Open access peer-reviewed chapter

Natural Products for Salmonellosis: Last Decade Research

By Nancy Jannet Ruiz-Pérez, Jaime Sánchez-Navarrete and Julia D. Toscano-Garibay

Submitted: November 2nd 2020Reviewed: January 25th 2021Published: March 1st 2021

DOI: 10.5772/intechopen.96207

Downloaded: 151

Abstract

Salmonellosis is a disease of great relevance in terms of public health given the economic and social impact that causes both in developing and highly industrialized countries. Due to its transmission mechanism, it affects hundreds or thousands of people every year and is considered an acute disease of worldwide distribution. Causative agent of salmonellosis is salmonella specie which are small gram-negative bacilli and facultative intracellular pathogen of the Enterobacteriaceae family. Multidrug resistance is reported more frequently in strains of salmonella, raising the necessity of new strategies to combat its spread and to treat the disease. Natural products (NPs) derived from traditional medicine knowledge have become an important resource to this end. In this chapter, we present a summary of information published from 2010 to 2020, as a sample of the potentiality of NPs as agents for Salmonellosis. This search was not exhaustive, rather, we aim to obtain a random sample of information using the simplest terms on the matter of natural products for salmonellosis, hopefully, as a reference source for interested researchers.

Keywords

  • salmonella
  • antibacterial activity
  • natural products
  • anti-salmonella
  • Salmonellosis

1. Introduction

Salmonellosis is a disease of great relevance in terms of public health given the economic and social impact that causes both in developing and highly industrialized countries. Due to its transmission mechanism, it affects hundreds or thousands of people every year and is considered an acute disease of worldwide distribution [1] with variations in the frequency of serotypes from one country to another [2], being notably more frequent in areas that have not reached adequate sanitation and hygiene conditions or that do not have enough resources and public health infrastructure. There is no distinction in the occurrence of salmonellosis by sex, age, or social and economic status with high incidence at the extremes of life, being the most vulnerable groups, children under 5yo, adults over 60 years of age and immunocompromised individuals [3, 4]. On the other hand, it is also a seasonal disease, so incidence is higher on periods of increased environmental temperature like spring and summer, showing a decrease in autumn and winter [5].

The raise in salmonellosis at any part of the world is of maximum relevance. For example, an incidence of 0.78–3.8 million cases per year has been estimated in the United States. Natural reservoir is made up of domestic animals (dogs and cats), wild animals (reptiles such as iguanas and turtles) as well as humans (carriers, convalescent). Transmission is through food (with or without manufacture) and water contaminated with human or animal feces and from individual to individual. Salmonellosis presents as sporadic cases or as outbreaks with variable affectations. Incidence rate is dose-dependent in function of the disseminated serotype and is determined by incubation period, symptoms and severity.

Causative agent of salmonellosis are salmonellaspecies, numerous disease outbreaks are related to the consumption of eggs, chicken meat and other raw products (mainly dairy). For instance, in an outbreak of enteric salmonellosis serotype Typhimurium (n = 99) induced by consumption of roast porcine meat in an institution for the mentally ill in Konagua it was shown that the incubation period was between 10–12 hours and that the supply of antibiotics prolonged excreta periods. Salmonellosis due to Salmonella entericaserotype Enteritidis was detected in an interstate outbreak in the United States in the early 90’s, produced by the consumption of ice cream (224,000 cases) and in Canada due to the consumption of commercial packaged cheese (800 cases). Salmonella Javiana (n = 66) has been reported to produce outbreaks as in Boston due to the consumption of chicken sandwiches [6].

Advertisement

2. Salmonella

Salmonellabelongs to the Enterobacteriaceae family, which are small gram-negative bacilli varying in sizes ranging in average from 2–3 μm in length and 0.4–0.6 μm in width. These bacilli do not form spores and possess peritrichous flagella hence are mobile microorganisms, although some genera, such as Klebsiella and Shigella, are lacking on these organelles and so on mobility. Traditional grouping classification is carried out using primary biochemical characteristics that allows a further sorting into subgroups based on antigenic structure determinants or using bacteriophage reactions. Currently, with the advances in molecular biology, the differentiation of groups and subgroups can be made using PCR technique for identification, diagnostic and epidemiological purposes.

Regarding its metabolic characteristics, salmonellagrows in simple synthetic media and can use unique carbon sources, such as glucose in a fermentative way with the subsequent formation of acids and/or gases, reducing nitrates and nitrites, rendering oxidase negative reaction. Salmonellaalso tests positive for methyl red, hydrogen sulfide, indole-ornithine motility (MIO medium), lysine decarboxylase, arginine dihydrolase, ornithine decarboxylase, gas from glucose, and fermentation of numerous carbohydrates such as rhamnose, arabinose, mannitol, etc.

Most enteric microorganisms are resistant to inhibition by the action of certain bacteriostatic dyes, the selective media containing these compounds facilitate considerably isolation from fecal samples, salmonellais less sensitive than coliform microorganisms against citrate inhibition action; for instance, SS (Salmonella-Shigella) agar containing both citrate and bile salts is therefore used as a selective medium for the culture of pathogenic species [7, 8].

Advertisement

3. Classification

Although controversial and evolving, there is a salmonellanomenclature used by the Centers for Disease Control and Prevention (CDC) and recommended by the Collaborating Center of the World Health Organization (WHO), which according to the differences in their 16S rRNA sequence analysis classifies this genus into two species, Salmonella entericaand Salmonella bongori. S. entericacan also be further classified into six subspecies mainly found in mammals and is responsible for 99% of infections in humans and warm-blooded animals. On the other hand, S. bongoriis predominantly environmental and on cold-blooded animals [9].

3.1 Classification according to Kauffmann-White

Since decades ago, classification of salmonellafinalizing at the species level are based on its antigenic structure. Although certain strains that have the same antigenic activity could present different metabolic reactions (biotype variants or serotypes), this sorting method is generally accepted and is actively in use.

Surface antigen studies are based on H, O, K and Vi antigens. H is denominated surface or flagellar antigen and participates in host immune response, O (aka somatic antigen) is a lipopolysaccharide located in the cell membrane, K is a capsular antigen and Vi antigen is a subtype of K antigen associated to virulence [9] and the obtention of antisera containing antibodies against all these fractions allows the identification of salmonellaspecies. More than 2,500 serotypes have been identified related to the H, O, K and Vi antigens [10] as a result of the numerous absorption tests and cross-reactions studies carried out in Denmark and England by Kauffman and White. Currently, large centers in Copenhagen, London, and Atlanta have the necessary collections of specific antisera for salmonellas typing. In most testing and diagnostic laboratories, salmonellastrains are identified and classified by their fermentative characteristics and agglutination reactions using group-specific antisera.

In Mexico, for example, a study for the classification and identification of salmonellaserotypes at public and private health centers and hospitals analyzed 24,394 salmonellastrains isolated from different sources, 15,843 (64.9%) of human origin and 8,551 (35.1%) non-human demonstrating the usefulness of Kauffmann-White scheme and using antisera produced at the National Institute of Diagnosis and Reference (INDRE) in accordance with the Center for Disease Control and Prevention, Atlanta (GA), showing that most frequent serotypes both in human and non-human samples were S.Typhimurium, S.Enteritidis, S.Derby, S.Agona and S.Anatum. From the epidemiological point of view, it is interesting to identify which are the circulating and emerging serotypes to implement prevention strategies [8].

Advertisement

4. Pathogenicity

Salmonellaspp. is a highly pathogenic microorganism that presents different pathogenicity mechanisms including adherence, invasiveness, colonization and growth, toxicity and tissue damage [11]. It is a facultative intracellular pathogen causing moderate to severe infections, or even compromising systemic infections risking patients’ lives, depending on the serotype, virulence, inoculum and immunological state of involved host, and all of this using only a mixture of toxins and other virulence factors.

Clinical manifestations in humans include enteric fevers, acute gastroenteritis and septicemia in extreme cases. Prototypical enteric fevers are caused by SalmonellaTyphi, this is also known as typhoid fever, after its incubation period (7–14 days), symptoms such as anorexia, headache, followed by general malaise and fever may occur. The interaction patient-causative agent is essential for the progression of the disease, salmonellamust find a microhabitat suitable for its establishment, multiplication and virulence factors expression.

Salmonellaproduces at least three toxins: enterotoxin, lipopolysaccharide endotoxin (LPS), and cytotoxin. Enterotoxigenicity, which is a property present in many serotypes of this microorganism, including S. Typhi, is expressed a few hours after contact with the host cell. The pathogenicity mechanisms by which salmonellainduces diarrhea and septicemia have not yet been clearly elucidated, but it appears to be a complex phenomenon involving numerous virulence factors such as those mentioned above.

The specific virulence factors are encoded by a group of genes for the formation of pathogenicity islands (SPI), with G + C percentages differing from the average of the bacterial genome. Direct repeats are present at the filament ends, carrying genes that encode mobility factors such as integrases, transposases or insertion sequences and are frequently inserted on tRNA. This suggests that they have been obtained from other species by horizontal transfer or by plasmids. There are numerous genes that participate in the invasion and that are present in salmonellas, genes that code for the synthesis of proteins related to the translocation of effector molecules within the cytoplasm of the host cell. Today, it is known that salmonellahas five islands of pathogenicity: SPI-1, SPI-2, SPI-3, SPI-4 and SPI-5 [10].

Advertisement

5. Mechanisms of resistance

Drug resistance and worldwide incidence of salmonellainfections has been increasingly reported. For example, it has been observed a high incidence among humans, livestock and poultry of Salmonella entericaserotype [4, [5],12:i:-], with variants ranging from sensitive- to multi-drug resistant, since the 1990s. Other examples include a strain of Salmonella entericadiscovered on 2015 that was provided with the gene mcr-1 of plasmid-mediated colistin resistance and clinical isolates from Portugal, China and United Kingdom observed in 2016 with this same gene [12].

Several types of salmonellawith multi-drug resistance (MDR) are capable of generating diverse types of plasmids, with gene cassettes that provide the property of resistance against antibiotics such as chloramphenicol, tetracycline, ampicillin, and streptomycin [13, 14]. The chromosomal mutation in the regions that determine the resistance to quinolones of the gyrA gene are responsible for the appearance of salmonellaserotypes with little susceptibility to ciprofloxacin [15]. On the other hand, the mutated genes that code for extended spectrum β-lactamases, are responsible for the serotypes that have begun to develop resistance to cephalosporins [16].

Resistance not only by salmonella, but by other microorganisms are currently a public health problem worldwide, which threatens the prevention, control and treatment of innumerable infectious diseases, having as expected consequences in terms of health and economic impact. This problem was recognized by the World Health Organization and in 2001 this organization published the Global Strategy for the Containment of Antimicrobial Resistance, publicizing interventionist actions to delay the appearance and to reduce the spread of resistant microorganisms [17]. For 2012, WHO proposed a series of actions such as strengthening health services and epidemiological surveillance, regulated use of antimicrobials in hospitals and in communities, promoting the development of new drugs and appropriate vaccines, among others [18]. This problem is one of the reasons for the development of new alternatives, being natural products derived from traditional medicine, one of the most used resources.

Advertisement

6. Traditional medicine and natural products

The origin of Natural and Traditional Medicine is indisputably linked both to human history and to its fight for survival [19]. Written evidence on plants being used as remedies for disease is as ancient as Mesopotamian tablets, and from there, a nearly endless number of registers in all cultures, supports its essential role on human well-being. Currently, traditional medicine has been delineated as the use of products of natural origin for health preservation, having the so-called Natural Products (NPs) at its focus.

NPs are broadly defined as small molecules produced by a living organism. This definition comprises a wide variety of compounds including the synthesized during basic metabolism (primary metabolites) or as by-products of it (secondary metabolites). Lipids, carbohydrates, proteins and nucleic acids are part of the first kind of NPs, while smaller molecules such as alkaloids, tannins, saponins and flavonoids are examples of secondary metabolites. Many of the latter does not seem to have a metabolic or evolutionary function for the parental organism, but regardless to that, its utility as drugs, preservatives, dyes, food additives and/or antibiotics is undeniable. Its application to counteract the pathogenic microorganisms affecting our specie, alongside side-effects and resistance to antibacterial drugs, is undoubtedly enough motivation for the current formalization and systematization of traditional knowledge, with methodological studies being carried out very frequently nowadays.

There has been an important upturn in the study of compounds of natural origin during the last decade, supported on ethnopharmacological information, folkloric reputation, traditional uses and the existence of previous evidence, and also based on NPs chemical composition and its chemotaxonomic classification. This explosion of information has been enriched primarily through the obtention and separation of crude extracts, essential oils, and/or other types of preparations that are subsequently analyzed for possible biological activities of metabolites or secondary products. Modern experimental strategies have included bioassays (mainly in vitro), development of NP libraries, production of active compounds in cell or tissue cultures, genetic manipulation of organisms, natural combinatorial chemistry, etc. [20]. NPs, being originated in living organisms, are essentially complex mixtures contained within cellular structures, hence the first step into the study of its properties is the separation of such structures. This first step is called extraction, and is generally carried out by liquid solvents at room temperature and atmospheric pressure, along with other well-known and widely used techniques such as steam distillation and the use of supercritical fluids or pressurized gases [21]. The proper choice of an extraction step is necessarily based on the nature, origin and composition of the product to be studied, taking into account the characteristics of the possible solvents (innokenty, reactivity, etc.), toxicity of secondary products, product sufficiency needs and evaluation methods to be followed afterwards, as a whole this step should result suitable to fulfill the objective of a research. Second and third steps are the setting of an adequate model for biological efficacy assessment and the elucidation of individual bioactive components.

In this chapter, we enlisted natural products frequently reported against salmonellafrom bacteria (Table 1), fungus (Table 2), animal (Table 3), plant (Table 4) or combined (Table 5) origin, organized on a chronologically descending order according to publishing date. To get a glimpse on the universe of information that NPs research has become, we made a fast search on two commonly used and easily accessible databases (PubMed and Google scholar) for the terms: salmonella, anti-salmonella, salmonellosis, natural productand antibacterial activity, alone or in combinations. Search results without the terms salmonellaor salmonellosiswere excluded. From the remaining registers, we selected those corresponding to experimental reports where the extraction step was performed and thoroughly described by authors. Studies on isolated or synthetic NPs were not included and research on infection or tissue damage protection after salmonellacolonization were also excluded. Review articles or abstracts were not considered, although we accounted congress and meeting proceedings where useful data were present. NPs and bioactive principles were registered according to the molecules isolated by the authors and/or in contrast to the literature. This search was not exhaustive, rather, we aim to obtain a random sample of information using the simplest terms on the matter of natural products for salmonellosis.

Parental organismOriginExtraction methodSegment usedBioactive compound(s)LocationSalmonellaserovarYearRef
Lactobacillus plantarum ZJ316Bacteriaculture supernatant filtration (methanol/acetonitrile)bacilliL-phenyl lactic acidChinaSalmonellaParatyphi-A (CMCC 50093), SalmonellaParatyphi-B (CMCC 50094), Salmonella enterica subsp. enterica (ATCC 14028), Salmonella enterica subsp. arizonae (CMCC(B) 47001), Salmonella choleraesuis (ATCC 13312), and SalmonellaTyphimurium (CMCC 50015)2020[22]
Lectin (Bifidobacterium adolescentis) from bee honeyBacteriacrude and purified extractshoneylectinIraqSalmonellaTyphi (clinical isolates)2019[23]
Lactobacillus salivarius, L. casei B1, L. plantarum, L. delbrueckii and L. delbrueckiiBacteriaco-cultureco-culturenot specifiedBeninSalmonella spp., SalmonellaTyphimurium (ATCC 14028)2019[24]
Lactococcus lactis subsp.lactis (CNRZ 1427)Bacterianot specifiednot specifiedspecific microbial enzymes, perox-ide, weak organic acids anti-bacterial peptides, secretion of bacteriocins protease productionAlgeriaSalmonella spp. (veterinary isolate). Mice tests2014[25]
Streptomyces sppBacteriacrude proteinmicrobial cellsnot specifiedIndiaSalmonellaEnteritidis2014[26]

Table 1.

Summary of frequently reported natural products from bacteria origin against salmonella.

Parental organismOriginExtraction methodSegment usedBioactive compound(s)LocationSalmonellaserovarYearRef
Lentinus edodesFungusfermenting/Black rice bran culturemyceliabioprocessed polysaccharideKoreaSalmonellaTyphimurium (SL1344)2018[27]
Coriolus versicolorFungusmethanolic extractnot specified (probably full fungi body)phenolics, polysaccharides, β-glucans, α-glucans, proteinsSerbiaSalmonellaEnteritidis (ATCC 13076)2016[28]
Pleorotus ostreatus (oyster mushroom)Fungusethanolic extractnot specified (probably full fungi body)not specifiedGermanySalmonellaTyphi2015[29]
Ganoderma lucidumFungusethanolic, methanolic, acetone and aqueous extractsfruiting bodiesnot specifiedIndiaSalmonellaTyphi (MTCC-531)2010[30]
Lentinus tuberregiumFungusHexane, Dichloromethane, Chloroform and Ethylacetate extractsnot specified (probably full fungi body)not specifiedIndiaSalmonellaFlerineri (M-1457) SalmonellaTyphi (M-733)2010[31]
Pichia pastoris X-33YeastYPD broth supplemented with 1 mg.mL − 1 pancreatin, 0.2% bile salts, and pH adjusted 8 with 0.1 N NaOHyeast cellnot specifiedBrazilSalmonellaTyphimurium (strain 29630)2015[32]

Table 2.

Summary of frequently reported natural products from fungi origin against salmonella.

Parental organismOriginExtraction methodSegment usedBioactive compound(s)LocationSalmonellaserovarYearRef
ApitoxinAnimalcrude apitoxinapitoxinMelittin, adolapin, apamin or MCD-peptide, phospholipase A2 or hyaluronidase, histamine, epinephrineEcuadorSalmonellaAnatum, Salmonella enterica subsp. arizonae, SalmonellaBardo, SalmonellaBredeney, SalmonellaDabou, SalmonellaDrac, SalmonellaEnteritidis, SalmonellaInfantis, SalmonellaIsangi, SalmonellaMontevideo, SalmonellaMbandaka, SalmonellaNdolo, SalmonellaNewport, SalmonellaRissen, S. enterica subespecie salamae, SalmonellaSeftenberg, S.Stanleyville, S.Thompson and SalmonellaTyphimurium2020[33]
ApitoxinAnimalcrude apitoxinapitoxinMelittin, adolapin, apamin or MCD-peptide, phospholipase A2 or hyaluronidase, histamine, epinephrineEcuadorSalmonellaNewport, Salmonella Isangi, Salmonella enterica subsp. salame, SalmonellaBardo, SalmonellaInfantis, SalmonellaMontevideo, SalmonellaStanleyville, SalmonellaNdolo, SalmonellaDabou, SalmonellaTyphimurium, SalmonellaEnteritidis2019[34]
Masske butterAnimallactic isolatesmicrobial cellslactic acidIranSalmonella enterica2019[35]
PropolisAnimalethanolic extractpropolisflavonoids, alkaloids, terpenoids, steroids, saponins, and tanninsIndonesiaSalmonella spp.2019[36]
Sarconesiopsis magellanicaAnimalRP-HPLClarvaeSarconesinColombiaSalmonella enterica (ATCC 13314)2018[37]
Dadih dadihAnimalice creambuffalo milk yogurtnot specifiedIndonesiaSalmonellaTyphimurium2017[38]
Donkey’s milkAnimalno extractionmilknot specifiedSerbiaSalmonellaEnteritidis (ATCC 13076)and SalmonellaTyphimurium (ATCC 14028)2017[39]
Colla corii asiniAnimalaqueous and ethanolic extractsdonkey-hide gelatinglycine, alanine, aspartic acid, glutamic acid, β-amino isobutyric acidKoreaSalmonellaTyphimurium (KCTC 1926)2017[40]
Bovine natural antibodiesAnimalantibodiesserumantibodiesThe NetherlandsSalmonellaTyphimurium (SL3261)2016[41]
PropolisAnimalethanolic extractspropolisphenolic acid components. Sinergy with cefiximeIndiaSalmonellaTyphimurium (MTCC 98)2016[42]
Anguilla spp.Animalaqueous dilutionmucusnot specifiedIndonesiaSalmonellaTyphi2016[43]
PropolisAnimalnot specifiedpropolisphenolic compounds (flavonoids)ChileSalmonellaEnteritidis2015[44]
HoneysAnimalaqueous dilutionhoneynot specifiedPakistanSalmonellaTyphi2015[45]
Platelet rich plasmaAnimalwhole bloodthrombin PRP/CaCl2 PRPprobably antimicrobial peptidesIranSalmonella enterica2014[46]
HoneyAnimalaqueous dilutionhoneynot specifiedRomaniaSalmonellaEnteritidis (ATCC 13076)2014[47]
Donkey’s milkAnimalno extractionmilknot specifiedSerbiaSalmonellaEnteritidis (ATCC 13076), SalmonellaTyphimurium (ATCC 14028), SalmonellaLivingstone2014[48]
PropolisAnimalethanolic, methanolic and aqueous extractspropolisterpenoids, flavonoids, alkaloids, phenols, tannins and saponinsIndiaSalmonellaTyphimurium2013[49]
Slovenian PropolisAnimal70% and 96% ethanolpropolisphenolic compounds (probably a synergy)SloveniaSalmonellaTyphimurium (14028), SalmonellaEnteritidis (ZM138)2012[50]
Shrimp ChitosanAnimalacetic acid 1%shrimpnot specifiedBangladeshSalmonellaParathypi2011[51]
Honeyanimalsaline dilutionhoneynot specifiedGreeceSalmonellaTyphimurium Salmonella enterica subsp.enterica (ATCC 13311)and SalmonellaTyphimurium and Salmonella2011[52]
PropolisAnimalethanolic extractpropolisquercetin, chrysin, 4′,5-dihydroxy-7-methoxyflavonone and 3,4′,7-trimethoxyflavononeTurkeySalmonellaEnteritidis (ATCC 13076)2011[53]
HoneyAnimalaqueous dilutionhoneynot specifiedIndiaSalmonella enterica serovarTyphi2010[54]

Table 3.

Summary of frequently reported natural products from animal origin against salmonella.

Parental organismOriginExtraction methodSegment usedBioactive compound(s)LocationSalmonellaserovarYearRef
Zanthoxylum Acanthopodium DCPlantn-hexane and ethyl acetate extractFruitflavonoids, alkaloids, and saponinsIndonesiaSalmonellaTyphi2020[55]
Aleurites moluccanaPlantmethanol extractsstem barkscopoletinIndonesiaSalmonellaTyphimurium2020[56]
Combre tummicranthum; Acacianilotica and Phyllanthus pentandrusPlantaqueous, ethanol and chloroform extractsleavestannins, flavonoids, saponins, sterols, triterpenes, alkaloids, anthocyanes and free anthraquinonesNigerSalmonellaTyphimurim, SalmonellaTyphi, Salmonella ParaTyphi, SalmonellaTyphimurim,and SalmonellaDerby2020[57]
Nauclea latifoliaPlantethyl acetate and methanolleavestannins, flavonoids and anthraquinones (all are highly polar and polyphenolic) as secondary metabolites but steroids were absentIndonesiaSalmonellaTyphi (clinical isolates, MDR)2020[58]
Hippobroma longifloraPlantethanolic extractsleavesalkaloids, flavonoids and saponinsSalmonellaTyphi2020[59]
Biarum bovei (cardin)Plantethanol 50% (ultrasound)leavesNerrel, flavonoids and nercernerrelIranSalmonellaEnteritidis (CMCC 50041)2020[60]
Trema orientalisL. Blumae (anggrung)Plantmethanol extractsleavesalkaloid, flavonoids, tannins, terpenoids, steroids, saponin, phenolicIndonesiaSalmonella spp.2020[61]
Agave tequilanaWeber var. azulPlantflourleavesFructansMexicoSalmonellaTyphimurium2020[62]
Clerodendrum fragransVent WilldPlantmethanol, ethyl acetate and n-hexane (chromatography)leavesTannins and flavonoidsIndonesiaSalmonella enterica (ATCC 14028)2020[63]
Canarium schweinfurthiiPlanthydro-ethanolic extract followed by chloroform and ethyl acetatestem barkmaniladiol, scopoletin, ethyl gallate and Gallic acidCameroonSalmonellaTyphi, SalmonellaEnteritidis and SalmonellaTyphimurium (clinical isolates)and SalmonellaTyphi (ATCC6539)2020[64]
Garcinia kolaand Alchornea cordifoliaPlanthydro-ethanolic and methanolic extractsleaves, root and stem barkAnthocyanins, Flavonoids, Glycosides, Phenols, Tannins, Triterpenoids and SteroidsCameroonS.Typhi (collection), S.Typhimurium and S.Enteritidis (clinical isolates)2020[65]
Ziziphus lotusand Ziziphus mauritianasPlantmethanolic extractsleaves, fruits and seedsQuinic acid, p-coumaric acid, rutin and quercitrinTunisiaSalmonellaTyphimurium (NRLB4420)2020[66]
Rhododendron arboreum and Justicia adhatodaPlantethanolic and methanolic extractsleavesoleanadien-3β-ethan-3-oateNepalSalmonella enterica subsp. enterica (ATCC 13076)2020[67]
Uvaria chamae, Lantana camaraand Phyllantus amarusPlantaqueous and ethanolic extractsleaves and rootnot specifiedBeninSalmonellaTyphimurium ATCC 14028and Salmonella spp. (isolates)2020[68]
Vitis viniferavar. AlbariñoPlanthydro-organic extraction (patented)fruitHOL: catechin, epicate-chin and isoquercetin. HOP: phologlucinic acid, miquelianin, rutin, inkaempferol and caftaric acidSpainSalmonella enterica subsp.enterica (CECT 554)2020[69]
Citrus hystrixPlantethanolic extractpeelnot specifiedIndonesiaSalmonellaTyphimurium2020[70]
Olive oilPlantethanolic extractfruitpolyphenol extractsChinaSalmonellaTyphimurium (ATCC 14028)2020[71]
Agrimonia pilosa Ledeb, Iris domestica(L.) Goldblatt and Mabb, Anemone chinensis Bunge,Plantaqueous extractsherb, rhizome, root and tubernot specifiedChinaSalmonellaEnteritidis (NCTC 0074, 1F6144, LE103and QA04/19)2020[72]
Litsea cubebaPlantessential oilfruit2,6-octadienal, 3,7-dimethyl-, 2,6-octadien-1-ol, 3,7-dimethyl-, and Z-2,6-octadien-1-ol, 3,7-dimethyl, Z-2,6-Octadienal, 3,7-dimethyl-, Z-citralChinaSalmonella enterica (CGMCC 1.755)2020[73]
Quercus infectoria, Phyllanthus emblicaPlantaqueous, methanolic and ethanolic extractsgall, fruithexadecanoid acid, 9-octadecenoic acid, octadecenoic acid, 2-tert buil-4-isopropil-5 metylphenolIndiaSalmonellaEnteritidis and SalmonellaTyphi2020[74]
Capparis deciduaPlantmethanolic extractwhole plantnot specifiedPakistanSalmonellaTyphi2020[75]
Detarium microcarpum Guill. & Perr.Plantethanolic extractleaves, twigs, roots, and root barkflavonoids, sterols, triterpenes, glucosides, coumarins, and saponinsCameroonSalmonellaTyphi (ATCC 19430), SalmonellaEnteritidis (ATCC 13076)2020[76]
Tetrapleura tetrapteraPlantethanolic extractstemcitral, acetic acid, limonene, butanol, 2-hydroxyl-3 butanone, Cis-Verbenol Trans-Verbenol, α-Terpinyl acetate, butanoic acid, 2-methyl butanolGhanaSalmonellaEnteritidis (CICC 21482)and SalmonellaTyphimurium (CICC 21483)2020[77]
Ocimum gratissimumPlantaqueous and ethanolic extractsleavesalkaloid, tannins, oxalate, flavonoids and essential oilNigeriaSamonellaTyphi and Salmonella ParaTyphi (clinical isolates)2019[78]
Aeollanthus pubescensPlantessential oil (aqueous)leavesthymol and carvacrol (anti-radical activity)NigeriaSalmonella spp. (multidrug resistant isolate)2019[79]
Annona muricataL.Plantethanol extractsflowersecondary metabolites such as alkaloids, phenolic and flavonoidIndonesiaSalmonellaEnteritidis2019[80]
Rhodomyrtus tomentosa(Ait) HasskPlantN-hexane, ethyl acetate, ethanolleavesphenols and flavonoidsIndonesiaSalmonellaTyphi2019[81]
Morinda lucidaPlantacetone and aqueous extractsleavesnot specifiedSouth africaSalmonella enterica subsp. enterica including S. enterica serovarGallinarum, Dublin, choleraesuis, Braenderup, Idikan, Kottbus,Typhimurium and Enteritidis2019[82]
Zanthoxylum acanthopodium DC (andaliman)Plantethanol extractsfruitsaponin, tannin, steroid and alkaloidIndonesiaSalmonellaTyphi2019[83]
Physalis peruvianaLPlantethanol extractsberries and leavesPhenolic compounds (1-hexanol, eucalyptol and 4-terpenol)EcuadorSalmonella spp. (clinical isolates)2019[84]
Carica papayaL.Plant70% ethanol, followed by n-hexane, ethyl acetate and waterseedsalkaloids, flavonoids, terpenoids and saponinsIndonesiaSalmonellaTyphi (ATCC 1408)2019[85]
Psidium guajavaPlantmethanol and aqueous extractsleaves and stem barkalkaloid, saponin, phenol, flavonoids, glycoside, anthraquinones, terpenoid and tanninNigeriaSalmonellaTyphi (clinical isolates)2019[86]
Artocarpus heterophyllus. Lamk.Plantethanol extractsleavesSaponin, flavonoids, terpenoid/steroids and tanninIndonesiaSalmonellaTyphi2019[87]
Sesbiana grandiflora L. PressPlant90% ethanol followed by n-hexane, ethyl acetate and aqueous extractionleavesSaponin, flavonoids, terpenoid, alkaloids and tanninIndonesiaSalmonellaTyphi2020[88]
Myristica fragransPlantaqueous extractseedsmethane, oxybis [dichloro-, 1H-Cyclopenta [c] furan-3-(3aH)-one,6,6a-dihydro-1-(1,3-dioxolan-2-yl)-,(3aR, 1-t, Octadecane, 6-methyl-, Heptadecane, 2,6,10,14-tetramethyl-, BIS (2-Ethylhextl) phthalate, 4H-Pyran-4-one,2,3-dihydro-3,5-dihydroxy- 6-methyl-, 3,4-Dichlorophenethylamine and 1,4-Benzenediol, 2-bromo-IndiaMDR SalmonellaTyphi isolates (MCASMZU1–13)2020[89]
Kalanchoe brasiliensisCambess.Planthydroethanolic extractleavesflavones and flavonols (3-hydroxyflavones or flavonols with substituted 3-hydroxyl groups (methylated or glycosylated))BrazilSalmonellaGastroenteritis2019[90]
White mustardPlantessential oilessential oilnot specified. Synergic with carvacrol and thymolUSASalmonellaTyphimurium2019[91]
Quercus variabilisBlumePlant70% ethanol followed by petroleum ether, ethyl acetate, n-butanol and watervalonia and shellellagic acid, theophylline, caffeic acid and tannin acidChinaSalmonellaParatyphi A, SalmonellaTyphimurium and SalmonellaEnteritidis2019[92]
Melia azedarachPlantethanol, ethylacetate, hexane, dichloromethane and methanol extractsleavesnot specifiedSyriaSalmonellaTyphi2019[93]
Ocotea minarumPlant80% ethanol followed by hexane and ethyl acetateleaves and stem barkcaffeic acid, p-coumaric acid, rosmarinic acid, quercetin and luteolinBrazilSalmonellaTyphimurium (14028), SalmonellaEnteritidis (13076)2019[94]
Zingiber zerumbetPlantethanolic extractrhizomeAlkaloids, terpenoids, and tanninsIndonesiaS.Enteritidis (ATCC 31194)and SalmonellaTyphimurium (ATCC 23564)2019[95]
Annona muricataPlantethanolic extractleavesflavonoids, alkaloids, terpenoids, saponins, coumarins, lactones,IndonesiaSalmonellaTyphimurium (FNCC-0050)2019[96]
Ligustrum lucidumAit, Lysimachia christinae Hance, Mentha piperita Linn and Cinnamomum cassiaPreslPlantaqueous extractsfruits, whole plants, leaves, and barksphenolic acid and flavonoidChinaS.Typhimurium (ST21) (used for prevent contracting infection)2019[97]
Pectin of Spondias dulcisPlantaqua, ethanolFruit peeloligosaccharidesCameroonSalmonellaTyphimurium (ATTC 2680), SalmonellaTyphimurium (ATTC 2488)and Salmonella choleraesuis2019[98]
Acacia farnesianaPlanthexanic, chloroform, methanolic and aqueous extractsfruitsmethylgallate, gallicacid and (2S)-naringenin-7-O-β-glucopyranosideMexicoSalmonellaEnteritidis (ATCC857)2019[99]
Adansonia digitataPlantaqueous, ethanolic and chloroform extractleaves and stem barkalkaloid, flavonoids and tanninNigeriaSalmonellaTyphi (clinical isolate)2018[100]
Cassia occidentalisPlantaqueous extractleavessaponin, flavonoids, and tannins, glycoside, cardiac glycosides, steroids, saponin glycoside, anthraquinones and volatile oil (trace)NigeriaSalmonellaTyphimurium2018[101]
Benincasa hispidaThunb (Bligo fruit)Plantethanol extracts (96, 70 and 50%)fruitnot specified (probably a polar molecule)IndonesiaSalmonellaTyphi2018[102]
Citrus sinensis(L) OsbeckPlantaqueous and ethanol (80%) extractspeelalkaloid, tannin, saponin, glycoside, flavonoid, terpenoid, and PhenolsNigeriaSalmonellaTyphi (clinical isolate)2018[103]
Cinammomum cassiaPlantSodium bisulfite (1:1), petroleum etheroilcinnamaldehydeIndonesiaSalmonellaTyphi2018[104]
Piper aduncum subsp. ossanum(C. DC.) Saralegui, Piper aduncum L. subsp. aduncum, Mentha piperita L., Mentha spicata L., Ocimum basilicum var. genovese L. Ocimum gratissimum L., Rosmarinus officinalis L., Thymus vulgaris L., Melaleuca quinquenervia (Cav) S.T. Blake, Eugenia axillarisL., Citrus sinensis (L.) Osbeck, Citrus paradisi Macfad, Curcuma longaL., Lippia graveolens (Kunth)Plantessential oil (aqueous)not specifiedProbably trans-cinamaldehyde, carvacrol, eugenol and acid 2,4 dihydroxybenzoicCubaSalmonellaTyphimurium (ATCC14028), Salmonella enterica subsp. enterica CENLAC (S02, S04, S06, S08, S10), Salmonella enterica (Sc1) isolated from a pig2018[105]
Jacaranda micranthaPlantaqueos and 70% ethanolextractleavesphenolic compounds, tannins, flavones and saponinsBrazilSalmonella choleraesuis (ATCC 10708)and Salmonella spp. (food isolated)2018[106]
Allium sativumand Zataria multiflora BoissPlantaqueous oil extractbulb and whole plantAllicin and thymolEgyptSalmonellaTyphimurium, SalmonellaAnatum, SalmonellaLagos and SalmonellaKentucky2018[107]
Cinnamomum zeylanicum, Eugenia caryophyllata, Origanum vulgare, Thymus vulgaris and Thymus zygisPlantessential oilbark, bud, flowering plant, leaves and flowerscinnamaldehyde, linalool, eugenol, eugenyl acetate, b-Caryophyllene, carvacrol, thymol, y-Terpinene, geraniol and p-Cymene.SpainSalmonellaTyphimurium (ATCC14028), SalmonellaTyphimurium and SalmonellaEnteritidis2018[108]
Citrus medica, Citrus limonand Citrus microcarpaPlantjuice (pure extract)fruitcitric acid, hesperidin, carvacrol and thymolKoreaSalmonellaTyphimurium (ATCC 14028, 19585,and DT104 Killercow)2018[109]
Equisetum telmateiaPlantethanolic extract followed by petroleum ether, dichloromethane (DCM), ethyl acetate (EtAc) and n-Butanol (n-BuOH). Supercritical extractstemKaempferol 3-O-(6”-O-acetylglucoside), 5-O-Caffeoyl shikimic acid, CatechinIranSalmonellaTyphi (PTCC 1609)2018[110]
Thymus vulgaris L., Rosmarinus officinalis L.Plantessential oilsleavesa-pinene, Thymol, Oxygenated monoterpenes, monoterpene hydrocarbons, borneol, 1,8-cineoleMoroccoSalmonellaTyphimurium (ATCC 14028)2018[111]
Gracilaria verrucosaPlant (algae)aqueous, methanolic and ethanolic extractswhole plantcarvacrol, p-cymene and y-terpineneIndonesiaSalmonellaTyphimurium2018[112]
Sterculia spp.Plantethanolic extractbarkflavonoids, alkaloids and saponinsIndonesiaSalmonellaTyphi2018[113]
Nigella sativaPlantaqueous and methanolic extracts and oilseednot specifiedPakistanSalmonella enterica2018[114]
Rice hull smoke extractPlantpyrolysis of rice hulls followed by liquefactionhull161 components, bioactive unknownKoreaSalmonellaTyphimurium (CCARM8107)2018[115]
Basil, ginger, hyssop, caraway, juniper, and sagePlantessential oilsseveralestragole, cis-pinocamphone, alpha-pinene (in juniper EO), a-thujone (in sage EO), carvone (in caraway EO) and curcumene (in ginger EO)SerbiaSalmonella enterica2017[116]
Ipomoea aquaticaPlantethanolic and methanolic extractsleavesflavonoidsMalaysiaSalmonellaTyphi2017[117]
Andrographis paniculataPlantmethanolic, ethanolic and acetone extractsleavesnot specifiedIndiaSalmonellaTyphi (clinical isolates)2017[118]
Senna occidentalisPlantmethanolic extractroot and leavesflavonoid, tannins, saponins, cardial glycosideNigeriaSalmonellaTyphi2017[119]
Grewia flavaPlantacetone, methanolic, acetylacetate and aqueous extractsberries, leaves, bark and rootspelargonidin 3,5-diglucoside, naringenin-7-O-β-D-glucoside, tannins, catechins, and cyanidin-3-glucoside, betulin, lupeol, lupenone and friedelin.SouthSalmonellaTyphimurium (ATCC 14028)2017[120]
Acacia mearnsiiDe Wild., Aloe arborescensMill., A. striataHaw., Cyathula uncinulata (Schrad.) Schinz, Eucomis autumnalis (Mill.) Chitt., E. comosa(Houtt.) Wehrh., Hermbstaedtia odorata(Burch. ex Moq.) T.Cooke, Hydnora africana Thunb, Hypoxis latifolia Wight, Pelargonium sidoides DC, Psidium guajava L and Schizocarphus nervosus (Burch.) van der MerwePlantacetone extractbark, leaves, bulb, tuber, root and cormsquercetin-3-O-a-l-arabinopyranoside (P. guajava)South AfricaSalmonella Isangi, SalmonellaTyphi, SalmonellaTyphimurium2017[121]
Holarrhena floribundaPlantethanolic and methanolic extractsleavesAlkaloidsTogoSalmonellaTyphi (clinical strains)2017[122]
Zanthoxylum caribaeumLam.Plantethanolic, methanolic, hexanic, acetone, dichloromethanic, ethylacetate and aqueous extractsleavesGermacrene-D, a-Panasinsene and b-SelineneBrazilSalmonella enterica2017[123]
Rosmarinus officinalisPlantessential oilnot specifiednot specifiedIranSalmonellaTyphimurium (PTCC 1609)2017[124]
Myristica fragansaqueous extractsseedmethane, oxybisIndiaSalmonellaTyphi2017[125]
Cajanus cajan(Gandul)Plantmethanolic extractleavesflavonoids, phenolics, and steroids (naringenin)IndonesiaSalmonella Thypi2017[126]
Vitex donianaPlantaqueous and methanolic extractsstem-bark and leavesphytochemicals alkaloid, saponin, tannin, anthraquinone, flavonoid, phenols, terpenoid andglycosideNigeriaSalmonellaTyphi2017[127]
Hibiscus sabdariffaPlantaqueous waterflower calyxnot specifiedMexicoSalmonellaTyphimurium and Typhi2017[128]
Ziziphora clinopodioidesPlantessential oilleavesnisinIranSalmonellaTyphimurium (ATCC 14028)2017[129]
Tinospora cordifoliaPlantaqueous and methanolic extractsstemnot specifiedIndiaS.Typhimurium (ATCC 23564)2017[130]
Sonchus arvensisL. (tempuyung)Plantethanol extractsleavesflavonoids and triterpenoidsIndonesiaSalmonellaTyphi2016[131]
Heliotropium filifolium(Miers) Reiche and of Heliotropium sinuatum(Miers)Plantresinous exudate (CH2Cl2) and hexane-ethyl acetate step gradientfresh parts (cuticular components)(Filifolinol) (naringenin, 3-O-methylgalanginand pinocembrin)ChileSalmonellaTyphimurium (ATCC 14028)2016[132]
Punica granatum, oak, Thymus vulgaris and Cinnamomum zeylanicumPlantethanolic and chloroformic extractspeel, oak trunk, thyme fruit and cortexgallocatechins, delphinidin, cyanidin, gallicacid,ellagic acid, pelargonidin and sitosterol; hymol, carvacrol and flavonoids; cynamaldheide; (4,5-Di-o-galloyl (+) –protoquercitol) and compound III (3,5-Di-o-galloyl (+)-protoquercitoIraqSalmonellaTyphimurium (chicken isolate)2016[133]
Scutellariae radixPlantethanol extracts followed by petroleum ether (PEF), chloroform (CF), ethyl acetate (EAF) and n-butanol (BF)rootbaicalin, wogonoside, baicalein and wogoninChinaSalmonellaTyphimurium (CMCC 50041)2016[134]
Rhus typhinaand Achillea sintenisiiPlantnot specifiedaerial and root partsnot specifiedPortugalSalmonellaTyphimurium LT22016[135]
Holarrhena antidysentrica (Ha) and Andrographis paniculata (Ap)Planthydroethanolic extractleaves and stemalkaloids, flavonoids, saponin, terpenes, phenols, tannins, glycosides carotenoids, anthraquinones, reducing sugars, phlobatannins, sterolsIndiaSalmonellaTyphimurium (MTCC 733)2016[136]
Black tea (Kombucha)PlantInfusion/fermentationleavesCatechin and isorhamnetinIndiaSalmonellaTyphimurium (NCT 572)2016[137]
Curcuma longaPlant96% ethanol/essential oilrhizomessaponin, tannins, alkaloids and flavonoids (probably curcumin and derivatives)ColombiaSalmonella spp. (nosocomial isolates)2016[138]
Harungana madagascariensisPlantaqueous infusionleavesnot specifiedCameroonSalmonellaTyphimurium2016[139]
Piper retrofractum, Phyllanthus emblica, Terminalia chebula, Terminalia bellirica, Piper sarmentosum, Plumbago indica, Piper leptostachyum, Piper nigrum, Zingiber officinale, Piper betle, Garcinia mangostanaand Caesal piniasappanPlant95% ethanolfruits, root, stem, rhizome, leaves husk, peduncle and woodPlumbagin, Piperine, Eugenol, Myristicin, Gingerol, Shogaol and BrazilinThailandSalmonella spp. (piglet isolates)2016[140]
Punica granatumPlantethanolic extracts and peel flourpeel, seedsellagic acid or ellagic acid derivatives, ellagitannins and HHDP-gallagyl-hexosideSpainSalmonellaAnatum, SalmonellaTyphimurium2016[141]
Abrus precatoriusL.Plantaaqueous extractsleaves, seed and rootsteroids, saponins, phenolics, tannins, flavonoids, terpenoids and alkaloidsNigeriaSalmonellaTyphi2016[142]
Piliostigma thonningiiPlanthexane and aqueous extractsleavesTannins, terpenoids, flavonoids, alkaloids, steroids and phenolsNigeriaSalmonellaTyphi2015[143]
Baillonella toxispermaPlantethyl acetate, acetone, methanol and hydro-ethanol mixture (2: 8) extractsleaves and stem barkterpenoids, tannins, flavonoids, phenols, saponins, steroids and cardiac glycosides.CameroonSalmonellaTyphi2015[144]
Wood vinegarPlantvinegarnatural vinegarnot specified (probably pH 4.15–4.59)ThailandSalmonellaEnteritidis (DMST15676) SalmonellaTyphimurium (DMST17242)2015[145]
Aristolochia indica, Carica papaya, Eclipta albaand Phyllanthus amarusPlantmethanol extractsleavesn-Hexadecanoic acidIndiaSalmonellaTyphi (clinical isolate)2015[146]
Curry: Capsicum annuum, Citrus hystrix, Cuminum cyminumL., Allium ascalonicum L., Allium sativum, Cybopogon citratus, Alpinia galangal, ando coconut milkPlantwater, UHT coconut milk, and fresh coconut milk were used as extractants. Also ethanolic and aquous extracts (Garlic)fruit, leaves and peelnot specifiedThailandSalmonellaEnteritidis2015[147]
Portulaca oleraceaPlantethanol extractsleavesprobably quercetinThailandSalmonellaTyphi2015[148]
Eucalyptus, mint, cinnamon, garlic, thymusPlantoilbark and leavesprobably cinnamaldehide/thymolEgyptSalmonellaEnteritidis, SalmonellaCharity and SalmonellaRemiremont (chicken isolates)2015[149]
Piper crocatum (Red betel vine)Plant70% ethanol, followed by n-hexane, ethyl acetate, chloroform and methanolleavessaponin and flavonoidsIndonesiaSalmonellaTyphi2015[150]
Dionisya revolutaPlantmethanol extractsaerial partsnot specifiedIranSalmonellaEnteritidis2015[151]
Achyranthes asperaPlantmethanolic extracts followed by chloroform, n-hexane, n-butanol, ethyl acetate and waterleavesPhenolic compounds, oils, saponins, flavonoids, alkaloids and tanninsPakistanSalmonellaTyphi (ATCC 19430)2015[152]
Alocasia brisbanensis, Canavalia rosea, Corymbia intermedia, Hibbertia scandens, Ipomoea brasiliensis, Lophostemon suaveolens, Syncarpia glomulifera, Smilax australis and Smilax glyciphyllaPlanthydro-ethanolic (80%) and aqueous extractsnot specifiedL. suaveolensleaves: α-pinene, β-caryophyllene, aromadendrene, globulol and spathulenol; S. glomulifera: α-pinene, aromadendrene and globulol; S. glomulifera leaves wax: eucalyptin and S. glomulifera bark: betulinic acid, oleanolic acid-3-acetate and ursolic acid-3-acetate.AustraliaSalmonella typhimurium—Group B (clinical isolate)2015[153]
Citrus sinensisPlantethanol, methanol, chloroform, and diethyl etherpeelsaponins, terpenoids, slkaloids, flavonoids, tannins and cardiac glycosidesPakistanSalmonellaTyphimurium (isolated from spoiled fish)2015[154]
Nigella sativaPlantessential oilseedthymoquinone, p-cymene, a-phellandrene, a-pinene, b-pinen, cis-carveol, trans-anethole, thymol, alongipinene and longifoleneArabia and IndiaSalmonellaParatyphi A, SalmonellaEnteritidis, SalmonellaTyphimurium, SalmonellaHeidelberg, SalmonellaAgona, Salmonella bongori2015[155]
Allium sativum L.Plantaqueous extractsbulbnot specifiedSouth KoreaSalmonellaTyphimurium2015[156]
Spirulina platensisPlant (algae)ethanolic and chloroform extractscell extractsnot specifiedBangladeshSalmonellaTyphi and SalmonellaParatyphi2015[157]
Curri = Capsicum annuum, Citrus hystrix, Cuminum cyminum L., Allium ascalonicum L., Allium sativum, Cybopogon citratus, Alpinia galangal, ando coconut milkPlantnot specifiedfruit, peel, seed, bulb, stem, rhizomeespecificados por compuesto, reportes previosThailandSalmonellaTyphimurium (DT104b)2015[158]
Vitex donianaPlantethanolic and Acetone extractsleave, stem bark and roottannin, saponins, flavonoid, carbohydrate, glycoside, protein and steroidNigeriaSalmonellaTyphi2015[159]
Polygonum odoratumPlantessential oilleavesDodecanal 55.49%, Decanal 11.57%, Pentacosane 7.26%, p-Anis aldehyde 6.35% mainlyThailandSalmonella choleraesuis subsp. choleraesuis (ATCC 35640)2015[160]
Kelussia odoratissimaPlantaqueous and ethanolic extractsleavesnot specifiedIranSalmonella typhimurium (ATCC 14028)2014[161]
Coptidis rhizoma (CR), Houttuyniae herba, Taraxaci herba, Glycyrrhizae radix, Puerariae radix, and Rhizoma dioscoreaePlantaqueous infusionherbsberberine, ginsenoside Rb1, and glycyrrhizinChinaSalmonellaTyphimurium (ATCC 6994)and ST21 (pig carrier)2014[162]
Nymphea tetragonaPlant50% methanol followed by dichloromethane, ethyl acetate, and butanolbody and rootDFNTE: hydrocarbons (46.46%); EFNTE: methyl gallate (70.44%), 1, 2, 3-benzenetriol or pyrogallol (20.61%), and 6, 8-dimethylbenzocyclooctene (5.90%); BFNTE: 2-hydrazinoquinoline (57.61%), pyro-gallol (20.09%), and methyl gallate (12.77%)KoreaSalmonellaTyphimurium (QC strain KTCC2515and clinical isolates ST171, ST482, ST688,and ST21)2014[163]
Virgin coconut oil and palm kernel oilPlantessential oilfruit and seednot specifiedIndonesiaSalmonellaTyphi (ATCC 786)2014[164]
Virgin Coconut OilPlantoilfruitnot specifiedIndonesiaSalmonellaTyphi (ATCC 00786)and SalmonellaTyphimurium (ATCC 14028)2014[165]
Piper nigrum L.Plantethanolic extracts and chloroform extractsfruit and seedtannins, alkaloids and Cardiac glycosides, and tannins, alkaloids and flavonoidsIndiaSalmonellaTyphi2014[166]
Morus albavar. Alba, Morus alba var. Rosa and Morus rubraPlanthydromethanolic and aqueous extractsleaves and stemphenolics and flavonoidsTunisiaSalmonellaTyphimurium (ATCC 14028)2014[167]
Khaya senegalensisPlantethanolic, methanolic and aquous extractsstem barksaponins, tannins, reducing sugar, aldehyde, phlobatannins, flavonoids, terpenoids, alkaloids, cardiac glycoside and anthroquinonesNigeriaSalmonellaTyphi2014[168]
Mentha longifoliaPlantEthanolic extractsleavesnot specifiedIranSalmonellaTyphimurium2014[169]
Palm oil (Sania), Virgen coconut oil (Palem Mustika) and soybean oil (Mama Suka)Plantoilsseeds and fruitsnot specifiedIndonesiaSalmonellaTyphi (ATCC 19943)2014[170]
HeliotropiumPlantmethanolic extract, 2nd extraction with petroleum ether, ethylacetate and chloroform and aqueousaerial partsnot specifiedIranSalmonellaEnteritidis (ATCC 13311)2014[171]
Woad, heartleaf houttuynia herb, baical skullcap, coptidis, andrographitis,Plantaqueos extractbark, leaves, root, rhizome and fruitnot specifiedChinaSalmonellaTyphimurium2013[172]
K. senegalensisbark and leaves, S. alexandrinaleaves, S. argel leaves, T. indicaL. fruits and T. foenum, graecum seedsPlantmethanolic extractbark, leaves and seednot specifiedSudanSalmonellaTyphi(ATCC19430)and Salmonella Paratyphyphi-A (ATCC 9150 / SARb42)2013[173]
Phylanthus amarusPlantaqueous and ethanolic extractsleavesPhyllanthin, Nirtetralin, Linalool, phytolIndiaSalmonellaTyphi2013[174]
Carissa opacaPlant95% methanol followed by n-hexane, ethyl acetate, chloroform, butanol and waterfruitsorientin, isoquercetin, myricetin and apigenin (and probably other secondary metabolites)PakistanSalmonella typhy (ATCC 0650)2013[175]
Mangifera indicaPlantacetone extractleavesmangiferinPakistanSalmonellaTyphi (clinical isolates)and Salmonella (ATCC 14028)2013[176]
Sinapis albaL.Plantessential oilseeds4-hydroxybenzyl isothiocyanateUSASalmonella spp. (isolates) SalmonellaTyphimurium (ATCC 14028), SalmonellaAbaetuba and SalmonellaDessau2013[177]
Origanum vulgarePlantessential oilseednot specifiedUSASalmonellaNewport (LAJ160311)2013[178]
Annona comosus and Citrus senensisPlantethanolic extractpeelalkaloids, flavonoids, saponins, tanninsNigeriaSalmonella paratyphi-B, and SalmonellaTyphi2013[179]
Carthamus nctoricus L., Poncirus trifollata Raf., Scutellaria balcalensis Georgi, Prunus sargentii, Cucurbita moschata, Allium cepaL., Portulaca oleracea L., Xanthium strumarium L., Duchesnea chrysantha, Cudrania tricuspidataand Juniperus chinensisPlantethanolic extractleaves, peelnot specifiedKoreaSalmonellaGallinarum2013[180]
Herba pogostemonisPlantaqueous extractleavesacetol,D-sphignosin, 5-aminoimidazole-1-carboxyamie, caffeic acid,chlorogenic acid, neohesperedin,O-acetylsalicylic acid, quinic acid,3,4-dihydroxybenzoic acid, andDL-hydroxyphenylglycolKoreaSalmonellaTyphimurium (ATCC140)2012[181]
Enicostemma littoralePlantchloroform, methanol and acetone by soxhlet apparatusleaves, stem and rootnot specifiedIndiaSalmonellaTyphi2012[182]
Hibiscus rosa-sinensisPlantaqueous and ethanolic extractsflower extractcyanidin, quercetin, hentriacontane, calcium oxalate, thiamine, riboflavin, niacin and ascorbic acidsIndiaSalmonella spp.2012[183]
Capsicum annuum and Capsicum frutescensPlantaqueous and methanolic extractsfruitalkaloids, flavonoids, polyphenols, and sterolsIvory CoastSalmonellaTyphimurium (ATCC 13311)2012[184]
Coriandrum sativam (L.)Plantessential oilfruit dryBicycle(4.1.0),heptanes,3,7,7-trimethyl-(1a,6a,3a), propanoic acid,2-methyl-3,7-dimethyl octadiennyl ester,(E)-, 2- undecenal, 2-Napthalenemethanol, decahydro-a,a,4a-trimethyl-8-methylene- [2R-(2a,4aa,8aa)]IndiaSalmonellaTyphi2012[185]
Berberis baluchistanica, Seriphidium quettense, Iphionaaucheri, Ferula costataPlantcrude methanol extractsroots, aerial partsnot specifiedPakistanSalmonellaTyphimurium2012[186]
Oenothera roseaPlantaqueous and ethanolic extractsaerial partsnot specifiedMexicoSalmonellaEnteritidis (clinical isolate)2012[187]
Ocimum gratissimum and Gongronema latifoliumPlantaqueous and ethanolic extractsleaves and stemnot specifiedNigeriaSalmonellaTyphi2012[188]
Curry: Capsicum annuum, Citrus hystrix, Cuminum cyminum L., Allium ascalonicum L., Allium sativum, Cybopogon citratus, Alpinia galangal, ando coconut milkPlantKaeng Kathi (UHT coconut milkfruit, peel, seed, bulb, stem, rhizomenot specifiedIndonesiaSalmonellaTyphimurium U302 (DT104b)2012[189]
Averrhoa bilimbiLinnPlantethanolic extractleavesnot specifiedIndonesiaSalmonellaTyphi2012[190]
Ocimum gratissimumPlantethanol extractsleavesalkaloids, cardiac glycosides, saponins, tannins and steroidsNigeriaSalmonellaTyphi (clinical isolates)2011[191]
Ardisia ellipticaThumbPlant95% ethanolfruitanythocyanins and syringic acidThailandSalmonella sp.2011[192]
QuercusPlantethanol by soxhlet apparatusacornnot specifiedIranSalmonellaTyphi (MDR)2011[193]
Sonchus spp. (6 sp) S. arvensis, S. oleraceus, S. Lingianus, S. Brachyotus, S. asper, S. uliginosusPlantmethanolic extractaerial partsphenols and flavonoidsChinaSalmonella enterica2011[194]
York cabbage, Brussels sprouts, broccoli and white cabbagePlantmethanolic extractwhole plantHydroxybenzoic acid, hydroxycinnamic acid, flavone, polymethoxylated flavone, glycosylated flavonoid and anthocyaninIrelandSalmonella Abony (NCTC 6017)2011[195]
Achyrocline satureioidesPlantethanolic extractaerial parts23-methyl-6-OdesmethylauricepyroneArgentinaSalmonellaEnteritidis2011[196]
Trapa bispinosaRoxbPlantmethanolic extractfruitnot specifiedBangladeshSalmonellaTyphi2011[197]
Acalypha indicaPlantmethanolic extractleaves and rootsnot specifiedIndiaSalmonellaTyphi2011[198]
Punica granatumPlantethanolic extractpeelnot specifiedKoreaSalmonellaTyphi (ATCC 19943), S.Dublin (ATCC 39184), S.Derby (ATCC 6960), S. choleraesuis (ATCC 7001) y S.Gallinarum (ATCC 9184), S.Enteritidis, S.Typhimurium, S.Gallinarum y S. Paratyphi A2011[199]
Punica granatum L.. Eugenia jambolana Lam., Eugenia unifloraL., Caryophyllus aromaticus L., Psidium araca Raddi, Achyrocline satureioides (Lam.), Rosmarinus officinalis L., Cynara scolymus L., Salvia officinalis L., Laurus nobilis L., Bidens pilosa L., Baccharis trimera (Less.) DC, Plectranthus barbatusAndrews, Sonchus oleraceusL., Mikania glomerata Spreng., Taraxacum officinaleF.H. Wigg, Emiia sonchifolia (L) DC, Plantago australisLam., Maytenus ilicifolia (Schrad) Planch, Aloe arborescens Mill., Malva sylvestrisL.Planthydromethanolic extractsleaves, fruit, package content, aerial and flowered aerial portions.not specifiedBrazilSalmonellaAgona, SalmonellaAnatum, SalmonellaCerro, CerroCubana, SalmonellaDerby, SalmonellaEnteritidis, Salmonell Give, SalmonellaHeidelberg, SalmonellaInfantis, SalmonellaLondon, SalmonellaManhattan, SalmonellaMeleagridis, SalmonellaMontevideo, SalmonellaNewport, SalmonellaOranienburg, SalmonellaPanama, SalmonellaPullorum, SalmonellaTyphimurium2011[200]
Cucurbita pepoPlantmethanolic and ethanolic extractsseedsaponins, flavonoids, Tannins, alkaloids, and steroidsNigeriaSalmonellaTyphi2011[201]
Aloe veraPlantmethanolic and ethanolic extractsleavesAnthraquinone, Alkaloids, Saponins, Balsams, Flavonoids and TanninsNigeriaSalmonellaTyphi2011[202]
Gynostemma pentaphyllumPlantethanolic extractleave, stemnot specifiedThailandSalmonellaTyphi SalmonellaTyphimurium2011[203]
Terminalia stenostachya y TerminaliaspinosaPlantdichloromethanic, methanolic, acetone and ethanolic extractsstem barks and rootsnot specifiedTanzaniaSalmonellaTyphi2011[204]
Hofmeisteria schaffneriPlantinfusion and essential oilaerial partshofmeisterin III, thymyl isovalerate and 8,9-epoxy-10-acetoxythymyl angelateMexicoSalmonellaTyphi (ATCC9992)2011[205]
Ficus polita Vahl.Plantmethanolic extractrootseuphol-3- O -cinamato C 39 H 56 O 2, lupeol C 30 H 50 O, taraxar-14-eno C30 H 50 O 1CameroonSalmonellaTyphi (ATCC6539)2011[206]
Aegle marmelos(L.) Corr. Serr. (Rutaceae), Cassia fistulaL., Moringa oleifera Lam., Melia azedarach L., Bombax ceiba L. and Brassica rapa ssp. campestrisL.Plantaqueous and methanolic extractsvegetables, seedsnot specifiedPakistanSalmonellaTyphi2011[207]
75 plants (Healianthus annum Linn.)Plantethanolic extractsleavesnot specifiedIndiaSalmonellaTyphosa2010[208]
Syzygium cuminiPlantaqueous and ethanolic extractsleavesflavonoids, alkaloids,IndiaSalmonellaEnteritidis, SalmonellaTyphi, SalmonellaTyphi A,Salmonella paraTyphi A, Salmonella paraTyphiB2010[209]
Black pepper (Piper nigrum Linn.)Plantacetone extract; dichloromethanic extract;fruitpiperineIndiaSalmonellaTyphi2010[210]
Abrus precatorius L.Plantmethanolic and petroleum ether extractleaves, seeds and rootsMethanolic and petroleum ether extractIndiaSalmonellaTyphi, Salmonella Paratyphi A, Salmonella Paratyphi B2010[211]
Psidium guajavaPlantmethanolic extractleavesflavonoids: morin-3-Olyxoside, morin-3-O-arabinoside, quercetin-3-Oarabinoside and quercetin. Anthocyans, alkaloids, tannins, and terpenoids.ThailandSalmonella enterica (ATTC 8326)2010[212]
Origanum vulgare L.Plantessential oilsaerial partsnot specifiedTurkeySalmonellaEnteritidis RSKK 96046,2010[213]
Tectona grandisPlantmethanolic extractleavesnot specifiedIndiaSalmonellaTyphimurium (MTCC 982010[214]
Ocimum canum, Acalypha indica, Eclipta alba and Lawsonia inermisPlantchloroform and methanolwhole plantnot specifiedIndiaSalmonella paraTyphi2010[215]
Adiantum capillus-venerisL.
(Adiantaceae), Adiantum incisum forsk. (Adiantaceae), Adiantum lunulatum Burm. F. (Adiantaceae), Actiniopteris radiata (Swartz.), Enlace (Actiniopteridaceae), Araiostegia pseudocystopteris Copel. (Davalliaceae), Athyrium pectinatum (Wall ex Mett.) T. Moore (Athyriaceae), Chelienthes albomarginata Clarke (Sinopteridaceae), Cyclosorus dentatus(Forsk.) Ching (Thelypteridaceae), Dryopteris cochleata (Don.) C. Chr. (Dryopteridaceae), hipodematio crenatum (Forsk.) Kuhn (Hypodematiaceae), Marsilea minutaL. (Marsileaceae) y Tectaria coadunata (J. Smith)
Plantaqueous and methanolic extractsleavesnot specifiedIndiaSalmonella arizonae (MTCC No. 660), SalmonellaTyphi (MTCC No. 734)2010[216]
Ecklonia cavaPlant (algae)EtOH followed by n-hexane, CH2Cl2, EtOAc, n-BuOH (10 g) and aqueousnot specified (probably full algae)EckolKoreaSalmonellaTyphi (ATCC 19943), SalmonellaDublin (ATCC 39184), SalmonellaDerby (ATCC 6960), Salmonella choleraesuis (ATCC 7001), SalmonellaGallinarum (ATCC 9184), SalmonellaEnteritidis, SalmonellaTyphimurium, S.Gallinarum, and SalmonellaParatyphi A2010[217]
Thymus vulgaris L., Ocimum basilicum L., Coriandrum sativum L., Rosmarinus officinalis L., Salvia officinalis L., Foeniculum vulgare L., Mentha spicata L., Carum carvi L.Plantessential oilnot specifiednot specifiedRomaniaSalmonella enterica serovarEnteritidis Cantacuzino CICC10878, Salmonellaenterica serovarEnteritidis2010[218]
Pikutbenjakul = Piper longum, Piper sarmentosum, Piper interruptum, Plumbago indica y ZingiberPlantethanolic extractnot specifiednot specifiedThailandSalmonella sp. Salmnella typhy amd salmonellaTyphimurium2010[219]
Quercus infectoria, Kaempferia galanga, Coptis chinensis and Glycyrrhiza uralensisPlantDMSOgalls, roots, rhizomes,not specifiedThailandSalmonellaTyphi (DMST 5784)2010[220]
EugenolPlantessential oil of cloveflower extract177 peaks and HHDP-gallagyl-hexosideIndonesiaSalmonellaTyphi2010[221]
Sida rhombifoliaLinn.Plantmethanolic extractnot especifiedpolyphenols, alkaloids and steroidsCameroonSalmonellaTyphi, SalmonellaEnteritidis2010[222]

Table 4.

Summary of frequently reported natural products from plant origin against salmonella.

Parental organismOriginExtraction methodSegment usedBioactive compound(s)LocationSalmonellaserovarYearRef
Goat milk kefir (Lactococcus cremoris, Streptococcus cremorisBacteria and Yeastno extractionmicrobial cellslactic acid, ethanol and CO2, diacetyl acetaldehyde, ethyl andIndonesiaSalmonellaTyphimurium (ATCC 14028)2019[223]
Epicoccum nigrum, Entada abyssinicaFungus and plantethyl acetate extractleavesnot specifiedCameroonSalmonellaTyphimurium2017[224]
Origanum vulgare, Lactococcus lactis (Nisin), EDTAPlant and Bacteriaessential oilseedscarvacrol, p-cymene and y-terpineneBrazilSalmonellaEnteritidis2016[225]
Allium sativum, Nigella sativa, Azadirachta indica, Ficus carica, Trigonella foenum-graecumand honeyPlant and Animalaqueous extractsbulb, seed, leaves and fruitnot specifiedPakistanSalmonella spp.2014[226]
Apis mellipodae honey and Allium sativumAnimal and plantmacerated and aqueous dilutionhoney/bulbHoney: high, osmolarity, hydrogen peroxidase, acidity and Allium sativum: allicinEthiopiaSalmonellaTyphi (clinical isolate)and Salmonella spp. (NCTC 8385)2013[227]

Table 5.

Summary of reported natural products of combined origins against salmonella.

All these works were developed on all continents, being Asia the most active, followed by Africa, America, Europe and Oceania (Figure 1A and B). It is noteworthy that much of the research was developed in equatorial locations where biodiversity is abundant. Country-wise, there is a remarkable number of publications from India and Indonesia, where incidence of salmonellais high. The map constructed for the distribution of publishing frequencies, in fact, resulted fairly similar to a previously reported salmonellaincidence map (Figure 1A versus [9]). The number of articles per year showed an upward trend though it stabilizes in the last five years (Figure 1C).

Figure 1.

(A) Distribution map of publishing frequencies. (B) Continental frequency. (C) Publications per year.

The spectrum of biological activities evaluated are as diverse as the application to which they are oriented, from the study of antimutagenic, antioxidant, anticancer, anthelmintic, antiviral, antifungal activities to its antibacterial potential, being its activity against salmonellaspp. one of the most studied activities.

The analysis of the last decade research render studies exploring the antibacterial activity against salmonellaserovars of crude extracts and essential oils, from compounds of natural origin, as well as their components. A wide variety of these NPs have been evaluated from commercial formulations, products of animal origin such as honey, propolis, milk and chitosan, through complete plants and/or their components (roots, stem, leaves and flowers), up until products of microbial metabolism as crude protein extracts, membrane and cell wall glycosides, natural antibiotic peptides (nisin). Several chemical compounds such as water, ethanol, methanol, acetone, formaldehyde, hexane, ethyl acetate and chloroform were used as solvents by direct maceration extraction rather than vapor distillation or more complex methods.

Nonetheless, we believe the description of methodological conditions could further standardized with the inclusion of a fixed set of data. According to our observation, the list of items enlisted in Table 6 could be a minimal checklist when performing NP research.

Animal speciesVegetable speciesMicroorganismsSolventsBioactive compound
Identification of genus and, if possible, speciesGeographical siteIdentification of genus, and speciesExplanation for its selectionIsolation technique
Harvesting dataReference strain identificationNo-reactivity assessmentStructure determination method (MS–GC, for instance)
Ethnobiological identificationIdentification of origin:
  • Clinical isolate

  • Food

  • Soil…

No-interference assessment
Identification by PCRNo-toxicity assessment

Table 6.

Checklist proposed for NPs research.

Advertisement

7. Conclusions

Salmonellosis, caused by salmonellaserovars, is still an uneradicated disease both in industrialized and developing countries. Multidrug resistance is a phenomenon increasingly widespread and alternative tools for disease control are urgently necessary. Natural products research based on traditional medicine is nowadays a consolidated study field full of vitality, salmonellaresearch in particular has an upward trend with work being develop worldwide. Authors cited within this chapter explored biological activities of local organisms for the solution of salmonellosis for their communities, although a minority showed interested in foreign resources or commercial formulations. We observed a higher number of active researches on countries with diverse and abundant natural resources coincidentally also with high salmonellosis incidence. Even though our search is a minimal sample from the whole work being published on NPs and salmonellosis, it reveals certain features of the field.

Most of the works displayed in here are initial screening in vitro studies, maybe due to the scarce number of sources for funding in vivo applications. In perspective, NPs studies for clinical applications is a potential goal in order to control this disease.

Advertisement

Acknowledgments

Authors acknowledge Hospital Juarez of Mexico for giving the facilities for writing this chapter. This work was developed with no funding.

Advertisement

Conflict of interest

Authors declare no conflict of interests.

© 2021 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution 3.0 License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

How to cite and reference

Link to this chapter Copy to clipboard

Cite this chapter Copy to clipboard

Nancy Jannet Ruiz-Pérez, Jaime Sánchez-Navarrete and Julia D. Toscano-Garibay (March 1st 2021). Natural Products for Salmonellosis: Last Decade Research, Salmonella spp. - A Global Challenge, Alexandre Lamas, Patricia Regal and Carlos Manuel Franco, IntechOpen, DOI: 10.5772/intechopen.96207. Available from:

chapter statistics

151total chapter downloads

More statistics for editors and authors

Login to your personal dashboard for more detailed statistics on your publications.

Access personal reporting

Related Content

This Book

Next chapter

An Overview of Salmonella Biofilms and the Use of Bacteriocins and Bacteriophages as New Control Alternatives

By Alexandre Lamas, Patricia Regal, Laura Sanjulián, Aroa López-Santamarina, Carlos Manuel Franco and Alberto Cepeda

Related Book

First chapter

Introductory Chapter: The Contribution of Cohort Studies to Health Sciences

By René Mauricio Barría

We are IntechOpen, the world's leading publisher of Open Access books. Built by scientists, for scientists. Our readership spans scientists, professors, researchers, librarians, and students, as well as business professionals. We share our knowledge and peer-reveiwed research papers with libraries, scientific and engineering societies, and also work with corporate R&D departments and government entities.

More About Us