A comparison between culture and colony count, advanced molecular, and novel biosensors-based bacterial screening approaches, adapted and modified from [5].
\r\n\tThe book also covers the more specialized areas of energy consumption, riding comfort, noise and vibration.
\r\n\tEscalators and passengers conveyors should also be addressed, as these devices complement elevator system in moving passenger around the building.
\r\n\tModern developments are hope to be covered within the relevant chapters, some of which are listed as follows: Modern electrical safety systems,Modern shaft and motor feedback devices, Modern electrical drive system, Two elevator cars in the same shaft, Multiple elevator car systems in the same shaft, Evacuation systems using elevators, Modern calculation and simulation tools and software packages, Ropeless elevator systems.
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Rath",authors:[{id:"59158",title:"Dr.",name:"Eitan Z.",middleName:null,surname:"Rath",fullName:"Eitan Z. Rath",slug:"eitan-z.-rath"}]},{id:"23842",title:"Steroid Induced Glaucoma",slug:"steroid-induced-glaucoma",signatures:"Avraham Cohen",authors:[{id:"61596",title:"Dr.",name:"Avraham",middleName:null,surname:"Cohen",fullName:"Avraham Cohen",slug:"avraham-cohen"}]},{id:"23843",title:"Glaucoma in Cases of Penetrating Keratoplasty, Lamellar Procedures and Keratoprosthesis",slug:"glaucoma-in-cases-of-penetrating-keratoplasty-lamellar-procedures-and-keratoprosthesis",signatures:"Shimon Rumelt",authors:[{id:"54335",title:"Dr.",name:"Shimon",middleName:null,surname:"Rumelt",fullName:"Shimon Rumelt",slug:"shimon-rumelt"}]}]}],publishedBooks:[{type:"book",id:"237",title:"Astigmatism",subtitle:"Optics, Physiology and Management",isOpenForSubmission:!1,hash:"899b6b3d1d0250ee904841f62bac8bee",slug:"astigmatism-optics-physiology-and-management",bookSignature:"Michael Goggin",coverURL:"https://cdn.intechopen.com/books/images_new/237.jpg",editedByType:"Edited by",editors:[{id:"65903",title:"Dr.",name:"Michael",surname:"Goggin",slug:"michael-goggin",fullName:"Michael Goggin"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"268",title:"Glaucoma",subtitle:"Basic and Clinical Concepts",isOpenForSubmission:!1,hash:"b9a66374f7429cc798c56e9e8149a1aa",slug:"glaucoma-basic-and-clinical-concepts",bookSignature:"Shimon Rumelt",coverURL:"https://cdn.intechopen.com/books/images_new/268.jpg",editedByType:"Edited by",editors:[{id:"54335",title:"Dr.",name:"Shimon",surname:"Rumelt",slug:"shimon-rumelt",fullName:"Shimon Rumelt"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"286",title:"Keratoplasties",subtitle:"Surgical techniques and complications",isOpenForSubmission:!1,hash:"8e8d2ab941ea6074e7a7ae55de9c78fa",slug:"keratoplasties-surgical-techniques-and-complications",bookSignature:"Luigi Mosca",coverURL:"https://cdn.intechopen.com/books/images_new/286.jpg",editedByType:"Edited by",editors:[{id:"37700",title:"Dr.",name:"Luigi",surname:"Mosca",slug:"luigi-mosca",fullName:"Luigi Mosca"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"360",title:"Electroretinograms",subtitle:null,isOpenForSubmission:!1,hash:"bfbc5538173f11acb0f9549a85b70488",slug:"electroretinograms",bookSignature:"Gregor Belusic",coverURL:"https://cdn.intechopen.com/books/images_new/360.jpg",editedByType:"Edited by",editors:[{id:"44595",title:"Dr.",name:"Gregor",surname:"Belusic",slug:"gregor-belusic",fullName:"Gregor Belusic"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"540",title:"Age Related Macular Degeneration",subtitle:"The Recent Advances in Basic Research and Clinical Care",isOpenForSubmission:!1,hash:"457bf890ed1e947a2ed23fc8237e34f0",slug:"age-related-macular-degeneration-the-recent-advances-in-basic-research-and-clinical-care",bookSignature:"Gui-Shuang Ying",coverURL:"https://cdn.intechopen.com/books/images_new/540.jpg",editedByType:"Edited by",editors:[{id:"86603",title:"Dr.",name:"Gui-Shuang",surname:"Ying",slug:"gui-shuang-ying",fullName:"Gui-Shuang Ying"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}}],publishedBooksByAuthor:[{type:"book",id:"237",title:"Astigmatism",subtitle:"Optics, Physiology and Management",isOpenForSubmission:!1,hash:"899b6b3d1d0250ee904841f62bac8bee",slug:"astigmatism-optics-physiology-and-management",bookSignature:"Michael Goggin",coverURL:"https://cdn.intechopen.com/books/images_new/237.jpg",editedByType:"Edited by",editors:[{id:"65903",title:"Dr.",name:"Michael",surname:"Goggin",slug:"michael-goggin",fullName:"Michael Goggin"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}}]},onlineFirst:{chapter:{type:"chapter",id:"68700",title:"Principle and Development of Phage-Based Biosensors",doi:"10.5772/intechopen.86419",slug:"principle-and-development-of-phage-based-biosensors",body:'The risks due to bacterial contamination and infection to healthcare system and socio-economic stability as well as to environment and food contamination have become global issues [1]. The current approaches are usually not performing well in complex mixtures of opposing microorganisms and environmental conditions devoid of enrichment step. These approaches comprise old-fashioned plating and antibodies-based assays. Therefore, in the skipping of enrichment step, almost all present experiments are not satisfactorily sensitive to sense a distinct or a very small quantity of target bacteria [2]. In contrast, the approaches like hybridization-based assays (ELISA) and polymerase chain reaction (PCR) are sensitive; however, these cannot differentiate the live cells from the dead ones, thus require an augmentation step for specificity and are laborious and expensive. These restrictions can be potentially overwhelmed by developing a biosensor. Biosensor development needs a specific and sensitive bio-probe that can withstand elevated temperature, extreme pH and remain active in diverse and complicated environment. Bacteriophages being sensitive and specific to host bacterium, and showing activity in diverse ionic concentrations are potent agents in biosensor development for detection of bacteria. Phages naturally deliver specificity in recognition of particular bacterial strain to attach, and specifically sense preferred bacterial spectra. Swift recognition offered by phage-based detection can improve the tracing and remediation of bacterial contamination [3]. The main issue that comes with development of phage based biosensor is active and oriented phages immobilization on substrate surface. The benefit of phage immobilization during biosensor development is that phages remain active for long time period, retain physiological activities with high densities, and having high bacterial cells capture efficiencies. Thus, showing improved detection limits that leads to possible development of phage-based biosensor for rapid and accurate bacterial detection [4]. Bacteriophage based biosensor development involve the following phage related approaches: (i) Observing the released phage particles during lytic cycle in the presence of host bacterium, (ii) monitoring released intracellular lysed cell component in the course of phage-mediated bacterial lysis, (iii) detection of inhibited bacterial growth in the presence of specific phages, (iv) use of stained phages for bacterial capture, and (v) observing the expression of cloned reporter gene in genetically modified phages that is expressed after bacterial infection [5].
The conventional bacterial detection techniques such as colony count, bio-chemical and immunological procedures (ELISA) [6], and the modern (PCR) [7] approaches are currently widely in use; however, these approaches are time consuming as these need enrichment step. Consequently, there is a need to develop rapid and sensitive detecting methods. To this end, the use of biosensor, which can sense bacteria at diverse concentrations, are considered well applicable platform owing to their low cost, simplicity, and sensitivity [5]. Figure 1 shows different bacterial detection approaches and Table 1 summarizes comparative study of different bacterial detection methods.
Representation of various bacterial detection approaches.
Bacterial detection method | Personals | Cost and detection time | Tools | Live and dead cells detection | On spot detection | Ref. |
---|---|---|---|---|---|---|
Culture & colony count | Trained users, laborious | Cheap, 5–7 days | Simple | Yes | No | [2, 13, 14] |
PCR | Trained users, laborious, | Costly, 1–4 h | Specialized | No | No | |
ELISA | Trained users, laborious, | Costly, approx.: 4 h | High-tech | No | No | |
Nucleic acids-based-biosensor | Simple, automatic | Expensive, 0.5–2 h | Simple | No | Yes | [2, 15, 16] |
Antibodies-based-biosensor | Simple, automatic | Very expensive, 0.5–2 h | Simple | No | Yes | |
Phage-based-biosensor | Simple, Automatic | Cheap, 0.5–2 h | Simple | Yes | Yes |
A comparison between culture and colony count, advanced molecular, and novel biosensors-based bacterial screening approaches, adapted and modified from [5].
In such methods, bacteria existing in a sample are cultured on different types of media so that to confirm their existence and isolate them. Two main culturing approaches are used, quantitative and qualitative. By qualitative culturing technique, the target bacterial colonies are produced on selective or differential media. In quantitative culturing technique, the specific bacteria are propagated to form specific colonies which can be calculated to evaluate the sum of microorganisms. Finally, different biochemical tests are performed [8].
Immunological approaches, such as ELISA, depend upon the reaction of an antigen with a particular specific antibody. This method is unable to differentiate among living and dead cells and also antibodies production is very expensive [6].
Molecular procedures involve the use of DNA for the detection of target bacteria. For example, PCR, first pronounced in 1980s, is nowadays frequently used for detection of bacteria [7]. Molecular approaches are popular for their high sensitivity and rapidity. Dedicated apparatuses, skilled operators and expensive nature mark their rejection.
According to the proposed definition of biosensor by IUPAC, “Biosensor is a self-controlled imitated device, that is comprise a bio-recognition constituent (bio-prob/bio-receptor), connected to a transducer to translate the biological signal into a computer readable signal and is then presented on computer and analyzed [9] (Figure 2). The bio-probes used in general are bacteriophage, enzyme, whole cell, nucleic acid and antibody. The transducer is electrochemical, optical, or mass based, or combination of these. Typical features of biosensors include; selectivity, reproducibility, detection limit, stability, biocompatibility, sensitivity and linearity [10]. Biosensors are commonly used in medical, diagnostic, quality control, veterinary, food and dairy industry, viral and bacterial diagnostic, agriculture industry, drug production, mining, industrial waste water control, defense and military [11]. Classification of biosensor is based on the recognition element, that is, bio-probe (bacteriophage, enzyme, whole cell, nucleic acid and antibody) used or the type of transducer (electrical, optical, or thermal signals etc.) involved. A representative biosensor is comprised of analyte (target to be sensed), bio-receptor (bio-molecule that identifies the analyte), transducer (responsible for signal transduction) and electronics (display the transduced signal) [5].
Schematics representation of a generalized biosensors, reframed from [
As mentioned earlier, biosensor involves some biological recognition elements like bacteriophages [17], enzyme [18], whole cell [19], nucleic acid [20], and antibody [21], etc. These common bio-probe are briefed in the following sections:
To accomplish the requisite for up-to-date and fast bio-sensing schemes, antibodies (Abs) have become important affinity ligands to detect pathogens in clinical and food samples. Definitely, Abs when immobilized on a surface, these interact with specific antigens present on microbial surfaces, thus inducing a computable signal by an output detector. Abs popularity ascends from numerous benefits, for example, adaptability, ease of incorporation into diverse systems and are highly specific to their target antigens [21].
From the time of first biosensor (glucose sensor by Clark and Lyons in 1962), enzyme-based biosensors have shown immense progress in many applications. Enzymes are precise competent bioanalytical agents, having the ability to precisely mark out their substrates. This distinctive property mark enzymes potent implements in the development of analytical devices [18]. These biosensors company closely a biocatalyst-comprising a detecting coating with a transducer. Its operational principal is based on the catalysis and binding abilities for specific detection.
The sequence of nucleic acids for a precise detection was established in 1953 and is still developing widely [20]. These biosensors involve nucleic acids as a bio-recognition-prob. The high specific binding between the two single strands of DNA (ssDNA) sequences to make double stranded DNA (dsDNA) sequence is used to develop nucleic acids-based biosensor. This technique validated to develop DNA-built-biosensor from the old-style technique like pairing of radio iso-tropic and electrophoretic separations that are costly, dangerous, and time consuming.
These biosensors involve living cells as a bio-probe and detecting component. They are constructed on the basis of living cell ability to sense the physiological parameters, and the extracellular and intracellular micro-environmental conditions, and as a result a response is produced by the reaction between cell and stimulus [19]. Microbial cells, for example fungi and bacteria are commonly used to develop whole cell based biosensors to sense particular molecules or the inclusive “condition” of the nearby environs.
Phages are virus particles, infecting and reproducing only within bacterial cells. Because of their associated evolution along with bacteria, phages have extremely specific machineries to identify and then infect their host bacteria for propagation. Phages generally have two distinctive chunks, the head comprising genetic material while the tail accountable to recognize and attach to bacterial cell [22]. Phages have several biomedical applications, and owing to their specificity they are extensively used for specific and sensitive detection of bacteria [23]. Most significant feature of phages is that they can only identify, and attack living bacterial cells. This exciting feature was well demonstrated by Fernandes et al., to detect viable, viable but not culture-able, or totally dead
A phage as a bio-recognition probe offers numerous benefits in rapid bacterial sensing [17] as they are: (•) extremely specific to their host [26], (•) ability of producing extraordinary titers of descendant phages, (•) tolerant to extreme environmental conditions like ultrahigh temperatures, organic solvents and wide-ranging pH compared to Abs, [27], (•) safe handling, (•) discriminating among dead and live bacteria as they proliferate only in live bacterial cells [28], (•) production in bulk are artless and economical. These compensations make phages as leading bio-recognition probes to develop biosensors for bacterial screening [15]. Frequently designed phage-based biosensor schemes comprise the association of whole phage or phage-constituents, infecting/capturing target bacterial cells and ultimately resulting in the production of electrical, colorimetric, fluorescent, or luminescent etc. signals, based on the available biosensing system. Hence, phages are demonstrating themselves as novel troupes for cheap, fast, sensitive and specific bacterial detection in comparison to other available platforms [29].
Reporter bacteriophages are genetically edited phages used to import and insert a specific gene into the genome of target bacteria. The foreign gene inserted to host bacteria is expressed, bacteria are marked based on available platforms as a colorimetric, optical, or as a fluorescent marker and thus bacterial screening is permitted [30]. Irrespective of, whether reporter bacteriophages are lysogenic or lytic, both can detect potentially the particular pathogenic bacteria. A number of genes, such as insertion of firefly
Phages stained with different fluorescent dyes have been used for target bacterial detection involving various fluorescence sensing tools. Stained phage-probes can discriminate a target bacterium when they infect and attack host cells [33]. Like, phages were tagged with fluorescent quantum dots (QDs) and
Lytic phages infection results in cell burst and consequently intracellular organelles, descendant phages, and cell-lysis materials are released. Both the release of intracellular elements and released progeny phages provide a base to recognize the target bacterium [30]. For example, as a released cell component, adenosine-triphosphate can be detected through bioluminescence just after target bacterial cell lysis [35]. Also the amount of released progeny phages released after cell lysis by a particular phage is directly proportional to the amount of lysed cells and can be used for bacterial sensing [36]. The released progeny phages enumerated by various detection mechanisms such as plaque- or immuno assays, molecular methods like quantitative PCR (qPCR) and, or by isothermal nucleic acid amplification (ITNAA) [37].
Phages that are immobilized on solid matrix can be utilized for capturing specific bacterial cell from contained samples. Bacteriophages have a many functionally active groups like hydroxyl group (—OH), aldehyde group(—CHO), carboxyl group(—COOH), etc., on their exteriors, giving them inimitable characteristics permitting their interaction with other materials and to interact with bacterial surface receptor molecules [38]. Consequently phages have been successfully used to capture particular bacterial cells from different samples [39, 40]. Like streptavidin actuated gold nano-particles were used to immobilize GM T4 bacteriophage particles. Delay in impedance was observed due to bacterial cells binding that marked as a sign for the existence of bacterial cells [41].
Some phage components display natural magnetism to host cell for example receptor-binding proteins (RBPs), but they are highly subtle to variations in environmental conditions. Phage tail bears RBPs and helps in binding to host bacterium, proceeding to insert its genetic material within the cell and cell infection is established [42]. RPBs bind to cell surface with help of specific polypeptide or polysaccharide sequences that are present on the cell surface. Poshtiban and colleagues activated magnetic beads by immobilization of RBP protein Gp047 (from phage NCTC12673). These functionally active beads were then utilized for
It’s obvious from the literature that different approaches have been developed for immobilization of phages on surface of electrodes Figure 3. The common phage immobilization strategies on solid surfaces include physical adsorption [44], covalent bonding [45], entrapment of phages in solid matrix [46], etc.
Different ways to potentially orient phages on solid surfaces. Green highlighted-bacterial binding proteins, (a) tailed phages-side-ways, head-down, or tail-down, (b) asymmetric icosahedral phages, (c) filamentous phages-through either side-ways or, pole, (d) filamentous phages are likely to be bundled or aggregated (left). Oriented typically parallel on the substrate (right), adapted from [
The quantity of randomly oriented phages on solid surfaces is the most straightforward way for enhancing signal in bio-sensing scheme [47]. Deposition of high number of phage particles creates a steric interruption between phage particles [39]. Thus number of phage particles immobilized on solid surface should not surpass a specific threshold per surface area [48]. For T4 phage, estimated optical density was 19 phages/ mm2 area beyond that clogging was happened, resulting in reduced signal [49]. Phage particles can be simply oriented on the surface of electrode as they bear positive and negative potential on their tail fibers and head respectively. Phage immobilization strategies are briefly highlighted in the following context.
Most common approach used for immobilization of phages is physisorption [50, 51]. This approach is very artless, but then again the adsorbed phage may possibly detach as of substrate surface because of shear, changes in pH, or temperature, or ionic concentrations caused in the medium that reduces principally their biosensing applications. Subsequently most phage particles having net negative charge at pH 7 [52], a number of investigators successfully used electrostatic binding for phages immobilization Figure 4, [52]. Also this methodology suffers due to variability and bacteriophage detachment in turn to the physico-chemical fluctuations in the analyte medium. Covalent bonding of phages offered a more stronger attachment and is not at risk to easy detachment of phages [53, 54]. Proper chemical studies can make easy selection of suitable substrate and then potential application. Covalent attachment resulted in a sophisticated bacteriophage surface mass that is principally necessary for phage application in biosensor development [55, 56]. To design bioactive surfaces with phages, phage infectivity is important or at least phage should be able to interact with host bacteria or analyte; therefore, optimization is needed to reduce the effect on bacteriophage integrity during immobilization.
Graphical representation of bacteriophage random immobilization and electrostatic, charge-directed orientated immobilization of T2 phage onto CNT electrode surface functionalized with polyethyleneimine (PEI) [
Bacteriophage-display tools can enable scientist to display peptides of choice present on the phage exterior, that is, phage envelope. Phages expressed peptide can consequently be adsorbed on material surfaces that are coated with peptides specific ligands Figure 5. Phage-display-libraries are produced by introducing DNA segments into specific phages to facilitate each phage to display a specific peptide expressed by the DNA segment inserted [58]. Technology of phage display developed as a combined influence of two central thoughts, fusion phage and combinatorial peptide libraries [59]. The first theory allows display of external peptides on bacteriophage surface [59]; while the second idea hires libraries of numerous peptides achieved in corresponding production as contrasting to production of single specific peptides [60]. Merging these two theories stemmed progress in phage-display-tools, multi-billion clone alignments of self-assembled and self-amplified bio-components [54]. It is significant to keep in mind that genetic alteration may alter the characteristics properties of bacteriophages. For example, biotin-carboxyl-carrier-protein (BCCP) gene or the cellulose-binding-module (CBM) gene to the small-outer-capsid-protein (SOCP) gene of T4 bacteriophage was attached, affecting bacteriophage infectivity, and result was decline in burst size, as well as extended latent period [61].
Current applications of phage display technologies as imaging agents. Icosahedral phages are mostly used as, aiming on moieties for bacterial detection, and substrates for signal amplification. While filamentous phages are mostly used as multifunctional probes, and a variety of sensors [
Bacteriophages immobilization in micro-porous matrices permits them functionally and also structurally stable, keeping them active for long time period. Phages immobilization by entrapment in a porous hydrogel, (bio)polymeric agar and alginate matrices, is a tool for selection of applications where protection of phage particles essential against severe environmental conditions [62]. Additionally, entrapment might aid to maintain moisture, which is important for many phages infectivity, or keep phage particles in lyophilized condition [63]. A fruitful marketable case in point of entrapped bacteriophage in matrix is PhagoBioDerm [64] that is 0.2 mm thick, porous-polymeric-wound-dressing saturated with a mixture of biocides and lytic phages [65]. The matrices used for bacteriophage entrapment, that might possibly delay interaction of entrapped bacteriophage particles with host bacterial cells or analytes that are present in the vicinity of medium [66], marking inefficiency of phage bioactive surface.
Many investigators discovered to possibly immobilize phages by alternative layering with polyelectrolytes having oppositely charges, and claimed observation of enhanced phage particle surface coverage [68, 69]. For instance, a layer by layer methodology for M13 bacteriophage was reported, and phage was sandwiched between oppositely charged layers of weak poly-electrolytes, that was capable to diffuse freely form a nearby packed phage monolayer [69].
The effectiveness of bio-sensing approaches is mostly measured in terms of minimum limit of detection (LOD) of bacterial or other analyte. Thus researchers attempted and focused to improve the bacteriophage surface coverage for pushing detection limits. Significantly keep in mind that the LOD has not been improved biosensors where phages are immobilized by covalent binding, in comparison to the approaches where phage is immobilized by physisorption [22]. Thus, bacteriophage surface coverage is not only the factor to necessarily increase and improve the sensitivity and LOD of bacteriophage-based biosensor. Limit of detection of biosensors, based on various transduction approaches can be different depending on the working principle of selected transduction platform.
Without any doubt, environmental monitoring and food safety are the main universal worries that we humans have to oppose and are constantly struggling to take them over. In this chapter, we evidently demonstrated the principle and development phage-based biosensor. We compared the conventional phage based detection methods and briefed an introduction to different bio-probes involved in biosensors development. Further, we reviewed demonstrative phage/phage-components used in sensors development for pathogenic bacterial detection. Finally, we briefed different techniques to immobilize phages on appropriate substrate that is the major step toward phage-based biosensor development. We intend at thought-provoking and comprehensive explanations in mounting phage-based sensors and enlightening their uses for bacterial detection. By collaboration of engineers and scientists from multidisciplinary area to design a field applicable sensor and make advancements in phage-based sensors for bacterial pathogens diagnosis, we expect that this chapter might bring together the technologies related to phage-based sensors. In short, phage based biosensors in the fields of food safety, environmental monitoring and infectious disease diagnostics is vital as they are;
Cheap (based on easy phages production)
Highly specific
Very sensitive
Versatile (based on phage components)
This work was supported by National Key Research and Development Program of China under Grant 2017YFC1104402, China Postdoctoral Science Foundation (2016 M602291), the initial Research fund from CSC; and 3551 Project, Optics Valley of China.
antibodies enzyme linked immunosorbent assay isothermal nucleic acid amplification International Union of Pure and Applied Chemistry limit of detection polymerase chain reaction quantum dots quantitative polymerase chain reaction receptor-binding proteins biotin-carboxyl-carrier-protein cellulose-binding-module small-outer-capsid-protein
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This chapter aims to discuss the main mechanisms of the interaction between PGPR and plants and will focus of how PGPR can decrease abiotic stress damage in cereals, which are critical crops for human diet.",book:{id:"8044",slug:"root-biology-growth-physiology-and-functions",title:"Root Biology",fullTitle:"Root Biology - Growth, Physiology, and Functions"},signatures:"Martino Schillaci, Sneha Gupta, Robert Walker and Ute Roessner",authors:null},{id:"64176",doi:"10.5772/intechopen.81186",title:"Tropical Forage Legumes in India: Status and Scope for Sustaining Livestock Production",slug:"tropical-forage-legumes-in-india-status-and-scope-for-sustaining-livestock-production",totalDownloads:1997,totalCrossrefCites:3,totalDimensionsCites:5,abstract:"Livestock contributes enormously in food and nutritional security apart from livelihood security to rural population all over the world. India has the largest number of livestock, representing over 17% of world population. Availability of forage legumes is essential for better animal health, production and increasing the nutritive value of forage-based rations, besides providing a source of biological nitrogen fixation for enriching soil, reducing land degradation and mitigating climate change. However, supply of quality green fodder in India is extremely precarious, and the gap is huge against demand. The major fodder legume crops cultivated in India are Medicago sativa, Trifolium alexandrinum, Vigna unguiculata, Vigna umbellate and range legumes are Stylosanthes spp., Desmanthus virgatus, and Clitoria ternatea. Indian subcontinent represents wide spectrum of eco-climates and reported diversity of 21 forage legumes genera viz., Desmodium, Lablab, Stylosanthes, Vigna, Macroptelium, Centrosema and browse plants Leucaena, Sesbania, Albizia, Bauhinia, Cassia, Grewia, etc. Diversity of forage legumes were collected (>3200 accessions), evaluated and sources for different biotic and abiotic stress tolerance were identified, apart from >50 cultivars developed. Considering these aspects, tropical legumes for livestock production, soil health and ecosystem services, diversity, evaluation and breeding for improved varieties are discussed in this chapter.",book:{id:"7491",slug:"forage-groups",title:"Forage Groups",fullTitle:"Forage Groups"},signatures:"Tejveer Singh, Srinivasan Ramakrishnan, Sanat Kumar Mahanta,\nVikas C. Tyagi and Ajoy Kumar Roy",authors:[{id:"254954",title:"Dr.",name:"Srinivasan",middleName:null,surname:"Ramakrishnan",slug:"srinivasan-ramakrishnan",fullName:"Srinivasan Ramakrishnan"},{id:"254958",title:"Dr.",name:"Tejveer",middleName:null,surname:"Singh",slug:"tejveer-singh",fullName:"Tejveer Singh"},{id:"254959",title:"Dr.",name:"Sanath Kumar",middleName:null,surname:"Mahanta",slug:"sanath-kumar-mahanta",fullName:"Sanath Kumar Mahanta"},{id:"254960",title:"Dr.",name:"Vikas",middleName:null,surname:"Tyagi",slug:"vikas-tyagi",fullName:"Vikas Tyagi"},{id:"254961",title:"Dr.",name:"A.K.",middleName:null,surname:"Roy",slug:"a.k.-roy",fullName:"A.K. Roy"}]},{id:"65365",doi:"10.5772/intechopen.83402",title:"Mob Grazing Results in High Forage Utilization and Reduced Western Snowberry Size",slug:"mob-grazing-results-in-high-forage-utilization-and-reduced-western-snowberry-size",totalDownloads:950,totalCrossrefCites:0,totalDimensionsCites:3,abstract:"Mob-grazing strives to maximize forage utilization and minimize selective grazing by using high stocking densities in small paddocks for short durations (12–24 hr). Rotational-grazing uses low stocking densities for a longer time period, retaining about half of the original available forage; although selective grazing can occur. Three cattle (Bos taurus × Bos indicus) grazing intensities: mob- (stocking densities from 32,000 to 67,000 kg ha−1; duration—24 hr); rotation (stocking density—2500 kg ha−1; duration—35 d); and non-grazed systems were compared based on forage utilization and changes to western snowberry (Symphoricarpos occidentalis) (WS) patch volume in a 2-year South Dakota study. Pre- and post-grazing forage height was measured every 2.5 m along multiple 50-m transects with WS patch volume measured every 5 m. Forage utilization (consumed and trampled) ranged from 42 to 90% in mob-grazed areas, and harvest efficiency (forage consumed) ranged from 15 to 64%. WS patch volumes decreased by ≥45% in mob-grazed treatments compared with no change in rotational-grazing and increased cover in non-grazed areas. WS pre-graze patch size influenced mob-grazing impact; patches >6500 cm3 were browsed or trampled to a greater extent than smaller patches.",book:{id:"7491",slug:"forage-groups",title:"Forage Groups",fullTitle:"Forage Groups"},signatures:"Heidi Reed, Sharon Clay, Alexander Smart, David Clay and Michelle\nOhrtman",authors:[{id:"37140",title:"Prof.",name:"David",middleName:null,surname:"Clay",slug:"david-clay",fullName:"David Clay"},{id:"87430",title:"Prof.",name:"Sharon",middleName:null,surname:"Clay",slug:"sharon-clay",fullName:"Sharon Clay"},{id:"255998",title:"Dr.",name:"Heidi",middleName:null,surname:"Reed",slug:"heidi-reed",fullName:"Heidi Reed"},{id:"256000",title:"Dr.",name:"Alexander",middleName:null,surname:"Smart",slug:"alexander-smart",fullName:"Alexander Smart"},{id:"285225",title:"Dr.",name:"Michelle",middleName:null,surname:"Ohrtman",slug:"michelle-ohrtman",fullName:"Michelle Ohrtman"}]},{id:"65604",doi:"10.5772/intechopen.83643",title:"Evaluation and Prediction of the Nutritive Value of Underutilised Forages as Potential Feeds for Ruminants",slug:"evaluation-and-prediction-of-the-nutritive-value-of-underutilised-forages-as-potential-feeds-for-rum",totalDownloads:1539,totalCrossrefCites:0,totalDimensionsCites:2,abstract:"The aim of the chapter was to evaluate and predict the nutritive and feeding value of unknown and underutilised forages. Underutilised forages were collected from various regions. Chemical composition and degradability of forages in the rumen were determined. A dataset was created bearing degradability parameters of feeds from 40 studies. Using the dataset, a step-wise regression procedure was used to develop regression equations to predict rumen degradability. Of the underutilised forages, crude protein content tended to be double for Brassica oleracea var. acephala compared to Colophospermum mopane leaves and pods. Forage grasses tended to have very low crude protein contents compared to legumes and concentrates. Underutilised Brassica oleracea var. acephala tended to have higher crude protein levels compared to commonly used protein sources. The regression model for predicting the soluble fraction accounted for 59% (development) and 71% (validation) of the variation. The regression model for predicting the potential degradability accounted for 65% (development) and 24% (validation) of the variation. In conclusion, the nutritive value of underutilised forages was good, high in crude protein and high potential degradability. After correcting for factors that significantly affected degradability parameters, predicted solubility and effective degradability lay near the ideal prediction line, giving good predictions.",book:{id:"7491",slug:"forage-groups",title:"Forage Groups",fullTitle:"Forage Groups"},signatures:"Mehluli Moyo, Siyabonga T. Bhiya, Masande Katamzi and Ignatius\nV. Nsahlai",authors:[{id:"201527",title:"Prof.",name:"Ignatius V.",middleName:null,surname:"Nsahlai",slug:"ignatius-v.-nsahlai",fullName:"Ignatius V. Nsahlai"},{id:"203798",title:"Mr.",name:"Mehluli",middleName:null,surname:"Moyo",slug:"mehluli-moyo",fullName:"Mehluli Moyo"},{id:"260135",title:"Mr.",name:"Katamzi",middleName:null,surname:"Masande",slug:"katamzi-masande",fullName:"Katamzi Masande"},{id:"283732",title:"Mr.",name:"Siyabonga",middleName:null,surname:"Bhiya",slug:"siyabonga-bhiya",fullName:"Siyabonga Bhiya"}]},{id:"67736",doi:"10.5772/intechopen.86996",title:"The Infection Unit: An Overlooked Conceptual Unit for Arbuscular Mycorrhizal Function",slug:"the-infection-unit-an-overlooked-conceptual-unit-for-arbuscular-mycorrhizal-function",totalDownloads:935,totalCrossrefCites:2,totalDimensionsCites:2,abstract:"Most land plant species have their roots colonized by arbuscular mycorrhizal fungi (AMF). These symbiotic associations are often found in the roots of field crops. The biological basis and practical significance of this symbiosis have been extensively studied, and the molecular mechanisms underlying the initial colonization process and the nutrient exchange between the host plant and the AMF have been elucidated. However, developmental processes and turnover of elements of the mycorrhiza, and the resulting changes in mycorrhizal function, are not well understood. The enigmatic nature of the development-function relationship is probably due to the short life span of the infection unit, which has largely been overlooked in studies investigating mycorrhizal function at the macroscopic level. This paper outlines the concept of the infection unit and functional expression patterns in terms of the transient aspects of the micro-symbiont during its life cycle in this symbiosis.",book:{id:"8044",slug:"root-biology-growth-physiology-and-functions",title:"Root Biology",fullTitle:"Root Biology - Growth, Physiology, and Functions"},signatures:"Yoshihiro Kobae",authors:null}],mostDownloadedChaptersLast30Days:[{id:"63148",title:"Domestic Livestock and Its Alleged Role in Climate Change",slug:"domestic-livestock-and-its-alleged-role-in-climate-change",totalDownloads:15845,totalCrossrefCites:0,totalDimensionsCites:0,abstract:"It is very old wisdom that climate dictates farm management strategies. In recent years, however, we are increasingly confronted with claims that agriculture, livestock husbandry, and even food consumption habits are forcing the climate to change. We subjected this worrisome concern expressed by public institutions, the media, policy makers, and even scientists to a rigorous review, cross-checking critical coherence and (in)compatibilities within and between published scientific papers. Our key conclusion is there is no need for anthropogenic emissions of greenhouse gases (GHGs), and even less so for livestock-born emissions, to explain climate change. Climate has always been changing, and even the present warming is most likely driven by natural factors. The warming potential of anthropogenic GHG emissions has been exaggerated, and the beneficial impacts of manmade CO2 emissions for nature, agriculture, and global food security have been systematically suppressed, ignored, or at least downplayed by the IPCC (Intergovernmental Panel on Climate Change) and other UN (United Nations) agencies. Furthermore, we expose important methodological deficiencies in IPCC and FAO (Food Agriculture Organization) instructions and applications for the quantification of the manmade part of non-CO2-GHG emissions from agro-ecosystems. However, so far, these fatal errors inexorably propagated through scientific literature. Finally, we could not find a clear domestic livestock fingerprint, neither in the geographical methane distribution nor in the historical evolution of mean atmospheric methane concentration. In conclusion, everybody is free to choose a vegetarian or vegan lifestyle, but there is no scientific basis, whatsoever, for claiming this decision could contribute to save the planet’s climate.",book:{id:"7491",slug:"forage-groups",title:"Forage Groups",fullTitle:"Forage Groups"},signatures:"Albrecht Glatzle",authors:[{id:"252990",title:"Dr.",name:"Albrecht",middleName:null,surname:"Glatzle",slug:"albrecht-glatzle",fullName:"Albrecht Glatzle"}]},{id:"32711",title:"Flooding Stress on Plants: Anatomical, Morphological and Physiological Responses",slug:"flooding-stress-on-plants-anatomical-morphological-and-physiological-responses",totalDownloads:7754,totalCrossrefCites:0,totalDimensionsCites:0,abstract:null,book:{id:"1814",slug:"botany",title:"Botany",fullTitle:"Botany"},signatures:"Gustavo Gabriel Striker",authors:[{id:"93232",title:"Prof.",name:"Gustavo",middleName:"Gabriel",surname:"Striker",slug:"gustavo-striker",fullName:"Gustavo Striker"}]},{id:"65604",title:"Evaluation and Prediction of the Nutritive Value of Underutilised Forages as Potential Feeds for Ruminants",slug:"evaluation-and-prediction-of-the-nutritive-value-of-underutilised-forages-as-potential-feeds-for-rum",totalDownloads:1537,totalCrossrefCites:0,totalDimensionsCites:2,abstract:"The aim of the chapter was to evaluate and predict the nutritive and feeding value of unknown and underutilised forages. Underutilised forages were collected from various regions. Chemical composition and degradability of forages in the rumen were determined. A dataset was created bearing degradability parameters of feeds from 40 studies. Using the dataset, a step-wise regression procedure was used to develop regression equations to predict rumen degradability. Of the underutilised forages, crude protein content tended to be double for Brassica oleracea var. acephala compared to Colophospermum mopane leaves and pods. Forage grasses tended to have very low crude protein contents compared to legumes and concentrates. Underutilised Brassica oleracea var. acephala tended to have higher crude protein levels compared to commonly used protein sources. The regression model for predicting the soluble fraction accounted for 59% (development) and 71% (validation) of the variation. The regression model for predicting the potential degradability accounted for 65% (development) and 24% (validation) of the variation. In conclusion, the nutritive value of underutilised forages was good, high in crude protein and high potential degradability. After correcting for factors that significantly affected degradability parameters, predicted solubility and effective degradability lay near the ideal prediction line, giving good predictions.",book:{id:"7491",slug:"forage-groups",title:"Forage Groups",fullTitle:"Forage Groups"},signatures:"Mehluli Moyo, Siyabonga T. Bhiya, Masande Katamzi and Ignatius\nV. Nsahlai",authors:[{id:"201527",title:"Prof.",name:"Ignatius V.",middleName:null,surname:"Nsahlai",slug:"ignatius-v.-nsahlai",fullName:"Ignatius V. Nsahlai"},{id:"203798",title:"Mr.",name:"Mehluli",middleName:null,surname:"Moyo",slug:"mehluli-moyo",fullName:"Mehluli Moyo"},{id:"260135",title:"Mr.",name:"Katamzi",middleName:null,surname:"Masande",slug:"katamzi-masande",fullName:"Katamzi Masande"},{id:"283732",title:"Mr.",name:"Siyabonga",middleName:null,surname:"Bhiya",slug:"siyabonga-bhiya",fullName:"Siyabonga Bhiya"}]},{id:"76675",title:"Introductory Chapter: Studies on Cucumber",slug:"introductory-chapter-studies-on-cucumber",totalDownloads:409,totalCrossrefCites:0,totalDimensionsCites:1,abstract:null,book:{id:"9704",slug:"cucumber-economic-values-and-its-cultivation-and-breeding",title:"Cucumber Economic Values and Its Cultivation and Breeding",fullTitle:"Cucumber Economic Values and Its Cultivation and Breeding"},signatures:"Huixia Jia and Haiping Wang",authors:[{id:"280406",title:"Dr.",name:"Haiping",middleName:null,surname:"Wang",slug:"haiping-wang",fullName:"Haiping Wang"},{id:"417904",title:"Dr.",name:"Huixia",middleName:null,surname:"Jia",slug:"huixia-jia",fullName:"Huixia Jia"}]},{id:"64176",title:"Tropical Forage Legumes in India: Status and Scope for Sustaining Livestock Production",slug:"tropical-forage-legumes-in-india-status-and-scope-for-sustaining-livestock-production",totalDownloads:1996,totalCrossrefCites:3,totalDimensionsCites:5,abstract:"Livestock contributes enormously in food and nutritional security apart from livelihood security to rural population all over the world. India has the largest number of livestock, representing over 17% of world population. Availability of forage legumes is essential for better animal health, production and increasing the nutritive value of forage-based rations, besides providing a source of biological nitrogen fixation for enriching soil, reducing land degradation and mitigating climate change. However, supply of quality green fodder in India is extremely precarious, and the gap is huge against demand. The major fodder legume crops cultivated in India are Medicago sativa, Trifolium alexandrinum, Vigna unguiculata, Vigna umbellate and range legumes are Stylosanthes spp., Desmanthus virgatus, and Clitoria ternatea. Indian subcontinent represents wide spectrum of eco-climates and reported diversity of 21 forage legumes genera viz., Desmodium, Lablab, Stylosanthes, Vigna, Macroptelium, Centrosema and browse plants Leucaena, Sesbania, Albizia, Bauhinia, Cassia, Grewia, etc. Diversity of forage legumes were collected (>3200 accessions), evaluated and sources for different biotic and abiotic stress tolerance were identified, apart from >50 cultivars developed. Considering these aspects, tropical legumes for livestock production, soil health and ecosystem services, diversity, evaluation and breeding for improved varieties are discussed in this chapter.",book:{id:"7491",slug:"forage-groups",title:"Forage Groups",fullTitle:"Forage Groups"},signatures:"Tejveer Singh, Srinivasan Ramakrishnan, Sanat Kumar Mahanta,\nVikas C. 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The whole process of submitting an article and editing of the submitted article goes extremely smooth and fast, the number of reads and downloads of chapters is high, and the contributions are also frequently cited.",author:{id:"55578",name:"Antonio",surname:"Jurado-Navas",institutionString:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRisIQAS/Profile_Picture_1626166543950",slug:"antonio-jurado-navas",institution:{id:"720",name:"University of Malaga",country:{id:null,name:"Spain"}}}}]},series:{item:{id:"13",title:"Veterinary Medicine and Science",doi:"10.5772/intechopen.73681",issn:"2632-0517",scope:"Paralleling similar advances in the medical field, astounding advances occurred in Veterinary Medicine and Science in recent decades. These advances have helped foster better support for animal health, more humane animal production, and a better understanding of the physiology of endangered species to improve the assisted reproductive technologies or the pathogenesis of certain diseases, where animals can be used as models for human diseases (like cancer, degenerative diseases or fertility), and even as a guarantee of public health. Bridging Human, Animal, and Environmental health, the holistic and integrative “One Health” concept intimately associates the developments within those fields, projecting its advancements into practice. This book series aims to tackle various animal-related medicine and sciences fields, providing thematic volumes consisting of high-quality significant research directed to researchers and postgraduates. It aims to give us a glimpse into the new accomplishments in the Veterinary Medicine and Science field. By addressing hot topics in veterinary sciences, we aim to gather authoritative texts within each issue of this series, providing in-depth overviews and analysis for graduates, academics, and practitioners and foreseeing a deeper understanding of the subject. Forthcoming texts, written and edited by experienced researchers from both industry and academia, will also discuss scientific challenges faced today in Veterinary Medicine and Science. 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After almost 32 years of teaching at the University of Trás-os-Montes and Alto Douro, she recently moved to the University of Évora, Department of Veterinary Medicine, where she teaches in the field of Animal Reproduction and Clinics. Her primary research areas include the molecular markers of the endometrial cycle and the embryo–maternal interaction, including oxidative stress and the reproductive physiology and disorders of sexual development, besides the molecular determinants of male and female fertility. She often supervises students preparing their master's or doctoral theses. 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Dr. Gaiceanu is a member of the National Council for Attesting Titles, Diplomas and Certificates, an expert of the Executive Agency for Higher Education, Research Funding, and a member of the Senate of the Dunarea de Jos University of Galati. He has been the head of the Integrated Energy Conversion Systems and Advanced Control of Complex Processes Research Center, Romania, since 2016. He has conducted several projects in power converter systems for electrical drives, power quality, PEM and SOFC fuel cell power converters for utilities, electric vehicles, and marine applications with the Department of Regulation and Control, SIEI S.pA. (2002–2004) and the Polytechnic University of Turin, Italy (2002–2004, 2006–2007). He is a member of the Institute of Electrical and Electronics Engineers (IEEE) and cofounder-member of the IEEE Power Electronics Romanian Chapter. He is a guest editor at Energies and an academic book editor for IntechOpen. 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He has more than 200 publications in reputed international journals, refereed conference proceedings, and 20 book chapters in books published by internationally renowned publishing houses, such as Springer, CRC press, IGI Global, etc. Currently, he is serving on the editorial board of the prestigious journal Frontiers in Communications and Networks and in the technical program committees of a number of high-ranked international conferences organized by the IEEE, USA, and the ACM, USA. 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Ms. Mehtab has published seven papers in international conferences and one of her papers has been accepted for publication in a reputable international journal. She has won the best paper awards in two prestigious international conferences – BAICONF 2019, and ICADCML 2021, organized in the Indian Institute of Management, Bangalore, India in December 2019, and SOA University, Bhubaneswar, India in January 2021. Besides, Ms. Mehtab has also published two book chapters in two books. Seven of her book chapters will be published in a volume shortly in 2021 by Cambridge Scholars’ Press, UK. Currently, she is working as the joint editor of two edited volumes on Time Series Analysis and Forecasting to be published in the first half of 2021 by an international house. Currently, she is working as a Data Scientist with an MNC in Delhi, India.",institutionString:"NSHM College of Management and Technology",institution:null},{id:"226240",title:"Dr.",name:"Andri Irfan",middleName:null,surname:"Rifai",slug:"andri-irfan-rifai",fullName:"Andri Irfan Rifai",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/226240/images/7412_n.jpg",biography:"Andri IRFAN is a Senior Lecturer of Civil Engineering and Planning. He completed the PhD at the Universitas Indonesia & Universidade do Minho with Sandwich Program Scholarship from the Directorate General of Higher Education and LPDP scholarship. He has been teaching for more than 19 years and much active to applied his knowledge in the project construction in Indonesia. His research interest ranges from pavement management system to advanced data mining techniques for transportation engineering. He has published more than 50 papers in journals and 2 books.",institutionString:null,institution:{name:"Universitas Internasional Batam",country:{name:"Indonesia"}}},{id:"314576",title:"Dr.",name:"Ibai",middleName:null,surname:"Laña",slug:"ibai-lana",fullName:"Ibai Laña",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/314576/images/system/314576.jpg",biography:"Dr. Ibai Laña works at TECNALIA as a data analyst. He received his Ph.D. in Artificial Intelligence from the University of the Basque Country (UPV/EHU), Spain, in 2018. He is currently a senior researcher at TECNALIA. His research interests fall within the intersection of intelligent transportation systems, machine learning, traffic data analysis, and data science. He has dealt with urban traffic forecasting problems, applying machine learning models and evolutionary algorithms. He has experience in origin-destination matrix estimation or point of interest and trajectory detection. Working with large volumes of data has given him a good command of big data processing tools and NoSQL databases. He has also been a visiting scholar at the Knowledge Engineering and Discovery Research Institute, Auckland University of Technology.",institutionString:"TECNALIA Research & Innovation",institution:{name:"Tecnalia",country:{name:"Spain"}}},{id:"314575",title:"Dr.",name:"Jesus",middleName:null,surname:"L. Lobo",slug:"jesus-l.-lobo",fullName:"Jesus L. Lobo",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/314575/images/system/314575.png",biography:"Dr. Jesús López is currently based in Bilbao (Spain) working at TECNALIA as Artificial Intelligence Research Scientist. In most cases, a project idea or a new research line needs to be investigated to see if it is good enough to take into production or to focus on it. That is exactly what he does, diving into Machine Learning algorithms and technologies to help TECNALIA to decide whether something is great in theory or will actually impact on the product or processes of its projects. So, he is expert at framing experiments, developing hypotheses, and proving whether they’re true or not, in order to investigate fundamental problems with a longer time horizon. He is also able to design and develop PoCs and system prototypes in simulation. He has participated in several national and internacional R&D projects.\n\nAs another relevant part of his everyday research work, he usually publishes his findings in reputed scientific refereed journals and international conferences, occasionally acting as reviewer and Programme Commitee member. Concretely, since 2018 he has published 9 JCR (8 Q1) journal papers, 9 conference papers (e.g. ECML PKDD 2021), and he has co-edited a book. 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He is an Associate Professor at the Department of Biomedical Engineering at Izmir Katip Celebi University, Izmir / Turkey, since 2019. In addition to academics, he has also founded Islerya Medical and Information Technologies Company, Izmir / Turkey, since 2017.\n\nHis main research interests cover biomedical signal processing, pattern recognition, medical device design, programming, and embedded systems. He has many scientific papers and participated in several projects in these study fields. He was an IEEE Student Member (2009-2011) and IEEE Member (2011-2014) and has been IEEE Senior Member since 2014.",institutionString:null,institution:{name:"Izmir Kâtip Çelebi University",country:{name:"Turkey"}}},{id:"339677",title:"Dr.",name:"Mrinmoy",middleName:null,surname:"Roy",slug:"mrinmoy-roy",fullName:"Mrinmoy Roy",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/339677/images/16768_n.jpg",biography:"An accomplished Sales & Marketing professional with 12 years of cross-functional experience in well-known organisations such as CIPLA, LUPIN, GLENMARK, ASTRAZENECA across different segment of Sales & Marketing, International Business, Institutional Business, Product Management, Strategic Marketing of HIV, Oncology, Derma, Respiratory, Anti-Diabetic, Nutraceutical & Stomatological Product Portfolio and Generic as well as Chronic Critical Care Portfolio. A First Class MBA in International Business & Strategic Marketing, B.Pharm, D.Pharm, Google Certified Digital Marketing Professional. Qualified PhD Candidate in Operations and Management with special focus on Artificial Intelligence and Machine Learning adoption, analysis and use in Healthcare, Hospital & Pharma Domain. Seasoned with diverse therapy area of Pharmaceutical Sales & Marketing ranging from generating revenue through generating prescriptions, launching new products, and making them big brands with continuous strategy execution at the Physician and Patients level. Moved from Sales to Marketing and Business Development for 3.5 years in South East Asian Market operating from Manila, Philippines. Came back to India and handled and developed Brands such as Gluconorm, Lupisulin, Supracal, Absolut Woman, Hemozink, Fabiflu (For COVID 19), and many more. In my previous assignment I used to develop and execute strategies on Sales & Marketing, Commercialization & Business Development for Institution and Corporate Hospital Business portfolio of Oncology Therapy Area for AstraZeneca Pharma India Ltd. Being a Research Scholar and Student of ‘Operations Research & Management: Artificial Intelligence’ I published several pioneer research papers and book chapters on the same in Internationally reputed journals and Books indexed in Scopus, Springer and Ei Compendex, Google Scholar etc. Currently, I am launching PGDM Pharmaceutical Management Program in IIHMR Bangalore and spearheading the course curriculum and structure of the same. I am interested in Collaboration for Healthcare Innovation, Pharma AI Innovation, Future trend in Marketing and Management with incubation on Healthcare, Healthcare IT startups, AI-ML Modelling and Healthcare Algorithm based training module development. I am also an affiliated member of the Institute of Management Consultant of India, looking forward to Healthcare, Healthcare IT and Innovation, Pharma and Hospital Management Consulting works.",institutionString:null,institution:{name:"Lovely Professional University",country:{name:"India"}}},{id:"1063",title:"Prof.",name:"Constantin",middleName:null,surname:"Volosencu",slug:"constantin-volosencu",fullName:"Constantin Volosencu",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/1063/images/system/1063.png",biography:"Prof. Dr. Constantin Voloşencu graduated as an engineer from\nPolitehnica University of Timișoara, Romania, where he also\nobtained a doctorate degree. He is currently a full professor in\nthe Department of Automation and Applied Informatics at the\nsame university. Dr. Voloşencu is the author of ten books, seven\nbook chapters, and more than 160 papers published in journals\nand conference proceedings. He has also edited twelve books and\nhas twenty-seven patents to his name. He is a manager of research grants, editor in\nchief and member of international journal editorial boards, a former plenary speaker, a member of scientific committees, and chair at international conferences. His\nresearch is in the fields of control systems, control of electric drives, fuzzy control\nsystems, neural network applications, fault detection and diagnosis, sensor network\napplications, monitoring of distributed parameter systems, and power ultrasound\napplications. He has developed automation equipment for machine tools, spooling\nmachines, high-power ultrasound processes, and more.",institutionString:"Polytechnic University of Timişoara",institution:{name:"Polytechnic University of Timişoara",country:{name:"Romania"}}},{id:"221364",title:"Dr.",name:"Eneko",middleName:null,surname:"Osaba",slug:"eneko-osaba",fullName:"Eneko Osaba",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/221364/images/system/221364.jpg",biography:"Dr. Eneko Osaba works at TECNALIA as a senior researcher. He obtained his Ph.D. in Artificial Intelligence in 2015. He has participated in more than twenty-five local and European research projects, and in the publication of more than 130 papers. He has performed several stays at universities in the United Kingdom, Italy, and Malta. Dr. Osaba has served as a program committee member in more than forty international conferences and participated in organizing activities in more than ten international conferences. He is a member of the editorial board of the International Journal of Artificial Intelligence, Data in Brief, and Journal of Advanced Transportation. He is also a guest editor for the Journal of Computational Science, Neurocomputing, Swarm, and Evolutionary Computation and IEEE ITS Magazine.",institutionString:"TECNALIA Research & Innovation",institution:{name:"Tecnalia",country:{name:"Spain"}}},{id:"275829",title:"Dr.",name:"Esther",middleName:null,surname:"Villar-Rodriguez",slug:"esther-villar-rodriguez",fullName:"Esther Villar-Rodriguez",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/275829/images/system/275829.jpg",biography:"Dr. Esther Villar obtained a Ph.D. in Information and Communication Technologies from the University of Alcalá, Spain, in 2015. She obtained a degree in Computer Science from the University of Deusto, Spain, in 2010, and an MSc in Computer Languages and Systems from the National University of Distance Education, Spain, in 2012. Her areas of interest and knowledge include natural language processing (NLP), detection of impersonation in social networks, semantic web, and machine learning. Dr. Esther Villar made several contributions at conferences and publishing in various journals in those fields. Currently, she is working within the OPTIMA (Optimization Modeling & Analytics) business of TECNALIA’s ICT Division as a data scientist in projects related to the prediction and optimization of management and industrial processes (resource planning, energy efficiency, etc).",institutionString:"TECNALIA Research & Innovation",institution:{name:"Tecnalia",country:{name:"Spain"}}},{id:"49813",title:"Dr.",name:"Javier",middleName:null,surname:"Del Ser",slug:"javier-del-ser",fullName:"Javier Del Ser",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/49813/images/system/49813.png",biography:"Prof. Dr. Javier Del Ser received his first PhD in Telecommunication Engineering (Cum Laude) from the University of Navarra, Spain, in 2006, and a second PhD in Computational Intelligence (Summa Cum Laude) from the University of Alcala, Spain, in 2013. He is currently a principal researcher in data analytics and optimisation at TECNALIA (Spain), a visiting fellow at the Basque Center for Applied Mathematics (BCAM) and a part-time lecturer at the University of the Basque Country (UPV/EHU). His research interests gravitate on the use of descriptive, prescriptive and predictive algorithms for data mining and optimization in a diverse range of application fields such as Energy, Transport, Telecommunications, Health and Industry, among others. In these fields he has published more than 240 articles, co-supervised 8 Ph.D. theses, edited 6 books, coauthored 7 patents and participated/led more than 40 research projects. He is a Senior Member of the IEEE, and a recipient of the Biscay Talent prize for his academic career.",institutionString:"Tecnalia Research & Innovation",institution:null},{id:"278948",title:"Dr.",name:"Carlos Pedro",middleName:null,surname:"Gonçalves",slug:"carlos-pedro-goncalves",fullName:"Carlos Pedro Gonçalves",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRcmyQAC/Profile_Picture_1564224512145",biography:'Carlos Pedro Gonçalves (PhD) is an Associate Professor at Lusophone University of Humanities and Technologies and a researcher on Complexity Sciences, Quantum Technologies, Artificial Intelligence, Strategic Studies, Studies in Intelligence and Security, FinTech and Financial Risk Modeling. He is also a progammer with programming experience in:\n\nA) Quantum Computing using Qiskit Python module and IBM Quantum Experience Platform, with software developed on the simulation of Quantum Artificial Neural Networks and Quantum Cybersecurity;\n\nB) Artificial Intelligence and Machine learning programming in Python;\n\nC) Artificial Intelligence, Multiagent Systems Modeling and System Dynamics Modeling in Netlogo, with models developed in the areas of Chaos Theory, Econophysics, Artificial Intelligence, Classical and Quantum Complex Systems Science, with the Econophysics models having been cited worldwide and incorporated in PhD programs by different Universities.\n\nReceived an Arctic Code Vault Contributor status by GitHub, due to having developed open source software preserved in the \\"Arctic Code Vault\\" for future generations (https://archiveprogram.github.com/arctic-vault/), with the Strategy Analyzer A.I. module for decision making support (based on his PhD thesis, used in his Classes on Decision Making and in Strategic Intelligence Consulting Activities) and QNeural Python Quantum Neural Network simulator also preserved in the \\"Arctic Code Vault\\", for access to these software modules see: https://github.com/cpgoncalves. He is also a peer reviewer with outsanding review status from Elsevier journals, including Physica A, Neurocomputing and Engineering Applications of Artificial Intelligence. Science CV available at: https://www.cienciavitae.pt//pt/8E1C-A8B3-78C5 and ORCID: https://orcid.org/0000-0002-0298-3974',institutionString:"University of Lisbon",institution:{name:"Universidade Lusófona",country:{name:"Portugal"}}},{id:"241400",title:"Prof.",name:"Mohammed",middleName:null,surname:"Bsiss",slug:"mohammed-bsiss",fullName:"Mohammed Bsiss",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/241400/images/8062_n.jpg",biography:null,institutionString:null,institution:null},{id:"276128",title:"Dr.",name:"Hira",middleName:null,surname:"Fatima",slug:"hira-fatima",fullName:"Hira Fatima",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/276128/images/14420_n.jpg",biography:"Dr. Hira Fatima\nAssistant Professor\nDepartment of Mathematics\nInstitute of Applied Science\nMangalayatan University, Aligarh\nMobile: no : 8532041179\nhirafatima2014@gmal.com\n\nDr. Hira Fatima has received his Ph.D. degree in pure Mathematics from Aligarh Muslim University, Aligarh India. Currently working as an Assistant Professor in the Department of Mathematics, Institute of Applied Science, Mangalayatan University, Aligarh. She taught so many courses of Mathematics of UG and PG level. Her research Area of Expertise is Functional Analysis & Sequence Spaces. She has been working on Ideal Convergence of double sequence. She has published 17 research papers in National and International Journals including Cogent Mathematics, Filomat, Journal of Intelligent and Fuzzy Systems, Advances in Difference Equations, Journal of Mathematical Analysis, Journal of Mathematical & Computer Science etc. She has also reviewed few research papers for the and international journals. She is a member of Indian Mathematical Society.",institutionString:null,institution:null},{id:"414880",title:"Dr.",name:"Maryam",middleName:null,surname:"Vatankhah",slug:"maryam-vatankhah",fullName:"Maryam Vatankhah",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Borough of Manhattan Community College",country:{name:"United States of America"}}},{id:"414879",title:"Prof.",name:"Mohammad-Reza",middleName:null,surname:"Akbarzadeh-Totonchi",slug:"mohammad-reza-akbarzadeh-totonchi",fullName:"Mohammad-Reza Akbarzadeh-Totonchi",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Ferdowsi University of Mashhad",country:{name:"Iran"}}},{id:"414878",title:"Prof.",name:"Reza",middleName:null,surname:"Fazel-Rezai",slug:"reza-fazel-rezai",fullName:"Reza Fazel-Rezai",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"American Public University System",country:{name:"United States of America"}}},{id:"302698",title:"Dr.",name:"Yao",middleName:null,surname:"Shan",slug:"yao-shan",fullName:"Yao Shan",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Dalian University of Technology",country:{name:"China"}}},{id:"125911",title:"Prof.",name:"Jia-Ching",middleName:null,surname:"Wang",slug:"jia-ching-wang",fullName:"Jia-Ching Wang",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"National Central University",country:{name:"Taiwan"}}},{id:"357085",title:"Mr.",name:"P. Mohan",middleName:null,surname:"Anand",slug:"p.-mohan-anand",fullName:"P. Mohan Anand",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Indian Institute of Technology Kanpur",country:{name:"India"}}},{id:"356696",title:"Ph.D. Student",name:"P.V.",middleName:null,surname:"Sai Charan",slug:"p.v.-sai-charan",fullName:"P.V. Sai Charan",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Indian Institute of Technology Kanpur",country:{name:"India"}}},{id:"357086",title:"Prof.",name:"Sandeep K.",middleName:null,surname:"Shukla",slug:"sandeep-k.-shukla",fullName:"Sandeep K. Shukla",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Indian Institute of Technology Kanpur",country:{name:"India"}}},{id:"356823",title:"MSc.",name:"Seonghee",middleName:null,surname:"Min",slug:"seonghee-min",fullName:"Seonghee Min",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Daegu University",country:{name:"Korea, South"}}},{id:"353307",title:"Prof.",name:"Yoosoo",middleName:null,surname:"Oh",slug:"yoosoo-oh",fullName:"Yoosoo Oh",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:"Yoosoo Oh received his Bachelor's degree in the Department of Electronics and Engineering from Kyungpook National University in 2002. He obtained his Master’s degree in the Department of Information and Communications from Gwangju Institute of Science and Technology (GIST) in 2003. In 2010, he received his Ph.D. degree in the School of Information and Mechatronics from GIST. In the meantime, he was an executed team leader at Culture Technology Institute, GIST, 2010-2012. In 2011, he worked at Lancaster University, the UK as a visiting scholar. In September 2012, he joined Daegu University, where he is currently an associate professor in the School of ICT Conver, Daegu University. Also, he served as the Board of Directors of KSIIS since 2019, and HCI Korea since 2016. From 2017~2019, he worked as a center director of the Mixed Reality Convergence Research Center at Daegu University. From 2015-2017, He worked as a director in the Enterprise Supporting Office of LINC Project Group, Daegu University. His research interests include Activity Fusion & Reasoning, Machine Learning, Context-aware Middleware, Human-Computer Interaction, etc.",institutionString:null,institution:{name:"Daegu Gyeongbuk Institute of Science and Technology",country:{name:"Korea, South"}}},{id:"262719",title:"Dr.",name:"Esma",middleName:null,surname:"Ergüner Özkoç",slug:"esma-erguner-ozkoc",fullName:"Esma Ergüner Özkoç",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Başkent University",country:{name:"Turkey"}}},{id:"346530",title:"Dr.",name:"Ibrahim",middleName:null,surname:"Kaya",slug:"ibrahim-kaya",fullName:"Ibrahim Kaya",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Izmir Kâtip Çelebi University",country:{name:"Turkey"}}},{id:"419199",title:"Dr.",name:"Qun",middleName:null,surname:"Yang",slug:"qun-yang",fullName:"Qun Yang",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"University of Auckland",country:{name:"New Zealand"}}},{id:"351158",title:"Prof.",name:"David W.",middleName:null,surname:"Anderson",slug:"david-w.-anderson",fullName:"David W. Anderson",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"University of Calgary",country:{name:"Canada"}}}]}},subseries:{item:{id:"92",type:"subseries",title:"Health and Wellbeing",keywords:"Ecology, Ecological, Nature, Health, Wellbeing, Health production",scope:"\r\n\tSustainable approaches to health and wellbeing in our COVID 19 recovery needs to focus on ecological approaches that prioritize our relationships with each other, and include engagement with nature, the arts and our heritage. This will ensure that we discover ways to live in our world that allows us and other beings to flourish. We can no longer rely on medicalized approaches to health that wait for people to become ill before attempting to treat them. We need to live in harmony with nature and rediscover the beauty and balance in our everyday lives and surroundings, which contribute to our well-being and that of all other creatures on the planet. This topic will provide insights and knowledge into how to achieve this change in health care that is based on ecologically sustainable practices.
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Fungal infectious illness prevalence and prognosis are determined by the exposure between fungi and host, host immunological state, fungal virulence, and early and accurate diagnosis and treatment. \r\nPatients with both congenital and acquired immunodeficiency are more likely to be infected with opportunistic mycosis. Fungal infectious disease outbreaks are common during the post- disaster rebuilding era, which is characterised by high population density, migration, and poor health and medical conditions.\r\nSystemic or local fungal infection is mainly associated with the fungi directly inhaled or inoculated in the environment during the disaster. The most common fungal infection pathways are human to human (anthropophilic), animal to human (zoophilic), and environment to human (soilophile). Diseases are common as a result of widespread exposure to pathogenic fungus dispersed into the environment. \r\nFungi that are both common and emerging are intertwined. In Southeast Asia, for example, Talaromyces marneffei is an important pathogenic thermally dimorphic fungus that causes systemic mycosis. Widespread fungal infections with complicated and variable clinical manifestations, such as Candida auris infection resistant to several antifungal medicines, Covid-19 associated with Trichoderma, and terbinafine resistant dermatophytosis in India, are among the most serious disorders. \r\nInappropriate local or systemic use of glucocorticoids, as well as their immunosuppressive effects, may lead to changes in fungal infection spectrum and clinical characteristics. Hematogenous candidiasis is a worrisome issue that affects people all over the world, particularly ICU patients. CARD9 deficiency and fungal infection have been major issues in recent years. Invasive aspergillosis is associated with a significant death rate. Special attention should be given to endemic fungal infections, identification of important clinical fungal infections advanced in yeasts, filamentous fungal infections, skin mycobiome and fungal genomes, and immunity to fungal infections.\r\nIn addition, endemic fungal diseases or uncommon fungal infections caused by Mucor irregularis, dermatophytosis, Malassezia, cryptococcosis, chromoblastomycosis, coccidiosis, blastomycosis, histoplasmosis, sporotrichosis, and other fungi, should be monitored. \r\nThis topic includes the research progress on the etiology and pathogenesis of fungal infections, new methods of isolation and identification, rapid detection, drug sensitivity testing, new antifungal drugs, schemes and case series reports. It will provide significant opportunities and support for scientists, clinical doctors, mycologists, antifungal drug researchers, public health practitioners, and epidemiologists from all over the world to share new research, ideas and solutions to promote the development and progress of medical mycology.",coverUrl:"https://cdn.intechopen.com/series_topics/covers/4.jpg",keywords:"Emerging Fungal Pathogens, Invasive Infections, Epidemiology, Cell Membrane, Fungal Virulence, Diagnosis, Treatment"},{id:"5",title:"Parasitic Infectious Diseases",scope:"Parasitic diseases have evolved alongside their human hosts. In many cases, these diseases have adapted so well that they have developed efficient resilience methods in the human host and can live in the host for years. Others, particularly some blood parasites, can cause very acute diseases and are responsible for millions of deaths yearly. Many parasitic diseases are classified as neglected tropical diseases because they have received minimal funding over recent years and, in many cases, are under-reported despite the critical role they play in morbidity and mortality among human and animal hosts. The current topic, Parasitic Infectious Diseases, in the Infectious Diseases Series aims to publish studies on the systematics, epidemiology, molecular biology, genomics, pathogenesis, genetics, and clinical significance of parasitic diseases from blood borne to intestinal parasites as well as zoonotic parasites. We hope to cover all aspects of parasitic diseases to provide current and relevant research data on these very important diseases. In the current atmosphere of the Coronavirus pandemic, communities around the world, particularly those in different underdeveloped areas, are faced with the growing challenges of the high burden of parasitic diseases. At the same time, they are faced with the Covid-19 pandemic leading to what some authors have called potential syndemics that might worsen the outcome of such infections. Therefore, it is important to conduct studies that examine parasitic infections in the context of the coronavirus pandemic for the benefit of all communities to help foster more informed decisions for the betterment of human and animal health.",coverUrl:"https://cdn.intechopen.com/series_topics/covers/5.jpg",keywords:"Blood Borne Parasites, Intestinal Parasites, Protozoa, Helminths, Arthropods, Water Born Parasites, Epidemiology, Molecular Biology, Systematics, Genomics, Proteomics, Ecology"},{id:"6",title:"Viral Infectious Diseases",scope:"The Viral Infectious Diseases Book Series aims to provide a comprehensive overview of recent research trends and discoveries in various viral infectious diseases emerging around the globe. The emergence of any viral disease is hard to anticipate, which often contributes to death. A viral disease can be defined as an infectious disease that has recently appeared within a population or exists in nature with the rapid expansion of incident or geographic range. This series will focus on various crucial factors related to emerging viral infectious diseases, including epidemiology, pathogenesis, host immune response, clinical manifestations, diagnosis, treatment, and clinical recommendations for managing viral infectious diseases, highlighting the recent issues with future directions for effective therapeutic strategies.",coverUrl:"https://cdn.intechopen.com/series_topics/covers/6.jpg",keywords:"Novel Viruses, Virus Transmission, Virus Evolution, Molecular Virology, Control and Prevention, Virus-host Interaction"}],annualVolumeBook:{},thematicCollection:[],selectedSeries:null,selectedSubseries:null},seriesLanding:{item:null},libraryRecommendation:{success:null,errors:{},institutions:[]},route:{name:"profile.detail",path:"/profiles/207156",hash:"",query:{},params:{id:"207156"},fullPath:"/profiles/207156",meta:{},from:{name:null,path:"/",hash:"",query:{},params:{},fullPath:"/",meta:{}}}},function(){var e;(e=document.currentScript||document.scripts[document.scripts.length-1]).parentNode.removeChild(e)}()