Specification of grazing carried out by the Centre of Regional Produce in Koniakow.
\r\n\tMany tried to define it, and its definition is always related to those who are in power, that being explained by the fact that this power and the abuse of it precisely, gives the access to being corrupted and practicing the acts that fall under corruption.
\r\n\r\n\tWe can find various types of corruption such as bribery, lobbying, extortion, cronyism, nepotism, parochialism, patronage, influence peddling, graft, and embezzlement. Also giving or accepting bribes or inappropriate gifts, double-dealing, under-the-table transactions, manipulating elections, diverting funds, laundering money, and defrauding investors.
\r\n\tNo government is immune to corruption. According to the World Bank, “the causes of corruption are always contextual, rooted in a country's policies, bureaucratic traditions, political development, and social history”.
\r\n\tThis indeed has consequences for increasing inequality, impacts government expenditure and services, shadow economy, and crime.
\r\n\tThis book will be a collection of chapters on Corruption. It welcomes contributions related to the nature of corruption its types and how corruption is undertaken in a certain context and the ways to deal with corruption will be part of this book. We value including materials on Corruption in organizations and ways to solve it. The origins of corruption and the way to deal with corruption, how to provide solutions, and any new insights on corruption will be part of this book.
",isbn:"978-1-80356-696-2",printIsbn:"978-1-80356-695-5",pdfIsbn:"978-1-80356-697-9",doi:null,price:0,priceEur:0,priceUsd:0,slug:null,numberOfPages:0,isOpenForSubmission:!0,isSalesforceBook:!1,hash:"9cda6d2feaa52a6d523da74f2e2d7ffb",bookSignature:"Dr. Josiane Fahed-Sreih",publishedDate:null,coverURL:"https://cdn.intechopen.com/books/images_new/11772.jpg",keywords:"Corruption, Origins, Types, Corporate Governance, Organizational Performance, Solutions, Corruption Index, Private Sector, Lebanon, Accountability, Anti-corruption, Public Policy",numberOfDownloads:null,numberOfWosCitations:0,numberOfCrossrefCitations:null,numberOfDimensionsCitations:null,numberOfTotalCitations:null,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"March 23rd 2022",dateEndSecondStepPublish:"April 20th 2022",dateEndThirdStepPublish:"June 19th 2022",dateEndFourthStepPublish:"September 7th 2022",dateEndFifthStepPublish:"November 6th 2022",remainingDaysToSecondStep:"a month",secondStepPassed:!0,currentStepOfPublishingProcess:3,editedByType:null,kuFlag:!1,biosketch:"Dr. Fahed-Sreih is the director of the Institute of Family and Entrepreneurial Business and a chairperson in the Department of Management. She obtained a Ph.D. from Sorbonne University, France, and received the 2007 FFI International Award for outstanding achievement in furthering the understanding of family business issues between two or more countries. She is on the editorial board of the Journal of Family Business Management and a keynote speaker for corporate governance conferences.",coeditorOneBiosketch:null,coeditorTwoBiosketch:null,coeditorThreeBiosketch:null,coeditorFourBiosketch:null,coeditorFiveBiosketch:null,editors:[{id:"103784",title:"Dr.",name:"Josiane",middleName:null,surname:"Fahed-Sreih",slug:"josiane-fahed-sreih",fullName:"Josiane Fahed-Sreih",profilePictureURL:"https://mts.intechopen.com/storage/users/103784/images/system/103784.jfif",biography:"Dr. Josiane Fahed-Sreih is a full-time associate professor of Management in the School of Business, Lebanese American University. She is the founder and director of the Institute of Family and Entrepreneurial Business and a chairperson in the Department of Management at the same university. She was previously the assistant dean. She obtained a Ph.D. from Sorbonne University, Paris, France. Dr. Fahed-Sreih is the Middle East Coordinator for the Family Firm Institute (FFI), the USA, and a family wealth and family business consultant. She received the 2007 FFI International Award for outstanding achievement in furthering the understanding of family business issues that occur between two or more countries. She has participated in and organized international conferences, workshops, and seminars. She has presented at major conferences locally and internationally and consulted on management issues in many countries, including Saudi Arabia, Dubai, Jordan, Qatar, Kuwait, Syria, Bahrain, Oman, France, Cyprus, and Lebanon. She currently sits on five boards of directors as a shareholder, two as a chairman of the board, and one as an independent director in the private sector. She is also an advisor on boards of community service organizations. \n\nShe speaks regularly to trade and professional groups and presents her research at academic conferences worldwide. She is frequently invited as a keynote speaker to the recognized family business and corporate governance conferences. Her research interests are in management, family business, the functioning of boards of directors, and corporate governance. She has published three books, several book chapters, and academic articles in international journals. 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Venkateswarlu",coverURL:"https://cdn.intechopen.com/books/images_new/371.jpg",editedByType:"Edited by",editors:[{id:"58592",title:"Dr.",name:"Arun",surname:"Shanker",slug:"arun-shanker",fullName:"Arun Shanker"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"72",title:"Ionic Liquids",subtitle:"Theory, Properties, New Approaches",isOpenForSubmission:!1,hash:"d94ffa3cfa10505e3b1d676d46fcd3f5",slug:"ionic-liquids-theory-properties-new-approaches",bookSignature:"Alexander Kokorin",coverURL:"https://cdn.intechopen.com/books/images_new/72.jpg",editedByType:"Edited by",editors:[{id:"19816",title:"Prof.",name:"Alexander",surname:"Kokorin",slug:"alexander-kokorin",fullName:"Alexander Kokorin"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"314",title:"Regenerative Medicine and Tissue Engineering",subtitle:"Cells and Biomaterials",isOpenForSubmission:!1,hash:"bb67e80e480c86bb8315458012d65686",slug:"regenerative-medicine-and-tissue-engineering-cells-and-biomaterials",bookSignature:"Daniel Eberli",coverURL:"https://cdn.intechopen.com/books/images_new/314.jpg",editedByType:"Edited by",editors:[{id:"6495",title:"Dr.",name:"Daniel",surname:"Eberli",slug:"daniel-eberli",fullName:"Daniel Eberli"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"57",title:"Physics and Applications of Graphene",subtitle:"Experiments",isOpenForSubmission:!1,hash:"0e6622a71cf4f02f45bfdd5691e1189a",slug:"physics-and-applications-of-graphene-experiments",bookSignature:"Sergey Mikhailov",coverURL:"https://cdn.intechopen.com/books/images_new/57.jpg",editedByType:"Edited by",editors:[{id:"16042",title:"Dr.",name:"Sergey",surname:"Mikhailov",slug:"sergey-mikhailov",fullName:"Sergey Mikhailov"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"1373",title:"Ionic Liquids",subtitle:"Applications and Perspectives",isOpenForSubmission:!1,hash:"5e9ae5ae9167cde4b344e499a792c41c",slug:"ionic-liquids-applications-and-perspectives",bookSignature:"Alexander Kokorin",coverURL:"https://cdn.intechopen.com/books/images_new/1373.jpg",editedByType:"Edited by",editors:[{id:"19816",title:"Prof.",name:"Alexander",surname:"Kokorin",slug:"alexander-kokorin",fullName:"Alexander Kokorin"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}}]},chapter:{item:{type:"chapter",id:"78336",title:"The Pastoralism in the Silesian Beskids (South Poland): In the Past and Today",doi:"10.5772/intechopen.99722",slug:"the-pastoralism-in-the-silesian-beskids-south-poland-in-the-past-and-today",body:'The Silesian Beskids belong to the Carpathian Mountains, the second-longest mountain range in Europe. The system forms an arc throughout Central and Eastern Europe and stretches from the far eastern Czech Republic in the north-west, through Slovakia, Poland, Hungary, Ukraine, Romania to Serbia in the south. The range is roughly 1500 km long and covers the area of about 210 000 km2.
The Silesian Beskids are located in the westernmost part of the Carpathians, in the border zone of the Czech Republic, Slovakia and Poland (Figure 1). The area borders with the Żywiec Basin in the east, the Żywiec Beskids in the south-east, the Little Beskids in the north-east and the Silesian Foothills in the north. The range covers an area of 561 km2 and embraces two main ranges separated by the Vistula valley. The height of individual peaks ranges from 800 to 1250 m a.s.l. The highest peak, Skrzyczne, is 1257 m high [1].
Location of the Silesian Beskids in the Polish Carpathians.
The Beskids are the mid-height mountains, characterised by extensive ridge forms with numerous flattening and deep valleys, with the longitudinal course (Figure 2). Their shapes result mainly from the geological structure which is dominated by the Carpathian flysch, i.e. alternately arranged layers of sandstone, conglomerates and slate.
Silesian Beskids landscape; (a) autumn; (b) winter.
The Silesian Beskids are located in the Carpathian climatic zone, in a temperate climate, shaped by polar and sea air masses. The climate is typical for medium-sized mountains and is characterised by low average air temperature, high precipitation and strong winds. The air temperature is determined by altitude, exposure and inclination of slopes. For the altitudes below 650 m, the average annual temperature is 6–8°C [2]. As the altitude increases, the annual air temperature drops by 0.5 for every 100 m. Temperature on the southern slopes is 1°C higher than on the northern slopes. The average annual rainfall is above 1350 mm, which is one of the highest in Poland and higher compared to other parts of the Beskids [3]. The high precipitation results from the area protruding westwards and its greater exposure to rain-bearing winds from NW and SW directions. In the autumn, as the temperature drops, the precipitation changes into snowfall. The first snowfalls are recorded in October and can sometimes last until May. The number of days with snowfall varies greatly year to year and may amount up to even 200 days in the year. The snow cover remains for many months and in certain years it may be even 2 m thick. In many years snow cover stays for a long time in landslide depressions or densely forested places, which delays the growing season [4]. Climatic and physiographic conditions, i.e. high annual rainfall, heavy snowfall, significant inclination of the slopes and poor permeability of the flysch result in a formation of a dense river network (Figure 3). There are numerous springs including springs of the Vistula - the largest Polish river [5].
Typical mountain streams flowing down the peaks.
Due to harsh climatic conditions, short vegetation period, steep slopes prone to water erosion, and lack of fertile agricultural land, the Beskids were uninhabited for a long time. The first settlers from the lowlands appeared in the region only in the 13th century [6]. The newcomers settled in the Beskid valleys, cultivated undemanding crops, mainly: rye, barley, flax, peas, and grazed cattle on lower-lying meadows.
About one-two centuries later, the Wallachian tribes appeared in the region. Their ethnic affiliation is not clear [7, 8]. In the Middle Ages “Wallachians” or “Vlachs” was the name used for pastoral people from the southern Balkans who led a semi-nomadic lifestyle. In the 13th century, the Wallachians started migration from the south, along the Carpathian Mountains. They continued until the 16th century, heading northwest up to the Silesian Beskids [9, 10, 11, 12].
For several reasons, mainly economic and military, the Wallachian settlers could be attractive for local rulers. They were masters in mountain pastoralism and brought their pastoral know-how to new places [7]. Implementation of their ideas on sheep, goats and the specific breed of cattle grazing on mountain pastures and forests changed the social and economic reality in the vast area of the Carpathian Mountains [8, 12]. Sheep grazing in particular enriched the production profile and changed empty and less fertile mountain areas into lively pastoral settlements. The new activity created new tax collection opportunities for the land owners and generated higher income from the land. The Wallachians were not only experienced shepherds but also good artisans, highly skilled in carpentry, leatherwork and blacksmithing and were known to introduce and popularise innovative technique on rennet cheese manufacturing [7, 10, 11]. These innovations were easily transferred to and implemented by the local inhabitants. Together with innovations, the Wallachians passed on their own culture and pastoral lifestyle with different values and customs [13]. They also performed military-guarding function and served as police and border guards, controlling and securing strategic crossings through the mountains [10, 11].
The Wallachian transhumance involved the cyclical movement of herds between summer and winter pastures. This system was based on the natural difference in climate and vegetation between valley and mountain regions and assumed stabling and supplemental feeding practised only during the cold winter season [14, 15]. Animals were moved to highlands in spring to graze the lush forage in mountain clearings and in forests. In autumn the animals were brought down to permanent settlements into mountain villages.
The pastoral system implemented and spread by Wallachians was similar to other transhumance systems practiced around the world since animals were first domesticated, adopted and developed in different mountain areas in Europe [16]. The tradition of transhumance was common to all Mediterranean countries and for human and physical reasons was fully developed in the Iberian Peninsula [17]. According to recent archaeological studies conducted in Pyrenees in Spain, the system of altitudinal movements of sheep flocks was known already a few millennia before our era [18]. It was shown that sheep flocks with shepherds wandered from the pre-Pyrenean territories to the uplands to intermediate pastures placed between 1400 m and 1700 m a.s.l. After two months, the flocks were taken to higher grazing areas located above 2000 m a.s.l. On the way back in October the sheep were kept in intermediate pastures and after several weeks of grazing were taken to the lowland pastures.
In the Silesian Beskids, the traditional transhumance was modified and adopted to the local conditions [13, 19]. According to the local system, sheep were led into the mountains in the spring so that they would not use more fertile land in the village. In the early autumn, the animals were brought to the foothills and deeper valleys, where they were grazed in lowland fields. Then, the sheep were returned to the village, to their owners. In the early spring, after the lambs were born, the flocks were again grazed first in the valleys, in lowland pastures [12, 20].
The sheep were taken to summer pastures at the end of April, shortly after the snow had melted, just after the beginning of the vegetation season (Figure 4a) [12, 18, 19, 20, 21]. In some regions the sheep were accompanied by whole families [12]. More frequently women and children stayed in the village in the permanent settlements, while the flock was put out to pastures by owners and male relatives [13]. In case of communal grazing, the sheep that belonged to various owners were brought together to form a bigger flock which was entrusted to hired shepherd called
Sheep grazing on mountain meadow; (a) summer pastures; (b) wooden enclosures.
The shepherd and his helpers led the flock into grasslands located in the highest parts of the mountains, sometimes several kilometres away from the village. They lived seasonally in the pastures in huts made from easily available natural materials, wood and stones. During the entire season
The sheep and the people stayed in the mountains for 16–23 weeks [13, 20, 22]. At the end of September, the flocks returned to the village. For the next few weeks, the sheep were taken to lowland pastures in the foothills, lower located valleys or were grazed on fields and meadows located nearby the farm as long as possible. After the onset of frost and deep snow the animals were kept inside the barns and fed with hay. During the winter, in January and February, the new-born lambs were fed indoors with their mother’s milk and hay, to help them grow quickly to be ready to leave with the flock to the summer pastures [12].
Settlements in the mountains and infertile or border areas were often established under Wallachian law
After the collapse of the pastoral economy and a period of deep crisis, a regional project
Zackel sheep, brought to Poland from the southern Europe by the Wallachian tribes, are raised in the Beskid Mountains until today [27]. Zackel is a large group of sheep, which includes several local breeds. The group is widely spread in Central-Eastern and Southern-Europe, especially in the Balkans and Carpathian Mountains [28]. It is an undemanding primitive breed and is characterised by high resistance to diseases and harsh climatic conditions. The sheep are strong and well adapted to long treks and steep, hardly-accessible mountainous pastures. Moreover, they are distinguished by their longevity, strong mothering and herding instinct. The animals are rather small in size, possess a bare head and slight convex profile with a long and thin tail (Figure 5a). Thanks to their long neck and narrow mouths the sheep can eat scant vegetation in the pastures. Rams have long spiral horns, while ewes can be either horned or polled. The height of the adult sheep is ca. 60 cm at the withers and their weight can reach the maximum of 30–35 and 45–60 kg for ewes and rams, respectively.
Mountain sheep: (a) Zackel Sheep; (b) Polish Mountain Sheep.
The sheep are covered by the open wool coat of considerable density, with long flocks falling on both sides of the trunk. The typical colour of the fleece is white. Dark spots may occasionally appear around the eyes, at the mouth and ear ends. Three types of fleece fibres form under, intermediate and guard fractions. The thick guard hair of the outer coat provide physical protection against snow, prolonged rain and injuries, while the soft undercoat ensures the necessary thermal insulation [29]. The wool collected from different animals differs considerably in terms of fibre thickness, weight ratio of fractions, and content of coarse fibres [30].
In many countries, the usefulness of Zackel sheep was repeatedly improved through cross-breeding with other more noble breeds [31, 32]. In the late 19th and the early 20th centuries, several attempts of improving Polish Zackel were undertaken. For a long time the results of the cross-breeding were unsatisfactory due to decreased immunity, reduced fertility and lower wool quality. The considerable refinement of the breed was achieved only after the Second World War by the crossbreeding of Zackel ewes with Friesian rams and Transylvanian Zackels. In this way a new breed: Polish Mountain Sheep was produced (Figure 5b).
The Polish Mountain Sheep maintained Zackel’s adaptability to steep pastures and harsh climatic conditions as well their low feed requirements, longevity, high resistance to diseases and adjustment to extensive production systems. Through the use of Friesian rams the characteristics and yield of wool were enhanced. The goat type and numerous core hair observed in Zackel sheep were displaced and the fibres of the inner coat were lengthened. Simultaneously, the wool became thinner and achieved better physical parameters [33]. Thanks to Transylvanian Zackels, certain body conformation traits were improved. Crossbreeding also led to a significant increase in body weight and milk yield. Compared to the Zackel sheep, the bodyweight for adult sheep was approximately 10 kg greater and reached 60–70 kg for rams and 45–50 kg for ewes.
The Polish Mountain Sheep is distinguished by light and harmoniously built trunk, supported by rather thin and strong limbs and a cut, narrow rump. The head is light, with a straight profile, usually horned in rams, less frequently in ewes. The sheep are covered with an open coat with a characteristic dorsal section. The coat covers the sheep’s belly, limbs at least to the ankles and head to the line of the eyes. For the majority of the sheep, the coat is white. Occasionally, brown or black colour variations are encountered. The fleece forms double coat consisting of a soft, insulating undercoat coupled with a hard outer coat. Additionally, the fleece has a significant amount of a thick and brittle kemp.
Sheep raised in the Beskids, both Zackel and Polish Mountain Sheep belong to versatile breeds reared for meat, wool and milk. For years, all sheep products were effectively used and provided existence for highlanders’ families.
Sheep meat, both lamb and mutton, was an important element of the highlanders’ diet, eaten mainly during festive periods and important family events. In other parts of Poland, where pork and beef were more popular, mutton was underestimated. Nevertheless, mountain sheep grazed on natural pastures rich in herbs, unfertilised, with diverse botanical composition of the sward produced meat with less fat, containing biologically active substances and with the preferred profile of fatty acids [34]. Because of its pro-health properties, despite the lack of national tradition in mutton consumption and certain prejudices stemming from the communist past, sheep meat is more and more appreciated. Domestic and foreign consumers show growing, special interest in meat obtained from lambs aged less than 60 days, for which the weight of carcases ranges from 4 to 8 kg. These lambs are fed only with mother’s milk and hay, which results in minimal fat content in the meat. The meat is light pink and has a soft, elastic and pliable texture. It is delicate, succulent, its aroma similar to venison.
The wool is obtained by shearing, which is usually performed twice a year in the summer, outside on pastures and in the winter, before littering, in the fold. For the Zackel sheep, the weight of grease wool ranges from 1.5 to 2 kg. For Polish Mountain Sheep the greasy fleece is heavier and may reach 2.5 kg for one shearing. Wool obtained from both breeds is coarse and not uniform, its properties are much worse in comparison to fine merino wool used for the production of luxury apparel fabrics. Despite poor quality and relatively low price, for many years wool was the most precious sheep product generating the highest income for the sheep owners. During prosperity, it was processed under industrial conditions and used for manufacturing blankets, carpets and other carded products. A significant amount of wool in the form of loose fibres was used as filling of quilts and sleeping bags. Wool was also used by the handicraft sector and often processed using old, traditional methods passed on from one generation to the next. In this way, felt, and cloth used for the production of different elements of traditional highlander dress: pants, hats and gowns were obtained. Additionally, wool was widely used to produce hand spun yarns used to knit traditional highlander sweaters and socks. In the last decades, together with economic transitions and the appearance of competing products made from synthetic fibres the demand for wool products has rapidly decreased and the interest in mountain wool both from the textile industry and the craft sector has dropped heavily. Consequently, mountain wool has drastically lost its economic value and the costs of sheep shearing have outweighed the price of wool. Several attempts to use wool in an unconventional way as fertilisers [35, 36, 37, 38] and soil reinforcement [39] or for production of thermal and acoustic insulating materials [40, 41, 42] and geotextiles [43] have not, so far, gained greater importance. Therefore, wool is now treated as a troublesome waste of sheep breeding, which is buried out on the fields, stored without being scoured or deposed in local, not always legal, landfills.
At present, sheep product which has the highest economic importance is sheep milk. Sheep are milked by hand, twice a day during grazing on the mountain pastures. The milking begins after weaning the lambs and lasts from May until October, around 150 days per year. An ewe produces about 70 litres of milk yearly. It has high nutritional value and contains 15–20% of solids, 4.5–9% of fat, and 4.5–7% of protein. Moreover, the milk is rich in water-soluble vitamins, has a beneficial fatty acid profile and is more calorific than cows’ milk [44, 45, 46, 47]. It is used for the production of traditional local cheeses, the most known are:
Traditional sheep’s cheese made in the Silesian Beskids: (a) bundz; (b) oscypek.
Crushed, grounded and salted mature
All these cheeses are made using traditional methods passed on from generation to generation. First, raw milk is poured into a container, slightly heated and mixed with a rennet. During heating and mixing milk proteins coagulate forming the curd which then is brewed for a few minutes at a temperature of about 70°C. When making
In connection with large differences in altitude above sea level and the related changes in climatic conditions three altitudinal vegetation zone are distinguished in the Silesian Beskids.
The lowest vegetation zone up to 550 m a.s.l. is the foothills. At present, the zone belongs to the most transformed areas of the Silesian Beskids, which is heavily populated and includes a large deforested urban and rural area. There are many buildings, an extensive network of roads and many crop fields, which spread to the edges of the forest on mountain ridges. Only 26% of the zone is covered with forests. They form small patches of deciduous and mixed woods consisting of oak
The lower and upper mountain forest zones at altitudes between 550 and 1250 m a.s.l. are heavily forested. Trees cover up to 60% of the area and currently consist mostly of Norway spruce
The composition of the forest stands changed in the second half of the 19th century as a consequence of high demand for wood. In that time, to increase wood yield the intentional reforestation with the spruce, as the most cost-effective species, was performed. Spruce wood was considered universal and was used in large volumes for the construction of the expanded Carpathian railway network and other industrial applications [48]. Introduction of spruce on a large scale caused the shortage of seeds which had to be imported regardless of their provenance. Due to the lack of local seeds, allochthonous seeds from the surroundings of Innsbruck and Steiermark, other parts of the Austrian Habsburg empire were used [49]. As a result of monocultural reforestation and planting the allochthonous seeds, the forests have become more susceptible to diseases and pest attacks. The consequences have been well visible in recent years, since the beginning of 21th century, as gradual drying-off and decay of trees caused by an outbreak of the spruce bark beetle
Infected forest in Skrzyczne region (the highest peak of the Silesian Beskids).
Apart from the monocultures of spruce stands in the lower forest zone of the Silesian Beskids there are patches of deciduous forests. The most common communities are fertile Carpathian beech wood
Beech forest in the Silesian Beskids.
In addition to extensive wooded areas on mountain ridges, mid-forest clearings of various sizes occur. The clearings are extremely valuable habitats and play an important role as refugia of biodiversity. The most valuable meadow and pasture plant communities include
The valuable plant communities and species on mountain clearings in the Silesian Beskids Mts: (a) priority habitat of
Since the 16th century the clearings had been used as summer pastures, mainly for sheep. In the following two centuries, in the period of quick development of mountain grazing, parts of the forest stands were cut down. As a result, the existing clearings were enlarged and numerous new clearings were established. In the next centuries until the mid-20th century, when the pastoral activity declined and intentional reforestation was performed, the area of mountain clearings slightly decreased. The next rapid decrease of the clearing surface took place at the end of the 20th century, in the period of social and economic transitions connected with the almost complete cessation of sheep grazing. In this time as a result of ecological succession, the clearings began to overgrow with shrubs and trees (Figure 10) [54, 55].
Mountain clearing overgrown with shrubs and trees.
The process of overgrowing clearly shows the great importance of sheep grazing for the maintenance of mountain clearings and protection of their precious plant communities. It is known that sheep eat plants selectively, choosing species with soft leaves and avoiding those with a xeromorphic structure. As a result, sheep inhibit the growth of tall grasses such
It was clear that restoration of pastoral activity in the Silesian Beskids would be a great opportunity to preserve and protect mountain clearings, their precious plant communities and biodiversity as well their unique landscape values.
An example of actions aiming at restoration of pastoralism in the Silesian Beskids is the activity of the Centre of Regional Produce. The centre was founded by Maria and Piotr Kohut and is located in the mountain village of Koniakow, Istebna municipality, in the eastern part of the Silesian Beskids, in the border zone between Poland, Czech Republic and Slovakia. The centre was opened in 2003. Since then, it has been involved in several activities including sheep grazing, production of cheese, sale of sheep products, education and promotion of the pastoral culture. The centre grazes a big, as for Polish conditions, flock which counts over 1000 sheep, the vast majority of it owned by Kohut family. For several years, the size of the flock has been systematically growing from 500 in 2010 to 1000 in 2020. Grazing is carried out traditionally on a nearby, non-forested Ochodzita mountain, at 895 m a.s.l., famous for its beautiful panorama and vast resources of green forage (Figure 11).
Panoramic view from the Ochodzita mountain; (a) to the south; (b) to the north.
The specification of sheep grazing carried out by the centre is presented in Table 1.
Grazing | |
---|---|
Localisation of pastures | Mountains: Ochodzita, Barania Góra, Magurka Radziechowska Villages: Koniaków, Kamesznica |
Ownership of pastures | Private, about 300 land owners |
Shepherds | High qualified with very large professional experience, master exam in the profession of |
Helpers | 8 persons - Hutsul highlanders from Ukraine experienced in sheep grazing |
Shepherd dogs | Tatra Shepherd Dog, Bernese Mountain Dog |
Sheep number | Total: 900–1200 sheep divided into two flocks: 1. milk ewes; 2. ewes with lambs |
Ownership of sheep | 500–600 sheep belong to Kohut family; the remaining to 25 owners |
Sheep breed | Mainly white Polish Mountain Sheep with small number of colour variety; small number of Zackel Sheep and Romanian Tsurkana Sheep |
Pastures | Natural mountain vegetation not fertilised chemically |
Watering | Water drawn from natural sources |
Overnight keep | The sheep spend nights in pastures, in wooden portable enclosures |
Grazing time | From late April/early May until late October |
Milkyield | Daily average yield 0.7 l milk per ewe |
Frequency | Twice a Day |
Number of workers | 6–7 persons |
Milk processing | Milk transported to the Centre for processing |
Products | Different kind of sheep cheeses: |
Time | Winter–spring period: January, February |
Fertility | Reproductive performance: 1–2 per ewe |
Time | Sheep sheared twice a year, in summer and winter |
Greasy fleece weight | 1.5–2 kg of greasy wool |
Specification of grazing carried out by the Centre of Regional Produce in Koniakow.
The sheep milk is used to produce traditional cheeses which are manufactured in a traditional way in the village. During one season from April until September, approximately 50 000 litres of milk are obtained. One portion of the milk is used for the production of 100% sheep cheese. The annual production of this cheese is approximately 5 000 kg, which at the retail price per kilogramme of 10 Euro gives the production value over 50 000 Euro. The second portion of milk is mixed with cow milk and is applied for manufacturing a smoked cow-sheep cheese. The production of mixed cheese reaches yearly 3 500 kg and its value approaches 60 000 Euro. In 2008, the smoked cheese
The centre sells sheep cheese, mutton and other regional products in its shop. The main clients are tourists, local food sellers, as well as restaurant and hotel representatives. The average annual value of goods sold in 2017–2018 amounted to 250 000 Euro of which approximately 70% originated from the sale of sheep’s cheese.
As a member of the National Network of Educational Farms, the Centre offers lectures and workshops which popularise the pastoral culture among the local community and tourists (Figure 12). The educational classes present traditional cheese making methods and wool spinning techniques. The classes are very popular among schoolchildren, students and adults, so the number of participants in the last five years has reached 10 000.
Social activity of the Centre in Koniakow; (a) cheese market; (b) cheese production workshops.
In addition to educational activity, the Centre takes numerous efforts to sustain the pastoral tradition and cultural heritage in the local community and promote them in the Polish society. Each year, the departure of sheep to pastures and their return to the village is connected with several rituals, which became important social events attracting a growing number of tourists (Figure 13). Sheep grazing in the mountain pastures make the mountain landscape more attractive and extensively promote the Silesian Beskids region. Through implementation of the Polish-Slovak interregional project and establishing the Transhumance Pastoralism foundation, the promotional activity of the Centre extended beyond the Polish border. In 2013, to co-memorise Wallachian migration through the Carpathians the foundation organised a sheep hike. As part of the project, Piotr Kohut and 300 sheep travelled 1200 km from Romania through Ukraine to Poland. The hike was accompanied with several social events, which significantly recalled and promoted the pastoral tradition on the international level.
Ceremonial leave of the flock to the summer pasture.
Through its rich activity, the centre provides several work places. In addition to the owners, it employs shepherd helpers for sheep grazing and cheese production, seasonal workers for hay harvesting, sales workers and instructors for educational workshops. The shepherd helpers are usually recruited from Hutsul highlanders from Ukraine, while others come from the local community. The total employment in the high season reaches 15 people.
The beginning of sheep grazing in the Silesian Beskids dates back to the 16th century. The implementation of the pastoral economy transformed the social and economic reality of the mountain villages. The development of the specific pastoral system based on annual migration of flocks between summer highland and winter lowland pastures enabled rational use of natural local resources. Sheep have transformed hitherto inaccessible mountain areas and made human life possible.
For the next two centuries, the pastoral economy was developed, experiencing a relatively long period of prosperity. Sheep were the main source of livelihood and the basis of the economic existence for many highlander families. All sheep products: wool, milk, meat and skin, were greatly appreciated, possessed high economic value and generated reasonable income. Pastoral activity greatly influenced the landscape and contributed to the establishment of new mid-forest clearings with rich plant communities. Shepherding had also impact on the local architecture with wooden huts used by the shepherds in mountain pastures becoming the permanent landscape element. The pastoral economy drove the development of methods for manufacturing sheep’s cheese and processing the wool. For years, the traditional methods were applied and faithfully passed on to the next generations. In connection with the pastoral activity, several customs and rituals were established. All components of the pastoral activity became important elements of the cultural heritage of the region.
From the mid-19th century sheep grazing in the Silesian Beskids was becoming less profitable and the importance of the pastoral economy and its influence on social life of mountain communities was systematically decreasing. At the end of 20th century, after the collapse of the communist system, sheep almost disappeared from the mountains. The restoration of the pastoral economy required decisive action and implementation of several regional and national projects focused on preserving the landscape values, preventing mountain clearings from overgrowing with shrubs and trees, protecting the precious habitats and protecting the cultural heritage of the region.
After several years of implementing these programs, the first positive impacts are visible. The projects became tools to protect, restore and promote sustainable use of the terrestrial mountain ecosystems, stop and reverse land degradation and halt biodiversity loss. The population of sheep grazed on mountain clearings has increased considerably. The existing flocks have been enlarged and some new flocks grazed in earlier abandoned pastures have been formed. Simultaneously, the projects showed that sheep grazing is not just a matter of enthusiasm or a hobby, but rather the natural activity within the region, which can generate economic income. Through various educational and promotional activities of foundations and pastoral centres, the pastoral traditions have been popularised. The activities attract tourists, who are more and more interested in tasty regional sheep products and the cultivation of pastoral tradition.
Based on the results these projects, one can conclude that restoration of the pastoral economy in the Silesian Beskids is not only possible but absolutely necessary. The restoration of pastoral economy fits several European Sustainable Development Goals (SDG) and brings many economic and social benefits. The current activities need to be continued and supported with new initiatives including appropriate legal regulations, subsidies and further projects.
The research leading to these results has received funding from the Norway Grants 2014–2021 via the National Centre for Research and Development.
The authors declare no conflict of interest.
The African oil palm (
The Asian continent concentrates most of the CPO production, led by Indonesia and Malaysia, which together accounts for 85% of the world’s CPO production [4]. However, the limited availability of areas for cultivation in Southeast Asia has opened new frontiers for expansion, culminating in the growth of Latin America’s share in the global production of oil palm [5]. Latin America has the largest suitable area for oil palm cultivation, notably led by Brazil (2,283,000 km2), Peru (458,000 km2), and Colombia (417,000 km2) [5]. Among Latin countries, Colombia is the world’s fourth-largest producer of CPO and the first in the Americas, with an estimated production of 1.67 million tonnes in 2020, followed by Guatemala with 852 thousand tonnes and Honduras with 580 thousand tonnes [4].
Unfortunately, oil palm plantations in this geographical area are affected by a wide variety of pests and diseases that negatively affects productivity and discourage investment in this sector [1]. Notably, “bud-rot type” diseases pose the greatest threat to oil palm plantations in Latin America [6]. Among them,
Fatal Yellowing was first identified by Reiking in 1928 in oil palm plantations in Panama, with cases reported in Colombia, Ecuador, Peru, Costa Rica, Venezuela, Suriname, Nicaragua, and, reportedly, in Central Africa, after that [6]. In Brazil, it was only in 1974 that the first symptomatic individuals were identified and, from the epidemiological explosion that occurred in the 1980s, FY started to represent the greatest threat to oil palm in the country [9]. As a result, several studies began to search for the possible biotic causal agent behind it and its putative vectors [8]. However, the research efforts made for more than 30 years have not exactly pointed out organisms directly linked to FY\'s cause [10, 11, 12, 13, 14, 15, 16]. Some studies also looked for possible abiotic causes, with inconclusive results so far [17, 18, 19]. Recently, techniques such as metabolomics, proteomics, and metagenomics started to be applied to provide insights into the possible FY etiology, initiating a new phase in the process to solve this problem [20, 21, 22].
Although Brazil has more than 30 million hectares with an aptitude for oil palm production, it currently has less than 1% of this area destined for this purpose [23]. Fatal Yellowing is the main contributor to hinder the expansion of the oil palm industry in Brazil, and the attempts to control the emergence of sick plants have not been successful, and its nature remains a mystery [10]. This review intends to analyze descriptively the studies carried out to investigate the FY problem in Brazil, besides pointing out new strategies employed for understanding the development of the disease, confirm the real cause behind it, and develop tools for early diagnostics.
Oil palm is originally from West Africa and adapted to the intertropical areas of Africa, Asia, South and Central America [1]. It is the most profitable oil crop, as it presents a higher yield with a lower production cost [24]. Its oil yield is of the order of 4-6 tonnes per hectare per year of CPO, much higher than that presented by other crops, such as rapeseed (0.69 t), sunflower (0.69 t), and soybeans (0.44 t) [3]. Another positive point is that this crop uses only 6% of the area to produce 36% of the global oil supply, while soy, for example, occupies 40% of the land to generate 26% [4, 24]. Because of that, oil palm stands out as a player fundamental to help the world meet the growing global demand for vegetable oil in 2050 that will be around 240 million tonnes [25, 26].
The expansion of the oil palm industry has been strongly encouraged by governments and private sectors in Southeast Asia [27]. It is by far the most productive region in the world, supplying 85% of the CPO produced, reflecting the rapid expansion of the cultivated area that started in the middle of the last century [25]. The commercial oil palm plantations in Indonesia, for instance, went from 70 thousand hectares in 1961 to 6.78 million hectares in 2018, with a considerable increase of 9.582% during this period [2]. As a result, Southeast Asia production rose to 63.26 million tonnes in 2018, or a 22,378% increase in the period [2, 3].
Africa has not seen an expansion of the oil palm industry as significant as Southeast Asia in the last 60 years [3, 28]. The area occupied by oil palm increased from 3.55 million hectares in 1961 to 4.55 million hectares in 2018 in the African continent, representing an increase of only 33% (Figure 2) [2]. Meanwhile, the Americas now occupy 6% of the international market, producing around 4.89 million tonnes of palm oil in 2018, a 273% increase in the last two decades [2].
The considerable increase in oil palm production was supported mainly by the advances in genetic breeding programs that increased oil productivity more than 2 folds since 1960 [1].
Most of the CPO and its derivatives produced stays in the Asian markets that absorb 51% of the total, led by India, which imports 19.4%, and China 13.0% [29]. The European markets, which import 26%, have the Netherlands (6.1%) and Italy (4.3%) as the leading importers [23]. Africa (12%), the Middle East (4%), and Latin and North America (7%) also have a consumer market for vegetable oils, and palm oil from Southeast Asia helps to supply the demand [29]. The global vegetable oil market allocates 70% of total production to food and 30% to non-food industrial purposes, such as, for example, the production of cosmetics and personal hygiene products (24%) and as a raw material for the production of biofuels (5%) [26].
The increase in global palm oil production in the 21st century is due mainly to new plantations in producing countries, especially in Malaysia and Indonesia [27]. However, due to a reduction in the areas available for expanding cultivation in Southeast Asia, an opportunity opened up to expand to new frontiers to meet the growing global demand for palm oil [5]. As a result, Latin America became one of the most promising regions for oil palm cultivation, as it has one of the largest areas suitable for cultivation, notably represented by Brazil, Peru, and Colombia [5].
Brazil, Colombia, Costa Rica, Ecuador, Guatemala, Honduras, Mexico, Peru, and Venezuela produce together 4.65 million tonnes of palm oil, representing 6% of world production in 2020 [2]. Colombia is the largest oil palm producer in this region and ranks 4th in the World, with 1.61 million tonnes produced in 2018, or 33% from the annual palm oil of Latin America (Figure 1) [2, 30]. Guatemala produced a total of 875 thousand tonnes in 2018 what places the country in the 2nd position in Latin America [2, 31]. Honduras is in the 3rd, followed by Ecuador, Brazil, Costa Rica, and Venezuela [2].
Land use for oil palm cultivation in central and South America since 1980, in hectares. Source: adapted from our wold in data [
The first oil palm plants arrived in Brazil in the 16th century, adapting very well to the Northeast region of the country [32]. The oil palm industry in Brazil stayed as a small industry until 1960, when, due to increasing demand for oil for cooling steel sheets in the national steel park, it started to experience significant growth [33]. In 1967, the oil palm cultivation expanded to the Pará State, in the North region of Brazil, with the first commercial-scale plantations comprising about 3.000 hectares [32].
Driven by technical advances and growth in demand for vegetable oils, there was a significant increase in the cultivated area of oil palm in Brazil, which went from 11 thousand hectares in 1980 to more than 236 thousand hectares in 2008 [3]. Brazil has large areas with the aptitude for oil palm production, favored by climatic conditions similar to the most productive regions in the world [1]. However, until 2014, less than 1% of this area was occupied by commercial plantations [34, 35]. Brazil’s position as the 13th, and 23rd, in palm oil production and on the productivity scale, respectively, in the world, is due mainly to this under-utilization of available areas [3, 32].
Oil palm production is concentrated in Pará state, which accounts for 97.19% of the cultivated area and 97.99% of the national palm oil production, followed by Bahia (1.98%), Roraima, and Amazonas [36]. The expansion of cultivation to already deforested areas in the Amazon and other regions in Brazil is an opportunity to reduce pressure on forests and supply the palm oil demand from the food and energy sectors [35]. To make the plantations more environmentally sustainable, the Brazilian Government launched the agro-ecological zoning (ZAE) program in 2010, a legal mechanism to delimit the oil palm cultivation area [37]. This area include Acre, Amapá, Amazonas, Mato Grosso, Pará, Rondônia, Roraima and Tocantins, part of Maranhão and five municipalities in Goiás state, comprising about 59% of the Brazilian territory [35].
Several fatal syndromes of bud-rot severely affect plantations of oil palm in South and Central America [6, 38]. Bud-rot type disease was reported for the first time on oil palm plantations in Suriname in the 1920s, followed by another incidence in Panama reported by Reinking in 1927 [6]. In general, symptoms of bud-rot type diseases initiate with chlorosis of the youngest leaves and later necrosis that rapidly reach immature tissues of the meristem, causing a collapse of the spear leaf and plant death [9]. Bud rot diseases can take two forms: a lethal form found in Ecuador, Brazil, and in certain zones of Colombia and Suriname, and a non-lethal one, with a high recovery rate, found mainly in the Colombian Llanos [6]. The disease is synonym to a few other names such as “pudrición del cogollo” (PC) in most Spanish speaking countries, “PC típica” (PCt) or “PC diversa” (PCd) in the plantation Palmeras del Ecuador (PDE) in Eastern Ecuador, “amarelecimento fatal” (AF) in Brazil, “spear rot “in Suriname [1, 6, 7].
The first large-scale bud rot damage on oil palm plantations in Latin America was due to the PC disease in northern Colombia, where a field of 2,800 hectares located in the Turbo region was virtually devastated by PC in 1965 [9]. In Suriname, the spear rot was first registered in the Victoria region in 1976 on four-year-old oil palms in a plantation of 1,700 hectares. Despite the phytosanitary practices applied to control the disease, an exponential progression reduced the original area by 85% [39]. In Ecuador, the first PC cases happened in 1976 on four-year-old oil palms on the Pacific slopes of the Ecuadorian cordillera [1], and, like other regions, the plantation was decimated by the disease in a few years [6]. Recently Martinez et al. [7] carried out a study in Colombia to isolate microorganisms and reproduce PC in healthy oil palm plants and, in conclusion, they postulate that the oomycete
Fatal yellowing exhibits, by far, the most dramatic scenario among the bud-rot type diseases of oil palm in the Americas. The factors linked to the emergence of this disease in some countries remain unknown after experiencing more than 50 years of outbreaks in Brazil, Ecuador, Panama, Suriname, Costa Rica, Nicaragua, Honduras, Peru, and Venezuela [6, 9, 10].
The FY disease first appeared in Brazil in 1974, with sporadic occurrences in a field established in 1967 near Benevides, a city in the Pará State [8, 12]. The disease progressed slowly in the following years, from 25 symptomatic plants in 1978 to 125 in 1981. In 1984, ten years after the first report, the number of plants diagnosed with FY was 465 [11]. In the first ten years after its first appearance, the disease progressed in a linear model, and the numbers of affected plants remained more or less the same per unit of time. This mode of progress indicated that the contamination did not occur from plant to plant. However, the numbers of affected plants rose to 9,968 in 1986 and 32,673 in 1987, starting a period of exponential increase [11]. In the first two decades after its first occurrence in Brazil, approximately 100 thousand oil palm trees died from this disease, resulting in the loss of entire plantations [11, 40].
Roguing was then put in place to maintain the source of the inoculum of a possible pathogen to a minimum, eliminating all plants showing symptoms up to one month after the discovery [40, 41]. The oil palm industry promoted training on the fast and precise recognition of FY symptoms to guarantee the effectiveness of this phytosanitary measure [42]. Despite it, the disease kept on occurring in plants between the 15th and the 16th year after planting, making FY one of the main problems of this crop in Brazil. Not surprisingly, this discouraged the expansion of oil palm cultivation in the affected regions [11]. As the inability to identify the causal agent and promote effective control of FY persists, the oil palm industry remains in a state of insecurity to expand in the regions affected by FY [42].
Proper and early disease diagnosis is vital for applying control practices at the right moment. Without an efficient and effective early diagnosis of the disease and the disease-causing agent, any control measures will be inefficient [43]. Until the FY etiology is known and diagnostic systems developed, the only way to find out that a plant has this disease is by checking for characteristic symptoms and signs. Once a plant is diagnosed with FY, it must undergo roguing. In Brazil, symptoms identification in the field is still the only diagnostic system used for FY [8, 12].
An oil palm plant affected by FY shows necrosis or dryness of the spear leaf that evolves towards the base, then the region of the meristem rots, and a foul odor is felt in some cases (Figure 2) [12, 44]. The process of rotting of the meristem region, frequently observed in rainy seasons, motivated the initial designation of the disease as spear leaf rot [8, 40]. After losing the spear leaf, there is a general decline leading to plant death; however, some individuals during this process may temporarily re-issue a new one [12, 18]. In plants affected by FY, chlorosis appears in leaflets at the base of the intermediate leaves, which advances towards the extremity, followed by necroses frequently observed in younger leaves [6]. There is no synchronism between the spear leaf necrosis and the chlorosis of the leaflets. The FY symptoms always begin with leaflets chlorosis, which led to the Fatal Yellowing disease name [1]. In Brazil, the oil palm tree usually dies 7 to 10 months after the onset of the first symptoms, but it can vary depending on the region [41].
Fatal yellowing (FY) disease in oil palm. (a) Oil palm plantation affected by FY; (b) individual showing signs of yellowing and necrosis of the intermediate leaves; (c, d, e) evolution of yellowing and dryness of the spear leaf with the presence of necrotic tissue, and (f) root section of an individual with signs of rot. Source: by authors.
Once the oil palm plant gets affected by FY, the developed bunches can reach the maturation stage and are not affected. However, the immature ones rot, and the inflorescence abort [40, 41]. The root system is visibly affected, and emission of new primary roots reduced, leading to a total cease of roots growth. FY kills the tips of the roots generating new false primary ones. In addition, the root tissue is usually necrotic at the beginning of the appearance of symptoms in the aerial part [45, 46]. On the other hand, no apparent internal symptoms are observed, such as rot or necrosis of the stipe and vascular system, a characteristic that is also seen in PC [41].
The causal agent of FY is still unknown, but a possible genetic solution for this problem exists. This genetic solution resides upon the fact that the American oil palm (
The genus
The American oil palm is endemic to Equatorial America, with natural populations distributed from Central America to northern South America, including the countries of Brazil, Colombia, Costa Rica, Ecuador, French Guiana, Honduras, Mexico, Nicaragua, Panama, Peru, Suriname, and Venezuela [1]. In Surinam, there are dense stands on poor, sandy soil, while in Colombia, it can grow in damp or even swampy situations near or on the banks of rivers [1].
The American oil palm also has a history of use as a source of vegetable oils and other products, but its most important value to the oil palm industry is its capacity to hybridize with the African oil palm [1]. The interest in the germplasm of this species is due to valuable characteristics for breeding programs of the African oil palm, such as slow growth, oil quality (mainly unsaturated oil) [54], and disease resistance, including FY [47].
These two species can sexually cross and generate fertile interspecific hybrids with intermediate characteristics to the two parental species [55]. Some interspecific hybrids between these species are already commercially available, and the Brazilian genetic group of
Independent whether the origin of FY is biotic or abiotic, or a combination of both, once it is finally known, new studies will be necessary to confirm this genetic resistance and gain insights on possible strategies to transfer this resistance to the African oil palm more efficiently and effectively, besides the use of interspecific crosses followed by backcrosses.
After the epidemiological explosion of FY in 1986, Embrapa (the Brazilian Agricultural Research Corporation) started conducting studies on insects as a possible vector of the FY causal agent [8]. As the spread of the disease followed the direction of the prevailing winds, while natural barriers - such as roads, rivers, and glades - were not sufficient to prevent it supported this hypothesis [8, 56]. This hypothesis on a possible entomological role in the spread of FY also resided in the fact that this disease has similarities with the lethal yellowing-type disease that affects other palms. This disease that affects several other palms is due to insect-transmitted phytoplasmas [57]. Initially, from inventory obtained in plantations affected by FY in the municipalities of Alvaraes, in the Amazonas State, and Benevides, in the Pará State, the main insects suspected of being responsible for the transmission corresponded to
Initially, an inventory of insects captured directly on the oil palm plantations located inside and outside areas with FY occurrence was generated. Healthy oil palm plants, isolated in cages made of wood and nylon canvas, received populations of the inventoried insects, and the plants monitored for symptoms appearance. After using almost one million insects in the FY transmission test, no symptomatic plant appeared, and there was no relationship between the affected areas with the collected insect fauna [15, 58]. Additional studies have attempted to establish a link between the insects
Another study attempted to investigate the relationship between the presence of homopterans in the vegetation cover in oil palm plantations and the occurrence of FY [12]. No relation between the vegetation cover and FY occurrence appeared as the disease manifested itself either in an area covered with
Phytoplasmas are prokaryotes of the Class Mollicutes that cause diseases in several plant species, including several economically important ones [59]. As biotrophic parasites, they colonize the elements riddled with the phloem and can also be found inside the vectors [60]. These organisms are responsible for Lethal Yellowing (LF), a fatal disease that affects the coconut (
Insects from the Homoptera order, popularly known as leafhoppers, are the vectors for most phytoplasmas causing disease in plants [63]. Biological characteristics, symptoms, and specificity of the insect vector were the focus of the first studies aiming to associate phytoplasmas with plant diseases [64, 65]. Later, new and more accurate DNA-based techniques started to dominate these studies, leading to the production of specific oligonucleotides for diagnosis [65].
Transmission electron microscopy was, for many years, the tool used for the detection and study of the cytological interaction between phytoplasmas and the hosts [66]. Studies using this tool were not successful in associating phytoplasma with FY, been replaced by new molecular techniques for the same purpose [8]. Studies carried out by Brioso et al. [67, 68] using nested-PCR in oil palm plants symptomatic for FY found just a very few samples positives for the presence of phytoplasmas from the SrI and 16SrI groups, which do not allow to associate these phytoplasmas to FY. An attempt to reproduce the disease was carried out by grafting intermediate leaf tips with FY into healthy seedling petioles and, during the period of two years, healthy individuals did not show symptoms characteristic of FY and, thus, the hypothesis proposing phytoplasma as the causal agent was discarded [12].
In the attempt to establish a causal relationship between plant pathogenic fungi, bacteria, and nematodes with FY, some studies tried to reproduce the symptoms in healthy young and adult oil palm plants inoculated with some of these microorganisms previously isolated from symptomatic plants [69, 70].
A pathogenicity test focused on studying the growth, reproductive and developmental habits of microorganisms, included one-year-old nursery plants with individual inoculations and a mixture of three fungi (
Interestingly, some authors have observed similarities between the disease PC in Colombia and FY in Brazil. Furthermore, the oomycete
Nematodes are typically wormlike invertebrates able to live in the soil or inside plant structures such as roots, stems, leaves, and flowers and can cause morphological and developmental changes in their hosts [71]. The hypothesis of a nematode as a causative agent of FY came from observations of FY and the red ring disease - caused by the nematode
Other plant pathogens studied as potential causal agents of FY in oil palm were viruses and viroids. Several methods, including mechanical transmission, grafting, pollen-mediated dispersion, transmission electron microscopy, nested RT-PCR, RCA - rolling circle amplification, and electrophoresis, were used to test the hypothesis of a virus or a viroid as the causal agent of FY, without success [8, 10].
Lin et al. [73] evaluated extracts from plants with and without FY using the polyacrylamide gel electrophoresis technique, and the band patterns generated in both samples did not reveal any apparent difference. The same author also carried out a study to purify virus particles via separation with a fractional density gradient with no success [74]. Kitajima [75] evaluated ultrafine tissues from roots, leaves, and spear leaf of symptomatic and asymptomatic individuals by transmission electron microscopy, but no pathogen could be associated with FY.
Other studies have directed their efforts towards viroids, which are the smallest known phytopathogens, consisting basically of a single-stranded, circular RNA molecule not encapsulated [76, 77]. Beuther et al. [13] searched for viroids and viroid-like RNAs in oil palm plants using two-dimensional gel electrophoresis and return gel electrophoresis of nucleic acid extracts, with no success in showing a link between this type of pathogen and FY.
The initial pieces of evidence of a possible abiotic cause for FY came from observations made about the indefinite dissemination pattern in affected areas, with an exponential growth form not observed in the case of biotic stresses [78, 79]. Among the possible abiotic causes linked to the appearance of FY, there are lower and higher amounts of water, high or low temperature, high content of soluble salts in the soil, soil pH unsuitable for oil palm, nutritional deficiencies or excesses, presence of toxic organic compounds and intensity and balance of nutrients [78].
The regions with oil palm plantations and FY occurrence located in the North region of Brazil have soils with patches of quartz sand interspersed with patches of lateritic concretions and are subject to prolonged floodings, 5 to 6 months per year [41]. Thus, studies started aiming to understand the composition of the soil and its influence on FY emergence.
The concentrations of Cu, Fe, Mn, and Zn in the leaves of healthy and symptomatic oil palm plants and resistant interspecific hybrids were determined and found out that their concentrations were below the ideal range, suggesting their involvement in the appearance of FY [80]. Compact soils that stay temporarily saturated by rainfall suffer oxidation by anoxia, making it impossible for plants to absorb Fe [80]. Based on these observations, applications of ferrous sulfate were carried out on plants under different stages of FY, but after 120 days of the experiment, there was no regression of the disease in the evaluated oil palms [80].
The physical properties of the soil from areas with the occurrence of FY revealed that they were naturally well-drained and deep but had a thickening or compacting between the depths of 30 cm and 60 cm, as well as the occurrence of speckles in this depth, which results in soil saturation in the superficial layer during the rainfall season [81]. Bernardes [82] carried out chemical analysis on roots of symptomatic plants, and the results did not allow to pinpoint any element imbalance that could be responsible for FY. Another fact that needs consideration as possibly linked to a potential cause for the disease is the fact that at the moment when the first symptoms appear in the aerial part, the root system is severely impaired, which explains the plants’ lack of response to fertilization and other interventions [82].
A series of field observations made in the heart of the oil palm production area in Brazil led to new hypotheses for a possible abiotic cause for FY [83]. The main field observations taken into consideration were: a higher occurrence of flooding in oil palm plantations, in comparison to the previous level, observed under native vegetation cover; the layers close to the soil surface without vegetation cover or with oil palm tend to stay close to water saturation for periods much longer than in the native forest; the presence of mottled-iron reduction in the profile of the oil palm plantations, and the redox-potential values (Eh) below −200 mV; and the presence of reduced iron ions on the soil surface in oil palm plantations during periods of intense rain [83].
The new hypotheses were brought together and summarized as: Deficient aeration reduces the potential for oxy-reduction in the soil, causing changes in the ionic composition of the soil solution (reduction of Fe3+ ions; NO3+; Mn3+). The soil solution with a high concentration of reduced ions initially causes damage to the root system (Figure 3) predisposing the oil palm plant to physiological disturbances (passive poisoning and attacks of secondary pathogens) whose symptoms are known as FY [84].
Oil palm plant showing reduction of the root system in hypoxia conditions (A), and soil clouds showing the typical reductimorphic or oximorphic color mottles caused by stagnating soil environment (B). Source: Wenceslau Teixeira.
To gain insights into the idea of oxygen deficiency (hypoxia) in the origin of FY, a study by Encinas [85] evaluate the influence of land use and temporal variations on the dynamics of nutrients in the solution of soil and water at an oil palm plantation and a nearby area still with primary forest. Another by Muniz [83] compared the changes in water flow at an oil palm plantation and a nearby area still with native vegetation cover and evaluated its effects on iron dynamics and the structure of the soil. These two studies gathered additional shreds of evidence to further support this hypothesis, such as the electrical conductivity increased during a long flooding period (95 days), indicating that ions from the aggregates migrate to the solution; the soil pH increases after the initial flooding period, reaching values close to neutrality, with a subsequent reduction, but above the values found in aerated soil; the soil redox potential decreases during the flooding period, forming a highly reducing environment; the total carbon contained in the macroaggregates reduced after flooding for a period of 11 days; the iron contained in the aggregates of Yellow Latosols with medium texture migrates to the soil solution under flooding conditions; there is a high negative correlation between the iron in the flooding solution and the DMG of the aggregates in the Yellow Latosols, and flooding for a period of 11 days promotes the destabilization of aggregates of Yellow Latosols with medium goethite texture.
The so-called ‘omics’ techniques (Figure 4) provide new opportunities to study oil palm FY. To get insights on FY possible causal agent, different research groups in Brazil have used metagenomics, metabolomics, and proteomics analysis [20, 21, 22]. To our knowledge, no work focusing on transcriptomics and FY has been published yet. The most commonly used approach in these studies is to compare healthy plants (without symptoms of FY) to those showing disease symptoms at different stages of progression. In contrast to more traditional non-molecular studies of FY, these techniques provide a global glimpse of the organism by looking at the associated microbiota (metagenomics), the complete protein content (proteomics), or metabolite content (metabolomics) of cells.
Schematic showing a healthy oil palm tree (green leaves) and another one (yellow leaves) showing fatal yellowing (FY) symptoms. Different molecular techniques such as metagenomics, metabolomics and proteomics can be used to compare these contrasting biological situations. Metagenomics is a culture-independent technique that can be used to identify the microorganisms present. Metabolomics can used to identify and quantify cellular metabolites. Proteomics allows the identification of differentially expressed proteins. These ‘omics’ techniques are important high throughput tools that have been used to understand the biology of oil palm when challenged by FY disease. (credit: Clarissa Kruger).
Koch’s postulate was fundamental to the identification of disease-causing microorganisms [86]. In short, the strategy of isolating and cultivating the potential pathogen, and inoculating it into a healthy organism to confirm the symptoms of the disease, brought many advances to the study of infectious diseases [87]. More recently, due mainly to the advent of next-generation sequencing (NGS) technologies, the frontiers of microbiology expanded to those microorganisms that we cannot cultivate by classical microbiology techniques. That has opened the possibility to test the hypothesis that a microorganism not grown in vitro easily is the cause of FY [88]. If this is the case, metagenomics would be the technique to study FY.
Metagenomics is a culture-independent approach to study microbial communities. A metagenomics strategy allows one to skip the step of isolation and cultivation of microbial species. Metagenomics studies can contribute to elucidate the identity and/or the genetic and metabolic capabilities of the microorganisms present in a sample, including any that are potentially pathogenic [89].
In this sense, metagenomics complements the classic techniques of isolation and cultivation of microorganisms, and one can apply it to study different classes of microorganisms (e.g., viruses, bacteria, fungi, archaea) [22, 90, 91, 92]. Metagenomics protocols begin with the extraction of total DNA from the sample of interest, which contains microorganisms. Samples can be many different ones, such as soil or plant parts with FY disease symptoms. There are distinct ways to study the microbial community from this DNA. Many studies in different plants use the ribosomal RNA (rRNA) gene or ITS amplification approach (i.e., PCR amplification with specific primers) to identify the microorganisms present, including a potential pathogen [93, 94, 95].
16S rRNA gene-specific primers amplify bacterial and archaeal sequences (16S rDNA). Similarly, the 18S rRNA gene and the ITS-specific primers amplify fungal sequences. The ITS refers to the internal transcribed spacer, the DNA situated between the small-subunit ribosomal RNA and large-subunit rRNA genes. The 16S rDNA, 18S rDNA, and the ITS regions are highly polymorphic, thus allowing taxonomical identification of the microorganisms present in a sample. The PCR-amplified DNA is then sequenced and submitted to bioinformatics analysis to compare the obtained sequences with sequence databanks, leading to a putative microorganism. In summary, this metagenomics approach that combines PCR amplification with NGS allows identifying microorganisms present in the community [96].
The first metagenomics work to use ITS amplification and high throughput NGS to study FY in Brazil was performed by Costa et al. [22], who evaluated fungal communities associated with leaves of oil palm plants, with and without symptoms of FY. Leaves from health plants and from plants showing FY symptoms in three different disease stages (stages 2, 5, and 8) were obtained. Because of the similarities between PC and FY, using primers specific to the genus
The Costa et al. [22] study reported the analyses of fungal diversity using the ITS region. Results showed that the fungal community in different healthy asymptomatic oil palm leaves are more similar to each other than those presenting FY disease symptoms. The fungal communities were not the same among all the symptomatic samples, and were not consistent even between samples at the same stage of FY disease. Importantly, no fungal taxon had its relative proportion increased in leaves across all the FY diseased oil palm plants. It was hypothesized that the changes observed in the fungal community composition could be a secondary effect of FY disease. Similar metagenomic studies to analyze the viral, bacterial and archaeal communities associated with FY are needed.
A less common metagenomic approach that can also be used to study plant disease is to assemble genomes from the metagenome obtained from plants showing symptoms of disease. In this case, instead of using PCR to amplify a specific gene, one can completely sequence the DNA extracted from the samples of interest, and use bioinformatics tools to assemble genomes (metagenome-assembled genomes) of the microorganisms present. This type of methodology allows, in addition to identifying microorganisms present, access to their genomes. This creates the possibility of studying the genetic relationship among the species present, and predicting metabolic capabilities as well as the interactions between the organisms of the community [97]. One limitation to this method, however, is that the plant host genome sequence needs to be available and subtracted
Proteome designates the set of proteins expressed by a cell, tissue, or organism at any given time [99]. Proteomic tools make it possible to obtain a protein profile with precision and sensitivity with the aid of electrophoresis, chromatography, mass spectrometry, and bioinformatics [99]. Proteomics is more and more used nowadays to understand plant responses to different biotic and abiotic stress conditions [100, 101].
In this context, and based on the hypothesis that the primary stress behind FY was abiotic and present in the soil, proteomics was applied to study this disease [21]. This hypothesis is based on observations regarding symptoms seen in the root system before they appeared in the aerial part [83]. Soil compaction, which hinders drainage and subject the roots to long periods of flooding in a hypoxia condition, would be in the origin of the stress [83].
Nascimento et al. [21] carried out a proteomic analysis to compare the protein profiles from symptomatic and asymptomatic oil palm plants, employing the mass spectrometry technique. The study looked for proteins linked to tolerance induction to relate the different areas collected and the distinct stages of the disease, analyzing the roots of symptomatic plants in early, intermediate, and final stages.
Proteins involved in the metabolism of phenylpropanoids and lignins, with a recognized role in reducing the effects of biotic and abiotic stress, were negatively regulated in symptomatic individuals, aggravating FY symptoms. In asymptomatic plants, enzymes such as S-adenosylmethionine - with a crucial role in methionine’s biosynthetic metabolism - showed a recognized action in response to the stress. Plants with FY symptoms showed some pathogen-related proteins positively regulated, implying a progression of infection by biotic agents [21].
The hypothesis of a possible physiological dysfunction caused by factors present in the soil was reinforced by the large accumulation of antioxidant proteins in asymptomatic individuals [21]. The participation of the antioxidant system may indicate some level of resistance, considering that this system is vital for plants in conditions of soil flooding [102]. In addition, the accumulation of aldehyde dehydrogenase may indicate that the root system is under an anaerobic condition as it converts the acetaldehyde, promoting plant survival in this condition [21, 103]. Thus, these results indicate that plants affected by FY are in abiotic stress conditions and, with the damages done to the roots, it becomes a gateway for several opportunistic organisms [21].
In contrast to proteomics, metabolomics refers to a comprehensive analysis to identify the set of metabolites present in a sample with the aid of analytical techniques, such as liquid chromatographies or liquid–gas, associated or not with mass spectrometry, among others [104].
Rodrigues-Neto et al. [20] performed the first metabolomics work to study FY in Brazil using an untargeted metabolomics strategy to prospect metabolites differentially expressed in the leaves of FY symptomatic and asymptomatic plants. A high throughput method based on metabolic fingerprinting MS, using UHPLC coupled to high-resolution mass spectrometry (HRMS), was employed, and chemometric analysis, PCA and PLS-DA, were used to evaluate metabolic differences. This study aimed at prospecting a biomarker for FY early diagnosis, besides gaining insights on pathways responsive to this disease valuable for future improvement studies.
Nine secondary metabolites were detected in a higher concentration in the healthy plants in comparison to the FY affected ones: Glycerophosphorylcholine, arginine, asparagine, paniculatin or apigenin 6,8-di-C-hexose, tyramine, Chlorophyllide, 1,2-dihexanoyl-sn-glycero-3-phosphoethanolamine, proline, malvidin 3-glucoside-5-(6″-malonylglucoside) or kaempferol 7-methyl ether 3-[3-hydroxy-3-methylglutaryl-(1–> 6)]-[apiosyl-(1–> 2)-galactoside]. These metabolites made possible to identify different metabolic pathways that have been affected by the FY, such as the glycerophospholipid metabolism, the isoquinoline alkaloid biosynthesis, the flavonoid biosynthesis, the tetrapyrrole biosynthesis and citrate cycle derivatives pathways.
Unfortunately, due to the fact that these metabolites are already described in the literature as linked to other types of stress, they are not good candidate for biomarkers; except for two of them, glycerophosphorylcholine and 1,2-dihexanoyl-sn-glycero-3-phosphoethanolamine [20].
Fatal yellowing disease represents a threat of great magnitude to the Brazilian oil palm industry. For decades, several studies attempted to identify its causal agent without success. As a result, no measures used today can effectively reduce the economic loss for the oil palm industry due to this disease. The only glimpse of hope in solving this problem still resides in the genetic resistance found in the American oil palm. However, the road to transfer this resistance through interspecific crosses and backcrosses is very long and has many uncertainties.
The search for the primary stress leading to FY must go on, whether it is of biotic or abiotic origin - or the combination of both. Only then might be able to block its occurrence, or, if not possible to do that, develop early diagnostic tools to reduce its spread to a minimum.
Recent studies using single omics analysis have shown that these new technics can take the etiological studies regarding FY in oil palm to another level. We postulate that transcriptomics should be the next step in using omics to gain further insights regarding this disease. Even more, we believe that it should be done under the scope of a multi-omics integration (MOI) strategy, together with metabolomics, proteomics, and ionomics, at least.
The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: The grant (01.13.0315.00 - DendêPalm Project) for this study was awarded by the Brazilian Innovation Agency - FINEP.
The authors declare no conflict of interest.
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On September, 29th 2006 he has won a post PhD fellowship from the university of Bologna (from October 2006 to October 2008), at the competitive examination he was ranked first in the industrial engineering area. He extensively served as referee for several international journals. He is author/coauthor of more than 100 research papers. He has been involved in some projects supported by MURST and European Community. His research interests include pattern recognition, bioinformatics, and biometric systems (fingerprint classification and recognition, signature verification, face recognition).",institutionString:null,institution:null},{id:"496",title:"Dr.",name:"Carlos",middleName:null,surname:"Leon",slug:"carlos-leon",fullName:"Carlos Leon",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"University of Seville",country:{name:"Spain"}}},{id:"512",title:"Dr.",name:"Dayang",middleName:null,surname:"Jawawi",slug:"dayang-jawawi",fullName:"Dayang Jawawi",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"University of Technology Malaysia",country:{name:"Malaysia"}}},{id:"528",title:"Dr.",name:"Kresimir",middleName:null,surname:"Delac",slug:"kresimir-delac",fullName:"Kresimir Delac",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/528/images/system/528.jpg",biography:"K. 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From 1985 to 1986, he was a Research Fellow in the Research Institute for Electronic Equipment, ZZU AD, Plovdiv, Bulgaria. In 1986, he joined the Department of Control Systems, Technical University of Sofia at the Plovdiv campus, where he is presently a Full Professor. He has held long-term visiting Professor/Scholar positions at various institutions in South Korea, Turkey, Mexico, Greece, Belgium, UK, and Germany. And he has coauthored one book and authored or coauthored more than 80 research papers in conference proceedings and journals. 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His research interests include the application of agent technology for achieving agile control in the manufacturing environment.",institutionString:null,institution:null},{id:"605",title:"Prof",name:"Dil",middleName:null,surname:"Hussain",slug:"dil-hussain",fullName:"Dil Hussain",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/605/images/system/605.jpg",biography:"Dr. Dil Muhammad Akbar Hussain is a professor of Electronics Engineering & Computer Science at the Department of Energy Technology, Aalborg University Denmark. Professor Akbar has a Master degree in Digital Electronics from Govt. College University, Lahore Pakistan and a P-hD degree in Control Engineering from the School of Engineering and Applied Sciences, University of Sussex United Kingdom. Aalborg University has Two Satellite Campuses, one in Copenhagen (Aalborg University Copenhagen) and the other in Esbjerg (Aalborg University Esbjerg).\n· He is a member of prestigious IEEE (Institute of Electrical and Electronics Engineers), and IAENG (International Association of Engineers) organizations. \n· He is the chief Editor of the Journal of Software Engineering.\n· He is the member of the Editorial Board of International Journal of Computer Science and Software Technology (IJCSST) and International Journal of Computer Engineering and Information Technology. \n· He is also the Editor of Communication in Computer and Information Science CCIS-20 by Springer.\n· Reviewer For Many Conferences\nHe is the lead person in making collaboration agreements between Aalborg University and many universities of Pakistan, for which the MOU’s (Memorandum of Understanding) have been signed.\nProfessor Akbar is working in Academia since 1990, he started his career as a Lab demonstrator/TA at the University of Sussex. After finishing his P. hD degree in 1992, he served in the Industry as a Scientific Officer and continued his academic career as a visiting scholar for a number of educational institutions. In 1996 he joined National University of Science & Technology Pakistan (NUST) as an Associate Professor; NUST is one of the top few universities in Pakistan. In 1999 he joined an International Company Lineo Inc, Canada as Manager Compiler Group, where he headed the group for developing Compiler Tool Chain and Porting of Operating Systems for the BLACKfin processor. The processor development was a joint venture by Intel and Analog Devices. In 2002 Lineo Inc., was taken over by another company, so he joined Aalborg University Denmark as an Assistant Professor.\nProfessor Akbar has truly a multi-disciplined career and he continued his legacy and making progress in many areas of his interests both in teaching and research. He has contributed in stochastic estimation of control area especially, in the Multiple Target Tracking and Interactive Multiple Model (IMM) research, Ball & Beam Control Problem, Robotics, Levitation Control. He has contributed in developing Algorithms for Fingerprint Matching, Computer Vision and Face Recognition. He has been supervising Pattern Recognition, Formal Languages and Distributed Processing projects for several years. He has reviewed many books on Management, Computer Science. Currently, he is an active and permanent reviewer for many international conferences and symposia and the program committee member for many international conferences.\nIn teaching he has taught the core computer science subjects like, Digital Design, Real Time Embedded System Programming, Operating Systems, Software Engineering, Data Structures, Databases, Compiler Construction. 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He is an author and co-author of scientific publications covering analysis and processing of biomedical images and development of database systems.",institutionString:"University of Silesia",institution:null}]},{type:"book",id:"6843",title:"Biomechanics",subtitle:null,coverURL:"https://cdn.intechopen.com/books/images_new/6843.jpg",slug:"biomechanics",publishedDate:"January 30th 2019",editedByType:"Edited by",bookSignature:"Hadi Mohammadi",hash:"85132976010be1d7f3dbd88662b785e5",volumeInSeries:4,fullTitle:"Biomechanics",editors:[{id:"212432",title:"Prof.",name:"Hadi",middleName:null,surname:"Mohammadi",slug:"hadi-mohammadi",fullName:"Hadi Mohammadi",profilePictureURL:"https://mts.intechopen.com/storage/users/212432/images/system/212432.jpeg",biography:"Dr. Hadi Mohammadi is a biomedical engineer with hands-on experience in the design and development of many engineering structures and medical devices through various projects that he has been involved in over the past twenty years. Dr. Mohammadi received his BSc. and MSc. degrees in Mechanical Engineering from Sharif University of Technology, Tehran, Iran, and his PhD. degree in Biomedical Engineering (biomaterials) from the University of Western Ontario. He was a postdoctoral trainee for almost four years at University of Calgary and Harvard Medical School. He is an industry innovator having created the technology to produce lifelike synthetic platforms that can be used for the simulation of almost all cardiovascular reconstructive surgeries. He’s been heavily involved in the design and development of cardiovascular devices and technology for the past 10 years. 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Radiotherapy and Nuclear Medicine Technology has always been my aspiration and my life. As years passed I accumulated a tremendous amount of skills and knowledge in Radiotherapy and Nuclear Medicine, Conventional Radiology, Radiation Protection, Bioinformatics Technology, PACS, Image processing, clinically and lecturing that will enable me to provide a valuable service to the community as a Researcher and Consultant in this field. My method of translating this into day to day in clinical practice is non-exhaustible and my habit of exchanging knowledge and expertise with others in those fields is the code and secret of success.",institutionString:null,institution:{name:"Majmaah University",country:{name:"Saudi Arabia"}}},{id:"313277",title:"Dr.",name:"Bartłomiej",middleName:null,surname:"Płaczek",slug:"bartlomiej-placzek",fullName:"Bartłomiej Płaczek",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/313277/images/system/313277.jpg",biography:"Bartłomiej Płaczek, MSc (2002), Ph.D. (2005), Habilitation (2016), is a professor at the University of Silesia, Institute of Computer Science, Poland, and an expert from the National Centre for Research and Development. His research interests include sensor networks, smart sensors, intelligent systems, and image processing with applications in healthcare and medicine. He is the author or co-author of more than seventy papers in peer-reviewed journals and conferences as well as the co-author of several books. He serves as a reviewer for many scientific journals, international conferences, and research foundations. Since 2010, Dr. Placzek has been a reviewer of grants and projects (including EU projects) in the field of information technologies.",institutionString:"University of Silesia",institution:{name:"University of Silesia",country:{name:"Poland"}}},{id:"35000",title:"Prof.",name:"Ulrich H.P",middleName:"H.P.",surname:"Fischer",slug:"ulrich-h.p-fischer",fullName:"Ulrich H.P Fischer",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/35000/images/3052_n.jpg",biography:"Academic and Professional Background\nUlrich H. P. has Diploma and PhD degrees in Physics from the Free University Berlin, Germany. He has been working on research positions in the Heinrich-Hertz-Institute in Germany. Several international research projects has been performed with European partners from France, Netherlands, Norway and the UK. He is currently Professor of Communications Systems at the Harz University of Applied Sciences, Germany.\n\nPublications and Publishing\nHe has edited one book, a special interest book about ‘Optoelectronic Packaging’ (VDE, Berlin, Germany), and has published over 100 papers and is owner of several international patents for WDM over POF key elements.\n\nKey Research and Consulting Interests\nUlrich’s research activity has always been related to Spectroscopy and Optical Communications Technology. Specific current interests include the validation of complex instruments, and the application of VR technology to the development and testing of measurement systems. He has been reviewer for several publications of the Optical Society of America\\'s including Photonics Technology Letters and Applied Optics.\n\nPersonal Interests\nThese include motor cycling in a very relaxed manner and performing martial arts.",institutionString:null,institution:{name:"Charité",country:{name:"Germany"}}},{id:"341622",title:"Ph.D.",name:"Eduardo",middleName:null,surname:"Rojas Alvarez",slug:"eduardo-rojas-alvarez",fullName:"Eduardo Rojas Alvarez",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/341622/images/15892_n.jpg",biography:null,institutionString:null,institution:{name:"University of Cuenca",country:{name:"Ecuador"}}},{id:"215610",title:"Prof.",name:"Muhammad",middleName:null,surname:"Sarfraz",slug:"muhammad-sarfraz",fullName:"Muhammad Sarfraz",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/215610/images/system/215610.jpeg",biography:"Muhammad Sarfraz is a professor in the Department of Information Science, Kuwait University, Kuwait. His research interests include optimization, computer graphics, computer vision, image processing, machine learning, pattern recognition, soft computing, data science, and intelligent systems. Prof. Sarfraz has been a keynote/invited speaker at various platforms around the globe. He has advised/supervised more than 110 students for their MSc and Ph.D. theses. He has published more than 400 publications as books, journal articles, and conference papers. He has authored and/or edited around seventy books. Prof. Sarfraz is a member of various professional societies. He is a chair and member of international advisory committees and organizing committees of numerous international conferences. He is also an editor and editor in chief for various international journals.",institutionString:"Kuwait University",institution:{name:"Kuwait University",country:{name:"Kuwait"}}},{id:"32650",title:"Prof.",name:"Lukas",middleName:"Willem",surname:"Snyman",slug:"lukas-snyman",fullName:"Lukas Snyman",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/32650/images/4136_n.jpg",biography:"Lukas Willem Snyman received his basic education at primary and high schools in South Africa, Eastern Cape. He enrolled at today's Nelson Metropolitan University and graduated from this university with a BSc in Physics and Mathematics, B.Sc Honors in Physics, MSc in Semiconductor Physics, and a Ph.D. in Semiconductor Physics in 1987. After his studies, he chose an academic career and devoted his energy to the teaching of physics to first, second, and third-year students. After positions as a lecturer at the University of Port Elizabeth, he accepted a position as Associate Professor at the University of Pretoria, South Africa.\r\n\r\nIn 1992, he motivates the concept of 'television and computer-based education” as means to reach large student numbers with only the best of teaching expertise and publishes an article on the concept in the SA Journal of Higher Education of 1993 (and later in 2003). The University of Pretoria subsequently approved a series of test projects on the concept with outreach to Mamelodi and Eerste Rust in 1993. In 1994, the University established a 'Unit for Telematic Education ' as a support section for multiple faculties at the University of Pretoria. In subsequent years, the concept of 'telematic education” subsequently becomes well established in academic circles in South Africa, grew in popularity, and is adopted by many universities and colleges throughout South Africa as a medium of enhancing education and training, as a method to reaching out to far out communities, and as a means to enhance study from the home environment.\r\n\r\nProfessor Snyman in subsequent years pursued research in semiconductor physics, semiconductor devices, microelectronics, and optoelectronics.\r\n\r\nIn 2000 he joined the TUT as a full professor. Here served for a period as head of the Department of Electronic Engineering. Here he makes contributions to solar energy development, microwave and optoelectronic device development, silicon photonics, as well as contributions to new mobile telecommunication systems and network planning in SA.\r\n\r\nCurrently, he teaches electronics and telecommunications at the TUT to audiences ranging from first-year students to Ph.D. level.\r\n\r\nFor his research in the field of 'Silicon Photonics” since 1990, he has published (as author and co-author) about thirty internationally reviewed articles in scientific journals, contributed to more than forty international conferences, about 25 South African provisional patents (as inventor and co-inventor), 8 PCT international patent applications until now. Of these, two USA patents applications, two European Patents, two Korean patents, and ten SA patents have been granted. A further 4 USA patents, 5 European patents, 3 Korean patents, 3 Chinese patents, and 3 Japanese patents are currently under consideration.\r\n\r\nRecently he has also published an extensive scholarly chapter in an internet open access book on 'Integrating Microphotonic Systems and MOEMS into standard Silicon CMOS Integrated circuitry”.\r\n\r\nFurthermore, Professor Snyman recently steered a new initiative at the TUT by introducing a 'Laboratory for Innovative Electronic Systems ' at the Department of Electrical Engineering. The model of this laboratory or center is to primarily combine outputs as achieved by high-level research with lower-level system development and entrepreneurship in a technical university environment. Students are allocated to projects at different levels with PhDs and Master students allocated to the generation of new knowledge and new technologies, while students at the diploma and Baccalaureus level are allocated to electronic systems development with a direct and a near application for application in industry or the commercial and public sectors in South Africa.\r\n\r\nProfessor Snyman received the WIRSAM Award of 1983 and the WIRSAM Award in 1985 in South Africa for best research papers by a young scientist at two international conferences on electron microscopy in South Africa. He subsequently received the SA Microelectronics Award for the best dissertation emanating from studies executed at a South African university in the field of Physics and Microelectronics in South Africa in 1987. In October of 2011, Professor Snyman received the prestigious Institutional Award for 'Innovator of the Year” for 2010 at the Tshwane University of Technology, South Africa. This award was based on the number of patents recognized and granted by local and international institutions as well as for his contributions concerning innovation at the TUT.",institutionString:null,institution:{name:"University of South Africa",country:{name:"South Africa"}}},{id:"317279",title:"Mr.",name:"Ali",middleName:"Usama",surname:"Syed",slug:"ali-syed",fullName:"Ali Syed",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/317279/images/16024_n.png",biography:"A creative, talented, and innovative young professional who is dedicated, well organized, and capable research fellow with two years of experience in graduate-level research, published in engineering journals and book, with related expertise in Bio-robotics, equally passionate about the aesthetics of the mechanical and electronic system, obtained expertise in the use of MS Office, MATLAB, SolidWorks, LabVIEW, Proteus, Fusion 360, having a grasp on python, C++ and assembly language, possess proven ability in acquiring research grants, previous appointments with social and educational societies with experience in administration, current affiliations with IEEE and Web of Science, a confident presenter at conferences and teacher in classrooms, able to explain complex information to audiences of all levels.",institutionString:null,institution:{name:"Air University",country:{name:"Pakistan"}}},{id:"75526",title:"Ph.D.",name:"Zihni Onur",middleName:null,surname:"Uygun",slug:"zihni-onur-uygun",fullName:"Zihni Onur Uygun",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/75526/images/12_n.jpg",biography:"My undergraduate education and my Master of Science educations at Ege University and at Çanakkale Onsekiz Mart University have given me a firm foundation in Biochemistry, Analytical Chemistry, Biosensors, Bioelectronics, Physical Chemistry and Medicine. After obtaining my degree as a MSc in analytical chemistry, I started working as a research assistant in Ege University Medical Faculty in 2014. In parallel, I enrolled to the MSc program at the Department of Medical Biochemistry at Ege University to gain deeper knowledge on medical and biochemical sciences as well as clinical chemistry in 2014. In my PhD I deeply researched on biosensors and bioelectronics and finished in 2020. Now I have eleven SCI-Expanded Index published papers, 6 international book chapters, referee assignments for different SCIE journals, one international patent pending, several international awards, projects and bursaries. In parallel to my research assistant position at Ege University Medical Faculty, Department of Medical Biochemistry, in April 2016, I also founded a Start-Up Company (Denosens Biotechnology LTD) by the support of The Scientific and Technological Research Council of Turkey. Currently, I am also working as a CEO in Denosens Biotechnology. The main purposes of the company, which carries out R&D as a research center, are to develop new generation biosensors and sensors for both point-of-care diagnostics; such as glucose, lactate, cholesterol and cancer biomarker detections. My specific experimental and instrumental skills are Biochemistry, Biosensor, Analytical Chemistry, Electrochemistry, Mobile phone based point-of-care diagnostic device, POCTs and Patient interface designs, HPLC, Tandem Mass Spectrometry, Spectrophotometry, ELISA.",institutionString:null,institution:{name:"Ege University",country:{name:"Turkey"}}},{id:"246502",title:"Dr.",name:"Jaya T.",middleName:"T",surname:"Varkey",slug:"jaya-t.-varkey",fullName:"Jaya T. Varkey",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/246502/images/11160_n.jpg",biography:"Jaya T. Varkey, PhD, graduated with a degree in Chemistry from Cochin University of Science and Technology, Kerala, India. She obtained a PhD in Chemistry from the School of Chemical Sciences, Mahatma Gandhi University, Kerala, India, and completed a post-doctoral fellowship at the University of Minnesota, USA. She is a research guide at Mahatma Gandhi University and Associate Professor in Chemistry, St. Teresa’s College, Kochi, Kerala, India.\nDr. Varkey received a National Young Scientist award from the Indian Science Congress (1995), a UGC Research award (2016–2018), an Indian National Science Academy (INSA) Visiting Scientist award (2018–2019), and a Best Innovative Faculty award from the All India Association for Christian Higher Education (AIACHE) (2019). She Hashas received the Sr. Mary Cecil prize for best research paper three times. She was also awarded a start-up to develop a tea bag water filter. \nDr. Varkey has published two international books and twenty-seven international journal publications. She is an editorial board member for five international journals.",institutionString:"St. Teresa’s College",institution:null},{id:"250668",title:"Dr.",name:"Ali",middleName:null,surname:"Nabipour Chakoli",slug:"ali-nabipour-chakoli",fullName:"Ali Nabipour Chakoli",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/250668/images/system/250668.jpg",biography:"Academic Qualification:\r\n•\tPhD in Materials Physics and Chemistry, From: Sep. 2006, to: Sep. 2010, School of Materials Science and Engineering, Harbin Institute of Technology, Thesis: Structure and Shape Memory Effect of Functionalized MWCNTs/poly (L-lactide-co-ε-caprolactone) Nanocomposites. Supervisor: Prof. Wei Cai,\r\n•\tM.Sc in Applied Physics, From: 1996, to: 1998, Faculty of Physics & Nuclear Science, Amirkabir Uni. of Technology, Tehran, Iran, Thesis: Determination of Boron in Micro alloy Steels with solid state nuclear track detectors by neutron induced auto radiography, Supervisors: Dr. M. Hosseini Ashrafi and Dr. A. Hosseini.\r\n•\tB.Sc. in Applied Physics, From: 1991, to: 1996, Faculty of Physics & Nuclear Science, Amirkabir Uni. of Technology, Tehran, Iran, Thesis: Design of shielding for Am-Be neutron sources for In Vivo neutron activation analysis, Supervisor: Dr. M. Hosseini Ashrafi.\r\n\r\nResearch Experiences:\r\n1.\tNanomaterials, Carbon Nanotubes, Graphene: Synthesis, Functionalization and Characterization,\r\n2.\tMWCNTs/Polymer Composites: Fabrication and Characterization, \r\n3.\tShape Memory Polymers, Biodegradable Polymers, ORC, Collagen,\r\n4.\tMaterials Analysis and Characterizations: TEM, SEM, XPS, FT-IR, Raman, DSC, DMA, TGA, XRD, GPC, Fluoroscopy, \r\n5.\tInteraction of Radiation with Mater, Nuclear Safety and Security, NDT(RT),\r\n6.\tRadiation Detectors, Calibration (SSDL),\r\n7.\tCompleted IAEA e-learning Courses:\r\nNuclear Security (15 Modules),\r\nNuclear Safety:\r\nTSA 2: Regulatory Protection in Occupational Exposure,\r\nTips & Tricks: Radiation Protection in Radiography,\r\nSafety and Quality in Radiotherapy,\r\nCourse on Sealed Radioactive Sources,\r\nCourse on Fundamentals of Environmental Remediation,\r\nCourse on Planning for Environmental Remediation,\r\nKnowledge Management Orientation Course,\r\nFood Irradiation - Technology, Applications and Good Practices,\r\nEmployment:\r\nFrom 2010 to now: Academic staff, Nuclear Science and Technology Research Institute, Kargar Shomali, Tehran, Iran, P.O. Box: 14395-836.\r\nFrom 1997 to 2006: Expert of Materials Analysis and Characterization. Research Center of Agriculture and Medicine. Rajaeeshahr, Karaj, Iran, P. O. Box: 31585-498.",institutionString:"Atomic Energy Organization of Iran",institution:{name:"Atomic Energy Organization of Iran",country:{name:"Iran"}}},{id:"248279",title:"Dr.",name:"Monika",middleName:"Elzbieta",surname:"Machoy",slug:"monika-machoy",fullName:"Monika Machoy",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/248279/images/system/248279.jpeg",biography:"Monika Elżbieta Machoy, MD, graduated with distinction from the Faculty of Medicine and Dentistry at the Pomeranian Medical University in 2009, defended her PhD thesis with summa cum laude in 2016 and is currently employed as a researcher at the Department of Orthodontics of the Pomeranian Medical University. She expanded her professional knowledge during a one-year scholarship program at the Ernst Moritz Arndt University in Greifswald, Germany and during a three-year internship at the Technical University in Dresden, Germany. She has been a speaker at numerous orthodontic conferences, among others, American Association of Orthodontics, European Orthodontic Symposium and numerous conferences of the Polish Orthodontic Society. She conducts research focusing on the effect of orthodontic treatment on dental and periodontal tissues and the causes of pain in orthodontic patients.",institutionString:"Pomeranian Medical University",institution:{name:"Pomeranian Medical University",country:{name:"Poland"}}},{id:"252743",title:"Prof.",name:"Aswini",middleName:"Kumar",surname:"Kar",slug:"aswini-kar",fullName:"Aswini Kar",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/252743/images/10381_n.jpg",biography:"uploaded in cv",institutionString:null,institution:{name:"KIIT University",country:{name:"India"}}},{id:"204256",title:"Dr.",name:"Anil",middleName:"Kumar",surname:"Kumar Sahu",slug:"anil-kumar-sahu",fullName:"Anil Kumar Sahu",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/204256/images/14201_n.jpg",biography:"I have nearly 11 years of research and teaching experience. I have done my master degree from University Institute of Pharmacy, Pt. Ravi Shankar Shukla University, Raipur, Chhattisgarh India. I have published 16 review and research articles in international and national journals and published 4 chapters in IntechOpen, the world’s leading publisher of Open access books. I have presented many papers at national and international conferences. I have received research award from Indian Drug Manufacturers Association in year 2015. My research interest extends from novel lymphatic drug delivery systems, oral delivery system for herbal bioactive to formulation optimization.",institutionString:null,institution:{name:"Chhattisgarh Swami Vivekanand Technical University",country:{name:"India"}}},{id:"253468",title:"Dr.",name:"Mariusz",middleName:null,surname:"Marzec",slug:"mariusz-marzec",fullName:"Mariusz Marzec",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/253468/images/system/253468.png",biography:"An assistant professor at Department of Biomedical Computer Systems, at Institute of Computer Science, Silesian University in Katowice. Scientific interests: computer analysis and processing of images, biomedical images, databases and programming languages. He is an author and co-author of scientific publications covering analysis and processing of biomedical images and development of database systems.",institutionString:"University of Silesia",institution:null},{id:"212432",title:"Prof.",name:"Hadi",middleName:null,surname:"Mohammadi",slug:"hadi-mohammadi",fullName:"Hadi Mohammadi",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/212432/images/system/212432.jpeg",biography:"Dr. Hadi Mohammadi is a biomedical engineer with hands-on experience in the design and development of many engineering structures and medical devices through various projects that he has been involved in over the past twenty years. Dr. Mohammadi received his BSc. and MSc. degrees in Mechanical Engineering from Sharif University of Technology, Tehran, Iran, and his PhD. degree in Biomedical Engineering (biomaterials) from the University of Western Ontario. He was a postdoctoral trainee for almost four years at University of Calgary and Harvard Medical School. He is an industry innovator having created the technology to produce lifelike synthetic platforms that can be used for the simulation of almost all cardiovascular reconstructive surgeries. He’s been heavily involved in the design and development of cardiovascular devices and technology for the past 10 years. He is currently an Assistant Professor with the University of British Colombia, Canada.",institutionString:"University of British Columbia",institution:{name:"University of British Columbia",country:{name:"Canada"}}},{id:"254463",title:"Prof.",name:"Haisheng",middleName:null,surname:"Yang",slug:"haisheng-yang",fullName:"Haisheng Yang",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/254463/images/system/254463.jpeg",biography:"Haisheng Yang, Ph.D., Professor and Director of the Department of Biomedical Engineering, College of Life Science and Bioengineering, Beijing University of Technology. He received his Ph.D. degree in Mechanics/Biomechanics from Harbin Institute of Technology (jointly with University of California, Berkeley). Afterwards, he worked as a Postdoctoral Research Associate in the Purdue Musculoskeletal Biology and Mechanics Lab at the Department of Basic Medical Sciences, Purdue University, USA. He also conducted research in the Research Centre of Shriners Hospitals for Children-Canada at McGill University, Canada. Dr. Yang has over 10 years research experience in orthopaedic biomechanics and mechanobiology of bone adaptation and regeneration. He earned an award from Beijing Overseas Talents Aggregation program in 2017 and serves as Beijing Distinguished Professor.",institutionString:"Beijing University of Technology",institution:null},{id:"255757",title:"Dr.",name:"Igor",middleName:"Victorovich",surname:"Lakhno",slug:"igor-lakhno",fullName:"Igor Lakhno",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/255757/images/system/255757.jpg",biography:"Lakhno Igor Victorovich was born in 1971 in Kharkiv (Ukraine). \nMD – 1994, Kharkiv National Medical Univesity.\nOb&Gyn; – 1997, master courses in Kharkiv Medical Academy of Postgraduate Education.\nPhD – 1999, Kharkiv National Medical Univesity.\nDSc – 2019, PL Shupik National Academy of Postgraduate Education \nLakhno Igor has been graduated from an international training courses on reproductive medicine and family planning held in Debrecen University (Hungary) in 1997. Since 1998 Lakhno Igor has worked as an associate professor of the department of obstetrics and gynecology of VN Karazin National University and an associate professor of the perinatology, obstetrics and gynecology department of Kharkiv Medical Academy of Postgraduate Education. Since June 2019 he’s a professor of the department of obstetrics and gynecology of VN Karazin National University and a professor of the perinatology, obstetrics and gynecology department of Kharkiv Medical Academy of Postgraduate Education . He’s an author of about 200 printed works and there are 17 of them in Scopus or Web of Science databases. Lakhno Igor is a rewiever of Journal of Obstetrics and Gynaecology (Taylor and Francis), Informatics in Medicine Unlocked (Elsevier), The Journal of Obstetrics and Gynecology Research (Wiley), Endocrine, Metabolic & Immune Disorders-Drug Targets (Bentham Open), The Open Biomedical Engineering Journal (Bentham Open), etc. He’s defended a dissertation for DSc degree \\'Pre-eclampsia: prediction, prevention and treatment”. Lakhno Igor has participated as a speaker in several international conferences and congresses (International Conference on Biological Oscillations April 10th-14th 2016, Lancaster, UK, The 9th conference of the European Study Group on Cardiovascular Oscillations). His main scientific interests: obstetrics, women’s health, fetal medicine, cardiovascular medicine.",institutionString:"V.N. Karazin Kharkiv National University",institution:{name:"Kharkiv Medical Academy of Postgraduate Education",country:{name:"Ukraine"}}},{id:"89721",title:"Dr.",name:"Mehmet",middleName:"Cuneyt",surname:"Ozmen",slug:"mehmet-ozmen",fullName:"Mehmet Ozmen",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/89721/images/7289_n.jpg",biography:null,institutionString:null,institution:{name:"Gazi University",country:{name:"Turkey"}}},{id:"243698",title:"M.D.",name:"Xiaogang",middleName:null,surname:"Wang",slug:"xiaogang-wang",fullName:"Xiaogang Wang",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/243698/images/system/243698.png",biography:"Dr. Xiaogang Wang, a faculty member of Shanxi Eye Hospital specializing in the treatment of cataract and retinal disease and a tutor for postgraduate students of Shanxi Medical University, worked in the COOL Lab as an international visiting scholar under the supervision of Dr. David Huang and Yali Jia from October 2012 through November 2013. Dr. Wang earned an MD from Shanxi Medical University and a Ph.D. from Shanghai Jiao Tong University. Dr. Wang was awarded two research project grants focused on multimodal optical coherence tomography imaging and deep learning in cataract and retinal disease, from the National Natural Science Foundation of China. He has published around 30 peer-reviewed journal papers and four book chapters and co-edited one book.",institutionString:"Shanxi Eye Hospital",institution:{name:"Shanxi Eye Hospital",country:{name:"China"}}},{id:"242893",title:"Ph.D. Student",name:"Joaquim",middleName:null,surname:"De Moura",slug:"joaquim-de-moura",fullName:"Joaquim De Moura",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/242893/images/7133_n.jpg",biography:"Joaquim de Moura received his degree in Computer Engineering in 2014 from the University of A Coruña (Spain). In 2016, he received his M.Sc degree in Computer Engineering from the same university. He is currently pursuing his Ph.D degree in Computer Science in a collaborative project between ophthalmology centers in Galicia and the University of A Coruña. His research interests include computer vision, machine learning algorithms and analysis and medical imaging processing of various kinds.",institutionString:null,institution:{name:"University of A Coruña",country:{name:"Spain"}}},{id:"267434",title:"Dr.",name:"Rohit",middleName:null,surname:"Raja",slug:"rohit-raja",fullName:"Rohit Raja",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRZkkQAG/Profile_Picture_2022-05-09T12:55:18.jpg",biography:null,institutionString:null,institution:null},{id:"294334",title:"B.Sc.",name:"Marc",middleName:null,surname:"Bruggeman",slug:"marc-bruggeman",fullName:"Marc Bruggeman",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/294334/images/8242_n.jpg",biography:"Chemical engineer graduate, with a passion for material science and specific interest in polymers - their near infinite applications intrigue me. \n\nI plan to continue my scientific career in the field of polymeric biomaterials as I am fascinated by intelligent, bioactive and biomimetic materials for use in both consumer and medical applications.",institutionString:null,institution:null},{id:"244950",title:"Dr.",name:"Salvatore",middleName:null,surname:"Di Lauro",slug:"salvatore-di-lauro",fullName:"Salvatore Di Lauro",position:null,profilePictureURL:"https://intech-files.s3.amazonaws.com/0030O00002bSF1HQAW/ProfilePicture%202021-12-20%2014%3A54%3A14.482",biography:"Name:\n\tSALVATORE DI LAURO\nAddress:\n\tHospital Clínico Universitario Valladolid\nAvda Ramón y Cajal 3\n47005, Valladolid\nSpain\nPhone number: \nFax\nE-mail:\n\t+34 983420000 ext 292\n+34 983420084\nsadilauro@live.it\nDate and place of Birth:\nID Number\nMedical Licence \nLanguages\t09-05-1985. Villaricca (Italy)\n\nY1281863H\n474707061\nItalian (native language)\nSpanish (read, written, spoken)\nEnglish (read, written, spoken)\nPortuguese (read, spoken)\nFrench (read)\n\t\t\nCurrent position (title and company)\tDate (Year)\nVitreo-Retinal consultant in ophthalmology. Hospital Clinico Universitario Valladolid. Sacyl. National Health System.\nVitreo-Retinal consultant in ophthalmology. Instituto Oftalmologico Recoletas. Red Hospitalaria Recoletas. Private practise.\t2017-today\n\n2019-today\n\t\n\t\nEducation (High school, university and postgraduate training > 3 months)\tDate (Year)\nDegree in Medicine and Surgery. University of Neaples 'Federico II”\nResident in Opthalmology. Hospital Clinico Universitario Valladolid\nMaster in Vitreo-Retina. IOBA. University of Valladolid\nFellow of the European Board of Ophthalmology. Paris\nMaster in Research in Ophthalmology. University of Valladolid\t2003-2009\n2012-2016\n2016-2017\n2016\n2012-2013\n\t\nEmployments (company and positions)\tDate (Year)\nResident in Ophthalmology. Hospital Clinico Universitario Valladolid. Sacyl.\nFellow in Vitreo-Retina. IOBA. University of Valladolid\nVitreo-Retinal consultant in ophthalmology. Hospital Clinico Universitario Valladolid. Sacyl. National Health System.\nVitreo-Retinal consultant in ophthalmology. Instituto Oftalmologico Recoletas. Red Hospitalaria Recoletas. \n\t2012-2016\n2016-2017\n2017-today\n\n2019-Today\n\n\n\t\nClinical Research Experience (tasks and role)\tDate (Year)\nAssociated investigator\n\n' FIS PI20/00740: DESARROLLO DE UNA CALCULADORA DE RIESGO DE\nAPARICION DE RETINOPATIA DIABETICA BASADA EN TECNICAS DE IMAGEN MULTIMODAL EN PACIENTES DIABETICOS TIPO 1. Grant by: Ministerio de Ciencia e Innovacion \n\n' (BIO/VA23/14) Estudio clínico multicéntrico y prospectivo para validar dos\nbiomarcadores ubicados en los genes p53 y MDM2 en la predicción de los resultados funcionales de la cirugía del desprendimiento de retina regmatógeno. Grant by: Gerencia Regional de Salud de la Junta de Castilla y León.\n' Estudio multicéntrico, aleatorizado, con enmascaramiento doble, en 2 grupos\nparalelos y de 52 semanas de duración para comparar la eficacia, seguridad e inmunogenicidad de SOK583A1 respecto a Eylea® en pacientes con degeneración macular neovascular asociada a la edad' (CSOK583A12301; N.EUDRA: 2019-004838-41; FASE III). Grant by Hexal AG\n\n' Estudio de fase III, aleatorizado, doble ciego, con grupos paralelos, multicéntrico para comparar la eficacia y la seguridad de QL1205 frente a Lucentis® en pacientes con degeneración macular neovascular asociada a la edad. (EUDRACT: 2018-004486-13). Grant by Qilu Pharmaceutical Co\n\n' Estudio NEUTON: Ensayo clinico en fase IV para evaluar la eficacia de aflibercept en pacientes Naive con Edema MacUlar secundario a Oclusion de Vena CenTral de la Retina (OVCR) en regimen de tratamientO iNdividualizado Treat and Extend (TAE)”, (2014-000975-21). Grant by Fundacion Retinaplus\n\n' Evaluación de la seguridad y bioactividad de anillos de tensión capsular en conejo. Proyecto Procusens. Grant by AJL, S.A.\n\n'Estudio epidemiológico, prospectivo, multicéntrico y abierto\\npara valorar la frecuencia de la conjuntivitis adenovírica diagnosticada mediante el test AdenoPlus®\\nTest en pacientes enfermos de conjuntivitis aguda”\\n. National, multicenter study. Grant by: NICOX.\n\nEuropean multicentric trial: 'Evaluation of clinical outcomes following the use of Systane Hydration in patients with dry eye”. Study Phase 4. Grant by: Alcon Labs'\n\nVLPs Injection and Activation in a Rabbit Model of Uveal Melanoma. Grant by Aura Bioscience\n\nUpdating and characterization of a rabbit model of uveal melanoma. Grant by Aura Bioscience\n\nEnsayo clínico en fase IV para evaluar las variantes genéticas de la vía del VEGF como biomarcadores de eficacia del tratamiento con aflibercept en pacientes con degeneración macular asociada a la edad (DMAE) neovascular. Estudio BIOIMAGE. IMO-AFLI-2013-01\n\nEstudio In-Eye:Ensayo clínico en fase IV, abierto, aleatorizado, de 2 brazos,\nmulticçentrico y de 12 meses de duración, para evaluar la eficacia y seguridad de un régimen de PRN flexible individualizado de 'esperar y extender' versus un régimen PRN según criterios de estabilización mediante evaluaciones mensuales de inyecciones intravítreas de ranibizumab 0,5 mg en pacientes naive con neovascularización coriodea secunaria a la degeneración macular relacionada con la edad. CP: CRFB002AES03T\n\nTREND: Estudio Fase IIIb multicéntrico, randomizado, de 12 meses de\nseguimiento con evaluador de la agudeza visual enmascarado, para evaluar la eficacia y la seguridad de ranibizumab 0.5mg en un régimen de tratar y extender comparado con un régimen mensual, en pacientes con degeneración macular neovascular asociada a la edad. CP: CRFB002A2411 Código Eudra CT:\n2013-002626-23\n\n\n\nPublications\t\n\n2021\n\n\n\n\n2015\n\n\n\n\n2021\n\n\n\n\n\n2021\n\n\n\n\n2015\n\n\n\n\n2015\n\n\n2014\n\n\n\n\n2015-16\n\n\n\n2015\n\n\n2014\n\n\n2014\n\n\n\n\n2014\n\n\n\n\n\n\n\n2014\n\nJose Carlos Pastor; Jimena Rojas; Salvador Pastor-Idoate; Salvatore Di Lauro; Lucia Gonzalez-Buendia; Santiago Delgado-Tirado. Proliferative vitreoretinopathy: A new concept of disease pathogenesis and practical\nconsequences. Progress in Retinal and Eye Research. 51, pp. 125 - 155. 03/2016. DOI: 10.1016/j.preteyeres.2015.07.005\n\n\nLabrador-Velandia S; Alonso-Alonso ML; Di Lauro S; García-Gutierrez MT; Srivastava GK; Pastor JC; Fernandez-Bueno I. Mesenchymal stem cells provide paracrine neuroprotective resources that delay degeneration of co-cultured organotypic neuroretinal cultures.Experimental Eye Research. 185, 17/05/2019. DOI: 10.1016/j.exer.2019.05.011\n\nSalvatore Di Lauro; Maria Teresa Garcia Gutierrez; Ivan Fernandez Bueno. Quantification of pigment epithelium-derived factor (PEDF) in an ex vivo coculture of retinal pigment epithelium cells and neuroretina.\nJournal of Allbiosolution. 2019. ISSN 2605-3535\n\nSonia Labrador Velandia; Salvatore Di Lauro; Alonso-Alonso ML; Tabera Bartolomé S; Srivastava GK; Pastor JC; Fernandez-Bueno I. Biocompatibility of intravitreal injection of human mesenchymal stem cells in immunocompetent rabbits. Graefe's archive for clinical and experimental ophthalmology. 256 - 1, pp. 125 - 134. 01/2018. DOI: 10.1007/s00417-017-3842-3\n\n\nSalvatore Di Lauro, David Rodriguez-Crespo, Manuel J Gayoso, Maria T Garcia-Gutierrez, J Carlos Pastor, Girish K Srivastava, Ivan Fernandez-Bueno. A novel coculture model of porcine central neuroretina explants and retinal pigment epithelium cells. Molecular Vision. 2016 - 22, pp. 243 - 253. 01/2016.\n\nSalvatore Di Lauro. Classifications for Proliferative Vitreoretinopathy ({PVR}): An Analysis of Their Use in Publications over the Last 15 Years. Journal of Ophthalmology. 2016, pp. 1 - 6. 01/2016. DOI: 10.1155/2016/7807596\n\nSalvatore Di Lauro; Rosa Maria Coco; Rosa Maria Sanabria; Enrique Rodriguez de la Rua; Jose Carlos Pastor. Loss of Visual Acuity after Successful Surgery for Macula-On Rhegmatogenous Retinal Detachment in a Prospective Multicentre Study. Journal of Ophthalmology. 2015:821864, 2015. DOI: 10.1155/2015/821864\n\nIvan Fernandez-Bueno; Salvatore Di Lauro; Ivan Alvarez; Jose Carlos Lopez; Maria Teresa Garcia-Gutierrez; Itziar Fernandez; Eva Larra; Jose Carlos Pastor. Safety and Biocompatibility of a New High-Density Polyethylene-Based\nSpherical Integrated Porous Orbital Implant: An Experimental Study in Rabbits. Journal of Ophthalmology. 2015:904096, 2015. DOI: 10.1155/2015/904096\n\nPastor JC; Pastor-Idoate S; Rodríguez-Hernandez I; Rojas J; Fernandez I; Gonzalez-Buendia L; Di Lauro S; Gonzalez-Sarmiento R. Genetics of PVR and RD. Ophthalmologica. 232 - Suppl 1, pp. 28 - 29. 2014\n\nRodriguez-Crespo D; Di Lauro S; Singh AK; Garcia-Gutierrez MT; Garrosa M; Pastor JC; Fernandez-Bueno I; Srivastava GK. Triple-layered mixed co-culture model of RPE cells with neuroretina for evaluating the neuroprotective effects of adipose-MSCs. Cell Tissue Res. 358 - 3, pp. 705 - 716. 2014.\nDOI: 10.1007/s00441-014-1987-5\n\nCarlo De Werra; Salvatore Condurro; Salvatore Tramontano; Mario Perone; Ivana Donzelli; Salvatore Di Lauro; Massimo Di Giuseppe; Rosa Di Micco; Annalisa Pascariello; Antonio Pastore; Giorgio Diamantis; Giuseppe Galloro. Hydatid disease of the liver: thirty years of surgical experience.Chirurgia italiana. 59 - 5, pp. 611 - 636.\n(Italia): 2007. ISSN 0009-4773\n\nChapters in books\n\t\n' Salvador Pastor Idoate; Salvatore Di Lauro; Jose Carlos Pastor Jimeno. PVR: Pathogenesis, Histopathology and Classification. Proliferative Vitreoretinopathy with Small Gauge Vitrectomy. Springer, 2018. ISBN 978-3-319-78445-8\nDOI: 10.1007/978-3-319-78446-5_2. \n\n' Salvatore Di Lauro; Maria Isabel Lopez Galvez. Quistes vítreos en una mujer joven. Problemas diagnósticos en patología retinocoroidea. Sociedad Española de Retina-Vitreo. 2018.\n\n' Salvatore Di Lauro; Salvador Pastor Idoate; Jose Carlos Pastor Jimeno. iOCT in PVR management. OCT Applications in Opthalmology. pp. 1 - 8. INTECH, 2018. DOI: 10.5772/intechopen.78774.\n\n' Rosa Coco Martin; Salvatore Di Lauro; Salvador Pastor Idoate; Jose Carlos Pastor. amponadores, manipuladores y tinciones en la cirugía del traumatismo ocular.Trauma Ocular. Ponencia de la SEO 2018..\n\n' LOPEZ GALVEZ; DI LAURO; CRESPO. OCT angiografia y complicaciones retinianas de la diabetes. PONENCIA SEO 2021, CAPITULO 20. (España): 2021.\n\n' Múltiples desprendimientos neurosensoriales bilaterales en paciente joven. Enfermedades Degenerativas De Retina Y Coroides. SERV 04/2016. \n' González-Buendía L; Di Lauro S; Pastor-Idoate S; Pastor Jimeno JC. Vitreorretinopatía proliferante (VRP) e inflamación: LA INFLAMACIÓN in «INMUNOMODULADORES Y ANTIINFLAMATORIOS: MÁS ALLÁ DE LOS CORTICOIDES. RELACION DE PONENCIAS DE LA SOCIEDAD ESPAÑOLA DE OFTALMOLOGIA. 10/2014.",institutionString:null,institution:null},{id:"265335",title:"Mr.",name:"Stefan",middleName:"Radnev",surname:"Stefanov",slug:"stefan-stefanov",fullName:"Stefan Stefanov",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/265335/images/7562_n.jpg",biography:null,institutionString:null,institution:null},{id:"318905",title:"Prof.",name:"Elvis",middleName:"Kwason",surname:"Tiburu",slug:"elvis-tiburu",fullName:"Elvis Tiburu",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"University of Ghana",country:{name:"Ghana"}}},{id:"336193",title:"Dr.",name:"Abdullah",middleName:null,surname:"Alamoudi",slug:"abdullah-alamoudi",fullName:"Abdullah Alamoudi",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Majmaah University",country:{name:"Saudi Arabia"}}},{id:"318657",title:"MSc.",name:"Isabell",middleName:null,surname:"Steuding",slug:"isabell-steuding",fullName:"Isabell Steuding",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Harz University of Applied Sciences",country:{name:"Germany"}}},{id:"318656",title:"BSc.",name:"Peter",middleName:null,surname:"Kußmann",slug:"peter-kussmann",fullName:"Peter Kußmann",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Harz University of Applied Sciences",country:{name:"Germany"}}},{id:"338222",title:"Mrs.",name:"María José",middleName:null,surname:"Lucía Mudas",slug:"maria-jose-lucia-mudas",fullName:"María José Lucía Mudas",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Carlos III University of Madrid",country:{name:"Spain"}}},{id:"147824",title:"Mr.",name:"Pablo",middleName:null,surname:"Revuelta Sanz",slug:"pablo-revuelta-sanz",fullName:"Pablo Revuelta Sanz",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Carlos III University of Madrid",country:{name:"Spain"}}}]}},subseries:{item:{id:"12",type:"subseries",title:"Human Physiology",keywords:"Anatomy, Cells, Organs, Systems, Homeostasis, Functions",scope:"Human physiology is the scientific exploration of the various functions (physical, biochemical, and mechanical properties) of humans, their organs, and their constituent cells. The endocrine and nervous systems play important roles in maintaining homeostasis in the human body. Integration, which is the biological basis of physiology, is achieved through communication between the many overlapping functions of the human body's systems, which takes place through electrical and chemical means. Much of the basis of our knowledge of human physiology has been provided by animal experiments. Because of the close relationship between structure and function, studies in human physiology and anatomy seek to understand the mechanisms that help the human body function. The series on human physiology deals with the various mechanisms of interaction between the various organs, nerves, and cells in the human body.",coverUrl:"https://cdn.intechopen.com/series_topics/covers/12.jpg",hasOnlineFirst:!0,hasPublishedBooks:!0,annualVolume:11408,editor:{id:"195829",title:"Prof.",name:"Kunihiro",middleName:null,surname:"Sakuma",slug:"kunihiro-sakuma",fullName:"Kunihiro Sakuma",profilePictureURL:"https://mts.intechopen.com/storage/users/195829/images/system/195829.jpg",biography:"Professor Kunihiro Sakuma, Ph.D., currently works in the Institute for Liberal Arts at the Tokyo Institute of Technology. He is a physiologist working in the field of skeletal muscle. He was awarded his sports science diploma in 1995 by the University of Tsukuba and began his scientific work at the Department of Physiology, Aichi Human Service Center, focusing on the molecular mechanism of congenital muscular dystrophy and normal muscle regeneration. 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Topics include, but are not limited to: Advanced techniques of cellular and molecular biology (Molecular methodologies, imaging techniques, and bioinformatics); Biological activities at the molecular level; Biological processes of cell functions, cell division, senescence, maintenance, and cell death; Biomolecules interactions; Cancer; Cell biology; Chemical biology; Computational biology; Cytochemistry; Developmental biology; Disease mechanisms and therapeutics; DNA, and RNA metabolism; Gene functions, genetics, and genomics; Genetics; Immunology; Medical microbiology; Molecular biology; Molecular genetics; Molecular processes of cell and organelle dynamics; Neuroscience; Protein biosynthesis, degradation, and functions; Regulation of molecular interactions in a cell; Signalling networks and system biology; Structural biology; Virology and microbiology.",annualVolume:11410,isOpenForSubmission:!0,coverUrl:"https://cdn.intechopen.com/series_topics/covers/14.jpg",editor:{id:"165627",title:"Dr.",name:"Rosa María",middleName:null,surname:"Martínez-Espinosa",fullName:"Rosa María Martínez-Espinosa",profilePictureURL:"https://mts.intechopen.com/storage/users/165627/images/system/165627.jpeg",institutionString:null,institution:{name:"University of Alicante",institutionURL:null,country:{name:"Spain"}}},editorTwo:null,editorThree:null,editorialBoard:[{id:"79367",title:"Dr.",name:"Ana Isabel",middleName:null,surname:"Flores",fullName:"Ana Isabel Flores",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRpIOQA0/Profile_Picture_1632418099564",institutionString:null,institution:{name:"Hospital Universitario 12 De Octubre",institutionURL:null,country:{name:"Spain"}}},{id:"328234",title:"Ph.D.",name:"Christian",middleName:null,surname:"Palavecino",fullName:"Christian Palavecino",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y000030DhEhQAK/Profile_Picture_1628835318625",institutionString:null,institution:{name:"Central University of Chile",institutionURL:null,country:{name:"Chile"}}},{id:"186585",title:"Dr.",name:"Francisco Javier",middleName:null,surname:"Martin-Romero",fullName:"Francisco Javier Martin-Romero",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bSB3HQAW/Profile_Picture_1631258137641",institutionString:null,institution:{name:"University of Extremadura",institutionURL:null,country:{name:"Spain"}}}]},{id:"15",title:"Chemical Biology",keywords:"Phenolic Compounds, Essential Oils, Modification of Biomolecules, Glycobiology, Combinatorial Chemistry, Therapeutic peptides, Enzyme Inhibitors",scope:"Chemical biology spans the fields of chemistry and biology involving the application of biological and chemical molecules and techniques. 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Behind these definitions are hidden all the aspects of normal and pathological functioning of all processes that the topic ‘Metabolism’ will cover within the Biochemistry Series. 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Thus proteomics, an area of research that detects all protein forms expressed in an organism, including splice isoforms and post-translational modifications, is more suitable than genomics for a comprehensive understanding of the biochemical processes that govern life. The most common proteomics applications are currently in the clinical field for the identification, in a variety of biological matrices, of biomarkers for diagnosis and therapeutic intervention of disorders. From the comparison of proteomic profiles of control and disease or different physiological states, which may emerge, changes in protein expression can provide new insights into the roles played by some proteins in human pathologies. Understanding how proteins function and interact with each other is another goal of proteomics that makes this approach even more intriguing. Specialized technology and expertise are required to assess the proteome of any biological sample. 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