. His\nresearch is in the fields of control systems, control of electric drives, fuzzy control\nsystems, neural network applications, fault detection and diagnosis, sensor network\napplications, monitoring of distributed parameter systems, and power ultrasound\napplications. 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