Examples of soil amplicon sequencing done so far covering different habitat types.
\\n\\n
\\n"}]',published:!0,mainMedia:{caption:"Milestone",originalUrl:"/media/original/124"}},components:[{type:"htmlEditorComponent",content:'
Barely three months into the new year and we are happy to announce a monumental milestone reached - 150 million downloads.
\n\nThis achievement solidifies IntechOpen’s place as a pioneer in Open Access publishing and the home to some of the most relevant scientific research available through Open Access.
\n\nWe are so proud to have worked with so many bright minds throughout the years who have helped us spread knowledge through the power of Open Access and we look forward to continuing to support some of the greatest thinkers of our day.
\n\nThank you for making IntechOpen your place of learning, sharing, and discovery, and here’s to 150 million more!
\n\n\n\n\n'}],latestNews:[{slug:"webinar-introduction-to-open-science-wednesday-18-may-1-pm-cest-20220518",title:"Webinar: Introduction to Open Science | Wednesday 18 May, 1 PM CEST"},{slug:"step-in-the-right-direction-intechopen-launches-a-portfolio-of-open-science-journals-20220414",title:"Step in the Right Direction: IntechOpen Launches a Portfolio of Open Science Journals"},{slug:"let-s-meet-at-london-book-fair-5-7-april-2022-olympia-london-20220321",title:"Let’s meet at London Book Fair, 5-7 April 2022, Olympia London"},{slug:"50-books-published-as-part-of-intechopen-and-knowledge-unlatched-ku-collaboration-20220316",title:"50 Books published as part of IntechOpen and Knowledge Unlatched (KU) Collaboration"},{slug:"intechopen-joins-the-united-nations-sustainable-development-goals-publishers-compact-20221702",title:"IntechOpen joins the United Nations Sustainable Development Goals Publishers Compact"},{slug:"intechopen-signs-exclusive-representation-agreement-with-lsr-libros-servicios-y-representaciones-s-a-de-c-v-20211123",title:"IntechOpen Signs Exclusive Representation Agreement with LSR Libros Servicios y Representaciones S.A. de C.V"},{slug:"intechopen-expands-partnership-with-research4life-20211110",title:"IntechOpen Expands Partnership with Research4Life"},{slug:"introducing-intechopen-book-series-a-new-publishing-format-for-oa-books-20210915",title:"Introducing IntechOpen Book Series - A New Publishing Format for OA Books"}]},book:{item:{type:"book",id:"3749",leadTitle:null,fullTitle:"Evolutionary Computation",title:"Evolutionary Computation",subtitle:null,reviewType:"peer-reviewed",abstract:"This book presents several recent advances on Evolutionary Computation, specially evolution-based\r\noptimization methods and hybrid algorithms for several applications, from optimization and learning to pattern recognition and bioinformatics. \r\n\r\nThis book also presents new algorithms based on several analogies and metafores, where one of them is based on philosophy, specifically on the philosophy of praxis and dialectics. In this book it is also presented interesting applications on bioinformatics, specially the use of particle swarms to discover gene expression patterns in DNA microarrays. Therefore, this book features representative work on the field of evolutionary computation and applied sciences. \r\n\r\nThe intended audience is graduate, undergraduate, researchers, and anyone who wishes to become familiar with the latest research work on this field.",isbn:null,printIsbn:"978-953-307-008-7",pdfIsbn:"978-953-51-5913-1",doi:"10.5772/234",price:159,priceEur:175,priceUsd:205,slug:"evolutionary-computation",numberOfPages:584,isOpenForSubmission:!1,isInWos:null,isInBkci:!1,hash:"413cf10893f15955d121a4934493da16",bookSignature:"Wellington Pinheiro dos Santos",publishedDate:"October 1st 2009",coverURL:"https://cdn.intechopen.com/books/images_new/3749.jpg",numberOfDownloads:74524,numberOfWosCitations:85,numberOfCrossrefCitations:72,numberOfCrossrefCitationsByBook:5,numberOfDimensionsCitations:130,numberOfDimensionsCitationsByBook:5,hasAltmetrics:0,numberOfTotalCitations:287,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"May 16th 2013",dateEndSecondStepPublish:"June 6th 2013",dateEndThirdStepPublish:"September 10th 2013",dateEndFourthStepPublish:"December 9th 2013",dateEndFifthStepPublish:"January 8th 2014",currentStepOfPublishingProcess:5,indexedIn:"1,2,3,4,5,6,7",editedByType:"Edited by",kuFlag:!1,featuredMarkup:null,editors:[{id:"125844",title:"Prof.",name:"Wellington",middleName:"Pinheiro Dos",surname:"Santos",slug:"wellington-santos",fullName:"Wellington Santos",profilePictureURL:"https://mts.intechopen.com/storage/users/125844/images/4878_n.jpg",biography:"Wellington Pinheiro dos Santos holds a degree in Electrical and Electronics Engineering (2001) and MS in Electrical Engineering (2003) from the Federal University of Pernambuco, and Ph.D. in Electrical Engineering from the Federal University of Campina Grande (2009). He is currently a Professor (exclusive devotion) of the Department of Biomedical Engineering at the Center of Technology and Geosciences - Engineering School of Pernambuco, Federal University of Pernambuco, acting in Undergraduate Biomedical Engineering and Graduate Program in Biomedical Engineering, which was one of the founders (2011). He founded the Center for Social Technologies and Bioengineering, at the Federal University of Pernambuco, NETBio-UFPE (2012). He is a member of the Graduate Program in Computer Engineering from the Polytechnic School of Pernambuco, University of Pernambuco, since 2009. He also has experience in the area of Computer Science, with an emphasis on Graphic Processing (Graphics), acting on the following themes: digital image processing, pattern recognition, computer vision, evolutionary computation, numerical methods of optimization, computational intelligence, computer graphics, virtual reality, game design and applications of Computing and Engineering in Medicine and Biology. 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\r\n\tThis book provides information about up-to-date wind energy technologies in the context of smart grids. It starts with an introduction of wind energy characteristics and the availability prediction of wind energy in wind farms. As the wind power proportion increases in power systems, significant uncertainties and technical challenges arise to cause the concerns of the network stability and power dispatch. These bring about some latest technologies to cover the major aspects of energy integration and economic dispatch in order to meet energy demands and targets. Market tools such as pricing, gaming, bidding, and auction design as well as the “prosumer” concept will also be discussed, followed by extensive economic analysis and power dispatch optimization. Finally, cybersecurity will be studied to promote the uptake of energy Internet of Things (IoT) and also to understand electric grid security and data security.
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\r\n\tAll book chapters are produced by forward-thinking specialists in the area of renewable energy and smart grids, with detailed analysis and/or case studies. This book is intended to serve as a reference for graduate students, academics, professionals, and system operators.
Soil is a robust and brilliantly vast ecosystem (2000–8.3 million bacterial species per gram). Therefore, it serves as a vast reservoir for microorganisms inhabiting in a niche that is different within the specific soil ecosystem, which can be pathogenic or beneficial [1, 2, 3, 4]. Each proportion of soil whether in grasslands, forests, or deserts (i.e., sand, silt, clay, and organic matter) offers habitats for nematodes and a large number of microbes that vary from bacteria and are also useful in nutrient cycling [5, 6, 7, 8]. Moreover, the distinct microhabitat dwelled by microorganisms with the capability to adjust and established their colony to the specific niche [9]. The crucial factors which influence the microbial load in the soil ecosystem include soil pH, organic compound, and temperature [10, 11, 12]. The chemical or physical activity does not merely determine the development of soil but the constant unfolding of different microbial species, which include or may improve the attributes of soils, regarding the development of function and structure [13, 14]. Soil supplies protection to different soil harboring animals, reptiles, and insects, along with a tremendous number of microbes inside the soil aggregates [15].
In this direction, the field of metagenomics continues to be a ground-breaking technology, which has made it possible to explore microbial diversity with its full potential [16]. Besides the soil ecosystem, microbes could quickly react to anthropogenic pressures, making it feasible to be an indication of soil quality as well as wellness [17, 18]. Lately, efforts have been attempted to determine genes from environmental samples via culture-independent techniques [19, 20]. However, they had been amplified or perhaps recognized due to their similarity to the earlier identified genes, that invalidates for exploiting novel elements of metal resistance [21, 22]. As the development of culture-independent metagenomic methods, it has been employed to evaluate the soil microbial community as well as enhances our awareness of the soil ecosystem [17, 20]. Furthermore, the soil microbial communities are primarily made up of some dominant species and numerous other rare taxa [23].
The ones with a low abundance might be from some novel microbial lineages and might play a vital function in biogeochemical interactions of the soil–plant system [24]. Therefore, the information obtained from the full metagenomic sequencing is crucial to expose the genomic data of low abundance populations as well as to disclose their activity in the soil [6]. It has been effectively released into investigating numerous varied microbial niches in the human gut, grassland soil, and aquatic ecosystem [25, 26, 27]. Furthermore, attempts have been established to evaluate the abundance of soil microbes as well as the genes involved in heavy metals’ opposition from agricultural soils [28, 29]. Additionally, soil metagenomics beyond estimating the soil microorganisms can also help in getting a concept about the soil and its habitat based on the different soil types [30]. This chapter provides the importance of soil metagenomics and standard methods of analysis, along with challenges and prospects of soil metagenomics.
Soil is an interconnected system because of its microorganisms despite getting incredible and unique capability to adjust to life changes; soil microbes are hypersensitive to land management and also weather changes [1, 11, 14]. Based on this information, our ancestors learned the ability to grow plants and created different cultivation methods like inoculating mycorrhizal fungi with food and floral crops to decrease the impact of soil-borne diseases [31, 32, 33, 34]. With a most varied ecosystem with a composition of known and unknown microbial species, the soil provides an ecological niche [34, 35]. The biochemistry of soil reflects many anonymous functions that are a lot essential for sustenance of life [35, 36, 37]. Nevertheless, the latest technologies utilizing heavy machinery and management methods intensified agriculture and have resulted in the degeneration of the cultivable farmlands through damage of fertility, soil structure as well as the soil microbial life [38, 39, 40]. In a nutshell, lots of arable areas have switched to uncultivable or saline soil [38]. Agricultural land is simultaneously getting forfeited to nonagricultural uses [39]. The generation of soil, which primarily contains carbon twice as much as the atmosphere, is a complex phenomenon and requires lots of years for the formation of 1 cm topsoil [41]. Metagenomics data can be used to investigate the gene sequencing helpful in microbial symbiosis, as this is the most ancient symbiosis of nature of around 400 million years [42]. With the increased population pressure, the concern around worldwide sustainability also increases. Therefore, improving and sustaining the qualities of soil is an utmost concern for many years. Thus, soil health gets among the most crucial aspects of agriculture [8, 24].
Metagenomics offers an entirely new method of looking at the microbial community that has transformed contemporary microbiology and also has the potential to revolutionize comprehension approaches of the various ecosystems [43, 44]. In metagenomics, the strength of genomic examination is put on to whole populations of microorganisms [45]. Metagenomics approaches are throwing light on the myriad abilities of microbial communities that operate the planet’s energy and nutrient cycles and form the evolution of life [46, 47]. Metagenomics is anticipated to produce awareness of microbial interactions; therefore, it is used to enhance human well-being, energy production, and food security [48]. Metagenomics combines the strength of genomics, systems biology and bioinformatics and power of genomics within the research of communities generates an unparalleled ability [43, 45]. Metagenomics, still a very new science, but has produced insightful information about the microbial community due to its radically unique means of realizing the microbial world [49, 50]. The diverse test of DNA may subsequently be analyzed directly, or even cloned into a type maintainable in lab bacteria, developing a library which has the genomes of all of the microbes present in that environment [51, 52, 53]. Nevertheless, the launch of the culture-independent approaches eliminates the obstacles and barriers in understanding the environmental samples [30].
Metagenomics initially targeted the shotgun sequencing; these days it’s just as helpful for the scientific studies regarding marker genes
It is well recognized that the microorganisms have an abundant quantity and diversity than other organisms on the planet [4]. Nevertheless, the division of the microbial diversity at global scales is still partially understood. The microbial diversity and composition structure are significantly affected by environmental elements [62]. As a result, indexing, cataloging and proof of the microorganisms are prerequisites for the exploration [62]. Microbial diversity in any habitat is more related to the substantial amount of species existed at a specific time [63, 64]. As the earth microbial community plays essential roles in soil health management, agro-ecosystem, accessibility of growing nutrition as well as turnover tasks of organic material in soil, they are hugely influenced by both anthropogenic and natural activities [65, 66]. For instance, many microbes that are helpful to the ecosystem services are currently threatened because of inferior agricultural practices, local weather transforming patterns, ground as well as land degradation, etc. [67, 68]. In recent years, the use of artificial fertilizers, herbicides, fungicides, and other pesticides has resulted in the deterioration of the soil microflora and diversity [7, 31, 69]. Therefore, the microorganisms with the changing atmosphere will offer a broader picture of the way the microbes are shifting the functional characteristics of soils and their flourishing in the endangered ecosystems [69, 70].
Nowadays, most of the environmental focus in agriculture is on achieving agricultural sustainability. Many metagenomic initiatives have been completed in the area of agriculture but do not hold some promise to assist the marginal farmers [71, 72, 73]. Therefore, productive scientific studies are required, which might be used the growers’ income and help agriculture [74]. The latest advances in the soil metagenomics emerge as an extraordinary area of research because of the assignment of understanding the associated microorganisms in development and plant growth [75, 76]. Likewise, restoration of the microbial population was determined to improve grain yield as well as soil health [77]. Metagenomics can predict the soil microbes’ structure and the impact on microbial groups of connected niches [9, 35].
Sustainable agricultural methods consist of different microhabitats with excellent environmental fluctuations and genetic biodiversity [78]. Reports from agrarian soils confirmed that there are high microbial stock and plant development promotion pursuits [79]. Many studies are showing the latest metagenomic improvements in agriculture [76, 77, 78]. Soil microbes play a crucial part for triggering the plant development, stress reactions, as well as defense in vegetation [80, 81]. Understanding the connection between the soil microbiota and plants using soil metagenomics is hugely advantageous in developing the crop systems [1, 82]. Metagenomics research of the soils supplemented with organic manures from several farm animals will be a lot valuable in formulating the fertilization tactics [12, 65]. For renewable agricultural production, helpful microbes of agricultural value can function as an essential alternative [39, 73]. Metagenomics compensation can address basic restorative questions associated with agriculturally significant microorganisms [83].
Direct DNA extraction and characterization through PCR and metagenomic survey have developed the study of soil ecosystem [81]. Applying metagenomics of plant-microbial association can be used to study interaction with beneficial microbes among pathogenic strains, infect recently, profitable endosymbionts inside these beneficial microbes (AMF) like nitrifying bacteria, (phosphate solubilizing bacteria) PSB and plant growth-promoting abilities are found [84]. The specificity of plant-microbial symbiosis development can be easily understood at the molecular level both for agronomic and horticulture crops, using forward and reverse genetic approaches [85]. As reported earlier, microbial inoculation has the potential of increasing plant production and sustainability in agricultural fields, so the metagenomics study can reveal the distinct microbial strains interacting with which chemical compound in the mycorrhizospheric soil and to acknowledge community structure, horizontal gene transfer analysis and phylogeny of microbes interacting with other environmental factors [86, 87]. Also, the exact niche information of microbial communities infecting soil adhering to the roots, surface between roots and soil, the surface of roots, or colonizing with the roots, can be drawn [87]. As we know this interaction is bi-directional, plant gets the essential nutrients from the soil, and in return, these rhizospheric and/or root microbiotas get the photosynthesis-derived organic compounds, and this process is known as rhizodeposition [88]. Thus, this crucial symbiosis that underlying plant-microbe community associations can be easily implicit by metagenomics for agricultural purposes because the NGS which can determine the relative abundance of microbe whether it is culturable in laboratory conditions or not [89]. In addition, the results of hundreds of samples simultaneously can be obtained on the same day as the samples are loaded [89].
In the amplicon sequencing study, first, soil DNA is extracted, and next, 16S/18S rDNA sequences are amplified using a specific set of particular primers targeting variable areas of 16S/18S rDNA, accompanied by filtration of fragments using magnetic beads [54, 90, 91, 92]. Consequently, adapters are ligated, and the library of fragments (clones) is amplified along with the samples are sequenced utilizing NGS platform (Figure 1). The dataset obtained after sequencing is used for the identification of microbial diversity [54, 55]. Using NGS and the related software, it is doable to solve extremely complicated microbiota compositions with greater precision and to relate the microbial ecosystem of the soil [16, 55, 92], although, it must be considered for accurate data and analysis interpretation while choosing amplicon sequencing working with marker genes [92].
Metagenomic analysis of environmental microbial sampling based on nucleic acids.
From the start of metagenomics, the study of novel metabolite/biomolecule (DNA polymerases, cellulases, lipases/esterases, chitinases and antibiotics) from the microbial assembly was its first application, and this has advanced with the development of NGS techniques for calculating comparison between community metagenome, meta-proteomics, and meta-transcriptomics [93, 94]. Techniques for recovering novel metabolite that comprise cloning of the microbial DNA from the environment then constructing a small/large-insert libraries, which can be done either by function-based or sequence-based screening of metagenomic libraries are shown in Table 1. The resulting metagenomic libraries subsequently transformed in several hosts like Escherichia coli (mostly),
Origin | Sequencing platform/amplicon analysis technique | Total sequencing size | Country | Results | References |
---|---|---|---|---|---|
Potato field | Pyrosequencing | 1674 OTUs | USA | Identification of potato soil- borne pathogens | [95] |
Soil of 3 islands in the Yellow Sea | Pyrosequencing | 10,166 reads | South Korea | Wood decomposing, plant-parasitic, endophytic, ectomycorrhizal and saprotrophic fungi | [96] |
6 sites of forest and grassland soils | Pyrosequencing | 598,962 sequences | Germany | Identification of 17 bacterial phyla and 4 proteobacterial classes | [97] |
Pea field | Pyrosequencing | 55,460 sequences | Denmark | Fungal species, diversity, community composition of phylum Ascomycota, and Basidiomycota | [98] |
Hitchiti | Deep Ion Torrent sequencing | >3,000,000 sequences | USA | 12 fungal strains identification | [99] |
Sossego copper mine | Pyrosequencing | 10,978 OTUs | Brazil | 36 bacterial phyla and five proteobacteria classes | [100] |
Riverine Wetland soil | Illumina system | 1872 OTUs | USA | 56 different bacterial phyla | [101] |
Solid biomedical dumpsites | Illumina system | 1,706,442 sequences | Tanzania | 31 bacterial phyla belonging to aromatic hydrocarbons degraders, chitin degraders, chlorophenol degraders and atrazine metabolizers | [102] |
Grave-soil human cadavers | Illumina system | 1,729,482 reads | USA | 45 decomposing microbes identification | [103] |
Zea mays fields | Illumina MiSeq | 2,453,023 reads | UK, France, Italy | Comparative account of soil microorganisms of three different sites | [104] |
Pepper field | Illumina system | 4147 OTUs | Spain | Studying soil-borne pathogens | [105] |
Solid waste dumping site, Chite river site, Turial river site, Tuikual river site | Illumina system | 111,3884 sequences | India | Identified 27 proteobacteria and bacteroidetes | [106] |
Tomato | Illumina amplicon sequencing analysis and phytohormone measurements | 337,961 high-quality reads and 647 fungal OTUs | Denmark | Identification of 27 endophytic fungi and root hormone quantification | [107] |
Examples of soil amplicon sequencing done so far covering different habitat types.
Metagenomics was initiated with the aim of DNA cloning and screening, and now it has made significant advances in microbiology, evolution, and ecology [91, 92]. These first projects not merely proved the concept of the metagenomics but additionally unraveled enormous gene diversity within the microbial world. The various steps in soil metagenomics are enlisted below and shown in Figure 2.
The layout of metagenomics showing collection of samples from agricultural field and analysis.
Sampling is the first and crucial step. The extracted DNA must be of high quality for metagenomic library construction and sequencing. Further, fractionation or selective lysis is ideal for those communities which are linked to the plethora. Fractionation should be examined for adequate target enrichment with little contamination.
Metagenomic sequencing significantly depends upon the sequencing platforms used. Nowadays, NGS techniques viz. Illumina/Solexa systems, 454/Roche sequencing, and Oxford nanopore sequencing technologies are being continuously used for metagenomic projects. Contigs are essential in getting the whole length sequence. So, assembly of short reads becomes key in metagenomics which may be accomplished by co-assembly and de novo assembly methods. On the flip side, the de novo assembly needs sophisticated computational tools and assemblers (e.g. MetaVelvet, and Meta-IDBA).
Binning shows the process of sorting of DNA in several groups of individual genomes.
In the very first step, binning explores the conserved nucleotide composition of genomes. Then, the DNA fragments are searched against a reference to bin the sequence. The binning algorithms use structure and similarity, like MetaCluster and PhymmBL. If the goal of the analysis of the reconstructed genome and large contigs, in this particular strategy, little length of contigs should be 30,000 bp or even longer. In future prediction of the assembled sequences, labelling is done while functional annotation includes mapping with an existing database. The sequences which cannot be mapped provide an endless amount of novelty in metagenomic samples. Several reference databases can be utilized to supply functional annotation viz. TIGRFAM, KEGG, eggNOG, PFAM, etc.
Statistical assessment of the metagenomic data is vital for the exploration of the significance of the results. However, it must have appropriate experimental designs with proper replications. Metagenomic data sharing involves a great computational framework as well as a storage facility. Several of the centralized services have typical formats for recording and documenting experimental details.
Robust extraction, as well as characterization of the DNA of soil microbiota through amplicon sequencing, has revolutionized the studies of ecology and environmental sciences. Essentially, the metagenomic evaluation of nucleic acids gives immediate access to the genomes of the uncultivated majority of underexploited microbial life. Accelerated by developments in sequencing technologies, microbiologists have found more novel species, genera, as well as genes from microorganisms. The unprecedented range of soil types continued exploration of a variety of agricultural and environmental features. The capacity to check out earth microbial communities with increasing capability has presumably the highest promise for answering numerous mysteries of the microbial world. Molecular methods, which include metagenomics, have revolutionized the analysis of microbial ecology. We cannot link virtually all microorganisms to their metabolic roles within an earth community. Increased sequencing capability provided by high throughput sequencing technologies has assisted characterize as well as quantify soil diversity. However, these methodologies are usually leveraged to process more samples at a reasonably shallow depth as compared to survey throughout the genomes from a single sample adequately. Figure 3 describes the various application of metagenomics.
A brief account of applications of metagenomics in different fields.
Along with higher diversity, methodological biases produce a considerable challenge for soil microbial characterization. These biases include soil sampling, DNA extraction, adsorption of nucleic acids to soil particles, contributions of extracellular DNA, sample planning, sequencing protocols, sequence analysis, and purposeful annotation. Since current sequencing technologies produce millions of reads, difficulties linked to interpreting these results can contribute to the problems encountered by microbial ecologists in determining the involvement of various microorganisms in the number of processes of soil. Without having a suitable benchmark methodology or dataset for verifying the fidelity of amplicon or perhaps metagenomic analyses, assessing whether the presence, as well as the activity of organisms, are adequately evaluated, is impossible. Furthermore, methodological limitations which might stop the detection of some active and abundant bacteria in soil could lead to the same essential amount of misinterpretation. No individual protocol would be seen as adequate in isolation of DNA. Likewise, the taxonomic and likely useful deciphering of the soil microbiota would critically gain from a blend of strategies.
Exact replicates are challenging to obtain due to soil microorganism compositional changes. An additional challenge would be that the total number of species that are in a single sample of soil is unfamiliar, with hugely varying estimates. One crucial very first step toward dealing with several of the problems experienced by soil microbiologists is actually to start developing a substantial catalog of all microbial community members and features for no less than one reference soil. Such a relatively comprehensive reference dataset would shed light on the as-yet-unknown design of a ground microbial species frequency distribution and might serve as an ultimate guide for assessing town composition switches across soil landscapes (i.e., beta diversity). Put simply, the scope of bias with any private strategy (i.e., a one-time DNA extraction method) might be explicitly driven by comparing extraction strategies coupled with detailed characterization of the selected reference soil. For instance, the isolation, as well as characterization of cells via single-cell genomics, can assist target phylogenetically analysis. Coupled with extensive DNA based characterization of the collected guide soil microbial diversity, this specific research initiative should ideally assess several levels of gene expression, at the amount of RNA (metatranscriptomics), proteins (metaproteomics), and also metabolites (metametabolomics). By identifying the way a reference soil is structured, both temporally and spatially, the info from this coordinated effort might help supply missing links between typical soil analyses as well as the underlying composition of soil microbial communities.
An in-depth exploration of single guide soil should involve experiments much beyond the typical metagenomic analyses applied to soil samples. Instead, this effort is going to require considerable benchmarking of the sampling technique itself, which is connected to identifying a suitable resource website. Such an endeavor would call for a coordinated inter-disciplinary consortium of knowledge spanning chemistry, soil physics, biochemistry, microbiology, and bioinformatics. The outcomes of the effort can develop an objective foundation for creating standardized protocols for ongoing and future soil microbiological investigations.
Man is essentially a myth-maker—using myths, folklore, fable and legend to contextualise the society in which he lives. Since goats have been a companion of man in the midst of his society since the dawn of civilisation, it is only logical that goats feature in oral tradition. Oral tradition that, over millennia, has been captured in the written myths, legends, epics, fable and fairy tales that we know today.
Many cultures have used goats in stories that portray various social constructs of their times. But here, it may be important to first digress and provide some analysis of the different types of folklore. Folktales, specifically, can be compared across cultures through recurring ideas or ‘motifs’ (dominant themes), which are defined by the political, economic and religious discourse of the time. Motifs may be situations, actions, events, characters or objects. Very often, the motifs are abstractions or models, the details being secondary and differing according to the variant: thus the typical situation (motif) of the hero outwitting the stupid giant or giants is the same whether the hero is a god, a prince, a young huntsman, a retired soldier or the Three Billy Goats Gruff. The purse of gold may be a jug of oil that never empties, the bird that lays the golden eggs, a plant that provides eternal life or the little goat that bleats causing a table laden with food to appear to the melancholic (but always attractive), ill-treated, stepsister [1]. It can be said that once we have identified the motif behind a folktale, we can then determine whether the goat has been used as a cursory object to creatively illustrate the story or whether the goat is the actual subject of the story.
Very often, in folktales, goats are used for creative illustration (sometimes the goat could just as well have been a pig, cow or chicken). But, other times, goats convey a specific meaning to the story and play a role in the motif that cannot be as accurately illustrated by another animal [2].
Folklore includes popular traditions, legendary ballads, local proverbial sayings, superstitions, old customs, folktales, legends, games, sports and nursery rhymes. Edward Clodd believed that the chief function of folklore was to explore the savage beliefs and practices underlying established religion. However, folktales also provide insight into human psychology through their underlying meaning [3].
Myth is generally distinguished from legends and fairy tales in that the former addresses cosmic themes such as the creation of the world, the beginning of life and the origins of civilisation. They may have arisen as embellished accounts of historical events, as a means to justify religious ritual or as metaphor or representation of natural phenomena [4]. Mythic stories are enacted on a supernatural scale: heroes of myth are gods and superhumans. Heroes of fairy tales and legends are ordinary men and women [2] although magic and fantastic animals, creatures or events may be involved.
Fables are a somewhat different genre. It is generally thought that fables were the creation of the slave Aesop who lived on the island of Samos in the sixth century BC. He won the favour of Croesus, king of Lydia, (and his freedom) by telling stories [5]. Aesop’s fables have an industrious, efficient style, always ending with a moral. They are mainly for adult enjoyment and generally depict animals’ instinctive behaviour in humanistic terms: the ant is industrious, the fox is crafty, and the tortoise is slow. Fable ‘morals’ or ‘motifs’ are centred on the notion that ‘life is a constant struggle’ and of ‘survival of the fittest’ [2].
Whereas fables are indifferent and realistic, the animals of European fairy tale are more compassionate and magical. Fairy tales are generally more optimistic and joyful than fables (although this is disputed by some and proven by some stories with unhappy endings [6]), are characterised by a lack of realism, are not believed by those who tell them and generally aim to teach [2]. Fables tend to depict animals as merciless and irresponsible, while fairy tales make them intelligent and benevolent. Fables tend to depict animals in terms of behaviourism, while the fairy tale reminds one of the analytical psychologies of Freud or Jung [2]. A distinction has been made that fairy tale and myth bring animals closer to men, while fables and satire, while seemingly doing the same thing, do the reverse; they are conflict-ridden and put animals in their place—as submissive to humans [2].
However, as we may all have experienced, the interaction with a live animal is a very fundamental and personal encounter, which largely negates the limitations often imposed by cultural perception. Thus, the goat, being an animal of unique intelligence, virility and behaviour, so close to man because of its usefulness and hardiness, has characteristics that have been described in the same way among different cultures. The goat is depicted as a symbol of fertility, abundance, aggression and good luck in many of these civilisations.
These cross-cultural similarities and differences are of interest but, also, the changes in how these animals are depicted over time warrant scrutiny. It is surely modifications in human and social conditions that create or cause these fluctuations in how goats are depicted because the character of goats is a genetic rather than an environmental manifestation.
This chapter investigates the role played by goats in the myths and folklore of various Ancient Near Eastern (ANE) cultures. To this effect, images in artefacts, and metaphors or direct reference to goats in texts (to be considered the primary sources in this study) from the area are studied. Secondary sources provided the context within which the sources occur. Where images and references to goats occur, their meaning and relationship to belief systems or their underlying ‘motifs’ within the ANE cultures are identified and discussed. Ultimately, this chapter aims to determine whether ANE cultures ascribed certain characteristics to goats that may still be applicable today.
Although the array of domesticated animals is vast, this analysis investigates primarily goats (and similar species such as the ibex where relevant or where ambiguity occurs) in the myth, folklore and fairy tale (if these can be defined) of the Near Eastern cultures of Mesopotamia, Sumer, Akkad, Anatolia and Ancient Iran (Elam). The timespan that is covered by this chapter is approximately 3000 years. It is acknowledged that this is too vast an expanse to make specific inferences about the impact of the goat on the political, social or religious contexts within which goat symbolism may occur, nor can one surmise at the influence that this political, social or religious context makes on the way the goat is considered in society.
It is believed that wild animals were herded into enclosures for killing to improve on the success of hunting—images depicting this practice have been found [7]. After a time, animals that exhibited certain characteristics that would make them predisposed to domestication became accustomed to this close contact. This close association between man and animal led to the domestication [8] of some species (but not others). These ‘phases of domestication’ can be categorised into ‘wild’, ‘managed’ and ‘domesticated’ [9] states.
Jared Diamond [10] has suggested several characteristics that would predispose certain species to domestication, and these include:
High fertility and reproductive rate and a fast growth rate
Animals that can be herded and handled without becoming aggressive
Animals that are adaptable to a range of environments and feedstuffs that are not directly in competition with man’s diet
Animals with a docile nature that are not prone to flightiness or skittishness
Animals that naturally move in herds, and submit to a pack leader
In all these characteristics, the goat excels.
According to Gilbert [11], wild sheep (Ovis) and goats (Capra) were the earliest livestock domesticated during the Palaeolithic to Neolithic transition (9000–7000 B.C.E.) in northern and eastern Mesopotamia. Pigs (Sus) and cattle (Bos) were predominantly domesticated in southern Mesopotamia. Later introductions included horses (Equus 2500 B.C.E.), donkeys (Equus 4000–3000 B.C.E.) and chickens (
Recent research [12] has placed the process of the domestication of goats in the region of south-western Asia, from the eastern Mediterranean to Turkey and from the southern Levant though south-eastern Turkey and northern Syria to the high Zagros mountain pastures and arid lowland plains of Iraq and Iran. The process of domestication spanned approximately 500 years—culminating around 8000 B.C.E. Zeder and Hesse [12] identified a distinct profile of young male goat slaughter and prolonged female survivorship (or delayed slaughter of females) in herds in the upland areas of northern Iraq and north-western Iran through the analysis of goat remains. Animal production involves the practice of keeping females for breeding and slaughtering males at a younger age for meat. This is in contrast to the remains of hunted populations, which clearly show a focus on fully adult males with females and young taken only occasionally.
The ancestors of the modern goat were potentially two species,
Most of the domesticated animals we know today originated in the Ancient Near East. These include cattle, goats, pigs, sheep, cats, dogs, ducks, chickens, geese, horses, donkeys and mules [8]. Their use for milk, meat, fat, leather, wool, hair, draught, transportation, manure [8], cheese [9], the storage of wealth [11], eggs [9], recreational (and possibly educational) zoos [9], drugs [9] and for religious use (sacrifice, extispicy and for maintenance of the religious complex) is documented.
Sheep and goats were kept in large flocks that belonged to the state, the temple or private owners and each was branded with its owner’s mark (or that of the god for which it was intended). Clearly articulated contracts between owners and shepherds have been found. Shepherds often kept all the dairy products and some of the wool as compensation for their work. Their compensation could also include a share of the growth in the flock. Figure 1 shows terracotta tablets inscribed with the records of goatherds and cowherds [14].
Terracotta tablets inscribed with the records of goatherds (left) and cowherds (right), unearthed at Lagash, Mesopotamia, and dated to 2250–2175 B.C.E. (housed in the collection at the Louvre, Paris: [
Wool (from sheep) and hair (from goats) were initially plucked (pulled or combed), but shearing was practised from the middle of the second millennium. Milk was obtained from cows, sheep and goats (camels and water buffalo were also used for this purpose). Yoghurt, cheese, butter and ghee were produced. Butter was made by churning the milk in a narrow-necked jar [7] and rolled with the foot or rocked [9]. Since fresh milk spoiled rapidly, it was often only used in medicines, which would be made fresh and used immediately. Figure 2 shows a cylinder seal from Mari with a typical agricultural scene. Many such seals have been found, which may indicate the economic activity in which the owner of the seal may have distinguished him (or her)self.
Cylinder seal from Mari (3000–2000 B.C.E.). Top register showing milking scene, bottom register with goats, hero figures and eagle with lion-head.
Although horses were important in battle, goats were often taken as booty (Figure 3) and inflated goat skin bags were used in warfare as flotation devices (Figure 4).
Stone panel from the central palace of Tiglath-Pileser III. Neo-Assyrian, 730–727 B.C.E. Nimrud (ancient Kalhu), northern Iraq (housed in the British Museum).
Goat skins used as flotation devices. Stone panel from the central palace of Tiglath-Pileser III. Neo-Assyrian, 730–727 B.C.E. Nimrud (ancient Kalhu), northern Iraq (housed in the British Museum).
Primary sources available to the modern scholar of the ANE cultures include objects, architecture and written texts that have survived environmental extremes. This investigation seeks to determine whether, if goats appear in these material artefacts, there is some indication that they were considered more than merely useful as a livestock species with utilitarian value but also as an object in myth, legend or fairy tale.
Several texts excavated from ancient Mesopotamia refer to animals in general [15]. These can be categorised into the following types:
Wordlists
Omen collections (omina) (including guidelines involving extispicy and apotropaic rituals)
Myths
Short, functional fables, with no expressed moral
Personified animals in longer narrative fables, debates or contests
Mentioned in propagandistic literature as being subjugated, hunted, collected, etc. by the king.
The consumption of animals (as well as recipes)
In humorous and ironic anecdotes.
For several of these categories, examples were found in a collection of Sumerian inscriptions [16, 17]. If evident, this categorisation is shown and possible explanations for the texts are provided in parentheses.
Page 99. 3.111—‘Although it has never gone there, the goat knows the wasteland’ (Category: ironic anecdote. Possible explanation: a goat has instinct to survive in the desert, or a goat is intelligent—this perhaps relates to the idea of someone having a ‘gut feeling’ about something)
Page 101. 3.123—‘May you hold a kid in your right arm and may you hold a bribe in your left arm’ (Category: ironic anecdote. Possible explanation: the kid can be brought as an offering—to request a favour from the god—but if the offering does not work, you also can back it up with a bribe to the priest who can implore the god on your behalf. This is interesting, since it suggests that people were quite aware that priests could ‘bend the rules’ if requested/motivated to do so).
Page 106. 3.153—‘The goat spoke in the manner of a (wise) old woman, but acted in the manner of an unclean woman’ (Category: ironic anecdote. Possible explanation: goats are considered intelligent, but they are bawdy and eat everything including unclean things, which make them unclean. Here, the sexual nature of the goat is identified).
Page 128. 5.55—‘A lion had caught a helpless she-goat’ (and she said), ‘Let me go and I will give you my fellow ewe in return.’ ‘If I let you go, tell me first your name!’ The she-goat answered the lion, ‘You don’t know my name? “I am cleverer than you” is my name.’ After the lion had come to the sheepfold he roared, ‘I released you!’ She answered from the other side, ‘You released me, you are clever,… the sheep are not here!’ (Category: narrative contest. Possible explanation: again showing the intelligence of the goat. Alster [16, 17] suggests that this is the Sumerian fable that resembles closest to a true ‘Aesopian’ one. The Sumerian fables provide a definite answer to the much discussed question, whether or not the fable as a genre was invented by the Greeks. Although the genre is now proven to be considerably older than the Greeks, it does not necessarily mean, however, that it was first invented in Mesopotamia).
Page 167. 8.Sec.B4.—‘Wearing a long beard like a goat’ (Category: humorous anecdote. Possible explanation: merely a physical description of a ‘goatee’ beard)
Page 167. 8.Sec.B5.—‘A goat is the gift of a large kid’ (Category: ironic anecdote. Possible explanation: goats can reproduce at a very young age—as young as 4 months).
Page 167. 8.Sec.B6.—“A goat speaks as follows to another goat: I also toss my head.” (Category: ironic anecdote. Possible explanation: animals of the same species are likely to behave in the same way. Or, people of a certain character can identify with another person of the same character, that is, it takes one to know one).
Page 169. 8.Sec.B8—‘When the wolves were pursuing a goat, it turned around, and its feet stumbled over its own feet’ (Category: humorous anecdote. Possible explanation: a goat is ‘tripsy-footed’?)
Page 171. 8.Sec.B28.—‘A fox spoke to a goat, “Let me put my shoes in your house!” The goat answered, “When the dog comes, let me hang them on a nail!” The fox answered, “If the dog stays like that in your house, bring me my shoes. Let me not stay till midnight!”’ (Category: narrative contest. Possible explanation: this dialogue again suggests the intelligence of the goat in being able to outwit another creature).
Goats are evident in the mythology of ANE as shown in various imagery. Two types of artefacts are available to the art historian [18]: objects in the round and two-dimensional objects. Objects carved in the round include figurines, theriomorphic vessels and weights. Figurines and animal-shaped vessels were often rendered in baked clay and rhyta (singular rhyton) in metal. Many of these objects were used in the temple complexes as votive objects (being brought as tribute to a god), as apotropaic figures (buried underneath the floors of buildings to ward off evil), were used during religious ceremonies or were used to weigh the produce brought to the temple by the citizens who formed part of the distributive temple economies. Two-dimensional objects included low-relief stelae, perforated panels in stone or ceramic, tablets and hollow carved stamps or cylinder seals. These objects had decorative use in temple architecture (stelae and panels). Tablets were used as administrative tools (to record numbers of livestock or produce), to train scribes, to capture true literature and royal inscriptions or to write letters. Stamps and cylinder seals were personal signatures used to identify objects or show ownership [19].
A general search for images of goats on ANE artefacts was undertaken. This was done through a search of archaeological research papers and books, which either provided references to relevant artefacts or showed photographs or tracings of artefacts. Where these items were considered important but the images were unclear, higher quality images were sought. Museum collections that have been made available for public viewing via the Internet were also examined.
This was followed by a classification and comparison of the images of the various artefacts.
To provide structure to the comparison, images that depict a similar theme or ‘motif’ were grouped. To this effect, a method was sought to provide some meaning to a classification. Whereas some scholars have argued that the depiction of animals in Mesopotamian art illustrates the start of religion, others have argued that animals provide merely a symbolic representation of the world as the artist saw it. However, it must be agreed that there must be multiple levels of meaning. To this end, the system provided by Root [20] to classify the use of animals in art is used here. An approximate seriation within each group was also undertaken (although it is conceded that the chronology provided by scholars commenting on these pieces is assumed correct, which may not necessarily be the case). The groupings made are discussed below.
The goat lends itself to abstraction and decoration with its curved horns and upturned tail—this is especially the case in the use of the goat (or ibex) in the art of Ancient Anatolia. The depiction of goats in Mesopotamian art is often stereotypical, allowing one to assume that sketchbooks were used and artists trained in standard doctrines. In the artefacts of this group, the artists have attempted to depict goats in their most recognisable form (Figures 5 and 6). These include full profile; walking, or standing with their horned heads shown from the side; or lying down. Mostly, single animals are shown. Where goats are not shown entirely, they are suggested by parts of their body, such as horns in the goat-head rhyton (Figure 7).
Elamite mountain goat from Susa, 3100–2900 B.C.E. (housed in the Louvre, Paris).
A baked clay pot, sporting a goat, from Umma which is dated to the Amorite dynasty of the nineteenth–eighteenth century B.C.E. (housed at the Louvre, Paris: [
(a) Burnished pottery rhyton in the form of a goat’s head from Iran (Elam) that is dated to 1000 B.C.E. (b and c) Earthenware goat rhyton dated to between 1000 and 2000 B.C.E. (All housed at the Sankokan University, Japan).
Although the objects and images shown here have been placed in the category of ‘abstraction and decorative composition’ because they are particularly striking, some inferences regarding their symbolism can be made. Firstly, these objects may have served as votive objects, as apotropaic figurines or as libation vessels (as part of a ritual in which characteristics of the animal depicted are expected to be imbibed by the drinker). Furthermore, it is interesting to note that in both objects of Figures 5 and 6, the goat is shown with its right leg foremost. As Green [21] suggests in a paper dealing with apotropaic figurines (that lead with the left foot), this may be the beginnings of the custom of entering a holy place with the right foot first.
Generally, ancient Mesopotamian gods were human in form. But both demonic and virtuous creatures were often depicted as animal-human hybrids. Specific animals were associated with certain gods and often became their symbols [9]. Thus, the dog was associated with Gula, the god of healing; the lion-snake-eagle with Marduk and the goat-fish with Ea (or Enki) [9]. Hatziminaglou and Boyazoglu [22], however, state that goats were sacred to Marduk and is often pictured accompanied by a goat (unfortunately, they do not reference this statement and are perhaps confusing images of Ea with Marduk).
Goats were an important element of divining the future [9, 23]: firstly, through unsolicited omens or observations (as from the omen list ‘If a City is Situated on an Elevation’ where both domesticated and wild animals were used) and secondly, through solicited omens through extispicy (reading of entrails) or merely a ritual involving an animal that did not necessarily involve its slaughter.
Where a god had an associated animal, as is the case, for example, with Ea or Enki, worshippers could bring tribute to a god either by the live goat itself or in the form of their animal statue (these votive objects could be clay, gold, bronze and/or sliver), or in the form of a figure bringing the goat tribute. Images and figurines depicting this practice (shown in Figure 8) are referred to by modern scholars as ‘goat bearers’ (British Museum). It is interesting to note the abundance of the ‘goat bearer’ imagery over several cultures (Sumerian, Anatolian, Assyrian and Elamite) and over a long expanse of time. Further examples can be found in the work of Marchesi and Marchetti [24], which shows more than five examples from several Early Dynastic Mesopotamian sites.
(a) Plaque from Nippur, temple of In’anak (early dynastic I (3000–2330 B.C.E.)) [
Comparison of these images reveals that all the goat bearers have goatees or beards and all but one (from Zincirli carrying the goat on his neck) bear the goats in his arms. This may be important since, as Breniquet [18] mentions, very often the animal used to represent the god in visual material or in association with him shares the facial features or appearance (physiognomy) and behaviour of the god. The placement of the animal with the god renders the reading unequivocal and is part of the essence of the god.
According to Heinsohn [25], tribute was not so much to the god but to the priests themselves who managed the temple complex, as thanks for the role played by the priest in the act of sacrifice (it could be dangerous), as well as for absorbing the guilt for the killing itself (an act created to calm an emotionally traumatised community—initially following a cataclysmic event). It seems that ritual sacrifice was abandoned when further cataclysmic events no longer occurred and thus sacrifice (used to placate an anxious community) was no longer necessary. At this point, votive objects may have replaced tributes of live animals.
Although modern scholars refer to these images as ‘goat bearers’ (British Museum; [16]), it is not clear from, nor mentioned in, the literature whether these statues represent worshippers, priests or the god Ea (Enki) himself. Where the goat bearers form part of a scene where other tribute is being brought, it seems clear that the goat bearer is indeed a worshipper or tribute-payer; however, where the images are solitary, the identity of the bearer is ambiguous.
Whole herds of animals were kept for their gods by their temples and branded with their marks [9, 23]. One can assume that when live animals were replaced by votive objects as tributes, then, these same herds would not have been used as sacrificial animals, but as a means to support the attendants of the temple complex and to redistribute to the community.
Animals were also used as the recipients of evil transferred from a human host, what we know today as the ‘scapegoat’ ritual [23]. This ‘transferral’ of the evil (whether bad luck or an illness) could be achieved through the patient handling the animal, by the performance of a ritual and then letting the animal go (into a wasteland or a river), by the performance of a ritual and then slaughtering the animal and burying it (or discarding its remains in a river or wasteland, or by splitting the animal in half and passing between the two halves) or by the patient spitting on it, or eating it, or being covered with it (its blood), or by merely having the animal in proximity to the affected person. The scapegoat ritual was often used by the military and sometimes by cities to remove the scourge of a plague [27].
Animal figurines could be used for scapegoat rituals or as apotropaic figurines that were buried under the floors of domestic dwellings, barracks or temples to ward off evil [21]. Elaborate rituals describing an array of figurines to use (including the goat-fish), and the numbers of figurines that are required, have been documented [21].
The name ‘Enki’ literally means Lord, en, of the earth or the netherworld, ki, the patron deity of the apkallus (fish-men), who lived in the underground sweetwaters, the Abzu (Sumerian) or Apsu (Akkadian) [28]. In this imagery, the god Enki/Ea was associated with the ‘Sacred tree’ (often erroneously called the ‘Tree of life’).
The Anzu/Apsu was considered, a not altogether unpleasant intermediary place, between the earth and the netherworld (almost literally the place of spring water from which life springs). This idea may be suggested by the imagery of the goat in the tree (as shown in Figure 9). This was possibly a libation device, and used as temple furniture.
The lapis-lazulli goat buck in the tree from the Royal Cemetery at Ur, belonging to the Uruk period c 2600–2500 B.C.E. was probably temple furniture—possibly a stand (housed in the British Museum).
What is important to note here is that although the goat in the tree represents a certain idea known to the community (Enki and the Sacred tree), and goat imagery is used in furniture or libation devices, the animals themselves were not worshipped [18].
Two examples of the use of goats in this manner were found.
In Anatolian art and folklore, a ‘mistress of the beasts’ occurs, often associated with the hunt. This mistress (and sometimes master) is associated with a feline (usually a lion) and a wild goat, ibex or gazelle [29]. This goddess may have been Cybele (or Dali in Armenia) and may also be related to the goddess associated with the hunt of the Caucasus (Georgians who are believed to be descendants of the Hittites) who is always associated with goats, ibex or turs (
Goddess seated on a goat over two lions from Kultepe, Anatolia, from the Middle Bronze Age, 2000–1595 B.C.E. [
Queen of animals feeds her wild goats, thirteenth B.C.E., from tomb III, Minet el-Beida, harbour of Ugarit, Syria. Lid of pyxis (round box with lid) (housed in the Louvre).
The myths that incorporate Ea (or Enki) are of particular significance here since the primary sources reveal him to be associated with the goat. The spouse of Ea was Damkina and his cult centre was Eridu. Marduk was Ea’s son. During the Kassite period, Marduk was elevated to the top of the Babylonian pantheon and his cult centre was Babylon [19].
Ea plays a role in several of the Mesopotamian myths. In the Epic of Gilgamesh, where aside from being mentioned as one of the gods that have made Gilgamesh wise, he is the god that breaks rank and warns the human Ut-napishtim (whom Gilgamesh seeks to reveal the secret of eternal life since Ut-napishtim survived the flood and has been made immortal) of the impending flood sent by the gods to destroy man. This role of Enki is again shown in the myth of Atrahasis, the Flood story. Here too, Enki warns man about the flood and gives him instruction to build a boat.
Enki plays a larger role in the Epic of Creation. Here, it is explained, in the beginning, there were only two gods, Apsu (who represents the primordial waters under the earth) and Tiamat (the personification of the sea). They beget four generations of gods, who become noisy and unbearable. Apsu decides to put an end to their troublesome ways, but the plot is discovered by Ea ‘who knows everything’. He puts Apsu and his evil vizier, Mummu, to sleep and then slays them. He then assumes the belt, crown and mantle of radiance, takes over the dwelling place of Apsu as his own, and there, with his spouse Damkina, creates Marduk. Marduk then proceeds to win all sorts of fantastic battles with the encouragement of his father, Ea, and is finally made the king of the gods. One of Marduk’s actions is to create man (to do the work of the gods so that the gods can be at leisure).
In shorter myths, again Ea plays a role. Mostly as the ‘one who knows everything’. These include the myth of Adapa (a priest of Ea in his cult temple at Eridu); the Epic of Anzu; the Descent of Ishtar to the Underworld and the myth of Nergal and Ereshkigal.
The relief and apotropaic figures below (Figure 12) depict the god Enki (Sumerian) or Ea (Akkadian). Enki was the god of productivity [31] and freshwater streams, springs and lakes, as well as the abzu (or apsu – the subterranean freshwater ocean on which the earth supposedly floated; [8, 31]) and, in this guise, was symbolized as the (or a) fish [9].
(a) Enki represented as a fish-man in stone relief and (b) as an apotropaic figure (source unknown).
Enki was also associated with wisdom, science, craftsmanship and magic and this part of his nature was symbolised by a goat [9]. Several stone reliefs and cylinder seal impressions were found showing goat bearers, a goat in the scene, or a goat shown below the god with water or fishes also represented.
This ‘dual’ nature of the god ultimately resulted in the use of the symbol of the goat-fish (literally ‘Carp-goat’) for the god Enki or Ea [32] (Akkadian suḫurmašû; Neo-Sumerian Selekuid). Figures of this deity are named in certain apotropaic (averting evil) rituals [21]. Although no Assyrian monumental art of this deity has been found, the requirements for their manufacture (dimensions, gold leaf required etc.) are alluded to in Nimrud texts for a temple of Nabu at Kalhu [32]. Artefacts representing this deity are shown in Figures 13–15.
(a–c) Mesopotamian boundary stones of the Kassite era, 1600–1150 B.C.E. [
(a) A goat-fish from Assur (housed in the Lowie Museum of Anthropology, University of California, Berkeley: [
A cylinder seal, from the late Babylonian era, possibly depicts a worshipper before divine images, including the
The boundary stones each show celestial objects (stars and crescent moons), monuments or temples and deities. On all three boundary stones, the goat-fish (curved or twisted horns and hooves) can be clearly distinguished from the dragon (which is generally depicted with straight horns, talons or claws and a snake-like body).
Similarities between the renditions of goat-fish include one front leg bent back and the other forward (slightly bent and lifted) (although both left leg and right leg forward can be seen), cross-hatchings used to indicate the scales of a fish, twisted splayed horns (except the foundation figurine, which may have had horns that are now broken off). All the boundary stones’ goat-fish are without beards (goatees).
From the preceding source material, several inferences relating to the perception of ANE societies regarding goats can be made. The images shown by the material artefacts, in which goats are portrayed, possess a decorative, symbolic, religious or mythological motive. It is clearly discernible when goats are merely portrayed as a livestock species. These include the goatherd tablets, the agricultural scene in the cylinder seal from Mari, and the battle scene where goats and sheep probably represent booty. It is clear from the (quantitatively) numerous depictions of goats in domestic scenes that goats played an important role in the household and temple economies of the time. In these scenes, it is clear that the goat represents itself and no underlying ‘motif’ or meaning is intended.
Also, generally, goats are rendered very realistically (long, straight or curved horns and upright tail) and in their characteristic attitudes since they were a familiar sight in everyday life. This can be expected, since, in general, variations are most often found in animals that the artists were not as familiar with (such as elephants or giraffes) [33].
The animal-animal hybrid, the goat-fish, representing Ea or Enki, is most probably the basis of the constellation Capricorn. According to Roy [34], all evidence points to the Sumero-Akkadian astronomer priests as the makers of the constellations as we know them. Mesopotamia is found on the required latitude to have seen all the constellations (some are not visible from the southern hemisphere), the position of the stars at that time (3000–2000 B.C.E.) would provide a perfect fit for their observations, and the constellations themselves match almost every deity in the Mesopotamian pantheon, including the goat-fish of Ea, now known as Capricorn (so-named by the Greeks [35]).
In the myth of the creation of the world, Marduk, aside from creating man, also defined the calendar, setting up the Zodiac and the sequence of celestial events that would signify the changing seasons [34]. Star lists in cuneiform have been found, detailing these constellations and their positions relative to each other. The positions of the stars were used for navigation, divining the future and as a means to map boundaries (constellations often appear on boundary stones). Gurshtein [35] agrees that the goat-fish constellation has Sumerian origins. It is interesting to note that the goat-fish icon is called a suḫurmašû [32], whereas Capricorn is called SUHUR.MAS in tablets from Uruk [36].
The use of goat imagery in the artefacts described show the goat in association with the god Enki or Ea, and is used in libation vessels, temple furniture, plaques and votive objects used in the worship of this god. But, it should be noted that it is not the goat that is worshipped.
In literature too, although the goat is associated with the god Enki or Ea, it is the god in his human form that acts in the myths—not the god in animal form. The goat is used to represent the god in visual material or in association with him. This finding is corroborated by Breniquet [18] who states that in iconography, the animal is closely related to the god and acts as his substitute. Of more interest is her statement that the facial features or appearance (physiognomy) and behaviour are also those of the god. The animal is not merely an accessory, enabling the illiterate to interpret the portrayals; the placement of the animal with the god renders the reading unequivocal and is part of the essence of the god.
The Sumerian texts [16, 17] reveal that goats were considered intelligent, wise and cunning. The genetic characteristics of goats such as their ability to reproduce at a young age, their physical appearance (goatees) and their bawdiness are also alluded to in everyday proverbs. Goats are thus used as metaphors for intelligence, mischievousness, virility and fertility.
In each of the Mesopotamian myths described above, Ea or Enki plays the role of the wise god, offering solutions to a myriad of problems. He is described as the god ‘who knows everything’ and ‘the Lord of intelligence’. In the myth of Adapa, it may be suggested that the role of Ea ‘who knows everything’ is to show man that it is better not to have eternal life. Alternatively, here Ea could be assuming the role of the ‘trickster’ often ascribed to goats in later myth and alluded to again in the Aesopian-type dialogues with the lion and the fox in the Sumerian inscriptions shown above.
In the Descent of Ishtar to the Underworld, the role of Ea in creating a ‘playboy’ may allude to the sexual nature of goats. And again in the myth of Nergal and Ereshkigal, the story has an overtly sexual nature.
It is suggested by Root [20] that the use of rhyton as libation vessels originated in the notion that imbibing liquid from a vessel resembling one of the more vital animals would magically convey some of the animal’s own vitality to the drinker. It is perhaps these qualities of intelligence and fertility that worshippers intended to obtain when drinking from rhyton shaped as goats or from libation devices decorated with goat motifs.
The depiction of goats in the art and literature of the Ancient Near Eastern cultures provides us with a means to understand man’s attitudes towards goats during this period in time. From the resources used, it is clear that goats fulfilled an immensely important utilitarian function. The heritage of Enki (Ea) as the constellation of Capricorn is significant. The use of goats in ritual and symbolism has provided us with many motifs related to goats that are still in use today: these include the motifs of fertility, intelligence and craftiness. From all accounts, Enki (Ea) was a benevolent god, always available to solve problems, and often assisting mankind to avert extinction. The imagery associated with Enki/Ea, as in the sacred tree, the sweet waters, the apotropaic suḫurmašû and ultimately the goat, communicates Enki as a symbol of life and an averter of evil.
These are powerfully hopeful and positive images.
The author wishes to thank Professor Paul Kruger of the Department of Ancient Studies at the University of Stellenbosch, South Africa, for his guidance before and during this study, which formed part of the Postgraduate Diploma in Ancient Cultures at Stellenbosch University.
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Applications",isOpenForSubmission:!1,hash:"a3479e76c6ac538aac76409c9efb7e41",slug:"neuroimaging-neurobiology-multimodal-and-network-applications",bookSignature:"Yongxia Zhou",coverURL:"https://cdn.intechopen.com/books/images_new/9347.jpg",editedByType:"Edited by",editors:[{id:"259308",title:"Dr.",name:"Yongxia",middleName:null,surname:"Zhou",slug:"yongxia-zhou",fullName:"Yongxia Zhou"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"8938",title:"Inhibitory Control Training",subtitle:"A Multidisciplinary Approach",isOpenForSubmission:!1,hash:"bd82354f3bba4af5421337cd42052f86",slug:"inhibitory-control-training-a-multidisciplinary-approach",bookSignature:"Sara Palermo and Massimo Bartoli",coverURL:"https://cdn.intechopen.com/books/images_new/8938.jpg",editedByType:"Edited by",editors:[{id:"233998",title:"Ph.D.",name:"Sara",middleName:null,surname:"Palermo",slug:"sara-palermo",fullName:"Sara Palermo"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}}],booksByTopicTotal:66,seriesByTopicCollection:[],seriesByTopicTotal:0,mostCitedChapters:[{id:"58070",doi:"10.5772/intechopen.72427",title:"MRI Medical Image Denoising by Fundamental Filters",slug:"mri-medical-image-denoising-by-fundamental-filters",totalDownloads:2592,totalCrossrefCites:19,totalDimensionsCites:32,abstract:"Nowadays Medical imaging technique Magnetic Resonance Imaging (MRI) plays an important role in medical setting to form high standard images contained in the human brain. MRI is commonly used once treating brain, prostate cancers, ankle and foot. The Magnetic Resonance Imaging (MRI) images are usually liable to suffer from noises such as Gaussian noise, salt and pepper noise and speckle noise. So getting of brain image with accuracy is very extremely task. An accurate brain image is very necessary for further diagnosis process. During this chapter, a median filter algorithm will be modified. Gaussian noise and Salt and pepper noise will be added to MRI image. A proposed Median filter (MF), Adaptive Median filter (AMF) and Adaptive Wiener filter (AWF) will be implemented. The filters will be used to remove the additive noises present in the MRI images. The noise density will be added gradually to MRI image to compare performance of the filters evaluation. The performance of these filters will be compared exploitation the applied mathematics parameter Peak Signal-to-Noise Ratio (PSNR).",book:{id:"6144",slug:"high-resolution-neuroimaging-basic-physical-principles-and-clinical-applications",title:"High-Resolution Neuroimaging",fullTitle:"High-Resolution Neuroimaging - Basic Physical Principles and Clinical Applications"},signatures:"Hanafy M. Ali",authors:[{id:"213318",title:"Dr.",name:"Hanafy",middleName:"M.",surname:"Ali",slug:"hanafy-ali",fullName:"Hanafy Ali"}]},{id:"46296",doi:"10.5772/57398",title:"Physiological Role of Amyloid Beta in Neural Cells: The Cellular Trophic Activity",slug:"physiological-role-of-amyloid-beta-in-neural-cells-the-cellular-trophic-activity",totalDownloads:5907,totalCrossrefCites:19,totalDimensionsCites:32,abstract:null,book:{id:"3846",slug:"neurochemistry",title:"Neurochemistry",fullTitle:"Neurochemistry"},signatures:"M. del C. Cárdenas-Aguayo, M. del C. Silva-Lucero, M. Cortes-Ortiz,\nB. Jiménez-Ramos, L. Gómez-Virgilio, G. Ramírez-Rodríguez, E. Vera-\nArroyo, R. Fiorentino-Pérez, U. García, J. Luna-Muñoz and M.A.\nMeraz-Ríos",authors:[{id:"42225",title:"Dr.",name:"Jose",middleName:null,surname:"Luna-Muñoz",slug:"jose-luna-munoz",fullName:"Jose Luna-Muñoz"},{id:"114746",title:"Dr.",name:"Marco",middleName:null,surname:"Meraz-Ríos",slug:"marco-meraz-rios",fullName:"Marco Meraz-Ríos"},{id:"169616",title:"Dr.",name:"Maria del Carmen",middleName:null,surname:"Cardenas-Aguayo",slug:"maria-del-carmen-cardenas-aguayo",fullName:"Maria del Carmen Cardenas-Aguayo"},{id:"169857",title:"Dr.",name:"Maria del Carmen",middleName:null,surname:"Silva-Lucero",slug:"maria-del-carmen-silva-lucero",fullName:"Maria del Carmen Silva-Lucero"},{id:"169858",title:"Dr.",name:"Maribel",middleName:null,surname:"Cortes-Ortiz",slug:"maribel-cortes-ortiz",fullName:"Maribel Cortes-Ortiz"},{id:"169859",title:"Dr.",name:"Berenice",middleName:null,surname:"Jimenez-Ramos",slug:"berenice-jimenez-ramos",fullName:"Berenice Jimenez-Ramos"},{id:"169860",title:"Dr.",name:"Laura",middleName:null,surname:"Gomez-Virgilio",slug:"laura-gomez-virgilio",fullName:"Laura Gomez-Virgilio"},{id:"169861",title:"Dr.",name:"Gerardo",middleName:null,surname:"Ramirez-Rodriguez",slug:"gerardo-ramirez-rodriguez",fullName:"Gerardo Ramirez-Rodriguez"},{id:"169862",title:"Dr.",name:"Eduardo",middleName:null,surname:"Vera-Arroyo",slug:"eduardo-vera-arroyo",fullName:"Eduardo Vera-Arroyo"},{id:"169863",title:"Dr.",name:"Rosana Sofia",middleName:null,surname:"Fiorentino-Perez",slug:"rosana-sofia-fiorentino-perez",fullName:"Rosana Sofia Fiorentino-Perez"},{id:"169864",title:"Dr.",name:"Ubaldo",middleName:null,surname:"Garcia",slug:"ubaldo-garcia",fullName:"Ubaldo Garcia"}]},{id:"41589",doi:"10.5772/50323",title:"The Role of the Amygdala in Anxiety Disorders",slug:"the-role-of-the-amygdala-in-anxiety-disorders",totalDownloads:9707,totalCrossrefCites:4,totalDimensionsCites:28,abstract:null,book:{id:"2599",slug:"the-amygdala-a-discrete-multitasking-manager",title:"The Amygdala",fullTitle:"The Amygdala - A Discrete Multitasking Manager"},signatures:"Gina L. Forster, Andrew M. Novick, Jamie L. Scholl and Michael J. Watt",authors:[{id:"145620",title:"Dr.",name:"Gina",middleName:null,surname:"Forster",slug:"gina-forster",fullName:"Gina Forster"},{id:"146553",title:"BSc.",name:"Andrew",middleName:null,surname:"Novick",slug:"andrew-novick",fullName:"Andrew Novick"},{id:"146554",title:"MSc.",name:"Jamie",middleName:null,surname:"Scholl",slug:"jamie-scholl",fullName:"Jamie Scholl"},{id:"146555",title:"Dr.",name:"Michael",middleName:null,surname:"Watt",slug:"michael-watt",fullName:"Michael Watt"}]},{id:"26258",doi:"10.5772/28300",title:"Excitotoxicity and Oxidative Stress in Acute Ischemic Stroke",slug:"excitotoxicity-and-oxidative-stress-in-acute-ischemic-stroke",totalDownloads:7183,totalCrossrefCites:6,totalDimensionsCites:25,abstract:null,book:{id:"931",slug:"acute-ischemic-stroke",title:"Acute Ischemic Stroke",fullTitle:"Acute Ischemic Stroke"},signatures:"Ramón Rama Bretón and Julio César García Rodríguez",authors:[{id:"73430",title:"Prof.",name:"Ramon",middleName:null,surname:"Rama",slug:"ramon-rama",fullName:"Ramon Rama"},{id:"124643",title:"Prof.",name:"Julio Cesar",middleName:null,surname:"García",slug:"julio-cesar-garcia",fullName:"Julio Cesar García"}]},{id:"62072",doi:"10.5772/intechopen.78695",title:"Brain-Computer Interface and Motor Imagery Training: The Role of Visual Feedback and Embodiment",slug:"brain-computer-interface-and-motor-imagery-training-the-role-of-visual-feedback-and-embodiment",totalDownloads:1455,totalCrossrefCites:13,totalDimensionsCites:24,abstract:"Controlling a brain-computer interface (BCI) is a difficult task that requires extensive training. Particularly in the case of motor imagery BCIs, users may need several training sessions before they learn how to generate desired brain activity and reach an acceptable performance. A typical training protocol for such BCIs includes execution of a motor imagery task by the user, followed by presentation of an extending bar or a moving object on a computer screen. In this chapter, we discuss the importance of a visual feedback that resembles human actions, the effect of human factors such as confidence and motivation, and the role of embodiment in the learning process of a motor imagery task. Our results from a series of experiments in which users BCI-operated a humanlike android robot confirm that realistic visual feedback can induce a sense of embodiment, which promotes a significant learning of the motor imagery task in a short amount of time. We review the impact of humanlike visual feedback in optimized modulation of brain activity by the BCI users.",book:{id:"6610",slug:"evolving-bci-therapy-engaging-brain-state-dynamics",title:"Evolving BCI Therapy",fullTitle:"Evolving BCI Therapy - Engaging Brain State Dynamics"},signatures:"Maryam Alimardani, Shuichi Nishio and Hiroshi Ishiguro",authors:[{id:"11981",title:"Prof.",name:"Hiroshi",middleName:null,surname:"Ishiguro",slug:"hiroshi-ishiguro",fullName:"Hiroshi Ishiguro"},{id:"231131",title:"Dr.",name:"Maryam",middleName:null,surname:"Alimardani",slug:"maryam-alimardani",fullName:"Maryam Alimardani"},{id:"231134",title:"Dr.",name:"Shuichi",middleName:null,surname:"Nishio",slug:"shuichi-nishio",fullName:"Shuichi Nishio"}]}],mostDownloadedChaptersLast30Days:[{id:"29764",title:"Underlying Causes of Paresthesia",slug:"underlying-causes-of-paresthesia",totalDownloads:192987,totalCrossrefCites:3,totalDimensionsCites:7,abstract:null,book:{id:"1069",slug:"paresthesia",title:"Paresthesia",fullTitle:"Paresthesia"},signatures:"Mahdi Sharif-Alhoseini, Vafa Rahimi-Movaghar and Alexander R. Vaccaro",authors:[{id:"91165",title:"Prof.",name:"Vafa",middleName:null,surname:"Rahimi-Movaghar",slug:"vafa-rahimi-movaghar",fullName:"Vafa Rahimi-Movaghar"}]},{id:"63258",title:"Anatomy and Function of the Hypothalamus",slug:"anatomy-and-function-of-the-hypothalamus",totalDownloads:4596,totalCrossrefCites:6,totalDimensionsCites:12,abstract:"The hypothalamus is a small but important area of the brain formed by various nucleus and nervous fibers. Through its neuronal connections, it is involved in many complex functions of the organism such as vegetative system control, homeostasis of the organism, thermoregulation, and also in adjusting the emotional behavior. The hypothalamus is involved in different daily activities like eating or drinking, in the control of the body’s temperature and energy maintenance, and in the process of memorizing. It also modulates the endocrine system through its connections with the pituitary gland. Precise anatomical description along with a correct characterization of the component structures is essential for understanding its functions.",book:{id:"6331",slug:"hypothalamus-in-health-and-diseases",title:"Hypothalamus in Health and Diseases",fullTitle:"Hypothalamus in Health and Diseases"},signatures:"Miana Gabriela Pop, Carmen Crivii and Iulian Opincariu",authors:null},{id:"57103",title:"GABA and Glutamate: Their Transmitter Role in the CNS and Pancreatic Islets",slug:"gaba-and-glutamate-their-transmitter-role-in-the-cns-and-pancreatic-islets",totalDownloads:3523,totalCrossrefCites:3,totalDimensionsCites:9,abstract:"Glutamate and gamma-aminobutyric acid (GABA) are the major neurotransmitters in the mammalian brain. Inhibitory GABA and excitatory glutamate work together to control many processes, including the brain’s overall level of excitation. The contributions of GABA and glutamate in extra-neuronal signaling are by far less widely recognized. In this chapter, we first discuss the role of both neurotransmitters during development, emphasizing the importance of the shift from excitatory to inhibitory GABAergic neurotransmission. The second part summarizes the biosynthesis and role of GABA and glutamate in neurotransmission in the mature brain, and major neurological disorders associated with glutamate and GABA receptors and GABA release mechanisms. The final part focuses on extra-neuronal glutamatergic and GABAergic signaling in pancreatic islets of Langerhans, and possible associations with type 1 diabetes mellitus.",book:{id:"6237",slug:"gaba-and-glutamate-new-developments-in-neurotransmission-research",title:"GABA And Glutamate",fullTitle:"GABA And Glutamate - New Developments In Neurotransmission Research"},signatures:"Christiane S. Hampe, Hiroshi Mitoma and Mario Manto",authors:[{id:"210220",title:"Prof.",name:"Christiane",middleName:null,surname:"Hampe",slug:"christiane-hampe",fullName:"Christiane Hampe"},{id:"210485",title:"Prof.",name:"Mario",middleName:null,surname:"Manto",slug:"mario-manto",fullName:"Mario Manto"},{id:"210486",title:"Prof.",name:"Hiroshi",middleName:null,surname:"Mitoma",slug:"hiroshi-mitoma",fullName:"Hiroshi Mitoma"}]},{id:"35802",title:"Cross-Cultural/Linguistic Differences in the Prevalence of Developmental Dyslexia and the Hypothesis of Granularity and Transparency",slug:"cross-cultural-linguistic-differences-in-the-prevalence-of-developmental-dyslexia-and-the-hypothesis",totalDownloads:3609,totalCrossrefCites:2,totalDimensionsCites:7,abstract:null,book:{id:"673",slug:"dyslexia-a-comprehensive-and-international-approach",title:"Dyslexia",fullTitle:"Dyslexia - A Comprehensive and International Approach"},signatures:"Taeko N. Wydell",authors:[{id:"87489",title:"Prof.",name:"Taeko",middleName:"N.",surname:"Wydell",slug:"taeko-wydell",fullName:"Taeko Wydell"}]},{id:"58597",title:"Testosterone and Erectile Function: A Review of Evidence from Basic Research",slug:"testosterone-and-erectile-function-a-review-of-evidence-from-basic-research",totalDownloads:1349,totalCrossrefCites:2,totalDimensionsCites:2,abstract:"Androgens are essential for male physical activity and normal erectile function. Hence, age-related testosterone deficiency, known as late-onset hypogonadism (LOH), is considered a risk factor for erectile dysfunction (ED). This chapter summarizes relevant basic research reports examining the effects of testosterone on erectile function. Testosterone affects several organs and is especially active on the erectile tissue. The mechanism of testosterone deficiency effects on erectile function and the results of testosterone replacement therapy (TRT) have been well studied. Testosterone affects nitric oxide (NO) production and phosphodiesterase type 5 (PDE-5) expression in the corpus cavernosum through molecular pathways, preserves smooth muscle contractility by regulating both contraction and relaxation, and maintains the structure of the corpus cavernosum. Interestingly, testosterone deficiency has relationship to neurological diseases, which leads to ED. Testosterone replacement therapy is widely used to treat patients with testosterone deficiency; however, this treatment might also induce some problems. Basic research suggests that PDE-5 inhibitors, L-citrulline, and/or resveratrol therapy might be effective therapeutic options for testosterone deficiency-induced ED. Future research should confirm these findings through more specific experiments using molecular tools and may shed more light on endocrine-related ED and its possible treatments.",book:{id:"5994",slug:"sex-hormones-in-neurodegenerative-processes-and-diseases",title:"Sex Hormones in Neurodegenerative Processes and Diseases",fullTitle:"Sex Hormones in Neurodegenerative Processes and Diseases"},signatures:"Tomoya Kataoka and Kazunori Kimura",authors:[{id:"219042",title:"Ph.D.",name:"Tomoya",middleName:null,surname:"Kataoka",slug:"tomoya-kataoka",fullName:"Tomoya Kataoka"},{id:"229066",title:"Prof.",name:"Kazunori",middleName:null,surname:"Kimura",slug:"kazunori-kimura",fullName:"Kazunori Kimura"}]}],onlineFirstChaptersFilter:{topicId:"18",limit:6,offset:0},onlineFirstChaptersCollection:[{id:"82319",title:"Traumatic Optic Neuropathy",slug:"traumatic-optic-neuropathy",totalDownloads:2,totalDimensionsCites:0,doi:"10.5772/intechopen.104731",abstract:"Traumatic optic neuropathy (TON) is a specific neurological sequence of traumatic brain injury (TBI). It has a different mechanism than other most neurologic complications of head trauma and its consequences can be devastating. The damage can be from direct penetrating trauma or bone fracture injuring the optic nerve directly or secondary to indirect blunt trauma (usually causing traction). The diagnosis of TON is based on the clinical history and examination findings indicative of optic neuropathy, especially the presence of defective pupillary light response. TON can cause only mild vision loss but, in some cases, severe vision loss is present. Imaging findings can support the diagnosis, and provide information on the mechanism as well as treatment options. The treatment options include observation alone, systemic steroids, erythropoietin, surgical decompression of the optic canal, or combination. The evidence base for these various treatment options is controversial and each treatment has its side effects and risks. Poor prognostic factors include poor visual acuity at presentation, loss of consciousness, no improvement in vision in the first 48 hours, and evidence of optic canal fractures on neuroimaging.",book:{id:"11367",title:"Traumatic Brain Injury",coverURL:"https://cdn.intechopen.com/books/images_new/11367.jpg"},signatures:"Ainat Klein and Wahbi Wahbi"},{id:"82203",title:"Resting-State Brain Network Analysis Methods and Applications",slug:"resting-state-brain-network-analysis-methods-and-applications",totalDownloads:19,totalDimensionsCites:0,doi:"10.5772/intechopen.104827",abstract:"Resting-state fMRI has been widely applied in clinical research. Brain networks constructed by functional connectivity can reveal alterations related to disease and treatment. One of the major concerns of brain network application under clinical situations is how to analyze groups of data to find the potential biomarkers that can aid in diagnosis. In this paper, we briefly review common methods to construct brain networks from resting-state fMRI data, including different ways of the node definition and edge calculation. We focus on using a brain atlas to define nodes and estimate edges by static and dynamic functional connectivity. The directed connectivity method is also mentioned. We then discuss the challenges and pitfalls when analyzing groups of brain networks, including functional connectivity alterations, graph theory attributes analysis, and network-based statistics. Finally, we review the clinical application of resting-state fMRI in neurorehabilitation of spinal cord injury patients and stroke patients, the research on the mechanism and early diagnosis of neurodegenerative diseases, such as multiple system atrophy, as well as the research on brain functional network alteration of glioma patients.",book:{id:"11742",title:"Neurophysiology",coverURL:"https://cdn.intechopen.com/books/images_new/11742.jpg"},signatures:"Yunxiang Ge and Weibei Dou"},{id:"82099",title:"Recent Advances in the Development of Biofluid-Based Prognostic Biomarkers of Diffuse Axonal Injury",slug:"recent-advances-in-the-development-of-biofluid-based-prognostic-biomarkers-of-diffuse-axonal-injury",totalDownloads:13,totalDimensionsCites:0,doi:"10.5772/intechopen.104933",abstract:"Even though head injury is a silent pandemic of the century producing immense social and economic impact, predictive models have not been established to develop strategies promoting the development of reliable diagnostic tools and effective therapeutics capable of improving the prognosis. Diffuse axonal injury (DAI) is a type of traumatic brain injury (TBI) that results from a blunt injury to the brain. Discovering biomarkers for DAI have been a matter of debate and research. A number of studies have reported biomarkers that are correlated with severity of TBI but no conclusive and reproducible clinical evidence regarding the same has been put forward till now. Additionally, many DAI biomarkers have limitations so that they cannot be generalized for universal applications. The properties of these biomarkers should be extensively researched along with the development of novel biomarkers to aid important clinical decisions for the benefit of the society. This chapter summarizes the existing biofluid-based biomarkers, critically examines their limitations and highlights the possibilities of a few novel biomolecules as prognostic biomarkers of DAI.",book:{id:"11367",title:"Traumatic Brain Injury",coverURL:"https://cdn.intechopen.com/books/images_new/11367.jpg"},signatures:"Vinu V. Gopal, Rinku Raj Mullasseril and Goutam Chandra"},{id:"81998",title:"Understanding the Neuropathophysiology of Psychiatry Disorder Using Transcranial Magnetic Stimulation",slug:"understanding-the-neuropathophysiology-of-psychiatry-disorder-using-transcranial-magnetic-stimulatio",totalDownloads:5,totalDimensionsCites:0,doi:"10.5772/intechopen.103748",abstract:"Transcranial magnetic stimulation (TMS) is a safe and non-invasive tool that allows researchers to probe and modulate intracortical circuits. The most important aspect of TMS is its ability to directly stimulate the cortical neurons, generating action potentials, without much effect on intervening tissue. This property can be leveraged to provide insight into the pathophysiology of various neuropsychiatric disorders. Using multiple patterns of stimulations (single, paired, or repetitive), different neurophysiological parameters can be elicited. Various TMS protocol helps in understanding the neurobiological basis of disorder and specific behaviors by allowing direct probing of the cortical areas and their interconnected networks. While single-pulse TMS can provide insight into the excitability and integrity of the corticospinal tract, paired-pulse TMS (ppTMS) can provide further insight into cortico-cortical connections and repetitive TMS (rTMS) into cortical mapping and modulating plasticity.",book:{id:"11742",title:"Neurophysiology",coverURL:"https://cdn.intechopen.com/books/images_new/11742.jpg"},signatures:"Jitender Jakhar, Manish Sarkar and Nand Kumar"},{id:"81646",title:"Cortical Plasticity under Ketamine: From Synapse to Map",slug:"cortical-plasticity-under-ketamine-from-synapse-to-map",totalDownloads:17,totalDimensionsCites:0,doi:"10.5772/intechopen.104787",abstract:"Sensory systems need to process signals in a highly dynamic way to efficiently respond to variations in the animal’s environment. For instance, several studies showed that the visual system is subject to neuroplasticity since the neurons’ firing changes according to stimulus properties. This dynamic information processing might be supported by a network reorganization. Since antidepressants influence neurotransmission, they can be used to explore synaptic plasticity sustaining cortical map reorganization. To this goal, we investigated in the primary visual cortex (V1 of mouse and cat), the impact of ketamine on neuroplasticity through changes in neuronal orientation selectivity and the functional connectivity between V1 cells, using cross correlation analyses. We found that ketamine affects cortical orientation selectivity and alters the functional connectivity within an assembly. These data clearly highlight the role of the antidepressant drugs in inducing or modeling short-term plasticity in V1 which suggests that cortical processing is optimized and adapted to the properties of the stimulus.",book:{id:"11374",title:"Sensory Nervous System - Computational Neuroimaging Investigations of Topographical Organization in Human Sensory Cortex",coverURL:"https://cdn.intechopen.com/books/images_new/11374.jpg"},signatures:"Ouelhazi Afef, Rudy Lussiez and Molotchnikoff Stephane"},{id:"81582",title:"The Role of Cognitive Reserve in Executive Functioning and Its Relationship to Cognitive Decline and Dementia",slug:"the-role-of-cognitive-reserve-in-executive-functioning-and-its-relationship-to-cognitive-decline-and",totalDownloads:30,totalDimensionsCites:0,doi:"10.5772/intechopen.104646",abstract:"In this chapter, we explore how cognitive reserve is implicated in coping with the negative consequences of brain pathology and age-related cognitive decline. Individual differences in cognitive performance are based on different brain mechanisms (neural reserve and neural compensation), and reflect, among others, the effect of education, occupational attainment, leisure activities, and social involvement. These cognitive reserve proxies have been extensively associated with efficient executive functioning. 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