Vitamin-K is a demanding multi-functional health product in the market and belongs to a class of isoprenoid molecules that comprises methylnaphthoquinone (MK) unit attached to an isoprene side chain. They are fat soluble and differ in the extent of side chain & obtained in the nature as vitamin K1 (phylloquinone), menaquinone/vitamin K2, and other lipoquinones. Owing to their owned polyprenyl side chain, they are hydrophobic/lipophilic in nature. Generally, the synthesis of vitamin K and its variants suffers with isomerization (for example 11 isomers were identified for cis/trans MK-7). Naturally, in bio-systems vitamin K produces through shikimic acid pathway and terpene biosynthetic pathway for the synthesis of menaquinone part & prenyl side chain parts respectively. Menadione or its auxiliaries are commonly being used as substrates to the synthesis of vitamin K variants through the involvement of condensation reactions, Friedel-Craft alkylation’s, Claisen rearrangement, Diels-Alder reactions and others. Importantly, organometallic reagents, such as Grignard, Gilman, organotelluride and other reagents could be the promising and consistent choice of substrate to the synthesis of various vitamin K’s. Vitamin K is well known for blood coagulation. As an antihaemorrhagic vitamin, it’s also being the current interest for the treatment of bone and vascular diseases. In addition, vitamin k is indispensable for the activation of vitamin K dependent (VKD) proteins and that are present almost in all tissues and responsible for hemostasis, bone mineralization, arterial calcification, apoptosis, phagocytosis, growth control, chemotaxis, and signal transduction. This chapter summarizes various synthetic approaches of vitamin K & derivatives and their biological functions.
- Vitamin K
- Synthetic auxiliaries
- Organometallic reagents
- Vascular diseases
Vitamin K is a family of natural products, comprises vitamin K1 (phylloquinone), vitamin K2 (methylnaphthoquinones/menaquinones-MK) and vitamin K3 (menadione). These are structurally methylated napthoquinones possess isoprene side chain (vitamin K1 & vitamin K2) and they vary in the extent of isoprene side chain, in terms of number of isoprene units and its level of saturation [1, 2, 3, 4, 5]. However, these structural changes are apparently simple, but needs exist in its specific stereochemistry to exhibit their biological functions. These are the fat-soluble compounds and pertains to a class of lipoquinones . Vitamin K2 are varied on the basis of number of isoprene units present in the side chain and they denoted as MK-n (n – number of isoprene units; Figure 1). The vitamin K2 if contains saturated isoprene units referred as MK-n(m-H2), wherein m is a roman numeral represents isoprene unit number in the side chain which underwent reduction. Among the two natural forms of vitamin K, vitamin K1 presents in green leafy vegetables, for example, kale, collard greens, turnip greens, iceberg lettuce, broccoli, spinach, and brussels sprouts. The Vitamin K2 presents naturally in eggs, meet, fermented foods (natto, cheese, yogurt and sauerkraut) also present in bacteria, for example, MK-9 is found in mycobacterium with nine isoprene units and also its reduced derivative at second isoprene unit MK-9(II-H2) could be active as electron transport agent in [3, 7]. In humans, these menaquinones display several biological properties, including facilitating blood coagulation [8, 9]. In bacteria, these molecules assisting the synthesis of ATP through transport of electrons between the membrane-bound protein complexes and thus acting as electron acceptors and donors in the respiratory electron transport [10, 11]. Vitamin K and its analogues could not synthesize by mankind/animals. So, its required to supply an adequate amount through the dietary sources.
2. Vitamin K biosynthesis
Naturally, bacteria producing different variants of vitamin K. Among the aerobic bacteria, most of its Gram-negative bacteria contain ubiquinone as the sole quinone, whereas the menaquinone is the only quinone presents in aerobic Gram-positive bacteria. But, in the case of anaerobic bacteria, irrespective of whether it is Gram-positive or Gram-negative, it produces benzoquinones (ubiquinone), naphthoquinones (menaquinones; MK-n), demethylmenaquinones (DMK-n) . The Gram-negative bacteria, such as,
These menaquinones have prenyl side chains with all-
Cox and Gibson discovered in 1964, shikimate was present in menaquinone of
Shikimate pathway for the synthesis of menaquinones is presented in Figure 4. Shikimate was proposed to converted initially into chorismite before its incorporation into menaquinone . In
3. Various synthetic approaches to vitamin K
In 1939, Fieser , Binkley , and Almquist  were reported initially the synthesis of vitamin K1 independently. The condensation reaction of either menadione/2-methyl-1,4-naphthohydroquinone with natural phytol in the presence of oxalic acid or zinc dust in acetic acid produce vitamin K1 (Figure 5).
The initial synthetic approaches were generally proceeding through the usage of Friedel-Craft alkylation’s for introducing the side chains through its coupling to menadions, which led to the generation of mixture of isomers at the Δ2 position and produced
In addition, Organocuprates were also being used to the synthesis of vitamins K’s. Menaquinone and phylloquinone are synthesized by Chenard group, from the reaction of Gilman based bisketals of quinone substrate with allyl halides and it afforded high yields and good stereoselectivity at the Δ2 position of the vitamin K (Figure 7). The quinone bromide underwent metalation to produce the corresponding cuprate, and its reactivity was varied with different electrophilic substrates (RX). Among the tested electrophilic substrates, if the electrophilic group is small enough (allyl bromide) then the two alkyl groups of cuprate reagent were being used being transferred in the reaction. If the reaction with bulkier electrophilic halide (for example benzyl chloride/bromide, cyclohexanecarbonyl acid chloride), cuprate can transform only one alkyl group . Syper group synthesized protected forms of MK-1 and MK-2 in appreciable yields through the coupling reaction of prenyl bromide and geranyl bromide with 2-bromo-3-methyl-1,4-dimethoxynaphthalene . Generally, these organometallics (Grignard reagents, organocuprates and organolithiums) mediated synthesis of vitamin K required the usage of protected quinones to avoid the side reactions. Unprotected quinones could also give the vitamin k synthesis through their direct coupling with organostannates  and organisilanes .
Tso and group developed an efficient and conceptually distinguished one-pot protocol to the synthesis of vitamin k, wherein the 3-substituted isobenzofuranone was treated with a base, the generated quinone methide which underwent an anionic [4 + 2] cycloaddition reaction with the alkenyl phenyl-sulfone (dienophile) and that was being synthesized from the corresponding allyl phenylsulfone and various prenyl bromide (RBr). Finally, the vitamin K was produced by the elimination of sulfone from the intermediate. This method was very compatible to the synthesis of phylloquinone and different menaquinone variants MK-1, MK-2 and MK-9 about 60–65% yields (Figure 8) .
In 2015, Mal et al., extended this protocol to the synthesis of menadione derivatives (MK-n molecules) by a base mediated reaction of 3-substituted phthalide with methyl methacrylate . The yield of the reaction was verified in this reaction with various leaving groups, such as - methoxy, phenyl sulfonyl, isonitrile, thiophenyl, and nitrile. The nitrile leaving group in the substrate was found to be the best suitable one for the reaction. The 3-nitrilephthalide is to be a menadione auxiliary to the reaction of MK-n variants (Figure 9). The reaction was proceeds through a base mediated anionic driven annulation of 3-nucleofugal phthalides with α-alkyl/aryl acrylates followed by demethoxycarbonylation. If the polyprenyl acrylates are to be the substrates, then various analogs of menadione were being produced (MK-n).
Side chain functionalization methods were also being developed to the synthesis of vitamin K analogs. These derivatized vitamin K are received a great deal of interest to reveal the structure activity relationship studies (SAR). These analogs were also proved to be as inhibitors of vitamin K dependent carboxylase and vitamin K epoxide reductase . The side chain stereochemistry is very essential to exhibit the biological activity, as the
Snyder and group during their sustained efforts to retain the stereochemistry of
However, these enolyte alkylations were successful but not very practical. To accomplish the practical methods, Snyder group developed transmetallation method for the synthesis of vitamin K [ 38]. Lithium, magnesium and copper had used to convert 2-bromomenadiol to its corresponding metallo derivative. As an electrophilic partner authors chosen initially aldehydes, but aldehydes are unsuccessful as the resultant alcohol functionality removal afforded either vinyl alkenes or α-isoprene double bond isomerization. Later, the prenyl halides are found to be the appropriate substrates of electrophiles for these transformations. Primarily, reactions were performed to evaluate the stereoretention of the α-isoprene double bond in each of the 2-metallo derivatives. As per their expectation, all these metallo derivatives could not affect the configuration of the isoprene component of the vitamins. It was found that, organo magnesium reagents are more efficient than organocuprates and that in turn efficient than organo lithium reagents. Among the prenyl halides, prenyl bromides are generated excellent yields of menaquinone analogs (Figure 11). The coupling of 2-magnesio or 2-cupro-3-methyl-l,4-dimethoxynaphthalene with geranyl bromide produced in >90% yield. In this method, MK-9 was obtained in 73% yield by treating solanesyl bromide with menadiol.
In the various synthetic strategies to the vitamin K in the literature, Friedel-Crafts alkylation protocol of menadiols is the most popular across the literature. Hirschmann group developed a method wherein the applicability of various Lewis and Bronsted-Lowry acids were being used for the synthesis of vitamin K1 . Monoacetate of menadiol substrate was treated with phytol in presence of various acids. Among, the acids, BF3.Et2O afforded appreciable yields (67%) rather others being tested KHSO4, oxalic acid and Duolite C-60. After Friedel-Crafts alkylation, finally, the acetate group was removed by the treatment with Ag2O (Figure 12).
Later, in 1990 Schmid and group enriched the yield of the reaction by treating the reaction of menadiol monoacetate with phytyl chloride in presence of a base potassium carbonate, wherein O-phytylated derivative had obtained. This was further undergoing Claisen rearrangement under acidic treatment with catalytic amount of BF3.Et2O to C-phytylated product in 76–80% yield and the ratio of the configurations of E and Z isomers was found to be 97:3. The deacetylation was carried out in basic medium (Figure 13). Vitamin K1 obtained in this procedure is to be 96.5% and with E-configuration, Z -configuration compound was with very low yield (3%) .
Apart from the electrophilic side chain attachment to the menadiones, radicals also found to do the same work to make the synthesis of vitamin K. Jacobson and coworkers in 1972, developed a method to the alkylation of quinones by the use of radicals and that were produced by metal/persulphate catalyzed decorboxylation of the corresponding carboxylic acid. 4-Methyl-3-pentenoic acid produced the 3,3-dimethylallyl radical while in presence of AgNO3, (NH4)2S2O8. Initially Ag+ is reacted with S2O8−2 to form Ag+2 and which abstract an electron from carboxylic acid to produce allyl radical by the ejection of CO2. This allyl radical resonates and unexpectedly less stable isoform γ,γ-dimethylallylquinone was given the product instead to α,α-dimethylallylquinone (Figure 14). By this procedure MK-1 obtained in 70% yield as a γ,γ-dimethylallylquinone as to hold a stable alkene .
Later, Yamago et al., found the applicability of organotellurium compounds to the radical coupling reactions with a variety of quinone substrates . This method could offer the general protocol to the synthesis of polyprenyl menadiones with complete retention of stereochemistry. Organotellurides were prepared by SmI2 mediated coupling reaction of its corresponding bromides with ditollyl ditelluride (Tol)2Te2. This telluride later produced prenyl radical under photo/thermal energy source and that interact with menadione to produce respective MK-n related to the length of the prenyl side chain. While geranyl tolyl telluride (73:23 mixture of the trans and cis isomers) react with 2-methyl-1,4-naphthoquinone to produce MK-2 in moderate yield. The stereochemistry of the product (7:3) informed that the reaction gave the retention of stereo chemistry of organic telluride in to the product (Figure 15).
Diels-Alder reactions were useful to construct napthoquinine structural unit, as Rüttimann and group being used this concept to develop protocol for the synthesis of vitamin K1. The reaction of dihydroisobenzofurane was performed with activated alkyne dienophile (96:4 E/Z) at 80°C overnight to form the trimethyl sillyl ether of Diels-Alder adduct and its reaction further carried out with methanol and then methyl group at C2 position is achieved through the reduction of ester with bis(2-methoxyethoxy)aluminum hydride followed by air oxidation produces vitamin K in moderate yield (Figure 16a, 50%) . During the reaction the isoprene double bond configuration was not altered. Later, he developed the reaction by taking an auxiliary to support the reaction. Rüttimann along with Büchi had taken cyclopentadiene as a substrate auxiliary and its reaction was carried out with menadione to generate Diels-Alder adduct (Figure 16b). Prenylation/ alkylation at C3 position of Diels-Alder adduct was performed under strong base (KO
Inspired by menadione auxiliary applicability in the synthesis of vitamin K1, Battula, S., and group, thought to introduce the polyprenyl side chain on to the menadione to synthesis MK-n variants. This menadione surrogate was utilized to the synthesis of MK-9 in one pot protocol. The reaction of 1-Chloro-
In the view of the requirement of MK-7 owing to its high lipophilicity and good bioavailability in small intestines and about 3 days half-life than compare to other menaquinones and ubiquinone, Aneta et al. in 2016 developed a practical synthetic strategy for vitamin K2 (MK-7) . The synthesis of MK-7 was achieved in all the
Among the two coupling components, hexaprenyl fragment was obtained from commercially available
As this method was convenient in the availability of starting substrates and perfect stereochemistry of the product, Battula, S., and group developed the similar convergent synthetic strategy to MK-6 as well (vitamin K2 variant) in all the trans forms of side chain through “1+5 convergent synthetic approach” of pentaprenyl chloride with monoprenyl menadione derivative . During this survey, authors found that bromo based polyprenyl substrate was produced S
Lipshutz and group developed a method to introduce a one carbon handle at the C-3 position of menadione molecules and it offered a good synthetic protocol to a wide range of MK-derivatives through a highly probable
In the early of 1900s, Saa and group successfully established a protocol by which aldehydes were being used as electrophiles to launch the prenyl side chains in to menadione molecules in the production of MK-2 and MK-4 . During this protocol, bismethyl ether of 2-bromomenadiol undergoes reaction with
4. Brief discussion of vitamin K biology
Biologically these molecules are very important and have been reported for several biomedical purposes. Owing to their severe liphophilicity/ hydrophobicity due to containing multiple isoprene units in the side chain, they are with less solubility and thus causes to difficulties to assess in-vitro studies as these are performed in aqueous solutions. These napthoquinones have been displayed promising biological activities against tubercular [60, 61], cancer [62, 63, 64], cardiovascular [65, 66] and diabetes [67, 68].
In mycobacteria, MenJ (
As a known fact that vitamin K is an essential nutrient that displays potential anticancer properties on a variety of tumor cells [71, 72]. Quinones are the important natural and synthetic molecules as they have considerable biological potential. These compounds are display antitumor activity through several mechanism of action. Generally, these molecules have problems with respect to solubility, stability and toxicity. Owing to this reason, these molecules are using as drugs through alternative procedures like controlled-release system of these quinones, and it could be a strategy for improving the pharmacological profile of this class of compounds. Vitamin K mediated mechanisms proceeds to prevent the cell proliferation and growth although unclear, but mostly through oxidative effect and direct arylation of thiols may deplete glutathione and cell cycle arrest. The quinone structure in vitamin k is responsible for the modulation of redox-balance and induction of oxidative stress in cancer cells. The anticancer properties of vitamin K1 and vitamin K2 mostly mediated by non-oxidative mechanisms, probably through transcription factors, but vitamin K3 does by reducing oxidative stress and arylation at higher concentrations. It’s been evidenced that, bulk doses of vitamin K2 (2.5 grams given per day) could be safe and not caused to enhancing toxicity levels . Vitamin K2 also prevents hepatocarcinogenesis in patients with hepatic cirrhosis . Quinones generally undergo one-electron and two-electron reductions, leads to produce semiquinone radicals, as well as hydroquinone’s respectively. These factors reduce oxidative stress through the consumption of superoxide radicals and cause to cancer cell homeostasis.
Vitamin K known to reduce complications and improve clinical issues of pre-diabetes and diabetes. Type-2 diabetes mellitus (T2DM) demonstrates when pancreatic
Vitamin K2 also useful to the bone and cardiovascular related problems. As we know, lower intake of calcium can decrease the bone mineral density, and thus can increase the risk of bone fractures. Although supplemental calcium helps to enhance bone mineral density and strength (prevent osteoporosis), recent evidences informed that higher consumption of calcium supplements may lead to the risk for heart diseases and also can cause to accelerated deposit of calcium in blood-vessel walls and soft tissues. While the vitamin K2 is related with the inhibition of arterial calcification and arterial stiffening, which means that increased vitamin K2 intake could be lower the risk of vascular damage as it activates matrix GLA protein (MGP), and that inhibits the deposits of calcium on the walls, and thus reducing the health risks that are associated with calcium levels [77, 78]. The essential component to the synthesis of Gla-protein family is vitamin K and that is very important to the hemostasis as its deficiency causes to acute and dangerous condition due to excessive bleeding.
This chapter summarized various synthetic approaches of different variants of vitamin K and their biological application. Although several methods are available, but menadione and its substrate auxiliaries (for example, Diels-Alder adducts, organometallic compounds and others) are the choices to the synthesis of vitamin K’s. Their synthesis proceeds through several reactions like, Friedel-Craft alkylation’s, condensation reactions, Claisen rearrangement, Diels-Alder reactions and others and by the involvement of nucleophilic and free radical reactions. It also included the information regarding their natural sources. As the huge importance of vitamin K to the mankind, it is being used as food supplementation because it’s not produced by mankind. The major dietary source of vitamin K is phylloquinone, which is synthesized by plants and algae. Vitamin K2 (various forms of menaquinone; MK-4 to MK14), produces from bacteria in the human gut and plays a lesser role in the provision of vitamin K, since it is taken up by the body to only a limited extent. In infants the development of vitamin K is very low, due to its deficiency they are offering vitamin K immediately after the birth and the initial days life. Also incorporated the utility of vitamin K, as its great role in the blood coagulation, in the maintenance of bone health and healthy nervous system, prevention of cardio vascular disease and diabetes.
Menadione, a synthetic product and being used as a pharmaceutical interested molecule. Menaquinone-4 mainly resides in the brain tissues and generates from a tissue-specific transformation of vitamin K. The metabolism of vitamin K is an essential factor to study further vitamin K biology. Further knowledge in this context of vitamin K proved to be beneficial in many areas of science for example like medicine .
Uka Tarsadia University, Bardoli, Gujarat, India.
Conflict of interest
The authors declare no conflict of interest.
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