In commercial micropropagation laboratories, the light source is one of the most important factors controlling plant morphogenesis and metabolism of plant cells and tissue and organ cultures. Lamp manufacturers have begun to rate lamps specifically for plant needs. The traditional light source used for in vitro propagation is fluorescent lamps (FLs). However, power consumption in FL use is expensive and produces a wide range of wavelengths (350–750 nm) unnecessary for plant development. Light‐emitting diodes (LEDs) have recently emerged as an alternative for commercial micropropagation. The flexibility of matching LED wavelengths to plant photoreceptors may provide more optimal production, influencing plant morphology and chlorophyll content. Although previous reports have confirmed physiological effects of LED light quality on morphogenesis and growth of several plantlets in vitro, these study results showed that LED light is more suitable for plant morphogenesis and growth than FLs. However, the responses vary according to plant species. This chapter describes the applications and benefits of LED lamps on chlorophyll in plant micropropagation. Two study cases are exposed, Anthurium (Anthurium andreanum) and moth orchids (Phalaenopsisis sp.), both species with economic importance as ornamental plants, where LEDs have a positive effect on in vitro development and chlorophyll content.
Part of the book: Chlorophyll
Micropropagation of sugarcane is important to obtain pathogen-free plants, genetically homogeneous and invigorate. The micropropagation procedure is divided into stages for the sake of better understanding. Micropropagation for large-scale sugarcane production using a temporary immersion system (TIS) is described. In addition, the aim of this chapter is to report, from the laboratory to the field, the best way to establish and use basic seed (primary seed), semicommercial seed (foundation or secondary seed) and commercial seed production. In conclusion, commercial sugarcane micropropagation enables the massive multiplication of plants to obtain certified vitroplants and increase the sugarcane and sugar productivity per unit area.
Part of the book: Sugarcane