Open access peer-reviewed chapter

Opportunity of Non-Wood Forest Products in Biocomposites

Written By

Pradeep Sharma

Submitted: 19 March 2021 Reviewed: 21 April 2021 Published: 29 May 2021

DOI: 10.5772/intechopen.97825

From the Edited Volume


Edited by Brajesh Kumar

Chapter metrics overview

322 Chapter Downloads

View Full Metrics


In recent years industries are attempting to decrease their dependency on petroleum-based fuels and products due to increased environmental issues. The tremendous increase in production and use of plastics in every sector of life has led to huge plastic waste disposal problems and also an environmental threat. In order to prevail over the present scenario, the viable and cost-effective approaches are to prepare eco-friendly bio-composites based on non-wood forest products (NWFP), a part of forest wealth of the globe, especially natural fibres, agricultural wastes and extractives. Natural fibres and extractives have many advantages viz. low density, low cost, considerable toughness properties, nontoxicity, sustainability and biodegradability. NWFP based composites may be utilized to produce non-structural parts for diverse applications in various industries as high-performance materials with interesting properties for specific applications viz. furniture, thermal, acoustic insulations and automotive industries etc. In the present chapter, opportunities of extractives, cellulosic and lignocellulosic fibres from non-wood forest products in Bio-composites will be discussed.


  • Non-wood forest products
  • Bio-composites
  • Tannins
  • Laccase
  • Fibres
  • Agriculture wastes

1. Introduction

Biocomposites are composites formed by the mixture of two or more than two constituents which are firmly stacked in a specific orientation in order to provide stability and toughness as per their requirement. Composites may be synthetic, biocomposites or natural composites. Natural composites are the wood, bamboo, silk, cellulose, and some animal products viz. feather, horn etc. Bio-composites are materials formed by reinforcement of natural fibres into adhesive or a matrix. The matrix may be a natural, synthetic material or an amalgamation of natural and synthetic materials. Environmental concerns over the synthetic matrix and further cost of synthetic fibres have led the encouragement of scientific community of using natural fibres as reinforcement material in polymeric composites.


2. Non wood forest products

The non wood forest products (nwfps) comprise all the forest products other than timber and fuelwood and are used by human beings since the time immemorial [1, 2, 3]. NWFPs include medicinal plants, essential oils, spices, edible wild plants, gums, resins and oleoresins, fatty oils, tanning materials, natural organic colouring materials, katha and cutch, oxalic acid, fibres and flosses, beverages and narcotics, fodder and forage plants, saponins, fish poisons, insecticides, green manure, beads, rubber plants, plants useful for paper, basket and wicker work including canes, beedi leaf etc., miscellaneous materials including thatching and broom materials. Besides these plant products, animal products such as lac, honey, silk, horns, ivory and hides (of forest origin) are included among the nwfps [4, 5, 6]. Developing countries especially tropical region of the world more than three-fourths of the populations are dependent on nwfps for their nutrition, primary health care and livelihood subsistence. Therefore, nwfps play an important role in the daily lives of local population in particular rural and poor people dependency on nwfps for their daily needs of food, fodder, medicines, gums, construction material, etc. In addition to local consumption, nwfps are also traded in local, regional, national and international markets and the trade in nwfps not only generates employment opportunities but also contributes in the economic development of the country [7, 8, 9]. Among the nwfps fibres and flosses, bamboo and canes, tans and dyes, essential oils are important forest bio-products for livelihood support to marginal peoples residing in forest areas. After processing of the essential products (dyes, essential oils etc.), the left over biomass may be utilized for diverse industrial applications. The importance of these nwfps lies in the following facts.

2.1 Fibres and flosses

A wide range of plants yielding fibre occur in the forests wealth. Fibres are obtained from tissues of different parts of certain woody plants, which are used for various traditional applications such as making cloth, rope, mat and cordages etc. [10, 11].

2.2 Bamboo

Bamboo is abundantly found in most parts of the world, nearly 0.92 % of the total forest area, spread over 36 million hectares (MHa) [12]. Globally, bamboo has 111 genera with more than 1575 species. India is very rich in bamboo resources and the second major bamboo producing country having 16 MHa (22.46 %) of a total forest cover 71.2 MHa [13] comprising of 160 species after China. China is the richest in bamboo resources; it has more than 800 species [14, 15]. The bamboo is used for various industrial purposes [16, 17, 18]. Bamboo species thrives in almost all types of soil except in very dry soils. The bamboo is utilized for various purposes, viz. agriculture, handicraft, building industry (bamboo concrete, scaffoldings, house construction, etc.), interior decoration (Bamboo flooring board, mat, panelling, curtain, etc.), paper industry, textile industry, food, bamboo charcoal, and diverse range of daily use articles (toothpicks, chopsticks, incense sticks, etc.) [19, 20, 21, 22]. Bamboo is a typical natural composite material with functionally gradient structure having multi-nodes, and the fibres are arranged compactly in the outer surface region in a definite fashion in comparison to inner surface region which provides fracture toughness. The fracture toughness of the bamboo culm depends on the volume fraction of fibres [23, 24, 25, 26, 27, 28].

2.3 Essential oils

Essential oils from plants are widely used in pharmaceuticals, cosmetics, perfumery and specialty applications in various industries. The value of essential oil bearing crops can be augmented by utilization of wastes using facile and economic methods. The major essential oil bearing crops are lemon grass, mentha, eucalyptus, lavender, rose, geranium, rosemary, basil, thyme, peppermint, chamomile, etc. [5].

2.4 Tans and dyes

A variety of vegetable tanning materials are produced in the forests. Important vegetable tanning materials are the myrobalan nuts and bark of wattle (Acacia mearnsii, A. decurrens, A. nilotica and Cassia auriculata, etc.). Other tanning materials include leaves of Emblica officinalis and Anogeissus latifolia, bark of Cleistanthus collinus, fruits of Zizyphus xylocarpa, bark of Cassia fistula, Terminalia alata, T. arjuna, etc. The term tannin was introduced by Seguin in the year 1796 to indicate various plant extracts, which have the capacity to convert hides and skins into leather [5, 29, 30]. Tans and dyes are simple chemical compounds made of carbon, hydrogen and oxygen. The structure and solubility of tannins are dependent upon the source and structure of tannins, however, vegetable tannins are water-soluble.

2.5 Wood tans

The important species yielding wood tans are Quebracho (Quebracho Colorado), this species is widely distributed in South America. The heart-wood contains 20-27 % tannin, which is obtained by cutting the wood into small chips and extracting the tannin with water. The cutch, a byproduct from Katha industry, obtained from khair (Acacia catechu) heart wood is used for tanning purposes.

2.6 Bark tans

The bark of various tree species is chipped off during the operation of timber or fuel-wood harvesting, some of the important species which yield bark tans are: Acacia mearnsii, Acacia nilotica, Cassia auriculata, Shorea robusta, Terminalia arjuna, Cassia fistula, Ceriops roxburghiana, Rhizophora mucronata, etc. Some other trees such as Acacia leucophloea, Bridelia retusa, Lagerstroemia spp., Tamarix aphylla, Terminalia alata, Quercus spp. and Castanopsis spp. also yield bark tans and are locally important.

2.7 Fruit tans

Fruits of some of the forest trees are utilized in tanning industries for extraction of different tannins. Some of the important species yielding fruit tans are as follows: Acacia nilotica (Babul), Caesalpinia coriaria (Divi-divi), Zizyphus xylopyrus (Kath bor), Emblica officinalis (Aonla), Shorea robusta (Sal), Anacardium occidentale (Cashew nut), Tamarindus indica (Tamarind) and Terminalia chebula (Myrobalan), etc.

2.8 Leaf tans

Leaves of some of plants provide tanning material however; they are not used on a large scale for commercial applications. Generally, village artisans and shoemakers use leaves for tanning leather on as a small scale. Important leaf tanning materials are obtained from leaves of Anogeissus latifolia, Carissa spinarum, Emblica officinalis, Lawsonia inermis and Rhus cotinus, etc.

2.9 Natural dyes

Natural dyes are broadly classified as plant, animal, mineral, and microbial dyes. They are obtained from the vegetable plant materials such as plant leaves, roots, bark, seeds, and from insect secretions and minerals. Dyes are used for various industrial applications including the coloring of textiles [31, 32].


3. Natural composites

Wood and bamboo are the natural composites, held together by the matrix as designed by the nature (Figure 1). Lignin is the largest biopolymer and principle cementing matrix which holds the components of natural composites together in a definite fashion. The awareness in the society globally and harmful effects of the synthetic materials on the environment has led to the progressive development of eco-friendly and sustainable materials. The scientific community have shown a lot of interest in developing sustainable bio-composites which are eco-friendly and may substitute partly or wholly the synthetic materials. Intriguingly, using natural fibres in development of biocomposites provides a reduction in greenhouse gas emissions and carbon footprint of composites [33, 34]. Due to environmental concern, demand for commercial raw material for utilization in composites increasing day by day. Therefore, after harvesting the important chemicals or fresh materials from the nwfps may be utilized on sustainable basis for making bio-composites for various industrial sectors. Among them, use of the natural fibres as reinforcement material, tannins and lignin as cementing material alone, in parts or in combination with synthetic matrix subsequent to proper modification, and further, valorisation of residues from the nwfps is an important opportunity as raw materials for bio composites in a sustainable way.

Figure 1.

Natural Wood Composite.


4. Natural fibres and their utilization in bio-composites

A vast number of research papers and reviews are available for utilization of natural fibres in sustainable development of biocomposites. However, as mentioned, the fibres are sustainable raw materials and may be obtained from different sources from nwfps, further, low-cost, light-weight, availability, renewability, biodegradability; properties and strength are important factors for their utilization. The specific properties of the fibres are utmost important in developing the composites. Natural fibres comprise of cellulose, hemicelluloses, lignin, waxes and tannins etc. The percentage of these constituents varies with the source and processing of fibres. Further, the properties of biocomposites are also dependent upon the source of fibres and presence of these constituents. Natural fibres are obtained from plants or animals [35]. The plant fibres are commonly used for producing bio-composites and mostly sourced from nwfps or in some countries cultivation of these crops is being carried out for sustainable production. The commercial sources of fibres utilized in producing biocomposites worldwide due to their inherent properties. The classification of fibres based on source, their specific properties and utilization in biocomposites are summarised in Tables 1-3.

Fibres SourceCommon namePart of the plantAppearenceDiameter (μm)Density (g/cm3)Tensile strength (MPa)Young’ modulus (GPa)
Agave sislanaSisalLeafCoarse-stiff; Creamy-White50-2001.45468-6409.4-22
Corhorus capsularisJute fibreBastFine Light brown25-2001.3-1.45393-77313-26.5
Cocos nuciferaCoconut fibre or CoirCoconut fruit outer shell or huskCourse, white to brown100-4501.15131-1754-6
Arenga pinnataSugar-palm fibreBast, fruit, leafCoarse, brownish- black50-8001.29190.293.69
Cannabis sativaHemp fibreBastSilky-fine, white to light brown26.51.4851424.8
Bamboo SpeciesBambooGrassCreamy white10-4050335.91
Linum usitatissimumFlax fibreBastCreamy white12-161.50345-110027.6
Hibiscus cannabinusKneaf fibreStemPale17.7-21.91.2-1.4
Boehmeria niveaRamie fibreBastWhite25-301.5-1.55400-160044
Gossypium Sp.Cotton fibreSeedsYellowish off-white11-221.5140012
ConifersSoft wood fibreSoft wood tracheidsVariation in colour20-351.5100040
Ananas comosusPine appleLeafWhite, smooth and glossy8.66-63.431.44413-162734.5-82.5

Table 1.

Plant fibres and properties [36, 37, 38, 39, 40, 41, 42, 43, 44, 45].

FibreCellulose (%)Hemicelluloses (%)Lignin (%)Pectin (%)
Pine apple80–8116–194.6–122–3
Kenaf49-53 (alpha)86.8-87.7 (Holocellulose)14.7-21.2-
Lemom grass71.79.5213.83-
Hard wood43-4716-2425-35-
Soft wood40-4425-3125-29

Table 2.

Chemistries of important plant fibers [43, 46, 47, 48, 49, 50].

Type of FibresSourcePlant of fibresUseProperties
Bast fibresStemJuteReinforcementsLong length high stiffness and strength
StemRamieReinforcementsLong length high stiffness and strength
StalkBambooBamboo speciesReinforcementShort and long fibres
WoodSoft and hard woodReinforcementShort fibres
LeafLeafBananaReinforcementLong fibres
Leafand sisalReinforcementLong fibres
SeedsCottonCotton speciesReinforcementShort fibres
Agricultural wastesCereals etc.ReinforcementShort and long fibres

Table 3.

Plant based fibres, source and utilization in biocomposites.

Natural fibres show a variation in properties. The fibre properties are dependent on the geographical location, process of isolation (ratting of fibres) and maturation period. However, some fibres exhibit highest tensile strength in a range from 300-1100 MPa (Table 1).

4.1 Merits and demerits of natural fibres

The inherent properties of the natural fibres of plants origin are important in developing the bio-composites. Natural fibres comprise of cellulose, hemicelluloses, lignin, waxes and tannins etc. The percentage of the cellulose, hemicelluloses, lignin etc., length and width of the fibres, varies with the source and processing of fibres. Further, natural fibres possess low density (1.25–1.50 g/cm3), sufficient mechanical properties, sustainability, recyclability, biodegradability, availability and low-priced in comparison to synthetic fibres such as glass and carbon fibres [51, 52]. Intriguingly, these properties do not meet the requirements of biocomposites. The natural fibres are used for increasing the mechanical strength as reinforcement material in composites [53]. The synthetic matrix and natural fibres are not compatible to each other leading to poor mechanical properties properties. Further, fibres have also water absorption capacity of cellulose due the presence of numerous hydroxyl groups [54, 55, 56].

Natural fibres are used as reinforcement materials in composites. However, due to their susceptibility to moisture [56] mechanical properties of polymeric composites have a strong impact on the interface adhesion between the fiber and the polymer matrix [54]. The natural fibres are rich of cellulose, hemicelluloses, lignin, pectins, waxes and tannins etc, all of which are composed of hydroxyl groups. Thus, there are major challenges of suitability between the matrix and fiber that weakens interface region between matrices and natural fibres [55]. Generally, outer surface of the composite materials absorb water and decreases gradually into the bulk of the matrix. High water absorption capacity of the composite materials leads to decline in their mechanical strength and pressure on nearby structures due to absorption of water pertaining to the hygroscopic nature of the fibres and subsequently can cause warping, buckling, bigger possibility of their microbial inhabitation, freeze, and unfreeze leading to destruction of mechanical characteristics of composite materials. Therefore, fibres are required to improve these limitations by physical and chemical modifications [57].

4.2 Alteration of properties of natural fibres

The compatibility of the natural plant fibres with the synthetic matrix is the main and foremost concern of developing bio-composites due to the different nature and properties of these two materials. The various methods have been studied and reviewed in the past in order to increase the functionality and compatibility of natural fibres. Fibres compatibility with the matrix and mechanical strength thereof may be increased by physical and chemical modification of the fibres.

4.2.1 Physical modification

The surface properties may be increased by physical treatments of the fibres. However, during extraction process, the journey of fibres to a final destination also involves the multi stepping process leading to stress and physical changes in the inherent properties of fibres. During the extraction process there are some fibres which involve simple process of extraction for example Agave sislana fibres. In most of the cases, the processing of plant material containing raw material for bio-composites as fibres encompass the physical and mild chemical treatments leading to change in original properties of fibres. Therefore, extraction of fibres is also an important factor in considering the evaluation of properties of bio-composites. There are various processes developed and optimized for extraction of fibres and well documented.

The physical treatments of the isolated fibres change the structural and surface properties of the reinforcing fibres without altering the properties and disintegration of fibres. The physical treatments influence the mechanical properties resulting in proper bonding to the matrix and affects interfacial adhesion. The commonly used method for plant-based fibres is corona and cold plasma treatment, however other physical methods are also successfully used for surface activation such as thermotreatment [58, 59]; calandering [60], stretching [61] and hybrid yarns [62]. The corona treatment provides oxidation of the fibres, which changes the surface energy of the fibres and increases the number of aldehyde groups [63, 64, 65]. The corona and cold plasma treatment are called electric discharge methods and mostly used to activate cellulose fibres leading to increase in mechanical strength [63, 64, 66]. Corona treatment

Corona treatment is employed for treatment of fibres to increase the morphological and mechanical properties of lignocellulosic fibres resulting in an improvement of the interfacial compatibility between matrix and fillers. Homogeneity of composite materials, adhesion properties and mechanical properties (tensile strength, Young modulii) increase to a certain level (10–30%) with corona treatment [67, 68]. Recently, Aloe vera fibres [69] were treated with corona discharge during different time intervals and it was observed that rough surface morphology and degradation of fibres occurred due to etching mechanism caused by corona treatment. Plasma treatment

Plasma treatment is an environmentally friendly green electric discharge method for treatment of fibres [70, 71, 72] and provides changes in surface energy, increase of the roughness and micro-cleaning of the treated fibres. The process causes surface crosslinking and can introduce reactive groups. Mostly low plasma treatment is being carried out in presence of gases to alter the surface properties of fibres. The base material is treated under atmospheric plasma glow discharge for various periods of time using helium, helium/nitrogen, and helium/acetylene, argon, oxygen, air etc. gas. The significance lies in the fact that sometimes desired properties obtained in seconds. Intriguingly, changes in surface roughness, tip-surface adhesion, and surface chemistry of the fibres and flexural strength, flexural modulus, and interlaminar shear stress, storage modulus and glass transition temperature increased significantly. The treatment is successfully employed to alter the surface properties of natural fibres used in composites as reinforcing material. The adhesion between sisal fibres and polypropylene matrix [73] increase the interfacial adhesion between flax fibre and matrix polyethylene and unsaturated polyester [74, 75]; improvement in mechanical properties of ramie fibres [76, 77] polypropylene composites, increase in flexural strength and tensile strength of the composite prepared from jute fibre [78, 79, 80, 81] were obtained by employing plasma treatments.

4.2.2 Chemical modification of fibres

Natural fibres possesses high polarity in nature due to the presence of numerous hydroxyl groups on the fibre surface which makes them incompatible with the synthetic hydrophobic matrix resulting in poor interfacial bonding between the cellulosic fibres and the matrix producing bio-composites with lesser physical and mechanical strength. Chemical treatments of fibres are an important step for processing of bio-composites and enhancing the compatibility of fibres to the synthetic matrix. Bi-functional groups are introduced chemically into the fibres leading to activation of hydroxyl groups. The activated hydroxyl groups further react with the synthetic matrix thereby enhancing the interfacial adhesion and compatibility between the fibres and matrix [76, 82]. Reviews and chemical methods have been reported in the past to increase the functional behaviour of hydroxyl groups present in the polysaccharides. These methods have their own limitations for particular industrial aspects and are used for diverse industrial applications [83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94]. Mercerization, benzoylation, acrylation, acrylonitrile grafting, permanganate treatment, peroxide treatment, and isocyanate treatment, bleaching, acetylation, silane and peroxide treatments, etherifications viz. cynoethylation, quaternization, carboxymethylation and various coupling agents are commonly used for lignocellulosic fiber activation [76, 87]. Mercerization of fibres using alkali at different concentration and time period for activation and production of modified fibres with good adhesion properties have been applied for modification of fibres [95, 96]. Transverse strength of flax fibres may be increased sufficiently by the alkaline treatment which led to produce better adhesion properties between flax fibres and epoxy matrix [97]. Introduction of acetyl groups into the cellulosic fibres increases plasticity leading to hydrophobic character and mechanical strength to the reinforcing material [76, 98]. Coupling agents are frequently and successfully used to reduce the interfacial adhesion of fibres to the matrix. Various organosilanes mostly trialkoxysilanes are variably used as coupling agents and the process is referred as Silanization. The reactive alkoxy groups present in the silanes chemically bond with the hydroxyl groups and the formation of polysiloxane structures occurs [58, 99]. Maleic anhydride is another coupling reagent used to increase the interfacial adhesion of biocomposites [100]. Partial removal of lignin on the henequen fibres increases the adsorption of the silane couplings and interaction among the fibre and the matrix [101]. The mechanical properties including tensile, flexural, impact strengths and tensile modulus of the biocomposites were improved several times on Jute fibre polypropylene composites using m-isopropenyl-α-α-dimethylbenzyl-isocyanate (m-TMI) as the coupling agent using grafting process. Further, the tensile modulus of the composites prepared from virgin polypropylene increases manifold [102]. The use of the clay in the bio-composites formulation led to reduced mechanical properties. Intriguingly, techniques such as pre-coated fibres with nanoclay and maleated polyethylene mixture enhance the synergetic effect of the clay and bamboo fiber and further significantly increase the tensile strength, bending modulus and strength of the high density polyethylene bamboo composites [103].

Maleated coupling agents are widely used to strengthen composites containing fillers and fibre reinforcements. The maleated coupling provides efficient interaction of maleic anhydride with the functional surface of fibres and matrix. Agrofibre polypropylene composites were studied by introducing maleated coupler that provides the flexural and tensile strengths by more than 60% with Epolene™ G-3015 increment in comparison to composites without coupler [89]. Maleic anhydride grafted rice husk [104], hemp fibres unsaturated polyester composites coupled with 3-isopropenyl-dimethylbenzyl isocyanate [105], maleic anhydride-grafted polypropylene jute fibre composites [106], coir fiber and m-isopropenyl-α-α-dimethylbenzyl isocyanate grafted polypropylene composites [107] may be implemented in production of superior biocomposites having high mechanical properties and strength.


5. Biocomposites from extractives

5.1 Biocomposites using tannins

Tannins are a group of polyhydroxy phenolic compounds and exhibit good alternatives to synthetic adhesives for green chemistry in developing composites. They are found abundantly in nature. Their functions are to protect the plants against predation and might help in regulating the plant growth. Tannins are heterogeneous in nature and chemically classified into two main groups viz. hydrolysable and condensed tannins. Hydrolysable tannins are small molecular weight (30-3000D) compounds, heterogeneous in nature and hydrolysed by water, acidic or alkaline conditions into smaller water soluble molecules such as gallic acid and ellagic acid (Figure 2a and b) constituting gallotannins and ellagitannins. The gallotannins and ellagitannins comprise of a central sugar unit esterified with several molecules of gallic acid and a dimer of gallic acid as the basic phenolic unit known as ellagic acid respectively. Gallotannins, or commonly tannic acid, is the acknowledged source of the hydrolyzable tannins produced by extraction with water or organic solvents from the galls of certain trees, Quercus infectoria and Rhus chinensis, and pods from Caesalpinia spinosa. The European chestnut tree (Castanea sativa) and the oak species especially Quercus montana also produce hydrolyzable tannins in sufficient amount which are used in leather manufacture. Ellagitannins, a class of hydrolysable tannins produce ellagic acid on hydrolysis under acidic or alkaline conditions. Mostly these are present in angiosperms extractives. Ellagic acid and their derivatives have extensive applications as antioxidants, chelators, technical and biomedical applications. The possible applications viz. antibacterial, antifungal, antiviral, anti-inflammatory, hepato- and cardioprotective, chemopreventive, neuroprotective, anti-diabetic, gastroprotective, antihyperlipidemic, and antidepressant-like activities, among others have gained interest to researchers and reviewed for commercial exploitation [108, 109].

Figure 2.

(a) Gallic acid (b) Ellagic acid (c) 5,7,3,4- tetrahydroxyflavan– 3– ol (d) 5,7,3,4- tetrahydroxyflavan– 3,4– diol (e) ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (f) HBT (1-hydroxybenzotriazole).

Condensed tannins (nonhydrolyzable tannin or proanthocyanidine) the larger polyphenol groups with high molecular weight upto 30000D compounds, form insoluble precipitates in aqueous solution and are the polymerization products of monomeric flavan–3-ol or flavan-3,4-diol precursors (Figure 2c and d) [110] which are joined through stable C-C bonds between C-4 and C-8 and between C-4 and C-6. Tannins are dynamically used in tanning of animal hides in the leather processing industry since 1960s, the beginning of the industry due to interaction and precipitation of the proteins [29], adhesive making (especially wood adhesives), fisheries, beverages manufacturing, animal feed, biosourced foams, wood preservatives, corrosion inhibitors, polyurethane surface coatings, epoxy adhesives, binders for Teflon coatings, as mineral absorption and protein, as iron gall ink production, adhesive production in wood-based industry, anti-corrosive chemical production, uranium recovering from seawater, and removal of mercury and methylmercury from solution. In continuum, tannins are also used as bioactive molecules in nutrition science, 3D printing and biomedical devices [109, 111]. Their presence in natural vegetable material has prompted scientific community for their industrial applications in many different ways. Since historical times their traditional use has allowed their further use after diverse chemical modification for various end use functionalized properties. The main inherent feature of the tannins is due to the presence of phenolic structure similar to synthetic phenols. Mostly the condensed tannins are polymers composed of falvan-3-ol monomers and are mainly extracted from bark and wood for commericial purposes. Structural diversity and functions of varied range of tannins are very well described elsewhere in the literature [112, 113, 114, 115, 116]. Tannins are extracted from plant material by simple methods. Nevertheless, there are various extraction processes were developed to isolate the tannins for diverse applications. However, the extraction process remains a challenge due to their heterogeneity character and compositions. Recently, various extraction processes, technological applications and their pros and cons were reviewed and appeared in leading scientific reports [117, 118, 119]. Due to similar tannin structural properties as that of synthetic phenols, the basis of wood adhesives was started in the middle of 1940s. The world first commercial wood adhesive credit goes to Australia in the 1960s using Acacia mearnsii (black wattle) bark tannins. The successes stories of producing tannin wood adhesive are continued till date with the advancements. The other species used in producing commercial taanins are Pinus radiate (radiate pine) bark [120].

Condensed tannins have been in industrial use for nearly 60 years as replacement for phenolic resins for wood based panels with high resistance against moisture and water as well as for boards with very low subsequent formaldehyde emission. Mimosa tannins, obtained from mimosa bark are usually well appreciated for its functional properties for wood adhesives. A wider industrial usage of tannins suffers from the limited availability of raw materials and high transportation costs. Only South Africa is the only actual producer of mimosa tannins on industrial scale [121]. Tannins react with formaldehyde the main crosslinker, and form hardened and crosslinked structures, similar to synthetic phenolic resins. The methylene bridges are formed between two tannin molecules. These methylene bridges are resistant to environmental factors against hydrolysis due to the strong stability of C-C bonds. Tannin-phenolic resin (tannin wt 40%) and sisal fibres (50 wt%) thermoset adhesives were successfully prepared and met all the required standards viz. Izod impact strength increased significatly. Further, it was also observed that sisal fibres and the tannin–phenolic thermosets have close values of the dispersive component and compatible interaction between the sisal fibres and the tannin–phenolic matrix at the interface [122]. A new source of tannin was also reported as a by product during catechin extraction process from a plant leaves (Uncaria gambier) extract and the tannin-phenol-formaldehyde wood adhesive was successfully prepared which met all the international standards [110]. Further, a new source of fibres, obtained from Pinus roxburghii needles, was also utilized in preparation of composites using phenolic resin adhesives [101]. Since, the early 1990s there are several scientific reports and reviews pertaining to natural fibres and tannin extracts from a natural sources viz. plants, agriculture wastes are available in the literature [122, 123, 124, 125, 126, 127, 128, 129, 130, 131].

5.2 Biocomposites from fibres using enzyme and lignin

The study of enzymatic systems to activate the cellulosic fibres is a green alternative approach to other modifications for preparation of biocomposites and very well scientifically studied in last four decades to improve the surface, chemical, morphological and thermal properties of natural fibres as reinforcement materials. The enzymes offer an inexpensive and ecofriendly attractive option to improve the surfaces of natural fibres for composites.

Laccases (EC1.10.3.1) are multinuclear copper oxidases often called ‘blue’ oxidases that catalyze the oxidation of a wide range of susbstrates including phenols. Fungal laccases (benzenediol:oxygen oxidoreductase, EC1.10.3.2) are obtained from extractives of various fungal strains as an extracellular product. This enzyme is produced extensively in higher plants and fungi. The enzyme is produced by different genera of ascomycetes [132, 133, 134]; deuteromycetes [135, 136] and mainly from basidiomycetes [137]. The production and purification of biotechnological enzymes have been reviewed extensively [138, 139, 140, 141, 142, 143, 144, 145] due to its overwhelming response. The first laccase was obtained from a Japanese lacquer tree (Rhus vernicifera), since then new fungal laccases from Trametes versicolor, Polyporus pinsitus, Rhizocotonia solani and from Ascomycetes Myceliophthora thermophila and Sccytalidium thermophilum etc. were obtained and studied extensively [142, 143, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159].

Commercially, laccases have been used for delignification of wood, production of ethyl alcohol and identification of morphine and codeine etc. among the various applications [142, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170]. Various delignification processes using fungi have been developed by the scientific community successfully. These enzymes were considered to be capable of Cα-Cβ cleavage of the side chain of lignin models and it was suggested that the enzymes participate in lignin degradation [171, 172]. The white rot fungi especially basidiomycetes degrade lignin in natural system more robustly than other organisms. They completely degrade lignin to carbon dioxide and water. The lignin degradation by white rot fungi was extensively studied earlier on Phanerochaete chrysoporium and Sporotrichum pulverulentum [173, 174]. The enzymes lignin peroxidase and manganese peroxidase were the first isolated from the Phanerochaete chrysoporium fungus culture. Lignin peroxidase is capable of ionizing non-phenolic aromatic substrates as an oxidizing peroxidase and produce aryl cation radicals [175, 176], whereas, manganese peroxidase does not degrade the non-phenolic parts of lignin in wood [177]. Further, it was observed that laccases exhibit strong catalytic activity and biotechnological applications in the bleaching of kraft pulp by depolymerising and solubilising lignin in the presence of mediator compounds [168, 178, 179, 180, 181, 182]. These mediator compounds were called laccase mediator systems (LMS). A number of possible mediator compounds have been searched and described for enhancing the activity of enzymes but mostly the ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and HBT (1-hydroxybenzotriazole) (Figure 2e and f), a derivative of benzotriazole have been used as the mediator systems [173, 183]. The oxidation of benzyl alcohols with ABTS2+ (Figures 3 and 4) have been experimentally proved by [182, 183, 184]. The reaction mechanism of lignin degradation with LMS is complex due to the complex nature of LMS and various reactive centres on the lignin molecule. The simple reaction mechanism was postulated by Freudenreich [185] on the non-phenolic lignin model compound, veratryl alcohol and suggested a possible mechanism of delignification of residual lignin. The scientific kinetic studies of veratryl alcohol and benzyl alcohols have also been studied in laccase mediator systems [178, 184, 186, 187].

Figure 3.

Laccase-catalyzed oxidation of veratryl alcohol in the presence of a mediator.

Figure 4.

Oxidation of ABTS by Laccase enzyme (blue colour, λmax753nm).

Laccases have been found to possess catalytic ability not only to degrade lignin and in delignification process for applications in biobleaching process but also observed as their involvement in the in vivo lignin biosynthesis and possibly in lignification woody tissues in higher plants [188, 189, 190, 191, 192, 193, 194, 195, 196]. The approach of oxidation and polymerisation of lignin by the enzymes [161, 162, 197] was advanced to another biotechnological application for compounded materials using wood fibres, lignin and enzyme laccase. The high tensile strength of the woody system or biocomposites is produced by the cellulose fibres and the pressure strength is produced by the lignin matrix which is a cementing material polymerised in situ and held cellulose fibres together resulting in high strength to the natural composites. Cellulose and hemicelluloses (Figure 5a) are macromolecules composed of sugar molecules. Cellulose is composed of only glucose molecules having β-1-4 linkage (Figure 5b), whereas hemicelluloses composed of different sugar monomers aligned in a definite fashion. Cellulose formation by a single glucose molecule in plant cell requires four enzymes and biosynthesis of lignin composed of phenol units utilizes peroxidases and phenoloxidases (Laccases) [198, 199, 200, 201]. The monomeric units of lignin comprised of coumaryl alcohol (H-lignin) present in grasses and agriculture crops, coniferyl alcohol (G-lignin) present in all species, dominant in conifers and syringyl alcohol (S-lignin) present in hard wood species up to 40% (Figure 6).

Figure 5.

(a) Schematic diagram of cellulose and hemicelluloses in cellulose microfibrils arrangement, blue lines: cellulose; red line: hemicelluloses (b) Chemical Structure of cellulose.

Figure 6.

Monomeric units of Lignin (a) Coumaryl alcohol (H-lignin) (b) Coniferyl alcohol (G-lignin) (c) Syringyl alcohol (S-lignin).

The production of composites emanates the same basic principle as the formation of natural wood: wood is processed and fragmented into fibres and small pieces as per need of the required composites. Fibres, isolated from soft or hardwood, fibre bearing species or agriculture wastes, are embedded into a matrix. The matrix or binder may be a urea-formaldehyde, phenol-formaldehyde, resorcinol-formaldehyde, isocynates or in a combination as per requirement of the composites. The postulated theories of delignification of lignin, lignifications of woody tissues, activation of the surface of fibres possessing lignin, by the peroxidases enzymes have been successfully applied to prepare green biocomposites. The use of enzyme for bonding in the wood was first suggested by Nimz [202]. In continuum, several scientific communities have been engaged in producing biocomposites using laccase peroxidase enzyme. Wood fibres are incubated for a certain time with phenoloxidas laccase enzyme and lignin crust on the fibre surface gets activated and oxidized. Activated fibres are compressed by operating standard operating conditions of pressure, temperature etc, and binderless fibre boards may be prepared as per standards [170, 200, 201, 203, 204, 205, 206, 207]. The utilization of peroxidases in production of biocomposites was also applied to fibres in last two decades [208]. Cellulose fiber enzyme composites [207], hemp fibre reinforced composites using enzyme and chelators [209], polypropylene composites using abaca fibre [210], sisal fibre/phenolic resin composites [211], laccase-treated kenaf fibre reinforced composites with polypropylene and maleic anhydride grafted polypropylene as coupling agents [212], rubber wood fibreboards [205], laccase-mediated grafting dodecyl gallate (DG) on the jute fiber composites [213], banana/polypropylene composites [214, 215], coconut fibre composites [216], natural fiber medium density fibreboard [217], jute polypropylene composites [218, 219], flax fibre epoxy Composites [220, 221] were successfully prepared and studied for increasing mechanical properties and interfacial adhesion of the biocomposites. These all studies indicated that enzymes have the potential ability to modify the surface properties of fibres as being utilized in production of biocomposites. The formation of biocomposites has been shown in graphical representation (Figure 7) [163, 165, 200, 201, 203, 204, 222, 223, 224, 225].

Figure 7.

Graphical representation of Biocomposites.


6. Opportunities and future perspectives

In this chapter, we have underlined and discussed the different sources of natural fibres, their properties and the effect of treatments on natural fibres, etc. and further their effective use as reinforcement for polymer composite materials. Natural fibres are lucrative and worthwhile option for biocomposites. However, limitations such as poor thermal stability, moisture absorption and poor compatibility with polymeric matrices are challenges that need to be resolved.

There are a large number of fibres obtained from the natural resources; intriguingly only few of these fibres have been studied in detail for reinforcement of bio-composite materials and other industrial and traditional applications. In the present chapter popular natural fibres have been discussed as reinforced composites materials with combination of synthetic and natural polymers as modified matrix. Among the most popular natural fibres; flax, jute, hemp, sisal, ramie, and kenaf fibres were extensively studied and employed in different applications as reinforced materials. But due to environmental and economic concern other fibres from natural resources such as pine, bagasse, pineapple leaf, coir, oil palm, banana, and agriculture residues are acquiring interest for various value added applications due to their inherent and diverse physical properties. Merits and demerits of the natural fibres and their inherent properties mainly influence the mechanical properties of bio-composites due to interfacial adhesion between the fibre and synthetic matrix.

Variability in natural fibres such as processing conditions, fibre diameter and length, lumen diameter, presence of other compounds such as amount of lignin and hemicelluloses needs to be standardising for the processing of particular fibres. High qualities of fibres are required to increase the potential of fibres as reinforcement materials. Maleated coupling agents are extensively used to enhance the composites strength using fibre as reinforcement material. These agents are used as couplers and bind synthetic matrix and functional surface of fibres and economical in processing. Further, using couplers the strength of fibres increased which lead to increase interfacial adhesion between two dissimilar components. However, maleated couplers illustrate superior performances with polypropylenes, polylactic acid and other polyolefins etc. Further, scientific inputs are required to improve the strength of biocomposites using maleated couplings by incorporating varied fibres.

The diverse ranges of fibres are required to investigate the quantification of residual lignin on the fiber surface and optimization of fiber isolation parameters since during the processing of fibres the amount of residual lignin may be different to the fibres isolated from the same resource. Further, the constituents and structure of lignin is also different in the fibres sourced material viz. soft and hard woods and vegetable crops and agriculture residues. Therefore, proper attention is required to investigate the activation of fibre surface and binder in the biocomposites similar to natural composites. In continuum, diverse range of appropriate laccase mediator systems (LMS) needs attention for biocomposites.

Green composites may be a suitable alternative for petroleum-based synthetic non-environment friendly materials by using enzymes especially ‘laccases’ one of the most ancient and efficient enzymes with promising future applications. The high reduction potential of laccases has led the vast industrial applicability, despite this, laccase potentialities are not fully exploited due to large-scale production, cost and efficiency. Systemetic progress has been made over the last three decades to enhance the utilization of laccase enzyme for various biotechnological applications and it is expected that laccases will be able to compete with other processes of bio-composites. Thus, scientific efforts are need of the hour in order to achieve the economical production of the biocatalyst, development of optimum production conditions like pH, temperature, medium composition and efficient mediator systems and further utilization in lignin activation of fibres.

In view of the above discussion, the following activities may be expedient in bio-composites development from natural resources.

  • Identification and search of new fibres with better inherent properties and compatibility to the synthetic matrices.

  • Agriculture residues consisting of sufficient amount of celluloses.

  • Utilization of tannins and non toxic small aldehydes for making wood adhesives.

  • Search of coupling agents and other bonding agents.

  • Efficient production of laccases and other biotechnological economical enzymes from various fungal resources.

  • Utilization of waste lignin from the paper industry and activation thereof using enzymes for lignin celluloses complexes.

  • Activation of lignin on the fibre surface to act as binder and enzyme mediator systems to form efficient lignin cellulose complexes.

  • Utilization of long and short fibres simultaneously to avoid voids for increasing the compactness and stiffness of bio-composites.

  • Lignin starch/celluloses complexes for biodegradable plastics.

  • Fire and water proof green bio-composites using natural fibres for interior design of automotive, aerospace and applications in construction industry.

  • Utilization of laccases, celluloses, starches and lignin for various food grade composites as an alternative to plastic.



The author is thankful to my parent organization Forest Research Institute, Indian Council of Forestry Research and Education, Dehradun India, where I cultured myself in the Chemistry of Forest Products in the amalgamation of diverse disciplines under one umbrella. My heartfelt thanks are also due to Prof. (L) Aloys Huttermann, Ex-Direktor, Forstbotanisch Institute Der Universitat, Gottingen, Germany for earning the concepts of biocomposites using Enzymes and Prof. Michael J Kennedy Ex-Leader, Forestry Science, Department of Agriculture and Fisheries (Formerly: Department of Primary Industries and Fisheries), Brisbane, Queensland, Australia, for utilization of forest biomass wastes.


Conflicts of interest

The author declares no conflict of interest.


  1. 1. Lovrić, M., Da Re, R., Vidale, E., Prokofieva, I., Wong, J., Pettenella, D., ... & Mavsar, R. (2020). Non-wood forest products in Europe–A quantitative overview. Forest Policy and Economics, 116, 102175
  2. 2. Panayotou, T., & Ashton, P. (1992). Not by timber alone: economics and ecology for sustaining tropical forests. Ch 5, 70-109, Island Press, Washington DC
  3. 3. Talukdar, N. R., Choudhury, P., Barbhuiya, R. A., & Singh, B. (2021). Importance of Non-Timber Forest Products (NTFPs) in rural livelihood: A study in Patharia Hills Reserve Forest, northeast India. Trees, Forests and People, 3, 100042.
  4. 4. Chopra, K. (1993) The value of NTFP: an estimation for tropical deciduous forest in India Econ. Bot., 47, 251-257
  5. 5. Vantomme, P., Markkula, A., & Leslie, R. N. (2002). Non-wood forest products in 15 countries of tropical Asia: a regional and national overview. FAO Regional Office for Asia and the Pacific, ISBN : 974906660X
  6. 6. Weiss, G., Emery, M. R., Corradini, G., & Živojinović, I. (2020). New values of non-wood forest products. Forests, 11(2), 165
  7. 7. Keča, L. J., Keča, N., & Rekola, M. (2013). Value chains of Serbian non-wood forest products. International Forestry Review, 15(3), 315-335
  8. 8. Kurttila, M., Pukkala, T., & Miina, J. (2018). Synergies and trade-offs in the production of NWFPs predicted in boreal forests. Forests, 9(7), 417
  9. 9. Rajendra, K. C. (2018). Contribution of NWFPs in National Economy. Banko Janakari, 28(2), 1-2
  10. 10. Chandramohan, D., & Marimuthu, K. (2011). A review on natural fibres. International Journal of Research and Reviews in Applied Sciences, 8(2), 194-206
  11. 11. Sanjay, M. R., Arpitha, G. R., Naik, L. L., Gopalakrishna, K., & Yogesha, B. (2016). Applications of natural fibres and its composites: An overview. Natural Resources, 7(3), 108-114
  12. 12. Lobovikov, M., (2003). Bamboo and rattan products and trade. J. Bamboo Ratt. 2 (4), 397-406
  13. 13. Anonymous, (2019). Indian State of Forest Report, Bamboo Resources of the Country. Forest Survey of India 16th ed. Chapter 8, Dehradun, Uttarakhand, India
  14. 14. Bystriakova, N., (2003). Bamboo Biodiversity: Information for Planning Conservation and Management in the Asia-pacific Region. UNEP World Conservation Monitoring Centre
  15. 15. Bystriakova, N., Kapos, V., Lysenko, I., (2004). Bamboo biodiversity: Africa, Madagascar and the Americas. UNEP/Earthprint
  16. 16. Sawarkar, A. D., Shrimankar, D. D., Kumar, A., Kumar, A., Singh, E., Singh, L. & Kumar, R. (2020). Commercial clustering of sustainable bamboo species in India. Industrial Crops and Products, 154, 112693.
  17. 17. Seethalakshmi, K. K., Kumar, M. M., Pillai, K. S., & Sarojam, N. (1998). Bamboos of India: A compendium (Vol. 17). Brill
  18. 18. Sharma, Y. M. L. (1980). Bamboos in the Asia Pacific Region. In Bamboo research in Asia: proceedings of a workshop held in Singapore, 28-30 May 1980. IDRC, Ottawa, ON, CA
  19. 19. Borah, E. D., Pathak, K. C., Deka, B., Neog, D., & Borah, K. (2006). Utilization aspects of Bamboo and its market value. The Indian Forester, 423-427
  20. 20. Farrelly, D. (1996). The Book of Bamboo: A Comprehensive Guide to This Remarkable Plant, Its Uses, and Its History. Thames and Hudson Ltd
  21. 21. Hunter, I.R. (2003) Bamboo resources, uses and trade: The future? J. Bamboo Ratt. 2, 319-326
  22. 22. Liese, W., Köhl, M. (2015) Bamboo: the Plant and Its Uses, 299-346, Springer
  23. 23. Amada S, Ichikawa Y, Munekata T, Nagase Y, Shimizu H. (1997) Fiber texture and mechanical graded structure of bamboo. Composites Part B 1997; 28B:13-20
  24. 24. Amada S, Munekata T, Nagase Y, Ichikawa Y, Kirigal A, Yang Z. (1996) The mechanical structures of bamboos in view of functionally gradient and composite materials. J Comput Mater 30(7):800-19
  25. 25. Chuma S, Hirohashi M, Ohgama T, Kasahara Y. (1990) Composite structure and tensile properties of mousou bamboo. J Mater Soc Japan, 39:847-51
  26. 26. Lakkard S.C, Patel J.M. (1981) Mechanical properties of bamboo, a natural composite. J Fibre Sci Tec 14:319-22
  27. 27. Nogata F. & Takahashi H. (1995) Intelligent functionally graded material: bamboo. Compos Eng 5(7):743-51
  28. 28. Rao, K. M. M., & Rao, K. M. (2007). Extraction and tensile properties of natural fibres: Vakka, date and bamboo. Composite structures, 77(3), 288-295
  29. 29. Haslam, E. “In Biochemistry of Plants: A comprehensive Treatise, Vol 7, Secondary Plant Products, Ed. PK Stumpf and EE Conn.” (1981): 527-556
  30. 30. Haslam, E. (1979). Vegetable tannins. In Biochemistry of plant phenolics (pp. 475-523). Springer, Boston, MA
  31. 31. Gupta, V. K. (2019). Application of Natural Dyes, ch6, In Fundamentals of natural dyes and its application on textile substrates. Chemistry and technology of natural and synthetic dyes and pigments. DOI: 10.5772/intechopen.89964
  32. 32. Saxena, S., & Raja, A. S. M. (2014). Natural dyes: sources, chemistry, application and sustainability issues. In Roadmap to sustainable textiles and clothing (pp. 37-80). Springer, Singapore
  33. 33. Gholampour, A., & Ozbakkaloglu, T. (2020). A review of natural fiber composites: Properties, modification and processing techniques, characterization, applications. Journal of Materials Science, 55(3), 829-892.
  34. 34. Rajisha, K. R., Deepa, B., Pothan, L. A., & Thomas, S. (2011). Thermomechanical and spectroscopic characterization of natural fibre composites. Interface Engineering of Natural Fibre Composites for Maximum Performance, 241-274.
  35. 35. Ticoalu, A., Aravinthan, T., & Cardona, F. (2010). A review of current development in natural fiber composites for structural and infrastructure applications. In Proceedings of the southern region engineering conference (SREC 2010) (pp. 113-117). Engineers Australia
  36. 36. Bachtiar D., Sapuan S. M., Zainudin E. S., Khalina A., and Dahlan K. Z. M. (2009) “The tensile properties of single sugar palm (Arenga pinnata) fibre,” presented at the 9th National Symposium on Polymeric Materials (NSPM 2009)
  37. 37. Beckermann G. W. and. Pickering, K. L. (2008) “Engineering and evaluation of hemp fibre reinforced polypropylene composites: Fibre treatment and matrix modification,” Composites: Part A, vol. 39, pp. 979-988
  38. 38. Brouwer, W. “Natural fiber composites in structural components: Alternative applications for sisal? Common Fund for Commodities - Alternative Applications for Sisal and Henequen,” Technical Paper No. 14, Proceedings of a Seminar held by the Food and Agriculture Organization of the UN (FAO) and the Common Fund for Commodities (CFC), 2000
  39. 39. Jose, S., Rajna, S., & Ghosh, P. (2017). Ramie fibre processing and value addition. Asian Journal of Textile, 7(1), 1-9
  40. 40. Jose, S., Salim, R., & Ammayappan, L. (2016). An overview on production, properties, and value addition of pineapple leaf fibers (PALF). Journal of Natural Fibers, 13(3), 362-373
  41. 41. Ketnawa, S., P. Chaiwut, and S. Rawdkuen. (2012). Pineapple wastes: A potential source for bromelain extraction. Food and Bio-Products Processing 90: 385-391. doi:10.1016/j.fbp.2011.12.006
  42. 42. Mohanty K., Misra, M. and Hinrichsen, G. (2000) Biofibres, biodegradable polymers and biocomposites: An overview, Macromolecular Materials and Engineering, vol. 276-277, pp. 1-24
  43. 43. Mwaikambo, L. Y. (2006). Review of the history, properties and application of Plant fibres. African Journal of Science and Technology, Science and Engineering Series 7: 120-133
  44. 44. Siakeng, R.; Jawaid, M.; Ari_n, H.; Sapuan, S.M.; Asim, M.; Saba, N. (2018) Natural fiber reinforced polylactic acid composites: A review. Polym. Compos., 40, 446-463
  45. 45. Sreenivas. H.T., Krishnamurthy, N., & Arpitha, G. R, A. (2020). A comprehensive review on light weight kenaf fiber for automobiles. International Journal of Lightweight Materials and Manufacture, 3(4), 328-337
  46. 46. Ayadi, R., Hanana, M., Mzid, R., Hamrouni, L., Khouja, M. L., & Salhi Hanachi, A. (2017). Hibiscus cannabinus L.–kenaf: a review paper. Journal of Natural Fibers, 14(4), 466-484
  47. 47. Bekele, L. D., Zhang, W., Liu, Y., Duns, G. J., Yu, C., Jin, L., ... & Chen, J. (2017). Preparation and characterization of lemongrass fiber (Cymbopogon species) for reinforcing application in thermoplastic composites. BioResources, 12(3), 5664-5681
  48. 48. Draman, S. F. S., Daik, R., Latif, F. A., & El-Sheikh, S. M. (2014). Characterization and thermal decomposition kinetics of kapok (Ceiba pentandra L.)–based cellulose. BioResources, 9(1), 8-23
  49. 49. Khalil, H. A., Yusra, A. I., Bhat, A. H., & Jawaid, M. (2010). Cell wall ultrastructure, anatomy, lignin distribution, and chemical composition of Malaysian cultivated kenaf fiber. Industrial Crops and Products, 31(1), 113-121
  50. 50. Koohestani, B., Darban, A.K., Mokhtari, P. (2019) Comparison of different natural fiber treatments: a literature review. Int. J. Environ. Sci. Technol. 16, 629-642.
  51. 51. Faruk, O., Bledzki, A. K., Fink, H. P., & Sain, M. (2014). Progress report on natural fiber reinforced composites. Macromolecular Materials and Engineering, 299(1), 9-26.
  52. 52. Jawaid, M. H. P. S., & Khalil, H. A. (2011). Cellulosic/synthetic fibre reinforced polymer hybrid composites: A review. Carbohydrate polymers, 86(1), 1-18..
  53. 53. Ramamoorthy, S. K., Skrifvars, M., & Persson, A. (2015). A review of natural fibres used in biocomposites: plant, animal and regenerated cellulose fibres. Polymer Reviews, 55(1), 107-162.
  54. 54. John, M. J., & Anandjiwala, R. D. (2008). Recent developments in chemical modification and characterization of natural fiber‐reinforced composites. Polymer composites, 29(2), 187-207.
  55. 55. Shalwan and Yousif, B. F. (2013) “In state of art: mechanical and tribological behaviour of polymeric composites based on natural fibres,” Materials & Design, vol. 48, pp. 14-24
  56. 56. Sreekala M. S. and Thomas S. (2003) “Effect of fibre surface modification on water-sorption characteristics of oil palm fibres,” Composites Science and Technology, vol. 63, no. 6, pp. 861-869
  57. 57. Cruz, J., & Fangueiro, R. (2016). Surface modification of natural fibers: a review. Procedia Engineering, 155, 285-288
  58. 58. Bledzki, A. K., & Gassan, J. (1999). Composites reinforced with cellulose based fibres. Progress in polymer science, 24(2), 221-274.
  59. 59. Costa, C. G., Bom, L. F. R. P., Martins, C. R., da Silva, C. F., & de Moraes, M. A. (2020). (Bio) composites of chitin/chitosan with natural fibres. In Handbook of Chitin and Chitosan (pp. 273-298). Elsevier. 10.1016/B978-0-12-817968-0.00009-3
  60. 60. Semsarzadeh M. A., (1986) Fibre matrix interaction in jute-reinforced polyester resin, Polym. Comp., 7(1), 23-25.
  61. 61. Haig Zeronian, S., Kawabata, H., & Alger, K. W. (1990). Factors affecting the tensile properties of nonmercerized and mercerized cotton fibres. Textile Research Journal, 60(3), 179-183
  62. 62. Wulfhorst B, Tetzlaff G, Kaldenhoff R. (1992) Techn Text; 35(3):10-11
  63. 63. Belgacem, M. N., Bataille, P., & Sapieha, S. (1994). Effect of corona modification on the mechanical properties of polypropylene/cellulose composites. Journal of applied polymer science, 53(4), 379-385.
  64. 64. Dong, S., Sapieha, S., & Schreiber, H. P. (1992). Rheological properties of corona modified cellulose/polyethylene composites. Polymer Engineering & Science, 32(22), 1734-1739.
  65. 65. Sakata, I., Morita, M., Tsuruta, N., & Morita, K. (1993). Activation of wood surface by corona treatment to improve adhesive bonding. Journal of Applied Polymer Science, 49(7), 1251-1258
  66. 66. Gao, S., & Zeng, Y. (1993). Surface modification of ultrahigh molecular weight polyethylene fibres by plasma treatment. I. Improving surface adhesion. Journal of applied polymer science, 47(11), 2065-2071.
  67. 67. Ragoubi, M., Bienaimé, D., Molina, S., George, B., & Merlin, A. (2010). Impact of corona treated hemp fibres onto mechanical properties of polypropylene composites made thereof. Industrial Crops and Products, 31(2), 344-349.
  68. 68. Ragoubi, M., George, B., Molina, S., Bienaimé, D., Merlin, A., Hiver, J. M., & Dahoun, A. (2012). Effect of corona discharge treatment on mechanical and thermal properties of composites based on miscanthus fibres and polylactic acid or polypropylene matrix. Composites Part A: Applied Science and Manufacturing, 43(4), 675-685.
  69. 69. Oudrhiri F. H., Merbahi, N., Oushabi, A., Elfadili, M.H., Kammouni, A., Oueldna, N., Effects of corona discharge treatment on surface and mechanical properties of Aloe vera fibres, Materials Today: Proceedings, Volume 24, Part 1, (2020), Pages 46-51, ISSN 2214-7853,
  70. 70. Danmei Sun (2016), Surface Modification of Natural Fibres Using Plasma Treatment In book: Biodegradable Green Composites, Chapter 2: Surface modification of natural fibres using plasma treatment, Publisher: John Wiley & Sons, Editors: Kalia S. DOI: 10.1002/9781118911068
  71. 71. Sobczyk-Guzendaa, A; Szymanowskia, H.; Jakubowskia, W., Błasińskab, A.; Kowalskia J. and Gazicki-Lipmana, M., (2013). Morphology, photocleaning and water wetting properties of cotton fabrics, modified with titanium dioxide coatings synthesized with plasma enhanced chemical vapor deposition technique, Surface and Coatings Technology, 217(25), pp. 51-57
  72. 72. Sun, D. and Stylios, G., (2005). Investigating the plasma modification of natural fibre fabrics – the effect on fabric surface and mechanical properties, Textile Research Journal, 75 (9), pp. 639-645
  73. 73. Mukhopadhyay, S.; Pal, R.; Narula, V.; Mayank, M., (2013). A study of interface behavior in sisal fibre composites - Single fibre pull out test. Indian Journal of Fibre & Textile Research, 38 (1), pp. 87-91
  74. 74. Bozaci E.; Sever K.; Sarikanat M.; Seki Y.; Demir A.; Ozdogan E. and Tavman, I., (2013). Effects of the atmospheric plasma treatments on surface and mechanical properties of flax fiber and adhesion between fiber–matrix for composite materials, Composites: Part B, 45, pp. 565-572
  75. 75. Sarikanat, M., Seki, Y., Sever, K., Bozaci, E., Demir, A., & Ozdogan, E. (2016). The effect of argon and air plasma treatment of flax fiber on mechanical properties of reinforced polyester composite. Journal of Industrial Textiles, 45(6), 1252-1267
  76. 76. Li, X., Tabil, L. G., & Panigrahi, S. (2007). Chemical treatments of natural fiber for use in natural fiber-reinforced composites: a review. Journal of Polymers and the Environment, 15(1), 25-33. 10.1007/s10924-006-0042-3
  77. 77. Zhou, M., Yan, J., Li, Y., Geng, C., He, C., Wang, K., & Fu, Q. (2013). Interfacial strength and mechanical properties of biocomposites based on ramie fibers and poly (butylene succinate). RSC advances, 3(48), 26418-26426
  78. 78. Demir, A., Seki, Y., Bozaci, E., Sarikanat, M., Erden, S., Sever, K., & Ozdogan, E. (2011). Effect of the atmospheric plasma treatment parameters on jute fabric: the effect on mechanical properties of jute fabric/polyester composites. Journal of Applied Polymer Science, 121(2), 634-638
  79. 79. Gibeop, N., Lee, D. W., Prasad, C. V., Toru, F., Kim, B. S., & Song, J. I. (2013). Effect of plasma treatment on mechanical properties of jute fiber/poly (lactic acid) biodegradable composites. Advanced Composite Materials, 22(6), 389-399
  80. 80. Kafi, A. A., Magniez, K., & Fox, B. L. (2011). A surface-property relationship of atmospheric plasma treated jute composites. Composites science and technology, 71(15), 1692-1698.
  81. 81. Kim, B. S.; Nguyen, M. H.; Hwang, B. S. and Lee, S., (2008). Effect of plasma treatment on the mechanical properties of natural fibre/PP composites, in: De Wilde, W.P., Brebbia, C.A. (Eds.), High performance structures and materials IV. WIT transactions on built environment, WIT PRESS, Southampton UK, pp. 159-166
  82. 82. Sathish, S., Karthi, N., Prabhu, L., Gokulkumar, S., Balaji, D., Vigneshkumar, N., ... & Dinesh, V. P. (2021). A review of natural fiber composites: Extraction methods, chemical treatments and applications. Materials Today: Proceedings.
  83. 83. Goyal, P., Kumar, V. and Sharma, P. (2008) Cyanoethylation of Tamarind kernel Powder. Starch/Starke, 60 (1), 41-47
  84. 84. Goyal, P., Kumar, V. and Sharma, Pradeep (2007) Carboxymethylation of Tamarind kernel Powder. Carbohydrate Polymers, 69, 251-255
  85. 85. Gupta, S., Sharma, P. and Soni, P.L. (2004) Carboxymethylation of Cassia occccidentalis seed gum. J. Applied Polymer Science, 94(4) 1606-1611
  86. 86. Gupta, S., Sharma, P. and Soni, P.L. (2005) Chemical modification of Cassia occidentalis seed gum: Carbamoylethylation. Carbohydrate Polymers, 59, 501-506
  87. 87. Kabir, M. M., Wang, H., Lau, K. T., & Cardona, F. (2012). Chemical treatments on plant-based natural fibre reinforced polymer composites: An overview. Composites Part B: Engineering, 43(7), 2883-2892.
  88. 88. Karaki, N., Aljawish, A., Humeau, C., Muniglia, L., & Jasniewski, J. (2016). Enzymatic modification of polysaccharides: Mechanisms, properties, and potential applications: A review. Enzyme and Microbial Technology, 90, 1-18
  89. 89. Keener, T. J., Stuart, R. K., & Brown, T. K. (2004). Maleated coupling agents for natural fibre composites. Composites part A: applied science and manufacturing, 35(3), 357-362.
  90. 90. Li, S., Xiong, Q., Lai, X., Li, X., Wan, M., Zhang, J., ... & Lin, Y. (2016). Molecular modification of polysaccharides and resulting bioactivities. Comprehensive Reviews in Food Science and Food Safety, 15(2), 237-250
  91. 91. Sharma D., Kumar V., Nautiyal R., Sharma P. (2020a) ‘Synthesis and characterization of quaternized Cassia tora gum using Taguchi L’16 approach’, Carbohydrate Polymers, Volume 232, Article115731. (
  92. 92. Sharma D., Kumar V., Sharma P. (2020b) Application, synthesis, and characterization of cationic galactomannan from ruderal species as wet strength additive and flocculating agent” ACS Omega DOI 10.1021/acsomega.0c03408
  93. 93. Sharma P., Gupta S., Soni P.L. and Kumar V. (2020) Ce(IV)-Ion Initiated grafting of 1,3 galactomannan with acrylonitrile. Journal of Macromolecular Science. Part A. Pure and Applied Chemistry, Volume 57(&) 519-530.
  94. 94. Tyagi R., Sharma P., Nautiyal R., Lakhera A. K., and Kumar V. (2020). Synthesis of quaternised guar gum using Taguchi L (16) orthogonal array. Carbohydrate Polymers, 116136. (Volume 237, Article, 116136, ISSN 0144-8617)
  95. 95. Sahu, P., & Gupta, M. K. (2020). A review on the properties of natural fibres and its bio-composites: Effect of alkali treatment. Proceedings of the Institution of Mechanical Engineers, Part L: Journal of Materials: Design and Applications, 234(1), 198-217
  96. 96. Van de Weyenberg, I., Truong, T. C., Vangrimde, B., & Verpoest, I. (2006). Improving the properties of UD flax fibre reinforced composites by applying an alkaline fibre treatment. Composites Part A: Applied Science and Manufacturing, 37(9), 1368-1376.
  97. 97. Pereira, P. H. F., Rosa, M. D. F., Cioffi, M. O. H., Benini, K. C. C. D. C., Milanese, A. C., Voorwald, H. J. C., & Mulinari, D. R. (2015). Vegetal fibres in polymeric composites: a review. Polímeros, 25(1), 9-22.
  98. 98. Hossain, M. K., Karim, M. R., Chowdhury, M. R., Imam, M. A., Hosur, M., Jeelani, S., & Farag, R. (2014). Comparative mechanical and thermal study of chemically treated and untreated single sugarcane fiber bundle. Industrial Crops and Products, 58, 78-90. ttps://
  99. 99. Yanjun Xie, Callum A.S. Hill, Zefang Xiao, Holger Militz, Carsten Mai, (2010) Silane coupling agents used for natural fiber/polymer composites: A review, Composites Part A: Applied Science and Manufacturing, Volume 41, Issue 7,2010, pp 806-819,ISSN 1359-835X,
  100. 100. Valadez-Gonzalez, A., Cervantes-Uc, J. M., Olayo, R., & Herrera-Franco, P. J. (1999). Chemical modification of henequen fibres with an organosilane coupling agent. Composites Part B: Engineering, 30(3), 321-331.
  101. 101. Sinha P., Mathur S., Sharma P., Kumar, V. (2016) Potential of Pine Needles for PLA based composites, Polymer Composites (DOI 10.1002/pc.24074)
  102. 102. Aggarwal, P. K., Raghu, N., Karmarkar, A., & Chuahan, S. (2013). Jute–polypropylene composites using m-TMI-grafted-polypropylene as a coupling agent. Materials & Design, 43, 112-117
  103. 103. Han, G., Lei, Y., Wu, Q., Kojima, Y., & Suzuki, S. (2008). Bamboo–fiber filled high density polyethylene composites: effect of coupling treatment and nanoclay. Journal of Polymers and the Environment, 16(2), 123-130.
  104. 104. Raghu, N., Kale, A., Chauhan, S., & Aggarwal, P. K. (2018). Rice husk reinforced polypropylene composites: Mechanical, morphological and thermal properties. Journal of the Indian Academy of Wood Science, 15(1), 96-104
  105. 105. Liu, W., Chen, T., & Qiu, R. (2014). Effect of fiber modification with 3-isopropenyl-dimethylbenzyl isocyanate (TMI) on the mechanical properties and water absorption of hemp-unsaturated polyester (UPE) composites. Holzforschung, 68(3), 265-271
  106. 106. Singh, H., Singh, J. I. P., Singh, S., Dhawan, V., & Tiwari, S. K. (2018). A brief review of jute fibre and its composites. Materials Today: Proceedings, 5(14), 28427-28437
  107. 107. Nandi, A., Kale, A., Raghu, N., Aggarwal, P. K., & Chauhan, S. S. (2013). Effect of concentration of coupling agent on mechanical properties of coir–polypropylene composite. Journal of the Indian Academy of Wood Science, 10(1), 62-67
  108. 108. Evtyugin, Dmitry D.; Magina, Sandra; Evtuguin, Dmitry V. (2020). “Recent Advances in the Production and Applications of Ellagic Acid and Its Derivatives. A Review” Molecules 25, no. 12: 2745.
  109. 109. Lescano, C. H., de Lima, F. F., Caires, A. R. L., & de Oliveira, I. P. (2019). Polyphenols Present in Campomanesia Genus: Pharmacological and Nutraceutical Approach. In Polyphenols in Plants (pp. 407-420). Academic Press
  110. 110. Mathur S., Sharma P., Shukla K.S., Soni P.L. (2013) Potential of tannins for exterior grade plywood. Forest Products Journal 62 (7/8), 559-565
  111. 111. Younes Shirmohammadli, Davood Efhamisisi, Antonio Pizzi, (2018) Tannins as a sustainable raw material for green chemistry: A review, Industrial Crops and Products, Volume 126,2018, Pages 316-332,ISSN 0926-6690,
  112. 112. De Bruyne, T., Pieters, L., Deelstra, H., & Vlietinck, A. (1999). Condensed vegetable tannins: biodiversity in structure and biological activities. Biochemical Systematics and Ecology, 27(4), 445-459
  113. 113. Ferreira, D., Brandt, E. V., Coetzee, J., & Malan, E. (1999). Condensed tannins. In Fortschritte der Chemie organischer Naturstoffe/Progress in the Chemistry of Organic Natural Products (pp. 21-67). Springer, Vienna
  114. 114. Hemingway, R. W., & Karchesy, J. J. (2012). Chemistry and significance of condensed tannins. Springer Science & Business Media
  115. 115. Schofield, P., Mbugua, D. M., & Pell, A. N. (2001). Analysis of condensed tannins: a review. Animal feed science and technology, 91(1-2), 21-40
  116. 116. Yoshida, T., Hatano, T., & Ito, H. (2005). Chapter 7, High molecular weight plant poplyphenols (tannins): Prospective functions. In Recent advances in phytochemistry (pp. 163-190). Elsevier Inc
  117. 117. Das, A. K., Islam, M. N., Faruk, M. O., Ashaduzzaman, M., & Dungani, R. (2020). Review on tannins: Extraction processes, applications and possibilities. South African Journal of Botany, 135, 58-70. Doi:10.1016/j.sajb.2020.08.008
  118. 118. Fraga-Corral M, García-Oliveira P, Pereira AG, Lourenço-Lopes C, Jimenez-Lopez C, Prieto MA, Simal-Gandara (2020) J. Technological Application of Tannin-Based Extracts. Molecules. Jan 30; 25(3):614. DOI: 10.3390/molecules25030614. PMID: 32019231; PMCID: PMC7037717
  119. 119. Pedro L. de Hoyos-Martínez, Juliette Merle, Jalel Labidi, Fatima Charrier – El Bouhtoury (2019) Tannins extraction: A key point for their valorization and cleaner production, Journal of Cleaner Production, Volume 206, Pages 1138-1155, ISSN 0959-6526,
  120. 120. Yazaki, Y. (2015). Utilization of flavonoid compounds from bark and wood: a review. Natural product communications, 10(30, 5130520. 10.1177/1934578X1501000333
  121. 121. Dunky, M. (2020). Wood Adhesives Based on Natural Resources: A Critical Review Part III. Tannin-and Lignin-Based Adhesives. Reviews of Adhesion and Adhesives, 8(4), 379-525.
  122. 122. Elaine C. Ramires, Elisabete Frollini, (2012) Tannin–phenolic resins: Synthesis, characterization, and application as matrix in biobased composites reinforced with sisal fibres, Composites Part B: Engineering, Volume 43, Issue 7, Pages 2851-2860, ISSN 1359-8368,
  123. 123. Barbosa Jr, V., Ramires, E. C., Razera, I. A. T., & Frollini, E. (2010). Biobased composites from tannin–phenolic polymers reinforced with coir fibers. Industrial Crops and Products, 32(3), 305-312
  124. 124. Nicollin, A., Kueny, R., Toniazzo, L., & Pizzi, A. (2012). High density biocomposite from natural fibers and tannin resin. Journal of Adhesion Science and Technology, 26(10-11), 1537-1545
  125. 125. Nicollin, A., Li, X., Girods, P., Pizzi, A., & Rogaume, Y. (2013). Fast pressing composite using tannin-furfuryl alcohol resin and vegetal fibers reinforcement. Journal of Renewable Materials, 1(4), 311-316
  126. 126. Ramires, E. C., & Frollini, E. (2012). Tannin–phenolic resins: synthesis, characterization, and application as matrix in biobased composites reinforced with sisal fibers. Composites Part B: Engineering, 43(7), 2851-2860
  127. 127. Sauget, A., Nicollin, A., & Pizzi, A. (2013). Fabrication and mechanical analysis of mimosa tannin and commercial flax fibers biocomposites. Journal of adhesion science and technology, 27(20), 2204-2218
  128. 128. Zhu, J., Abhyankar, H., Nassiopoulos, E., & Njuguna, J. (2012). Tannin-based flax fibre reinforced composites for structural applications in vehicles. In IOP Conference Series: Materials Science and Engineering (Vol. 40(1), p. 012030, IOP Publishing
  129. 129. Zhu, J., Njuguna, J., Abhyankar, H., Zhu, H., Perreux, D., Thiebaud, F., ... & Nicollin, A. (2013). Effect of fibre configurations on mechanical properties of flax/tannin composites. Industrial crops and products, 50, 68-76
  130. 130. Zhu, J., Zhu, H., Immonen, K., Brighton, J., & Abhyankar, H. (2015). Improving mechanical properties of novel flax/tannin composites through different chemical treatments. Industrial Crops and Products, 67, 346-354
  131. 131. Zhu, X., Hou, X., Ma, B., Xu, H., & Yang, Y. (2019). Chitosan/gallnut tannins composite fiber with improved tensile, antibacterial and fluorescence properties. Carbohydrate polymers, 226, 115311
  132. 132. Durrens, P. (1981). The phenoloxidases of the ascomycete Podospora anserina: the three forms of the major laccase activity. Archives of Microbiology, 130(2), 121-124
  133. 133. Froehner, S. C., & Eriksson, K. E. (1974). Induction of Neurospora crassa laccase with protein synthesis inhibitors. Journal of bacteriology, 120(1), 450-457
  134. 134. Kurtz, M. B., & Champ, S. P. (1982). Purification and characterization of the conidial laccase of Aspergillus nidulans. Journal of bacteriology, 151(3), 1338-1345
  135. 135. Dubernet, M., Ribereau-Gayon, P., Lerner, H. R., Harel, E., & Mayer, A. M. (1977). Purification and properties of laccase from Botrytis cinerea. Phytochemistry, 16(2), 191-193
  136. 136. Gigi, O., Marbach, I., & Mayer, A. M. (1981). Properties of gallic acid-induced extracellular laccase of Botrytis cinerea. Phytochemistry, 20(6), 1211-1213
  137. 137. Scháněl, L. (1967). A new polyphenoloxidase test for distinguishing between wood-rotting fungi. Biologia Plantarum, 9(1), 41-48
  138. 138. Chefetz, B., Chen, Y., & Hadar, Y. (1998). Purification and characterization of laccase fromChaetomium thermophilium and its role in humification. Applied and Environmental Microbiology, 64(9), 3175-3179
  139. 139. Mayer, A. M. (1986). Polyphenol oxidases in plants-recent progress. Phytochemistry, 26(1), 11-20
  140. 140. Reinhammar, B. & Malmstrom, B. G. (1981). Metal Ions in Biology: Copper Proteins, 3. Wiley, New York, 109-149
  141. 141. Reinhammar, B. (1970). Purification and properties of laccase and stellacyanin from Rhus vernicifera. Biochimica et Biophysica Acta (BBA)-Bioenergetics, 205(1), 35-47
  142. 142. Senthivelan, T., Kanagaraj, J., & Panda, R. C. (2016). Recent trends in fungal laccase for various industrial applications: an eco-friendly approach-a review. Biotechnology and Bioprocess Engineering, 21(1), 19-38
  143. 143. Shekher, R., Sehgal, S., Kamthania, M., & Kumar, A. (2011). Laccase: microbial sources, production, purification, and potential biotechnological applications. Enzyme Research, 1-11
  144. 144. Yaropolov, A. I., Skorobogat’Ko, O. V., Vartanov, S. S., & Varfolomeyev, S. D. (1994). Laccase. Applied Biochemistry and Biotechnology, 49(3), 257-280.
  145. 145. Bertrand, B., Martínez-Morales, F., & Trejo-Hernandez, M. R. (2013). Fungal laccases: induction and production. Revista mexicana de ingeniería química, 12(3), 473-488
  146. 146. Ali, W. B., Chaduli, D., Navarro, D., Lechat, C., Turbé-Doan, A., Bertrand, E., ... & Mechichi, T. (2020). Screening of five marine-derived fungal strains for their potential to produce oxidases with laccase activities suitable for biotechnological applications. BMC biotechnology, 20(1), 1-13
  147. 147. Debnath, R., Mistry, P., Roy, P., Roy, B., & Saha, T. (2021). Partial purification and characterization of a thermophilic and alkali-stable laccase of Phoma herbarum isolate KU4 with dye-decolorization efficiency. Preparative Biochemistry & Biotechnology, 1-18
  148. 148. Desai, S. S., Tennali, G. B., Channur, N., Anup, A. C., Deshpande, G., & Murtuza, B. A. (2011). Isolation of laccase producing fungi and partial characterization of laccase. Biotechnol. Bioinf. Bioeng, 1(4), 543-549
  149. 149. Devasia, S., & Nair, A. J. (2016). Screening of potent laccase producing organisms based on the oxidation pattern of different phenolic substrates. Int J Curr Microbiol App Sci, 5(5), 127-137
  150. 150. Forootanfar, H., & Faramarzi, M. A. (2015). Insights into laccase producing organisms, fermentation states, purification strategies, and biotechnological applications. Biotechnology progress, 31(6), 1443-1463
  151. 151. Khalid, N., Asgher, M., & Qamar, S. A. (2020). Evolving trend of Boletus versicolor IBL-04 by chemical mutagenesis to overproduce laccase: Process optimization, 3-step purification, and characterization. Industrial Crops and Products, 155, 112771
  152. 152. Kiiskinen, L. L., Rättö, M., & Kruus, K. (2004). Screening for novel laccase‐producing microbes. Journal of applied microbiology, 97(3), 640-646
  153. 153. Luterek, J., Gianfreda, L., Wojtas-Wasilewska, M., Rogalski, J., Jaszek, M., Malarczyk, E., ... & Leonowicz, A. (1997). Screening of the wood-rotting fungi for laccase production: induction by ferulic acid, partial purification, and immobilization of laccase from the high laccase-producing strain, Cerrena unicolor. Acta Microbiologica Polonica, 46(3)
  154. 154. Nikolaivits, E., Siaperas, R., Agrafiotis, A., Ouazzani, J., Magoulas, A., Gioti, Α., & Topakas, E. (2021). Functional and transcriptomic investigation of laccase activity in the presence of PCB29 identifies two novel enzymes and the multicopper oxidase repertoire of a marine-derived fungus. Science of The Total Environment, 145818
  155. 155. Revankar, M. S., & Lele, S. S. (2006). Enhanced production of laccase using a new isolate of white rot fungus WR-1. Process Biochemistry, 41(3), 581-588
  156. 156. Tapia-Tussell, R., Pérez-Brito, D., Rojas-Herrera, R., Cortes-Velazquez, A., Rivera-Muñoz, G., & Solis-Pereira, S. (2011). New laccase-producing fungi isolates with biotechnological potential in dye decolorization. African Journal of Biotechnology, 10(50), 10134-10142
  157. 157. Wang, F., Xu, L., Zhao, L., Ding, Z., Ma, H., & Terry, N. (2019). Fungal laccase production from lignocellulosic agricultural wastes by solid-state fermentation: a review. Microorganisms, 7(12), 665
  158. 158. Xu, F., Shin, W., Brown, S. H., Wahleithner, J. A., Sundaram, U. M., & Solomon, E. I. (1996). A study of a series of recombinant fungal laccases and bilirubin oxidase that exhibit significant differences in redox potential, substrate specificity, and stability. Biochimica et Biophysica Acta (BBA)-Protein Structure and Molecular Enzymology, 1292(2), 303-311.
  159. 159. Yang, X., Wu, Y., Zhang, Y., Yang, E., Qu, Y., Xu, H., ... & Yan, J. (2020). A thermo-active laccase isoenzyme from Trametes trogii and its potential for dye decolorization at high temperature. Frontiers in microbiology, 11, 241
  160. 160. Bauer, C. G., Kühn, A., Gajovic, N., Skorobogatko, O., Holt, P. J., Bruce, N. C., ... & Scheller, F. W. (1999). New enzyme sensors for morphine and codeine based on morphine dehydrogenase and laccase. Fresenius’ journal of analytical chemistry, 364(1), 179-183. DOI: 10.1007/s002160051320
  161. 161. Haars A. & Hüttermann A (1980). Macromolecular mechanism of lignin degradation by Fomes annosus. Naturwissenschaften 67, 39-40
  162. 162. Haars, A. & Hüttermann, A. (1980). Function of laccase in the white-rot fungus Fomes annosus. Archives of microbiology, 125(3), 233-237
  163. 163. Mayer, A. M., & Staples, R. C. (2002). Laccase: new functions for an old enzyme. Phytochemistry, 60(6), 551-565.
  164. 164. Moreno, A. D., Ibarra, D., Fernández, J. L., & Ballesteros, M. (2012). Different laccase detoxification strategies for ethanol production from lignocellulosic biomass by the thermotolerant yeast Kluyveromyces marxianus CECT 10875. Bioresource Technology, 106, 101-109.
  165. 165. Patel, N., Shahane, S., Majumdar, R., & Mishra, U. (2019). Mode of action, properties, production, and application of laccase: a review. Recent patents on biotechnology, 13(1), 19-32.
  166. 166. Pezzella, C., Guarino, L., & Piscitelli, A. (2015). How to enjoy laccases. Cellular and Molecular Life Sciences, 72(5), 923-940
  167. 167. Plácido, J., & Capareda, S. (2015). Ligninolytic enzymes: a biotechnological alternative for bioethanol production. Bioresources and Bioprocessing, 2(1), 1-12.
  168. 168. Sealey, J. E., Ragauskas, A. J., & Elder, T. J. (1999). Investigations into laccase-mediator delignification of kraft pulps. Holzforschung, 53, 498-502
  169. 169. Tanaka, H., Itakura, S., & Enoki, A. (1999). Hydroxyl radical generation by an extracellular low-molecular-weight substance and phenol oxidase activity during wood degradation by the white-rot basidiomycete Trametes versicolor. Journal of Biotechnology, 75(1), 57-70
  170. 170. Widsten, P., & Kandelbauer, A. (2008). Laccase applications in the forest products industry: a review. Enzyme and microbial technology, 42(4), 293-307
  171. 171. Eriksson, K. E. L., Blanchette, R. A., & Ander, P. (1990). Biodegradation of lignin. In Microbial and enzymatic degradation of wood and wood components (pp. 225-333). Springer, Berlin, Heidelberg
  172. 172. Kawai, S., Umezawa, T., & Higuchi, T. (1988). Degradation mechanisms of phenolic β-1 lignin substructure model compounds by laccase of Coriolus versicolor. Archives of biochemistry and biophysics, 262(1), 99-110.
  173. 173. Call, H. P., & Mücke, I. (1997). History, overview and applications of mediated lignolytic systems, especially laccase-mediator-systems (Lignozym®-process). Journal of biotechnology, 53(2-3), 163-202.
  174. 174. Cullen, D. (1997). Recent advances on the molecular genetics of ligninolytic fungi. Journal of Biotechnology, 53(2-3), 273-289
  175. 175. Kersten, P. J., & Kirk, T. K. (1987). Involvement of a new enzyme, glyoxal oxidase, in extracellular H2O2 production by Phanerochaete chrysosporium. Journal of bacteriology, 169(5), 2195-2201
  176. 176. Kersten, P. J., Tien, M., Kalyanaraman, B., & Kirk, T. K. (1985). The ligninase of Phanerochaete chrysosporium generates cation radicals from methoxybenzenes. Journal of Biological Chemistry, 260(5), 2609-2612
  177. 177. Hammel, K. E., Jensen Jr, K. A., Mozuch, M. D., Landucci, L. L., Tien, M., & Pease, E. A. (1993). Ligninolysis by a purified lignin peroxidase. Journal of Biological Chemistry, 268(17), 12274-12281
  178. 178. Balakshin, M., Capanema, E., Chen, C. L., Gratzl, J., Kirkman, A., & Gracz, H. (2001a). Biobleaching of pulp with dioxygen in the laccase-mediator system—reaction mechanisms for degradation of residual lignin. Journal of Molecular Catalysis B: Enzymatic, 13(1-3), 1-16.
  179. 179. Balakshin, M., Chen, C. L., Gratzl, J. S., Kirkman, A. G., & Jakob, H. (2001b). Biobleaching of pulp with dioxygen in laccase-mediator system—effect of variables on the reaction kinetics. Journal of Molecular Catalysis B: Enzymatic, 16(3-4), 205-215
  180. 180. Balakshin, M., Chen, C. L., Gratzl, J. S., Kirkman, A. G., Jakob, H., & Degussa, A. G. (1999). Biobleaching of pulp with dioxygen in the laccase-mediator system. Holzforschung, 54, 390-396
  181. 181. Bourbonnais, R., & Paice, M. G. (1992). Demethylation and delignification of kraft pulp by Trametes versicolor laccase in the presence of 2, 2′-azinobis-(3-ethylbenzthiazoline-6-sulphonate). Applied Microbiology and biotechnology, 36(6), 823-827.
  182. 182. Bourbonnaus, R. (1996). Enzymatic delignification of kraft pulp using laccase and a mediator. TAPPI J., 79, 199-204
  183. 183. Majcherczyk, A., Johannes, C., & Hüttermann, A. (1999). Oxidation of aromatic alcohols by laccase from Trametes versicolor mediated by the 2, 2′-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) cation radical and dication. Applied microbiology and biotechnology, 51(2), 267-276.
  184. 184. Balakshin, M. Y., Chen, C. L., Gratzl, J. S., Kirkman, A. G., Jakob, H., & Degussa, A. G. (2000). Kinetic studies on oxidation of veratryl alcohol by laccase-mediator system. Holzforschung, 54(2), 165-170
  185. 185. Freudenreich, J., Amann, M., Fritz-Langhals, E., & Stohrer, J. (1998). Understanding the Lignozym-process. In International Pulp Bleaching Conference Vol.1, 71-76
  186. 186. Munk, L., Sitarz, A. K., Kalyani, D. C., Mikkelsen, J. D., & Meyer, A. S. (2015). Can laccases catalyze bond cleavage in lignin? Biotechnology advances, 33(1), 13-24.
  187. 187. Potthast, A., Rosenau, T., & Fischer, K. (2001). Oxidation of benzyl alcohols by the laccase-mediator system (LMS) a comprehensive kinetic description. Holzforschung 55, 47-56
  188. 188. Berthet, S., Thevenin, J., Baratiny, D., Demont-Caulet, N., Debeaujon, I., Bidzinski, P., & Jouanin, L. (2012). Role of plant laccases in lignin polymerization. Advances in Botanical Research, 61, 145-172.
  189. 189. De Gonzalo, G., Colpa, D. I., Habib, M. H., & Fraaije, M. W. (2016). Bacterial enzymes involved in lignin degradation. Journal of biotechnology, 236, 110-119.
  190. 190. Dean, J. F., & Eriksson, K. E. L. (1994). Laccase and the deposition of lignin in vascular plants. Holzforschung, 48, 21-21
  191. 191. Felby, C., Pedersen, L. S., & Nielsen, B. R. (1997). Enhanced auto adhesion of wood fibres using phenol oxidases. Holzforschung-International Journal of the Biology, Chemistry, Physics and Technology of Wood, 51(3), 281-286
  192. 192. Gavnholt, B., & Larsen, K. (2002). Molecular biology of plant laccases in relation to lignin formation. Physiologia plantarum, 116(3), 273-280.
  193. 193. Ikeda, R., Sugihara, J., Uyama, H., & Kobayashi, S. (1996). Enzymatic oxidative polymerization of 2, 6-dimethylphenol. Macromolecules, 29(27), 8702-8705.
  194. 194. Mattinen, M. L., Suortti, T., Gosselink, R. J. A., Argyropoulos, D. S., Evtuguin, D., Suurnäkki, A., & Tamminen, T. (2008). Polymerization of different lignins by laccase. BioResources, 3(2), 549-565
  195. 195. Tobimatsu, Y., & Schuetz, M. (2019). Lignin polymerization: how do plants manage the chemistry so well? Current Opinion in Biotechnology, 56, 75-81.
  196. 196. Zhao, Q., Nakashima, J., Chen, F., Yin, Y., Fu, C., Yun, J., ... & Dixon, R. A. (2013). Laccase is necessary and nonredundant with peroxidase for lignin polymerization during vascular development in Arabidopsis. The Plant Cell, 25(10), 3976-3987.
  197. 197. Milstein, O., Hüttermann, A., Fründ, R., & Lüdemann, H. D. (1994). Enzymatic co-polymerization of lignin with low-molecular mass compounds. Applied Microbiology and Biotechnology, 40(5), 760-767
  198. 198. Higuchi, T. (2012a). Biochemistry and molecular biology of wood. Springer Science & Business Media
  199. 199. Higuchi, T. (Ed.). (2012b). Biosynthesis and biodegradation of wood components. Elsevier
  200. 200. Kharazipour, A., Bergmann, K., Nonninger, K., & Hüttermann, A. (1998). Properties of fibre boards obtained by activation of the middle lamella lignin of wood fibres with peroxidase and H2O2 before conventional pressing. Journal of adhesion science and technology, 12(10), 1045-1053
  201. 201. Kharazipour, A., Mai, C., & Hüttermann, A. (1998). Polyphenoles for compounded materials. Polymer degradation and stability, 59(1-3), 237-243
  202. 202. Nimz, H., Razvi, A., Marquharab, I., & Clad, D. (1972). Bindemittel bzw Klebemittel zur Herstellung von Holzwerkstoffen sowie zur Verklebung von Werkstoffen verschiedener Art. German patent DOS, 2221353
  203. 203. Hüttermann, A., Mai, C., & Kharazipour, A. (2001). Modification of lignin for the production of new compounded materials. Applied microbiology and biotechnology, 55(4), 387-394
  204. 204. Kharazipour, A., Hüttermann, A., & Luedemann, H. D. (1997). Enzymatic activation of wood fibres as a means for the production of wood composites. Journal of Adhesion Science and Technology, 11(3), 419-427
  205. 205. Nasir, M., Gupta, A., Beg, M. D. H., Chua, G. K., Jawaid, M., Kumar, A., & Khan, T. A. (2013). Fabricating eco-friendly binderless fiberboard from laccase-treated rubber wood fiber. BioResources, 8(3), 3599-3608
  206. 206. Sharma P. & Huttermann A. (2001) Wood composites by Enzymatic methods. Report submitted to Forstbotanisches Institut Der Universitat Gottingen Busgenweg-237077 Gottingen Germany
  207. 207. Xing, Q., Eadula, S. R., & Lvov, Y. M. (2007). Cellulose fiber enzyme composites fabricated through layer-by-layer nanoassembly. Biomacromolecules, 8(6), 1987-1991
  208. 208. Nasir, M., Hashim, R., Sulaiman, O., Nordin, N. A., Lamaming, J., & Asim, M. (2015). Laccase, an emerging tool to fabricate green composites: a review. BioResources, 10(3), 6262-6284
  209. 209. Li, Y., & Pickering, K. L. (2008). Hemp fibre reinforced composites using chelator and enzyme treatments. Composites science and technology, 68(15-16), 3293-3298.
  210. 210. Bledzki, A. K., Mamun, A. A., Jaszkiewicz, A., & Erdmann, K. (2010). Polypropylene composites with enzyme modified abaca fibre. Composites Science and Technology, 70(5), 854-860
  211. 211. Peng, X., Zhong, L., Ren, J., & Sun, R. (2010). Laccase and alkali treatments of cellulose fibre: Surface lignin and its influences on fibre surface properties and interfacial behaviour of sisal fibre/phenolic resin composites. Composites Part A: Applied Science and Manufacturing, 41(12), 1848-1856
  212. 212. Islam, M. R., Beg, M. D., & Gupta, A. (2013). Characterization of laccase-treated kenaf fibre reinforced recycled polypropylene composites. BioResources, 8(3), 3753-3770
  213. 213. Dong, A., Yu, Y., Yuan, J., Wang, Q., & Fan, X. (2014). Hydrophobic modification of jute fiber used for composite reinforcement via laccase-mediated grafting. Applied surface science, 301, 418-427
  214. 214. Vishnu Vardhini, K. J., & Murugan, R. (2017). Effect of laccase and xylanase enzyme treatment on chemical and mechanical properties of banana fiber. Journal of Natural Fibres, 14(2), 217-227.
  215. 215. Vishnu Vardhini, K. J., Murugan, R., & Surjit, R. (2018). Effect of alkali and enzymatic treatments of banana fibre on properties of banana/polypropylene composites. Journal of Industrial Textiles, 47(7), 1849-1864.
  216. 216. Thakur, K., Kalia, S., Kaith, B. S., Pathania, D., & Kumar, A. (2015). Surface functionalization of coconut fibres by enzymatic biografting of syringaldehyde for the development of biocomposites. RSC advances, 5(94), 76844-76851.
  217. 217. Nasir, M., Hashim, R., Sulaiman, O., Gupta, A., Khan, T. A., Jawaid, M., & Asim, M. (2017). Natural fiber improvement by laccase; optimization, characterization and application in medium density fiberboard. Journal of Natural Fibres, 14(3), 379-389
  218. 218. Dong, A., Li, F., Fan, X., Wang, Q., Yu, Y., Wang, P., & Cavaco-Paulo, A. (2018). Enzymatic modification of jute fabrics for enhancing the reinforcement in jute/PP composites. Journal of Thermoplastic Composite Materials, 31(4), 483-499
  219. 219. Singh, A. & Palsule, S. (2016). Jute fiber reinforced chemically functionalized polypropylene self-compatibilizing composites by Palsule process. Journal of Composite Materials, 50(9), 1199-1212
  220. 220. Brodowsky, H. M., Hennig, A., Müller, M. T., Werner, A., Zhandarov, S., & Gohs, U. (2020). Laccase-Enzyme Treated Flax Fibre for Use in Natural Fibre Epoxy Composites. Materials, 13(20), 4529
  221. 221. Liu, M., Baum, A., Odermatt, J., Berger, J., Yu, L., Zeuner, B., Thygesen, A., Holck, J. and (2017). Oxidation of lignin in hemp fibres by laccase: Effects on mechanical properties of hemp fibres and unidirectional fibre/epoxy composites. Composites Part A: Applied Science and Manufacturing, 95, 377-387
  222. 222. Acero, E. H., Kudanga, T., Ortner, A., Kaluzna, I., De Wildeman, S., Nyanhongo, G. S., & Guebitz, G. M. (2014). Laccase functionalization of flax and coconut fibers. Polymers, 6(6), 1676-1684.
  223. 223. Kalia, S., Thakur, K., Kumar, A., & Celli, A. (2014). Laccase-assisted surface functionalization of lignocellulosics. Journal of Molecular Catalysis B: Enzymatic, 102, 48-58.
  224. 224. Mayer, A. M. (2006). Polyphenol oxidases in plants and fungi: going places? A review. Phytochemistry, 67(21), 2318-2331. 10.1016/j.phytochem. 2006.08.006
  225. 225. Nasir M., Asim M., Singh K. (2021) Fiberboard Manufacturing from Laccase Activated Lignin Based Bioadhesive. In: Jawaid M., Khan T.A., Nasir M., Asim M. (eds) Eco-Friendly Adhesives for Wood and Natural Fiber Composites. Composites Science and Technology. Springer, Singapore.

Written By

Pradeep Sharma

Submitted: 19 March 2021 Reviewed: 21 April 2021 Published: 29 May 2021